pycom05g32830

Sister chromatid cohesion protein PDS5 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009376
Physical Location & Seq
Forward (+)
32065656 .. 32075337
9682 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4377 bp
ATGAATGTTATAGAACAGTGCACAGGAAAGCTTGAATCTGGCATTAAACAATTTCTTATATCATCAATGTCAGGAGACAACAAGTCTGAGAATCATCAGATTGATTACCATGAAGTCATCTATGATGTATATCGTTGTGCTCCCCAAATCTTATCAGGAGTTGTCCCATACCTGACAGGAGAGTTACTGACTGATCAGCTAGAAACTCGTTTAAAAGCGGTTAGTTTAGTTGGAGACCTATTCTCTCTGCCAGGCTCAACCATCTTCGAAGCCTTTCAGCCAATCTTTTCAGAGTTCTTGAAGAGGTTGACTGATAGAGTTGTGGAGGTTCGCATGTTGGTCCTCCAACATGTCAAGAGCTGTATGCTGTCAAATCCTTTCAGAGCTGAAGCTTCCGAAATAATTTCTGCCCTCTGTGACCGGCTATTGGACTTTGAAGAAAAAGTACGAAAGCAAGTTGTTGCTGTAGTTTATGATGTGGCATGTCATGCCCTTAGTTCCATTCCACTAGAAACTATAAAACTGGTTGCAGAGCGCCTTCGAGATAAATCTCTACTTGTTAAAAAGTATACCATGGAGAGGTTAGCTGAGATATTCAGGGTTTATTGCGCAAAGTGCTCTGATGGTCCACTCCTATCCAGTGATTTTGATTGGATTCCTGGGAAGATTCTGAGATGTTTTTATGACAAAGATTTCAGATCTGATACAATTGAAAATGTTCTCTGCGAGTCCCTGTTCCCTACTAACTTTTCAGTAAAAGATAAAGTTAAACATTGGGTGAGGGTCTTCTCAAGATTTGATAAAGTTGAGGTTAAGGCTCTTGAGAAGATTCTGGAGCAGAAGCAAAGGTTACAACAAGAGATGCAGAAGTATCTGTCACTTAAGCAGATGCATCAGGATGGTGATGCTCCTGAGGTCCAAAAAAAAATTCTCTTTTGCTTCCGTATAATGTCACGGTTGTTTGCTGACCCAGTTAAGGCTGAAGAGAATTTTCAATTTCTTGATCAACTGAAAGACGTTAACATCTGGAAGATCTTAACAAATCTTGTTGATCCAAACACTGGCTCCCAGCAAGCTTGCACCTTACGGGATGATTTGCTTAGGATTCTAGGTGAAAAACACCGACTATATGATTTTCTTAGCACTTTCTCAGTGAAGTGTTCTTATCTACTTTTCAACAAAGAGCATGTAAAGGAAATTCTTCTGGAGGTTCTTGTACACAAATCTACCGCAGATATGAAATATGTGCAATCTTGCATGAATATTTTGGTGATTCTTGCACGCTTTAGTCCATTACTACTCAGTGGGACAGGAGAAGAACTAGTAAATTTGCTTAAAGACGATGATGAAACCATAAAAGAAGGGGTTTTGAATATTTTAGCAAAGGCTGGTGGTACCATCCGGGAGCATCTTGCAGTGTCATCAAGTTCCATAGACCTTGTATTGGAGAGGTTGTGTTTGGAGGGCAGTCGAAGACAGGCAAAGTATGCTGTACATGCCCTCGCAGCGATAACAAAGGATGATGGACTTAAATCACTCTCTGTTCTATACAAGAGGCTTGTGGATATGCTGGAGGAGAGGACACATTTGCCTGCTGTACTACAATCTCTGGGGTGTATAGCTCAGACTGCAATGCCAGTTTTTGAAACTAGAGAGAAGGAAATTGAAGAGTTTATAATTGAAAAGATCCTAAAGTGCAATAATAAATCAGGTGACTATAAAATTGCATCCTGGGATGACAAAAGTGATCTTTGTTTGCTAAAGATATACGGGATAAAGACATTAGTAAAAAGTTATTTGCCTGTCAAAGATGCTCATGTTCGCTCTGGCATTGATGGTCTGCTGGAAATCTTAAGAAACACACTTTCTTGTGGAGAAATATCAAAAGACATAGAAACAAGTGCAGTTGACAAGGCTCATTTGAGGCTTGCTTCTGCAAAGGCGATTCTTCGTCTGTCCAAGCACTGGAATCATAAGATACCTGTTGATATCTTCTACTTAACCTTGAAGACTTCAGAGATTAGTTTCCCTCAAGCTAAGAAAATTTTCCTGAACAAAGTTCATCAATACATGAAGGACCGGCTTTTAGATGGAAAATACGCTTGTGCATTCTTTTTTAACACATCTCGATCAAAGTCAGTGGAGTTTCAAGAGGAGAAACAGAATCTCGCAGACATTATTCAAATGTATCACCAAACAAAGGTGCGGCATCTCTCCATGCAGCAATCTGATGCCAATTCATTGACTGCTTATCCGGAGTACATCCTCCCATACTTGGTTCATGCCCTTGCACATCATTCTTGCCCTAATATTGATGAATGCAAGGATGTGAAAGCATTTGAGCCGATATACCGGCAATTGCACTTATTTCTGTCTATGTTGGTGCATAGAGATGATGATGTCAAGTCAGAATCTAGTAGCAACATTGAAAAGGAGGATATTTCTGCTATAATCTCTATCTTTCAAAGTATCAAGTGCTCTGAAGATATATATGATGCAACAAAGTCAAAGAATTCACATGGTATTTGTGACCTTGGGCTGTCAGCTACCAAGCGCTTATCCCCAAAGGAGTATGATTTACAAGGATTGCCTGCATCAGTTCCTCTACCTTCTATGTTGTACAAACAGTATGACAAGAAAGAAGGAGATGACTCTATGGCCAGTGAAGGGAAAACGTGGTTGGCCGATGACTCTGTGCTGGCTCACTTTGAATCTCTTAAGTTAGAGTCTAGTGAGATGGGACTTTCAGAGATTGCTGAAGATGAACTTATGAAAGATGGTGAAAGAGATGGAGGTGAAGTGCCTTTAGGAAGAATTATAAAGCGCTTAAGATCTCAAAATTCCAAGGCTAAAAAGGCAAAAAAGAACAAGGCATCATCAGCTGATGCAGAGAATGCTGAAAATAGTGTTGATATATTGAAAATGGTCAGGGATATAAATTTGGATAATTTGGAAAAGCCTTCTAAGTTTGTGTCAAGCAACGGTCATGAAAATCTTCCAAGCAAGCAATCAAGGATCGATCTAAAGCACGAAAAGGAAAACAAAAGAAAATCTACCGAGGCAACGTCTGTCCCCATTCCTAAACGTAGGAGGTCATCATCCGCTCATGGTGCTTTCAGATCACCTAGAAGTACTTCAAAGTCTCCTCTAAGTGCTTCAGTAGACGATGTAGACAATAGAAAACTGGTTCAAGGCACCGAGTCAGATTTGTTGGTTTCATGCTTCCGGAAGAATGCTACCTCCTCGTCACAACGTAAAGGCAGGGCCTCTGACCATGGTCTTAATGATGAGACAAATGAAGTTGGAGAAGCCAGTGACCATGAAGAGCCTAATGTGCTGGAGGCTGACAAGGAAGACCTAAATAGTGATTTCAACTCTCCTGCAGGATCCATCAAGAAGCGGAAAAGAAAGAGCATTTCTGGTTTGGCAAAGTGCAAATTCAAGGAAAGTGGAAAAGATGTCGAAGACTTGATTGGTTGCAGAATAAAAGTTTGGTGGCCAATGGATAAGCGATTTTATGAAGGCACAGTGAAGTCGTTCGATACGTTAAAGAGGAAGCATGTGATACTGTATGAAGATGGAGATGTAGAAGTACTACGGTTAGAAAAGGAACGGTGGGAGCTCATTGACAAAGGTCGCAAGCCCACAAAGAAGTTAAATTCGTCAAATAAATCCCCTTCAAAAGAAGTGTCCCCTGGTCAAAAGAATAAGCGCACAGGTGGTTCGCGAGAGAACAAAAAACCAATTAAAACAGTTAAAAGGAAAAGAACACCAAAGAAAAACTCGGCTGGGAAGAAGGGCGTATCAAAAAGAAATCATTGGGGAAGTAGGGAAAAAGAGAGTTCTGAAGTATCGAATGATGAGCCTGCCCTTACATCTAAAATTGATGAAATCGACTCAGGTAGTTCAGAAGAAGAGAATGCTGAAAAAGTGGACGAGAACGTGACAGATGAGGGGGAATCTGACACGGAAGTGAAGTTTGTTTCTGAAGGACAAGGGTTGAAAGATGCAGAAGAAAGTCCAAATAATACTGAGGAATCTGATGAAGAGAATACTGGTTCGGAAGGGATCCCTGCTGAAGACATGGACAGTATCCCACAAGATGCCGAAAATGGAAATGAGGAGATGCAACACTCAGAAGAGAAAGAAGCAGATGAACTAAGTGGAGGGTCAAGAGGGGTTAACGAGGAAGATGCGTCAGATTCTGAAAGGACTCAAGAAAAAGATGATATTTCTGGTAGTCCCTTGAAACAGGAGAAATCCCATGTAGAGCCTTCAAGTCTTTCTGATGCCGGAGATGACGAACTTTCTGATGATGAGCCTCTTGGCAAGTGGACGCAACGAGTAGTGAAAAAAGGTTCAAAGCGAGTGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1459

Amino Acids

164.58

Weight (kDa)

6.09

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDS5 PF20168 2 - 875 1.9e-195 Sister chromatid cohesion protein PDS5 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1676, 2819
Acc16I TGCGCA 1 cut(s) 610
Acc65I GGTACC 1 cut(s) 1394
AccB1I GGYRCC 2 cut(s) 1394, 3194
AccB7I CCANNNNNTGG 2 cut(s) 1061, 1965
AccBSI CCGCTC 1 cut(s) 3104
AccI GTMKAC 3 cut(s) 569, 3162, 3171
AccII CGCG 1 cut(s) 3727
AccIII TCCGGA 2 cut(s) 2254, 3225
AciI CCGC 5 cut(s) 218, 1230, 2206, 3102, 3400
AclWI GGATC 7 cut(s) 1046, 1681, 3023, 3381, 3394, 4063, 4076
AcoI YGGCCR 3 cut(s) 2658, 2682, 3497
AcuI CTGAAG 9 cut(s) 408, 1002, 1998, 2502, 2778, 3141, 3867, 4008, 4098
AfaI GTAC 9 cut(s) 447, 1218, 1396, 1494, 1599, 2261, 2621, 3133, 3594
AfeI AGCGCT 2 cut(s) 2555, 2825
AfiI CCNNNNNNNGG 9 cut(s) 427, 579, 976, 1061, 1444, 1965, 2200, 2275, 2667
AflII CTTAAG 4 cut(s) 881, 1852, 2717, 2827
AflIII ACRYGT 1 cut(s) 349
AhdI GACNNNNNGTC 1 cut(s) 82
AhlI ACTAGT 1 cut(s) 1321
AjnI CCWGG 4 cut(s) 250, 658, 1730, 3694
AjuI GAANNNNNNNTTGG 8 cut(s) 912, 944, 2663, 2695, 3456, 3475, 3488, 3507
AleI CACNNNNGTG 1 cut(s) 3971
Alw21I GWGCWC 5 cut(s) 23, 142, 620, 2480, 3624
Alw26I GTCTC 4 cut(s) 69, 228, 3147, 3284
Alw44I GTGCAC 1 cut(s) 19
AlwI GGATC 7 cut(s) 1046, 1681, 3023, 3381, 3394, 4063, 4076
AlwNI CAGNNNCTG 1 cut(s) 4205
Aor13HI TCCGGA 2 cut(s) 2254, 3225
Aor51HI AGCGCT 2 cut(s) 2555, 2825
AoxI GGCC 4 cut(s) 2658, 2682, 3264, 3497
ApaLI GTGCAC 1 cut(s) 19
ApeKI GCWGC 2 cut(s) 1505, 2221
ArsI GACNNNNNNTTYG 2 cut(s) 3674, 3706
Asp700I GAANNNNTTC 7 cut(s) 273, 717, 1200, 2045, 2994, 3133, 3536
Asp718I GGTACC 1 cut(s) 1394
AspLEI GCGC 5 cut(s) 537, 611, 2556, 2826, 3714
AspS9I GGNCC 5 cut(s) 340, 626, 916, 2077, 3264
AsuC2I CCSGG 1 cut(s) 1403
AsuHPI GGTGA 9 cut(s) 790, 914, 1124, 1282, 1724, 2183, 2792, 2807, 3114
AsuII TTCGAA 1 cut(s) 267
AvaII GGWCC 4 cut(s) 340, 626, 916, 2077
AxyI CCTNAGG 1 cut(s) 912
BaeGI GKGCMC 1 cut(s) 23
BalI TGGCCA 2 cut(s) 2660, 3499
BamHI GGATCC 2 cut(s) 3386, 4068
BanI GGYRCC 2 cut(s) 1394, 3194
BanII GRGCYC 1 cut(s) 3624
BarI GAAGNNNNNNTAC 2 cut(s) 429, 461
BbsI GAAGAC 6 cut(s) 778, 1480, 2015, 3360, 3471, 4086
Bbv12I GWGCWC 5 cut(s) 23, 142, 620, 2480, 3624
BbvI GCAGC 2 cut(s) 1517, 2233
BcgI CGANNNNNNTGC 2 cut(s) 4175, 4209
BciT130I CCWGG 4 cut(s) 252, 660, 1732, 3696
BciVI GTATCC 1 cut(s) 4103
BclI TGATCA 2 cut(s) 193, 1003
BcnI CCSGG 1 cut(s) 1403
BcoDI GTCTC 4 cut(s) 69, 228, 3147, 3284
BcuI ACTAGT 1 cut(s) 1321
BfaI CTAG 8 cut(s) 200, 509, 1109, 1322, 1650, 2415, 2730, 3126
BfmI CTRYAG 2 cut(s) 465, 3381
BfoI RGCGCY 3 cut(s) 538, 2557, 2827
BfrI CTTAAG 4 cut(s) 881, 1852, 2717, 2827
BfuI GTATCC 1 cut(s) 4103
BglII AGATCT 3 cut(s) 698, 1032, 2831
BisI GCNGC 3 cut(s) 1506, 2207, 2222
BlsI GCNGC 3 cut(s) 1507, 2208, 2223
BmcAI AGTACT 2 cut(s) 3133, 3594
Bme1390I CCNGG 5 cut(s) 252, 660, 1403, 1732, 3696
Bme18I GGWCC 4 cut(s) 340, 626, 916, 2077
BmeRI GACNNNNNGTC 1 cut(s) 82
BmgT120I GGNCC 5 cut(s) 340, 626, 916, 2077, 3264
BmiI GGNNCC 5 cut(s) 1066, 1396, 3196, 3388, 4070
BmrFI CCNGG 5 cut(s) 252, 660, 1403, 1732, 3696
BmrI ACTGGG 1 cut(s) 965
BmuI ACTGGG 1 cut(s) 965
BoxI GACNNNNGTC 2 cut(s) 3276, 3633
BpiI GAAGAC 6 cut(s) 778, 1480, 2015, 3360, 3471, 4086
BpmI CTGGAG 4 cut(s) 854, 1226, 1592, 3359
Bpu10I CCTNAGC 1 cut(s) 1100
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 4 cut(s) 775, 842, 2016, 4200
BpuMI CCSGG 1 cut(s) 1403
Bsa29I ATCGAT 1 cut(s) 3018
BsaBI GATNNNNATC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 228
BsaJI CCNNGG 8 cut(s) 573, 659, 1731, 2533, 2844, 3057, 3274, 3694
BsaWI WCCGGW 2 cut(s) 2254, 3225
BsaXI ACNNNNNCTCC 6 cut(s) 1049, 1079, 1439, 1469, 2784, 2814
Bsc4I CCNNNNNNNGG 9 cut(s) 427, 579, 976, 1061, 1444, 1965, 2200, 2275, 2667
Bse118I RCCGGY 3 cut(s) 420, 2079, 2352
Bse21I CCTNAGG 1 cut(s) 912
Bse3DI GCAATG 1 cut(s) 1638
Bse8I GATNNNNATC 1 cut(s) 129
BseAI TCCGGA 2 cut(s) 2254, 3225
BseBI CCWGG 4 cut(s) 252, 660, 1732, 3696
BseCI ATCGAT 1 cut(s) 3018
BseDI CCNNGG 8 cut(s) 573, 659, 1731, 2533, 2844, 3057, 3274, 3694
BseGI GGATG 9 cut(s) 904, 1096, 1398, 1525, 1727, 1741, 2262, 2332, 3098
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 9 cut(s) 427, 579, 976, 1061, 1444, 1965, 2200, 2275, 2667
BseMI GCAATG 1 cut(s) 1638
BseRI GAGGAG 5 cut(s) 1589, 2168, 3135, 3232, 4136
BseSI GKGCMC 1 cut(s) 23
BseXI GCAGC 2 cut(s) 1517, 2233
BseYI CCCAGC 2 cut(s) 1068, 3788
BsgI GTGCAG 1 cut(s) 1923
Bsh1236I CGCG 1 cut(s) 3727
BshFI GGCC 4 cut(s) 2660, 2684, 3266, 3499
BshNI GGYRCC 2 cut(s) 1394, 3194
BshVI ATCGAT 1 cut(s) 3018
BsiHKAI GWGCWC 5 cut(s) 23, 142, 620, 2480, 3624
BsiSI CCGG 7 cut(s) 421, 1402, 2080, 2255, 2353, 3226, 4293
BslFI GGGAC 7 cut(s) 149, 715, 1321, 2754, 3056, 3676, 4227
BslI CCNNNNNNNGG 9 cut(s) 427, 579, 976, 1061, 1444, 1965, 2200, 2275, 2667
BsmAI GTCTC 4 cut(s) 69, 228, 3147, 3284
BsmFI GGGAC 7 cut(s) 149, 715, 1321, 2754, 3056, 3676, 4227
BsmI GAATGC 5 cut(s) 2108, 2324, 2899, 3238, 3925
BsnI GGCC 4 cut(s) 2660, 2684, 3266, 3499
Bso31I GGTCTC 1 cut(s) 228
Bsp119I TTCGAA 1 cut(s) 267
Bsp1286I GDGCHC 5 cut(s) 23, 142, 620, 2480, 3624
Bsp13I TCCGGA 2 cut(s) 2254, 3225
Bsp1407I TGTACA 3 cut(s) 1216, 1492, 2619
Bsp19I CCATGG 2 cut(s) 573, 3274
Bsp68I TCGCGA 1 cut(s) 3727
BspACI CCGC 5 cut(s) 218, 1230, 2206, 3102, 3400
BspANI GGCC 4 cut(s) 2660, 2684, 3266, 3499
BspDI ATCGAT 1 cut(s) 3018
BspEI TCCGGA 2 cut(s) 2254, 3225
BspFNI CGCG 1 cut(s) 3727
BspHI TCATGA 1 cut(s) 2986
BspLI GGNNCC 5 cut(s) 1066, 1396, 3196, 3388, 4070
BspMAI CTGCAG 1 cut(s) 3385
BspPI GGATC 7 cut(s) 1046, 1681, 3023, 3381, 3394, 4063, 4076
BspQI GCTCTTC 1 cut(s) 3318
BspT104I TTCGAA 1 cut(s) 267
BspT107I GGYRCC 2 cut(s) 1394, 3194
BspTI CTTAAG 4 cut(s) 881, 1852, 2717, 2827
BspTNI GGTCTC 1 cut(s) 228
BsrBI CCGCTC 1 cut(s) 3104
BsrDI GCAATG 1 cut(s) 1638
BsrFI RCCGGY 3 cut(s) 420, 2079, 2352
BsrGI TGTACA 3 cut(s) 1216, 1492, 2619
BssAI RCCGGY 3 cut(s) 420, 2079, 2352
BssECI CCNNGG 8 cut(s) 573, 659, 1731, 2533, 2844, 3057, 3274, 3694
BssNAI GTATAC 1 cut(s) 570
BssT1I CCWWGG 4 cut(s) 573, 2533, 2844, 3274
Bst1107I GTATAC 1 cut(s) 570
Bst2UI CCWGG 4 cut(s) 252, 660, 1732, 3696
Bst6I CTCTTC 7 cut(s) 296, 978, 1662, 3318, 3909, 4041, 4134
BstAFI CTTAAG 4 cut(s) 881, 1852, 2717, 2827
BstAPI GCANNNNNTGC 3 cut(s) 488, 1487, 2894
BstAUI TGTACA 3 cut(s) 1216, 1492, 2619
BstBI TTCGAA 1 cut(s) 267
BstDSI CCRYGG 2 cut(s) 573, 3274
BstF5I GGATG 9 cut(s) 904, 1096, 1398, 1525, 1727, 1741, 2262, 2332, 3098
BstFNI CGCG 1 cut(s) 3727
BstH2I RGCGCY 3 cut(s) 538, 2557, 2827
BstHHI GCGC 5 cut(s) 537, 611, 2556, 2826, 3714
BstMAI GTCTC 4 cut(s) 69, 228, 3147, 3284
BstNI CCWGG 4 cut(s) 252, 660, 1732, 3696
BstNSI RCATGY 6 cut(s) 337, 353, 486, 1190, 1499, 3563
BstPAI GACNNNNGTC 2 cut(s) 3276, 3633
BstSCI CCNGG 5 cut(s) 250, 658, 1401, 1730, 3694
BstSFI CTRYAG 2 cut(s) 465, 3381
BstSLI GKGCMC 1 cut(s) 23
BstUI CGCG 1 cut(s) 3727
BstV1I GCAGC 2 cut(s) 1517, 2233
BstV2I GAAGAC 6 cut(s) 778, 1480, 2015, 3360, 3471, 4086
BstX2I RGATCY 6 cut(s) 698, 1032, 1686, 2831, 3386, 4068
BstYI RGATCY 6 cut(s) 698, 1032, 1686, 2831, 3386, 4068
BstZ17I GTATAC 1 cut(s) 570
Bsu15I ATCGAT 1 cut(s) 3018
Bsu36I CCTNAGG 1 cut(s) 912
BsuI GTATCC 1 cut(s) 4103
BsuRI GGCC 4 cut(s) 2660, 2684, 3266, 3499
BsuTUI ATCGAT 1 cut(s) 3018
BtgI CCRYGG 2 cut(s) 573, 3274
BtsCI GGATG 9 cut(s) 904, 1096, 1398, 1525, 1727, 1741, 2262, 2332, 3098
BtsI GCAGTG 1 cut(s) 1422
BtuMI TCGCGA 1 cut(s) 3727
CaiI CAGNNNCTG 1 cut(s) 4205
CciI TCATGA 1 cut(s) 2986
CfoI GCGC 5 cut(s) 537, 611, 2556, 2826, 3714
Cfr10I RCCGGY 3 cut(s) 420, 2079, 2352
Cfr13I GGNCC 5 cut(s) 340, 626, 916, 2077, 3264
ClaI ATCGAT 1 cut(s) 3018
CseI GACGC 2 cut(s) 4185, 4345
Csp6I GTAC 9 cut(s) 446, 1217, 1395, 1493, 1598, 2260, 2620, 3132, 3593
CspCI CAANNNNNGTGG 4 cut(s) 2121, 2156, 3430, 3465
CviQI GTAC 9 cut(s) 446, 1217, 1395, 1493, 1598, 2260, 2620, 3132, 3593
DraI TTTAAA 1 cut(s) 213
DriI GACNNNNNGTC 1 cut(s) 82
EaeI YGGCCR 3 cut(s) 2658, 2682, 3497
Eam1104I CTCTTC 7 cut(s) 296, 978, 1662, 3318, 3909, 4041, 4134
Eam1105I GACNNNNNGTC 1 cut(s) 82
EarI CTCTTC 7 cut(s) 296, 978, 1662, 3318, 3909, 4041, 4134
Ecl136II GAGCTC 1 cut(s) 3622
Eco130I CCWWGG 4 cut(s) 573, 2533, 2844, 3274
Eco24I GRGCYC 1 cut(s) 3624
Eco31I GGTCTC 1 cut(s) 228
Eco32I GATATC 1 cut(s) 1990
Eco47I GGWCC 4 cut(s) 340, 626, 916, 2077
Eco47III AGCGCT 2 cut(s) 2555, 2825
Eco53kI GAGCTC 1 cut(s) 3622
Eco57I CTGAAG 9 cut(s) 408, 1002, 1998, 2502, 2778, 3141, 3867, 4008, 4098
Eco81I CCTNAGG 1 cut(s) 912
EcoICRI GAGCTC 1 cut(s) 3622
EcoO109I RGGNCCY 1 cut(s) 3264
EcoRI GAATTC 1 cut(s) 2512
EcoRII CCWGG 4 cut(s) 250, 658, 1730, 3694
EcoRV GATATC 1 cut(s) 1990
EcoT14I CCWWGG 4 cut(s) 573, 2533, 2844, 3274
EcoT22I ATGCAT 1 cut(s) 894
EcoT38I GRGCYC 1 cut(s) 3624
ErhI CCWWGG 4 cut(s) 573, 2533, 2844, 3274
FalI AAGNNNNNCTT 2 cut(s) 2751, 2783
FaqI GGGAC 7 cut(s) 149, 715, 1321, 2754, 3056, 3676, 4227
FbaI TGATCA 2 cut(s) 193, 1003
FblI GTMKAC 3 cut(s) 569, 3162, 3171
Fnu4HI GCNGC 3 cut(s) 1506, 2207, 2222
FokI GGATG 9 cut(s) 911, 1103, 1385, 1532, 1714, 1748, 2249, 2339, 3085
FriOI GRGCYC 1 cut(s) 3624
Fsp4HI GCNGC 3 cut(s) 1506, 2207, 2222
FspBI CTAG 8 cut(s) 200, 509, 1109, 1322, 1650, 2415, 2730, 3126
FspI TGCGCA 1 cut(s) 610
GlaI GCGC 5 cut(s) 536, 610, 2555, 2825, 3713
GluI GCNGC 3 cut(s) 1506, 2207, 2222
GsaI CCCAGC 2 cut(s) 1072, 3792
GsuI CTGGAG 4 cut(s) 854, 1226, 1592, 3359
HaeII RGCGCY 3 cut(s) 538, 2557, 2827
HaeIII GGCC 4 cut(s) 2660, 2684, 3266, 3499
HapII CCGG 7 cut(s) 421, 1402, 2080, 2255, 2353, 3226, 4293
HgaI GACGC 2 cut(s) 4185, 4345
HhaI GCGC 5 cut(s) 537, 611, 2556, 2826, 3714
Hin6I GCGC 5 cut(s) 535, 609, 2554, 2824, 3712
HinP1I GCGC 5 cut(s) 535, 609, 2554, 2824, 3712
HincII GTYRAC 4 cut(s) 309, 1021, 1909, 4183
HindII GTYRAC 4 cut(s) 309, 1021, 1909, 4183
HindIII AAGCTT 3 cut(s) 29, 390, 1074
HpaI GTTAAC 2 cut(s) 1021, 4183
HpaII CCGG 7 cut(s) 421, 1402, 2080, 2255, 2353, 3226, 4293
HphI GGTGA 9 cut(s) 790, 914, 1124, 1282, 1724, 2183, 2792, 2807, 3114
HpyCH4IV ACGT 7 cut(s) 1017, 2675, 3065, 3085, 3253, 3545, 3942
HpySE526I ACGT 7 cut(s) 1017, 2675, 3065, 3085, 3253, 3545, 3942
HspAI GCGC 5 cut(s) 535, 609, 2554, 2824, 3712
Kpn2I TCCGGA 2 cut(s) 2254, 3225
KpnI GGTACC 1 cut(s) 1398
Ksp22I TGATCA 2 cut(s) 193, 1003
KspAI GTTAAC 2 cut(s) 1021, 4183
LguI GCTCTTC 1 cut(s) 3318
LmnI GCTCC 6 cut(s) 145, 835, 913, 1070, 1405, 3619
Lsp1109I GCAGC 2 cut(s) 1517, 2233
MaeI CTAG 8 cut(s) 200, 509, 1109, 1322, 1650, 2415, 2730, 3126
MaeII ACGT 7 cut(s) 1017, 2675, 3065, 3085, 3253, 3545, 3942
MbiI CCGCTC 1 cut(s) 3104
MfeI CAATTG 2 cut(s) 708, 2357
MflI RGATCY 6 cut(s) 698, 1032, 1686, 2831, 3386, 4068
MhlI GDGCHC 5 cut(s) 23, 142, 620, 2480, 3624
MlsI TGGCCA 2 cut(s) 2660, 3499
MluNI TGGCCA 2 cut(s) 2660, 3499
MlyI GAGTC 7 cut(s) 737, 2645, 2684, 2735, 3209, 3890, 4207
MmeI TCCRAC 3 cut(s) 211, 370, 3283
Mox20I TGGCCA 2 cut(s) 2660, 3499
Mph1103I ATGCAT 1 cut(s) 894
MroI TCCGGA 2 cut(s) 2254, 3225
MroXI GAANNNNTTC 7 cut(s) 273, 717, 1200, 2045, 2994, 3133, 3536
MscI TGGCCA 2 cut(s) 2660, 3499
MslI CAYNNNNRTG 3 cut(s) 897, 2391, 3971
Msp20I TGGCCA 2 cut(s) 2660, 3499
MspA1I CMGCKG 1 cut(s) 2882
MspCI CTTAAG 4 cut(s) 881, 1852, 2717, 2827
MspI CCGG 7 cut(s) 421, 1402, 2080, 2255, 2353, 3226, 4293
MspR9I CCNGG 5 cut(s) 252, 660, 1403, 1732, 3696
MunI CAATTG 2 cut(s) 708, 2357
Mva1269I GAATGC 5 cut(s) 2108, 2324, 2899, 3238, 3925
MvaI CCWGG 4 cut(s) 252, 660, 1732, 3696
MvnI CGCG 1 cut(s) 3727
NciI CCSGG 1 cut(s) 1403
NcoI CCATGG 2 cut(s) 573, 3274
NlaIV GGNNCC 5 cut(s) 1066, 1396, 3196, 3388, 4070
NmeAIII GCCGAG 1 cut(s) 3764
NmuCI GTSAC 8 cut(s) 416, 876, 951, 1712, 2528, 3246, 3314, 3943
NruI TCGCGA 1 cut(s) 3727
NsbI TGCGCA 1 cut(s) 610
NsiI ATGCAT 1 cut(s) 894
NspI RCATGY 6 cut(s) 337, 353, 486, 1190, 1499, 3563
NspV TTCGAA 1 cut(s) 267
OliI CACNNNNGTG 1 cut(s) 3971
PagI TCATGA 1 cut(s) 2986
PciI ACATGT 1 cut(s) 349
PciSI GCTCTTC 1 cut(s) 3318
PcsI WCGNNNNNNNCGW 1 cut(s) 3542
PctI GAATGC 5 cut(s) 2108, 2324, 2899, 3238, 3925
PdmI GAANNNNTTC 7 cut(s) 273, 717, 1200, 2045, 2994, 3133, 3536
PflMI CCANNNNNTGG 2 cut(s) 1061, 1965
PfoI TCCNGGA 1 cut(s) 1401
PkrI GCNGC 3 cut(s) 1507, 2208, 2223
PleI GAGTC 7 cut(s) 736, 2645, 2684, 2734, 3208, 3890, 4207
PpsI GAGTC 7 cut(s) 736, 2645, 2684, 2734, 3208, 3890, 4207
PscI ACATGT 1 cut(s) 349
PshAI GACNNNNGTC 2 cut(s) 3276, 3633
PsiI TTATAA 2 cut(s) 1676, 2819
Psp124BI GAGCTC 1 cut(s) 3624
Psp6I CCWGG 4 cut(s) 250, 658, 1730, 3694
PspFI CCCAGC 2 cut(s) 1068, 3788
PspGI CCWGG 4 cut(s) 250, 658, 1730, 3694
PspN4I GGNNCC 5 cut(s) 1066, 1396, 3196, 3388, 4070
PspPI GGNCC 5 cut(s) 340, 626, 916, 2077, 3264
PstI CTGCAG 1 cut(s) 3385
PstNI CAGNNNCTG 1 cut(s) 4205
PsuI RGATCY 6 cut(s) 698, 1032, 1686, 2831, 3386, 4068
PvuII CAGCTG 1 cut(s) 2882
RruI TCGCGA 1 cut(s) 3727
RsaI GTAC 9 cut(s) 447, 1218, 1396, 1494, 1599, 2261, 2621, 3133, 3594
RsaNI GTAC 9 cut(s) 446, 1217, 1395, 1493, 1598, 2260, 2620, 3132, 3593
RseI CAYNNNNRTG 3 cut(s) 897, 2391, 3971
SacI GAGCTC 1 cut(s) 3624
SapI GCTCTTC 1 cut(s) 3318
SatI GCNGC 3 cut(s) 1506, 2207, 2222
Sau96I GGNCC 5 cut(s) 340, 626, 916, 2077, 3264
SbfI CCTGCAGG 1 cut(s) 3385
ScaI AGTACT 2 cut(s) 3133, 3594
SchI GAGTC 7 cut(s) 737, 2645, 2684, 2735, 3209, 3890, 4207
ScrFI CCNGG 5 cut(s) 252, 660, 1403, 1732, 3696
SdaI CCTGCAGG 1 cut(s) 3385
SduI GDGCHC 5 cut(s) 23, 142, 620, 2480, 3624
SfcI CTRYAG 2 cut(s) 465, 3381
SfuI TTCGAA 1 cut(s) 267
SinI GGWCC 4 cut(s) 340, 626, 916, 2077
SmiMI CAYNNNNRTG 3 cut(s) 897, 2391, 3971
SmlI CTYRAG 8 cut(s) 790, 821, 881, 1852, 2031, 2717, 2827, 4215
SmoI CTYRAG 8 cut(s) 790, 821, 881, 1852, 2031, 2717, 2827, 4215
SpeI ACTAGT 1 cut(s) 1321
Sse8387I CCTGCAGG 1 cut(s) 3385
SsiI CCGC 5 cut(s) 218, 1230, 2206, 3102, 3400
SspI AATATT 3 cut(s) 1264, 1375, 2311
SspMI CTAG 8 cut(s) 200, 509, 1109, 1322, 1650, 2415, 2730, 3126
SstI GAGCTC 1 cut(s) 3624
StyD4I CCNGG 5 cut(s) 250, 658, 1401, 1730, 3694
StyI CCWWGG 4 cut(s) 573, 2533, 2844, 3274
TaiI ACGT 7 cut(s) 1020, 2678, 3068, 3088, 3256, 3548, 3945
TaqI TCGA 9 cut(s) 267, 541, 1471, 2128, 3018, 3462, 3540, 3854, 3894
TatI WGTACW 7 cut(s) 1216, 1492, 1597, 2259, 2619, 3131, 3592
TauI GCSGC 1 cut(s) 2209
TseFI GTSAC 8 cut(s) 416, 876, 951, 1712, 2528, 3246, 3314, 3943
TseI GCWGC 2 cut(s) 1505, 2221
Tsp45I GTSAC 8 cut(s) 416, 876, 951, 1712, 2528, 3246, 3314, 3943
TspGWI ACGGA 2 cut(s) 932, 3983
Van91I CCANNNNNTGG 2 cut(s) 1061, 1965
Vha464I CTTAAG 4 cut(s) 881, 1852, 2717, 2827
VneI GTGCAC 1 cut(s) 19
VpaK11BI GGWCC 4 cut(s) 340, 626, 916, 2077
XceI RCATGY 6 cut(s) 337, 353, 486, 1190, 1499, 3563
XmiI GTMKAC 3 cut(s) 569, 3162, 3171
XmnI GAANNNNTTC 7 cut(s) 273, 717, 1200, 2045, 2994, 3133, 3536
XspI CTAG 8 cut(s) 200, 509, 1109, 1322, 1650, 2415, 2730, 3126
ZrmI AGTACT 2 cut(s) 3133, 3594
Zsp2I ATGCAT 1 cut(s) 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.