RLG00000015329

Plant specific eukaryotic initiation factor 4B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009133
Physical Location & Seq
Forward (+)
64648782 .. 64650955
2174 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015329

Sequence Viewer

Length: 1284 bp
ATGGCGGCAACAGTGTCGTCTCCTTGGGCCAAGCCCGGCGCGTGGGCCCTCGACGCCGAAGAGCACGAAGCCGAGCTGGAACAACAACAACAGCAGACCAAGATCGAAACGCAGCAGCCCCTCGCCGACTTTCCCTCTCTCTCCGCCGCCGCCGCGAAGCCGAAGAAGAAGAACAAGGGCCAGAAGGTCAGCCTAGCCGAGTTCTCCACCTTCGGCGGGCCCAAACCCGCCGAGCCCGTGGGCCTGACCCACGAGGATAGGTTGGTTCTCCCGACCGGCCCGCGCGAGCGGACAGCCGAGGAGCTCGAACGGAACCGGCCCGGCGGCGGGTTCAAGAGCTACGGCGGCGATCGGGGTAACCGCGAGGATTTGAGTTCGAAGTGGGGGAATCAGAGGAGGGAAGGAGGAGGGTTCGGGGGGAAAGAAGGAGGGGAGAGAGATGGGCCGTCGCGGGCGGATGAGGCCGACGACTGGGGCGCCGGGAAGAAATCGGTGGCGGGGAATGGGTTTGAGAAGAGGGATAGAGGAGGGTTCTTTGGGTCTCAGTCGAAGGCGGATGAATCTGACAGCTGGGTCTCGAATAAGAGCTCGTTTTCGTCGGAGGGAAGGAGATACGGCGGCTCTGGCAGTGGGTTTGAACGGGAGAGGAAGGTTGGGTTTGCATCGAATGGCGGCGGTGCTGATTCGGAGAGCTGGGGGAGGAAGAGGGAGGAGAGTAATGGTGGGGGTGGGTTTGAAAGGGAGAGGAAGGTAGGGTTAGGGTTCAATTCGAACGGTGGAGGTGCGGATTCGGAGAGTTGGGGAAGGAAGAGGGAGGAGAGTAATGGTGGGATGGAGAGTACTGGGAGGCCGAGGCTCAATTTGCAGCCGAGGACATTGCCTGTTACTCTGCCTGTTGTGAGCAATGAAACCTCTCCGGTGGCGGTGCCTGTGGATGTGGCGGCAGCACCAAGGCCCAGGGGTTCGAACCCGTTTGGGGCGGCGAGGCCGAGAGAGGAGGTTTTGGCAGAGAAAGGGCAGGATTGGAAGAAGATTGACGAGCAGCTTGAGTCTGTGAAGCTTAAGGAGAAGGAGGCTGTGGCTGCCGAAGGGGAGTCTTTTGGGAAGAGGAGTTTCGGGATGGGAAATGGGCGCTCTGGTGACCGCACCGAGGGAGCTTGGAGGAAACCTGTTGTGGCTGAGGCGGAGGCTGAGGCTCGTCCTCAGAGTGCTGGAAACGATGAGAGTAGGAGCAGCAATTCCGAGGAACCAGAACCGGAGAATGAGAATGTCAATGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046983 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-4B

Protein Analysis

428

Amino Acids

45.74

Weight (kDa)

5.46

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-4B PF06273 6 - 213 5.1e-36 Plant specific eukaryotic initiation factor 4B
eIF-4B PF06273 183 - 355 7e-16 Plant specific eukaryotic initiation factor 4B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 572
AccB1I GGYRCC 2 cut(s) 476, 925
AccBSI CCGCTC 1 cut(s) 289
AccII CGCG 6 cut(s) 41, 155, 283, 285, 363, 451
AcyI GRCGYC 2 cut(s) 54, 477
AfaI GTAC 1 cut(s) 841
AfiI CCNNNNNNNGG 7 cut(s) 42, 216, 326, 327, 471, 976, 1150
AflII CTTAAG 1 cut(s) 1061
AgsI TTSAA 4 cut(s) 334, 638, 737, 766
AjnI CCWGG 1 cut(s) 956
AluBI AGCT 9 cut(s) 76, 304, 339, 570, 588, 693, 1045, 1060, 1157
AluI AGCT 9 cut(s) 76, 304, 339, 570, 588, 693, 1045, 1060, 1157
Alw21I GWGCWC 3 cut(s) 66, 306, 590
Alw26I GTCTC 3 cut(s) 24, 546, 580
ApaI GGGCCC 2 cut(s) 49, 222
ApeKI GCWGC 7 cut(s) 112, 115, 865, 944, 1042, 1082, 1233
AspLEI GCGC 4 cut(s) 41, 285, 479, 1134
AsuC2I CCSGG 3 cut(s) 36, 321, 481
AsuHPI GGTGA 1 cut(s) 1151
AsuII TTCGAA 3 cut(s) 377, 770, 965
BaeGI GKGCMC 2 cut(s) 49, 222
BanI GGYRCC 2 cut(s) 476, 925
BanII GRGCYC 5 cut(s) 49, 222, 237, 306, 590
BauI CACGAG 1 cut(s) 251
Bbv12I GWGCWC 3 cut(s) 66, 306, 590
BbvCI CCTCAGC 2 cut(s) 1179, 1191
BbvI GCAGC 7 cut(s) 124, 127, 877, 956, 1054, 1069, 1245
BccI CCATC 3 cut(s) 434, 826, 1114
BceAI ACGGC 3 cut(s) 358, 430, 631
BcgI CGANNNNNNTGC 3 cut(s) 31, 859, 893
BciT130I CCWGG 1 cut(s) 958
BcnI CCSGG 3 cut(s) 36, 321, 481
BcoDI GTCTC 3 cut(s) 24, 546, 580
BfaI CTAG 1 cut(s) 194
BfoI RGCGCY 2 cut(s) 480, 1135
BfrI CTTAAG 1 cut(s) 1061
BmcAI AGTACT 1 cut(s) 841
Bme1390I CCNGG 4 cut(s) 36, 321, 481, 958
BmiI GGNNCC 6 cut(s) 47, 220, 314, 478, 927, 1248
BmrFI CCNGG 4 cut(s) 36, 321, 481, 958
BmrI ACTGGG 2 cut(s) 481, 852
BmsI GCATC 1 cut(s) 671
BmuI ACTGGG 2 cut(s) 481, 852
Bpu10I CCTNAGC 2 cut(s) 1179, 1191
Bpu14I TTCGAA 3 cut(s) 377, 770, 965
BpuEI CTTGAG 1 cut(s) 1067
BpuMI CCSGG 3 cut(s) 36, 321, 481
BsaHI GRCGYC 2 cut(s) 54, 477
BsaI GGTCTC 2 cut(s) 546, 580
BsaWI WCCGGW 2 cut(s) 916, 1255
Bsc4I CCNNNNNNNGG 7 cut(s) 42, 216, 326, 327, 471, 976, 1150
Bse118I RCCGGY 2 cut(s) 275, 315
Bse1I ACTGG 2 cut(s) 476, 847
Bse3DI GCAATG 2 cut(s) 875, 910
BseBI CCWGG 1 cut(s) 958
BseGI GGATG 5 cut(s) 463, 562, 837, 940, 1125
BseLI CCNNNNNNNGG 7 cut(s) 42, 216, 326, 327, 471, 976, 1150
BseMI GCAATG 2 cut(s) 875, 910
BseMII CTCAG 4 cut(s) 557, 1170, 1182, 1217
BseNI ACTGG 2 cut(s) 476, 847
BseRI GAGGAG 8 cut(s) 314, 409, 420, 540, 725, 830, 1010, 1123
BseSI GKGCMC 2 cut(s) 49, 222
BseXI GCAGC 7 cut(s) 124, 127, 877, 956, 1054, 1069, 1245
BseYI CCCAGC 2 cut(s) 570, 693
Bsh1236I CGCG 6 cut(s) 41, 155, 283, 285, 363, 451
Bsh1285I CGRYCG 2 cut(s) 276, 352
BshNI GGYRCC 2 cut(s) 476, 925
BsiEI CGRYCG 2 cut(s) 276, 352
BsiHKAI GWGCWC 3 cut(s) 66, 306, 590
BsiSI CCGG 7 cut(s) 36, 276, 316, 321, 480, 917, 1256
BslI CCNNNNNNNGG 7 cut(s) 42, 216, 326, 327, 471, 976, 1150
BsmAI GTCTC 3 cut(s) 24, 546, 580
BsmBI CGTCTC 1 cut(s) 24
Bso31I GGTCTC 2 cut(s) 546, 580
Bsp119I TTCGAA 3 cut(s) 377, 770, 965
Bsp120I GGGCCC 2 cut(s) 45, 218
Bsp1286I GDGCHC 6 cut(s) 49, 66, 222, 237, 306, 590
Bsp143I GATC 2 cut(s) 102, 349
BspCNI CTCAG 4 cut(s) 556, 1171, 1183, 1216
BspFNI CGCG 6 cut(s) 41, 155, 283, 285, 363, 451
BspLI GGNNCC 6 cut(s) 47, 220, 314, 478, 927, 1248
BspQI GCTCTTC 1 cut(s) 54
BspT104I TTCGAA 3 cut(s) 377, 770, 965
BspT107I GGYRCC 2 cut(s) 476, 925
BspTI CTTAAG 1 cut(s) 1061
BspTNI GGTCTC 2 cut(s) 546, 580
BsrBI CCGCTC 1 cut(s) 289
BsrDI GCAATG 2 cut(s) 875, 910
BsrFI RCCGGY 2 cut(s) 275, 315
BsrI ACTGG 2 cut(s) 476, 847
BssAI RCCGGY 2 cut(s) 275, 315
BssMI GATC 2 cut(s) 102, 349
BssNI GRCGYC 2 cut(s) 54, 477
BssSI CACGAG 1 cut(s) 251
BssT1I CCWWGG 2 cut(s) 23, 950
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 958
Bst4CI ACNGT 2 cut(s) 13, 776
Bst6I CTCTTC 5 cut(s) 54, 509, 698, 803, 1100
BstACI GRCGYC 2 cut(s) 54, 477
BstAFI CTTAAG 1 cut(s) 1061
BstBI TTCGAA 3 cut(s) 377, 770, 965
BstC8I GCNNGC 4 cut(s) 218, 281, 287, 453
BstDEI CTNAG 4 cut(s) 543, 1179, 1191, 1203
BstDSI CCRYGG 1 cut(s) 237
BstEII GGTNACC 2 cut(s) 356, 1139
BstF5I GGATG 5 cut(s) 463, 562, 837, 940, 1125
BstFNI CGCG 6 cut(s) 41, 155, 283, 285, 363, 451
BstH2I RGCGCY 2 cut(s) 480, 1135
BstHHI GCGC 4 cut(s) 41, 285, 479, 1134
BstKTI GATC 2 cut(s) 105, 352
BstMAI GTCTC 3 cut(s) 24, 546, 580
BstMBI GATC 2 cut(s) 102, 349
BstMCI CGRYCG 2 cut(s) 276, 352
BstMWI GCNNNNNNNGC 7 cut(s) 53, 152, 345, 461, 624, 862, 1082
BstNI CCWGG 1 cut(s) 958
BstPI GGTNACC 2 cut(s) 356, 1139
BstSCI CCNGG 4 cut(s) 34, 319, 479, 956
BstSLI GKGCMC 2 cut(s) 49, 222
BstUI CGCG 6 cut(s) 41, 155, 283, 285, 363, 451
BstV1I GCAGC 7 cut(s) 124, 127, 877, 956, 1054, 1069, 1245
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 5 cut(s) 463, 562, 837, 940, 1125
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 2 cut(s) 18, 634
Cac8I GCNNGC 4 cut(s) 218, 281, 287, 453
CfoI GCGC 4 cut(s) 41, 285, 479, 1134
Cfr10I RCCGGY 2 cut(s) 275, 315
CseI GACGC 1 cut(s) 62
Csp6I GTAC 1 cut(s) 840
CviQI GTAC 1 cut(s) 840
DdeI CTNAG 4 cut(s) 543, 1179, 1191, 1203
DinI GGCGCC 1 cut(s) 478
DpnI GATC 2 cut(s) 104, 351
DpnII GATC 2 cut(s) 102, 349
DrdI GACNNNNNNGTC 1 cut(s) 572
DseDI GACNNNNNNGTC 1 cut(s) 572
Eam1104I CTCTTC 5 cut(s) 54, 509, 698, 803, 1100
EarI CTCTTC 5 cut(s) 54, 509, 698, 803, 1100
EciI GGCGGA 4 cut(s) 133, 470, 569, 1199
Ecl136II GAGCTC 2 cut(s) 304, 588
Eco130I CCWWGG 2 cut(s) 23, 950
Eco24I GRGCYC 5 cut(s) 49, 222, 237, 306, 590
Eco31I GGTCTC 2 cut(s) 546, 580
Eco53kI GAGCTC 2 cut(s) 304, 588
Eco91I GGTNACC 2 cut(s) 356, 1139
EcoICRI GAGCTC 2 cut(s) 304, 588
EcoO109I RGGNCCY 1 cut(s) 46
EcoO65I GGTNACC 2 cut(s) 356, 1139
EcoRII CCWGG 1 cut(s) 956
EcoT14I CCWWGG 2 cut(s) 23, 950
EcoT38I GRGCYC 5 cut(s) 49, 222, 237, 306, 590
EgeI GGCGCC 1 cut(s) 478
EheI GGCGCC 1 cut(s) 478
ErhI CCWWGG 2 cut(s) 23, 950
Esp3I CGTCTC 1 cut(s) 24
FauI CCCGC 6 cut(s) 209, 235, 288, 320, 444, 490
FokI GGATG 5 cut(s) 470, 569, 844, 947, 1132
FriOI GRGCYC 5 cut(s) 49, 222, 237, 306, 590
FspBI CTAG 1 cut(s) 194
GlaI GCGC 4 cut(s) 40, 284, 478, 1133
GsaI CCCAGC 2 cut(s) 574, 697
HaeII RGCGCY 2 cut(s) 480, 1135
HapII CCGG 7 cut(s) 36, 276, 316, 321, 480, 917, 1256
HgaI GACGC 1 cut(s) 62
HhaI GCGC 4 cut(s) 41, 285, 479, 1134
Hin1I GRCGYC 2 cut(s) 54, 477
Hin6I GCGC 4 cut(s) 39, 283, 477, 1132
HinP1I GCGC 4 cut(s) 39, 283, 477, 1132
HindIII AAGCTT 1 cut(s) 1058
HinfI GANTC 6 cut(s) 388, 560, 683, 788, 1049, 1094
HpaII CCGG 7 cut(s) 36, 276, 316, 321, 480, 917, 1256
HphI GGTGA 1 cut(s) 1151
Hpy188I TCNGA 7 cut(s) 393, 565, 601, 688, 793, 1206, 1243
Hpy188III TCNNGA 4 cut(s) 271, 334, 577, 1117
Hpy99I CGWCG 4 cut(s) 56, 451, 470, 601
HpyCH4III ACNGT 2 cut(s) 13, 776
HpyCH4V TGCA 2 cut(s) 662, 865
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 152, 345, 461, 624, 862, 1082
HpyF3I CTNAG 4 cut(s) 543, 1179, 1191, 1203
Hsp92I GRCGYC 2 cut(s) 54, 477
HspAI GCGC 4 cut(s) 39, 283, 477, 1132
KasI GGCGCC 1 cut(s) 476
Kzo9I GATC 2 cut(s) 102, 349
LguI GCTCTTC 1 cut(s) 54
LmnI GCTCC 3 cut(s) 301, 1154, 1230
Lsp1109I GCAGC 7 cut(s) 124, 127, 877, 956, 1054, 1069, 1245
LweI GCATC 1 cut(s) 671
MaeI CTAG 1 cut(s) 194
MaeIII GTNAC 3 cut(s) 356, 883, 1139
MalI GATC 2 cut(s) 104, 351
MbiI CCGCTC 1 cut(s) 289
MboI GATC 2 cut(s) 102, 349
MhlI GDGCHC 6 cut(s) 49, 66, 222, 237, 306, 590
MluCI AATT 3 cut(s) 766, 859, 1237
Mly113I GGCGCC 1 cut(s) 477
MlyI GAGTC 2 cut(s) 1058, 1103
MmeI TCCRAC 1 cut(s) 579
MseI TTAA 1 cut(s) 1062
MspA1I CMGCKG 1 cut(s) 570
MspCI CTTAAG 1 cut(s) 1061
MspI CCGG 7 cut(s) 36, 276, 316, 321, 480, 917, 1256
MspR9I CCNGG 4 cut(s) 36, 321, 481, 958
MvaI CCWGG 1 cut(s) 958
MvnI CGCG 6 cut(s) 41, 155, 283, 285, 363, 451
MwoI GCNNNNNNNGC 7 cut(s) 53, 152, 345, 461, 624, 862, 1082
NarI GGCGCC 1 cut(s) 477
NciI CCSGG 3 cut(s) 36, 321, 481
NdeII GATC 2 cut(s) 102, 349
NlaIV GGNNCC 6 cut(s) 47, 220, 314, 478, 927, 1248
NmeAIII GCCGAG 7 cut(s) 97, 223, 256, 322, 876, 894, 1014
NmuCI GTSAC 1 cut(s) 1139
NspV TTCGAA 3 cut(s) 377, 770, 965
PasI CCCWGGG 1 cut(s) 957
PciSI GCTCTTC 1 cut(s) 54
PfeI GAWTC 4 cut(s) 388, 560, 683, 788
Ple19I CGATCG 1 cut(s) 352
PleI GAGTC 2 cut(s) 1057, 1102
PluTI GGCGCC 1 cut(s) 480
PpsI GAGTC 2 cut(s) 1057, 1102
Psp124BI GAGCTC 2 cut(s) 306, 590
Psp6I CCWGG 1 cut(s) 956
PspEI GGTNACC 2 cut(s) 356, 1139
PspFI CCCAGC 2 cut(s) 570, 693
PspGI CCWGG 1 cut(s) 956
PspN4I GGNNCC 6 cut(s) 47, 220, 314, 478, 927, 1248
PspOMI GGGCCC 2 cut(s) 45, 218
PvuI CGATCG 1 cut(s) 352
PvuII CAGCTG 1 cut(s) 570
RsaI GTAC 1 cut(s) 841
RsaNI GTAC 1 cut(s) 840
SacI GAGCTC 2 cut(s) 306, 590
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 1 cut(s) 1062
Sau3AI GATC 2 cut(s) 102, 349
ScaI AGTACT 1 cut(s) 841
SchI GAGTC 2 cut(s) 1058, 1103
ScrFI CCNGG 4 cut(s) 36, 321, 481, 958
SduI GDGCHC 6 cut(s) 49, 66, 222, 237, 306, 590
SfaNI GCATC 1 cut(s) 671
SfoI GGCGCC 1 cut(s) 478
SfuI TTCGAA 3 cut(s) 377, 770, 965
SmlI CTYRAG 2 cut(s) 1046, 1061
SmoI CTYRAG 2 cut(s) 1046, 1061
Sse9I AATT 3 cut(s) 766, 859, 1237
SspDI GGCGCC 1 cut(s) 476
SspMI CTAG 1 cut(s) 194
SstI GAGCTC 2 cut(s) 306, 590
StyD4I CCNGG 4 cut(s) 34, 319, 479, 956
StyI CCWWGG 2 cut(s) 23, 950
TaaI ACNGT 2 cut(s) 13, 776
TaqI TCGA 9 cut(s) 51, 105, 306, 377, 548, 578, 665, 770, 965
TasI AATT 3 cut(s) 766, 859, 1237
TatI WGTACW 1 cut(s) 839
TfiI GAWTC 4 cut(s) 388, 560, 683, 788
Tru1I TTAA 1 cut(s) 1062
Tru9I TTAA 1 cut(s) 1062
TscAI CASTG 2 cut(s) 18, 634
TseFI GTSAC 1 cut(s) 1139
TseI GCWGC 7 cut(s) 112, 115, 865, 944, 1042, 1082, 1233
Tsp45I GTSAC 1 cut(s) 1139
TspDTI ATGAA 2 cut(s) 573, 921
TspGWI ACGGA 1 cut(s) 325
TspRI CASTG 2 cut(s) 18, 634
Vha464I CTTAAG 1 cut(s) 1061
XspI CTAG 1 cut(s) 194
ZrmI AGTACT 1 cut(s) 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.