Rh6BG021500

Plant specific eukaryotic initiation factor 4B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009133
Physical Location & Seq
Reverse (-)
3189656 .. 3191827
2172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG021500.1

Sequence Viewer

Length: 1203 bp
ATGGCGGCAACAGTGTCGTCTCCTTGGGCCAAGCCTGGCGCGTGGGCCCTCGACGCCGAAGAGCACGAAGCCGAGCTCGAACAACAACAACAACAGCAGGCCAAGATCGAAACGCAGCAGCCCCTCGCTGACTTCCCCTCTCTCTCCGCCGCCGCCGCGAAGCCGAAGAAGAAGAACAAGGGCCAGAAGGTCAGCCTCGCCGAGTTCTCCACCTTCGGCGGGCCCAAACCCGCCGAGCCCGTGGGCCTGACCCACGAGGATCGGTTGGTTCTCCCGACCGGCCCGCGCGAGCGGACCGCCGAGGAGCTCGAACGGAACCGGATTGTCGGCGGGTTCAAGAGCTACGGCGGCGATCGGGGGAACCGCGAGGATACGAGTTCGAAGTGGGGGAGTCAGAGGAGGGAAGGAGGAGGAGGGTTTGGGGGGAAAGAAGGAGGAGAGAGAGATGGGCCGTCGCGGGCGGATGAGGCCGACGACTGGGGCGCCGGGAAGAAATCGGCGGCGGGGAATGGGTTTGAGAGGAGGGAGAAAGGAGGGTTCTTTGGGTCCCAGTCGAAGGCGGATGAATCTGACAGCTGGGTCTCGAACAAGAGCTCGTTTTCGTCGGAGGGAAGGAGATACGGCGGCTCCGGCGGTGGGTTTGAGCGGGAGAGGAAGGTAGGGTTAGGGTTCAATTCGAACGGTGGCGGTGCGGATTCGGAGAGTTGGGGAAGGAAGAGGGAGGAGAGTAATGGTGGGATGGAGAGTACTGGGAGGCCGAGGCTCAATTTGCAGCCGAGGACATTGCCTGTTACTCTGCCTGTTGTGAGCAATGAAACCTCTCCGGTGGCGGTGCCTGTGGATGTGGCGGCAGCACCAAGGCCCAGGGGTTCGAACCCGTTTGGGGCGGCGAGGCCCAGAGAGGAGGTTTTGGCAGAGAAGGGGCAGGATTGGAAGAAGATTGACGAGCAGCTTGAGTCTGTGAAACTTAAGGAGAAGGAGGCTGTGGCTGCCGAAGGGGAGTCTTTTGGGAAGAGGAGTTTCGGGATGGGAAATGGGCGCTCTGGTGACCGCCCCGTGGGAGCTTGGAGGAAACCTGTTGTGGCTGAGGCGGAGGCTGAGGCTGAGGCTGAGGCTCGTCCTCAGAGTGCTGGAAACGATGAGAGTAGGAGCAGCAATTCTGAGGAACCAGAACCGGAGAATGAGAATGTCAATGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046983 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-4B

Protein Analysis

400

Amino Acids

42.78

Weight (kDa)

5.35

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-4B PF06273 6 - 323 9.5e-51 Plant specific eukaryotic initiation factor 4B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 578
AccB1I GGYRCC 2 cut(s) 482, 832
AccBSI CCGCTC 2 cut(s) 292, 646
AccII CGCG 6 cut(s) 41, 158, 286, 288, 366, 457
AclWI GGATC 1 cut(s) 267
AcyI GRCGYC 2 cut(s) 54, 483
AfaI GTAC 1 cut(s) 748
AfiI CCNNNNNNNGG 6 cut(s) 219, 477, 556, 636, 883, 1057
AflII CTTAAG 1 cut(s) 968
AgsI TTSAA 2 cut(s) 337, 673
AjnI CCWGG 2 cut(s) 34, 863
AluBI AGCT 7 cut(s) 76, 307, 342, 576, 594, 952, 1064
AluI AGCT 7 cut(s) 76, 307, 342, 576, 594, 952, 1064
Alw21I GWGCWC 4 cut(s) 66, 78, 309, 596
Alw26I GTCTC 2 cut(s) 24, 586
AlwI GGATC 1 cut(s) 267
ApaI GGGCCC 2 cut(s) 49, 225
ApeKI GCWGC 7 cut(s) 115, 118, 772, 851, 949, 989, 1152
AspLEI GCGC 4 cut(s) 41, 288, 485, 1041
AsuC2I CCSGG 1 cut(s) 487
AsuHPI GGTGA 1 cut(s) 1058
AsuII TTCGAA 3 cut(s) 380, 677, 872
AvaII GGWCC 2 cut(s) 294, 546
BaeGI GKGCMC 2 cut(s) 49, 225
BanI GGYRCC 2 cut(s) 482, 832
BanII GRGCYC 6 cut(s) 49, 78, 225, 240, 309, 596
BauI CACGAG 1 cut(s) 254
Bbv12I GWGCWC 4 cut(s) 66, 78, 309, 596
BbvCI CCTCAGC 4 cut(s) 1086, 1098, 1104, 1110
BbvI GCAGC 7 cut(s) 127, 130, 784, 863, 961, 976, 1164
BccI CCATC 3 cut(s) 440, 733, 1021
BceAI ACGGC 3 cut(s) 361, 436, 637
BcgI CGANNNNNNTGC 3 cut(s) 31, 766, 800
BciT130I CCWGG 2 cut(s) 36, 865
BciVI GTATCC 1 cut(s) 364
BcnI CCSGG 1 cut(s) 487
BcoDI GTCTC 2 cut(s) 24, 586
BfoI RGCGCY 2 cut(s) 486, 1042
BfrI CTTAAG 1 cut(s) 968
BfuI GTATCC 1 cut(s) 364
BmcAI AGTACT 1 cut(s) 748
Bme1390I CCNGG 3 cut(s) 36, 487, 865
Bme18I GGWCC 2 cut(s) 294, 546
BmrFI CCNGG 3 cut(s) 36, 487, 865
BmrI ACTGGG 3 cut(s) 487, 544, 759
BmuI ACTGGG 3 cut(s) 487, 544, 759
Bpu10I CCTNAGC 4 cut(s) 1086, 1098, 1104, 1110
Bpu14I TTCGAA 3 cut(s) 380, 677, 872
BpuEI CTTGAG 1 cut(s) 974
BpuMI CCSGG 1 cut(s) 487
BsaHI GRCGYC 2 cut(s) 54, 483
BsaI GGTCTC 1 cut(s) 586
BsaJI CCNNGG 9 cut(s) 23, 240, 300, 758, 776, 857, 863, 864, 1056
BsaWI WCCGGW 3 cut(s) 318, 823, 1174
BsaXI ACNNNNNCTCC 2 cut(s) 611, 641
Bsc4I CCNNNNNNNGG 6 cut(s) 219, 477, 556, 636, 883, 1057
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 3 cut(s) 482, 550, 754
Bse3DI GCAATG 2 cut(s) 782, 817
BseBI CCWGG 2 cut(s) 36, 865
BseDI CCNNGG 9 cut(s) 23, 240, 300, 758, 776, 857, 863, 864, 1056
BseGI GGATG 5 cut(s) 469, 568, 744, 847, 1032
BseLI CCNNNNNNNGG 6 cut(s) 219, 477, 556, 636, 883, 1057
BseMI GCAATG 2 cut(s) 782, 817
BseMII CTCAG 6 cut(s) 1077, 1089, 1095, 1101, 1136, 1152
BseNI ACTGG 3 cut(s) 482, 550, 754
BseRI GAGGAG 9 cut(s) 317, 412, 423, 426, 450, 535, 737, 917, 1030
BseSI GKGCMC 2 cut(s) 49, 225
BseXI GCAGC 7 cut(s) 127, 130, 784, 863, 961, 976, 1164
BseYI CCCAGC 1 cut(s) 576
Bsh1236I CGCG 6 cut(s) 41, 158, 286, 288, 366, 457
Bsh1285I CGRYCG 2 cut(s) 279, 355
BshNI GGYRCC 2 cut(s) 482, 832
BsiEI CGRYCG 2 cut(s) 279, 355
BsiHKAI GWGCWC 4 cut(s) 66, 78, 309, 596
BsiSI CCGG 6 cut(s) 279, 319, 486, 630, 824, 1175
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 6 cut(s) 219, 477, 556, 636, 883, 1057
BsmAI GTCTC 2 cut(s) 24, 586
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 532
Bso31I GGTCTC 1 cut(s) 586
Bsp119I TTCGAA 3 cut(s) 380, 677, 872
Bsp120I GGGCCC 2 cut(s) 45, 221
Bsp1286I GDGCHC 7 cut(s) 49, 66, 78, 225, 240, 309, 596
Bsp143I GATC 3 cut(s) 105, 259, 352
BspCNI CTCAG 6 cut(s) 1078, 1090, 1096, 1102, 1135, 1153
BspFNI CGCG 6 cut(s) 41, 158, 286, 288, 366, 457
BspPI GGATC 1 cut(s) 267
BspQI GCTCTTC 1 cut(s) 54
BspT104I TTCGAA 3 cut(s) 380, 677, 872
BspT107I GGYRCC 2 cut(s) 482, 832
BspTI CTTAAG 1 cut(s) 968
BspTNI GGTCTC 1 cut(s) 586
BsrBI CCGCTC 2 cut(s) 292, 646
BsrDI GCAATG 2 cut(s) 782, 817
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 3 cut(s) 482, 550, 754
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 9 cut(s) 23, 240, 300, 758, 776, 857, 863, 864, 1056
BssMI GATC 3 cut(s) 105, 259, 352
BssNI GRCGYC 2 cut(s) 54, 483
BssSI CACGAG 1 cut(s) 254
BssT1I CCWWGG 2 cut(s) 23, 857
Bst2BI CACGAG 1 cut(s) 254
Bst2UI CCWGG 2 cut(s) 36, 865
Bst4CI ACNGT 2 cut(s) 13, 683
Bst6I CTCTTC 3 cut(s) 54, 710, 1007
BstACI GRCGYC 2 cut(s) 54, 483
BstAFI CTTAAG 1 cut(s) 968
BstBI TTCGAA 3 cut(s) 380, 677, 872
BstC8I GCNNGC 5 cut(s) 99, 221, 284, 290, 459
BstDEI CTNAG 6 cut(s) 1086, 1098, 1104, 1110, 1122, 1161
BstDSI CCRYGG 2 cut(s) 240, 1056
BstEII GGTNACC 1 cut(s) 1046
BstF5I GGATG 5 cut(s) 469, 568, 744, 847, 1032
BstFNI CGCG 6 cut(s) 41, 158, 286, 288, 366, 457
BstH2I RGCGCY 2 cut(s) 486, 1042
BstHHI GCGC 4 cut(s) 41, 288, 485, 1041
BstKTI GATC 3 cut(s) 108, 262, 355
BstMAI GTCTC 2 cut(s) 24, 586
BstMBI GATC 3 cut(s) 105, 259, 352
BstMCI CGRYCG 2 cut(s) 279, 355
BstMWI GCNNNNNNNGC 7 cut(s) 53, 155, 348, 467, 630, 769, 989
BstNI CCWGG 2 cut(s) 36, 865
BstPI GGTNACC 1 cut(s) 1046
BstSCI CCNGG 3 cut(s) 34, 485, 863
BstSLI GKGCMC 2 cut(s) 49, 225
BstUI CGCG 6 cut(s) 41, 158, 286, 288, 366, 457
BstV1I GCAGC 7 cut(s) 127, 130, 784, 863, 961, 976, 1164
BsuI GTATCC 1 cut(s) 364
BtgI CCRYGG 2 cut(s) 240, 1056
BtsCI GGATG 5 cut(s) 469, 568, 744, 847, 1032
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 5 cut(s) 99, 221, 284, 290, 459
CfoI GCGC 4 cut(s) 41, 288, 485, 1041
Cfr10I RCCGGY 1 cut(s) 278
CpoI CGGWCCG 1 cut(s) 294
CseI GACGC 1 cut(s) 62
Csp6I GTAC 1 cut(s) 747
CspI CGGWCCG 1 cut(s) 294
CviQI GTAC 1 cut(s) 747
DdeI CTNAG 6 cut(s) 1086, 1098, 1104, 1110, 1122, 1161
DinI GGCGCC 1 cut(s) 484
DpnI GATC 3 cut(s) 107, 261, 354
DpnII GATC 3 cut(s) 105, 259, 352
DrdI GACNNNNNNGTC 1 cut(s) 578
DseDI GACNNNNNNGTC 1 cut(s) 578
Eam1104I CTCTTC 3 cut(s) 54, 710, 1007
EarI CTCTTC 3 cut(s) 54, 710, 1007
EciI GGCGGA 4 cut(s) 136, 476, 575, 1106
Ecl136II GAGCTC 3 cut(s) 76, 307, 594
Eco130I CCWWGG 2 cut(s) 23, 857
Eco24I GRGCYC 6 cut(s) 49, 78, 225, 240, 309, 596
Eco31I GGTCTC 1 cut(s) 586
Eco47I GGWCC 2 cut(s) 294, 546
Eco53kI GAGCTC 3 cut(s) 76, 307, 594
Eco91I GGTNACC 1 cut(s) 1046
EcoICRI GAGCTC 3 cut(s) 76, 307, 594
EcoO109I RGGNCCY 2 cut(s) 46, 546
EcoO65I GGTNACC 1 cut(s) 1046
EcoRII CCWGG 2 cut(s) 34, 863
EcoT14I CCWWGG 2 cut(s) 23, 857
EcoT38I GRGCYC 6 cut(s) 49, 78, 225, 240, 309, 596
EgeI GGCGCC 1 cut(s) 484
EheI GGCGCC 1 cut(s) 484
ErhI CCWWGG 2 cut(s) 23, 857
Esp3I CGTCTC 1 cut(s) 24
FaqI GGGAC 1 cut(s) 532
FauI CCCGC 7 cut(s) 212, 238, 291, 323, 450, 496, 639
FokI GGATG 5 cut(s) 476, 575, 751, 854, 1039
FriOI GRGCYC 6 cut(s) 49, 78, 225, 240, 309, 596
GlaI GCGC 4 cut(s) 40, 287, 484, 1040
GsaI CCCAGC 1 cut(s) 580
HaeII RGCGCY 2 cut(s) 486, 1042
HapII CCGG 6 cut(s) 279, 319, 486, 630, 824, 1175
HgaI GACGC 1 cut(s) 62
HhaI GCGC 4 cut(s) 41, 288, 485, 1041
Hin1I GRCGYC 2 cut(s) 54, 483
Hin6I GCGC 4 cut(s) 39, 286, 483, 1039
HinP1I GCGC 4 cut(s) 39, 286, 483, 1039
HinfI GANTC 5 cut(s) 391, 566, 695, 956, 1001
HpaII CCGG 6 cut(s) 279, 319, 486, 630, 824, 1175
HphI GGTGA 1 cut(s) 1058
Hpy188I TCNGA 6 cut(s) 396, 571, 607, 700, 1125, 1162
Hpy188III TCNNGA 4 cut(s) 274, 337, 583, 1024
Hpy99I CGWCG 4 cut(s) 56, 457, 476, 607
HpyCH4III ACNGT 2 cut(s) 13, 683
HpyCH4V TGCA 1 cut(s) 772
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 155, 348, 467, 630, 769, 989
HpyF3I CTNAG 6 cut(s) 1086, 1098, 1104, 1110, 1122, 1161
Hsp92I GRCGYC 2 cut(s) 54, 483
HspAI GCGC 4 cut(s) 39, 286, 483, 1039
KasI GGCGCC 1 cut(s) 482
KflI GGGWCCC 1 cut(s) 546
Kzo9I GATC 3 cut(s) 105, 259, 352
LguI GCTCTTC 1 cut(s) 54
LmnI GCTCC 4 cut(s) 304, 632, 1061, 1149
Lsp1109I GCAGC 7 cut(s) 127, 130, 784, 863, 961, 976, 1164
MaeIII GTNAC 2 cut(s) 790, 1046
MalI GATC 3 cut(s) 107, 261, 354
MbiI CCGCTC 2 cut(s) 292, 646
MboI GATC 3 cut(s) 105, 259, 352
MboII GAAGA 9 cut(s) 71, 178, 181, 184, 502, 727, 946, 949, 1024
MhlI GDGCHC 7 cut(s) 49, 66, 78, 225, 240, 309, 596
MluCI AATT 3 cut(s) 673, 766, 1156
Mly113I GGCGCC 1 cut(s) 483
MlyI GAGTC 3 cut(s) 400, 965, 1010
MmeI TCCRAC 1 cut(s) 585
MseI TTAA 1 cut(s) 969
MspA1I CMGCKG 1 cut(s) 576
MspCI CTTAAG 1 cut(s) 968
MspI CCGG 6 cut(s) 279, 319, 486, 630, 824, 1175
MspR9I CCNGG 3 cut(s) 36, 487, 865
MvaI CCWGG 2 cut(s) 36, 865
MvnI CGCG 6 cut(s) 41, 158, 286, 288, 366, 457
MwoI GCNNNNNNNGC 7 cut(s) 53, 155, 348, 467, 630, 769, 989
NarI GGCGCC 1 cut(s) 483
NciI CCSGG 1 cut(s) 487
NdeII GATC 3 cut(s) 105, 259, 352
NmeAIII GCCGAG 6 cut(s) 97, 226, 259, 325, 783, 801
NmuCI GTSAC 1 cut(s) 1046
NspV TTCGAA 3 cut(s) 380, 677, 872
PasI CCCWGGG 1 cut(s) 864
PciSI GCTCTTC 1 cut(s) 54
PfeI GAWTC 2 cut(s) 566, 695
Ple19I CGATCG 1 cut(s) 355
PleI GAGTC 3 cut(s) 399, 964, 1009
PluTI GGCGCC 1 cut(s) 486
PpsI GAGTC 3 cut(s) 399, 964, 1009
PpuMI RGGWCCY 1 cut(s) 546
Psp124BI GAGCTC 3 cut(s) 78, 309, 596
Psp5II RGGWCCY 1 cut(s) 546
Psp6I CCWGG 2 cut(s) 34, 863
PspEI GGTNACC 1 cut(s) 1046
PspFI CCCAGC 1 cut(s) 576
PspGI CCWGG 2 cut(s) 34, 863
PspOMI GGGCCC 2 cut(s) 45, 221
PspPPI RGGWCCY 1 cut(s) 546
PvuI CGATCG 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 576
RsaI GTAC 1 cut(s) 748
RsaNI GTAC 1 cut(s) 747
Rsr2I CGGWCCG 1 cut(s) 294
RsrII CGGWCCG 1 cut(s) 294
SacI GAGCTC 3 cut(s) 78, 309, 596
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 1 cut(s) 969
Sau3AI GATC 3 cut(s) 105, 259, 352
ScaI AGTACT 1 cut(s) 748
SchI GAGTC 3 cut(s) 400, 965, 1010
ScrFI CCNGG 3 cut(s) 36, 487, 865
SduI GDGCHC 7 cut(s) 49, 66, 78, 225, 240, 309, 596
SfoI GGCGCC 1 cut(s) 484
SfuI TTCGAA 3 cut(s) 380, 677, 872
SinI GGWCC 2 cut(s) 294, 546
SmlI CTYRAG 2 cut(s) 953, 968
SmoI CTYRAG 2 cut(s) 953, 968
Sse9I AATT 3 cut(s) 673, 766, 1156
SspDI GGCGCC 1 cut(s) 482
SstI GAGCTC 3 cut(s) 78, 309, 596
StyD4I CCNGG 3 cut(s) 34, 485, 863
StyI CCWWGG 2 cut(s) 23, 857
TaaI ACNGT 2 cut(s) 13, 683
TaqI TCGA 9 cut(s) 51, 78, 108, 309, 380, 554, 584, 677, 872
TasI AATT 3 cut(s) 673, 766, 1156
TatI WGTACW 1 cut(s) 746
TauI GCSGC 9 cut(s) 8, 152, 155, 158, 351, 503, 627, 851, 890
TfiI GAWTC 2 cut(s) 566, 695
Tru1I TTAA 1 cut(s) 969
Tru9I TTAA 1 cut(s) 969
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 1046
TseI GCWGC 7 cut(s) 115, 118, 772, 851, 949, 989, 1152
Tsp45I GTSAC 1 cut(s) 1046
TspDTI ATGAA 2 cut(s) 579, 828
TspGWI ACGGA 1 cut(s) 328
TspRI CASTG 1 cut(s) 18
Vha464I CTTAAG 1 cut(s) 968
VpaK11BI GGWCC 2 cut(s) 294, 546
ZrmI AGTACT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.