Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0005890

Physical Location & Seq

Genomic Coordinates Reverse (-)

28336252 .. 28339513

Gene Structure 3262 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2442 bp

ATGACTAGGGCTGTTCGCAATAGGATACTCAAGGATGCGAATGGTGATACTAGTGATCATCTGCGCAACCACATCCATTTGACAAATTGCATTCACTTGAAGAACCATATGCACAAGCAAAGCCCCATATTGGCTGACAGGTCACTTATGAGGGACCTTGTTGTCCTTCAGAGGTCGCGATCTCTCAGGGACCCTTCTGCCAGTCCGCCCTCATGGCATTCGCCTTCCATTCTTGATATGCTTTCCAGAAGAGGTGAAAATGATGCCTTGGTGCGTGAGGGGAGAAGGTCAGTAGGAAGTGAGTATCGAAGGGATGCCAGGAGGCTATCAACAGGTTCTCCACCTTTGGCAAGTTTAGCGACACCAAAAGTTGCTCAACGTGGGGCTAATGGGGGAAATGATGGTGTGGCCGGAACCAGTGAACATGGAAGCAAGAGTGGAGTTAGGGATGGTAGGAAAATTAGGAGAGAAGAATCTAGTCAGAAGAGTAAAAGGAGTGAAAGTTTGGGTGGCAATGAGGAGCCTCCGCCAGACCAGAATGGTAACGATTTGACTCATGATGCTCTTTCTGGGAATTCAGAATCCAAAAGTAGAAAAAGTAAACAAAAGGGAAAGCACACCCAAGGTGCTCGAATGAAGACTCTCTCAGAGCAATTGAATGATGGTAGGATGGATACTGATGATGTAGCGTCCTCTAATATCCACCTGCCTGGAAGACGGTCCCGGCAGGGAAGAATCTCTGAGGAACCGGAGGTCAGCGCTCGGGGCTATTGCAGTGGACTAAGTAGAGTAAAAAGGCGGAGATTACGAGGTGCAAGAAGAAGCCGTGCTTCTGTAGCGTCCAGAGACTTTGGACCTCAGCATGGTTTATCTGTAGCTTCTAATTCTATAGCTCAAGGGTCAGCTCACCGAAAGTATCACATGGAACAGGGAGCGGATGAGTATGGTGAGCAAAATGTCACAAGGGCTCCTAGAAACGGGTGTGGGATTCCCTGGAATTGGTCGAGAATCCATCATAGAGGTAAAACATTCCTTGACATTGCTGGAAGGAGCATTTCTTGTGGTTTATCTGATTCAAGGTTTAAGAAGGGTGGTCTGGCTGCCCATGGTAGAGATATTTCTGACATGCCCATGGCAACTGATAACTCGAGCGTTTCTACTAAATCTGAATCTGAGGCACTTCCCTTACTAGTTGAAGCATCTGGATCAAAGGTAAGCTCTGACAATGCTGGTTGGGTACATGACTATTCTGGTGAACTTGGTATTTATGCCGATAATTTATTCAAGCATGACGTCGGTTCCGACTATGCTTCTGAAGCTAGATCTGGTGATCAACGCAAATTGAGGGGACACCGCCGTTGTAGGCACCAAAATCTGACGCAAAAATACATGCCGAGAACCTTTAGAGATCTTGTGGGGCAGAATTTGGTGGCACAAGCTCTTTCAAATGCTGTCATGAAAAAGAAAGTTGGGTTGCTATACGTGTTCTATGGTCCTCATGGCACAGGGAAAACATCATGTGCTCGCATATTTTCTAGAGCTCTAAATTGCCAGTCTGTGGATCATCCCAAGCCTTGTGGCTTTTGCAATTCTTGCATCGCACATGATATGGGCAAGAGTAGAAATATAAAGGAAGTTGGGCCTGTTAGTAACTTCGACTTCGATAGCATTATGGATCTACTTGACAATATGAGTATTTCCCAACTGCCATCACAGTACAGAGTGTTTATCTTTGATGATTGTGATACATTGTCCCCTGAGTGCTGCAGTGCCTTATCGAAGGTCATTGATCGAGCACCCAGACATGTGGTTTTTGTCCTTGTCTGTTCAAGTCTTGATGTTTTGCCTCACATAATCATATCCAGGTGTCAAAAGTTCTTTTTCCCTAAGCTAAGGGATGCAGATATTATTTACAGTTTGCAATGGATAGCAGCCAAAGAAGATCTAGAAATTGACAATGATGCACTGAAACTTATTTCGTCAACGTCAGATGGTTCATTGAGGGATGCTGAGATGACTCTAGAGCAACTGAGTTTACTTGGCCAGAGAATCTCAGTTCCTCTTGTTCAGGAACTGGTTGGGCTTATATCTGATGAGAAATTGATAGATCTTCTTGATTTATCATTATCTGCAGACACAGTTAATACTGTGAAGAATTTGAGAATGATAATGGAAAGTGGAGTTGAGCCATTAGCTTTGATGTCACAACTTGCAACGGTGATTACAGATATACTTGCTGGCAGCTATGATTATAGAAAAGAAAGGCGTAGAAGGAAGTTCTTTCGGAACCAACCATTATCAAAAGAAGACATGGAGAAGTTGCGTCAAGCTTTGAAAACTTTATCTGAGGCCGAGAAACAATTGAGGATGTCAAATGACAAGTTAACATGGCTAACCGCTGCACTTCTCCAATTTGCTCGATCAACAGTATATGTTGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005663 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0071944 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

814

Amino Acids

90.28

Weight (kDa)

9.5

Isoelectric Point (pI)

53.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol3_delta2	PF13177	474 - 631	1.5e-23	DNA polymerase III, delta subunit
AAA	PF00004	495 - 628	3.6e-06	ATPase family associated with various cellular activities (AAA)
DNAX_ATPase_lid	PF22608	638 - 684	9.1e-06	DNA polymerase III clamp loader subunit, ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005890)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18820
fragaria_vesca	FvH4_3g07230 FvH4_3g07230 FvH4_3g07230 FvH4_3g07230 FvH4_3g07230 FvH4_3g07230 FvH4_3g07230 FvH4_3g07230
malus_domestica	MD00G1008200.v1.1 MD10G1274700.v1.1
prunus_persica	Prupe.4G067700_v2.0.a1 Prupe.4G067700_v2.0.a1 Prupe.4G067700_v2.0.a1 Prupe.4G067700_v2.0.a1
pyrus_communis	pycom05g27350 pycom10g22920
rosa_chinensis	RchiOBHm_Chr5g0011921
rosa_laevigata	RLG00000031869
rosa_multiflora	Rmu_sc0001475.1_g000004
rosa_roxburghii	Rroxscaffold_1G00064670
rosa_rugosa	Rorug04G0460000 Rorug04G0460100
rosa_samantha	Rh5AG091500 Rh5BG087300 Rh5CG101200 Rh5DG087900
rosa_wichuraiana	Rw5G007980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	714
AasI	GACNNNNNNGTC	1 cut(s)	161
AatII	GACGTC	1 cut(s)	1296
Acc16I	TGCGCA	1 cut(s)	65
Acc36I	ACCTGC	1 cut(s)	714
AccB1I	GGYRCC	1 cut(s)	1365
AccB7I	CCANNNNNTGG	1 cut(s)	1558
AccBSI	CCGCTC	1 cut(s)	935
AccII	CGCG	1 cut(s)	178
AciI	CCGC	6 cut(s)	206, 527, 799, 935, 1354, 2397
AclWI	GGATC	3 cut(s)	1213, 1569, 1683
AcoI	YGGCCR	2 cut(s)	408, 2041
AcsI	RAATTY	3 cut(s)	574, 1423, 2155
AcuI	CTGAAG	2 cut(s)	152, 1335
AcyI	GRCGYC	1 cut(s)	1293
AfaI	GTAC	2 cut(s)	1239, 1718
AfeI	AGCGCT	1 cut(s)	760
AfiI	CCNNNNNNNGG	5 cut(s)	130, 863, 977, 999, 1558
AflIII	ACRYGT	2 cut(s)	1482, 1804
AgsI	TTSAA	8 cut(s)	100, 658, 1077, 1196, 1285, 1446, 1830, 2335
AhlI	ACTAGT	2 cut(s)	50, 1189
AjnI	CCWGG	4 cut(s)	317, 709, 992, 1862
Alw21I	GWGCWC	4 cut(s)	631, 1525, 1543, 1798
Alw26I	GTCTC	1 cut(s)	840
AlwI	GGATC	3 cut(s)	1213, 1569, 1683
AlwNI	CAGNNNCTG	1 cut(s)	2074
Ama87I	CYCGRG	2 cut(s)	762, 1147
Aor51HI	AGCGCT	1 cut(s)	760
AoxI	GGCC	4 cut(s)	408, 1640, 2041, 2349
ApeKI	GCWGC	5 cut(s)	1100, 1764, 1931, 2241, 2399
ApoI	RAATTY	3 cut(s)	574, 1423, 2155
AspLEI	GCGC	2 cut(s)	66, 761
AspS9I	GGNCC	6 cut(s)	154, 190, 720, 854, 1493, 1640
AsuC2I	CCSGG	1 cut(s)	724
AsuHPI	GGTGA	7 cut(s)	56, 266, 899, 959, 1265, 1340, 2230
AvaI	CYCGRG	2 cut(s)	762, 1147
AvaII	GGWCC	5 cut(s)	154, 190, 720, 854, 1493
BalI	TGGCCA	1 cut(s)	2043
BanI	GGYRCC	1 cut(s)	1365
BanII	GRGCYC	2 cut(s)	970, 1543
BbsI	GAAGAC	3 cut(s)	644, 721, 2313
Bbv12I	GWGCWC	4 cut(s)	631, 1525, 1543, 1798
BbvCI	CCTCAGC	1 cut(s)	858
BbvI	GCAGC	5 cut(s)	1087, 1751, 1943, 2253, 2386
BccI	CCATC	7 cut(s)	395, 443, 656, 664, 1020, 1717, 1985
BceAI	ACGGC	2 cut(s)	810, 1341
BciT130I	CCWGG	4 cut(s)	319, 711, 994, 1864
BciVI	GTATCC	2 cut(s)	18, 667
BclI	TGATCA	2 cut(s)	55, 1330
BcnI	CCSGG	1 cut(s)	724
BcoDI	GTCTC	1 cut(s)	840
BcuI	ACTAGT	2 cut(s)	50, 1189
BfmI	CTRYAG	5 cut(s)	834, 873, 888, 1765, 2130
BfoI	RGCGCY	1 cut(s)	762
BfuAI	ACCTGC	1 cut(s)	714
BfuI	GTATCC	2 cut(s)	18, 667
BglI	GCCNNNNNGGC	1 cut(s)	214
BglII	AGATCT	4 cut(s)	1322, 1408, 1942, 2107
BisI	GCNGC	5 cut(s)	1101, 1765, 1932, 2242, 2400
BlsI	GCNGC	5 cut(s)	1102, 1766, 1933, 2243, 2401
Bme1390I	CCNGG	5 cut(s)	319, 711, 724, 994, 1864
Bme18I	GGWCC	5 cut(s)	154, 190, 720, 854, 1493
BmeT110I	CYCGRG	2 cut(s)	762, 1147
BmgT120I	GGNCC	6 cut(s)	154, 190, 720, 854, 1493, 1640
BmrFI	CCNGG	5 cut(s)	319, 711, 724, 994, 1864
BmsI	GCATC	9 cut(s)	25, 253, 304, 550, 1208, 1605, 1888, 1951, 1996
BpiI	GAAGAC	3 cut(s)	644, 721, 2313
Bpu10I	CCTNAGC	3 cut(s)	858, 1887, 1892
BpuEI	CTTGAG	2 cut(s)	14, 879
BpuMI	CCSGG	1 cut(s)	724
BsaAI	YACGTR	1 cut(s)	1483
BsaHI	GRCGYC	1 cut(s)	1293
BsaJI	CCNNGG	5 cut(s)	267, 622, 992, 1105, 1131
BsaWI	WCCGGW	1 cut(s)	748
BsaXI	ACNNNNNCTCC	6 cut(s)	322, 352, 487, 517, 952, 982
Bsc4I	CCNNNNNNNGG	5 cut(s)	130, 863, 977, 999, 1558
Bse1I	ACTGG	4 cut(s)	201, 417, 1552, 2079
Bse3DI	GCAATG	3 cut(s)	520, 1038, 1928
BseBI	CCWGG	4 cut(s)	319, 711, 994, 1864
BseDI	CCNNGG	5 cut(s)	267, 622, 992, 1105, 1131
BseLI	CCNNNNNNNGG	5 cut(s)	130, 863, 977, 999, 1558
BseMI	GCAATG	3 cut(s)	520, 1038, 1928
BseNI	ACTGG	4 cut(s)	201, 417, 1552, 2079
BseRI	GAGGAG	1 cut(s)	533
BseXI	GCAGC	5 cut(s)	1087, 1751, 1943, 2253, 2386
BsgI	GTGCAG	1 cut(s)	2385
Bsh1236I	CGCG	1 cut(s)	178
BshFI	GGCC	4 cut(s)	410, 1642, 2043, 2351
BshNI	GGYRCC	1 cut(s)	1365
BsiHKAI	GWGCWC	4 cut(s)	631, 1525, 1543, 1798
BsiHKCI	CYCGRG	2 cut(s)	762, 1147
BsiSI	CCGG	3 cut(s)	411, 724, 749
BslFI	GGGAC	5 cut(s)	167, 203, 706, 1362, 1738
BslI	CCNNNNNNNGG	5 cut(s)	130, 863, 977, 999, 1558
BsmAI	GTCTC	1 cut(s)	840
BsmFI	GGGAC	5 cut(s)	167, 203, 706, 1362, 1738
BsmI	GAATGC	2 cut(s)	90, 217
BsnI	GGCC	4 cut(s)	410, 1642, 2043, 2351
BsoBI	CYCGRG	2 cut(s)	762, 1147
Bsp1286I	GDGCHC	5 cut(s)	631, 970, 1525, 1543, 1798
Bsp19I	CCATGG	2 cut(s)	1105, 1131
Bsp68I	TCGCGA	1 cut(s)	178
BspACI	CCGC	6 cut(s)	206, 527, 799, 935, 1354, 2397
BspANI	GGCC	4 cut(s)	410, 1642, 2043, 2351
BspFNI	CGCG	1 cut(s)	178
BspHI	TCATGA	2 cut(s)	556, 1455
BspMAI	CTGCAG	2 cut(s)	1769, 2134
BspMI	ACCTGC	1 cut(s)	714
BspPI	GGATC	3 cut(s)	1213, 1569, 1683
BspT107I	GGYRCC	1 cut(s)	1365
BsrBI	CCGCTC	1 cut(s)	935
BsrDI	GCAATG	3 cut(s)	520, 1038, 1928
BsrI	ACTGG	4 cut(s)	201, 417, 1552, 2079
BssECI	CCNNGG	5 cut(s)	267, 622, 992, 1105, 1131
BssNI	GRCGYC	1 cut(s)	1293
BssT1I	CCWWGG	4 cut(s)	267, 622, 1105, 1131
Bst2UI	CCWGG	4 cut(s)	319, 711, 994, 1864
Bst4CI	ACNGT	7 cut(s)	720, 1716, 1916, 2140, 2149, 2218, 2428
Bst6I	CTCTTC	2 cut(s)	244, 479
BstACI	GRCGYC	1 cut(s)	1293
BstAPI	GCANNNNNTGC	1 cut(s)	1593
BstBAI	YACGTR	1 cut(s)	1483
BstC8I	GCNNGC	2 cut(s)	1525, 2239
BstDSI	CCRYGG	2 cut(s)	1105, 1131
BstFNI	CGCG	1 cut(s)	178
BstH2I	RGCGCY	1 cut(s)	762
BstHHI	GCGC	2 cut(s)	66, 761
BstMAI	GTCTC	1 cut(s)	840
BstMWI	GCNNNNNNNGC	6 cut(s)	214, 356, 765, 836, 1316, 1593
BstNI	CCWGG	4 cut(s)	319, 711, 994, 1864
BstNSI	RCATGY	3 cut(s)	1129, 1393, 1808
BstSCI	CCNGG	5 cut(s)	317, 709, 722, 992, 1862
BstSFI	CTRYAG	5 cut(s)	834, 873, 888, 1765, 2130
BstUI	CGCG	1 cut(s)	178
BstV1I	GCAGC	5 cut(s)	1087, 1751, 1943, 2253, 2386
BstV2I	GAAGAC	3 cut(s)	644, 721, 2313
BstX2I	RGATCY	5 cut(s)	1322, 1408, 1675, 1942, 2107
BstXI	CCANNNNNNTGG	2 cut(s)	710, 1807
BstYI	RGATCY	5 cut(s)	1322, 1408, 1675, 1942, 2107
BsuI	GTATCC	2 cut(s)	18, 667
BsuRI	GGCC	4 cut(s)	410, 1642, 2043, 2351
BtgI	CCRYGG	2 cut(s)	1105, 1131
BtgZI	GCGATG	1 cut(s)	1582
BtsI	GCAGTG	2 cut(s)	781, 1774
BtsIMutI	CAGTG	4 cut(s)	424, 781, 1774, 1964
BtuMI	TCGCGA	1 cut(s)	178
BveI	ACCTGC	1 cut(s)	714
Cac8I	GCNNGC	2 cut(s)	1525, 2239
CaiI	CAGNNNCTG	1 cut(s)	2074
CciI	TCATGA	2 cut(s)	556, 1455
CfoI	GCGC	2 cut(s)	66, 761
Cfr13I	GGNCC	6 cut(s)	154, 190, 720, 854, 1493, 1640
CseI	GACGC	4 cut(s)	678, 828, 1387, 2312
Csp6I	GTAC	2 cut(s)	1238, 1717
CspCI	CAANNNNNGTGG	2 cut(s)	1558, 1593
CviQI	GTAC	2 cut(s)	1238, 1717
DrdI	GACNNNNNNGTC	1 cut(s)	161
DseDI	GACNNNNNNGTC	1 cut(s)	161
EaeI	YGGCCR	2 cut(s)	408, 2041
Eam1104I	CTCTTC	2 cut(s)	244, 479
EarI	CTCTTC	2 cut(s)	244, 479
EciI	GGCGGA	3 cut(s)	195, 516, 814
Ecl136II	GAGCTC	1 cut(s)	1541
Eco130I	CCWWGG	4 cut(s)	267, 622, 1105, 1131
Eco24I	GRGCYC	2 cut(s)	970, 1543
Eco47I	GGWCC	5 cut(s)	154, 190, 720, 854, 1493
Eco47III	AGCGCT	1 cut(s)	760
Eco53kI	GAGCTC	1 cut(s)	1541
Eco57I	CTGAAG	2 cut(s)	152, 1335
Eco88I	CYCGRG	2 cut(s)	762, 1147
EcoICRI	GAGCTC	1 cut(s)	1541
EcoO109I	RGGNCCY	2 cut(s)	154, 190
EcoRI	GAATTC	1 cut(s)	574
EcoRII	CCWGG	4 cut(s)	317, 709, 992, 1862
EcoT14I	CCWWGG	4 cut(s)	267, 622, 1105, 1131
EcoT38I	GRGCYC	2 cut(s)	970, 1543
ErhI	CCWWGG	4 cut(s)	267, 622, 1105, 1131
FalI	AAGNNNNNCTT	2 cut(s)	814, 846
FaqI	GGGAC	5 cut(s)	167, 203, 706, 1362, 1738
FauNDI	CATATG	1 cut(s)	108
FbaI	TGATCA	2 cut(s)	55, 1330
Fnu4HI	GCNGC	5 cut(s)	1101, 1765, 1932, 2242, 2400
FriOI	GRGCYC	2 cut(s)	970, 1543
Fsp4HI	GCNGC	5 cut(s)	1101, 1765, 1932, 2242, 2400
FspI	TGCGCA	1 cut(s)	65
GlaI	GCGC	2 cut(s)	65, 760
GluI	GCNGC	5 cut(s)	1101, 1765, 1932, 2242, 2400
HaeII	RGCGCY	1 cut(s)	762
HaeIII	GGCC	4 cut(s)	410, 1642, 2043, 2351
HapII	CCGG	3 cut(s)	411, 724, 749
HgaI	GACGC	4 cut(s)	678, 828, 1387, 2312
HhaI	GCGC	2 cut(s)	66, 761
Hin1I	GRCGYC	1 cut(s)	1293
Hin6I	GCGC	2 cut(s)	64, 759
HinP1I	GCGC	2 cut(s)	64, 759
HincII	GTYRAC	2 cut(s)	1983, 2385
HindII	GTYRAC	2 cut(s)	1983, 2385
HindIII	AAGCTT	1 cut(s)	2328
HpaI	GTTAAC	1 cut(s)	2385
HpaII	CCGG	3 cut(s)	411, 724, 749
HphI	GGTGA	7 cut(s)	56, 266, 899, 959, 1265, 1340, 2230
Hpy166II	GTNNAC	7 cut(s)	422, 602, 779, 1256, 1983, 2036, 2385
Hpy8I	GTNNAC	7 cut(s)	422, 602, 779, 1256, 1983, 2036, 2385
Hpy99I	CGWCG	1 cut(s)	1298
HpyAV	CCTTC	9 cut(s)	176, 204, 234, 279, 303, 1041, 1081, 1774, 2265
HpyCH4III	ACNGT	7 cut(s)	720, 1716, 1916, 2140, 2149, 2218, 2428
HpyCH4IV	ACGT	4 cut(s)	379, 1293, 1482, 1985
HpyF10VI	GCNNNNNNNGC	6 cut(s)	214, 356, 765, 836, 1316, 1593
HpySE526I	ACGT	4 cut(s)	379, 1293, 1482, 1985
Hsp92I	GRCGYC	1 cut(s)	1293
HspAI	GCGC	2 cut(s)	64, 759
KflI	GGGWCCC	1 cut(s)	190
Ksp22I	TGATCA	2 cut(s)	55, 1330
KspAI	GTTAAC	1 cut(s)	2385
LmnI	GCTCC	4 cut(s)	520, 932, 973, 1050
Lsp1109I	GCAGC	5 cut(s)	1087, 1751, 1943, 2253, 2386
LweI	GCATC	9 cut(s)	25, 253, 304, 550, 1208, 1605, 1888, 1951, 1996
MaeII	ACGT	4 cut(s)	379, 1293, 1482, 1985
MaeIII	GTNAC	5 cut(s)	141, 542, 958, 1649, 2202
MbiI	CCGCTC	1 cut(s)	935
MfeI	CAATTG	2 cut(s)	653, 2360
MflI	RGATCY	5 cut(s)	1322, 1408, 1675, 1942, 2107
MhlI	GDGCHC	5 cut(s)	631, 970, 1525, 1543, 1798
MlsI	TGGCCA	1 cut(s)	2043
MluNI	TGGCCA	1 cut(s)	2043
MlyI	GAGTC	3 cut(s)	547, 634, 2011
MmeI	TCCRAC	1 cut(s)	1326
Mox20I	TGGCCA	1 cut(s)	2043
MscI	TGGCCA	1 cut(s)	2043
MseI	TTAA	3 cut(s)	1083, 2142, 2384
MslI	CAYNNNNRTG	1 cut(s)	1130
Msp20I	TGGCCA	1 cut(s)	2043
MspA1I	CMGCKG	1 cut(s)	2399
MspI	CCGG	3 cut(s)	411, 724, 749
MspR9I	CCNGG	5 cut(s)	319, 711, 724, 994, 1864
MunI	CAATTG	2 cut(s)	653, 2360
Mva1269I	GAATGC	2 cut(s)	90, 217
MvaI	CCWGG	4 cut(s)	319, 711, 994, 1864
MvnI	CGCG	1 cut(s)	178
MwoI	GCNNNNNNNGC	6 cut(s)	214, 356, 765, 836, 1316, 1593
NciI	CCSGG	1 cut(s)	724
NcoI	CCATGG	2 cut(s)	1105, 1131
NdeI	CATATG	1 cut(s)	108
NmeAIII	GCCGAG	2 cut(s)	1419, 2377
NmuCI	GTSAC	3 cut(s)	141, 958, 2202
NruI	TCGCGA	1 cut(s)	178
NsbI	TGCGCA	1 cut(s)	65
NspI	RCATGY	3 cut(s)	1129, 1393, 1808
PaeR7I	CTCGAG	1 cut(s)	1147
PagI	TCATGA	2 cut(s)	556, 1455
PaqCI	CACCTGC	1 cut(s)	714
PciI	ACATGT	1 cut(s)	1804
PcsI	WCGNNNNNNNCGW	1 cut(s)	1299
PctI	GAATGC	2 cut(s)	90, 217
PfeI	GAWTC	8 cut(s)	473, 581, 735, 988, 1008, 1073, 1169, 2049
PflMI	CCANNNNNTGG	1 cut(s)	1558
PkrI	GCNGC	5 cut(s)	1102, 1766, 1933, 2243, 2401
PleI	GAGTC	3 cut(s)	547, 634, 2011
PpsI	GAGTC	3 cut(s)	547, 634, 2011
Ppu21I	YACGTR	1 cut(s)	1483
PpuMI	RGGWCCY	2 cut(s)	154, 190
PscI	ACATGT	1 cut(s)	1804
Psp124BI	GAGCTC	1 cut(s)	1543
Psp5II	RGGWCCY	2 cut(s)	154, 190
Psp6I	CCWGG	4 cut(s)	317, 709, 992, 1862
PspGI	CCWGG	4 cut(s)	317, 709, 992, 1862
PspPI	GGNCC	6 cut(s)	154, 190, 720, 854, 1493, 1640
PspPPI	RGGWCCY	2 cut(s)	154, 190
PspXI	VCTCGAGB	1 cut(s)	1147
PstI	CTGCAG	2 cut(s)	1769, 2134
PstNI	CAGNNNCTG	1 cut(s)	2074
PsuI	RGATCY	5 cut(s)	1322, 1408, 1675, 1942, 2107
RruI	TCGCGA	1 cut(s)	178
RsaI	GTAC	2 cut(s)	1239, 1718
RsaNI	GTAC	2 cut(s)	1238, 1717
RseI	CAYNNNNRTG	1 cut(s)	1130
SacI	GAGCTC	1 cut(s)	1543
SaqAI	TTAA	3 cut(s)	1083, 2142, 2384
SatI	GCNGC	5 cut(s)	1101, 1765, 1932, 2242, 2400
Sau96I	GGNCC	6 cut(s)	154, 190, 720, 854, 1493, 1640
SchI	GAGTC	3 cut(s)	547, 634, 2011
ScrFI	CCNGG	5 cut(s)	319, 711, 724, 994, 1864
SduI	GDGCHC	5 cut(s)	631, 970, 1525, 1543, 1798
SfaNI	GCATC	9 cut(s)	25, 253, 304, 550, 1208, 1605, 1888, 1951, 1996
SfcI	CTRYAG	5 cut(s)	834, 873, 888, 1765, 2130
Sfr274I	CTCGAG	1 cut(s)	1147
SinI	GGWCC	5 cut(s)	154, 190, 720, 854, 1493
SlaI	CTCGAG	1 cut(s)	1147
SmiMI	CAYNNNNRTG	1 cut(s)	1130
SmlI	CTYRAG	3 cut(s)	29, 894, 1147
SmoI	CTYRAG	3 cut(s)	29, 894, 1147
SpeI	ACTAGT	2 cut(s)	50, 1189
SsiI	CCGC	6 cut(s)	206, 527, 799, 935, 1354, 2397
SstI	GAGCTC	1 cut(s)	1543
StyD4I	CCNGG	5 cut(s)	317, 709, 722, 992, 1862
StyI	CCWWGG	4 cut(s)	267, 622, 1105, 1131
TaaI	ACNGT	7 cut(s)	720, 1716, 1916, 2140, 2149, 2218, 2428
TaiI	ACGT	4 cut(s)	382, 1296, 1485, 1988
TaqI	TCGA	9 cut(s)	307, 631, 1004, 1148, 1656, 1662, 1778, 1792, 2419
TatI	WGTACW	1 cut(s)	1716
TfiI	GAWTC	8 cut(s)	473, 581, 735, 988, 1008, 1073, 1169, 2049
Tru1I	TTAA	3 cut(s)	1083, 2142, 2384
Tru9I	TTAA	3 cut(s)	1083, 2142, 2384
TscAI	CASTG	4 cut(s)	424, 781, 1774, 1971
TseFI	GTSAC	3 cut(s)	141, 958, 2202
TseI	GCWGC	5 cut(s)	1100, 1764, 1931, 2241, 2399
Tsp45I	GTSAC	3 cut(s)	141, 958, 2202
TspDTI	ATGAA	3 cut(s)	650, 1472, 1986
TspRI	CASTG	4 cut(s)	424, 781, 1774, 1971
Van91I	CCANNNNNTGG	1 cut(s)	1558
VpaK11BI	GGWCC	5 cut(s)	154, 190, 720, 854, 1493
XapI	RAATTY	3 cut(s)	574, 1423, 2155
XbaI	TCTAGA	3 cut(s)	1535, 1945, 2020
XceI	RCATGY	3 cut(s)	1129, 1393, 1808
XhoI	CTCGAG	1 cut(s)	1147
ZraI	GACGTC	1 cut(s)	1294

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.