Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0005753

Physical Location & Seq

Genomic Coordinates Reverse (-)

5154399 .. 5217900

Gene Structure 63502 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0040500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1119 bp

ATGGCCAATACTGAAGAGCGTCTGCAACAGCTAAAGGCCTTTGACGAATCCAAAGCTGGGGTCAAAGGTCTTGTCGATGATGGAATCACCAAAGTCCCATCCATTTTCATGTACAAGCCAGAAGATCCCTCAGCCGAGGACATCCCAAGTTTCGGCAATCCAACTAGCCAACACCAATTCGGTATTCCAGTTGTGGACCTTGGTGATGCTCTCGCTGGAAGGCCTGCTAAGGTGATCCCAGATATCCGGCGGGCTGCAGAGAGTGTGGGGTTCTTTCATGTGGTTAACCATGGGATACCCAAGAGAGTGTTGGAGGAGATGCTCGAGGCGACGCGTGGGTTTCATGAGCTGCCAAGGGAGGTGAAGGCTGAGTACTATACTAGGGATCCCATGAGGAAGGTCAAATACTTCAGTAGCTATAATTTGTACCAGTCAAGATTTGCTACCTGGAGGGACAGCATGTTTTTTGACATGGCTCCCGAGCCTCTTGATCCACAAGAATTGCCTCTGGTTTGCAGGGATATAACTATGGAGTTTTCTGAGCAAGTACAAAAATTAGCTGTCACTGTGTTTGAATTGCTATCTCAGGCACTTGGACTCAAACCTGACCATCTTTTCGGTTTGGACTGCGAAAAAGAGCATCTTATTATTAGTCACTACTATCCTCCATGTCCTGAGCCTGAGCTCACAATTGGCACAGCCAAACACCAAGATGGCACTTTCATAACACTTCTACTTCAAGATCATATTGGTGGACTTCAGGTTTTGCATGAAAACCAGTGGATTGATGTTCTTCCTCTGCCTGGAGCTCTAATAGTCAACATTGGAAATCTTATGCAGATAATCTCGAATGATAAGTTCGTAAGTGTTGATCATAGAGTGTTGGCAAAGAAAGAAGGTCCAAGAGTATCTGTGGGATGTTTTTTCCGACCTGCGACGTCGCATAATTCGTCGAGGCTGTCAAAAAAGGGCCAAAACAAGTGTCCAAGAAAGCAACGTGGAAGCCTCCATCTGCAGATGCAAATCCCTACTTTGGCGGTAGTCGTATCCAAGGCGGTTCAAGGCTCAAGGACCGAAATTCCCTTTCATGGTATTCCTACTCCGGTTCAAGGGCCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0010033 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0046983 GO:0050789 GO:0050793 GO:0050896 GO:0065007 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GAGA_bind

Protein Analysis

372

Amino Acids

41.66

Weight (kDa)

6.54

Isoelectric Point (pI)

39.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	62 - 165	6.7e-19	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	213 - 311	3.7e-31	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005753)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21240 AT2G21240
fragaria_vesca	FvH4_2g10650 FvH4_2g10650 FvH4_2g10650
malus_domestica	MD05G1054600.v1.1 MD10G1062100.v1.1
prunus_persica	Prupe.8G082900_v2.0.a1 Prupe.8G082900_v2.0.a1 Prupe.8G082900_v2.0.a1 Prupe.8G082900_v2.0.a1 Prupe.8G082900_v2.0.a1 Prupe.8G082900_v2.0.a1 Prupe.8G082900_v2.0.a1 Prupe.8G082900_v2.0.a1
pyrus_communis	pycom05g04480 pycom10g04780
rosa_chinensis	RchiOBHm_Chr6g0268351
rosa_laevigata	RLG00000013918
rosa_multiflora	Rmu_co8407519.1_g000001 Rmu_sc0010202.1_g000012
rosa_roxburghii	Rroxscaffold_7G00199850
rosa_rugosa	Rorug06G0040500
rosa_samantha	Rh6AG160600 Rh6BG165000 Rh6CG153400 Rh6DG150700
rosa_wichuraiana	Rw6G013900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	941
Acc36I	ACCTGC	1 cut(s)	940
AccII	CGCG	1 cut(s)	334
AciI	CCGC	3 cut(s)	250, 1037, 1055
AclWI	GGATC	5 cut(s)	119, 229, 380, 393, 485
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	1 cut(s)	1077
AcuI	CTGAAG	3 cut(s)	33, 394, 743
AcyI	GRCGYC	1 cut(s)	938
AfaI	GTAC	4 cut(s)	113, 374, 428, 549
AfiI	CCNNNNNNNGG	6 cut(s)	57, 152, 803, 1033, 1088, 1109
AflIII	ACRYGT	1 cut(s)	332
AgsI	TTSAA	4 cut(s)	575, 740, 1061, 1109
AjnI	CCWGG	2 cut(s)	446, 802
AjuI	GAANNNNNNNTTGG	2 cut(s)	168, 200
AluBI	AGCT	7 cut(s)	31, 56, 349, 417, 560, 685, 809
AluI	AGCT	7 cut(s)	31, 56, 349, 417, 560, 685, 809
Alw21I	GWGCWC	2 cut(s)	687, 811
AlwI	GGATC	5 cut(s)	119, 229, 380, 393, 485
Ama87I	CYCGRG	2 cut(s)	323, 479
AoxI	GGCC	5 cut(s)	3, 36, 221, 970, 1112
ApeKI	GCWGC	2 cut(s)	254, 349
ApoI	RAATTY	1 cut(s)	1077
ArsI	GACNNNNNNTTYG	8 cut(s)	45, 57, 77, 89, 599, 631, 967, 999
AspS9I	GGNCC	5 cut(s)	196, 899, 970, 1071, 1112
AsuHPI	GGTGA	4 cut(s)	79, 215, 244, 373
AvaI	CYCGRG	2 cut(s)	323, 479
AvaII	GGWCC	3 cut(s)	196, 899, 1071
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	385
BanII	GRGCYC	2 cut(s)	687, 811
BarI	GAAGNNNNNNTAC	4 cut(s)	356, 388, 389, 421
Bbv12I	GWGCWC	2 cut(s)	687, 811
BbvCI	CCTCAGC	1 cut(s)	130
BbvI	GCAGC	2 cut(s)	241, 336
BccI	CCATC	5 cut(s)	74, 106, 618, 707, 1017
BceAI	ACGGC	1 cut(s)	1099
BciT130I	CCWGG	2 cut(s)	448, 804
BciVI	GTATCC	2 cut(s)	288, 1057
BclI	TGATCA	1 cut(s)	871
BfaI	CTAG	2 cut(s)	165, 381
BfmI	CTRYAG	2 cut(s)	255, 1013
BfuAI	ACCTGC	1 cut(s)	940
BfuI	GTATCC	2 cut(s)	288, 1057
BisI	GCNGC	2 cut(s)	255, 350
BlsI	GCNGC	2 cut(s)	256, 351
BmcAI	AGTACT	1 cut(s)	374
Bme1390I	CCNGG	2 cut(s)	448, 804
Bme18I	GGWCC	3 cut(s)	196, 899, 1071
BmeT110I	CYCGRG	2 cut(s)	323, 479
BmgT120I	GGNCC	5 cut(s)	196, 899, 970, 1071, 1112
BmiI	GGNNCC	2 cut(s)	387, 477
BmrFI	CCNGG	2 cut(s)	448, 804
BmsI	GCATC	4 cut(s)	196, 309, 649, 1008
BpmI	CTGGAG	2 cut(s)	469, 825
Bpu10I	CCTNAGC	4 cut(s)	130, 228, 675, 681
BpuEI	CTTGAG	1 cut(s)	1051
BsaBI	GATNNNNATC	1 cut(s)	1022
BsaHI	GRCGYC	1 cut(s)	938
BsaJI	CCNNGG	5 cut(s)	135, 199, 289, 353, 1050
BsaWI	WCCGGW	1 cut(s)	1102
Bsc4I	CCNNNNNNNGG	6 cut(s)	57, 152, 803, 1033, 1088, 1109
Bse1I	ACTGG	3 cut(s)	188, 430, 778
Bse8I	GATNNNNATC	1 cut(s)	1022
BseBI	CCWGG	2 cut(s)	448, 804
BseDI	CCNNGG	5 cut(s)	135, 199, 289, 353, 1050
BseGI	GGATG	3 cut(s)	98, 141, 923
BseJI	GATNNNNATC	1 cut(s)	1022
BseLI	CCNNNNNNNGG	6 cut(s)	57, 152, 803, 1033, 1088, 1109
BseMII	CTCAG	6 cut(s)	144, 360, 531, 599, 666, 672
BseNI	ACTGG	3 cut(s)	188, 430, 778
BseRI	GAGGAG	1 cut(s)	329
BseXI	GCAGC	2 cut(s)	241, 336
BseYI	CCCAGC	1 cut(s)	56
Bsh1236I	CGCG	1 cut(s)	334
BshFI	GGCC	5 cut(s)	5, 38, 223, 972, 1114
BsiHKAI	GWGCWC	2 cut(s)	687, 811
BsiHKCI	CYCGRG	2 cut(s)	323, 479
BsiSI	CCGG	2 cut(s)	247, 1103
BslFI	GGGAC	2 cut(s)	80, 467
BslI	CCNNNNNNNGG	6 cut(s)	57, 152, 803, 1033, 1088, 1109
BsmFI	GGGAC	2 cut(s)	80, 467
BsnI	GGCC	5 cut(s)	5, 38, 223, 972, 1114
BsoBI	CYCGRG	2 cut(s)	323, 479
Bsp1286I	GDGCHC	2 cut(s)	687, 811
Bsp1407I	TGTACA	1 cut(s)	111
Bsp143I	GATC	6 cut(s)	124, 234, 385, 490, 742, 871
Bsp19I	CCATGG	1 cut(s)	289
BspACI	CCGC	3 cut(s)	250, 1037, 1055
BspANI	GGCC	5 cut(s)	5, 38, 223, 972, 1114
BspCNI	CTCAG	6 cut(s)	143, 361, 532, 598, 667, 673
BspFNI	CGCG	1 cut(s)	334
BspHI	TCATGA	1 cut(s)	343
BspLI	GGNNCC	2 cut(s)	387, 477
BspMAI	CTGCAG	2 cut(s)	259, 1017
BspMI	ACCTGC	1 cut(s)	940
BspPI	GGATC	5 cut(s)	119, 229, 380, 393, 485
BspQI	GCTCTTC	1 cut(s)	9
BsrGI	TGTACA	1 cut(s)	111
BsrI	ACTGG	3 cut(s)	188, 430, 778
BssECI	CCNNGG	5 cut(s)	135, 199, 289, 353, 1050
BssMI	GATC	6 cut(s)	124, 234, 385, 490, 742, 871
BssNI	GRCGYC	1 cut(s)	938
BssT1I	CCWWGG	4 cut(s)	199, 289, 353, 1050
Bst2UI	CCWGG	2 cut(s)	448, 804
Bst4CI	ACNGT	1 cut(s)	568
Bst6I	CTCTTC	1 cut(s)	9
BstACI	GRCGYC	1 cut(s)	938
BstAUI	TGTACA	1 cut(s)	111
BstC8I	GCNNGC	2 cut(s)	225, 252
BstDEI	CTNAG	7 cut(s)	130, 228, 369, 540, 585, 675, 681
BstDSI	CCRYGG	1 cut(s)	289
BstF5I	GGATG	3 cut(s)	98, 141, 923
BstFNI	CGCG	1 cut(s)	334
BstKTI	GATC	6 cut(s)	127, 237, 388, 493, 745, 874
BstMBI	GATC	6 cut(s)	124, 234, 385, 490, 742, 871
BstNI	CCWGG	2 cut(s)	448, 804
BstNSI	RCATGY	1 cut(s)	463
BstSCI	CCNGG	2 cut(s)	446, 802
BstSFI	CTRYAG	2 cut(s)	255, 1013
BstUI	CGCG	1 cut(s)	334
BstV1I	GCAGC	2 cut(s)	241, 336
BstX2I	RGATCY	2 cut(s)	124, 385
BstYI	RGATCY	2 cut(s)	124, 385
BsuI	GTATCC	2 cut(s)	288, 1057
BsuRI	GGCC	5 cut(s)	5, 38, 223, 972, 1114
BtgI	CCRYGG	1 cut(s)	289
BtsCI	GGATG	3 cut(s)	98, 141, 923
BtsIMutI	CAGTG	2 cut(s)	564, 785
BveI	ACCTGC	1 cut(s)	940
Cac8I	GCNNGC	2 cut(s)	225, 252
CciI	TCATGA	1 cut(s)	343
Cfr13I	GGNCC	5 cut(s)	196, 899, 970, 1071, 1112
CseI	GACGC	2 cut(s)	8, 340
Csp6I	GTAC	4 cut(s)	112, 373, 427, 548
CspCI	CAANNNNNGTGG	2 cut(s)	483, 518
CviQI	GTAC	4 cut(s)	112, 373, 427, 548
DdeI	CTNAG	7 cut(s)	130, 228, 369, 540, 585, 675, 681
DpnI	GATC	6 cut(s)	126, 236, 387, 492, 744, 873
DpnII	GATC	6 cut(s)	124, 234, 385, 490, 742, 871
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	1 cut(s)	9
EarI	CTCTTC	1 cut(s)	9
Ecl136II	GAGCTC	2 cut(s)	685, 809
Eco130I	CCWWGG	4 cut(s)	199, 289, 353, 1050
Eco147I	AGGCCT	2 cut(s)	38, 223
Eco24I	GRGCYC	2 cut(s)	687, 811
Eco32I	GATATC	1 cut(s)	244
Eco47I	GGWCC	3 cut(s)	196, 899, 1071
Eco53kI	GAGCTC	2 cut(s)	685, 809
Eco57I	CTGAAG	3 cut(s)	33, 394, 743
Eco88I	CYCGRG	2 cut(s)	323, 479
EcoICRI	GAGCTC	2 cut(s)	685, 809
EcoRII	CCWGG	2 cut(s)	446, 802
EcoRV	GATATC	1 cut(s)	244
EcoT14I	CCWWGG	4 cut(s)	199, 289, 353, 1050
EcoT38I	GRGCYC	2 cut(s)	687, 811
ErhI	CCWWGG	4 cut(s)	199, 289, 353, 1050
FalI	AAGNNNNNCTT	2 cut(s)	627, 659
FaqI	GGGAC	2 cut(s)	80, 467
FauI	CCCGC	1 cut(s)	243
FbaI	TGATCA	1 cut(s)	871
Fnu4HI	GCNGC	2 cut(s)	255, 350
FokI	GGATG	3 cut(s)	85, 128, 930
FriOI	GRGCYC	2 cut(s)	687, 811
Fsp4HI	GCNGC	2 cut(s)	255, 350
FspBI	CTAG	2 cut(s)	165, 381
GluI	GCNGC	2 cut(s)	255, 350
GsaI	CCCAGC	1 cut(s)	60
GsuI	CTGGAG	2 cut(s)	469, 825
HaeIII	GGCC	5 cut(s)	5, 38, 223, 972, 1114
HapII	CCGG	2 cut(s)	247, 1103
HgaI	GACGC	2 cut(s)	8, 340
Hin1I	GRCGYC	1 cut(s)	938
HincII	GTYRAC	2 cut(s)	286, 820
HindII	GTYRAC	2 cut(s)	286, 820
HinfI	GANTC	3 cut(s)	47, 84, 597
HpaI	GTTAAC	1 cut(s)	286
HpaII	CCGG	2 cut(s)	247, 1103
HphI	GGTGA	4 cut(s)	79, 215, 244, 373
Hpy166II	GTNNAC	4 cut(s)	196, 286, 755, 820
Hpy188I	TCNGA	2 cut(s)	541, 929
Hpy188III	TCNNGA	7 cut(s)	344, 435, 479, 488, 674, 740, 847
Hpy8I	GTNNAC	4 cut(s)	196, 286, 755, 820
Hpy99I	CGWCG	4 cut(s)	334, 940, 943, 955
HpyAV	CCTTC	4 cut(s)	213, 358, 391, 890
HpyCH4III	ACNGT	1 cut(s)	568
HpyCH4IV	ACGT	2 cut(s)	938, 997
HpyCH4V	TGCA	7 cut(s)	25, 257, 516, 769, 838, 1015, 1021
HpyF3I	CTNAG	7 cut(s)	130, 228, 369, 540, 585, 675, 681
HpySE526I	ACGT	2 cut(s)	938, 997
Hsp92I	GRCGYC	1 cut(s)	938
Ksp22I	TGATCA	1 cut(s)	871
KspAI	GTTAAC	1 cut(s)	286
Kzo9I	GATC	6 cut(s)	124, 234, 385, 490, 742, 871
LguI	GCTCTTC	1 cut(s)	9
LmnI	GCTCC	2 cut(s)	481, 806
Lsp1109I	GCAGC	2 cut(s)	241, 336
LweI	GCATC	4 cut(s)	196, 309, 649, 1008
MaeI	CTAG	2 cut(s)	165, 381
MaeII	ACGT	2 cut(s)	938, 997
MaeIII	GTNAC	2 cut(s)	562, 653
MalI	GATC	6 cut(s)	126, 236, 387, 492, 744, 873
MboI	GATC	6 cut(s)	124, 234, 385, 490, 742, 871
MboII	GAAGA	3 cut(s)	26, 134, 785
MfeI	CAATTG	1 cut(s)	690
MflI	RGATCY	2 cut(s)	124, 385
MhlI	GDGCHC	2 cut(s)	687, 811
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	8 cut(s)	176, 421, 500, 554, 575, 690, 946, 1077
MluI	ACGCGT	1 cut(s)	332
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	591
MmeI	TCCRAC	3 cut(s)	185, 291, 952
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	1 cut(s)	285
MslI	CAYNNNNRTG	3 cut(s)	107, 711, 750
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	2 cut(s)	247, 1103
MspR9I	CCNGG	2 cut(s)	448, 804
MunI	CAATTG	1 cut(s)	690
MvaI	CCWGG	2 cut(s)	448, 804
MvnI	CGCG	1 cut(s)	334
NcoI	CCATGG	1 cut(s)	289
NdeII	GATC	6 cut(s)	124, 234, 385, 490, 742, 871
NlaIV	GGNNCC	2 cut(s)	387, 477
NmeAIII	GCCGAG	1 cut(s)	160
NmuCI	GTSAC	2 cut(s)	562, 653
NspI	RCATGY	1 cut(s)	463
PaeR7I	CTCGAG	1 cut(s)	323
PagI	TCATGA	1 cut(s)	343
PceI	AGGCCT	2 cut(s)	38, 223
PciSI	GCTCTTC	1 cut(s)	9
PcsI	WCGNNNNNNNCGW	1 cut(s)	947
PfeI	GAWTC	2 cut(s)	47, 84
PkrI	GCNGC	2 cut(s)	256, 351
PleI	GAGTC	1 cut(s)	591
PpsI	GAGTC	1 cut(s)	591
Psp124BI	GAGCTC	2 cut(s)	687, 811
Psp6I	CCWGG	2 cut(s)	446, 802
PspFI	CCCAGC	1 cut(s)	56
PspGI	CCWGG	2 cut(s)	446, 802
PspN4I	GGNNCC	2 cut(s)	387, 477
PspPI	GGNCC	5 cut(s)	196, 899, 970, 1071, 1112
PspXI	VCTCGAGB	1 cut(s)	323
PstI	CTGCAG	2 cut(s)	259, 1017
PsuI	RGATCY	2 cut(s)	124, 385
RsaI	GTAC	4 cut(s)	113, 374, 428, 549
RsaNI	GTAC	4 cut(s)	112, 373, 427, 548
RseI	CAYNNNNRTG	3 cut(s)	107, 711, 750
SacI	GAGCTC	2 cut(s)	687, 811
SapI	GCTCTTC	1 cut(s)	9
SaqAI	TTAA	1 cut(s)	285
SatI	GCNGC	2 cut(s)	255, 350
Sau3AI	GATC	6 cut(s)	124, 234, 385, 490, 742, 871
Sau96I	GGNCC	5 cut(s)	196, 899, 970, 1071, 1112
ScaI	AGTACT	1 cut(s)	374
SchI	GAGTC	1 cut(s)	591
ScrFI	CCNGG	2 cut(s)	448, 804
SduI	GDGCHC	2 cut(s)	687, 811
SfaNI	GCATC	4 cut(s)	196, 309, 649, 1008
SfcI	CTRYAG	2 cut(s)	255, 1013
Sfr274I	CTCGAG	1 cut(s)	323
SinI	GGWCC	3 cut(s)	196, 899, 1071
SlaI	CTCGAG	1 cut(s)	323
SmiMI	CAYNNNNRTG	3 cut(s)	107, 711, 750
SmlI	CTYRAG	2 cut(s)	323, 1066
SmoI	CTYRAG	2 cut(s)	323, 1066
Sse9I	AATT	8 cut(s)	176, 421, 500, 554, 575, 690, 946, 1077
SseBI	AGGCCT	2 cut(s)	38, 223
SsiI	CCGC	3 cut(s)	250, 1037, 1055
SspMI	CTAG	2 cut(s)	165, 381
SstI	GAGCTC	2 cut(s)	687, 811
StuI	AGGCCT	2 cut(s)	38, 223
StyD4I	CCNGG	2 cut(s)	446, 802
StyI	CCWWGG	4 cut(s)	199, 289, 353, 1050
TaaI	ACNGT	1 cut(s)	568
TaiI	ACGT	2 cut(s)	941, 1000
TaqI	TCGA	4 cut(s)	75, 324, 848, 953
TaqII	GACCGA	1 cut(s)	1088
TasI	AATT	8 cut(s)	176, 421, 500, 554, 575, 690, 946, 1077
TatI	WGTACW	3 cut(s)	111, 372, 547
TfiI	GAWTC	2 cut(s)	47, 84
Tru1I	TTAA	1 cut(s)	285
Tru9I	TTAA	1 cut(s)	285
TscAI	CASTG	2 cut(s)	571, 785
TseFI	GTSAC	2 cut(s)	562, 653
TseI	GCWGC	2 cut(s)	254, 349
Tsp45I	GTSAC	2 cut(s)	562, 653
TspDTI	ATGAA	6 cut(s)	97, 266, 332, 712, 786, 1076
TspRI	CASTG	2 cut(s)	571, 785
VpaK11BI	GGWCC	3 cut(s)	196, 899, 1071
XapI	RAATTY	1 cut(s)	1077
XceI	RCATGY	1 cut(s)	463
XcmI	CCANNNNNNNNNTGG	1 cut(s)	307
XhoI	CTCGAG	1 cut(s)	323
XspI	CTAG	2 cut(s)	165, 381
ZraI	GACGTC	1 cut(s)	939
ZrmI	AGTACT	1 cut(s)	374

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.