Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0006478

Physical Location & Seq

Genomic Coordinates Reverse (-)

707813 .. 719423

Gene Structure 11611 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2961 bp

ATGGCCCTCCGCCGTCCGGGGCTCCTCCTCCCCCTCTCTTTGTTCCAGGTGTTATTTATTCTGGGCGTAGCATATGCTGCTCAGACCTCCGTGACGTCTAATGCTACGGCGGCGGGGGAAAGACATTCGGAAGGGTATTGCGCAATGTATGGCATTTGCGGGCAACGTAGTGATGGAAAGTACATAAACTGCCCTTTCGGTACCCCATCCGTTAAGCCGGATGATTTACTTTCGTCCAAGGTCCAAAGTCTCTGTCCAACAATTACTGAAAATGTTTGTTGTACAGAAGCTCAATTTGACACACTACGGGCACAGGTCCAGCAAGCGATTCCCTTTCTTGTAGGTTGTCCGGCATGCTTAAGAAACTTTTTGAATTTGTTTTGTGAGCTTACCTGCTCTCCAAATCAGAGTTTATTTATCAATGTGACTTCTGTAGCCAAGGTTAACAGAAACATGACTGTCGATGGGATTGATTATTATATAACTGATGACTTTGGTGAAGGGTTATTTAACTCCTGCAAGGATGTCAAGTTCGGTACCATGAATTCTCGAGCTATAGAATTTATTGGTGCTGGTGCTAAAAATTTTAAAGACTGGTTTGCTTTTATCGGCAGACAAGCACCTCCTAATATGCCAGGTTCTCCATATGCCATTAGATTCCCTTCAAGTGTTACTGAGTCATCTGGGATGAAACCTATGAATGTATCTACTTATTCATGCGGTGATAATTCACTGGGCTGTTCCTGTGGTGATTGCCCTTCAGCTACAGTTTGTTCTAACACAGTTTCTCCGGTTTCCCGAAAAGGAGGTTCATGTTCAGTGAGAATTGGATCGATTAAGGCTAAATGTATTGACCTTGCTGTTGCTGTTCTATATATTGCACTGGTTTCTGTGTTTTTTGGGTGGGTTTTGTTTCATCGGACTAGAAAAACCAAGCCAGCTTCTGGGACAAAACCATGGTGGAGTGCAATGGATGATGGCGAAGTTCATTCTAACAGGGAGAAGAATGAGAACCCTCCGATGCAGGTATTTGAAGATGCTCCTCACGTTAGAAATGATGTCCAACTTTCAATTGTGCAAGGATACATTTCAAAATTTTTCAGGAGATATGGAACATGGGTTGCTAGGAATCCAATTATAGTGTTGTGCTCATCATTGGCTGTAGTTCTTCTGCTTTGTTTGGGTCTTATCCGTTTCAAGGTGGAAACAAGGCCTGAAAAGCTGTGGGTAGGGCCTGGGAGTAAAGCAGCAGAAGAGAAGCAATTTTTTGATAGCCACCTAGCCCCTTTCTACAGAATTGAGCAGCTAATATTAGCAACAATTCCAGAGGGTAAGCATGGTAACTCACCTAGTATTGTGACAGAGGACAATATTAAGTTACTTTTTGAGATACAGAAGAAGGTTGATGGAATTAAAGCAGATTATTCTGGATCCGTGGTATCTCTTGCTGATATTTGCATGAAGCCAATGGACAAAGATTGCGCCACTCAAAGTGTCCTACAGTACTTCAAAATGGATCCTGCAAATTATGATGGTTATGGTGGGGTTGAACACCTAAAGTATTGTTTTGAGCACTATTCATCTGCTGACACATGTATGAGTGCATTTAAAGGTCCTCTTGATCCTAGCACAGCATTAGGAGGCTTCTCTGGGAAAAACTACTCTGAGGCGTCAGCATTTCTTGTAACTTATCCTGTAAACAATGCTCTTAGTAATGATGGGAATGAAACTCAAAGAGCAGTGGCATGGGAGAAAGCCTTTATTGAGCTTGCAAAGGATGAATTGTTGCAGATGGTGCAATCTAGAAACTTAACACTTTCTTTTTCATCGGAAAGTTCTGTTGAAGAAGAACTTAAAAGAGAAAGTACAGCAGATGCTATTACAATATTGATAAGCTATCTTGTGATGTTTGCTTATATATCTCTTACTTTGGGTGATTCACCTCGTCTATCATCATTTTACATTTCGTCTAAGGTATTGCTTGGTCTCTCTGGGGTTGTGCTTGTCATGCTGTCAGTTCTTGGATCGGTGGGATTTTTCAGTGTTATAGGTGTAAAATCTACCTTAATCATCATGGAAGTTATTCCATTTCTTGTTCTAGCTGTTGGGGTAGATAATATGTGTATATTGGTGAATGCTGTCAAACGGCAACCCTTGGAGTTACCTTTAGAAGGGCGAATAAGCAATGCACTCGTGGAAGTTGGACCATCAATAACACTGGCTAGTCTATCAGAGGTCTTAGCATTTGCAGTTGGAAGTTTTATTCCTATGCCAGCATGCCGCGTGTTCTCCATGTTTGCAGCACTTGCTGTTCTGCTGGACTTTCTCCTTCAAGTTACTGCATTTGTAGCTTTGATTGTTTTTGATTTTAGGAGAACTGAGGATAAGAGGGTTGATTGTTTCCCATGCATGAAAATTTCATCATATGCCAACTCTGACAAAGGTATTGATCAGAAGAATCCTGGTTTATTGACTCGTTATATGAAGGAGATCCATGCTCCCATTCTCAGTCTTTGGGGAGTCAAGATAGTTGTCATTTCCATCTTTGTTGCATTTGCATTGGCTAGTATTGCATTATGCACCAGAATTCAGCCTGGTTTGGAACAAAAGATTGTTCTTCCCCGAGACTCCTATCTTCAGGGGTATTTCAATAATGTTTCAGAGTATCTCCGAATTGGACCCCCACTATACTTTGTTGTGAAGAACTTCAATTATAGTTCAGAATCAAGACATACGAACCAGTTGTGCTCAATTAGCCAGTGTGATTCAGATTCTCTTTTGAATGAGATTGCAAGAGCATCCTTAAATCCAGAATCAAGTTACATTGCTAAGCCTGCTGCTTCATGGCTTGATGATTTTCTTGTGTGGATATCTCCAGAAGCATTTGGGTGCTGTCGGAAGTTTACAGATGGGACTTACTGCCCTCCAGATCGATCAGCCACCTTGCTGCTCTCCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

987

Amino Acids

108.12

Weight (kDa)

6.42

Isoelectric Point (pI)

41.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	45 - 274	8.8e-66	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	420 - 611	5.3e-37	NPC1, middle luminal domain
Patched	PF02460	634 - 787	1.2e-31	Patched SSD
Sterol-sensing	PF12349	655 - 805	1.3e-48	Sterol-sensing domain of SREBP cleavage-activation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006478)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42470
fragaria_vesca	FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290
malus_domestica	MD06G1009400.v1.1
prunus_persica	Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1
pyrus_communis	pycom06g00780 pycom16g25890
rosa_chinensis	RchiOBHm_Chr7g0215501
rosa_laevigata	RLG00000002657
rosa_multiflora	Rmu_sc0005928.1_g000005
rosa_roxburghii	Rroxscaffold_3G00244190
rosa_rugosa	Rorug07G0154100 Rorug07G0154200
rosa_samantha	Rh7AG290300 Rh7BG282300 Rh7CG310300 Rh7DG294100
rosa_wichuraiana	Rw7G024790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	98
Acc16I	TGCGCA	1 cut(s)	142
Acc36I	ACCTGC	2 cut(s)	401, 1015
Acc65I	GGTACC	2 cut(s)	200, 536
AccB1I	GGYRCC	2 cut(s)	200, 536
AccB7I	CCANNNNNTGG	1 cut(s)	944
AccII	CGCG	1 cut(s)	2283
AciI	CCGC	6 cut(s)	10, 110, 113, 159, 720, 2281
AclWI	GGATC	8 cut(s)	838, 1425, 1438, 1511, 1524, 1616, 2032, 2485
AcsI	RAATTY	7 cut(s)	373, 544, 560, 583, 1094, 2415, 2586
AcuI	CTGAAG	2 cut(s)	744, 2621
AcyI	GRCGYC	2 cut(s)	95, 1670
AfaI	GTAC	6 cut(s)	182, 202, 283, 538, 1505, 1867
AfiI	CCNNNNNNNGG	4 cut(s)	16, 944, 1198, 2171
AflII	CTTAAG	1 cut(s)	358
AflIII	ACRYGT	1 cut(s)	1592
AjnI	CCWGG	5 cut(s)	45, 634, 1234, 2461, 2593
AloI	GAACNNNNNNTCC	2 cut(s)	515, 547
Alw21I	GWGCWC	3 cut(s)	1151, 1575, 2750
Alw26I	GTCTC	3 cut(s)	254, 1991, 2619
AlwI	GGATC	8 cut(s)	838, 1425, 1438, 1511, 1524, 1616, 2032, 2485
AlwNI	CAGNNNCTG	1 cut(s)	944
Ama87I	CYCGRG	2 cut(s)	549, 2622
AoxI	GGCC	3 cut(s)	3, 1211, 1232
ApeKI	GCWGC	6 cut(s)	77, 1247, 1303, 2300, 2837, 2947
ApoI	RAATTY	7 cut(s)	373, 544, 560, 583, 1094, 2415, 2586
ArsI	GACNNNNNNTTYG	2 cut(s)	238, 270
Asp700I	GAANNNNTTC	2 cut(s)	2082, 2705
Asp718I	GGTACC	2 cut(s)	200, 536
AspLEI	GCGC	2 cut(s)	143, 1484
AspS9I	GGNCC	7 cut(s)	4, 241, 316, 1232, 1613, 2204, 2678
AsuC2I	CCSGG	1 cut(s)	18
AsuHPI	GGTGA	7 cut(s)	509, 734, 761, 1338, 1932, 1946, 2143
AvaI	CYCGRG	2 cut(s)	549, 2622
AvaII	GGWCC	5 cut(s)	241, 316, 1613, 2204, 2678
BaeGI	GKGCMC	1 cut(s)	313
BamHI	GGATCC	2 cut(s)	1430, 1516
BanI	GGYRCC	2 cut(s)	200, 536
BanII	GRGCYC	1 cut(s)	24
BauI	CACGAG	1 cut(s)	2192
Bbv12I	GWGCWC	3 cut(s)	1151, 1575, 2750
BbvI	GCAGC	6 cut(s)	64, 1259, 1315, 2312, 2824, 2934
BceAI	ACGGC	2 cut(s)	123, 2162
BcgI	CGANNNNNNTGC	2 cut(s)	2173, 2207
BciT130I	CCWGG	5 cut(s)	47, 636, 1236, 2463, 2595
BciVI	GTATCC	1 cut(s)	1076
BclI	TGATCA	1 cut(s)	2449
BcnI	CCSGG	1 cut(s)	18
BcoDI	GTCTC	3 cut(s)	254, 1991, 2619
BfaI	CTAG	9 cut(s)	924, 1125, 1280, 1350, 1626, 1803, 2099, 2223, 2565
BfmI	CTRYAG	6 cut(s)	432, 555, 765, 1161, 1291, 1499
BfrI	CTTAAG	1 cut(s)	358
BfuAI	ACCTGC	2 cut(s)	401, 1015
BfuI	GTATCC	1 cut(s)	1076
BisI	GCNGC	8 cut(s)	78, 111, 1248, 1304, 2281, 2301, 2838, 2948
BlpI	GCTNAGC	1 cut(s)	2829
BlsI	GCNGC	8 cut(s)	79, 112, 1249, 1305, 2282, 2302, 2839, 2949
BmcAI	AGTACT	1 cut(s)	1505
Bme1390I	CCNGG	6 cut(s)	18, 47, 636, 1236, 2463, 2595
Bme18I	GGWCC	5 cut(s)	241, 316, 1613, 2204, 2678
BmeT110I	CYCGRG	2 cut(s)	549, 2622
BmgT120I	GGNCC	7 cut(s)	4, 241, 316, 1232, 1613, 2204, 2678
BmiI	GGNNCC	6 cut(s)	23, 202, 538, 1432, 1518, 2680
BmrFI	CCNGG	6 cut(s)	18, 47, 636, 1236, 2463, 2595
BmrI	ACTGGG	2 cut(s)	743, 2950
BmsI	GCATC	4 cut(s)	1011, 1027, 1864, 2807
BmuI	ACTGGG	2 cut(s)	743, 2950
BpmI	CTGGAG	2 cut(s)	2859, 2910
Bpu1102I	GCTNAGC	1 cut(s)	2829
BpuMI	CCSGG	1 cut(s)	18
Bsa29I	ATCGAT	2 cut(s)	833, 2932
BsaHI	GRCGYC	2 cut(s)	95, 1670
BsaI	GGTCTC	1 cut(s)	1991
BsaJI	CCNNGG	7 cut(s)	17, 237, 438, 956, 1235, 1434, 2154
BsaWI	WCCGGW	1 cut(s)	790
BsaXI	ACNNNNNCTCC	4 cut(s)	382, 412, 772, 802
Bsc4I	CCNNNNNNNGG	4 cut(s)	16, 944, 1198, 2171
Bse1I	ACTGG	7 cut(s)	599, 738, 888, 2223, 2740, 2758, 2956
Bse3DI	GCAATG	4 cut(s)	150, 975, 2191, 2823
BseBI	CCWGG	5 cut(s)	47, 636, 1236, 2463, 2595
BseCI	ATCGAT	2 cut(s)	833, 2932
BseDI	CCNNGG	7 cut(s)	17, 237, 438, 956, 1235, 1434, 2154
BseGI	GGATG	7 cut(s)	206, 226, 529, 693, 979, 1783, 2798
BseLI	CCNNNNNNNGG	4 cut(s)	16, 944, 1198, 2171
BseMI	GCAATG	4 cut(s)	150, 975, 2191, 2823
BseMII	CTCAG	5 cut(s)	95, 666, 1656, 2370, 2521
BseNI	ACTGG	7 cut(s)	599, 738, 888, 2223, 2740, 2758, 2956
BseRI	GAGGAG	3 cut(s)	14, 17, 1032
BseSI	GKGCMC	1 cut(s)	313
BseXI	GCAGC	6 cut(s)	64, 1259, 1315, 2312, 2824, 2934
Bsh1236I	CGCG	1 cut(s)	2283
BshFI	GGCC	3 cut(s)	5, 1213, 1234
BshNI	GGYRCC	2 cut(s)	200, 536
BshVI	ATCGAT	2 cut(s)	833, 2932
BsiHKAI	GWGCWC	3 cut(s)	1151, 1575, 2750
BsiHKCI	CYCGRG	2 cut(s)	549, 2622
BsiSI	CCGG	4 cut(s)	17, 218, 350, 791
BslFI	GGGAC	2 cut(s)	961, 2926
BslI	CCNNNNNNNGG	4 cut(s)	16, 944, 1198, 2171
BsmAI	GTCTC	3 cut(s)	254, 1991, 2619
BsmFI	GGGAC	2 cut(s)	961, 2926
BsmI	GAATGC	1 cut(s)	2140
BsnI	GGCC	3 cut(s)	5, 1213, 1234
Bso31I	GGTCTC	1 cut(s)	1991
BsoBI	CYCGRG	2 cut(s)	549, 2622
Bsp1286I	GDGCHC	5 cut(s)	24, 313, 1151, 1575, 2750
Bsp1407I	TGTACA	1 cut(s)	281
Bsp143I	GATC	9 cut(s)	830, 1430, 1516, 1621, 2024, 2449, 2490, 2929, 2933
Bsp1720I	GCTNAGC	1 cut(s)	2829
Bsp19I	CCATGG	1 cut(s)	956
BspACI	CCGC	6 cut(s)	10, 110, 113, 159, 720, 2281
BspANI	GGCC	3 cut(s)	5, 1213, 1234
BspCNI	CTCAG	5 cut(s)	94, 667, 1657, 2371, 2520
BspDI	ATCGAT	2 cut(s)	833, 2932
BspFNI	CGCG	1 cut(s)	2283
BspLI	GGNNCC	6 cut(s)	23, 202, 538, 1432, 1518, 2680
BspMI	ACCTGC	2 cut(s)	401, 1015
BspPI	GGATC	8 cut(s)	838, 1425, 1438, 1511, 1524, 1616, 2032, 2485
BspT107I	GGYRCC	2 cut(s)	200, 536
BspTI	CTTAAG	1 cut(s)	358
BspTNI	GGTCTC	1 cut(s)	1991
BsrDI	GCAATG	4 cut(s)	150, 975, 2191, 2823
BsrGI	TGTACA	1 cut(s)	281
BsrI	ACTGG	7 cut(s)	599, 738, 888, 2223, 2740, 2758, 2956
BssECI	CCNNGG	7 cut(s)	17, 237, 438, 956, 1235, 1434, 2154
BssMI	GATC	9 cut(s)	830, 1430, 1516, 1621, 2024, 2449, 2490, 2929, 2933
BssNI	GRCGYC	2 cut(s)	95, 1670
BssSI	CACGAG	1 cut(s)	2192
BssT1I	CCWWGG	4 cut(s)	237, 438, 956, 2154
Bst2BI	CACGAG	1 cut(s)	2192
Bst2UI	CCWGG	5 cut(s)	47, 636, 1236, 2463, 2595
Bst4CI	ACNGT	4 cut(s)	460, 769, 784, 1503
Bst6I	CTCTTC	1 cut(s)	1248
BstACI	GRCGYC	2 cut(s)	95, 1670
BstAFI	CTTAAG	1 cut(s)	358
BstAPI	GCANNNNNTGC	3 cut(s)	77, 1795, 2306
BstAUI	TGTACA	1 cut(s)	281
BstC8I	GCNNGC	8 cut(s)	161, 324, 355, 939, 1770, 2274, 2278, 2835
BstDEI	CTNAG	9 cut(s)	81, 675, 1665, 1709, 1971, 2239, 2379, 2507, 2829
BstDSI	CCRYGG	2 cut(s)	956, 1434
BstENI	CCTNNNNNAGG	1 cut(s)	2169
BstF5I	GGATG	7 cut(s)	206, 226, 529, 693, 979, 1783, 2798
BstFNI	CGCG	1 cut(s)	2283
BstHHI	GCGC	2 cut(s)	143, 1484
BstKTI	GATC	9 cut(s)	833, 1433, 1519, 1624, 2027, 2452, 2493, 2932, 2936
BstMAI	GTCTC	3 cut(s)	254, 1991, 2619
BstMBI	GATC	9 cut(s)	830, 1430, 1516, 1621, 2024, 2449, 2490, 2929, 2933
BstNI	CCWGG	5 cut(s)	47, 636, 1236, 2463, 2595
BstNSI	RCATGY	3 cut(s)	357, 1596, 2280
BstSCI	CCNGG	6 cut(s)	16, 45, 634, 1234, 2461, 2593
BstSFI	CTRYAG	6 cut(s)	432, 555, 765, 1161, 1291, 1499
BstSLI	GKGCMC	1 cut(s)	313
BstUI	CGCG	1 cut(s)	2283
BstV1I	GCAGC	6 cut(s)	64, 1259, 1315, 2312, 2824, 2934
BstX2I	RGATCY	3 cut(s)	1430, 1516, 2490
BstYI	RGATCY	3 cut(s)	1430, 1516, 2490
Bsu15I	ATCGAT	2 cut(s)	833, 2932
BsuI	GTATCC	1 cut(s)	1076
BsuRI	GGCC	3 cut(s)	5, 1213, 1234
BsuTUI	ATCGAT	2 cut(s)	833, 2932
BtgI	CCRYGG	2 cut(s)	956, 1434
BtsCI	GGATG	7 cut(s)	206, 226, 529, 693, 979, 1783, 2798
BtsI	GCAGTG	1 cut(s)	1746
BtsIMutI	CAGTG	7 cut(s)	731, 825, 881, 1746, 2047, 2216, 2765
BveI	ACCTGC	2 cut(s)	401, 1015
Cac8I	GCNNGC	8 cut(s)	161, 324, 355, 939, 1770, 2274, 2278, 2835
CaiI	CAGNNNCTG	1 cut(s)	944
CfoI	GCGC	2 cut(s)	143, 1484
Cfr13I	GGNCC	7 cut(s)	4, 241, 316, 1232, 1613, 2204, 2678
ClaI	ATCGAT	2 cut(s)	833, 2932
CseI	GACGC	1 cut(s)	1659
Csp6I	GTAC	6 cut(s)	181, 201, 282, 537, 1504, 1866
CviQI	GTAC	6 cut(s)	181, 201, 282, 537, 1504, 1866
DdeI	CTNAG	9 cut(s)	81, 675, 1665, 1709, 1971, 2239, 2379, 2507, 2829
DpnI	GATC	9 cut(s)	832, 1432, 1518, 1623, 2026, 2451, 2492, 2931, 2935
DpnII	GATC	9 cut(s)	830, 1430, 1516, 1621, 2024, 2449, 2490, 2929, 2933
DraI	TTTAAA	2 cut(s)	589, 1609
Eam1104I	CTCTTC	1 cut(s)	1248
EarI	CTCTTC	1 cut(s)	1248
Eco130I	CCWWGG	4 cut(s)	237, 438, 956, 2154
Eco147I	AGGCCT	1 cut(s)	1213
Eco24I	GRGCYC	1 cut(s)	24
Eco31I	GGTCTC	1 cut(s)	1991
Eco32I	GATATC	1 cut(s)	2871
Eco47I	GGWCC	5 cut(s)	241, 316, 1613, 2204, 2678
Eco57I	CTGAAG	2 cut(s)	744, 2621
Eco88I	CYCGRG	2 cut(s)	549, 2622
EcoNI	CCTNNNNNAGG	1 cut(s)	2169
EcoO109I	RGGNCCY	2 cut(s)	1232, 1613
EcoRI	GAATTC	2 cut(s)	544, 2586
EcoRII	CCWGG	5 cut(s)	45, 634, 1234, 2461, 2593
EcoRV	GATATC	1 cut(s)	2871
EcoT14I	CCWWGG	4 cut(s)	237, 438, 956, 2154
EcoT22I	ATGCAT	1 cut(s)	2411
EcoT38I	GRGCYC	1 cut(s)	24
ErhI	CCWWGG	4 cut(s)	237, 438, 956, 2154
FalI	AAGNNNNNCTT	4 cut(s)	1602, 1634, 1836, 1868
FaqI	GGGAC	2 cut(s)	961, 2926
FauI	CCCGC	2 cut(s)	106, 152
FauNDI	CATATG	3 cut(s)	73, 646, 2425
FbaI	TGATCA	1 cut(s)	2449
Fnu4HI	GCNGC	8 cut(s)	78, 111, 1248, 1304, 2281, 2301, 2838, 2948
FokI	GGATG	7 cut(s)	193, 233, 536, 700, 986, 1790, 2785
FriOI	GRGCYC	1 cut(s)	24
Fsp4HI	GCNGC	8 cut(s)	78, 111, 1248, 1304, 2281, 2301, 2838, 2948
FspBI	CTAG	9 cut(s)	924, 1125, 1280, 1350, 1626, 1803, 2099, 2223, 2565
FspI	TGCGCA	1 cut(s)	142
GlaI	GCGC	2 cut(s)	142, 1483
GluI	GCNGC	8 cut(s)	78, 111, 1248, 1304, 2281, 2301, 2838, 2948
GsuI	CTGGAG	2 cut(s)	2859, 2910
HaeIII	GGCC	3 cut(s)	5, 1213, 1234
HapII	CCGG	4 cut(s)	17, 218, 350, 791
HgaI	GACGC	1 cut(s)	1659
HhaI	GCGC	2 cut(s)	143, 1484
Hin1I	GRCGYC	2 cut(s)	95, 1670
Hin6I	GCGC	2 cut(s)	141, 1482
HinP1I	GCGC	2 cut(s)	141, 1482
HincII	GTYRAC	1 cut(s)	445
HindII	GTYRAC	1 cut(s)	445
HpaI	GTTAAC	1 cut(s)	445
HpaII	CCGG	4 cut(s)	17, 218, 350, 791
HphI	GGTGA	7 cut(s)	509, 734, 761, 1338, 1932, 1946, 2143
Hpy166II	GTNNAC	3 cut(s)	445, 1699, 2904
Hpy8I	GTNNAC	3 cut(s)	445, 1699, 2904
HpyAV	CCTTC	8 cut(s)	125, 494, 672, 768, 1393, 2165, 2339, 2479
HpyCH4III	ACNGT	4 cut(s)	460, 769, 784, 1503
HpyCH4IV	ACGT	3 cut(s)	95, 166, 1047
HpyF3I	CTNAG	9 cut(s)	81, 675, 1665, 1709, 1971, 2239, 2379, 2507, 2829
HpySE526I	ACGT	3 cut(s)	95, 166, 1047
Hsp92I	GRCGYC	2 cut(s)	95, 1670
HspAI	GCGC	2 cut(s)	141, 1482
KpnI	GGTACC	2 cut(s)	204, 540
Ksp22I	TGATCA	1 cut(s)	2449
KspAI	GTTAAC	1 cut(s)	445
Kzo9I	GATC	9 cut(s)	830, 1430, 1516, 1621, 2024, 2449, 2490, 2929, 2933
LmnI	GCTCC	3 cut(s)	27, 1045, 2503
Lsp1109I	GCAGC	6 cut(s)	64, 1259, 1315, 2312, 2824, 2934
LweI	GCATC	4 cut(s)	1011, 1027, 1864, 2807
MaeI	CTAG	9 cut(s)	924, 1125, 1280, 1350, 1626, 1803, 2099, 2223, 2565
MaeII	ACGT	3 cut(s)	95, 166, 1047
MalI	GATC	9 cut(s)	832, 1432, 1518, 1623, 2026, 2451, 2492, 2931, 2935
MboI	GATC	9 cut(s)	830, 1430, 1516, 1621, 2024, 2449, 2490, 2929, 2933
MfeI	CAATTG	1 cut(s)	1071
MflI	RGATCY	3 cut(s)	1430, 1516, 2490
MhlI	GDGCHC	5 cut(s)	24, 313, 1151, 1575, 2750
MlyI	GAGTC	4 cut(s)	686, 2467, 2529, 2621
MmeI	TCCRAC	5 cut(s)	281, 1087, 2182, 2233, 2876
Mph1103I	ATGCAT	1 cut(s)	2411
MroXI	GAANNNNTTC	2 cut(s)	2082, 2705
MslI	CAYNNNNRTG	2 cut(s)	1595, 2887
MspCI	CTTAAG	1 cut(s)	358
MspI	CCGG	4 cut(s)	17, 218, 350, 791
MspR9I	CCNGG	6 cut(s)	18, 47, 636, 1236, 2463, 2595
MunI	CAATTG	1 cut(s)	1071
Mva1269I	GAATGC	1 cut(s)	2140
MvaI	CCWGG	5 cut(s)	47, 636, 1236, 2463, 2595
MvnI	CGCG	1 cut(s)	2283
NciI	CCSGG	1 cut(s)	18
NcoI	CCATGG	1 cut(s)	956
NdeI	CATATG	3 cut(s)	73, 646, 2425
NdeII	GATC	9 cut(s)	830, 1430, 1516, 1621, 2024, 2449, 2490, 2929, 2933
NlaIV	GGNNCC	6 cut(s)	23, 202, 538, 1432, 1518, 2680
NmuCI	GTSAC	3 cut(s)	91, 424, 1357
NsbI	TGCGCA	1 cut(s)	142
NsiI	ATGCAT	1 cut(s)	2411
NspI	RCATGY	3 cut(s)	357, 1596, 2280
PaeI	GCATGC	2 cut(s)	357, 2280
PaeR7I	CTCGAG	1 cut(s)	549
PceI	AGGCCT	1 cut(s)	1213
PciI	ACATGT	1 cut(s)	1592
PctI	GAATGC	1 cut(s)	2140
PdmI	GAANNNNTTC	2 cut(s)	2082, 2705
PfeI	GAWTC	9 cut(s)	328, 657, 1129, 1937, 2458, 2723, 2765, 2771, 2813
PflMI	CCANNNNNTGG	1 cut(s)	944
PkrI	GCNGC	8 cut(s)	79, 112, 1249, 1305, 2282, 2302, 2839, 2949
PleI	GAGTC	4 cut(s)	685, 2467, 2528, 2621
PpsI	GAGTC	4 cut(s)	685, 2467, 2528, 2621
PpuMI	RGGWCCY	1 cut(s)	1613
PscI	ACATGT	1 cut(s)	1592
Psp5II	RGGWCCY	1 cut(s)	1613
Psp6I	CCWGG	5 cut(s)	45, 634, 1234, 2461, 2593
PspGI	CCWGG	5 cut(s)	45, 634, 1234, 2461, 2593
PspN4I	GGNNCC	6 cut(s)	23, 202, 538, 1432, 1518, 2680
PspPI	GGNCC	7 cut(s)	4, 241, 316, 1232, 1613, 2204, 2678
PspPPI	RGGWCCY	1 cut(s)	1613
PstNI	CAGNNNCTG	1 cut(s)	944
PsuI	RGATCY	3 cut(s)	1430, 1516, 2490
RsaI	GTAC	6 cut(s)	182, 202, 283, 538, 1505, 1867
RsaNI	GTAC	6 cut(s)	181, 201, 282, 537, 1504, 1866
RseI	CAYNNNNRTG	2 cut(s)	1595, 2887
SatI	GCNGC	8 cut(s)	78, 111, 1248, 1304, 2281, 2301, 2838, 2948
Sau3AI	GATC	9 cut(s)	830, 1430, 1516, 1621, 2024, 2449, 2490, 2929, 2933
Sau96I	GGNCC	7 cut(s)	4, 241, 316, 1232, 1613, 2204, 2678
ScaI	AGTACT	1 cut(s)	1505
SchI	GAGTC	4 cut(s)	686, 2467, 2529, 2621
ScrFI	CCNGG	6 cut(s)	18, 47, 636, 1236, 2463, 2595
SduI	GDGCHC	5 cut(s)	24, 313, 1151, 1575, 2750
SfaNI	GCATC	4 cut(s)	1011, 1027, 1864, 2807
SfcI	CTRYAG	6 cut(s)	432, 555, 765, 1161, 1291, 1499
Sfr274I	CTCGAG	1 cut(s)	549
SinI	GGWCC	5 cut(s)	241, 316, 1613, 2204, 2678
SlaI	CTCGAG	1 cut(s)	549
SmiMI	CAYNNNNRTG	2 cut(s)	1595, 2887
SmlI	CTYRAG	2 cut(s)	358, 549
SmoI	CTYRAG	2 cut(s)	358, 549
SphI	GCATGC	2 cut(s)	357, 2280
SseBI	AGGCCT	1 cut(s)	1213
SsiI	CCGC	6 cut(s)	10, 110, 113, 159, 720, 2281
SspI	AATATT	3 cut(s)	1311, 1372, 1887
SspMI	CTAG	9 cut(s)	924, 1125, 1280, 1350, 1626, 1803, 2099, 2223, 2565
StuI	AGGCCT	1 cut(s)	1213
StyD4I	CCNGG	6 cut(s)	16, 45, 634, 1234, 2461, 2593
StyI	CCWWGG	4 cut(s)	237, 438, 956, 2154
TaaI	ACNGT	4 cut(s)	460, 769, 784, 1503
TaiI	ACGT	3 cut(s)	98, 169, 1050
TaqI	TCGA	4 cut(s)	462, 550, 833, 2932
TatI	WGTACW	4 cut(s)	180, 281, 1503, 1865
TauI	GCSGC	2 cut(s)	113, 2283
TfiI	GAWTC	9 cut(s)	328, 657, 1129, 1937, 2458, 2723, 2765, 2771, 2813
TscAI	CASTG	7 cut(s)	738, 825, 888, 1746, 2047, 2223, 2765
TseFI	GTSAC	3 cut(s)	91, 424, 1357
TseI	GCWGC	6 cut(s)	77, 1247, 1303, 2300, 2837, 2947
Tsp45I	GTSAC	3 cut(s)	91, 424, 1357
TspGWI	ACGGA	4 cut(s)	79, 199, 1181, 1423
TspRI	CASTG	7 cut(s)	738, 825, 888, 1746, 2047, 2223, 2765
Van91I	CCANNNNNTGG	1 cut(s)	944
Vha464I	CTTAAG	1 cut(s)	358
VpaK11BI	GGWCC	5 cut(s)	241, 316, 1613, 2204, 2678
XagI	CCTNNNNNAGG	1 cut(s)	2169
XapI	RAATTY	7 cut(s)	373, 544, 560, 583, 1094, 2415, 2586
XbaI	TCTAGA	1 cut(s)	1802
XceI	RCATGY	3 cut(s)	357, 1596, 2280
XhoI	CTCGAG	1 cut(s)	549
XmnI	GAANNNNTTC	2 cut(s)	2082, 2705
XspI	CTAG	9 cut(s)	924, 1125, 1280, 1350, 1626, 1803, 2099, 2223, 2565
ZraI	GACGTC	1 cut(s)	96
ZrmI	AGTACT	1 cut(s)	1505
Zsp2I	ATGCAT	1 cut(s)	2411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.