Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0009133

Physical Location & Seq

Genomic Coordinates Forward (+)

14582480 .. 14584831

Gene Structure 2352 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g11110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1401 bp

ATGCGGAAAATCATAAACTCCAATCTCAACCGTCGGATTAAATCCTCATCCCTTACCTTACCCTATACATTCGTAACCTCTTCACTGCATCTCCTCTACGCAACCCCTAAACTCACTTCGTGCAAGAGAAAAAAGGAGCGACCATTTTCAGTTTTCCACAGAGCTACCACGGCAACCATGGCGGCCACCGTGTCTCCGTGGGCCAAGCCCGGCGCCTGGGCCCTCGCCGCCGAGGAGCAGGAAGCCGAACTCGAACAGCAAGCCAAAGAGGAACAACAGCGCGCCATTGAGCCGCCCTCCGCTGACTTTCCATCTCTCTCAGCAGCCGCCGCCACCAAGCCCAAGAAGAAGAAACAGACCACCTCCCTCGCCGTGGAGCATCCCGTGGGCCTGACCCACGAGGACCGGATGCTTTTGCCGACCGGTCCGCGCGAGCGAACCGCCGAGGAGCTTCAACGGAATGGGCTCGGCGGCGGGTTCAAGACTTATGGGGGCGATCGGGGAAACAGTAGGTATTCCAACGGTGACGAGTCGTCGGATTCGAAGTGGGGTCAGAGGAAGGAAGGTGGGTTTGGGAAGGAATCGAATCGAGATGAACCTTCGAGGGCGGATGAGGTTGATGATTGGGGCGCCGCGAAGAAATCGATGTCGGGAAATTCCTTTGAGAGGAGGGAGAGAGGAGGTGGAGGTAGTTTTTTGGGTGGGTCACAGTCGAAGGCTGATGAATCCGACAGCTGGGTGTCGAATAAGAGCTCCATGCCGTCGGAGGGACGGAGAGTTGGCGGTGGTGGTGGCGGGTTTGATCGGGAGAGGAAAGTAGGGTTCCCGTCTAATGGAGGTGCTGATTCTGATAATTGGGGGAGGAAGAAGGAGGAGACTAATGGTGGGTCTGGATTTGATCGAGAGAGAAAGGTTGGGTACGTGTCCAGTGGCGGTGGTGCGGATTCTGATGTGTGGGGGAAGAAGAGGGGGGAGAGTAATTTTGGTCTTAATGAGAGTACTGCGAGGCCGAGGCTCAATTTGCAGCCTAGGACTTTGCCTGTGAGCAACGAGACCTCACCAGGGTCGGCCACTGCACCGAAGCCTAAGGGCTCGAACCCATTTGGTGCAGCAAGGCCGAGGGAGGAGGTGTTGGCGGAGAAAGGGAAGGACTGGAAGGAGATTGATGAGCAGCTTGAGTCTGCTAAGATTAAGGAGGTGGCGGGGCTAGCTAACAGCGAGTCGTTCGGGAGGAGGAGTTTTGGGATGGGAAATGAGCGTGCTGGTGATCGAACTGAGAGAGCTTGGAGGAAGCCTGATTCACGCCCCCAGAGTGCTGATAAAAGTGAGACTAAGAGCAACTCTGAGGAACCAGAGGGCGACCATGTTGAACCAGAGAACGAGCATGTTGATGAGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046983 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

eIF-4B

Protein Analysis

467

Amino Acids

50.7

Weight (kDa)

8.82

Isoelectric Point (pI)

48.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
eIF-4B	PF06273	66 - 263	9.9e-35	Plant specific eukaryotic initiation factor 4B
eIF-4B	PF06273	259 - 398	1.8e-14	Plant specific eukaryotic initiation factor 4B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38710 AT4G38710
fragaria_vesca	FvH4_2g01750
malus_domestica	MD05G1116800.v1.1 MD10G1119900.v1.1
prunus_persica	Prupe.8G161300_v2.0.a1
pyrus_communis	pycom05g11110 pycom10g10330
rosa_chinensis	RchiOBHm_Chr6g0244781
rosa_laevigata	RLG00000015329
rosa_multiflora	Rmu_co8397245.1_g000001 Rmu_sc0002064.1_g000007 Rmu_sc0012997.1_g000002
rosa_roxburghii	Rroxscaffold_7G00215500
rosa_rugosa	Rorug05G0510600.1 Rorug05G0510700 Rorug05G0510700
rosa_samantha	Rh6AG025800 Rh6BG021500 Rh6CG020000 Rh6DG020600
rosa_wichuraiana	Rw6G002180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	212, 629
AccII	CGCG	4 cut(s)	282, 430, 432, 635
AcoI	YGGCCR	2 cut(s)	183, 1068
AcsI	RAATTY	1 cut(s)	655
AcyI	GRCGYC	2 cut(s)	213, 630
AdeI	CACNNNGTG	1 cut(s)	120
AfaI	GTAC	2 cut(s)	920, 1000
AfiI	CCNNNNNNNGG	7 cut(s)	216, 373, 666, 735, 767, 833, 1062
AflIII	ACRYGT	1 cut(s)	921
AgeI	ACCGGT	1 cut(s)	422
AgsI	TTSAA	3 cut(s)	455, 481, 1370
AhdI	GACNNNNNGTC	1 cut(s)	532
AjnI	CCWGG	2 cut(s)	215, 1060
AjuI	GAANNNNNNNTTGG	2 cut(s)	555, 587
AluBI	AGCT	7 cut(s)	164, 451, 735, 753, 1174, 1211, 1283
AluI	AGCT	7 cut(s)	164, 451, 735, 753, 1174, 1211, 1283
Alw21I	GWGCWC	1 cut(s)	755
Alw26I	GTCTC	4 cut(s)	198, 869, 1046, 1322
AoxI	GGCC	7 cut(s)	183, 201, 219, 388, 1007, 1068, 1115
ApaI	GGGCCC	1 cut(s)	223
ApeKI	GCWGC	4 cut(s)	323, 1024, 1109, 1171
ApoI	RAATTY	1 cut(s)	655
AsiGI	ACCGGT	1 cut(s)	422
AspA2I	CCTAGG	1 cut(s)	1028
AspLEI	GCGC	5 cut(s)	215, 282, 284, 432, 632
AspS9I	GGNCC	6 cut(s)	201, 219, 220, 388, 403, 425
AsuC2I	CCSGG	1 cut(s)	210
AsuHPI	GGTGA	3 cut(s)	536, 1050, 1277
AsuII	TTCGAA	1 cut(s)	542
AsuNHI	GCTAGC	1 cut(s)	1207
AvaII	GGWCC	2 cut(s)	403, 425
AvrII	CCTAGG	1 cut(s)	1028
AxyI	CCTNAGG	1 cut(s)	1086
BaeGI	GKGCMC	1 cut(s)	223
BanI	GGYRCC	2 cut(s)	212, 629
BanII	GRGCYC	4 cut(s)	223, 468, 755, 1094
BauI	CACGAG	1 cut(s)	398
Bbv12I	GWGCWC	1 cut(s)	755
BbvI	GCAGC	4 cut(s)	335, 1036, 1121, 1183
BccI	CCATC	2 cut(s)	319, 1240
BceAI	ACGGC	3 cut(s)	186, 356, 745
BciT130I	CCWGG	2 cut(s)	217, 1062
BcnI	CCSGG	1 cut(s)	210
BcoDI	GTCTC	4 cut(s)	198, 869, 1046, 1322
BfaI	CTAG	2 cut(s)	1029, 1208
BfoI	RGCGCY	2 cut(s)	216, 633
BlnI	CCTAGG	1 cut(s)	1028
BmcAI	AGTACT	1 cut(s)	1000
Bme1390I	CCNGG	3 cut(s)	210, 217, 1062
Bme18I	GGWCC	2 cut(s)	403, 425
BmeRI	GACNNNNNGTC	1 cut(s)	532
BmgT120I	GGNCC	6 cut(s)	201, 219, 220, 388, 403, 425
BmiI	GGNNCC	5 cut(s)	214, 221, 631, 824, 1350
BmrFI	CCNGG	3 cut(s)	210, 217, 1062
BmsI	GCATC	3 cut(s)	97, 388, 399
BmtI	GCTAGC	1 cut(s)	1211
BplI	GAGNNNNNCTC	2 cut(s)	281, 313
Bpu14I	TTCGAA	1 cut(s)	542
BpuEI	CTTGAG	1 cut(s)	1196
BpuMI	CCSGG	1 cut(s)	210
Bsa29I	ATCGAT	1 cut(s)	644
BsaAI	YACGTR	1 cut(s)	922
BsaHI	GRCGYC	2 cut(s)	213, 630
BsaI	GGTCTC	1 cut(s)	1046
BsaWI	WCCGGW	2 cut(s)	405, 422
BsaXI	ACNNNNNCTCC	8 cut(s)	75, 105, 178, 208, 1115, 1145, 1222, 1252
Bsc4I	CCNNNNNNNGG	7 cut(s)	216, 373, 666, 735, 767, 833, 1062
Bse118I	RCCGGY	1 cut(s)	422
Bse1I	ACTGG	2 cut(s)	927, 1157
Bse21I	CCTNAGG	1 cut(s)	1086
BseBI	CCWGG	2 cut(s)	217, 1062
BseCI	ATCGAT	1 cut(s)	644
BseGI	GGATG	5 cut(s)	47, 379, 414, 616, 1251
BseLI	CCNNNNNNNGG	7 cut(s)	216, 373, 666, 735, 767, 833, 1062
BseMII	CTCAG	3 cut(s)	333, 1266, 1335
BseNI	ACTGG	2 cut(s)	927, 1157
BsePI	GCGCGC	1 cut(s)	280
BseRI	GAGGAG	9 cut(s)	83, 248, 461, 682, 693, 887, 1139, 1246, 1249
BseSI	GKGCMC	1 cut(s)	223
BseXI	GCAGC	4 cut(s)	335, 1036, 1121, 1183
BseYI	CCCAGC	1 cut(s)	735
BsgI	GTGCAG	2 cut(s)	1059, 1128
Bsh1236I	CGCG	4 cut(s)	282, 430, 432, 635
Bsh1285I	CGRYCG	2 cut(s)	423, 499
BshFI	GGCC	7 cut(s)	185, 203, 221, 390, 1009, 1070, 1117
BshNI	GGYRCC	2 cut(s)	212, 629
BshTI	ACCGGT	1 cut(s)	422
BshVI	ATCGAT	1 cut(s)	644
BsiEI	CGRYCG	2 cut(s)	423, 499
BsiHKAI	GWGCWC	1 cut(s)	755
BsiSI	CCGG	3 cut(s)	210, 406, 423
BslFI	GGGAC	1 cut(s)	783
BslI	CCNNNNNNNGG	7 cut(s)	216, 373, 666, 735, 767, 833, 1062
BsmAI	GTCTC	4 cut(s)	198, 869, 1046, 1322
BsmFI	GGGAC	1 cut(s)	783
BsnI	GGCC	7 cut(s)	185, 203, 221, 390, 1009, 1070, 1117
Bso31I	GGTCTC	1 cut(s)	1046
Bsp119I	TTCGAA	1 cut(s)	542
Bsp120I	GGGCCC	1 cut(s)	219
Bsp1286I	GDGCHC	4 cut(s)	223, 468, 755, 1094
Bsp143I	GATC	4 cut(s)	496, 802, 898, 1267
Bsp19I	CCATGG	1 cut(s)	177
BspANI	GGCC	7 cut(s)	185, 203, 221, 390, 1009, 1070, 1117
BspCNI	CTCAG	3 cut(s)	332, 1267, 1336
BspDI	ATCGAT	1 cut(s)	644
BspFNI	CGCG	4 cut(s)	282, 430, 432, 635
BspLI	GGNNCC	5 cut(s)	214, 221, 631, 824, 1350
BspOI	GCTAGC	1 cut(s)	1211
BspT104I	TTCGAA	1 cut(s)	542
BspT107I	GGYRCC	2 cut(s)	212, 629
BspTNI	GGTCTC	1 cut(s)	1046
BsrFI	RCCGGY	1 cut(s)	422
BsrI	ACTGG	2 cut(s)	927, 1157
BssAI	RCCGGY	1 cut(s)	422
BssHII	GCGCGC	1 cut(s)	280
BssMI	GATC	4 cut(s)	496, 802, 898, 1267
BssNI	GRCGYC	2 cut(s)	213, 630
BssSI	CACGAG	1 cut(s)	398
BssT1I	CCWWGG	2 cut(s)	177, 1028
Bst2BI	CACGAG	1 cut(s)	398
Bst2UI	CCWGG	2 cut(s)	217, 1062
Bst4CI	ACNGT	5 cut(s)	32, 190, 509, 524, 711
Bst6I	CTCTTC	2 cut(s)	85, 959
BstACI	GRCGYC	2 cut(s)	213, 630
BstBAI	YACGTR	1 cut(s)	922
BstBI	TTCGAA	1 cut(s)	542
BstC8I	GCNNGC	5 cut(s)	261, 282, 434, 1209, 1260
BstDEI	CTNAG	6 cut(s)	319, 1086, 1185, 1275, 1332, 1344
BstDSI	CCRYGG	5 cut(s)	168, 177, 197, 372, 384
BstENI	CCTNNNNNAGG	1 cut(s)	664
BstF5I	GGATG	5 cut(s)	47, 379, 414, 616, 1251
BstFNI	CGCG	4 cut(s)	282, 430, 432, 635
BstH2I	RGCGCY	2 cut(s)	216, 633
BstHHI	GCGC	5 cut(s)	215, 282, 284, 432, 632
BstKTI	GATC	4 cut(s)	499, 805, 901, 1270
BstMAI	GTCTC	4 cut(s)	198, 869, 1046, 1322
BstMBI	GATC	4 cut(s)	496, 802, 898, 1267
BstMCI	CGRYCG	2 cut(s)	423, 499
BstMWI	GCNNNNNNNGC	6 cut(s)	170, 179, 227, 329, 1021, 1208
BstNI	CCWGG	2 cut(s)	217, 1062
BstNSI	RCATGY	1 cut(s)	1388
BstSCI	CCNGG	3 cut(s)	208, 215, 1060
BstSLI	GKGCMC	1 cut(s)	223
BstUI	CGCG	4 cut(s)	282, 430, 432, 635
BstV1I	GCAGC	4 cut(s)	335, 1036, 1121, 1183
Bsu15I	ATCGAT	1 cut(s)	644
Bsu36I	CCTNAGG	1 cut(s)	1086
BsuRI	GGCC	7 cut(s)	185, 203, 221, 390, 1009, 1070, 1117
BsuTUI	ATCGAT	1 cut(s)	644
BtgI	CCRYGG	5 cut(s)	168, 177, 197, 372, 384
BtsCI	GGATG	5 cut(s)	47, 379, 414, 616, 1251
BtsI	GCAGTG	2 cut(s)	83, 1071
BtsIMutI	CAGTG	3 cut(s)	83, 934, 1071
Cac8I	GCNNGC	5 cut(s)	261, 282, 434, 1209, 1260
CfoI	GCGC	5 cut(s)	215, 282, 284, 432, 632
Cfr10I	RCCGGY	1 cut(s)	422
Cfr13I	GGNCC	6 cut(s)	201, 219, 220, 388, 403, 425
ClaI	ATCGAT	1 cut(s)	644
CpoI	CGGWCCG	1 cut(s)	425
Csp6I	GTAC	2 cut(s)	919, 999
CspAI	ACCGGT	1 cut(s)	422
CspI	CGGWCCG	1 cut(s)	425
CviAII	CATG	4 cut(s)	178, 757, 1364, 1385
CviQI	GTAC	2 cut(s)	919, 999
DdeI	CTNAG	6 cut(s)	319, 1086, 1185, 1275, 1332, 1344
DinI	GGCGCC	2 cut(s)	214, 631
DpnI	GATC	4 cut(s)	498, 804, 900, 1269
DpnII	GATC	4 cut(s)	496, 802, 898, 1267
DraIII	CACNNNGTG	1 cut(s)	120
DriI	GACNNNNNGTC	1 cut(s)	532
EaeI	YGGCCR	2 cut(s)	183, 1068
Eam1104I	CTCTTC	2 cut(s)	85, 959
Eam1105I	GACNNNNNGTC	1 cut(s)	532
EarI	CTCTTC	2 cut(s)	85, 959
EciI	GGCGGA	2 cut(s)	623, 1151
Ecl136II	GAGCTC	1 cut(s)	753
Eco130I	CCWWGG	2 cut(s)	177, 1028
Eco24I	GRGCYC	4 cut(s)	223, 468, 755, 1094
Eco31I	GGTCTC	1 cut(s)	1046
Eco47I	GGWCC	2 cut(s)	403, 425
Eco53kI	GAGCTC	1 cut(s)	753
Eco81I	CCTNAGG	1 cut(s)	1086
EcoICRI	GAGCTC	1 cut(s)	753
EcoNI	CCTNNNNNAGG	1 cut(s)	664
EcoO109I	RGGNCCY	1 cut(s)	220
EcoRII	CCWGG	2 cut(s)	215, 1060
EcoT14I	CCWWGG	2 cut(s)	177, 1028
EcoT38I	GRGCYC	4 cut(s)	223, 468, 755, 1094
EgeI	GGCGCC	2 cut(s)	214, 631
EheI	GGCGCC	2 cut(s)	214, 631
ErhI	CCWWGG	2 cut(s)	177, 1028
FaeI	CATG	4 cut(s)	181, 760, 1367, 1388
FaiI	YATR	7 cut(s)	14, 66, 179, 489, 758, 1365, 1386
FaqI	GGGAC	1 cut(s)	783
FatI	CATG	4 cut(s)	177, 756, 1363, 1384
FauI	CCCGC	3 cut(s)	467, 788, 1195
FokI	GGATG	5 cut(s)	34, 366, 421, 623, 1258
FriOI	GRGCYC	4 cut(s)	223, 468, 755, 1094
FspBI	CTAG	2 cut(s)	1029, 1208
GlaI	GCGC	5 cut(s)	214, 281, 283, 431, 631
GsaI	CCCAGC	1 cut(s)	739
HaeII	RGCGCY	2 cut(s)	216, 633
HaeIII	GGCC	7 cut(s)	185, 203, 221, 390, 1009, 1070, 1117
HapII	CCGG	3 cut(s)	210, 406, 423
HhaI	GCGC	5 cut(s)	215, 282, 284, 432, 632
Hin1I	GRCGYC	2 cut(s)	213, 630
Hin1II	CATG	4 cut(s)	181, 760, 1367, 1388
Hin6I	GCGC	5 cut(s)	213, 280, 282, 430, 630
HinP1I	GCGC	5 cut(s)	213, 280, 282, 430, 630
HpaII	CCGG	3 cut(s)	210, 406, 423
HphI	GGTGA	3 cut(s)	536, 1050, 1277
Hpy188I	TCNGA	8 cut(s)	36, 538, 555, 730, 766, 850, 949, 1345
Hpy188III	TCNNGA	7 cut(s)	481, 590, 651, 806, 891, 902, 1228
Hpy99I	CGWCG	3 cut(s)	36, 538, 766
HpyAV	CCTTC	8 cut(s)	553, 557, 571, 609, 709, 862, 1141, 1150
HpyCH4III	ACNGT	5 cut(s)	32, 190, 509, 524, 711
HpyCH4IV	ACGT	1 cut(s)	921
HpyCH4V	TGCA	5 cut(s)	88, 123, 1024, 1076, 1109
HpyF10VI	GCNNNNNNNGC	6 cut(s)	170, 179, 227, 329, 1021, 1208
HpyF3I	CTNAG	6 cut(s)	319, 1086, 1185, 1275, 1332, 1344
HpySE526I	ACGT	1 cut(s)	921
Hsp92I	GRCGYC	2 cut(s)	213, 630
Hsp92II	CATG	4 cut(s)	181, 760, 1367, 1388
HspAI	GCGC	5 cut(s)	213, 280, 282, 430, 630
KasI	GGCGCC	2 cut(s)	212, 629
Kzo9I	GATC	4 cut(s)	496, 802, 898, 1267
LmnI	GCTCC	5 cut(s)	136, 235, 376, 448, 758
Lsp1109I	GCAGC	4 cut(s)	335, 1036, 1121, 1183
LweI	GCATC	3 cut(s)	97, 388, 399
MaeI	CTAG	2 cut(s)	1029, 1208
MaeII	ACGT	1 cut(s)	921
MaeIII	GTNAC	3 cut(s)	73, 524, 705
MalI	GATC	4 cut(s)	498, 804, 900, 1269
MboI	GATC	4 cut(s)	496, 802, 898, 1267
MboII	GAAGA	7 cut(s)	72, 358, 361, 649, 877, 973, 976
MhlI	GDGCHC	4 cut(s)	223, 468, 755, 1094
MluCI	AATT	5 cut(s)	655, 853, 979, 1018, 1396
Mly113I	GGCGCC	2 cut(s)	213, 630
MlyI	GAGTC	3 cut(s)	539, 1187, 1229
MmeI	TCCRAC	5 cut(s)	14, 516, 543, 744, 753
MseI	TTAA	3 cut(s)	39, 990, 1191
MslI	CAYNNNNRTG	1 cut(s)	1389
MspA1I	CMGCKG	2 cut(s)	302, 735
MspI	CCGG	3 cut(s)	210, 406, 423
MspR9I	CCNGG	3 cut(s)	210, 217, 1062
MvaI	CCWGG	2 cut(s)	217, 1062
MvnI	CGCG	4 cut(s)	282, 430, 432, 635
MwoI	GCNNNNNNNGC	6 cut(s)	170, 179, 227, 329, 1021, 1208
NarI	GGCGCC	2 cut(s)	213, 630
NciI	CCSGG	1 cut(s)	210
NcoI	CCATGG	1 cut(s)	177
NdeII	GATC	4 cut(s)	496, 802, 898, 1267
NheI	GCTAGC	1 cut(s)	1207
NlaIII	CATG	4 cut(s)	181, 760, 1367, 1388
NlaIV	GGNNCC	5 cut(s)	214, 221, 631, 824, 1350
NmeAIII	GCCGAG	5 cut(s)	256, 447, 469, 1035, 1143
NmuCI	GTSAC	2 cut(s)	524, 705
NspI	RCATGY	1 cut(s)	1388
NspV	TTCGAA	1 cut(s)	542
PauI	GCGCGC	1 cut(s)	280
PcsI	WCGNNNNNNNCGW	1 cut(s)	539
PfeI	GAWTC	7 cut(s)	539, 581, 586, 725, 845, 944, 1298
PinAI	ACCGGT	1 cut(s)	422
Ple19I	CGATCG	1 cut(s)	499
PleI	GAGTC	3 cut(s)	538, 1186, 1228
PluTI	GGCGCC	2 cut(s)	216, 633
PpsI	GAGTC	3 cut(s)	538, 1186, 1228
Ppu21I	YACGTR	1 cut(s)	922
Psp124BI	GAGCTC	1 cut(s)	755
Psp6I	CCWGG	2 cut(s)	215, 1060
PspFI	CCCAGC	1 cut(s)	735
PspGI	CCWGG	2 cut(s)	215, 1060
PspN4I	GGNNCC	5 cut(s)	214, 221, 631, 824, 1350
PspOMI	GGGCCC	1 cut(s)	219
PspPI	GGNCC	6 cut(s)	201, 219, 220, 388, 403, 425
PteI	GCGCGC	1 cut(s)	280
PvuI	CGATCG	1 cut(s)	499
PvuII	CAGCTG	1 cut(s)	735
RsaI	GTAC	2 cut(s)	920, 1000
RsaNI	GTAC	2 cut(s)	919, 999
RseI	CAYNNNNRTG	1 cut(s)	1389
Rsr2I	CGGWCCG	1 cut(s)	425
RsrII	CGGWCCG	1 cut(s)	425
SacI	GAGCTC	1 cut(s)	755
SaqAI	TTAA	3 cut(s)	39, 990, 1191
Sau3AI	GATC	4 cut(s)	496, 802, 898, 1267
Sau96I	GGNCC	6 cut(s)	201, 219, 220, 388, 403, 425
ScaI	AGTACT	1 cut(s)	1000
SchI	GAGTC	3 cut(s)	539, 1187, 1229
ScrFI	CCNGG	3 cut(s)	210, 217, 1062
SduI	GDGCHC	4 cut(s)	223, 468, 755, 1094
SfaNI	GCATC	3 cut(s)	97, 388, 399
SfoI	GGCGCC	2 cut(s)	214, 631
SfuI	TTCGAA	1 cut(s)	542
SinI	GGWCC	2 cut(s)	403, 425
SmiMI	CAYNNNNRTG	1 cut(s)	1389
SmlI	CTYRAG	1 cut(s)	1175
SmoI	CTYRAG	1 cut(s)	1175
Sse9I	AATT	5 cut(s)	655, 853, 979, 1018, 1396
SspDI	GGCGCC	2 cut(s)	212, 629
SspMI	CTAG	2 cut(s)	1029, 1208
SstI	GAGCTC	1 cut(s)	755
StyD4I	CCNGG	3 cut(s)	208, 215, 1060
StyI	CCWWGG	2 cut(s)	177, 1028
TaaI	ACNGT	5 cut(s)	32, 190, 509, 524, 711
TaiI	ACGT	1 cut(s)	924
TasI	AATT	5 cut(s)	655, 853, 979, 1018, 1396
TatI	WGTACW	1 cut(s)	998
TauI	GCSGC	7 cut(s)	185, 230, 295, 329, 332, 474, 635
TfiI	GAWTC	7 cut(s)	539, 581, 586, 725, 845, 944, 1298
Tru1I	TTAA	3 cut(s)	39, 990, 1191
Tru9I	TTAA	3 cut(s)	39, 990, 1191
TscAI	CASTG	3 cut(s)	90, 934, 1078
TseFI	GTSAC	2 cut(s)	524, 705
TseI	GCWGC	4 cut(s)	323, 1024, 1109, 1171
Tsp45I	GTSAC	2 cut(s)	524, 705
TspDTI	ATGAA	2 cut(s)	609, 738
TspGWI	ACGGA	3 cut(s)	186, 472, 787
TspRI	CASTG	3 cut(s)	90, 934, 1078
VpaK11BI	GGWCC	2 cut(s)	403, 425
XagI	CCTNNNNNAGG	1 cut(s)	664
XapI	RAATTY	1 cut(s)	655
XceI	RCATGY	1 cut(s)	1388
XcmI	CCANNNNNNNNNTGG	1 cut(s)	175
XmaJI	CCTAGG	1 cut(s)	1028
XspI	CTAG	2 cut(s)	1029, 1208
ZrmI	AGTACT	1 cut(s)	1000

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.