RchiOBHm_Chr5g0015001

CSC1-like protein ERD4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011925
Physical Location & Seq
Forward (+)
10381571 .. 10386858
5288 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29543

Sequence Viewer

Length: 2184 bp
ATGGATCTCAGTTCGTTCTTGACATCGTTAGGGACCTCGTTTGCGATATTTGTGATTCTGATGTTTCTGTTCGCTTGGCTTTCGGGAAAGCCCGGGAACGCGGTGGTGTACTATCCGAATCGGATCCTGAAGGGTTTGGATCCGTGGGAGGGCGGGTCCAAGACCCGGAACCCGTTTGCCTGGATCAAAGAGGCTCTGACTTCCACAGAGCAGGAAGTTATAGCCATTTCTGGAGTTGACACTGCTGTCTACTTTGTCTTCTTAAGCACTGTGTTGGGAATACTGGTTTTGTCCAGCTTGATACTGCTACCAATGCTCCTGCCAGTTTCTGTCACTGACACTGGAGACACCACAACAAACACCACCACCAGCCACGGGACTTTCTCTGACCTTGACAAGTTATCCATAGGAAATGTCCAGGCGAAGAGTCCAAGGCTGTGGGCGTTTCTCCTAGGTGTCTACTGGGTTTCTTTTGTTACATATATCTTGTTGTGGAAGGCGTATAAACATGTCTCCGATCTGAGAGCCAATGCTTTAATGTCTCCTGATGTCAAGCCTGAACAATTTGCTGTTCTTGTTAGAGACATACCTGCTGTGTCTGAAGGTCCAAATAGAAAGGACCAGGTCGATTCATATTTCAGGGCCATCTACCCCGAGACCTATTACAAATCACTGATTGCTACAGACAATAAAGTGGTGAACAAACTTTGGAAAGAGTTAGAAGGGTTTAGGAAGAAGCTTGAACGTGCTGAAGCAGTTTATGCAACATCCAAAACAACAGGCAGTCCAGGAGGAACAAGACCCACTAACAAAACTGGCTTCCTTGGTCTTTGTGGGGCAAAGGTTGATAGTATAGAGTACTACACTGAGAAGATTAATGAAACAATCCCAAAATTGGAAGCTGAACAAAAAGCCACTCTCAGAGAGAAGCAGCTAAATGCTGCTATAGTTTTCTTCACTAGTAGGGCGGCTGCAGCGGCTGCAGCACAGAGTCTACATTCCCAAATGGTTGACAAATGGACAGTCACGGATGCTCCTGAACCTCGTCAAGTTCTTTGGCCTAATCTCAAAATAAAGTTTTTCCAGAGGCAAGTGCGGCAGTATGTTGTGTATATCATTGTGGCTCTGACCGTGGTGTTTTATATGATCCCAATTACATTCATCTCTGCATTCACAACTCTGGATAATCTGGTGAAACTTATCCCATTTATCAAGCCAGTTGTGCGTCAATCTGCGCTCAAGACAGTGCTGGAGGCATTTCTTCCTCAGCTTGCACTTATAATCTTCTTAGCTTTGTTGCCGAAGCTACTTTTCGCTCTGTCTAAAGCCGAGGGAATTCCTTCTCAGAGCCATGCAGTACGGTCTGCCTCTGGAAAATATTTCTATTTCACTGTGTTCAATGTTTTTCTTGGAGTTACTATAGGTGGAACCTTGTTCAGTACATTCAAGGAAATCCAGAAGAATCCCAGTCTCGAGAAAATTATTACCTTACTGGCAAATAGCCTCCCAGGAAATGCAACTTACTTCGTCACCTTTGTGGCTCTGAAGTTTTTTATTGGCTATGGTCTTGAACTATCCCGATTAGTTCCTCTGATCATTTTCCATCTGAAAAGGAAGTATCTTTGTAAGACTGAAGGCGAGTTGAAAGCAGCTTGGCAACCTAGTGATCTGGGGTATGGGACTAGAGTTCCTGGTGATTTGCTCATCATAACAATTGTTCTCTGCTACTCTGTTATTGCTCCACTGATCATTCCATTTGGTGTGCTGTACTTTGGCATCGGATGGCTTGTCCTTCGAAACCAGGCACTCAAAGTGTATTGTCCAGCGTATGAAAGTTATGGAAGGTTCTGGCCACACATGCAGGTCCGGATTCTTGCAGCTCTGCTATTGTACCAAGTTACCATGTTTGGTTTCTTTGGGGTAAAGAAATTTGTATATGCACCGTTTGTGATCCCACTTCCTATCCTCTCCTTGATCTTCTGTTACGTCTGTAGCAAGAAATTCTATCGATTTTTCAAAGATGCTGCTCTTGAAGTTGCGTCTCACGAACTGAAAGACATTCCTAACATGGAGCAAATTTATAAATCTTACCTCCCACCAAGCCTGAGCTCTGAGAAAGTACTAGATGATGATCAATTCGAAGATGCCAAGTCAAATGTGTCCAGAACAACATCATTTGCCTGA

Protein Analysis

727

Amino Acids

81.53

Weight (kDa)

9.31

Isoelectric Point (pI)

28.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 5 - 166 4.5e-34 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 188 - 355 1.5e-38 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 366 - 639 7.8e-79 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011925)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30360
fragaria_vesca FvH4_3g04980 FvH4_3g04980
malus_domestica MD05G1278500.v1.1 MD10G1257300.v1.1
prunus_persica Prupe.4G086300_v2.0.a1
pyrus_communis pycom05g25910 pycom10g21280 pycom10g21310 pycom10g21330
rosa_chinensis RchiOBHm_Chr5g0015001
rosa_laevigata RLG00000032159
rosa_multiflora Rmu_sc0004889.1_g000022
rosa_roxburghii Rroxscaffold_1G00061540
rosa_rugosa Rorug05G0022600 Rorug05G0022700
rosa_samantha Rh5AG117900 Rh5BG115100
rosa_wichuraiana Rw5G010160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1280, 2082
AasI GACNNNNNNGTC 1 cut(s) 245
Acc36I ACCTGC 2 cut(s) 598, 1852
AccI GTMKAC 3 cut(s) 249, 459, 994
AccII CGCG 1 cut(s) 101
AccIII TCCGGA 1 cut(s) 1866
AciI CCGC 5 cut(s) 101, 153, 968, 977, 1096
AclWI GGATC 8 cut(s) 12, 118, 131, 134, 147, 191, 1141, 1945
AcoI YGGCCR 1 cut(s) 1850
AcsI RAATTY 4 cut(s) 1335, 1928, 2000, 2076
AcuI CTGAAG 5 cut(s) 149, 621, 771, 1565, 1653
AfaI GTAC 7 cut(s) 110, 860, 1359, 1441, 1769, 1892, 2121
AfiI CCNNNNNNNGG 4 cut(s) 149, 165, 375, 895
AflII CTTAAG 1 cut(s) 262
AflIII ACRYGT 1 cut(s) 508
AgsI TTSAA 7 cut(s) 743, 1399, 1447, 1571, 1645, 2017, 2033
AhlI ACTAGT 1 cut(s) 959
AjnI CCWGG 7 cut(s) 179, 417, 621, 787, 1507, 1690, 1800
AleI CACNNNNGTG 1 cut(s) 1535
Alw21I GWGCWC 1 cut(s) 2111
Alw26I GTCTC 7 cut(s) 339, 517, 546, 576, 650, 1475, 2046
AlwI GGATC 8 cut(s) 12, 118, 131, 134, 147, 191, 1141, 1945
AlwNI CAGNNNCTG 2 cut(s) 329, 980
Ama87I CYCGRG 3 cut(s) 92, 653, 1472
Aor13HI TCCGGA 1 cut(s) 1866
AoxI GGCC 3 cut(s) 642, 1058, 1850
ApeKI GCWGC 9 cut(s) 931, 941, 971, 974, 980, 983, 1649, 1877, 2024
ApoI RAATTY 4 cut(s) 1335, 1928, 2000, 2076
ArsI GACNNNNNNTTYG 2 cut(s) 1008, 1040
AseI ATTAAT 1 cut(s) 876
Asp700I GAANNNNTTC 1 cut(s) 1339
AspA2I CCTAGG 1 cut(s) 451
AspLEI GCGC 1 cut(s) 1237
AspS9I GGNCC 6 cut(s) 33, 156, 605, 619, 642, 1864
AsuC2I CCSGG 3 cut(s) 93, 94, 166
AsuHPI GGTGA 4 cut(s) 709, 1204, 1522, 1706
AsuII TTCGAA 2 cut(s) 1795, 2139
AvaI CYCGRG 3 cut(s) 92, 653, 1472
AvaII GGWCC 5 cut(s) 33, 156, 605, 619, 1864
AvrII CCTAGG 1 cut(s) 451
BalI TGGCCA 1 cut(s) 1852
BamHI GGATCC 2 cut(s) 123, 139
BanII GRGCYC 1 cut(s) 2111
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 2111
BbvCI CCTCAGC 1 cut(s) 1266
BbvI GCAGC 9 cut(s) 928, 943, 958, 967, 986, 995, 1661, 1889, 2011
BccI CCATC 3 cut(s) 653, 1611, 1776
BciT130I CCWGG 7 cut(s) 181, 419, 623, 789, 1509, 1692, 1802
BclI TGATCA 3 cut(s) 1593, 1746, 2131
BcnI CCSGG 3 cut(s) 93, 94, 166
BcoDI GTCTC 7 cut(s) 339, 517, 546, 576, 650, 1475, 2046
BcuI ACTAGT 1 cut(s) 959
BfaI CTAG 5 cut(s) 452, 960, 1662, 1683, 2123
BfmI CTRYAG 6 cut(s) 681, 945, 972, 981, 1419, 1990
BfrI CTTAAG 1 cut(s) 262
BfuAI ACCTGC 2 cut(s) 598, 1852
BlnI CCTAGG 1 cut(s) 451
BmcAI AGTACT 2 cut(s) 860, 2121
Bme18I GGWCC 5 cut(s) 33, 156, 605, 619, 1864
BmeT110I CYCGRG 3 cut(s) 92, 653, 1472
BmgT120I GGNCC 6 cut(s) 33, 156, 605, 619, 642, 1864
BmiI GGNNCC 6 cut(s) 34, 125, 141, 157, 170, 1429
BmrI ACTGGG 2 cut(s) 472, 1461
BmsI GCATC 4 cut(s) 1021, 1785, 2011, 2134
BmuI ACTGGG 2 cut(s) 472, 1461
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 3 cut(s) 252, 363, 1271
Bpu10I CCTNAGC 2 cut(s) 1266, 2105
Bpu14I TTCGAA 2 cut(s) 1795, 2139
BpuEI CTTGAG 1 cut(s) 1223
BpuMI CCSGG 3 cut(s) 93, 94, 166
Bsa29I ATCGAT 1 cut(s) 2008
BsaBI GATNNNNATC 1 cut(s) 2130
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 9 cut(s) 92, 143, 373, 431, 451, 823, 1131, 1329, 1507
BsaWI WCCGGW 1 cut(s) 1866
BsaXI ACNNNNNCTCC 6 cut(s) 140, 170, 300, 330, 1018, 1048
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 165, 375, 895
Bse1I ACTGG 8 cut(s) 288, 323, 346, 467, 820, 1217, 1467, 1497
Bse8I GATNNNNATC 1 cut(s) 2130
BseAI TCCGGA 1 cut(s) 1866
BseBI CCWGG 7 cut(s) 181, 419, 623, 789, 1509, 1692, 1802
BseCI ATCGAT 1 cut(s) 2008
BseDI CCNNGG 9 cut(s) 92, 143, 373, 431, 451, 823, 1131, 1329, 1507
BseGI GGATG 3 cut(s) 767, 1036, 1787
BseJI GATNNNNATC 1 cut(s) 2130
BseLI CCNNNNNNNGG 4 cut(s) 149, 165, 375, 895
BseMII CTCAG 8 cut(s) 22, 512, 858, 934, 1280, 1358, 2096, 2103
BseNI ACTGG 8 cut(s) 288, 323, 346, 467, 820, 1217, 1467, 1497
BseXI GCAGC 9 cut(s) 928, 943, 958, 967, 986, 995, 1661, 1889, 2011
Bsh1236I CGCG 1 cut(s) 101
BshFI GGCC 3 cut(s) 644, 1060, 1852
BshVI ATCGAT 1 cut(s) 2008
BsiHKAI GWGCWC 1 cut(s) 2111
BsiHKCI CYCGRG 3 cut(s) 92, 653, 1472
BsiSI CCGG 3 cut(s) 93, 166, 1867
BslFI GGGAC 3 cut(s) 46, 391, 1693
BslI CCNNNNNNNGG 4 cut(s) 149, 165, 375, 895
BsmAI GTCTC 7 cut(s) 339, 517, 546, 576, 650, 1475, 2046
BsmBI CGTCTC 1 cut(s) 2046
BsmFI GGGAC 3 cut(s) 46, 391, 1693
BsmI GAATGC 1 cut(s) 1169
BsnI GGCC 3 cut(s) 644, 1060, 1852
Bso31I GGTCTC 1 cut(s) 650
BsoBI CYCGRG 3 cut(s) 92, 653, 1472
Bsp119I TTCGAA 2 cut(s) 1795, 2139
Bsp1286I GDGCHC 1 cut(s) 2111
Bsp13I TCCGGA 1 cut(s) 1866
BspACI CCGC 5 cut(s) 101, 153, 968, 977, 1096
BspANI GGCC 3 cut(s) 644, 1060, 1852
BspCNI CTCAG 8 cut(s) 21, 513, 859, 933, 1279, 1357, 2097, 2104
BspDI ATCGAT 1 cut(s) 2008
BspEI TCCGGA 1 cut(s) 1866
BspFNI CGCG 1 cut(s) 101
BspLI GGNNCC 6 cut(s) 34, 125, 141, 157, 170, 1429
BspMAI CTGCAG 2 cut(s) 976, 985
BspMI ACCTGC 2 cut(s) 598, 1852
BspPI GGATC 8 cut(s) 12, 118, 131, 134, 147, 191, 1141, 1945
BspT104I TTCGAA 2 cut(s) 1795, 2139
BspTI CTTAAG 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 650
BsrI ACTGG 8 cut(s) 288, 323, 346, 467, 820, 1217, 1467, 1497
BssECI CCNNGG 9 cut(s) 92, 143, 373, 431, 451, 823, 1131, 1329, 1507
BssT1I CCWWGG 3 cut(s) 431, 451, 823
Bst2UI CCWGG 7 cut(s) 181, 419, 623, 789, 1509, 1692, 1802
Bst4CI ACNGT 7 cut(s) 271, 1024, 1132, 1246, 1362, 1393, 1944
Bst6I CTCTTC 1 cut(s) 419
BstAFI CTTAAG 1 cut(s) 262
BstAPI GCANNNNNTGC 2 cut(s) 761, 980
BstBI TTCGAA 2 cut(s) 1795, 2139
BstC8I GCNNGC 1 cut(s) 1272
BstDEI CTNAG 9 cut(s) 8, 521, 867, 920, 1266, 1288, 1344, 2105, 2112
BstDSI CCRYGG 3 cut(s) 143, 373, 1131
BstF5I GGATG 3 cut(s) 767, 1036, 1787
BstFNI CGCG 1 cut(s) 101
BstHHI GCGC 1 cut(s) 1237
BstMAI GTCTC 7 cut(s) 339, 517, 546, 576, 650, 1475, 2046
BstMWI GCNNNNNNNGC 9 cut(s) 313, 761, 974, 977, 980, 983, 1096, 1222, 1858
BstNI CCWGG 7 cut(s) 181, 419, 623, 789, 1509, 1692, 1802
BstNSI RCATGY 2 cut(s) 512, 1861
BstSFI CTRYAG 6 cut(s) 681, 945, 972, 981, 1419, 1990
BstUI CGCG 1 cut(s) 101
BstV1I GCAGC 9 cut(s) 928, 943, 958, 967, 986, 995, 1661, 1889, 2011
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 3 cut(s) 4, 123, 139
BstXI CCANNNNNNTGG 1 cut(s) 438
BstYI RGATCY 3 cut(s) 4, 123, 139
Bsu15I ATCGAT 1 cut(s) 2008
BsuRI GGCC 3 cut(s) 644, 1060, 1852
BsuTUI ATCGAT 1 cut(s) 2008
BtgI CCRYGG 3 cut(s) 143, 373, 1131
BtsCI GGATG 3 cut(s) 767, 1036, 1787
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 9 cut(s) 240, 267, 333, 339, 671, 864, 1251, 1389, 1742
BveI ACCTGC 2 cut(s) 598, 1852
Cac8I GCNNGC 1 cut(s) 1272
CaiI CAGNNNCTG 2 cut(s) 329, 980
CfoI GCGC 1 cut(s) 1237
Cfr13I GGNCC 6 cut(s) 33, 156, 605, 619, 642, 1864
Cfr9I CCCGGG 1 cut(s) 92
ClaI ATCGAT 1 cut(s) 2008
CseI GACGC 2 cut(s) 1214, 2028
CsiI ACCWGGT 1 cut(s) 621
Csp6I GTAC 7 cut(s) 109, 859, 1358, 1440, 1768, 1891, 2120
CviAII CATG 5 cut(s) 509, 1352, 1858, 1903, 2068
CviQI GTAC 7 cut(s) 109, 859, 1358, 1440, 1768, 1891, 2120
DdeI CTNAG 9 cut(s) 8, 521, 867, 920, 1266, 1288, 1344, 2105, 2112
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 1850
Eam1104I CTCTTC 1 cut(s) 419
EarI CTCTTC 1 cut(s) 419
Ecl136II GAGCTC 1 cut(s) 2109
Eco130I CCWWGG 3 cut(s) 431, 451, 823
Eco24I GRGCYC 1 cut(s) 2111
Eco31I GGTCTC 1 cut(s) 650
Eco47I GGWCC 5 cut(s) 33, 156, 605, 619, 1864
Eco53kI GAGCTC 1 cut(s) 2109
Eco57I CTGAAG 5 cut(s) 149, 621, 771, 1565, 1653
Eco88I CYCGRG 3 cut(s) 92, 653, 1472
EcoICRI GAGCTC 1 cut(s) 2109
EcoO109I RGGNCCY 1 cut(s) 33
EcoRI GAATTC 1 cut(s) 1335
EcoRII CCWGG 7 cut(s) 179, 417, 621, 787, 1507, 1690, 1800
EcoT14I CCWWGG 3 cut(s) 431, 451, 823
EcoT38I GRGCYC 1 cut(s) 2111
ErhI CCWWGG 3 cut(s) 431, 451, 823
Esp3I CGTCTC 1 cut(s) 2046
FaeI CATG 5 cut(s) 512, 1355, 1861, 1906, 2071
FaqI GGGAC 3 cut(s) 46, 391, 1693
FatI CATG 5 cut(s) 508, 1351, 1857, 1902, 2067
FauI CCCGC 1 cut(s) 146
FbaI TGATCA 3 cut(s) 1593, 1746, 2131
FblI GTMKAC 3 cut(s) 249, 459, 994
FokI GGATG 3 cut(s) 754, 1043, 1794
FriOI GRGCYC 1 cut(s) 2111
FspBI CTAG 5 cut(s) 452, 960, 1662, 1683, 2123
GlaI GCGC 1 cut(s) 1236
GsuI CTGGAG 3 cut(s) 252, 363, 1271
HaeIII GGCC 3 cut(s) 644, 1060, 1852
HapII CCGG 3 cut(s) 93, 166, 1867
HgaI GACGC 2 cut(s) 1214, 2028
HhaI GCGC 1 cut(s) 1237
Hin1II CATG 5 cut(s) 512, 1355, 1861, 1906, 2071
Hin6I GCGC 1 cut(s) 1235
HinP1I GCGC 1 cut(s) 1235
HincII GTYRAC 2 cut(s) 238, 1012
HindII GTYRAC 2 cut(s) 238, 1012
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 7 cut(s) 55, 118, 427, 629, 991, 1462, 1870
HpaII CCGG 3 cut(s) 93, 166, 1867
HphI GGTGA 4 cut(s) 709, 1204, 1522, 1706
Hpy166II GTNNAC 7 cut(s) 109, 238, 250, 460, 700, 995, 1012
Hpy8I GTNNAC 7 cut(s) 109, 238, 250, 460, 700, 995, 1012
HpyAV CCTTC 8 cut(s) 124, 490, 596, 716, 1350, 1628, 1802, 1836
HpyCH4III ACNGT 7 cut(s) 271, 1024, 1132, 1246, 1362, 1393, 1944
HpyCH4IV ACGT 2 cut(s) 745, 1986
HpyF10VI GCNNNNNNNGC 9 cut(s) 313, 761, 974, 977, 980, 983, 1096, 1222, 1858
HpyF3I CTNAG 9 cut(s) 8, 521, 867, 920, 1266, 1288, 1344, 2105, 2112
HpySE526I ACGT 2 cut(s) 745, 1986
Hsp92II CATG 5 cut(s) 512, 1355, 1861, 1906, 2071
HspAI GCGC 1 cut(s) 1235
Kpn2I TCCGGA 1 cut(s) 1866
Ksp22I TGATCA 3 cut(s) 1593, 1746, 2131
LmnI GCTCC 4 cut(s) 321, 1039, 1744, 2071
Lsp1109I GCAGC 9 cut(s) 928, 943, 958, 967, 986, 995, 1661, 1889, 2011
LweI GCATC 4 cut(s) 1021, 1785, 2011, 2134
MabI ACCWGGT 1 cut(s) 621
MaeI CTAG 5 cut(s) 452, 960, 1662, 1683, 2123
MaeII ACGT 2 cut(s) 745, 1986
MaeIII GTNAC 7 cut(s) 331, 475, 1024, 1414, 1528, 1897, 1982
MfeI CAATTG 1 cut(s) 1713
MflI RGATCY 3 cut(s) 4, 123, 139
MhlI GDGCHC 1 cut(s) 2111
MlsI TGGCCA 1 cut(s) 1852
MluNI TGGCCA 1 cut(s) 1852
MlyI GAGTC 2 cut(s) 436, 1000
Mox20I TGGCCA 1 cut(s) 1852
MroI TCCGGA 1 cut(s) 1866
MroXI GAANNNNTTC 1 cut(s) 1339
MscI TGGCCA 1 cut(s) 1852
MseI TTAA 3 cut(s) 263, 536, 876
MslI CAYNNNNRTG 1 cut(s) 1535
Msp20I TGGCCA 1 cut(s) 1852
MspA1I CMGCKG 1 cut(s) 977
MspCI CTTAAG 1 cut(s) 262
MspI CCGG 3 cut(s) 93, 166, 1867
MunI CAATTG 1 cut(s) 1713
Mva1269I GAATGC 1 cut(s) 1169
MvaI CCWGG 7 cut(s) 181, 419, 623, 789, 1509, 1692, 1802
MvnI CGCG 1 cut(s) 101
MwoI GCNNNNNNNGC 9 cut(s) 313, 761, 974, 977, 980, 983, 1096, 1222, 1858
NciI CCSGG 3 cut(s) 93, 94, 166
NlaIII CATG 5 cut(s) 512, 1355, 1861, 1906, 2071
NlaIV GGNNCC 6 cut(s) 34, 125, 141, 157, 170, 1429
NmeAIII GCCGAG 1 cut(s) 1354
NmuCI GTSAC 3 cut(s) 331, 1024, 1528
NspI RCATGY 2 cut(s) 512, 1861
NspV TTCGAA 2 cut(s) 1795, 2139
OliI CACNNNNGTG 1 cut(s) 1535
PaeR7I CTCGAG 1 cut(s) 1472
PciI ACATGT 1 cut(s) 508
PctI GAATGC 1 cut(s) 1169
PdmI GAANNNNTTC 1 cut(s) 1339
PfeI GAWTC 5 cut(s) 55, 118, 629, 1462, 1870
PflFI GACNNNGTC 1 cut(s) 623
PfoI TCCNGGA 1 cut(s) 787
PleI GAGTC 2 cut(s) 435, 999
PpsI GAGTC 2 cut(s) 435, 999
PpuMI RGGWCCY 1 cut(s) 33
PscI ACATGT 1 cut(s) 508
PshBI ATTAAT 1 cut(s) 876
PsiI TTATAA 2 cut(s) 1280, 2082
Psp124BI GAGCTC 1 cut(s) 2111
Psp5II RGGWCCY 1 cut(s) 33
Psp6I CCWGG 7 cut(s) 179, 417, 621, 787, 1507, 1690, 1800
PspGI CCWGG 7 cut(s) 179, 417, 621, 787, 1507, 1690, 1800
PspN4I GGNNCC 6 cut(s) 34, 125, 141, 157, 170, 1429
PspPI GGNCC 6 cut(s) 33, 156, 605, 619, 642, 1864
PspPPI RGGWCCY 1 cut(s) 33
PstI CTGCAG 2 cut(s) 976, 985
PstNI CAGNNNCTG 2 cut(s) 329, 980
PsuI RGATCY 3 cut(s) 4, 123, 139
PsyI GACNNNGTC 1 cut(s) 623
RsaI GTAC 7 cut(s) 110, 860, 1359, 1441, 1769, 1892, 2121
RsaNI GTAC 7 cut(s) 109, 859, 1358, 1440, 1768, 1891, 2120
RseI CAYNNNNRTG 1 cut(s) 1535
SacI GAGCTC 1 cut(s) 2111
SaqAI TTAA 3 cut(s) 263, 536, 876
Sau96I GGNCC 6 cut(s) 33, 156, 605, 619, 642, 1864
ScaI AGTACT 2 cut(s) 860, 2121
SchI GAGTC 2 cut(s) 436, 1000
SduI GDGCHC 1 cut(s) 2111
SexAI ACCWGGT 1 cut(s) 621
SfaNI GCATC 4 cut(s) 1021, 1785, 2011, 2134
SfcI CTRYAG 6 cut(s) 681, 945, 972, 981, 1419, 1990
Sfr274I CTCGAG 1 cut(s) 1472
SfuI TTCGAA 2 cut(s) 1795, 2139
SinI GGWCC 5 cut(s) 33, 156, 605, 619, 1864
SlaI CTCGAG 1 cut(s) 1472
SmaI CCCGGG 1 cut(s) 94
SmiMI CAYNNNNRTG 1 cut(s) 1535
SmlI CTYRAG 3 cut(s) 262, 1238, 1472
SmoI CTYRAG 3 cut(s) 262, 1238, 1472
SpeI ACTAGT 1 cut(s) 959
SsiI CCGC 5 cut(s) 101, 153, 968, 977, 1096
SspI AATATT 1 cut(s) 1379
SspMI CTAG 5 cut(s) 452, 960, 1662, 1683, 2123
SstI GAGCTC 1 cut(s) 2111
StyI CCWWGG 3 cut(s) 431, 451, 823
TaaI ACNGT 7 cut(s) 271, 1024, 1132, 1246, 1362, 1393, 1944
TaiI ACGT 2 cut(s) 748, 1989
TaqI TCGA 5 cut(s) 627, 1473, 1795, 2008, 2139
TatI WGTACW 5 cut(s) 108, 858, 1439, 1767, 2119
TauI GCSGC 3 cut(s) 971, 980, 1099
TfiI GAWTC 5 cut(s) 55, 118, 629, 1462, 1870
Tru1I TTAA 3 cut(s) 263, 536, 876
Tru9I TTAA 3 cut(s) 263, 536, 876
TscAI CASTG 9 cut(s) 247, 274, 340, 346, 678, 871, 1251, 1396, 1749
TseFI GTSAC 3 cut(s) 331, 1024, 1528
TseI GCWGC 9 cut(s) 931, 941, 971, 974, 980, 983, 1649, 1877, 2024
Tsp45I GTSAC 3 cut(s) 331, 1024, 1528
TspDTI ATGAA 4 cut(s) 621, 894, 1150, 1845
TspGWI ACGGA 2 cut(s) 132, 1043
TspMI CCCGGG 1 cut(s) 92
TspRI CASTG 9 cut(s) 247, 274, 340, 346, 678, 871, 1251, 1396, 1749
Tth111I GACNNNGTC 1 cut(s) 623
Vha464I CTTAAG 1 cut(s) 262
VpaK11BI GGWCC 5 cut(s) 33, 156, 605, 619, 1864
VspI ATTAAT 1 cut(s) 876
XapI RAATTY 4 cut(s) 1335, 1928, 2000, 2076
XceI RCATGY 2 cut(s) 512, 1861
XhoI CTCGAG 1 cut(s) 1472
XmaI CCCGGG 1 cut(s) 92
XmaJI CCTAGG 1 cut(s) 451
XmiI GTMKAC 3 cut(s) 249, 459, 994
XmnI GAANNNNTTC 1 cut(s) 1339
XspI CTAG 5 cut(s) 452, 960, 1662, 1683, 2123
ZrmI AGTACT 2 cut(s) 860, 2121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.