Rh5BG115100

CSC1-like protein ERD4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011925
Physical Location & Seq
Forward (+)
10774633 .. 10779425
4793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG115100.1

Sequence Viewer

Length: 2187 bp
ATGGATCTCAGTTCGTTCCTGACGTCGTTAGGGACCTCGTTTGCTATATTTGTGATTCTGATGTTTCTGTTCGCGTGGCTTTCGAGAAAGCCCGGGAACGCGGTGGTGTACTATCCGAATCGGATCCTCAAGGGTTTGGATCCGTGGGAGGGCGGGTCCAAGACCCGGAACCCGTTTGCCTGGATCAAAGAGTCTCTGACTTCCACAGAGCAGGAACTTATAGCCATTTCTGGGGTTGACACTGCTGTCTACTTTGTCTTCTTAAGCACTGTGTTGGGAATACTGGTTTTGTCCAGCTTGATACTGCTACCAATGCTTCTGCCAGTTTCTGCCACTGACACTGGAGACACCACAACAAACACCACCACCAGCAACGGGACTTTCTCTGACCTTGACAAGTTATCCATAGGAAATGTCCAGGCGAAGAGTCCAAGGCTGTGGGCGTTTCTCCTAGGTGTCTACTGGGTTTCTTTTGTTACATATATCTTGTTGTGGAAGGCGTATAAACATGTCTCCGATCTGAGAGCCAATGCTTTAATGTCTCCTGATGTCAAGCCTGAACAATTTGCTGTTCTTGTTAGAGACATACCTGCTGTGCGTGAAGGTCCAAATAGAAAGGACCAGGTCGATTCATATTTCAGGGCCATCTACCCCGAGACCTATTACAAATCACTGATTGTTACAGACAATAAAGAGGTGAACAAACTTTGGAAAGAGTTAGAAGGGTTTAGGAAGAAGCTTGAACGTGCTGAAGCAGTTTATGCAGCATCCAAAACAACAGGCAGTCCAGAAGGAACAAGACCCACTAACAAAACTGGCTTCCTTGGTCTTTGTGGGGCAAAGGTTGATAGTATAGAGTACTACACTAAGAAGATTAATGAAACAATCCCAAAATTGGAAGCTGAACAAAAAGCCACTCTCAGAGAGAAGCAGCTAAATGCTGCTATAGTTTTCTTCACTAGTAGGGCGGCTGCAGCTTCTGCAGCACAGAGTCTGCATGCCAAAATGGGTGACAAATGGACAGTCACGGATGCTCCTGAACCTCGTCAAGTTCTTTGGCCTAATCTCAAAATCAAGTTTTTCCAGAGGCAAGTGCGGCAGTATGTTGTGTATATCATTGTGGCTCTGACCGTGGTGTTTTATATGATCCCAATTGCATTCATCTCTGCATTCACAACTCTGGATAATCTGGTGAAACTTATCCCATTTATCAAGCCAGTTGTGCATCAATCTGCGATCAAGACAGTGCTGGAGGCATTTCTTCCTCAGCTTGCACTTATAATCTTCTTAGCTTTGTTGCCGAAGCTACTTTTCGCTCTGTCTAAAGCCGAGGGAATTCCTTCTCAGAGCCATGCAGTACGGTCTGCCTCTGGAAAATATTTCTATTTCACTGTGTTCAATGTTTTTCTTGGAGTTACTATAGGTGGAACCTTGTTCAGTACATTCAAGGAAATCGAGAAGAATCCCAATCTCAATAAAATTATTACCTTACTGGCAACTAGCCTCCCAGGAAATGCAACTTACTTCGTCACCTTTGTGGCTCTGAAGTTTTTTATTGGCTATGGTCTTGAACTATCCCGATTAGTTCCTCTGATCATTTTCCATCTGAAAAGGAAGTATCTTTGTAAGACTGAAGGCGAGTTGAAAGCAGCTTGGCAACCTAGTGATCTGGGGTATGGGACTAGAGTTCCTGGTGATATGCTCATCATAACAATTGTTCTCTGCTACTCTGTTATTGCTCCACTGATCATTCCATTTGGTGTGCTGTACTTTGGCATCGGATGGCTTGTCCTTCGAAACCAGGCACTCAAAGTGTATTGTCCAGCGTATGAAAGTTATGGAAGGTTCTGGCCACACATGCAGATCCGGATTCTTGCAGCTCTGATATTGTACCAAGTTACCATGTTTGGTTTCTTTGGGGTAAAGAAATTTATATATGCACCGTTTGTGATCCCACTTCCTATCCTCTCCTTGATCTTCTGTTACACCTGTAGCAAGAAATTCTACCGATTTTTCAAAGACGCTGCTCTTGAAGTTGTGTCTCACGAACTGAAAGACATTCCTAACATGGAGCAAATTTATAAATCTTACCTCCCACCAAGCCTGAGCTCTGAGAAAGTACTACTAGATGATGATCAATTCGAAGATGCCAAGTCAAATGTGTCCAGAACAACATCATTTGCCTGA

Protein Analysis

728

Amino Acids

81.88

Weight (kDa)

9.36

Isoelectric Point (pI)

29.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 5 - 166 6.4e-34 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 188 - 355 1.3e-38 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 366 - 639 5.2e-79 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011925)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30360
fragaria_vesca FvH4_3g04980 FvH4_3g04980
malus_domestica MD05G1278500.v1.1 MD10G1257300.v1.1
prunus_persica Prupe.4G086300_v2.0.a1
pyrus_communis pycom05g25910 pycom10g21280 pycom10g21310 pycom10g21330
rosa_chinensis RchiOBHm_Chr5g0015001
rosa_laevigata RLG00000032159
rosa_multiflora Rmu_sc0004889.1_g000022
rosa_roxburghii Rroxscaffold_1G00061540
rosa_rugosa Rorug05G0022600 Rorug05G0022700
rosa_samantha Rh5AG117900 Rh5BG115100
rosa_wichuraiana Rw5G010160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1280, 2082
AasI GACNNNNNNGTC 1 cut(s) 245
AatII GACGTC 1 cut(s) 26
Acc36I ACCTGC 1 cut(s) 598
AccI GTMKAC 2 cut(s) 249, 459
AccII CGCG 2 cut(s) 74, 101
AccIII TCCGGA 1 cut(s) 1866
AciI CCGC 4 cut(s) 101, 153, 968, 1096
AclWI GGATC 9 cut(s) 12, 118, 131, 134, 147, 191, 1141, 1858, 1945
AcoI YGGCCR 1 cut(s) 1850
AcsI RAATTY 4 cut(s) 1335, 1928, 2000, 2076
AcuI CTGAAG 3 cut(s) 771, 1565, 1653
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 7 cut(s) 110, 860, 1359, 1441, 1769, 1892, 2121
AfiI CCNNNNNNNGG 5 cut(s) 149, 165, 231, 375, 895
AflII CTTAAG 1 cut(s) 262
AflIII ACRYGT 1 cut(s) 508
AgsI TTSAA 7 cut(s) 743, 1399, 1447, 1571, 1645, 2017, 2033
AhlI ACTAGT 1 cut(s) 959
AjnI CCWGG 6 cut(s) 179, 417, 621, 1507, 1690, 1800
AleI CACNNNNGTG 1 cut(s) 1535
Alw21I GWGCWC 1 cut(s) 2111
Alw26I GTCTC 7 cut(s) 198, 339, 517, 546, 576, 650, 2046
AlwI GGATC 9 cut(s) 12, 118, 131, 134, 147, 191, 1141, 1858, 1945
AlwNI CAGNNNCTG 3 cut(s) 329, 980, 994
Ama87I CYCGRG 2 cut(s) 92, 653
Aor13HI TCCGGA 1 cut(s) 1866
AoxI GGCC 3 cut(s) 642, 1058, 1850
ApeKI GCWGC 9 cut(s) 764, 931, 941, 971, 974, 983, 1649, 1877, 2024
ApoI RAATTY 4 cut(s) 1335, 1928, 2000, 2076
ArsI GACNNNNNNTTYG 2 cut(s) 1008, 1040
AseI ATTAAT 1 cut(s) 876
Asp700I GAANNNNTTC 1 cut(s) 1339
AspA2I CCTAGG 1 cut(s) 451
AspS9I GGNCC 5 cut(s) 33, 156, 605, 619, 642
AsuC2I CCSGG 3 cut(s) 93, 94, 166
AsuHPI GGTGA 5 cut(s) 709, 1022, 1204, 1522, 1706
AsuII TTCGAA 2 cut(s) 1795, 2142
AvaI CYCGRG 2 cut(s) 92, 653
AvaII GGWCC 4 cut(s) 33, 156, 605, 619
AvrII CCTAGG 1 cut(s) 451
BalI TGGCCA 1 cut(s) 1852
BamHI GGATCC 2 cut(s) 123, 139
BanII GRGCYC 1 cut(s) 2111
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 2111
BbvCI CCTCAGC 1 cut(s) 1266
BbvI GCAGC 9 cut(s) 776, 928, 943, 958, 986, 995, 1661, 1889, 2011
BccI CCATC 3 cut(s) 653, 1611, 1776
BciT130I CCWGG 6 cut(s) 181, 419, 623, 1509, 1692, 1802
BclI TGATCA 3 cut(s) 1593, 1746, 2134
BcnI CCSGG 3 cut(s) 93, 94, 166
BcoDI GTCTC 7 cut(s) 198, 339, 517, 546, 576, 650, 2046
BcuI ACTAGT 1 cut(s) 959
BfaI CTAG 6 cut(s) 452, 960, 1500, 1662, 1683, 2126
BfmI CTRYAG 5 cut(s) 945, 972, 981, 1419, 1990
BfrI CTTAAG 1 cut(s) 262
BfuAI ACCTGC 1 cut(s) 598
BlnI CCTAGG 1 cut(s) 451
BmcAI AGTACT 2 cut(s) 860, 2121
Bme1390I CCNGG 9 cut(s) 93, 94, 166, 181, 419, 623, 1509, 1692, 1802
Bme18I GGWCC 4 cut(s) 33, 156, 605, 619
BmeT110I CYCGRG 2 cut(s) 92, 653
BmgT120I GGNCC 5 cut(s) 33, 156, 605, 619, 642
BmiI GGNNCC 6 cut(s) 34, 125, 141, 157, 170, 1429
BmrFI CCNGG 9 cut(s) 93, 94, 166, 181, 419, 623, 1509, 1692, 1802
BmrI ACTGGG 1 cut(s) 472
BmsI GCATC 5 cut(s) 776, 1021, 1234, 1785, 2137
BmuI ACTGGG 1 cut(s) 472
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 2 cut(s) 363, 1271
Bpu10I CCTNAGC 2 cut(s) 1266, 2105
Bpu14I TTCGAA 2 cut(s) 1795, 2142
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 3 cut(s) 93, 94, 166
BsaBI GATNNNNATC 1 cut(s) 2133
BsaHI GRCGYC 1 cut(s) 23
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 8 cut(s) 92, 143, 431, 451, 823, 1131, 1329, 1507
BsaWI WCCGGW 1 cut(s) 1866
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 1018, 1048
Bsc4I CCNNNNNNNGG 5 cut(s) 149, 165, 231, 375, 895
Bse1I ACTGG 7 cut(s) 288, 323, 346, 467, 820, 1217, 1497
Bse8I GATNNNNATC 1 cut(s) 2133
BseAI TCCGGA 1 cut(s) 1866
BseBI CCWGG 6 cut(s) 181, 419, 623, 1509, 1692, 1802
BseDI CCNNGG 8 cut(s) 92, 143, 431, 451, 823, 1131, 1329, 1507
BseGI GGATG 3 cut(s) 767, 1036, 1787
BseJI GATNNNNATC 1 cut(s) 2133
BseLI CCNNNNNNNGG 5 cut(s) 149, 165, 231, 375, 895
BseMII CTCAG 7 cut(s) 22, 512, 934, 1280, 1358, 2096, 2103
BseNI ACTGG 7 cut(s) 288, 323, 346, 467, 820, 1217, 1497
BseXI GCAGC 9 cut(s) 776, 928, 943, 958, 986, 995, 1661, 1889, 2011
Bsh1236I CGCG 2 cut(s) 74, 101
BshFI GGCC 3 cut(s) 644, 1060, 1852
BsiHKAI GWGCWC 1 cut(s) 2111
BsiHKCI CYCGRG 2 cut(s) 92, 653
BsiSI CCGG 3 cut(s) 93, 166, 1867
BslFI GGGAC 3 cut(s) 46, 391, 1693
BslI CCNNNNNNNGG 5 cut(s) 149, 165, 231, 375, 895
BsmAI GTCTC 7 cut(s) 198, 339, 517, 546, 576, 650, 2046
BsmFI GGGAC 3 cut(s) 46, 391, 1693
BsmI GAATGC 2 cut(s) 1157, 1169
BsnI GGCC 3 cut(s) 644, 1060, 1852
Bso31I GGTCTC 1 cut(s) 650
BsoBI CYCGRG 2 cut(s) 92, 653
Bsp119I TTCGAA 2 cut(s) 1795, 2142
Bsp1286I GDGCHC 1 cut(s) 2111
Bsp13I TCCGGA 1 cut(s) 1866
BspACI CCGC 4 cut(s) 101, 153, 968, 1096
BspANI GGCC 3 cut(s) 644, 1060, 1852
BspCNI CTCAG 7 cut(s) 21, 513, 933, 1279, 1357, 2097, 2104
BspEI TCCGGA 1 cut(s) 1866
BspFNI CGCG 2 cut(s) 74, 101
BspLI GGNNCC 6 cut(s) 34, 125, 141, 157, 170, 1429
BspMAI CTGCAG 2 cut(s) 976, 985
BspMI ACCTGC 1 cut(s) 598
BspPI GGATC 9 cut(s) 12, 118, 131, 134, 147, 191, 1141, 1858, 1945
BspT104I TTCGAA 2 cut(s) 1795, 2142
BspTI CTTAAG 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 650
BsrI ACTGG 7 cut(s) 288, 323, 346, 467, 820, 1217, 1497
BssECI CCNNGG 8 cut(s) 92, 143, 431, 451, 823, 1131, 1329, 1507
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 3 cut(s) 431, 451, 823
Bst2UI CCWGG 6 cut(s) 181, 419, 623, 1509, 1692, 1802
Bst4CI ACNGT 7 cut(s) 271, 1024, 1132, 1246, 1362, 1393, 1944
Bst6I CTCTTC 1 cut(s) 419
BstACI GRCGYC 1 cut(s) 23
BstAFI CTTAAG 1 cut(s) 262
BstAPI GCANNNNNTGC 2 cut(s) 761, 980
BstBI TTCGAA 2 cut(s) 1795, 2142
BstC8I GCNNGC 2 cut(s) 999, 1272
BstDEI CTNAG 9 cut(s) 8, 521, 867, 920, 1266, 1288, 1344, 2105, 2112
BstDSI CCRYGG 2 cut(s) 143, 1131
BstF5I GGATG 3 cut(s) 767, 1036, 1787
BstFNI CGCG 2 cut(s) 74, 101
BstMAI GTCTC 7 cut(s) 198, 339, 517, 546, 576, 650, 2046
BstMWI GCNNNNNNNGC 8 cut(s) 313, 761, 974, 980, 983, 1096, 1222, 1858
BstNI CCWGG 6 cut(s) 181, 419, 623, 1509, 1692, 1802
BstNSI RCATGY 3 cut(s) 512, 1001, 1861
BstSCI CCNGG 9 cut(s) 91, 92, 164, 179, 417, 621, 1507, 1690, 1800
BstSFI CTRYAG 5 cut(s) 945, 972, 981, 1419, 1990
BstUI CGCG 2 cut(s) 74, 101
BstV1I GCAGC 9 cut(s) 776, 928, 943, 958, 986, 995, 1661, 1889, 2011
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 4 cut(s) 4, 123, 139, 1863
BstXI CCANNNNNNTGG 1 cut(s) 438
BstYI RGATCY 4 cut(s) 4, 123, 139, 1863
BsuRI GGCC 3 cut(s) 644, 1060, 1852
BtgI CCRYGG 2 cut(s) 143, 1131
BtsCI GGATG 3 cut(s) 767, 1036, 1787
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 8 cut(s) 240, 267, 333, 339, 671, 1251, 1389, 1742
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 2 cut(s) 999, 1272
CaiI CAGNNNCTG 3 cut(s) 329, 980, 994
Cfr13I GGNCC 5 cut(s) 33, 156, 605, 619, 642
Cfr9I CCCGGG 1 cut(s) 92
CseI GACGC 1 cut(s) 2030
CsiI ACCWGGT 1 cut(s) 621
Csp6I GTAC 7 cut(s) 109, 859, 1358, 1440, 1768, 1891, 2120
CviAII CATG 6 cut(s) 509, 998, 1352, 1858, 1903, 2068
CviQI GTAC 7 cut(s) 109, 859, 1358, 1440, 1768, 1891, 2120
DdeI CTNAG 9 cut(s) 8, 521, 867, 920, 1266, 1288, 1344, 2105, 2112
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 1850
Eam1104I CTCTTC 1 cut(s) 419
EarI CTCTTC 1 cut(s) 419
Ecl136II GAGCTC 1 cut(s) 2109
Eco130I CCWWGG 3 cut(s) 431, 451, 823
Eco24I GRGCYC 1 cut(s) 2111
Eco31I GGTCTC 1 cut(s) 650
Eco47I GGWCC 4 cut(s) 33, 156, 605, 619
Eco53kI GAGCTC 1 cut(s) 2109
Eco57I CTGAAG 3 cut(s) 771, 1565, 1653
Eco88I CYCGRG 2 cut(s) 92, 653
EcoICRI GAGCTC 1 cut(s) 2109
EcoO109I RGGNCCY 1 cut(s) 33
EcoRI GAATTC 1 cut(s) 1335
EcoRII CCWGG 6 cut(s) 179, 417, 621, 1507, 1690, 1800
EcoT14I CCWWGG 3 cut(s) 431, 451, 823
EcoT38I GRGCYC 1 cut(s) 2111
ErhI CCWWGG 3 cut(s) 431, 451, 823
FaeI CATG 6 cut(s) 512, 1001, 1355, 1861, 1906, 2071
FaqI GGGAC 3 cut(s) 46, 391, 1693
FatI CATG 6 cut(s) 508, 997, 1351, 1857, 1902, 2067
FauI CCCGC 1 cut(s) 146
FbaI TGATCA 3 cut(s) 1593, 1746, 2134
FblI GTMKAC 2 cut(s) 249, 459
FokI GGATG 3 cut(s) 754, 1043, 1794
FriOI GRGCYC 1 cut(s) 2111
FspBI CTAG 6 cut(s) 452, 960, 1500, 1662, 1683, 2126
GsuI CTGGAG 2 cut(s) 363, 1271
HaeIII GGCC 3 cut(s) 644, 1060, 1852
HapII CCGG 3 cut(s) 93, 166, 1867
HgaI GACGC 1 cut(s) 2030
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 6 cut(s) 512, 1001, 1355, 1861, 1906, 2071
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 8 cut(s) 55, 118, 191, 427, 629, 991, 1462, 1870
HpaII CCGG 3 cut(s) 93, 166, 1867
HphI GGTGA 5 cut(s) 709, 1022, 1204, 1522, 1706
Hpy166II GTNNAC 5 cut(s) 109, 238, 250, 460, 700
Hpy8I GTNNAC 5 cut(s) 109, 238, 250, 460, 700
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 8 cut(s) 490, 596, 716, 785, 1350, 1628, 1802, 1836
HpyCH4III ACNGT 7 cut(s) 271, 1024, 1132, 1246, 1362, 1393, 1944
HpyCH4IV ACGT 2 cut(s) 23, 745
HpyF10VI GCNNNNNNNGC 8 cut(s) 313, 761, 974, 980, 983, 1096, 1222, 1858
HpyF3I CTNAG 9 cut(s) 8, 521, 867, 920, 1266, 1288, 1344, 2105, 2112
HpySE526I ACGT 2 cut(s) 23, 745
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 6 cut(s) 512, 1001, 1355, 1861, 1906, 2071
Kpn2I TCCGGA 1 cut(s) 1866
Ksp22I TGATCA 3 cut(s) 1593, 1746, 2134
LmnI GCTCC 3 cut(s) 1039, 1744, 2071
Lsp1109I GCAGC 9 cut(s) 776, 928, 943, 958, 986, 995, 1661, 1889, 2011
LweI GCATC 5 cut(s) 776, 1021, 1234, 1785, 2137
MabI ACCWGGT 1 cut(s) 621
MaeI CTAG 6 cut(s) 452, 960, 1500, 1662, 1683, 2126
MaeII ACGT 2 cut(s) 23, 745
MaeIII GTNAC 8 cut(s) 475, 679, 1010, 1024, 1414, 1528, 1897, 1982
MfeI CAATTG 2 cut(s) 1152, 1713
MflI RGATCY 4 cut(s) 4, 123, 139, 1863
MhlI GDGCHC 1 cut(s) 2111
MlsI TGGCCA 1 cut(s) 1852
MluNI TGGCCA 1 cut(s) 1852
MlyI GAGTC 3 cut(s) 200, 436, 1000
Mox20I TGGCCA 1 cut(s) 1852
MroI TCCGGA 1 cut(s) 1866
MroXI GAANNNNTTC 1 cut(s) 1339
MscI TGGCCA 1 cut(s) 1852
MseI TTAA 3 cut(s) 263, 536, 876
MslI CAYNNNNRTG 1 cut(s) 1535
Msp20I TGGCCA 1 cut(s) 1852
MspCI CTTAAG 1 cut(s) 262
MspI CCGG 3 cut(s) 93, 166, 1867
MspR9I CCNGG 9 cut(s) 93, 94, 166, 181, 419, 623, 1509, 1692, 1802
MunI CAATTG 2 cut(s) 1152, 1713
Mva1269I GAATGC 2 cut(s) 1157, 1169
MvaI CCWGG 6 cut(s) 181, 419, 623, 1509, 1692, 1802
MvnI CGCG 2 cut(s) 74, 101
MwoI GCNNNNNNNGC 8 cut(s) 313, 761, 974, 980, 983, 1096, 1222, 1858
NciI CCSGG 3 cut(s) 93, 94, 166
NlaIII CATG 6 cut(s) 512, 1001, 1355, 1861, 1906, 2071
NlaIV GGNNCC 6 cut(s) 34, 125, 141, 157, 170, 1429
NmeAIII GCCGAG 1 cut(s) 1354
NmuCI GTSAC 3 cut(s) 1010, 1024, 1528
NspI RCATGY 3 cut(s) 512, 1001, 1861
NspV TTCGAA 2 cut(s) 1795, 2142
OliI CACNNNNGTG 1 cut(s) 1535
PaeI GCATGC 1 cut(s) 1001
PciI ACATGT 1 cut(s) 508
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 2 cut(s) 1157, 1169
PdmI GAANNNNTTC 1 cut(s) 1339
PfeI GAWTC 5 cut(s) 55, 118, 629, 1462, 1870
PflFI GACNNNGTC 1 cut(s) 623
PleI GAGTC 3 cut(s) 199, 435, 999
PpsI GAGTC 3 cut(s) 199, 435, 999
PpuMI RGGWCCY 1 cut(s) 33
PscI ACATGT 1 cut(s) 508
PshBI ATTAAT 1 cut(s) 876
PsiI TTATAA 2 cut(s) 1280, 2082
Psp124BI GAGCTC 1 cut(s) 2111
Psp5II RGGWCCY 1 cut(s) 33
Psp6I CCWGG 6 cut(s) 179, 417, 621, 1507, 1690, 1800
PspGI CCWGG 6 cut(s) 179, 417, 621, 1507, 1690, 1800
PspN4I GGNNCC 6 cut(s) 34, 125, 141, 157, 170, 1429
PspPI GGNCC 5 cut(s) 33, 156, 605, 619, 642
PspPPI RGGWCCY 1 cut(s) 33
PstI CTGCAG 2 cut(s) 976, 985
PstNI CAGNNNCTG 3 cut(s) 329, 980, 994
PsuI RGATCY 4 cut(s) 4, 123, 139, 1863
PsyI GACNNNGTC 1 cut(s) 623
RsaI GTAC 7 cut(s) 110, 860, 1359, 1441, 1769, 1892, 2121
RsaNI GTAC 7 cut(s) 109, 859, 1358, 1440, 1768, 1891, 2120
RseI CAYNNNNRTG 1 cut(s) 1535
SacI GAGCTC 1 cut(s) 2111
SaqAI TTAA 3 cut(s) 263, 536, 876
Sau96I GGNCC 5 cut(s) 33, 156, 605, 619, 642
ScaI AGTACT 2 cut(s) 860, 2121
SchI GAGTC 3 cut(s) 200, 436, 1000
ScrFI CCNGG 9 cut(s) 93, 94, 166, 181, 419, 623, 1509, 1692, 1802
SduI GDGCHC 1 cut(s) 2111
SexAI ACCWGGT 1 cut(s) 621
SfaNI GCATC 5 cut(s) 776, 1021, 1234, 1785, 2137
SfcI CTRYAG 5 cut(s) 945, 972, 981, 1419, 1990
SfuI TTCGAA 2 cut(s) 1795, 2142
SinI GGWCC 4 cut(s) 33, 156, 605, 619
SmaI CCCGGG 1 cut(s) 94
SmiMI CAYNNNNRTG 1 cut(s) 1535
SmlI CTYRAG 2 cut(s) 128, 262
SmoI CTYRAG 2 cut(s) 128, 262
SpeI ACTAGT 1 cut(s) 959
SphI GCATGC 1 cut(s) 1001
SsiI CCGC 4 cut(s) 101, 153, 968, 1096
SspI AATATT 1 cut(s) 1379
SspMI CTAG 6 cut(s) 452, 960, 1500, 1662, 1683, 2126
SstI GAGCTC 1 cut(s) 2111
StyD4I CCNGG 9 cut(s) 91, 92, 164, 179, 417, 621, 1507, 1690, 1800
StyI CCWWGG 3 cut(s) 431, 451, 823
TaaI ACNGT 7 cut(s) 271, 1024, 1132, 1246, 1362, 1393, 1944
TaiI ACGT 2 cut(s) 26, 748
TaqI TCGA 5 cut(s) 83, 627, 1455, 1795, 2142
TatI WGTACW 5 cut(s) 108, 858, 1439, 1767, 2119
TauI GCSGC 2 cut(s) 971, 1099
TfiI GAWTC 5 cut(s) 55, 118, 629, 1462, 1870
Tru1I TTAA 3 cut(s) 263, 536, 876
Tru9I TTAA 3 cut(s) 263, 536, 876
TscAI CASTG 8 cut(s) 247, 274, 340, 346, 678, 1251, 1396, 1749
TseFI GTSAC 3 cut(s) 1010, 1024, 1528
TseI GCWGC 9 cut(s) 764, 931, 941, 971, 974, 983, 1649, 1877, 2024
Tsp45I GTSAC 3 cut(s) 1010, 1024, 1528
TspDTI ATGAA 4 cut(s) 621, 894, 1150, 1845
TspGWI ACGGA 2 cut(s) 132, 1043
TspMI CCCGGG 1 cut(s) 92
TspRI CASTG 8 cut(s) 247, 274, 340, 346, 678, 1251, 1396, 1749
Tth111I GACNNNGTC 1 cut(s) 623
Vha464I CTTAAG 1 cut(s) 262
VpaK11BI GGWCC 4 cut(s) 33, 156, 605, 619
VspI ATTAAT 1 cut(s) 876
XapI RAATTY 4 cut(s) 1335, 1928, 2000, 2076
XceI RCATGY 3 cut(s) 512, 1001, 1861
XmaI CCCGGG 1 cut(s) 92
XmaJI CCTAGG 1 cut(s) 451
XmiI GTMKAC 2 cut(s) 249, 459
XmnI GAANNNNTTC 1 cut(s) 1339
XspI CTAG 6 cut(s) 452, 960, 1500, 1662, 1683, 2126
ZraI GACGTC 1 cut(s) 24
ZrmI AGTACT 2 cut(s) 860, 2121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.