pycom05g31310

15.4 kDa class V heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014398
Physical Location & Seq
Reverse (-)
31093323 .. 31094060
738 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 417 bp
ATGGAGATCCCAGCCTTGCACCATTACCAATATTTCTTCCCTTCTCACCTCATCTACCCATATCATTTGGCACCAGAAAACTATGTGCACTGGACTGAGACCCCTGAGTCTCACATTTTCTCCGTTGACCTCCCCGGTGTGAGGAAAGAGGAGATAAAAGTACAAGTGGAGGACTCCGTATACCTCATAATTCGAACACAGAGGCTTGAAGAGGAGGAGGAGGAGTCCACAGAGCAGCCTAGCAGGACCTTCATGAGGAAATTCCTGATTCCGGCTGAGGCCGATATCGAACGGATTTCAGCCAGATACGAAGACGGAGTGTTGACGGTCACAGTGCCGAGATCTTTGAGGAGGGCTTTCTTCATTGAGCCAGCTGATGTGCCAGAGAGGCTTCAAGTTCTTGCAAGGGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

139

Amino Acids

16.27

Weight (kDa)

5.12

Isoelectric Point (pI)

75.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 31 - 116 3.1e-20 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21870
fragaria_vesca FvH4_3g02330
malus_domestica MD05G1343700.v1.1 MD10G1319400.v1.1
prunus_persica Prupe.4G023100_v2.0.a1
pyrus_communis pycom05g31310 pycom10g27030
rosa_chinensis RchiOBHm_Chr5g0003551
rosa_laevigata RLG00000031151
rosa_multiflora Rmu_sc0004279.1_g000008 Rmu_sc0009787.1_g000003
rosa_roxburghii Rroxscaffold_1G00072410
rosa_rugosa Rorug04G0404100
rosa_samantha Rh5AG031200 Rh5CG033700 Rh5DG030900
rosa_wichuraiana Rw5G002910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 70
AccI GTMKAC 1 cut(s) 180
AcsI RAATTY 1 cut(s) 260
AfaI GTAC 1 cut(s) 162
AfiI CCNNNNNNNGG 3 cut(s) 141, 255, 271
AgsI TTSAA 2 cut(s) 209, 395
AluBI AGCT 1 cut(s) 374
AluI AGCT 1 cut(s) 374
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 2 cut(s) 92, 114
Alw44I GTGCAC 1 cut(s) 86
AoxI GGCC 1 cut(s) 279
ApaLI GTGCAC 1 cut(s) 86
ApeKI GCWGC 2 cut(s) 235, 410
ApoI RAATTY 1 cut(s) 260
AspS9I GGNCC 1 cut(s) 246
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 1 cut(s) 38
AsuII TTCGAA 1 cut(s) 193
AvaII GGWCC 1 cut(s) 246
BaeGI GKGCMC 1 cut(s) 90
BanI GGYRCC 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 90
BbvCI CCTCAGC 1 cut(s) 276
BbvI GCAGC 2 cut(s) 247, 397
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 2 cut(s) 92, 114
BfaI CTAG 1 cut(s) 240
BglI GCCNNNNNGGC 1 cut(s) 388
BglII AGATCT 1 cut(s) 341
BisI GCNGC 2 cut(s) 236, 411
BlsI GCNGC 2 cut(s) 237, 412
Bme1390I CCNGG 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 246
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 193
BpuMI CCSGG 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 133
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 209, 239
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 255, 271
Bse1I ACTGG 1 cut(s) 95
BseDI CCNNGG 1 cut(s) 133
BseLI CCNNNNNNNGG 3 cut(s) 141, 255, 271
BseMII CTCAG 3 cut(s) 87, 96, 267
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 6 cut(s) 164, 227, 230, 233, 236, 364
BseSI GKGCMC 1 cut(s) 90
BseXI GCAGC 2 cut(s) 247, 397
BseYI CCCAGC 1 cut(s) 10
BshFI GGCC 1 cut(s) 281
BshNI GGYRCC 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 2 cut(s) 135, 272
BslI CCNNNNNNNGG 3 cut(s) 141, 255, 271
BsmAI GTCTC 2 cut(s) 92, 114
BsnI GGCC 1 cut(s) 281
Bso31I GGTCTC 1 cut(s) 92
Bsp119I TTCGAA 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 2 cut(s) 6, 341
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 3 cut(s) 88, 97, 268
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 72
BspT104I TTCGAA 1 cut(s) 193
BspT107I GGYRCC 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 92
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 2 cut(s) 6, 341
BssNAI GTATAC 1 cut(s) 181
Bst1107I GTATAC 1 cut(s) 181
Bst4CI ACNGT 2 cut(s) 328, 334
Bst6I CTCTTC 1 cut(s) 204
BstAPI GCANNNNNTGC 1 cut(s) 410
BstBI TTCGAA 1 cut(s) 193
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 3 cut(s) 96, 105, 276
BstENI CCTNNNNNAGG 1 cut(s) 253
BstKTI GATC 2 cut(s) 9, 344
BstMAI GTCTC 2 cut(s) 92, 114
BstMBI GATC 2 cut(s) 6, 341
BstMWI GCNNNNNNNGC 2 cut(s) 388, 410
BstSCI CCNGG 1 cut(s) 133
BstSLI GKGCMC 1 cut(s) 90
BstV1I GCAGC 2 cut(s) 247, 397
BstV2I GAAGAC 1 cut(s) 318
BstX2I RGATCY 2 cut(s) 6, 341
BstYI RGATCY 2 cut(s) 6, 341
BstZ17I GTATAC 1 cut(s) 181
BsuRI GGCC 1 cut(s) 281
BtsIMutI CAGTG 2 cut(s) 88, 339
Cac8I GCNNGC 1 cut(s) 372
CciI TCATGA 1 cut(s) 252
Cfr13I GGNCC 1 cut(s) 246
Csp6I GTAC 1 cut(s) 161
CviAII CATG 1 cut(s) 253
CviQI GTAC 1 cut(s) 161
DdeI CTNAG 3 cut(s) 96, 105, 276
DpnI GATC 2 cut(s) 8, 343
DpnII GATC 2 cut(s) 6, 341
DrdI GACNNNNNNGTC 1 cut(s) 106
DseDI GACNNNNNNGTC 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 92
Eco32I GATATC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 246
EcoNI CCTNNNNNAGG 1 cut(s) 253
EcoO109I RGGNCCY 1 cut(s) 246
EcoRV GATATC 1 cut(s) 286
FaeI CATG 1 cut(s) 256
FaiI YATR 5 cut(s) 61, 84, 181, 188, 254
FalI AAGNNNNNCTT 1 cut(s) 397
FatI CATG 1 cut(s) 252
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 2 cut(s) 236, 411
Fsp4HI GCNGC 2 cut(s) 236, 411
FspBI CTAG 1 cut(s) 240
GluI GCNGC 2 cut(s) 236, 411
GsaI CCCAGC 1 cut(s) 14
HaeIII GGCC 1 cut(s) 281
HapII CCGG 2 cut(s) 135, 272
Hin1II CATG 1 cut(s) 256
HincII GTYRAC 2 cut(s) 127, 324
HindII GTYRAC 2 cut(s) 127, 324
HinfI GANTC 4 cut(s) 107, 173, 224, 268
HpaII CCGG 2 cut(s) 135, 272
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 5 cut(s) 88, 127, 181, 228, 324
Hpy188III TCNNGA 2 cut(s) 253, 265
Hpy8I GTNNAC 5 cut(s) 88, 127, 181, 228, 324
HpyAV CCTTC 2 cut(s) 51, 259
HpyCH4III ACNGT 2 cut(s) 328, 334
HpyCH4V TGCA 3 cut(s) 19, 88, 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 388, 410
HpyF3I CTNAG 3 cut(s) 96, 105, 276
Hsp92II CATG 1 cut(s) 256
Kzo9I GATC 2 cut(s) 6, 341
Lsp1109I GCAGC 2 cut(s) 247, 397
MaeI CTAG 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 328
MalI GATC 2 cut(s) 8, 343
MboI GATC 2 cut(s) 6, 341
MboII GAAGA 4 cut(s) 28, 221, 323, 352
MflI RGATCY 2 cut(s) 6, 341
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 2 cut(s) 189, 260
MlyI GAGTC 3 cut(s) 116, 167, 233
MseI TTAA 1 cut(s) 415
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 2 cut(s) 135, 272
MspR9I CCNGG 1 cut(s) 135
MwoI GCNNNNNNNGC 2 cut(s) 388, 410
NciI CCSGG 1 cut(s) 135
NdeII GATC 2 cut(s) 6, 341
NlaIII CATG 1 cut(s) 256
NlaIV GGNNCC 1 cut(s) 72
NmeAIII GCCGAG 1 cut(s) 363
NmuCI GTSAC 1 cut(s) 328
NspV TTCGAA 1 cut(s) 193
PagI TCATGA 1 cut(s) 252
PfeI GAWTC 1 cut(s) 268
PkrI GCNGC 2 cut(s) 237, 412
PleI GAGTC 3 cut(s) 115, 167, 232
PpsI GAGTC 3 cut(s) 115, 167, 232
PpuMI RGGWCCY 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 246
PspFI CCCAGC 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 246
PspPPI RGGWCCY 1 cut(s) 246
PsuI RGATCY 2 cut(s) 6, 341
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 1 cut(s) 162
RsaNI GTAC 1 cut(s) 161
SaqAI TTAA 1 cut(s) 415
SatI GCNGC 2 cut(s) 236, 411
Sau3AI GATC 2 cut(s) 6, 341
Sau96I GGNCC 1 cut(s) 246
SchI GAGTC 3 cut(s) 116, 167, 233
ScrFI CCNGG 1 cut(s) 135
SduI GDGCHC 1 cut(s) 90
SetI ASST 5 cut(s) 51, 132, 186, 251, 376
SfuI TTCGAA 1 cut(s) 193
SinI GGWCC 1 cut(s) 246
Sse9I AATT 2 cut(s) 189, 260
SspI AATATT 1 cut(s) 32
SspMI CTAG 1 cut(s) 240
StyD4I CCNGG 1 cut(s) 133
TaaI ACNGT 2 cut(s) 328, 334
TaqI TCGA 2 cut(s) 193, 288
TasI AATT 2 cut(s) 189, 260
TatI WGTACW 1 cut(s) 160
TfiI GAWTC 1 cut(s) 268
Tru1I TTAA 1 cut(s) 415
Tru9I TTAA 1 cut(s) 415
TscAI CASTG 2 cut(s) 95, 339
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 2 cut(s) 235, 410
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 2 cut(s) 241, 352
TspGWI ACGGA 4 cut(s) 112, 166, 307, 330
TspRI CASTG 2 cut(s) 95, 339
VneI GTGCAC 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 246
XagI CCTNNNNNAGG 1 cut(s) 253
XapI RAATTY 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.