pycom05g05860

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011365
Physical Location & Seq
Forward (+)
7360307 .. 7366703
6397 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2748 bp
ATGGCTCAGCCTCTCGTGAAGAAGGACGACGATCGCGACGATGAAGAGTACTCGCCATTTCTGGGGATTGAGAAGGGTGCCGTTCTTCAGGAAGCGAGAGTTTTCAATGATCCTCAACTCGATGCTAGAAGATGCTCCCAGGTTATCACAAAACTTTTGTACCTACTGAACCAGGGGGAAAGCTTTACAAAGGTTGAAGCTACAGAAGTTTTCTTTTCTGTGACTAAGCTTTTCCAATCAAGAGATATAGGATTGAGGAGAATGGTTTACCTGATCATAAAAGAATTGTCCCCCTCTGCAGATGAGGTTATTATTGTGACAAGCTCCCTGATGAAAGATATGAATAGTAAAACCGACATGTATCGGGCAAATGCCATCCGTGTGCTCTGTCAGATTACGGATGGAACCCTTCTTACCCAGATTGAGCGGTATTTGAAACAAGCCATTGTTGATAAGAATCCAGTGGTTGCGAGTGCAGCTCTAGTCAGTGGGATCCATCTGCTCCAGACTAACCCAGAGATTGTCAAAAGGTGGAGTAATGAGGTTCAGGACGCTGTCCAGTCAAGAGCAGCCCTTGTACAATTCCATGCAGTTGCTTTACTTCATCAGATACGACAGAATGACCGTCTTGCTGTTAGCAAGTTGGTTACCAGCTTGACAAGGGGGACAGTCCGCTCTCCATTGGCCCAGTGCTTGTTGATTCGTTATACTAGTCAGGTTATCCGCGAGTCTGGAAATGTACAAACAGGGGACCGTCCCTTTTACGATTATCTTGAGGGTTGTCTTCGCCACAAATCAGAGATGGTGATTTTTGAGGCTGCTAGAGCAATTACAGAGCTCCATGGTGTGACCCCCAGAGAACTTACTCCAGCAATCACTGTTCTGCAGCTATTTCTAAGTTCTTCCAAACCAGTTTTAAGATTTGCTGCTGTCCGCACCTTGAATAAGGTGGCAATGACACATCCAGTGCCTGTCACAAACTGCAACATTGATATGGAGAGTTTGATTTCTGACCAGAACAGAAGCATTGCCACACTTGCAATCACAACACTTCTGAAGACAGGAAACGAATCAAGTGTGGATCGCTTGATGAAGCAGATAACTAATTTCATGTCAGATATTGCTGACGAGTTCAAAATTGTTGTTGTGGAAGCCATAAGATCATTGTGTTTGAAATTTCCATTGAAGTTCAGAGCTTTGATGAACTTTTTGAGCAACATTCTCAGAGAAGAAGGTGGATTTGAGTACAAAAAGGCAATTGTTGATTCAATTGTGATTCTCATAAGAGATATTCCTGATGCAAAAGAAAGTGGCTTGCTTCATCTCTGTGAGTTCATCGAAGATTGTGAATTCACCTATCTTTCTACACAGATACTCCACTTTTTGGGTATTGAAGGGCCAAAAACATCTGATCCAAGCAAATATATACGGTACATTTATAATCGAGTACATCTTGAGAATGCCACTGTTCGTGCCAGTGCGGTTAGTACATTGGCAAAATTTGGTGCTTTGGTTGATTCATTGAAGGTAACATCTTGTTCAATGAATGATATCTTATCTGATCCCCCTCCCCCTCTATACAAAGAAGATTTGTTTCTGTCTATGGTTACTGATAACAATGAACTATACTTGTTTTCTTCTATGCTTCAGCCACGAGTGTTTATTCTGCTGCGAAGATGTCTCTTTGACAGTGATGATGAGGTTCGTGATAGGGCAACACTCTATCTAAATACGCTTGGCGGAGATGGTTCAGTTGTTGAGACTGACAATGATGTAAAGGACTTCCTCTTTGGGTCTCTGGATGTCCCACTTGTCAATCTGGAGGCAAGTTTGAAGAATTATGAGGCTTCGGAAGAACCATTTGACATTAAATCTGTACCCAAGGAGGTTAAGTCGCAGCCACTTGCTGAGAAGAAAGCCCAGAGTAAAAAGCCAACTGGTCTTGGTGCTCCTCCAACTGGCCCTGCATCCACAGTTGATGCTTATGAGACGCTGCTTTCCTCTATTCCGGAGTTTTCAAACTTTGGGAAACTATTCAAGGTTCTGCAACTTAATGTTGTCAAGCACATTTTTGATAGCCATGTTGTGTTCCAGTACAACTGCACCAATACCATTCCCGAGCAATTACTGGAAGATGTCATTGTCGTGGTGGATGCTTCAGAAGCTGAGGAATTTTCTGAAGTGGCATCCAGGTCTCTCAGGTCTCTTCCGTATGATACACCTGGACAAACCTTTTTGGCATTTGAGAAGCCAGAAGGTGTCCCTGCAGTTGGAAAATTTACAAACTTGTTGAGGTTCATTGTTAAAGAGGTTGATCCAACCACTGGTGAGGCAGAGGAAGATGGTGTGGAAGATGAATACCAGCTGGACGATCTTGAGGTTGTCGCTGCTGATTACATTTTGAAGGTTCCAGTCTTCAATTTCAAGAATGCTTGGGAAAGCATGGGCGCAGATTTTGAGCGGGTAGATGAATATGGCCTGGGGCCAAGGGAGAACTTGACTGAAGCTGTTAATGCTGTCATCAGCCTCCTTGGAATGCAACCCTGTGAGGGCACAGAGGTCGTTGCGAGCAACTCAAGGTCACACACTTGTGTATTGTCCGGTGTATATATAGGCAATGTGAAGGTCCTTGTGCGGTTGTTATTTGGAATTGATAGTTCATCAAAGGAGGTTGCGATGAAACTCGCTGTGAGATCTGAGGACGGAGCTGTTAGTGATGCGATTCATGAGATTGTAGCCAGCGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adaptin_N COP-gamma_platf Coatomer_g_Cpla

Protein Analysis

916

Amino Acids

101.79

Weight (kDa)

5.04

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 26 - 579 1.6e-136 Adaptin N terminal region
Cnd1 PF12717 122 - 217 1.6e-06 non-SMC mitotic condensation complex subunit 1
COP-gamma_platf PF08752 661 - 794 1.8e-35 Coatomer gamma subunit appendage platform subdomain
Coatomer_g_Cpla PF16381 797 - 912 6e-35 Coatomer subunit gamma-1 C-terminal appendage platform
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34450
fragaria_vesca FvH4_2g08660 FvH4_2g08660 FvH4_2g08660
malus_domestica MD05G1067500.v1.1 MD10G1077400.v1.1
prunus_persica Prupe.8G098200_v2.0.a1
pyrus_communis pycom05g05860 pycom10g06040
rosa_chinensis RchiOBHm_Chr6g0264051
rosa_laevigata RLG00000014293
rosa_multiflora Rmu_sc0006339.1_g000005
rosa_roxburghii Rroxscaffold_176G00431390 Rroxscaffold_7G00203630
rosa_rugosa Rorug06G0007600
rosa_samantha Rh6AG128800 Rh6BG124900 Rh6CG123600 Rh6DG110600
rosa_wichuraiana Rw6G011130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1440
AccB1I GGYRCC 1 cut(s) 77
AccB7I CCANNNNNTGG 2 cut(s) 1384, 2324
AccBSI CCGCTC 3 cut(s) 427, 675, 2461
AccII CGCG 2 cut(s) 36, 726
AccIII TCCGGA 1 cut(s) 2008
AciI CCGC 9 cut(s) 427, 673, 724, 934, 1481, 1740, 2461, 2635, 2742
AclWI GGATC 7 cut(s) 104, 487, 500, 1089, 1406, 1556, 2309
AcsI RAATTY 5 cut(s) 1175, 1349, 1499, 2171, 2276
AcuI CTGAAG 6 cut(s) 71, 1076, 1631, 2142, 2199, 2523
AfiI CCNNNNNNNGG 6 cut(s) 62, 1384, 1958, 2270, 2324, 2549
AflIII ACRYGT 1 cut(s) 357
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 5 cut(s) 138, 171, 2189, 2221, 2478
AleI CACNNNNGTG 1 cut(s) 2589
Alw21I GWGCWC 3 cut(s) 387, 840, 1951
Alw26I GTCTC 6 cut(s) 1685, 1754, 1800, 1982, 2199, 2208
AlwI GGATC 7 cut(s) 104, 487, 500, 1089, 1406, 1556, 2309
AlwNI CAGNNNCTG 2 cut(s) 971, 2165
Ama87I CYCGRG 1 cut(s) 2117
Aor13HI TCCGGA 1 cut(s) 2008
AoxI GGCC 5 cut(s) 684, 1397, 1960, 2476, 2483
ApeKI GCWGC 9 cut(s) 476, 569, 818, 886, 926, 1669, 1897, 1993, 2387
ApoI RAATTY 5 cut(s) 1175, 1349, 1499, 2171, 2276
ArsI GACNNNNNNTTYG 2 cut(s) 1772, 1804
Asp700I GAANNNNTTC 1 cut(s) 2033
AspLEI GCGC 1 cut(s) 2450
AspS9I GGNCC 6 cut(s) 685, 751, 1397, 1961, 2483, 2626
AsuHPI GGTGA 3 cut(s) 817, 1345, 2339
AvaI CYCGRG 1 cut(s) 2117
AvaII GGWCC 2 cut(s) 751, 2626
BaeGI GKGCMC 1 cut(s) 2555
BaeI ACNNNNGTAYC 2 cut(s) 143, 176
BamHI GGATCC 1 cut(s) 492
BanI GGYRCC 1 cut(s) 77
BanII GRGCYC 1 cut(s) 840
BauI CACGAG 2 cut(s) 14, 1653
BbsI GAAGAC 3 cut(s) 776, 1064, 2407
Bbv12I GWGCWC 3 cut(s) 387, 840, 1951
BbvCI CCTCAGC 1 cut(s) 2166
BbvI GCAGC 9 cut(s) 488, 581, 805, 898, 913, 1656, 1909, 1980, 2374
BccI CCATC 6 cut(s) 383, 395, 504, 796, 1739, 2336
BceAI ACGGC 1 cut(s) 65
BcgI CGANNNNNNTGC 2 cut(s) 2542, 2576
BciT130I CCWGG 5 cut(s) 140, 173, 2191, 2223, 2480
BclI TGATCA 1 cut(s) 273
BcoDI GTCTC 6 cut(s) 1685, 1754, 1800, 1982, 2199, 2208
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 4 cut(s) 126, 482, 711, 822
BfmI CTRYAG 4 cut(s) 201, 297, 884, 2265
BglII AGATCT 1 cut(s) 2693
BisI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1670, 1898, 1994, 2388
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 9 cut(s) 478, 571, 820, 888, 928, 1671, 1899, 1995, 2389
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 5 cut(s) 140, 173, 2191, 2223, 2480
Bme18I GGWCC 2 cut(s) 751, 2626
BmeT110I CYCGRG 1 cut(s) 2117
BmgT120I GGNCC 6 cut(s) 685, 751, 1397, 1961, 2483, 2626
BmiI GGNNCC 6 cut(s) 79, 406, 494, 752, 2409, 2484
BmrFI CCNGG 5 cut(s) 140, 173, 2191, 2223, 2480
BmrI ACTGGG 1 cut(s) 682
BmsI GCATC 8 cut(s) 112, 122, 1288, 1969, 1976, 2143, 2195, 2707
BmuI ACTGGG 1 cut(s) 682
BpiI GAAGAC 3 cut(s) 776, 1064, 2407
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpmI CTGGAG 3 cut(s) 488, 852, 1841
Bpu10I CCTNAGC 1 cut(s) 2166
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 4 cut(s) 794, 1475, 2396, 2560
BsaBI GATNNNNATC 1 cut(s) 456
BsaI GGTCTC 3 cut(s) 1800, 2199, 2208
BsaJI CCNNGG 7 cut(s) 138, 172, 841, 1881, 2479, 2486, 2530
BsaWI WCCGGW 2 cut(s) 2008, 2600
BsaXI ACNNNNNCTCC 4 cut(s) 1359, 1389, 1877, 1907
Bsc4I CCNNNNNNNGG 6 cut(s) 62, 1384, 1958, 2270, 2324, 2549
Bse3DI GCAATG 3 cut(s) 960, 1026, 2623
Bse8I GATNNNNATC 1 cut(s) 456
BseAI TCCGGA 1 cut(s) 2008
BseBI CCWGG 5 cut(s) 140, 173, 2191, 2223, 2480
BseDI CCNNGG 7 cut(s) 138, 172, 841, 1881, 2479, 2486, 2530
BseGI GGATG 7 cut(s) 375, 406, 961, 1807, 1967, 2158, 2186
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 6 cut(s) 62, 1384, 1958, 2270, 2324, 2549
BseMI GCAATG 3 cut(s) 960, 1026, 2623
BseMII CTCAG 6 cut(s) 20, 1237, 1899, 2157, 2212, 2688
BseRI GAGGAG 2 cut(s) 271, 1941
BseSI GKGCMC 1 cut(s) 2555
BseXI GCAGC 9 cut(s) 488, 581, 805, 898, 913, 1656, 1909, 1980, 2374
BsgI GTGCAG 2 cut(s) 495, 2086
Bsh1236I CGCG 2 cut(s) 36, 726
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 5 cut(s) 686, 1399, 1962, 2478, 2485
BshNI GGYRCC 1 cut(s) 77
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 3 cut(s) 387, 840, 1951
BsiHKCI CYCGRG 1 cut(s) 2117
BsiSI CCGG 2 cut(s) 2009, 2601
BslFI GGGAC 6 cut(s) 274, 679, 741, 764, 1790, 2246
BslI CCNNNNNNNGG 6 cut(s) 62, 1384, 1958, 2270, 2324, 2549
BsmAI GTCTC 6 cut(s) 1685, 1754, 1800, 1982, 2199, 2208
BsmBI CGTCTC 1 cut(s) 1982
BsmFI GGGAC 6 cut(s) 274, 679, 741, 764, 1790, 2246
BsmI GAATGC 3 cut(s) 1465, 2434, 2541
BsnI GGCC 5 cut(s) 686, 1399, 1962, 2478, 2485
Bso31I GGTCTC 3 cut(s) 1800, 2199, 2208
BsoBI CYCGRG 1 cut(s) 2117
Bsp1286I GDGCHC 4 cut(s) 387, 840, 1951, 2555
Bsp13I TCCGGA 1 cut(s) 2008
Bsp1407I TGTACA 2 cut(s) 577, 739
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 841
Bsp68I TCGCGA 1 cut(s) 36
BspACI CCGC 9 cut(s) 427, 673, 724, 934, 1481, 1740, 2461, 2635, 2742
BspANI GGCC 5 cut(s) 686, 1399, 1962, 2478, 2485
BspCNI CTCAG 6 cut(s) 19, 1236, 1900, 2158, 2211, 2689
BspEI TCCGGA 1 cut(s) 2008
BspFNI CGCG 2 cut(s) 36, 726
BspHI TCATGA 1 cut(s) 2725
BspLI GGNNCC 6 cut(s) 79, 406, 494, 752, 2409, 2484
BspMAI CTGCAG 3 cut(s) 301, 888, 2269
BspPI GGATC 7 cut(s) 104, 487, 500, 1089, 1406, 1556, 2309
BspT107I GGYRCC 1 cut(s) 77
BspTNI GGTCTC 3 cut(s) 1800, 2199, 2208
BsrBI CCGCTC 3 cut(s) 427, 675, 2461
BsrDI GCAATG 3 cut(s) 960, 1026, 2623
BsrGI TGTACA 2 cut(s) 577, 739
BssECI CCNNGG 7 cut(s) 138, 172, 841, 1881, 2479, 2486, 2530
BssSI CACGAG 2 cut(s) 14, 1653
BssT1I CCWWGG 4 cut(s) 841, 1881, 2486, 2530
Bst2BI CACGAG 2 cut(s) 14, 1653
Bst2UI CCWGG 5 cut(s) 140, 173, 2191, 2223, 2480
Bst4CI ACNGT 8 cut(s) 626, 670, 755, 880, 1431, 1468, 1691, 1975
Bst6I CTCTTC 2 cut(s) 39, 2211
BstAUI TGTACA 2 cut(s) 577, 739
BstC8I GCNNGC 3 cut(s) 1316, 2569, 2740
BstDEI CTNAG 8 cut(s) 6, 225, 896, 1223, 1908, 2166, 2198, 2697
BstDSI CCRYGG 1 cut(s) 841
BstEII GGTNACC 1 cut(s) 646
BstF5I GGATG 7 cut(s) 375, 406, 961, 1807, 1967, 2158, 2186
BstFNI CGCG 2 cut(s) 36, 726
BstHHI GCGC 1 cut(s) 2450
BstMAI GTCTC 6 cut(s) 1685, 1754, 1800, 1982, 2199, 2208
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 5 cut(s) 476, 824, 1037, 2162, 2513
BstNI CCWGG 5 cut(s) 140, 173, 2191, 2223, 2480
BstNSI RCATGY 1 cut(s) 361
BstPI GGTNACC 1 cut(s) 646
BstSCI CCNGG 5 cut(s) 138, 171, 2189, 2221, 2478
BstSFI CTRYAG 4 cut(s) 201, 297, 884, 2265
BstSLI GKGCMC 1 cut(s) 2555
BstUI CGCG 2 cut(s) 36, 726
BstV1I GCAGC 9 cut(s) 488, 581, 805, 898, 913, 1656, 1909, 1980, 2374
BstV2I GAAGAC 3 cut(s) 776, 1064, 2407
BstX2I RGATCY 2 cut(s) 492, 2693
BstYI RGATCY 2 cut(s) 492, 2693
BsuRI GGCC 5 cut(s) 686, 1399, 1962, 2478, 2485
BtgI CCRYGG 1 cut(s) 841
BtgZI GCGATG 1 cut(s) 2690
BtsCI GGATG 7 cut(s) 375, 406, 961, 1807, 1967, 2158, 2186
BtsIMutI CAGTG 9 cut(s) 468, 493, 695, 876, 972, 1464, 1483, 1696, 2322
BtuMI TCGCGA 1 cut(s) 36
Cac8I GCNNGC 3 cut(s) 1316, 2569, 2740
CaiI CAGNNNCTG 2 cut(s) 971, 2165
CciI TCATGA 1 cut(s) 2725
CfoI GCGC 1 cut(s) 2450
Cfr13I GGNCC 6 cut(s) 685, 751, 1397, 1961, 2483, 2626
CseI GACGC 2 cut(s) 560, 1999
CviAII CATG 7 cut(s) 358, 587, 842, 1111, 2081, 2443, 2726
DdeI CTNAG 8 cut(s) 6, 225, 896, 1223, 1908, 2166, 2198, 2697
Eam1104I CTCTTC 2 cut(s) 39, 2211
EarI CTCTTC 2 cut(s) 39, 2211
EciI GGCGGA 1 cut(s) 1755
Ecl136II GAGCTC 1 cut(s) 838
Eco130I CCWWGG 4 cut(s) 841, 1881, 2486, 2530
Eco24I GRGCYC 1 cut(s) 840
Eco31I GGTCTC 3 cut(s) 1800, 2199, 2208
Eco32I GATATC 1 cut(s) 1552
Eco47I GGWCC 2 cut(s) 751, 2626
Eco53kI GAGCTC 1 cut(s) 838
Eco57I CTGAAG 6 cut(s) 71, 1076, 1631, 2142, 2199, 2523
Eco88I CYCGRG 1 cut(s) 2117
Eco91I GGTNACC 1 cut(s) 646
EcoICRI GAGCTC 1 cut(s) 838
EcoO109I RGGNCCY 1 cut(s) 2626
EcoO65I GGTNACC 1 cut(s) 646
EcoRI GAATTC 1 cut(s) 1349
EcoRII CCWGG 5 cut(s) 138, 171, 2189, 2221, 2478
EcoRV GATATC 1 cut(s) 1552
EcoT14I CCWWGG 4 cut(s) 841, 1881, 2486, 2530
EcoT38I GRGCYC 1 cut(s) 840
ErhI CCWWGG 4 cut(s) 841, 1881, 2486, 2530
Esp3I CGTCTC 1 cut(s) 1982
FaeI CATG 7 cut(s) 361, 590, 845, 1114, 2084, 2446, 2729
FaqI GGGAC 6 cut(s) 274, 679, 741, 764, 1790, 2246
FatI CATG 7 cut(s) 357, 586, 841, 1110, 2080, 2442, 2725
FauI CCCGC 2 cut(s) 2454, 2735
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1670, 1898, 1994, 2388
FokI GGATG 7 cut(s) 362, 413, 948, 1814, 1954, 2165, 2173
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1670, 1898, 1994, 2388
FspBI CTAG 4 cut(s) 126, 482, 711, 822
GlaI GCGC 1 cut(s) 2449
GluI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1670, 1898, 1994, 2388
GsuI CTGGAG 3 cut(s) 488, 852, 1841
HaeIII GGCC 5 cut(s) 686, 1399, 1962, 2478, 2485
HapII CCGG 2 cut(s) 2009, 2601
HgaI GACGC 2 cut(s) 560, 1999
HhaI GCGC 1 cut(s) 2450
Hin1II CATG 7 cut(s) 361, 590, 845, 1114, 2084, 2446, 2729
Hin6I GCGC 1 cut(s) 2448
HinP1I GCGC 1 cut(s) 2448
HindIII AAGCTT 2 cut(s) 181, 227
HinfI GANTC 8 cut(s) 457, 700, 728, 1070, 1265, 1276, 1517, 2722
HpaII CCGG 2 cut(s) 2009, 2601
HphI GGTGA 3 cut(s) 817, 1345, 2339
Hpy166II GTNNAC 1 cut(s) 268
Hpy8I GTNNAC 1 cut(s) 268
Hpy99I CGWCG 2 cut(s) 32, 41
HpyAV CCTTC 9 cut(s) 16, 67, 419, 1226, 1388, 1519, 2249, 2398, 2617
HpyCH4III ACNGT 8 cut(s) 626, 670, 755, 880, 1431, 1468, 1691, 1975
HpyF10VI GCNNNNNNNGC 5 cut(s) 476, 824, 1037, 2162, 2513
HpyF3I CTNAG 8 cut(s) 6, 225, 896, 1223, 1908, 2166, 2198, 2697
Hsp92II CATG 7 cut(s) 361, 590, 845, 1114, 2084, 2446, 2729
HspAI GCGC 1 cut(s) 2448
Kpn2I TCCGGA 1 cut(s) 2008
Ksp22I TGATCA 1 cut(s) 273
LmnI GCTCC 6 cut(s) 140, 329, 507, 843, 1954, 2705
Lsp1109I GCAGC 9 cut(s) 488, 581, 805, 898, 913, 1656, 1909, 1980, 2374
LweI GCATC 8 cut(s) 112, 122, 1288, 1969, 1976, 2143, 2195, 2707
MaeI CTAG 4 cut(s) 126, 482, 711, 822
MaeIII GTNAC 8 cut(s) 220, 316, 646, 847, 973, 1528, 1606, 2580
MbiI CCGCTC 3 cut(s) 427, 675, 2461
MfeI CAATTG 2 cut(s) 1257, 1269
MflI RGATCY 2 cut(s) 492, 2693
MhlI GDGCHC 4 cut(s) 387, 840, 1951, 2555
MlyI GAGTC 1 cut(s) 737
MmeI TCCRAC 3 cut(s) 1979, 2251, 2342
MroI TCCGGA 1 cut(s) 2008
MroXI GAANNNNTTC 1 cut(s) 2033
MseI TTAA 6 cut(s) 917, 1869, 1890, 2052, 2304, 2511
MslI CAYNNNNRTG 5 cut(s) 380, 992, 1326, 2144, 2589
MspA1I CMGCKG 2 cut(s) 2365, 2742
MspI CCGG 2 cut(s) 2009, 2601
MspR9I CCNGG 5 cut(s) 140, 173, 2191, 2223, 2480
MunI CAATTG 2 cut(s) 1257, 1269
Mva1269I GAATGC 3 cut(s) 1465, 2434, 2541
MvaI CCWGG 5 cut(s) 140, 173, 2191, 2223, 2480
MvnI CGCG 2 cut(s) 36, 726
MwoI GCNNNNNNNGC 5 cut(s) 476, 824, 1037, 2162, 2513
NcoI CCATGG 1 cut(s) 841
NlaIII CATG 7 cut(s) 361, 590, 845, 1114, 2084, 2446, 2729
NlaIV GGNNCC 6 cut(s) 79, 406, 494, 752, 2409, 2484
NmuCI GTSAC 5 cut(s) 220, 316, 847, 973, 2580
NruI TCGCGA 1 cut(s) 36
NspI RCATGY 1 cut(s) 361
OliI CACNNNNGTG 1 cut(s) 2589
PagI TCATGA 1 cut(s) 2725
PciI ACATGT 1 cut(s) 357
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 36
PctI GAATGC 3 cut(s) 1465, 2434, 2541
PdmI GAANNNNTTC 1 cut(s) 2033
PfeI GAWTC 7 cut(s) 457, 700, 1070, 1265, 1276, 1517, 2722
PflFI GACNNNGTC 1 cut(s) 554
PflMI CCANNNNNTGG 2 cut(s) 1384, 2324
PkrI GCNGC 9 cut(s) 478, 571, 820, 888, 928, 1671, 1899, 1995, 2389
Ple19I CGATCG 1 cut(s) 34
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
PpuMI RGGWCCY 1 cut(s) 2626
PscI ACATGT 1 cut(s) 357
PsiI TTATAA 1 cut(s) 1440
Psp124BI GAGCTC 1 cut(s) 840
Psp5II RGGWCCY 1 cut(s) 2626
Psp6I CCWGG 5 cut(s) 138, 171, 2189, 2221, 2478
PspEI GGTNACC 1 cut(s) 646
PspGI CCWGG 5 cut(s) 138, 171, 2189, 2221, 2478
PspN4I GGNNCC 6 cut(s) 79, 406, 494, 752, 2409, 2484
PspPI GGNCC 6 cut(s) 685, 751, 1397, 1961, 2483, 2626
PspPPI RGGWCCY 1 cut(s) 2626
PsrI GAACNNNNNNTAC 2 cut(s) 397, 429
PstI CTGCAG 3 cut(s) 301, 888, 2269
PstNI CAGNNNCTG 2 cut(s) 971, 2165
PsuI RGATCY 2 cut(s) 492, 2693
PsyI GACNNNGTC 1 cut(s) 554
PvuI CGATCG 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 2365
RruI TCGCGA 1 cut(s) 36
RseI CAYNNNNRTG 5 cut(s) 380, 992, 1326, 2144, 2589
SacI GAGCTC 1 cut(s) 840
SaqAI TTAA 6 cut(s) 917, 1869, 1890, 2052, 2304, 2511
SatI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1670, 1898, 1994, 2388
Sau96I GGNCC 6 cut(s) 685, 751, 1397, 1961, 2483, 2626
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 5 cut(s) 140, 173, 2191, 2223, 2480
SduI GDGCHC 4 cut(s) 387, 840, 1951, 2555
SfaNI GCATC 8 cut(s) 112, 122, 1288, 1969, 1976, 2143, 2195, 2707
SfcI CTRYAG 4 cut(s) 201, 297, 884, 2265
SinI GGWCC 2 cut(s) 751, 2626
SmiMI CAYNNNNRTG 5 cut(s) 380, 992, 1326, 2144, 2589
SmlI CTYRAG 4 cut(s) 773, 1454, 2375, 2575
SmoI CTYRAG 4 cut(s) 773, 1454, 2375, 2575
SpeI ACTAGT 1 cut(s) 710
SsiI CCGC 9 cut(s) 427, 673, 724, 934, 1481, 1740, 2461, 2635, 2742
SspMI CTAG 4 cut(s) 126, 482, 711, 822
SstI GAGCTC 1 cut(s) 840
StyD4I CCNGG 5 cut(s) 138, 171, 2189, 2221, 2478
StyI CCWWGG 4 cut(s) 841, 1881, 2486, 2530
TaaI ACNGT 8 cut(s) 626, 670, 755, 880, 1431, 1468, 1691, 1975
TaqI TCGA 3 cut(s) 120, 1338, 1444
TatI WGTACW 7 cut(s) 48, 577, 739, 1245, 1447, 1487, 2094
TfiI GAWTC 7 cut(s) 457, 700, 1070, 1265, 1276, 1517, 2722
Tru1I TTAA 6 cut(s) 917, 1869, 1890, 2052, 2304, 2511
Tru9I TTAA 6 cut(s) 917, 1869, 1890, 2052, 2304, 2511
TscAI CASTG 9 cut(s) 468, 493, 695, 883, 972, 1471, 1483, 1696, 2329
TseFI GTSAC 5 cut(s) 220, 316, 847, 973, 2580
TseI GCWGC 9 cut(s) 476, 569, 818, 886, 926, 1669, 1897, 1993, 2387
Tsp45I GTSAC 5 cut(s) 220, 316, 847, 973, 2580
TspGWI ACGGA 4 cut(s) 368, 413, 2199, 2718
TspRI CASTG 9 cut(s) 468, 493, 695, 883, 972, 1471, 1483, 1696, 2329
Tth111I GACNNNGTC 1 cut(s) 554
Van91I CCANNNNNTGG 2 cut(s) 1384, 2324
VpaK11BI GGWCC 2 cut(s) 751, 2626
XapI RAATTY 5 cut(s) 1175, 1349, 1499, 2171, 2276
XceI RCATGY 1 cut(s) 361
XmnI GAANNNNTTC 1 cut(s) 2033
XspI CTAG 4 cut(s) 126, 482, 711, 822
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.