pycom05g25910

CSC1-like protein ERD4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011925
Physical Location & Seq
Reverse (-)
27203034 .. 27206227
3194 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2175 bp
ATGGACCTCGCGTCGTTCTTGACGTCCTTGGGGACCTCCTTCCTCATATTTGTGGCTCTGATGCTTTTGTTCTCATGGCTTTCGAGAAAGCCTGGAAACACTGTGATTTACTATCCGAATCGGATCTTGAGAGGGTTGGATCCATGGGAGGGCGGGTCCATGACCCGGAACCCATTTGCTTGGATCAAGGAGGCTCTGTCTTCCACCGAGCAGGAAGTTATATCCATGTCAGGGGTCGACACTGCTGTGTACTTCGTGTTCTTGAGCACTGTGTTGGGTATATTGGTTTTGTCTAGCCTTGTTCTGCTACCGATTCTTCTGCCAGTTGCTGCCACCGAAGTCGGGCAGAAGTTAACCAACGCAACCATAAGCAATGGCACTTTCAGTGACCTTGACAAGTTATCCATGGGACATCTCAAGGAGAAGAGTCCTAGGTTGTGGGCATTTCTCTTGGGTGTCTACTGGGTTTCTTTCGTCACGTATTTCTTGCTGTGGAAGGCATATAAACATGCCTCCAACCTGAGAGCCACTGCTTTAATGTCTCCTCAAGCGAAGCCTGAACAATTTGCCATTCTTGTCAGAGACATACCTAACTCCCTCGAAGGTCAAATTAGGAAGGAACAGGTCGATTCTTATTTCAAGACCGTCTACCCTGATACATTTTATAGATCATTGGTTGTCACAGACAACAAAGAGGTGGACAAAATTTGGGAAGAGTTGGAAGGGTTTAAGAAGAAGCTTGTACGCGCTGAAGCAGTCTATGCATTATCGAAGAAAACTGGCAACTCAGAAGGAATAAGACCCACTAACAAAACCGGTTTCCTTGGTCTTTGTGGGGCAAAAGTAGACAGTATTGAGTACTACACGGAGAAGATTAATGAATTAACCCCAAAACTGGAGGCTGAACAGAAAGCCACTCTCAGAGAGAAGCAGCAAAATGCCGCTCTAGTTTTTTTCACCAGTAGGGTGACTGCAGCTGCTGCAGCTCAGACTCTACATGCCCAACTTGTTGACACATGGACAGTCACCGATGCTCCTGAGCCTCGTCAAGTACTTTGGCCTAGTCTCAAAATCAAGTTTTTTCAGAGACAGGTGCGGCAGAATGTTGTGTATATCATCGTGGCTCTGACTGTAGTGTTTTATATGATTCCAATTGCTGCCATCTCTGCATTCACAACTCTGGACAACCTGAAGAAACTTCTTCCATTTCTAAAGCCAATTTTGAAGCAAGATGCGATTAGGACAGTGCTGGAGGCTTATCTACCTCAAATTGCACTCATAGTCTTTCTGGCTATGTTGCCAAAGTTCCTGCTGTTTTTGTCCAAGGCTGAGGGAATTCCTTCCCAGAGCCATGCAATAAGGGCTGCTTCCGGGAAATACTTCTACTTCACTATCTTCAATGTTTTTCTTGGAGTTACGGTAGGTGGAACCTTATTCAGTACATTCAAGACCATCGAGAAGGATCCTAACTCTCTTGTTGACTTACTCGCAACTAGCCTCCCTGCCAATGCAACTTACTTCCTCACCTTTGTGGCCCTAAAGTTTTTCGTTGGATATGGTCTTGAACTGTCCCGACTAGTTCCTCTGATCATTTTCCATATAAAGAGGAAGTACCTCTGTAAGACCGAAGCTGAGGTGAAAGCAGCTTGGCTTCCCAGTGATCTTGGATACGGGACTAGAGTACCTAGTGATCTGCTCATCATTACGATTGTCCTCTGCTACTCTGTTATTGCTCCACTGATTCTTCCATTTGGTGTGCTGTACTTTGGCCTTGGATGGCTTATCCTTCGAAATCAGGCACTCAAAGTTTATATTCCAGCATACGAAAGCTATGGAAGGATGTGGCCGCACATGCAAATTCGAATCATTGCATCTCTGATATTGTACCAAGTTACCATGTTTGGTTACTTTGGGGTGAAGAGATTCTACTATGCACCATTCTTAATTCCACTTCCTATCATGTCCTTGCTTTTCAGTTTCATCTGCAGCAAGAAATTCTACCGGTTTTTCCAACACACCGCGCTTGAAGTAGCTGCACACGAACTCAAGGAAATTCCGAACATGGAGCAAGTTTACAGAGCTTTCATCCCACCAAGCCTGAGCTCCGAGAAGATAGACGATGATCAATTTGAAGACGCTCAATCTAGTGTTTCGAGAACAGCCTCGTTCGCCTAA

Protein Analysis

725

Amino Acids

81.75

Weight (kDa)

9.27

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 5 - 166 2.9e-33 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 188 - 354 6.9e-38 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 367 - 637 2.2e-80 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011925)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30360
fragaria_vesca FvH4_3g04980 FvH4_3g04980
malus_domestica MD05G1278500.v1.1 MD10G1257300.v1.1
prunus_persica Prupe.4G086300_v2.0.a1
pyrus_communis pycom05g25910 pycom10g21280 pycom10g21310 pycom10g21330
rosa_chinensis RchiOBHm_Chr5g0015001
rosa_laevigata RLG00000032159
rosa_multiflora Rmu_sc0004889.1_g000022
rosa_roxburghii Rroxscaffold_1G00061540
rosa_rugosa Rorug05G0022600 Rorug05G0022700
rosa_samantha Rh5AG117900 Rh5BG115100
rosa_wichuraiana Rw5G010160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 26
AccBSI CCGCTC 1 cut(s) 944
AccI GTMKAC 4 cut(s) 237, 459, 648, 846
AccII CGCG 3 cut(s) 11, 747, 2021
AciI CCGC 5 cut(s) 153, 942, 1096, 1847, 2019
AclWI GGATC 6 cut(s) 131, 134, 147, 191, 1457, 1470
AcoI YGGCCR 1 cut(s) 1844
AcsI RAATTY 5 cut(s) 705, 1335, 1857, 1994, 2052
AcuI CTGAAG 2 cut(s) 771, 1211
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 9 cut(s) 251, 744, 860, 1053, 1441, 1613, 1683, 1763, 1886
AfiI CCNNNNNNNGG 5 cut(s) 149, 165, 231, 342, 895
AgeI ACCGGT 2 cut(s) 815, 2001
AgsI TTSAA 7 cut(s) 640, 1225, 1399, 1447, 1565, 2027, 2132
AhdI GACNNNNNGTC 1 cut(s) 10
AhlI ACTAGT 1 cut(s) 1576
AjnI CCWGG 1 cut(s) 91
AleI CACNNNNGTG 2 cut(s) 245, 1529
AluBI AGCT 9 cut(s) 739, 977, 986, 1631, 1646, 1830, 2033, 2081, 2103
AluI AGCT 9 cut(s) 739, 977, 986, 1631, 1646, 1830, 2033, 2081, 2103
Alw21I GWGCWC 2 cut(s) 269, 2105
Alw26I GTCTC 4 cut(s) 546, 576, 1070, 1081
AlwI GGATC 6 cut(s) 131, 134, 147, 191, 1457, 1470
AlwNI CAGNNNCTG 2 cut(s) 329, 980
AoxI GGCC 4 cut(s) 1058, 1533, 1768, 1844
ApoI RAATTY 5 cut(s) 705, 1335, 1857, 1994, 2052
AseI ATTAAT 1 cut(s) 876
AsiGI ACCGGT 2 cut(s) 815, 2001
Asp700I GAANNNNTTC 3 cut(s) 1339, 1379, 1922
AspA2I CCTAGG 1 cut(s) 431
AspLEI GCGC 2 cut(s) 749, 2023
AspS9I GGNCC 4 cut(s) 4, 33, 156, 1534
AsuC2I CCSGG 2 cut(s) 166, 1372
AsuHPI GGTGA 6 cut(s) 949, 979, 1018, 1516, 1648, 1927
AsuII TTCGAA 2 cut(s) 1789, 1861
AvaII GGWCC 3 cut(s) 4, 33, 156
AvrII CCTAGG 1 cut(s) 431
BaeI ACNNNNGTAYC 2 cut(s) 1665, 1698
BamHI GGATCC 2 cut(s) 139, 1462
BanII GRGCYC 1 cut(s) 2105
BarI GAAGNNNNNNTAC 2 cut(s) 1910, 1942
BbsI GAAGAC 2 cut(s) 192, 2139
Bbv12I GWGCWC 2 cut(s) 269, 2105
BbvCI CCTCAGC 2 cut(s) 1329, 1632
BccI CCATC 3 cut(s) 1169, 1460, 1770
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciT130I CCWGG 1 cut(s) 93
BciVI GTATCC 1 cut(s) 1661
BclI TGATCA 2 cut(s) 1587, 2122
BcnI CCSGG 2 cut(s) 166, 1372
BcoDI GTCTC 4 cut(s) 546, 576, 1070, 1081
BcuI ACTAGT 1 cut(s) 1576
BfaI CTAG 9 cut(s) 294, 432, 947, 1062, 1494, 1577, 1677, 1686, 2145
BfmI CTRYAG 4 cut(s) 972, 981, 1131, 1984
BfuI GTATCC 1 cut(s) 1661
BlnI CCTAGG 1 cut(s) 431
BmcAI AGTACT 2 cut(s) 860, 1053
Bme1390I CCNGG 3 cut(s) 93, 166, 1372
Bme18I GGWCC 3 cut(s) 4, 33, 156
BmeRI GACNNNNNGTC 1 cut(s) 10
BmgT120I GGNCC 4 cut(s) 4, 33, 156, 1534
BmiI GGNNCC 6 cut(s) 34, 141, 157, 170, 1429, 1464
BmrFI CCNGG 3 cut(s) 93, 166, 1372
BmrI ACTGGG 2 cut(s) 472, 1650
BmsI GCATC 4 cut(s) 51, 1021, 1222, 1880
BmuI ACTGGG 2 cut(s) 472, 1650
BpiI GAAGAC 2 cut(s) 192, 2139
BpmI CTGGAG 2 cut(s) 917, 1271
Bpu10I CCTNAGC 4 cut(s) 1038, 1329, 1632, 2099
Bpu14I TTCGAA 2 cut(s) 1789, 1861
BpuEI CTTGAG 5 cut(s) 148, 283, 401, 531, 2030
BpuMI CCSGG 2 cut(s) 166, 1372
BsaAI YACGTR 1 cut(s) 480
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 7 cut(s) 27, 143, 405, 431, 823, 1323, 1771
BsaWI WCCGGW 2 cut(s) 815, 2001
BsaXI ACNNNNNCTCC 8 cut(s) 140, 170, 182, 212, 1018, 1048, 1404, 1434
Bsc4I CCNNNNNNNGG 5 cut(s) 149, 165, 231, 342, 895
Bse118I RCCGGY 2 cut(s) 815, 2001
Bse1I ACTGG 6 cut(s) 323, 467, 784, 900, 960, 1656
Bse3DI GCAATG 2 cut(s) 379, 1866
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 7 cut(s) 27, 143, 405, 431, 823, 1323, 1771
BseGI GGATG 3 cut(s) 1781, 1845, 2085
BseLI CCNNNNNNNGG 5 cut(s) 149, 165, 231, 342, 895
BseMI GCAATG 2 cut(s) 379, 1866
BseMII CTCAG 8 cut(s) 512, 801, 934, 1001, 1029, 1320, 1623, 2090
BseNI ACTGG 6 cut(s) 323, 467, 784, 900, 960, 1656
BseRI GAGGAG 1 cut(s) 534
BsgI GTGCAG 1 cut(s) 2019
Bsh1236I CGCG 3 cut(s) 11, 747, 2021
BshFI GGCC 4 cut(s) 1060, 1535, 1770, 1846
BshTI ACCGGT 2 cut(s) 815, 2001
BsiHKAI GWGCWC 2 cut(s) 269, 2105
BsiSI CCGG 4 cut(s) 166, 816, 1371, 2002
BslFI GGGAC 4 cut(s) 46, 423, 1555, 1687
BslI CCNNNNNNNGG 5 cut(s) 149, 165, 231, 342, 895
BsmAI GTCTC 4 cut(s) 546, 576, 1070, 1081
BsmFI GGGAC 4 cut(s) 46, 423, 1555, 1687
BsmI GAATGC 1 cut(s) 1169
BsnI GGCC 4 cut(s) 1060, 1535, 1770, 1846
Bsp119I TTCGAA 2 cut(s) 1789, 1861
Bsp1286I GDGCHC 2 cut(s) 269, 2105
Bsp143I GATC 9 cut(s) 123, 139, 183, 668, 1462, 1587, 1660, 1690, 2122
Bsp19I CCATGG 2 cut(s) 143, 405
BspACI CCGC 5 cut(s) 153, 942, 1096, 1847, 2019
BspANI GGCC 4 cut(s) 1060, 1535, 1770, 1846
BspCNI CTCAG 8 cut(s) 513, 800, 933, 1000, 1030, 1321, 1624, 2091
BspFNI CGCG 3 cut(s) 11, 747, 2021
BspLI GGNNCC 6 cut(s) 34, 141, 157, 170, 1429, 1464
BspMAI CTGCAG 3 cut(s) 976, 985, 1988
BspPI GGATC 6 cut(s) 131, 134, 147, 191, 1457, 1470
BspT104I TTCGAA 2 cut(s) 1789, 1861
BsrBI CCGCTC 1 cut(s) 944
BsrDI GCAATG 2 cut(s) 379, 1866
BsrFI RCCGGY 2 cut(s) 815, 2001
BsrI ACTGG 6 cut(s) 323, 467, 784, 900, 960, 1656
BssAI RCCGGY 2 cut(s) 815, 2001
BssECI CCNNGG 7 cut(s) 27, 143, 405, 431, 823, 1323, 1771
BssMI GATC 9 cut(s) 123, 139, 183, 668, 1462, 1587, 1660, 1690, 2122
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 7 cut(s) 27, 143, 405, 431, 823, 1323, 1771
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 9 cut(s) 103, 271, 646, 851, 1024, 1132, 1246, 1420, 1569
Bst6I CTCTTC 3 cut(s) 419, 708, 1913
BstACI GRCGYC 1 cut(s) 23
BstAPI GCANNNNNTGC 2 cut(s) 761, 980
BstBAI YACGTR 1 cut(s) 480
BstBI TTCGAA 2 cut(s) 1789, 1861
BstDEI CTNAG 8 cut(s) 521, 787, 920, 987, 1038, 1329, 1632, 2099
BstDSI CCRYGG 2 cut(s) 143, 405
BstF5I GGATG 3 cut(s) 1781, 1845, 2085
BstFNI CGCG 3 cut(s) 11, 747, 2021
BstHHI GCGC 2 cut(s) 749, 2023
BstKTI GATC 9 cut(s) 126, 142, 186, 671, 1465, 1590, 1663, 1693, 2125
BstMAI GTCTC 4 cut(s) 546, 576, 1070, 1081
BstMBI GATC 9 cut(s) 123, 139, 183, 668, 1462, 1587, 1660, 1690, 2122
BstMWI GCNNNNNNNGC 7 cut(s) 761, 980, 983, 1166, 1361, 1852, 2168
BstNI CCWGG 1 cut(s) 93
BstNSI RCATGY 3 cut(s) 512, 1001, 1855
BstSCI CCNGG 3 cut(s) 91, 164, 1370
BstSFI CTRYAG 4 cut(s) 972, 981, 1131, 1984
BstUI CGCG 3 cut(s) 11, 747, 2021
BstV2I GAAGAC 2 cut(s) 192, 2139
BstX2I RGATCY 3 cut(s) 123, 139, 1462
BstXI CCANNNNNNTGG 1 cut(s) 180
BstYI RGATCY 3 cut(s) 123, 139, 1462
BsuI GTATCC 1 cut(s) 1661
BsuRI GGCC 4 cut(s) 1060, 1535, 1770, 1846
BtgI CCRYGG 2 cut(s) 143, 405
BtsCI GGATG 3 cut(s) 1781, 1845, 2085
BtsI GCAGTG 2 cut(s) 240, 528
BtsIMutI CAGTG 8 cut(s) 99, 240, 267, 391, 528, 1251, 1663, 1736
CaiI CAGNNNCTG 2 cut(s) 329, 980
CfoI GCGC 2 cut(s) 749, 2023
Cfr10I RCCGGY 2 cut(s) 815, 2001
Cfr13I GGNCC 4 cut(s) 4, 33, 156, 1534
CseI GACGC 1 cut(s) 2144
Csp6I GTAC 9 cut(s) 250, 743, 859, 1052, 1440, 1612, 1682, 1762, 1885
CspAI ACCGGT 2 cut(s) 815, 2001
CviQI GTAC 9 cut(s) 250, 743, 859, 1052, 1440, 1612, 1682, 1762, 1885
DdeI CTNAG 8 cut(s) 521, 787, 920, 987, 1038, 1329, 1632, 2099
DpnI GATC 9 cut(s) 125, 141, 185, 670, 1464, 1589, 1662, 1692, 2124
DpnII GATC 9 cut(s) 123, 139, 183, 668, 1462, 1587, 1660, 1690, 2122
DriI GACNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 1844
Eam1104I CTCTTC 3 cut(s) 419, 708, 1913
Eam1105I GACNNNNNGTC 1 cut(s) 10
EarI CTCTTC 3 cut(s) 419, 708, 1913
Ecl136II GAGCTC 1 cut(s) 2103
Eco130I CCWWGG 7 cut(s) 27, 143, 405, 431, 823, 1323, 1771
Eco24I GRGCYC 1 cut(s) 2105
Eco47I GGWCC 3 cut(s) 4, 33, 156
Eco53kI GAGCTC 1 cut(s) 2103
Eco57I CTGAAG 2 cut(s) 771, 1211
EcoICRI GAGCTC 1 cut(s) 2103
EcoO109I RGGNCCY 1 cut(s) 33
EcoRI GAATTC 1 cut(s) 1335
EcoRII CCWGG 1 cut(s) 91
EcoT14I CCWWGG 7 cut(s) 27, 143, 405, 431, 823, 1323, 1771
EcoT22I ATGCAT 1 cut(s) 766
EcoT38I GRGCYC 1 cut(s) 2105
ErhI CCWWGG 7 cut(s) 27, 143, 405, 431, 823, 1323, 1771
FalI AAGNNNNNCTT 2 cut(s) 1351, 1383
FaqI GGGAC 4 cut(s) 46, 423, 1555, 1687
FauI CCCGC 1 cut(s) 146
FbaI TGATCA 2 cut(s) 1587, 2122
FblI GTMKAC 4 cut(s) 237, 459, 648, 846
FokI GGATG 3 cut(s) 1788, 1852, 2072
FriOI GRGCYC 1 cut(s) 2105
FspBI CTAG 9 cut(s) 294, 432, 947, 1062, 1494, 1577, 1677, 1686, 2145
GlaI GCGC 2 cut(s) 748, 2022
GsuI CTGGAG 2 cut(s) 917, 1271
HaeIII GGCC 4 cut(s) 1060, 1535, 1770, 1846
HapII CCGG 4 cut(s) 166, 816, 1371, 2002
HgaI GACGC 1 cut(s) 2144
HhaI GCGC 2 cut(s) 749, 2023
Hin1I GRCGYC 1 cut(s) 23
Hin6I GCGC 2 cut(s) 747, 2021
HinP1I GCGC 2 cut(s) 747, 2021
HincII GTYRAC 4 cut(s) 238, 354, 1012, 1480
HindII GTYRAC 4 cut(s) 238, 354, 1012, 1480
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 9 cut(s) 118, 313, 427, 629, 991, 1147, 1741, 1863, 1923
HpaI GTTAAC 1 cut(s) 354
HpaII CCGG 4 cut(s) 166, 816, 1371, 2002
HphI GGTGA 6 cut(s) 949, 979, 1018, 1516, 1648, 1927
Hpy99I CGWCG 1 cut(s) 16
HpyCH4III ACNGT 9 cut(s) 103, 271, 646, 851, 1024, 1132, 1246, 1420, 1569
HpyCH4IV ACGT 2 cut(s) 23, 479
HpyF10VI GCNNNNNNNGC 7 cut(s) 761, 980, 983, 1166, 1361, 1852, 2168
HpyF3I CTNAG 8 cut(s) 521, 787, 920, 987, 1038, 1329, 1632, 2099
HpySE526I ACGT 2 cut(s) 23, 479
Hsp92I GRCGYC 1 cut(s) 23
HspAI GCGC 2 cut(s) 747, 2021
Ksp22I TGATCA 2 cut(s) 1587, 2122
KspAI GTTAAC 1 cut(s) 354
Kzo9I GATC 9 cut(s) 123, 139, 183, 668, 1462, 1587, 1660, 1690, 2122
LmnI GCTCC 4 cut(s) 1039, 1738, 2065, 2108
LweI GCATC 4 cut(s) 51, 1021, 1222, 1880
MaeI CTAG 9 cut(s) 294, 432, 947, 1062, 1494, 1577, 1677, 1686, 2145
MaeII ACGT 2 cut(s) 23, 479
MaeIII GTNAC 8 cut(s) 386, 475, 679, 967, 1024, 1414, 1891, 1904
MalI GATC 9 cut(s) 125, 141, 185, 670, 1464, 1589, 1662, 1692, 2124
MbiI CCGCTC 1 cut(s) 944
MboI GATC 9 cut(s) 123, 139, 183, 668, 1462, 1587, 1660, 1690, 2122
MfeI CAATTG 1 cut(s) 1152
MflI RGATCY 3 cut(s) 123, 139, 1462
MhlI GDGCHC 2 cut(s) 269, 2105
MlyI GAGTC 2 cut(s) 436, 985
MmeI TCCRAC 5 cut(s) 117, 540, 699, 1531, 2035
Mph1103I ATGCAT 1 cut(s) 766
MroXI GAANNNNTTC 3 cut(s) 1339, 1379, 1922
MseI TTAA 6 cut(s) 353, 536, 729, 876, 884, 1943
MslI CAYNNNNRTG 3 cut(s) 50, 245, 1529
MspA1I CMGCKG 1 cut(s) 977
MspI CCGG 4 cut(s) 166, 816, 1371, 2002
MspR9I CCNGG 3 cut(s) 93, 166, 1372
MunI CAATTG 1 cut(s) 1152
Mva1269I GAATGC 1 cut(s) 1169
MvaI CCWGG 1 cut(s) 93
MvnI CGCG 3 cut(s) 11, 747, 2021
MwoI GCNNNNNNNGC 7 cut(s) 761, 980, 983, 1166, 1361, 1852, 2168
NciI CCSGG 2 cut(s) 166, 1372
NcoI CCATGG 2 cut(s) 143, 405
NdeII GATC 9 cut(s) 123, 139, 183, 668, 1462, 1587, 1660, 1690, 2122
NlaIV GGNNCC 6 cut(s) 34, 141, 157, 170, 1429, 1464
NmuCI GTSAC 5 cut(s) 386, 475, 679, 967, 1024
NsiI ATGCAT 1 cut(s) 766
NspI RCATGY 3 cut(s) 512, 1001, 1855
NspV TTCGAA 2 cut(s) 1789, 1861
OliI CACNNNNGTG 2 cut(s) 245, 1529
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 1 cut(s) 1169
PdmI GAANNNNTTC 3 cut(s) 1339, 1379, 1922
PfeI GAWTC 7 cut(s) 118, 313, 629, 1147, 1741, 1863, 1923
PfoI TCCNGGA 1 cut(s) 1370
PinAI ACCGGT 2 cut(s) 815, 2001
PleI GAGTC 2 cut(s) 435, 985
PpsI GAGTC 2 cut(s) 435, 985
Ppu21I YACGTR 1 cut(s) 480
PpuMI RGGWCCY 1 cut(s) 33
PshBI ATTAAT 1 cut(s) 876
Psp124BI GAGCTC 1 cut(s) 2105
Psp5II RGGWCCY 1 cut(s) 33
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 6 cut(s) 34, 141, 157, 170, 1429, 1464
PspPI GGNCC 4 cut(s) 4, 33, 156, 1534
PspPPI RGGWCCY 1 cut(s) 33
PsrI GAACNNNNNNTAC 2 cut(s) 242, 274
PstI CTGCAG 3 cut(s) 976, 985, 1988
PstNI CAGNNNCTG 2 cut(s) 329, 980
PsuI RGATCY 3 cut(s) 123, 139, 1462
PvuII CAGCTG 1 cut(s) 977
RsaI GTAC 9 cut(s) 251, 744, 860, 1053, 1441, 1613, 1683, 1763, 1886
RsaNI GTAC 9 cut(s) 250, 743, 859, 1052, 1440, 1612, 1682, 1762, 1885
RseI CAYNNNNRTG 3 cut(s) 50, 245, 1529
SacI GAGCTC 1 cut(s) 2105
SalI GTCGAC 1 cut(s) 236
SaqAI TTAA 6 cut(s) 353, 536, 729, 876, 884, 1943
Sau3AI GATC 9 cut(s) 123, 139, 183, 668, 1462, 1587, 1660, 1690, 2122
Sau96I GGNCC 4 cut(s) 4, 33, 156, 1534
ScaI AGTACT 2 cut(s) 860, 1053
SchI GAGTC 2 cut(s) 436, 985
ScrFI CCNGG 3 cut(s) 93, 166, 1372
SduI GDGCHC 2 cut(s) 269, 2105
SfaNI GCATC 4 cut(s) 51, 1021, 1222, 1880
SfcI CTRYAG 4 cut(s) 972, 981, 1131, 1984
SfuI TTCGAA 2 cut(s) 1789, 1861
SinI GGWCC 3 cut(s) 4, 33, 156
SmiMI CAYNNNNRTG 3 cut(s) 50, 245, 1529
SmlI CTYRAG 5 cut(s) 127, 262, 416, 546, 2045
SmoI CTYRAG 5 cut(s) 127, 262, 416, 546, 2045
SpeI ACTAGT 1 cut(s) 1576
SsiI CCGC 5 cut(s) 153, 942, 1096, 1847, 2019
SspMI CTAG 9 cut(s) 294, 432, 947, 1062, 1494, 1577, 1677, 1686, 2145
SstI GAGCTC 1 cut(s) 2105
StyD4I CCNGG 3 cut(s) 91, 164, 1370
StyI CCWWGG 7 cut(s) 27, 143, 405, 431, 823, 1323, 1771
TaaI ACNGT 9 cut(s) 103, 271, 646, 851, 1024, 1132, 1246, 1420, 1569
TaiI ACGT 2 cut(s) 26, 482
TaqI TCGA 9 cut(s) 83, 237, 600, 627, 770, 1455, 1789, 1861, 2153
TaqII GACCGA 1 cut(s) 1640
TatI WGTACW 5 cut(s) 249, 858, 1051, 1439, 1761
TauI GCSGC 3 cut(s) 944, 1099, 1849
TfiI GAWTC 7 cut(s) 118, 313, 629, 1147, 1741, 1863, 1923
Tru1I TTAA 6 cut(s) 353, 536, 729, 876, 884, 1943
Tru9I TTAA 6 cut(s) 353, 536, 729, 876, 884, 1943
TscAI CASTG 8 cut(s) 106, 247, 274, 391, 535, 1251, 1663, 1743
TseFI GTSAC 5 cut(s) 386, 475, 679, 967, 1024
Tsp45I GTSAC 5 cut(s) 386, 475, 679, 967, 1024
TspDTI ATGAA 3 cut(s) 894, 1969, 2074
TspGWI ACGGA 1 cut(s) 881
TspRI CASTG 8 cut(s) 106, 247, 274, 391, 535, 1251, 1663, 1743
VpaK11BI GGWCC 3 cut(s) 4, 33, 156
VspI ATTAAT 1 cut(s) 876
XapI RAATTY 5 cut(s) 705, 1335, 1857, 1994, 2052
XceI RCATGY 3 cut(s) 512, 1001, 1855
XmaJI CCTAGG 1 cut(s) 431
XmiI GTMKAC 4 cut(s) 237, 459, 648, 846
XmnI GAANNNNTTC 3 cut(s) 1339, 1379, 1922
XspI CTAG 9 cut(s) 294, 432, 947, 1062, 1494, 1577, 1677, 1686, 2145
ZraI GACGTC 1 cut(s) 24
ZrmI AGTACT 2 cut(s) 860, 1053
Zsp2I ATGCAT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.