pycom05g17310

Photosystem II reaction center PSB28 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010122
Physical Location & Seq
Reverse (-)
20494394 .. 20495534
1141 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 696 bp
ATGGCTATGCTTGTTACACGCCACTCTGTCCTTGTTACCCACCACTCTATCCCCAAACCACAACTCCTTAGTCCTTTCTCCTCTTTAACACTCACACACACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTGCTTGTGTAATGGCAACCCTGCAGTCGCTTGCATTCTCCTCTCCAGTCTCACAGTGTCTGCAGCAACCACGCCTCCATTCAGGCTTGTTATGTTGGGGTGTTCATAGAAGTGCAGAATCCACATTCAATGGGCAGTCTTTGCGCGTGTCTCGTCCCCAATGGGCGCCGTTAAGACGGTGTACTCAAGTGTCTAGGTCTATTACAATGATGGTCAAACCGAAAATTCAGTTCATCCAAGGAACCGATGAACAGACAATACCAGATGTGAGGCTAACCAAATCAAAAGACGGTACAAATGGCGTGGCTATTTTCAGGTTTGAGCAACCTTCTGTGTTTGACTCCTCTGGTGAGTTTGGTGACATCACTGGATTTTACATGATTGACGAAGAAGGGGTCCTTTCGTCAGTTGATGTTAACGCCAAATTTGTCAACGGAAAGCCTGCAGGAATTGAAGCAAAGTACATAATGCGGACTCCAAGAGATTGGGACAGATTCATGAGATTCATGGAACGCTACGCAAATGACAATGGCTTGAGTTTCATCAAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Psb28

Protein Analysis

232

Amino Acids

25.76

Weight (kDa)

9.35

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Psb28 PF03912 123 - 228 2.5e-42 Psb28 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 307
AccII CGCG 1 cut(s) 288
AciI CCGC 1 cut(s) 613
AcsI RAATTY 2 cut(s) 366, 566
AcyI GRCGYC 1 cut(s) 308
AfaI GTAC 3 cut(s) 325, 436, 605
AgsI TTSAA 2 cut(s) 271, 596
Alw26I GTCTC 2 cut(s) 196, 297
AlwNI CAGNNNCTG 1 cut(s) 202
ApeKI GCWGC 1 cut(s) 205
ApoI RAATTY 2 cut(s) 366, 566
ArsI GACNNNNNNTTYG 2 cut(s) 522, 554
AspLEI GCGC 2 cut(s) 288, 310
AspS9I GGNCC 1 cut(s) 538
AsuHPI GGTGA 2 cut(s) 503, 512
AvaII GGWCC 1 cut(s) 538
BanI GGYRCC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 217
BccI CCATC 1 cut(s) 346
BceAI ACGGC 1 cut(s) 295
BcoDI GTCTC 2 cut(s) 196, 297
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 3 cut(s) 164, 203, 585
BfoI RGCGCY 1 cut(s) 311
BisI GCNGC 1 cut(s) 206
BlsI GCNGC 1 cut(s) 207
Bme18I GGWCC 1 cut(s) 538
BmgT120I GGNCC 1 cut(s) 538
BmiI GGNNCC 3 cut(s) 309, 385, 539
BpmI CTGGAG 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 308
BsaJI CCNNGG 1 cut(s) 379
BsaXI ACNNNNNCTCC 4 cut(s) 48, 78, 201, 231
Bse1I ACTGG 2 cut(s) 188, 514
BseDI CCNNGG 1 cut(s) 379
BseGI GGATG 1 cut(s) 375
BseNI ACTGG 2 cut(s) 188, 514
BseRI GAGGAG 3 cut(s) 70, 172, 475
BseXI GCAGC 1 cut(s) 217
BsgI GTGCAG 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 288
BshNI GGYRCC 1 cut(s) 307
BslFI GGGAC 2 cut(s) 282, 644
BsmAI GTCTC 2 cut(s) 196, 297
BsmFI GGGAC 2 cut(s) 282, 644
BsmI GAATGC 1 cut(s) 176
BspACI CCGC 1 cut(s) 613
BspFNI CGCG 1 cut(s) 288
BspHI TCATGA 1 cut(s) 639
BspLI GGNNCC 3 cut(s) 309, 385, 539
BspMAI CTGCAG 3 cut(s) 168, 207, 589
BspT107I GGYRCC 1 cut(s) 307
BsrI ACTGG 2 cut(s) 188, 514
BssECI CCNNGG 1 cut(s) 379
BssNI GRCGYC 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 379
Bst4CI ACNGT 3 cut(s) 198, 321, 434
BstACI GRCGYC 1 cut(s) 308
BstAPI GCANNNNNTGC 1 cut(s) 283
BstC8I GCNNGC 2 cut(s) 174, 585
BstDEI CTNAG 1 cut(s) 68
BstF5I GGATG 1 cut(s) 375
BstFNI CGCG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 311
BstHHI GCGC 2 cut(s) 288, 310
BstMAI GTCTC 2 cut(s) 196, 297
BstMWI GCNNNNNNNGC 1 cut(s) 283
BstSFI CTRYAG 3 cut(s) 164, 203, 585
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 1 cut(s) 217
BstXI CCANNNNNNTGG 1 cut(s) 627
BtsCI GGATG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 203, 507
Cac8I GCNNGC 2 cut(s) 174, 585
CaiI CAGNNNCTG 1 cut(s) 202
CciI TCATGA 1 cut(s) 639
CfoI GCGC 2 cut(s) 288, 310
Cfr13I GGNCC 1 cut(s) 538
Csp6I GTAC 3 cut(s) 324, 435, 604
CspCI CAANNNNNGTGG 2 cut(s) 426, 461
CviAII CATG 4 cut(s) 520, 640, 649, 693
CviJI RGCY 6 cut(s) 5, 228, 415, 449, 583, 675
CviKI_1 RGCY 6 cut(s) 5, 228, 415, 449, 583, 675
CviQI GTAC 3 cut(s) 324, 435, 604
DdeI CTNAG 1 cut(s) 68
DinI GGCGCC 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 538
EcoO109I RGGNCCY 1 cut(s) 538
EcoT14I CCWWGG 1 cut(s) 379
EgeI GGCGCC 1 cut(s) 309
EheI GGCGCC 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 379
FaeI CATG 4 cut(s) 523, 643, 652, 696
FaiI YATR 8 cut(s) 8, 235, 249, 521, 608, 641, 650, 694
FalI AAGNNNNNCTT 2 cut(s) 525, 557
FaqI GGGAC 2 cut(s) 282, 644
FatI CATG 4 cut(s) 519, 639, 648, 692
Fnu4HI GCNGC 1 cut(s) 206
FokI GGATG 1 cut(s) 362
Fsp4HI GCNGC 1 cut(s) 206
FspBI CTAG 1 cut(s) 336
GlaI GCGC 2 cut(s) 287, 309
GluI GCNGC 1 cut(s) 206
GsuI CTGGAG 1 cut(s) 171
HaeII RGCGCY 1 cut(s) 311
HhaI GCGC 2 cut(s) 288, 310
Hin1I GRCGYC 1 cut(s) 308
Hin1II CATG 4 cut(s) 523, 643, 652, 696
Hin6I GCGC 2 cut(s) 286, 308
HinP1I GCGC 2 cut(s) 286, 308
HincII GTYRAC 2 cut(s) 559, 574
HindII GTYRAC 2 cut(s) 559, 574
HinfI GANTC 5 cut(s) 260, 482, 616, 636, 645
HpaI GTTAAC 1 cut(s) 559
HphI GGTGA 2 cut(s) 503, 512
Hpy166II GTNNAC 3 cut(s) 324, 559, 574
Hpy188III TCNNGA 1 cut(s) 640
Hpy8I GTNNAC 3 cut(s) 324, 559, 574
HpyAV CCTTC 2 cut(s) 480, 527
HpyCH4III ACNGT 3 cut(s) 198, 321, 434
HpyCH4V TGCA 5 cut(s) 166, 176, 205, 257, 587
HpyF10VI GCNNNNNNNGC 1 cut(s) 283
HpyF3I CTNAG 1 cut(s) 68
Hsp92I GRCGYC 1 cut(s) 308
Hsp92II CATG 4 cut(s) 523, 643, 652, 696
HspAI GCGC 2 cut(s) 286, 308
KasI GGCGCC 1 cut(s) 307
KspAI GTTAAC 1 cut(s) 559
LpnPI CCDG 9 cut(s) 176, 201, 210, 417, 442, 474, 495, 573, 597
Lsp1109I GCAGC 1 cut(s) 217
MaeI CTAG 1 cut(s) 336
MaeIII GTNAC 3 cut(s) 13, 34, 500
MboII GAAGA 1 cut(s) 542
MluCI AATT 3 cut(s) 366, 566, 591
Mly113I GGCGCC 1 cut(s) 308
MlyI GAGTC 2 cut(s) 476, 610
MnlI CCTC 5 cut(s) 91, 193, 227, 405, 496
MseI TTAA 3 cut(s) 86, 314, 558
MslI CAYNNNNRTG 1 cut(s) 252
Mva1269I GAATGC 1 cut(s) 176
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 283
NarI GGCGCC 1 cut(s) 308
NlaIII CATG 4 cut(s) 523, 643, 652, 696
NlaIV GGNNCC 3 cut(s) 309, 385, 539
NmuCI GTSAC 1 cut(s) 500
PagI TCATGA 1 cut(s) 639
PctI GAATGC 1 cut(s) 176
PfeI GAWTC 3 cut(s) 260, 636, 645
PkrI GCNGC 1 cut(s) 207
PleI GAGTC 2 cut(s) 476, 610
PluTI GGCGCC 1 cut(s) 311
PpsI GAGTC 2 cut(s) 476, 610
PpuMI RGGWCCY 1 cut(s) 538
Psp5II RGGWCCY 1 cut(s) 538
PspN4I GGNNCC 3 cut(s) 309, 385, 539
PspPI GGNCC 1 cut(s) 538
PspPPI RGGWCCY 1 cut(s) 538
PsrI GAACNNNNNNTAC 2 cut(s) 384, 416
PstI CTGCAG 3 cut(s) 168, 207, 589
PstNI CAGNNNCTG 1 cut(s) 202
RsaI GTAC 3 cut(s) 325, 436, 605
RsaNI GTAC 3 cut(s) 324, 435, 604
RseI CAYNNNNRTG 1 cut(s) 252
SaqAI TTAA 3 cut(s) 86, 314, 558
SatI GCNGC 1 cut(s) 206
Sau96I GGNCC 1 cut(s) 538
SbfI CCTGCAGG 1 cut(s) 589
SchI GAGTC 2 cut(s) 476, 610
SdaI CCTGCAGG 1 cut(s) 589
SetI ASST 3 cut(s) 341, 461, 472
SfcI CTRYAG 3 cut(s) 164, 203, 585
SfoI GGCGCC 1 cut(s) 309
SinI GGWCC 1 cut(s) 538
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 2 cut(s) 327, 676
SmoI CTYRAG 2 cut(s) 327, 676
Sse8387I CCTGCAGG 1 cut(s) 589
Sse9I AATT 3 cut(s) 366, 566, 591
SsiI CCGC 1 cut(s) 613
SspDI GGCGCC 1 cut(s) 307
SspMI CTAG 1 cut(s) 336
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 3 cut(s) 198, 321, 434
TasI AATT 3 cut(s) 366, 566, 591
TatI WGTACW 2 cut(s) 323, 603
TfiI GAWTC 3 cut(s) 260, 636, 645
Tru1I TTAA 3 cut(s) 86, 314, 558
Tru9I TTAA 3 cut(s) 86, 314, 558
TscAI CASTG 2 cut(s) 203, 514
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 6 cut(s) 236, 364, 405, 628, 637, 673
TspGWI ACGGA 1 cut(s) 591
TspRI CASTG 2 cut(s) 203, 514
VpaK11BI GGWCC 1 cut(s) 538
XapI RAATTY 2 cut(s) 366, 566
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.