pycom05g26410

phospho-N-acetylmuramoyl-pentapeptide- transferase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008615
Physical Location & Seq
Forward (+)
27712763 .. 27716298
3536 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1494 bp
ATGACGTCACCATCATCTTGTTCTCTCACTCGCCTCCGGCACCTCTCTCCTCTCCAACTCTCTCGCTCCCGCCACTGCTCCCCCTCGCTCTGCACCGCCATTCGCCGCCGATCCGTTTCGGCCTTTTCGTTCGGGAGCTCCGGTGTCCGATACGATCTTCAGTTGATTCGGCGGAGAAATGGGCGCCAATTCCGATACAATTCTGTTGATTTCGGGTCTTTCGATTCGGAAGCAGCTGATATAGCACCACTTGATGACTGGGGGGGCGGCCATGAAGACTCGACAGGGTACATAATGTATTCCAGTGGTGAAGATAGTGATGGAGAGTTCGTAGTAACTCCCGTAGCTGATATTGATTTGCCTGCCATCACTGTATCAACTGATGATGCTATGACTGTCACTGCTCATCGGCTTGCAATGCTTGGAAGGCAGCGTAGGAAACACAGGGTTAGACATGGAGTATTGCTCAATATGGGACTGATACTGTTCTTAGTGGTGTTACTTTTATATGTGGATTCGTGTGCATGGAGGATTGTTAGACTACCTTTGGCCCCATTTCACTTGACCTGCCCTTTTTTTGTATCAGCTGTTTTAGCTTCGTGCGCAGGATATGTTTGTGTTCCCCTACTCTATGTTTTGAAGATTCGTCAAATTATCAGAAAAGAAGGGCCTGCAAGGCACTCCTTAAAGGAAAGAACTCCCACGATGGGTGGATTATTTTTTATACCAATTGGTGTAATTGTTGCGAAATTTATTGCCGGGTTTTCTTCTGTTGAAGTTTCTGGAGCAGCTGCAGCAACTCTTGCATTTGCTGCAATTGGTCTGCTTGATGATATCTTAAGCCTCGTAGCTGTTGGAACCTGGTTTTCGTATTGGTTGCATACCGCAAATATATCATCACCCTATGGCATGAAACTGTTGGTTCCTTTACCTGCACTAGGGCTTGTGTTCCTGGGAAAATGTTATTTAATGTTGGCTTCATTCTGTTTCGTTTCTATGGGAAATGGGGTTAACTTAACAGATGGTCTTGATGGTCTGGCTGGAGGGTCTGCTGCATTGGCTTTTGCAGGAATGTCGATCGCAGTGCTTCCAATATGTTCGGACCTTGCTATATTTGGAGCATCAATGGCAGGAGCTTGTGTCGGTTTTCTTTTGCACAACCGATACAAGGCATCAATATTCATGGGTGATACCGGATCCCTGGCACTTGGTGGAGGGTTAGCTGCAATGGCTTGTTGCACGGGGATGTTCTTTCCCTTATTTATTTCATCTGGAGTCTTTGTCATGGAAGCCTCATCAGTTATTTTGCAGGTATTATACTTCAAGACAACCAAGCGTTTACACGGAGCTGGGCGCCGGCTCTTCCGAATGGCACCGTTCCATCACCACCTTGAACTATGTGGATTAAAAGAACCAGTCATTGTTGCTGGTGCTTACGTTATATCGTCCACACTGGCGTTGTGTGCTGGCTATCTCGGCCTCGTTTCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00550 ko01100 ko01502 map00550 map01100 map01502
Pfam Domains
Protein Families
Glycos_transf_4 MraY_sig1

Protein Analysis

498

Amino Acids

53.24

Weight (kDa)

9.02

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycos_transf_4 PF00953 263 - 412 1.2e-21 Glycosyl transferase family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
Acc16I TGCGCA 1 cut(s) 604
Acc36I ACCTGC 3 cut(s) 575, 940, 1300
AccB1I GGYRCC 4 cut(s) 39, 183, 1353, 1372
AciI CCGC 6 cut(s) 70, 96, 106, 172, 267, 885
AclWI GGATC 3 cut(s) 105, 1191, 1204
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 1 cut(s) 749
AcuI CTGAAG 1 cut(s) 143
AcyI GRCGYC 3 cut(s) 5, 184, 1354
AdeI CACNNNGTG 1 cut(s) 1211
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 3 cut(s) 707, 938, 1168
AflII CTTAAG 1 cut(s) 838
AgsI TTSAA 4 cut(s) 640, 776, 1324, 1394
AjnI CCWGG 3 cut(s) 860, 951, 1200
Alw21I GWGCWC 1 cut(s) 140
AlwI GGATC 3 cut(s) 105, 1191, 1204
AoxI GGCC 5 cut(s) 120, 268, 549, 668, 1477
ApeKI GCWGC 8 cut(s) 233, 430, 788, 791, 794, 812, 1052, 1223
ApoI RAATTY 1 cut(s) 749
AspLEI GCGC 3 cut(s) 186, 605, 1356
AspS9I GGNCC 3 cut(s) 550, 668, 1102
AsuC2I CCSGG 1 cut(s) 760
AsuHPI GGTGA 4 cut(s) 320, 891, 1199, 1376
AvaII GGWCC 1 cut(s) 1102
BamHI GGATCC 1 cut(s) 1196
BanI GGYRCC 4 cut(s) 39, 183, 1353, 1372
BanII GRGCYC 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 140
BbvI GCAGC 8 cut(s) 245, 442, 778, 799, 800, 806, 1039, 1210
BccI CCATC 7 cut(s) 19, 314, 374, 700, 1016, 1025, 1389
BcgI CGANNNNNNTGC 4 cut(s) 1056, 1066, 1090, 1100
BciT130I CCWGG 3 cut(s) 862, 953, 1202
BcnI CCSGG 1 cut(s) 760
BfaI CTAG 1 cut(s) 938
BfmI CTRYAG 1 cut(s) 792
BfoI RGCGCY 2 cut(s) 187, 1357
BfrI CTTAAG 1 cut(s) 838
BfuAI ACCTGC 3 cut(s) 575, 940, 1300
BglI GCCNNNNNGGC 1 cut(s) 676
Bme1390I CCNGG 4 cut(s) 760, 862, 953, 1202
Bme18I GGWCC 1 cut(s) 1102
BmgT120I GGNCC 3 cut(s) 550, 668, 1102
BmiI GGNNCC 8 cut(s) 41, 185, 552, 859, 924, 1198, 1355, 1374
BmrFI CCNGG 4 cut(s) 760, 862, 953, 1202
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 3 cut(s) 376, 1130, 1181
BmuI ACTGGG 1 cut(s) 268
BpiI GAAGAC 1 cut(s) 282
BplI GAGNNNNNCTC 2 cut(s) 450, 482
BpmI CTGGAG 3 cut(s) 804, 1062, 1293
BpuMI CCSGG 1 cut(s) 760
BsaHI GRCGYC 3 cut(s) 5, 184, 1354
BsaJI CCNNGG 2 cut(s) 952, 1200
BsaWI WCCGGW 2 cut(s) 140, 1193
BsaXI ACNNNNNCTCC 8 cut(s) 127, 157, 315, 345, 1125, 1155, 1266, 1296
Bsc4I CCNNNNNNNGG 3 cut(s) 707, 938, 1168
Bse118I RCCGGY 1 cut(s) 1356
Bse1I ACTGG 4 cut(s) 263, 303, 1415, 1458
Bse3DI GCAATG 2 cut(s) 423, 1233
BseBI CCWGG 3 cut(s) 862, 953, 1202
BseDI CCNNGG 2 cut(s) 952, 1200
BseGI GGATG 1 cut(s) 1251
BseLI CCNNNNNNNGG 3 cut(s) 707, 938, 1168
BseMI GCAATG 2 cut(s) 423, 1233
BseNI ACTGG 4 cut(s) 263, 303, 1415, 1458
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 8 cut(s) 245, 442, 778, 799, 800, 806, 1039, 1210
BseYI CCCAGC 1 cut(s) 1349
BsgI GTGCAG 2 cut(s) 76, 918
Bsh1285I CGRYCG 1 cut(s) 1080
BshFI GGCC 5 cut(s) 122, 270, 551, 670, 1479
BshNI GGYRCC 4 cut(s) 39, 183, 1353, 1372
BsiEI CGRYCG 1 cut(s) 1080
BsiHKAI GWGCWC 1 cut(s) 140
BsiSI CCGG 5 cut(s) 37, 141, 759, 1194, 1357
BslFI GGGAC 1 cut(s) 489
BslI CCNNNNNNNGG 3 cut(s) 707, 938, 1168
BsmFI GGGAC 1 cut(s) 489
BsnI GGCC 5 cut(s) 122, 270, 551, 670, 1479
Bsp1286I GDGCHC 1 cut(s) 140
Bsp143I GATC 4 cut(s) 110, 154, 1077, 1196
BspACI CCGC 6 cut(s) 70, 96, 106, 172, 267, 885
BspANI GGCC 5 cut(s) 122, 270, 551, 670, 1479
BspLI GGNNCC 8 cut(s) 41, 185, 552, 859, 924, 1198, 1355, 1374
BspMAI CTGCAG 1 cut(s) 796
BspMI ACCTGC 3 cut(s) 575, 940, 1300
BspPI GGATC 3 cut(s) 105, 1191, 1204
BspQI GCTCTTC 1 cut(s) 1367
BspT107I GGYRCC 4 cut(s) 39, 183, 1353, 1372
BspTI CTTAAG 1 cut(s) 838
BsrDI GCAATG 2 cut(s) 423, 1233
BsrFI RCCGGY 1 cut(s) 1356
BsrI ACTGG 4 cut(s) 263, 303, 1415, 1458
BssAI RCCGGY 1 cut(s) 1356
BssECI CCNNGG 2 cut(s) 952, 1200
BssMI GATC 4 cut(s) 110, 154, 1077, 1196
BssNI GRCGYC 3 cut(s) 5, 184, 1354
Bst2UI CCWGG 3 cut(s) 862, 953, 1202
Bst4CI ACNGT 5 cut(s) 373, 397, 486, 918, 1377
Bst6I CTCTTC 1 cut(s) 1367
BstACI GRCGYC 3 cut(s) 5, 184, 1354
BstAFI CTTAAG 1 cut(s) 838
BstAPI GCANNNNNTGC 2 cut(s) 803, 812
BstC8I GCNNGC 5 cut(s) 363, 414, 672, 1358, 1468
BstDEI CTNAG 1 cut(s) 490
BstENI CCTNNNNNAGG 1 cut(s) 936
BstF5I GGATG 1 cut(s) 1251
BstH2I RGCGCY 2 cut(s) 187, 1357
BstHHI GCGC 3 cut(s) 186, 605, 1356
BstKTI GATC 4 cut(s) 113, 157, 1080, 1199
BstMBI GATC 4 cut(s) 110, 154, 1077, 1196
BstMCI CGRYCG 1 cut(s) 1080
BstNI CCWGG 3 cut(s) 862, 953, 1202
BstSCI CCNGG 4 cut(s) 758, 860, 951, 1200
BstSFI CTRYAG 1 cut(s) 792
BstV1I GCAGC 8 cut(s) 245, 442, 778, 799, 800, 806, 1039, 1210
BstV2I GAAGAC 1 cut(s) 282
BstX2I RGATCY 1 cut(s) 1196
BstYI RGATCY 1 cut(s) 1196
BsuRI GGCC 5 cut(s) 122, 270, 551, 670, 1479
BtsCI GGATG 1 cut(s) 1251
BtsI GCAGTG 3 cut(s) 73, 399, 1089
BtsIMutI CAGTG 6 cut(s) 73, 310, 369, 399, 1089, 1451
BveI ACCTGC 3 cut(s) 575, 940, 1300
Cac8I GCNNGC 5 cut(s) 363, 414, 672, 1358, 1468
CfoI GCGC 3 cut(s) 186, 605, 1356
Cfr10I RCCGGY 1 cut(s) 1356
Cfr13I GGNCC 3 cut(s) 550, 668, 1102
CsiI ACCWGGT 1 cut(s) 860
Csp6I GTAC 1 cut(s) 289
CviAII CATG 6 cut(s) 272, 455, 525, 910, 1183, 1285
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 1 cut(s) 490
DinI GGCGCC 2 cut(s) 185, 1355
DpnI GATC 4 cut(s) 112, 156, 1079, 1198
DpnII GATC 4 cut(s) 110, 154, 1077, 1196
DraIII CACNNNGTG 1 cut(s) 1211
EaeI YGGCCR 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 1367
EarI CTCTTC 1 cut(s) 1367
EciI GGCGGA 1 cut(s) 187
Ecl136II GAGCTC 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 140
Eco32I GATATC 1 cut(s) 835
Eco47I GGWCC 1 cut(s) 1102
Eco53kI GAGCTC 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 138
EcoNI CCTNNNNNAGG 1 cut(s) 936
EcoO109I RGGNCCY 1 cut(s) 668
EcoRII CCWGG 3 cut(s) 860, 951, 1200
EcoRV GATATC 1 cut(s) 835
EcoT38I GRGCYC 1 cut(s) 140
EgeI GGCGCC 2 cut(s) 185, 1355
EheI GGCGCC 2 cut(s) 185, 1355
FaeI CATG 6 cut(s) 275, 458, 528, 913, 1186, 1288
FaqI GGGAC 1 cut(s) 489
FatI CATG 6 cut(s) 271, 454, 524, 909, 1182, 1284
FauI CCCGC 1 cut(s) 77
FokI GGATG 1 cut(s) 1258
FriOI GRGCYC 1 cut(s) 140
FspBI CTAG 1 cut(s) 938
FspI TGCGCA 1 cut(s) 604
GlaI GCGC 3 cut(s) 185, 604, 1355
GsaI CCCAGC 1 cut(s) 1353
GsuI CTGGAG 3 cut(s) 804, 1062, 1293
HaeII RGCGCY 2 cut(s) 187, 1357
HaeIII GGCC 5 cut(s) 122, 270, 551, 670, 1479
HapII CCGG 5 cut(s) 37, 141, 759, 1194, 1357
HhaI GCGC 3 cut(s) 186, 605, 1356
Hin1I GRCGYC 3 cut(s) 5, 184, 1354
Hin1II CATG 6 cut(s) 275, 458, 528, 913, 1186, 1288
Hin6I GCGC 3 cut(s) 184, 603, 1354
HinP1I GCGC 3 cut(s) 184, 603, 1354
HincII GTYRAC 1 cut(s) 1012
HindII GTYRAC 1 cut(s) 1012
HinfI GANTC 6 cut(s) 166, 224, 278, 515, 643, 1275
HpaI GTTAAC 1 cut(s) 1012
HpaII CCGG 5 cut(s) 37, 141, 759, 1194, 1357
HphI GGTGA 4 cut(s) 320, 891, 1199, 1376
Hpy166II GTNNAC 3 cut(s) 1012, 1340, 1449
Hpy188I TCNGA 6 cut(s) 149, 194, 229, 659, 1102, 1367
Hpy188III TCNNGA 5 cut(s) 133, 783, 1028, 1272, 1324
Hpy8I GTNNAC 3 cut(s) 1012, 1340, 1449
HpyAV CCTTC 2 cut(s) 420, 659
HpyCH4III ACNGT 5 cut(s) 373, 397, 486, 918, 1377
HpyCH4IV ACGT 2 cut(s) 5, 1437
HpyF3I CTNAG 1 cut(s) 490
HpySE526I ACGT 2 cut(s) 5, 1437
Hsp92I GRCGYC 3 cut(s) 5, 184, 1354
Hsp92II CATG 6 cut(s) 275, 458, 528, 913, 1186, 1288
HspAI GCGC 3 cut(s) 184, 603, 1354
KasI GGCGCC 2 cut(s) 183, 1353
KroI GCCGGC 1 cut(s) 1356
KroNI GCCGGC 1 cut(s) 1358
KspAI GTTAAC 1 cut(s) 1012
Kzo9I GATC 4 cut(s) 110, 154, 1077, 1196
LguI GCTCTTC 1 cut(s) 1367
LmnI GCTCC 8 cut(s) 71, 83, 135, 143, 785, 1118, 1133, 1346
Lsp1109I GCAGC 8 cut(s) 245, 442, 778, 799, 800, 806, 1039, 1210
LweI GCATC 3 cut(s) 376, 1130, 1181
MabI ACCWGGT 1 cut(s) 860
MaeI CTAG 1 cut(s) 938
MaeII ACGT 2 cut(s) 5, 1437
MaeIII GTNAC 4 cut(s) 6, 334, 397, 498
MalI GATC 4 cut(s) 112, 156, 1079, 1198
MboI GATC 4 cut(s) 110, 154, 1077, 1196
MboII GAAGA 6 cut(s) 149, 287, 323, 652, 759, 1354
MfeI CAATTG 2 cut(s) 729, 816
MflI RGATCY 1 cut(s) 1196
MhlI GDGCHC 1 cut(s) 140
MluCI AATT 7 cut(s) 188, 199, 651, 729, 738, 749, 816
Mly113I GGCGCC 2 cut(s) 184, 1354
MlyI GAGTC 2 cut(s) 272, 1284
MmeI TCCRAC 2 cut(s) 79, 835
MroNI GCCGGC 1 cut(s) 1356
MseI TTAA 6 cut(s) 686, 839, 968, 1011, 1016, 1406
MslI CAYNNNNRTG 1 cut(s) 1244
MspA1I CMGCKG 3 cut(s) 236, 587, 791
MspCI CTTAAG 1 cut(s) 838
MspI CCGG 5 cut(s) 37, 141, 759, 1194, 1357
MspR9I CCNGG 4 cut(s) 760, 862, 953, 1202
MunI CAATTG 2 cut(s) 729, 816
MvaI CCWGG 3 cut(s) 862, 953, 1202
NaeI GCCGGC 1 cut(s) 1358
NarI GGCGCC 2 cut(s) 184, 1354
NciI CCSGG 1 cut(s) 760
NdeII GATC 4 cut(s) 110, 154, 1077, 1196
NgoMIV GCCGGC 1 cut(s) 1356
NlaIII CATG 6 cut(s) 275, 458, 528, 913, 1186, 1288
NlaIV GGNNCC 8 cut(s) 41, 185, 552, 859, 924, 1198, 1355, 1374
NmeAIII GCCGAG 1 cut(s) 1455
NmuCI GTSAC 2 cut(s) 6, 397
NsbI TGCGCA 1 cut(s) 604
PciSI GCTCTTC 1 cut(s) 1367
PcsI WCGNNNNNNNCGW 2 cut(s) 125, 219
PdiI GCCGGC 1 cut(s) 1358
PfeI GAWTC 4 cut(s) 166, 224, 515, 643
Ple19I CGATCG 1 cut(s) 1080
PleI GAGTC 2 cut(s) 272, 1283
PluTI GGCGCC 2 cut(s) 187, 1357
PpsI GAGTC 2 cut(s) 272, 1283
Psp124BI GAGCTC 1 cut(s) 140
Psp6I CCWGG 3 cut(s) 860, 951, 1200
PspFI CCCAGC 1 cut(s) 1349
PspGI CCWGG 3 cut(s) 860, 951, 1200
PspN4I GGNNCC 8 cut(s) 41, 185, 552, 859, 924, 1198, 1355, 1374
PspPI GGNCC 3 cut(s) 550, 668, 1102
PstI CTGCAG 1 cut(s) 796
PsuI RGATCY 1 cut(s) 1196
PvuI CGATCG 1 cut(s) 1080
PvuII CAGCTG 3 cut(s) 236, 587, 791
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
RseI CAYNNNNRTG 1 cut(s) 1244
SacI GAGCTC 1 cut(s) 140
SapI GCTCTTC 1 cut(s) 1367
SaqAI TTAA 6 cut(s) 686, 839, 968, 1011, 1016, 1406
Sau3AI GATC 4 cut(s) 110, 154, 1077, 1196
Sau96I GGNCC 3 cut(s) 550, 668, 1102
SchI GAGTC 2 cut(s) 272, 1284
ScrFI CCNGG 4 cut(s) 760, 862, 953, 1202
SduI GDGCHC 1 cut(s) 140
SexAI ACCWGGT 1 cut(s) 860
SfaNI GCATC 3 cut(s) 376, 1130, 1181
SfcI CTRYAG 1 cut(s) 792
SfoI GGCGCC 2 cut(s) 185, 1355
SinI GGWCC 1 cut(s) 1102
SmiMI CAYNNNNRTG 1 cut(s) 1244
SmlI CTYRAG 1 cut(s) 838
SmoI CTYRAG 1 cut(s) 838
Sse9I AATT 7 cut(s) 188, 199, 651, 729, 738, 749, 816
SsiI CCGC 6 cut(s) 70, 96, 106, 172, 267, 885
SspDI GGCGCC 2 cut(s) 183, 1353
SspI AATATT 1 cut(s) 1179
SspMI CTAG 1 cut(s) 938
SstI GAGCTC 1 cut(s) 140
StyD4I CCNGG 4 cut(s) 758, 860, 951, 1200
TaaI ACNGT 5 cut(s) 373, 397, 486, 918, 1377
TaiI ACGT 2 cut(s) 8, 1440
TaqI TCGA 3 cut(s) 222, 281, 1076
TasI AATT 7 cut(s) 188, 199, 651, 729, 738, 749, 816
TauI GCSGC 2 cut(s) 108, 270
TfiI GAWTC 4 cut(s) 166, 224, 515, 643
Tru1I TTAA 6 cut(s) 686, 839, 968, 1011, 1016, 1406
Tru9I TTAA 6 cut(s) 686, 839, 968, 1011, 1016, 1406
TscAI CASTG 6 cut(s) 80, 310, 376, 406, 1089, 1458
TseFI GTSAC 2 cut(s) 6, 397
TseI GCWGC 8 cut(s) 233, 430, 788, 791, 794, 812, 1052, 1223
Tsp45I GTSAC 2 cut(s) 6, 397
TspDTI ATGAA 5 cut(s) 288, 926, 969, 1171, 1257
TspGWI ACGGA 2 cut(s) 103, 1359
TspRI CASTG 6 cut(s) 80, 310, 376, 406, 1089, 1458
Vha464I CTTAAG 1 cut(s) 838
VpaK11BI GGWCC 1 cut(s) 1102
XagI CCTNNNNNAGG 1 cut(s) 936
XapI RAATTY 1 cut(s) 749
XcmI CCANNNNNNNNNTGG 1 cut(s) 255
XspI CTAG 1 cut(s) 938
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.