Rroxscaffold_7G00215500

Plant specific eukaryotic initiation factor 4B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009133
Physical Location & Seq
Forward (+)
66131377 .. 66133544
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215500.1

Sequence Viewer

Length: 1299 bp
ATGGCGGCAACAGTGTCGTCTCCTTGGGCCAAGCCCGGCGCGTGGGCCCTCGACGCCGAAGAGCACGAAGCCGAGCTCGAACAACAACAACAGACCAAGATCGAAACGCAGCAGCCCCTCGCCGACTTTCCCTCTCTCTCCGCCGCCGCCGCGAAGCCGAAGAAGAAGAACAAGGGCCAGAAGGTCAGCCTTGCCGAGTTCTCCACCTTCGGCGGGCCCAAGCCCGCCGAGCCCGTGGGCCTGACCCACGAGGATCGGTTGGTTCTCCCGACAGGCCCGCGCGAGCGGACCGCCGAGGAGCTCGAACGGAACCGGATTGGCGGCGGGTTCAAGAGCTACGGCGGCGATCGGGTGAACCGCGAGGATTCGAGTTCGAAGTGGGGGAGTCAGAGGAGGGAAGGAGGAGGGTTTGGGGGGAAAGAAGGAGGAGAGAGAGATGGGCCGTCGCGGGCGGATGAGGCCGACGATTGGGGCGCCGGGAAGAAATCGGCGGCGGGGAATGGGTTTGAGAAGAGGGAGAGAGGAGGGTTCTTTGGGTCCCAGTCGAAGGCGGATGAATCTGACAGCTGGGTCTCGAACAAGAGCTCGTTTTCGTCGGAGGGAAGGAGATACGGCGGCTCCGGCGGCGGGTTTGAGCGGGAGAGGAAGGTTGGGTTTGCATCGAATGGCGGCGGTGCTGATTCGGAGAGCTGGGGGAGGAAGAGGGAGGAGAGTAATGGTGGGGGTGGGTTTGAAAGGGAGAGGAAGGTAGGGTTAGGGTTCAATTCGAACGGTGGCGGTGCGGATTCGGAGAGTTGGGGAAGGAAGAGGGAGGAGAGTAATGGTGGGATGGAGAGTACTGGGAGGCCGAGGCTCAATTTGCAGCCGAGGACATTGCCTGTTACTCTGCCTGTTGTGAGCAATGAAACCTCTCCGGTGGTGGTGCCTGTGGATGTGGCGGCAGCACCAAGGCCCAGGGGTTCAAACCCGTTTGGGGCGGCGAGGCCCAGAGAGGAGGTTTTGGCAGAGAAGGGGCAGGATTGGAAGAAGATTGACGAGCAGCTTGAGTCTGTGAAACTTAAGGAGAAGGAGGCTGTGGCTGCCGAAGGGGAGTCTTTTGGGAAGAGGAGTTTCGGGATGGGAAATGGGCGCTCTGGTGACCGCACCGTGGGAGCTTGGAGGAAACCTGTTGTGGCTGAGGCGGAGGCTGAGGCTGAGGCTGAGGCTCGAGGCTCGTCTCAGAGTGCTGGAAACGATGAGAGTAAGAGCAGCAATTCTGAGGAACCAGAACCGGAGAATGAGAATGTCAATGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046983 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-4B

Protein Analysis

432

Amino Acids

46.08

Weight (kDa)

5.39

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-4B PF06273 6 - 353 4.2e-49 Plant specific eukaryotic initiation factor 4B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 569
AccB1I GGYRCC 2 cut(s) 473, 922
AccBSI CCGCTC 2 cut(s) 286, 637
AccII CGCG 6 cut(s) 41, 152, 280, 282, 360, 448
AclWI GGATC 1 cut(s) 261
AcyI GRCGYC 2 cut(s) 54, 474
AfaI GTAC 1 cut(s) 838
AfiI CCNNNNNNNGG 7 cut(s) 42, 213, 468, 547, 627, 973, 1147
AflII CTTAAG 1 cut(s) 1058
AgsI TTSAA 4 cut(s) 331, 734, 763, 963
AjnI CCWGG 1 cut(s) 953
AluBI AGCT 8 cut(s) 76, 301, 336, 567, 585, 690, 1042, 1154
AluI AGCT 8 cut(s) 76, 301, 336, 567, 585, 690, 1042, 1154
Alw21I GWGCWC 4 cut(s) 66, 78, 303, 587
Alw26I GTCTC 3 cut(s) 24, 577, 1221
AlwI GGATC 1 cut(s) 261
Ama87I CYCGRG 1 cut(s) 1206
ApaI GGGCCC 2 cut(s) 49, 219
ApeKI GCWGC 7 cut(s) 109, 112, 862, 941, 1039, 1079, 1248
AspLEI GCGC 4 cut(s) 41, 282, 476, 1131
AsuC2I CCSGG 2 cut(s) 36, 478
AsuHPI GGTGA 2 cut(s) 364, 1148
AsuII TTCGAA 2 cut(s) 374, 767
AvaI CYCGRG 1 cut(s) 1206
AvaII GGWCC 2 cut(s) 288, 537
BaeGI GKGCMC 2 cut(s) 49, 219
BanI GGYRCC 2 cut(s) 473, 922
BanII GRGCYC 6 cut(s) 49, 78, 219, 234, 303, 587
BauI CACGAG 1 cut(s) 248
Bbv12I GWGCWC 4 cut(s) 66, 78, 303, 587
BbvCI CCTCAGC 4 cut(s) 1176, 1188, 1194, 1200
BbvI GCAGC 7 cut(s) 121, 124, 874, 953, 1051, 1066, 1260
BccI CCATC 3 cut(s) 431, 823, 1111
BceAI ACGGC 3 cut(s) 355, 427, 628
BcgI CGANNNNNNTGC 3 cut(s) 31, 856, 890
BciT130I CCWGG 1 cut(s) 955
BcnI CCSGG 2 cut(s) 36, 478
BcoDI GTCTC 3 cut(s) 24, 577, 1221
BfoI RGCGCY 2 cut(s) 477, 1132
BfrI CTTAAG 1 cut(s) 1058
BmcAI AGTACT 1 cut(s) 838
Bme1390I CCNGG 3 cut(s) 36, 478, 955
Bme18I GGWCC 2 cut(s) 288, 537
BmeT110I CYCGRG 1 cut(s) 1206
BmiI GGNNCC 9 cut(s) 47, 217, 311, 475, 538, 539, 619, 924, 1263
BmrFI CCNGG 3 cut(s) 36, 478, 955
BmrI ACTGGG 2 cut(s) 535, 849
BmsI GCATC 1 cut(s) 668
BmuI ACTGGG 2 cut(s) 535, 849
Bpu10I CCTNAGC 4 cut(s) 1176, 1188, 1194, 1200
Bpu14I TTCGAA 2 cut(s) 374, 767
BpuEI CTTGAG 1 cut(s) 1064
BpuMI CCSGG 2 cut(s) 36, 478
BsaHI GRCGYC 2 cut(s) 54, 474
BsaI GGTCTC 1 cut(s) 577
BsaJI CCNNGG 9 cut(s) 23, 234, 294, 848, 866, 947, 953, 954, 1146
BsaWI WCCGGW 3 cut(s) 312, 913, 1270
BsaXI ACNNNNNCTCC 2 cut(s) 602, 632
Bsc4I CCNNNNNNNGG 7 cut(s) 42, 213, 468, 547, 627, 973, 1147
Bse1I ACTGG 2 cut(s) 541, 844
Bse3DI GCAATG 2 cut(s) 872, 907
BseBI CCWGG 1 cut(s) 955
BseDI CCNNGG 9 cut(s) 23, 234, 294, 848, 866, 947, 953, 954, 1146
BseGI GGATG 5 cut(s) 460, 559, 834, 937, 1122
BseLI CCNNNNNNNGG 7 cut(s) 42, 213, 468, 547, 627, 973, 1147
BseMI GCAATG 2 cut(s) 872, 907
BseMII CTCAG 6 cut(s) 1167, 1179, 1185, 1191, 1232, 1248
BseNI ACTGG 2 cut(s) 541, 844
BseRI GAGGAG 9 cut(s) 311, 406, 417, 441, 537, 722, 827, 1007, 1120
BseSI GKGCMC 2 cut(s) 49, 219
BseXI GCAGC 7 cut(s) 121, 124, 874, 953, 1051, 1066, 1260
BseYI CCCAGC 2 cut(s) 567, 690
Bsh1236I CGCG 6 cut(s) 41, 152, 280, 282, 360, 448
Bsh1285I CGRYCG 1 cut(s) 349
BshNI GGYRCC 2 cut(s) 473, 922
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 4 cut(s) 66, 78, 303, 587
BsiHKCI CYCGRG 1 cut(s) 1206
BsiSI CCGG 6 cut(s) 36, 313, 477, 621, 914, 1271
BslFI GGGAC 1 cut(s) 523
BslI CCNNNNNNNGG 7 cut(s) 42, 213, 468, 547, 627, 973, 1147
BsmAI GTCTC 3 cut(s) 24, 577, 1221
BsmBI CGTCTC 2 cut(s) 24, 1221
BsmFI GGGAC 1 cut(s) 523
Bso31I GGTCTC 1 cut(s) 577
BsoBI CYCGRG 1 cut(s) 1206
Bsp119I TTCGAA 2 cut(s) 374, 767
Bsp120I GGGCCC 2 cut(s) 45, 215
Bsp1286I GDGCHC 7 cut(s) 49, 66, 78, 219, 234, 303, 587
Bsp143I GATC 3 cut(s) 99, 253, 346
BspCNI CTCAG 6 cut(s) 1168, 1180, 1186, 1192, 1231, 1249
BspFNI CGCG 6 cut(s) 41, 152, 280, 282, 360, 448
BspLI GGNNCC 9 cut(s) 47, 217, 311, 475, 538, 539, 619, 924, 1263
BspPI GGATC 1 cut(s) 261
BspQI GCTCTTC 1 cut(s) 54
BspT104I TTCGAA 2 cut(s) 374, 767
BspT107I GGYRCC 2 cut(s) 473, 922
BspTI CTTAAG 1 cut(s) 1058
BspTNI GGTCTC 1 cut(s) 577
BsrBI CCGCTC 2 cut(s) 286, 637
BsrDI GCAATG 2 cut(s) 872, 907
BsrI ACTGG 2 cut(s) 541, 844
BssECI CCNNGG 9 cut(s) 23, 234, 294, 848, 866, 947, 953, 954, 1146
BssMI GATC 3 cut(s) 99, 253, 346
BssNI GRCGYC 2 cut(s) 54, 474
BssSI CACGAG 1 cut(s) 248
BssT1I CCWWGG 2 cut(s) 23, 947
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 1 cut(s) 955
Bst4CI ACNGT 3 cut(s) 13, 773, 1147
Bst6I CTCTTC 5 cut(s) 54, 506, 695, 800, 1097
BstACI GRCGYC 2 cut(s) 54, 474
BstAFI CTTAAG 1 cut(s) 1058
BstBI TTCGAA 2 cut(s) 374, 767
BstC8I GCNNGC 5 cut(s) 215, 225, 278, 284, 450
BstDEI CTNAG 6 cut(s) 1176, 1188, 1194, 1200, 1218, 1257
BstDSI CCRYGG 2 cut(s) 234, 1146
BstEII GGTNACC 1 cut(s) 1136
BstF5I GGATG 5 cut(s) 460, 559, 834, 937, 1122
BstFNI CGCG 6 cut(s) 41, 152, 280, 282, 360, 448
BstH2I RGCGCY 2 cut(s) 477, 1132
BstHHI GCGC 4 cut(s) 41, 282, 476, 1131
BstKTI GATC 3 cut(s) 102, 256, 349
BstMAI GTCTC 3 cut(s) 24, 577, 1221
BstMBI GATC 3 cut(s) 99, 253, 346
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 9 cut(s) 53, 149, 229, 342, 458, 621, 624, 859, 1079
BstNI CCWGG 1 cut(s) 955
BstPI GGTNACC 1 cut(s) 1136
BstSCI CCNGG 3 cut(s) 34, 476, 953
BstSLI GKGCMC 2 cut(s) 49, 219
BstUI CGCG 6 cut(s) 41, 152, 280, 282, 360, 448
BstV1I GCAGC 7 cut(s) 121, 124, 874, 953, 1051, 1066, 1260
BtgI CCRYGG 2 cut(s) 234, 1146
BtsCI GGATG 5 cut(s) 460, 559, 834, 937, 1122
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 5 cut(s) 215, 225, 278, 284, 450
CfoI GCGC 4 cut(s) 41, 282, 476, 1131
CpoI CGGWCCG 1 cut(s) 288
CseI GACGC 1 cut(s) 62
Csp6I GTAC 1 cut(s) 837
CspI CGGWCCG 1 cut(s) 288
CviQI GTAC 1 cut(s) 837
DdeI CTNAG 6 cut(s) 1176, 1188, 1194, 1200, 1218, 1257
DinI GGCGCC 1 cut(s) 475
DpnI GATC 3 cut(s) 101, 255, 348
DpnII GATC 3 cut(s) 99, 253, 346
DrdI GACNNNNNNGTC 1 cut(s) 569
DseDI GACNNNNNNGTC 1 cut(s) 569
Eam1104I CTCTTC 5 cut(s) 54, 506, 695, 800, 1097
EarI CTCTTC 5 cut(s) 54, 506, 695, 800, 1097
EciI GGCGGA 4 cut(s) 130, 467, 566, 1196
Ecl136II GAGCTC 3 cut(s) 76, 301, 585
Eco130I CCWWGG 2 cut(s) 23, 947
Eco24I GRGCYC 6 cut(s) 49, 78, 219, 234, 303, 587
Eco31I GGTCTC 1 cut(s) 577
Eco47I GGWCC 2 cut(s) 288, 537
Eco53kI GAGCTC 3 cut(s) 76, 301, 585
Eco88I CYCGRG 1 cut(s) 1206
Eco91I GGTNACC 1 cut(s) 1136
EcoICRI GAGCTC 3 cut(s) 76, 301, 585
EcoO109I RGGNCCY 2 cut(s) 46, 537
EcoO65I GGTNACC 1 cut(s) 1136
EcoRII CCWGG 1 cut(s) 953
EcoT14I CCWWGG 2 cut(s) 23, 947
EcoT38I GRGCYC 6 cut(s) 49, 78, 219, 234, 303, 587
EgeI GGCGCC 1 cut(s) 475
EheI GGCGCC 1 cut(s) 475
ErhI CCWWGG 2 cut(s) 23, 947
Esp3I CGTCTC 2 cut(s) 24, 1221
FaqI GGGAC 1 cut(s) 523
FauI CCCGC 8 cut(s) 206, 232, 285, 317, 441, 487, 620, 630
FokI GGATG 5 cut(s) 467, 566, 841, 944, 1129
FriOI GRGCYC 6 cut(s) 49, 78, 219, 234, 303, 587
GlaI GCGC 4 cut(s) 40, 281, 475, 1130
GsaI CCCAGC 2 cut(s) 571, 694
HaeII RGCGCY 2 cut(s) 477, 1132
HapII CCGG 6 cut(s) 36, 313, 477, 621, 914, 1271
HgaI GACGC 1 cut(s) 62
HhaI GCGC 4 cut(s) 41, 282, 476, 1131
Hin1I GRCGYC 2 cut(s) 54, 474
Hin6I GCGC 4 cut(s) 39, 280, 474, 1129
HinP1I GCGC 4 cut(s) 39, 280, 474, 1129
HinfI GANTC 7 cut(s) 365, 385, 557, 680, 785, 1046, 1091
HpaII CCGG 6 cut(s) 36, 313, 477, 621, 914, 1271
HphI GGTGA 2 cut(s) 364, 1148
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 7 cut(s) 390, 562, 598, 685, 790, 1221, 1258
Hpy188III TCNNGA 4 cut(s) 268, 331, 574, 1114
Hpy8I GTNNAC 1 cut(s) 355
Hpy99I CGWCG 4 cut(s) 56, 448, 467, 598
HpyCH4III ACNGT 3 cut(s) 13, 773, 1147
HpyCH4V TGCA 2 cut(s) 659, 862
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 149, 229, 342, 458, 621, 624, 859, 1079
HpyF3I CTNAG 6 cut(s) 1176, 1188, 1194, 1200, 1218, 1257
Hsp92I GRCGYC 2 cut(s) 54, 474
HspAI GCGC 4 cut(s) 39, 280, 474, 1129
KasI GGCGCC 1 cut(s) 473
KflI GGGWCCC 1 cut(s) 537
Kzo9I GATC 3 cut(s) 99, 253, 346
LguI GCTCTTC 1 cut(s) 54
LmnI GCTCC 3 cut(s) 298, 623, 1151
Lsp1109I GCAGC 7 cut(s) 121, 124, 874, 953, 1051, 1066, 1260
LweI GCATC 1 cut(s) 668
MaeIII GTNAC 2 cut(s) 880, 1136
MalI GATC 3 cut(s) 101, 255, 348
MbiI CCGCTC 2 cut(s) 286, 637
MboI GATC 3 cut(s) 99, 253, 346
MhlI GDGCHC 7 cut(s) 49, 66, 78, 219, 234, 303, 587
MluCI AATT 3 cut(s) 763, 856, 1252
Mly113I GGCGCC 1 cut(s) 474
MlyI GAGTC 3 cut(s) 394, 1055, 1100
MmeI TCCRAC 1 cut(s) 576
MseI TTAA 1 cut(s) 1059
MspA1I CMGCKG 1 cut(s) 567
MspCI CTTAAG 1 cut(s) 1058
MspI CCGG 6 cut(s) 36, 313, 477, 621, 914, 1271
MspR9I CCNGG 3 cut(s) 36, 478, 955
MvaI CCWGG 1 cut(s) 955
MvnI CGCG 6 cut(s) 41, 152, 280, 282, 360, 448
MwoI GCNNNNNNNGC 9 cut(s) 53, 149, 229, 342, 458, 621, 624, 859, 1079
NarI GGCGCC 1 cut(s) 474
NciI CCSGG 2 cut(s) 36, 478
NdeII GATC 3 cut(s) 99, 253, 346
NlaIV GGNNCC 9 cut(s) 47, 217, 311, 475, 538, 539, 619, 924, 1263
NmeAIII GCCGAG 6 cut(s) 97, 220, 253, 319, 873, 891
NmuCI GTSAC 1 cut(s) 1136
NspV TTCGAA 2 cut(s) 374, 767
PaeR7I CTCGAG 1 cut(s) 1206
PasI CCCWGGG 1 cut(s) 954
PciSI GCTCTTC 1 cut(s) 54
PfeI GAWTC 4 cut(s) 365, 557, 680, 785
Ple19I CGATCG 1 cut(s) 349
PleI GAGTC 3 cut(s) 393, 1054, 1099
PluTI GGCGCC 1 cut(s) 477
PpsI GAGTC 3 cut(s) 393, 1054, 1099
PpuMI RGGWCCY 1 cut(s) 537
Psp124BI GAGCTC 3 cut(s) 78, 303, 587
Psp5II RGGWCCY 1 cut(s) 537
Psp6I CCWGG 1 cut(s) 953
PspEI GGTNACC 1 cut(s) 1136
PspFI CCCAGC 2 cut(s) 567, 690
PspGI CCWGG 1 cut(s) 953
PspN4I GGNNCC 9 cut(s) 47, 217, 311, 475, 538, 539, 619, 924, 1263
PspOMI GGGCCC 2 cut(s) 45, 215
PspPPI RGGWCCY 1 cut(s) 537
PspXI VCTCGAGB 1 cut(s) 1206
PvuI CGATCG 1 cut(s) 349
PvuII CAGCTG 1 cut(s) 567
RsaI GTAC 1 cut(s) 838
RsaNI GTAC 1 cut(s) 837
Rsr2I CGGWCCG 1 cut(s) 288
RsrII CGGWCCG 1 cut(s) 288
SacI GAGCTC 3 cut(s) 78, 303, 587
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 1 cut(s) 1059
Sau3AI GATC 3 cut(s) 99, 253, 346
ScaI AGTACT 1 cut(s) 838
SchI GAGTC 3 cut(s) 394, 1055, 1100
ScrFI CCNGG 3 cut(s) 36, 478, 955
SduI GDGCHC 7 cut(s) 49, 66, 78, 219, 234, 303, 587
SfaNI GCATC 1 cut(s) 668
SfoI GGCGCC 1 cut(s) 475
Sfr274I CTCGAG 1 cut(s) 1206
SfuI TTCGAA 2 cut(s) 374, 767
SinI GGWCC 2 cut(s) 288, 537
SlaI CTCGAG 1 cut(s) 1206
SmlI CTYRAG 3 cut(s) 1043, 1058, 1206
SmoI CTYRAG 3 cut(s) 1043, 1058, 1206
Sse9I AATT 3 cut(s) 763, 856, 1252
SspDI GGCGCC 1 cut(s) 473
SstI GAGCTC 3 cut(s) 78, 303, 587
StyD4I CCNGG 3 cut(s) 34, 476, 953
StyI CCWWGG 2 cut(s) 23, 947
TaaI ACNGT 3 cut(s) 13, 773, 1147
TasI AATT 3 cut(s) 763, 856, 1252
TatI WGTACW 1 cut(s) 836
TfiI GAWTC 4 cut(s) 365, 557, 680, 785
Tru1I TTAA 1 cut(s) 1059
Tru9I TTAA 1 cut(s) 1059
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 1136
TseI GCWGC 7 cut(s) 109, 112, 862, 941, 1039, 1079, 1248
Tsp45I GTSAC 1 cut(s) 1136
TspDTI ATGAA 2 cut(s) 570, 918
TspGWI ACGGA 1 cut(s) 322
TspRI CASTG 1 cut(s) 18
Vha464I CTTAAG 1 cut(s) 1058
VpaK11BI GGWCC 2 cut(s) 288, 537
XhoI CTCGAG 1 cut(s) 1206
ZrmI AGTACT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.