pycom05g10080

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004218
Physical Location & Seq
Reverse (-)
13436067 .. 13439999
3933 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1527 bp
ATGTGGGGTGTTAGAGTCAAATTCCAAGCTTTAGTTCATTACTGGCAGAGATTTGCAACAAGAGAAACAGAAGAAGCAAAAATGACGTTCAGTTCCAGCATCTGCACATCTGCTAAATTCTGGGCGTGGCAAGCATCTTTTCTGCTGCTGCTTACCAACAATGGTGCATCACAGTCGATCATCAAGAACTTGCCGGGCTTTTCCGGTGACCTCCCCTTCAAGCTTGAAACAGGGTATGTGAGCGTTGGCAAATTTGATGAACTGCAAATGTTCTACTACTTCATTGAGTCAGAGAGGAGTCCGGAAAACGATCCTCTGCTTCTTTGGATAACAGGAGGCCCTCGATGTTCTTCTTTTTATGGCCTCGTTTATGAAATTGGTCCAATATCTTTCCAATTTGGAGACTTGAACAAGGACCCATTAACACTTGTTACGAATCCATACGCATGGACCAAGCTAGCAAACATAATATTTTTAGATGCCCCTGCTGGTACTGGATTTTCATACTCAACAACTCTGGAAGGGTATATCAGTAGTGATACCCTGCATGCCACAAGAGCTTACGAGTTTCTCAAAAGGTGGCTTGTGACTCATCCCCAGTTTCTTGCAAATCCTCTCTACATTTCTGGAGATTCCTTCTCAGGAAAAATTGTTCCCATCATTGTTCAGCATATAACCGATGATATTGAAGCAGGAACCGAGCCACCTTTGAATCTCAAAGGATACATAATTGGCAATCCAGCTACAAACATTAAGGGGGATTTTAACTCGAGAATTGAGTATGCTCATCGTATGGCGCTTATATCAGATAGAATATACGAGTCAACTACAAGGAATTGCAAAGGCGAATACGTAGATGTAGATCCAAACAATCAACTATGCCTCAACAGTCTTCAAGCTTTCCAGGAGTGCACTGGAAGACTTGATTCCGAACACATATTAGCACCTTTGTGCCCCAGGGCAAACGACTATGACGAATTAACAGTGTTAGAAAAAAACTGGAATTGGAAGGCGGGTATCTCACTCCCTGTTGAGGATACTTTGAAAGATTTCCCCTCTTCTCTGCAGTTGTGTCGGGAAGATAGGATCCGCTACTCTGTTCTTTGGGCAAATGATATAAATGTCCGTAAAGCTCTCAACATTCGAGAGGTATATGGAACAAAAGGTGAATGGGCTAAATGCAATAGAACAACACCTTATACATTTGATATCCCAAGTAGTCTTGAATATCATCGAAACCTCAGCAAAAAGTCCCTTCGTGCTTTGGTCTACAGTGGTGATCATGATATGGCCATACCATATATAAGTACACTGGCGTGGATAGAATCCTTGAACTTGACACTTGAAGATGATTGGAAACCATGGTTTTCTGATGATCAAGTTGCCGGATACACAATTTACTATTCGAATAAAGAGTATAATTTGATATATGCCACCATAAAGGGAGGAGGGCATACAGCTCCAGAGTACAATCCTAAGGAGTGTTTTGATATGTTCAATAGGTGGCTAGGGCATTCTCCTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

509

Amino Acids

58.0

Weight (kDa)

5.41

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 65 - 503 8.4e-122 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1269
AccIII TCCGGA 1 cut(s) 301
AciI CCGC 2 cut(s) 1013, 1090
AclWI GGATC 4 cut(s) 305, 857, 1081, 1094
AcoI YGGCCR 1 cut(s) 1290
AcsI RAATTY 3 cut(s) 20, 116, 251
AfaI GTAC 3 cut(s) 493, 1309, 1469
AfiI CCNNNNNNNGG 1 cut(s) 1033
AjnI CCWGG 2 cut(s) 903, 956
AjuI GAANNNNNNNTTGG 2 cut(s) 18, 50
AleI CACNNNNGTG 2 cut(s) 949, 1315
AloI GAACNNNNNNTCC 2 cut(s) 636, 668
AluBI AGCT 8 cut(s) 29, 223, 457, 560, 743, 899, 1133, 1460
AluI AGCT 8 cut(s) 29, 223, 457, 560, 743, 899, 1133, 1460
Alw21I GWGCWC 1 cut(s) 914
Alw26I GTCTC 1 cut(s) 396
Alw44I GTGCAC 1 cut(s) 910
AlwI GGATC 4 cut(s) 305, 857, 1081, 1094
AlwNI CAGNNNCTG 1 cut(s) 102
Ama87I CYCGRG 1 cut(s) 769
Aor13HI TCCGGA 1 cut(s) 301
AoxI GGCC 3 cut(s) 337, 361, 1290
ApaLI GTGCAC 1 cut(s) 910
ApeKI GCWGC 2 cut(s) 145, 148
ApoI RAATTY 3 cut(s) 20, 116, 251
Asp700I GAANNNNTTC 1 cut(s) 1049
AspLEI GCGC 1 cut(s) 799
AspS9I GGNCC 4 cut(s) 338, 380, 415, 450
AsuC2I CCSGG 1 cut(s) 195
AsuHPI GGTGA 3 cut(s) 218, 1178, 1289
AsuII TTCGAA 1 cut(s) 1406
AsuNHI GCTAGC 1 cut(s) 457
AvaI CYCGRG 1 cut(s) 769
AvaII GGWCC 3 cut(s) 380, 415, 450
AxyI CCTNAGG 1 cut(s) 1476
BaeGI GKGCMC 2 cut(s) 914, 956
BalI TGGCCA 1 cut(s) 1292
BamHI GGATCC 1 cut(s) 1086
BbsI GAAGAC 2 cut(s) 884, 925
Bbv12I GWGCWC 1 cut(s) 914
BbvCI CCTCAGC 1 cut(s) 1241
BbvI GCAGC 2 cut(s) 132, 135
BccI CCATC 1 cut(s) 665
BcgI CGANNNNNNTGC 4 cut(s) 156, 190, 1055, 1089
BciT130I CCWGG 2 cut(s) 905, 958
BciVI GTATCC 3 cut(s) 716, 1030, 1382
BclI TGATCA 2 cut(s) 1279, 1375
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 396
BfaI CTAG 2 cut(s) 458, 1508
BfmI CTRYAG 2 cut(s) 1064, 1270
BfoI RGCGCY 1 cut(s) 800
BfuI GTATCC 3 cut(s) 716, 1030, 1382
BisI GCNGC 2 cut(s) 146, 149
BlsI GCNGC 2 cut(s) 147, 150
Bme1390I CCNGG 3 cut(s) 195, 905, 958
Bme18I GGWCC 3 cut(s) 380, 415, 450
BmeT110I CYCGRG 1 cut(s) 769
BmgT120I GGNCC 4 cut(s) 338, 380, 415, 450
BmiI GGNNCC 3 cut(s) 417, 697, 1088
BmrFI CCNGG 3 cut(s) 195, 905, 958
BmrI ACTGGG 1 cut(s) 592
BmsI GCATC 4 cut(s) 108, 143, 176, 469
BmtI GCTAGC 1 cut(s) 461
BmuI ACTGGG 1 cut(s) 592
BpiI GAAGAC 2 cut(s) 884, 925
BpmI CTGGAG 2 cut(s) 648, 1446
Bpu10I CCTNAGC 1 cut(s) 1241
Bpu14I TTCGAA 1 cut(s) 1406
BpuMI CCSGG 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 853
BsaBI GATNNNNATC 1 cut(s) 861
BsaJI CCNNGG 3 cut(s) 956, 957, 1361
BsaWI WCCGGW 2 cut(s) 203, 301
Bsc4I CCNNNNNNNGG 1 cut(s) 1033
Bse1I ACTGG 6 cut(s) 47, 499, 598, 919, 1004, 1317
Bse21I CCTNAGG 1 cut(s) 1476
Bse8I GATNNNNATC 1 cut(s) 861
BseAI TCCGGA 1 cut(s) 301
BseBI CCWGG 2 cut(s) 905, 958
BseDI CCNNGG 3 cut(s) 956, 957, 1361
BseGI GGATG 1 cut(s) 592
BseJI GATNNNNATC 1 cut(s) 861
BseLI CCNNNNNNNGG 1 cut(s) 1033
BseMII CTCAG 2 cut(s) 654, 1255
BseNI ACTGG 6 cut(s) 47, 499, 598, 919, 1004, 1317
BseRI GAGGAG 2 cut(s) 310, 1461
BseSI GKGCMC 2 cut(s) 914, 956
BseXI GCAGC 2 cut(s) 132, 135
BsgI GTGCAG 1 cut(s) 88
BshFI GGCC 3 cut(s) 339, 363, 1292
BsiHKAI GWGCWC 1 cut(s) 914
BsiHKCI CYCGRG 1 cut(s) 769
BsiSI CCGG 4 cut(s) 194, 204, 302, 1386
BslFI GGGAC 1 cut(s) 1237
BslI CCNNNNNNNGG 1 cut(s) 1033
BsmAI GTCTC 1 cut(s) 396
BsmFI GGGAC 1 cut(s) 1237
BsmI GAATGC 1 cut(s) 1513
BsnI GGCC 3 cut(s) 339, 363, 1292
BsoBI CYCGRG 1 cut(s) 769
Bsp119I TTCGAA 1 cut(s) 1406
Bsp1286I GDGCHC 2 cut(s) 914, 956
Bsp13I TCCGGA 1 cut(s) 301
Bsp143I GATC 6 cut(s) 177, 310, 862, 1086, 1279, 1375
Bsp19I CCATGG 1 cut(s) 1361
BspACI CCGC 2 cut(s) 1013, 1090
BspANI GGCC 3 cut(s) 339, 363, 1292
BspCNI CTCAG 2 cut(s) 653, 1254
BspEI TCCGGA 1 cut(s) 301
BspHI TCATGA 1 cut(s) 1282
BspLI GGNNCC 3 cut(s) 417, 697, 1088
BspMAI CTGCAG 1 cut(s) 1068
BspOI GCTAGC 1 cut(s) 461
BspPI GGATC 4 cut(s) 305, 857, 1081, 1094
BspT104I TTCGAA 1 cut(s) 1406
BsrI ACTGG 6 cut(s) 47, 499, 598, 919, 1004, 1317
BssECI CCNNGG 3 cut(s) 956, 957, 1361
BssMI GATC 6 cut(s) 177, 310, 862, 1086, 1279, 1375
BssT1I CCWWGG 1 cut(s) 1361
Bst2UI CCWGG 2 cut(s) 905, 958
Bst4CI ACNGT 4 cut(s) 174, 890, 985, 1274
Bst6I CTCTTC 1 cut(s) 1063
BstBAI YACGTR 1 cut(s) 853
BstBI TTCGAA 1 cut(s) 1406
BstC8I GCNNGC 3 cut(s) 132, 459, 549
BstDEI CTNAG 3 cut(s) 640, 1241, 1476
BstDSI CCRYGG 1 cut(s) 1361
BstEII GGTNACC 1 cut(s) 206
BstF5I GGATG 1 cut(s) 592
BstH2I RGCGCY 1 cut(s) 800
BstHHI GCGC 1 cut(s) 799
BstKTI GATC 6 cut(s) 180, 313, 865, 1089, 1282, 1378
BstMAI GTCTC 1 cut(s) 396
BstMBI GATC 6 cut(s) 177, 310, 862, 1086, 1279, 1375
BstMWI GCNNNNNNNGC 2 cut(s) 131, 557
BstNI CCWGG 2 cut(s) 905, 958
BstNSI RCATGY 1 cut(s) 551
BstPI GGTNACC 1 cut(s) 206
BstSCI CCNGG 3 cut(s) 193, 903, 956
BstSFI CTRYAG 2 cut(s) 1064, 1270
BstSLI GKGCMC 2 cut(s) 914, 956
BstSNI TACGTA 1 cut(s) 853
BstV1I GCAGC 2 cut(s) 132, 135
BstV2I GAAGAC 2 cut(s) 884, 925
BstX2I RGATCY 2 cut(s) 862, 1086
BstXI CCANNNNNNTGG 1 cut(s) 447
BstYI RGATCY 2 cut(s) 862, 1086
Bsu36I CCTNAGG 1 cut(s) 1476
BsuI GTATCC 3 cut(s) 716, 1030, 1382
BsuRI GGCC 3 cut(s) 339, 363, 1292
BtgI CCRYGG 1 cut(s) 1361
BtsCI GGATG 1 cut(s) 592
BtsIMutI CAGTG 4 cut(s) 912, 990, 1279, 1310
Cac8I GCNNGC 3 cut(s) 132, 459, 549
CaiI CAGNNNCTG 1 cut(s) 102
CciI TCATGA 1 cut(s) 1282
CfoI GCGC 1 cut(s) 799
Cfr13I GGNCC 4 cut(s) 338, 380, 415, 450
Csp6I GTAC 3 cut(s) 492, 1308, 1468
CviAII CATG 4 cut(s) 447, 548, 1283, 1362
CviQI GTAC 3 cut(s) 492, 1308, 1468
DdeI CTNAG 3 cut(s) 640, 1241, 1476
DpnI GATC 6 cut(s) 179, 312, 864, 1088, 1281, 1377
DpnII GATC 6 cut(s) 177, 310, 862, 1086, 1279, 1375
EaeI YGGCCR 1 cut(s) 1290
Eam1104I CTCTTC 1 cut(s) 1063
EarI CTCTTC 1 cut(s) 1063
Eco105I TACGTA 1 cut(s) 853
Eco130I CCWWGG 1 cut(s) 1361
Eco32I GATATC 1 cut(s) 1210
Eco47I GGWCC 3 cut(s) 380, 415, 450
Eco81I CCTNAGG 1 cut(s) 1476
Eco88I CYCGRG 1 cut(s) 769
Eco91I GGTNACC 1 cut(s) 206
EcoO109I RGGNCCY 2 cut(s) 338, 415
EcoO65I GGTNACC 1 cut(s) 206
EcoRII CCWGG 2 cut(s) 903, 956
EcoRV GATATC 1 cut(s) 1210
EcoT14I CCWWGG 1 cut(s) 1361
ErhI CCWWGG 1 cut(s) 1361
FaeI CATG 4 cut(s) 450, 551, 1286, 1365
FaqI GGGAC 1 cut(s) 1237
FatI CATG 4 cut(s) 446, 547, 1282, 1361
FauI CCCGC 1 cut(s) 1006
FbaI TGATCA 2 cut(s) 1279, 1375
FblI GTMKAC 1 cut(s) 1269
Fnu4HI GCNGC 2 cut(s) 146, 149
FokI GGATG 1 cut(s) 579
Fsp4HI GCNGC 2 cut(s) 146, 149
FspBI CTAG 2 cut(s) 458, 1508
GlaI GCGC 1 cut(s) 798
GluI GCNGC 2 cut(s) 146, 149
GsuI CTGGAG 2 cut(s) 648, 1446
HaeII RGCGCY 1 cut(s) 800
HaeIII GGCC 3 cut(s) 339, 363, 1292
HapII CCGG 4 cut(s) 194, 204, 302, 1386
HhaI GCGC 1 cut(s) 799
Hin1II CATG 4 cut(s) 450, 551, 1286, 1365
Hin6I GCGC 1 cut(s) 797
HinP1I GCGC 1 cut(s) 797
HincII GTYRAC 1 cut(s) 825
HindII GTYRAC 1 cut(s) 825
HindIII AAGCTT 3 cut(s) 27, 221, 897
HpaII CCGG 4 cut(s) 194, 204, 302, 1386
HphI GGTGA 3 cut(s) 218, 1178, 1289
Hpy166II GTNNAC 4 cut(s) 825, 912, 1270, 1310
Hpy188I TCNGA 4 cut(s) 292, 808, 931, 1372
Hpy8I GTNNAC 4 cut(s) 825, 912, 1270, 1310
HpyAV CCTTC 5 cut(s) 226, 515, 646, 1003, 1265
HpyCH4III ACNGT 4 cut(s) 174, 890, 985, 1274
HpyCH4IV ACGT 2 cut(s) 86, 852
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 557
HpyF3I CTNAG 3 cut(s) 640, 1241, 1476
HpySE526I ACGT 2 cut(s) 86, 852
Hsp92II CATG 4 cut(s) 450, 551, 1286, 1365
HspAI GCGC 1 cut(s) 797
Kpn2I TCCGGA 1 cut(s) 301
Ksp22I TGATCA 2 cut(s) 1279, 1375
Kzo9I GATC 6 cut(s) 177, 310, 862, 1086, 1279, 1375
LmnI GCTCC 1 cut(s) 1465
Lsp1109I GCAGC 2 cut(s) 132, 135
LweI GCATC 4 cut(s) 108, 143, 176, 469
MaeI CTAG 2 cut(s) 458, 1508
MaeII ACGT 2 cut(s) 86, 852
MaeIII GTNAC 3 cut(s) 206, 430, 586
MalI GATC 6 cut(s) 179, 312, 864, 1088, 1281, 1377
MboI GATC 6 cut(s) 177, 310, 862, 1086, 1279, 1375
MboII GAAGA 7 cut(s) 83, 342, 884, 930, 1050, 1091, 1358
MflI RGATCY 2 cut(s) 862, 1086
MhlI GDGCHC 2 cut(s) 914, 956
MlsI TGGCCA 1 cut(s) 1292
MluNI TGGCCA 1 cut(s) 1292
MlyI GAGTC 5 cut(s) 24, 296, 307, 583, 830
Mox20I TGGCCA 1 cut(s) 1292
MroI TCCGGA 1 cut(s) 301
MroXI GAANNNNTTC 1 cut(s) 1049
MscI TGGCCA 1 cut(s) 1292
MseI TTAA 5 cut(s) 422, 753, 765, 980, 1525
MslI CAYNNNNRTG 3 cut(s) 445, 949, 1315
Msp20I TGGCCA 1 cut(s) 1292
MspI CCGG 4 cut(s) 194, 204, 302, 1386
MspR9I CCNGG 3 cut(s) 195, 905, 958
Mva1269I GAATGC 1 cut(s) 1513
MvaI CCWGG 2 cut(s) 905, 958
MwoI GCNNNNNNNGC 2 cut(s) 131, 557
NciI CCSGG 1 cut(s) 195
NcoI CCATGG 1 cut(s) 1361
NdeII GATC 6 cut(s) 177, 310, 862, 1086, 1279, 1375
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 4 cut(s) 450, 551, 1286, 1365
NlaIV GGNNCC 3 cut(s) 417, 697, 1088
NmuCI GTSAC 2 cut(s) 206, 586
NspI RCATGY 1 cut(s) 551
NspV TTCGAA 1 cut(s) 1406
OliI CACNNNNGTG 2 cut(s) 949, 1315
PaeI GCATGC 1 cut(s) 551
PaeR7I CTCGAG 1 cut(s) 769
PagI TCATGA 1 cut(s) 1282
PasI CCCWGGG 1 cut(s) 957
PcsI WCGNNNNNNNCGW 1 cut(s) 972
PctI GAATGC 1 cut(s) 1513
PdmI GAANNNNTTC 1 cut(s) 1049
PfeI GAWTC 5 cut(s) 436, 632, 712, 926, 1325
PfoI TCCNGGA 1 cut(s) 903
PkrI GCNGC 2 cut(s) 147, 150
PleI GAGTC 5 cut(s) 23, 295, 306, 583, 829
PpsI GAGTC 5 cut(s) 23, 295, 306, 583, 829
Ppu21I YACGTR 1 cut(s) 853
PpuMI RGGWCCY 1 cut(s) 415
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 2 cut(s) 903, 956
PspEI GGTNACC 1 cut(s) 206
PspGI CCWGG 2 cut(s) 903, 956
PspN4I GGNNCC 3 cut(s) 417, 697, 1088
PspPI GGNCC 4 cut(s) 338, 380, 415, 450
PspPPI RGGWCCY 1 cut(s) 415
PstI CTGCAG 1 cut(s) 1068
PstNI CAGNNNCTG 1 cut(s) 102
PsuI RGATCY 2 cut(s) 862, 1086
RsaI GTAC 3 cut(s) 493, 1309, 1469
RsaNI GTAC 3 cut(s) 492, 1308, 1468
RseI CAYNNNNRTG 3 cut(s) 445, 949, 1315
SaqAI TTAA 5 cut(s) 422, 753, 765, 980, 1525
SatI GCNGC 2 cut(s) 146, 149
Sau3AI GATC 6 cut(s) 177, 310, 862, 1086, 1279, 1375
Sau96I GGNCC 4 cut(s) 338, 380, 415, 450
SchI GAGTC 5 cut(s) 24, 296, 307, 583, 830
ScrFI CCNGG 3 cut(s) 195, 905, 958
SduI GDGCHC 2 cut(s) 914, 956
SfaNI GCATC 4 cut(s) 108, 143, 176, 469
SfcI CTRYAG 2 cut(s) 1064, 1270
Sfr274I CTCGAG 1 cut(s) 769
SfuI TTCGAA 1 cut(s) 1406
SinI GGWCC 3 cut(s) 380, 415, 450
SlaI CTCGAG 1 cut(s) 769
SmiMI CAYNNNNRTG 3 cut(s) 445, 949, 1315
SmlI CTYRAG 1 cut(s) 769
SmoI CTYRAG 1 cut(s) 769
SnaBI TACGTA 1 cut(s) 853
SphI GCATGC 1 cut(s) 551
SsiI CCGC 2 cut(s) 1013, 1090
SspI AATATT 1 cut(s) 471
SspMI CTAG 2 cut(s) 458, 1508
StyD4I CCNGG 3 cut(s) 193, 903, 956
StyI CCWWGG 1 cut(s) 1361
TaaI ACNGT 4 cut(s) 174, 890, 985, 1274
TaiI ACGT 2 cut(s) 89, 855
TaqI TCGA 6 cut(s) 176, 343, 770, 1144, 1234, 1406
TatI WGTACW 2 cut(s) 1307, 1467
TfiI GAWTC 5 cut(s) 436, 632, 712, 926, 1325
Tru1I TTAA 5 cut(s) 422, 753, 765, 980, 1525
Tru9I TTAA 5 cut(s) 422, 753, 765, 980, 1525
TscAI CASTG 4 cut(s) 919, 990, 1279, 1317
TseFI GTSAC 2 cut(s) 206, 586
TseI GCWGC 2 cut(s) 145, 148
Tsp45I GTSAC 2 cut(s) 206, 586
TspDTI ATGAA 5 cut(s) 26, 271, 273, 387, 492
TspGWI ACGGA 1 cut(s) 1115
TspRI CASTG 4 cut(s) 919, 990, 1279, 1317
VneI GTGCAC 1 cut(s) 910
VpaK11BI GGWCC 3 cut(s) 380, 415, 450
XapI RAATTY 3 cut(s) 20, 116, 251
XceI RCATGY 1 cut(s) 551
XcmI CCANNNNNNNNNTGG 1 cut(s) 911
XhoI CTCGAG 1 cut(s) 769
XmiI GTMKAC 1 cut(s) 1269
XmnI GAANNNNTTC 1 cut(s) 1049
XspI CTAG 2 cut(s) 458, 1508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.