pycom06g01270

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012348
Physical Location & Seq
Forward (+)
1323239 .. 1324409
1171 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 720 bp
ATGGCGAGCGAAAATGTTCACGCAGCTGCAAGGTCCGGTAATCTTCCTTCACTGCAGTCAATTCTCGCCTCCAACCCTCTCGCCGTCAATTCCAGAGACAAGCACTCCAGAACTCCACTACATTTAGCGGCATGGTCTGGCCAAACGGAGGTGGTGAATTTTCTCTGTAGCCACAAGGCGGATGTTGGTGCTGCTGCTATGGACGATATGGGTGCTATACACTTTGCTGCCCAAAAGGGACATTTAGAGGTCGTTCGCACTCTGCTTTCATCTGGGGCTTCTATAAAAGCTTCCACTCGCAAAGGCTTCACTCCGCTCCACTATGCTGTTCAAGGGTCTCATGTCGAGCTTATCAAGTATCTGGTAAAGAAAGGTGCCAATCTGAGTGTGAAGACAAAAGCTGGGAAGACCCCTCTTGATCTTGCTAACAGCGATGATGTCCGCTCTTGTTTAGAAGGGTGTGAAAGGTCATCCAAGACAGAAGATATGGATGGTAAACAGAAAGATAAAGAATCTGATCCAAAGACATCCGTGCAGGAAGAAGTGAAACCGGAAGGTGAAGCTGCAACTTCTGGGAACGATAAACAGGCCGAGGATGAAAGTCTGAAGCGGAAGGGAGACAAAAATGACGCTGAGGAAGCCGCAGGGGAACGTAAAAAGGCCAAAGTTGCATTGAATCATCTTCTAACTGCCGATGATATGCAAGACGAGGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5

Protein Analysis

240

Amino Acids

25.63

Weight (kDa)

6.01

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_4 PF13637 6 - 55 1.5e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 7 - 63 7.1e-09 Ankyrin repeats (3 copies)
Ank PF00023 37 - 64 1.9e-06 Ankyrin repeat
Ank_2 PF12796 73 - 142 1.8e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 73 - 122 1e-13 Ankyrin repeats (many copies)
Ank_5 PF13857 93 - 142 1.4e-09 Ankyrin repeats (many copies)
Ank PF00023 102 - 133 2.9e-08 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012348)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19150 AT4G19150
fragaria_vesca FvH4_5g24000
prunus_persica Prupe.5G019900_v2.0.a1
pyrus_communis pycom06g01270
rosa_chinensis RchiOBHm_Chr7g0213461
rosa_laevigata RLG00000002802 RLG00000007782
rosa_multiflora Rmu_co8493477.1_g000002 Rmu_sc0004455.1_g000006 Rmu_sc0013012.1_g000011
rosa_roxburghii Rroxscaffold_3G00245860
rosa_rugosa Rorug07G0142400
rosa_samantha Rh4BG226300 Rh7AG274500 Rh7BG270000 Rh7CG293700 Rh7DG282000
rosa_wichuraiana Rw7G023810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 374
AccBSI CCGCTC 2 cut(s) 316, 444
AciI CCGC 6 cut(s) 128, 179, 314, 442, 610, 642
AclWI GGATC 1 cut(s) 512
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 1 cut(s) 157
AcuI CTGAAG 1 cut(s) 626
AfiI CCNNNNNNNGG 2 cut(s) 148, 178
AgsI TTSAA 2 cut(s) 332, 676
AluBI AGCT 5 cut(s) 26, 290, 349, 401, 563
AluI AGCT 5 cut(s) 26, 290, 349, 401, 563
Alw26I GTCTC 3 cut(s) 90, 342, 612
AlwI GGATC 1 cut(s) 512
AoxI GGCC 3 cut(s) 139, 588, 660
ApeKI GCWGC 6 cut(s) 23, 26, 191, 194, 227, 563
ApoI RAATTY 1 cut(s) 157
Asp700I GAANNNNTTC 1 cut(s) 15
AspS9I GGNCC 1 cut(s) 33
AsuHPI GGTGA 2 cut(s) 166, 569
AvaII GGWCC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 141
BanI GGYRCC 1 cut(s) 374
BarI GAAGNNNNNNTAC 2 cut(s) 31, 63
BbsI GAAGAC 2 cut(s) 398, 413
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 6 cut(s) 13, 35, 178, 181, 214, 550
BccI CCATC 1 cut(s) 485
BceAI ACGGC 1 cut(s) 68
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BcoDI GTCTC 3 cut(s) 90, 342, 612
BfmI CTRYAG 2 cut(s) 53, 166
BisI GCNGC 8 cut(s) 24, 27, 129, 192, 195, 228, 564, 642
BlsI GCNGC 8 cut(s) 25, 28, 130, 193, 196, 229, 565, 643
Bme18I GGWCC 1 cut(s) 33
BmgT120I GGNCC 1 cut(s) 33
BmiI GGNNCC 1 cut(s) 376
BpiI GAAGAC 2 cut(s) 398, 413
BpmI CTGGAG 1 cut(s) 91
Bpu10I CCTNAGC 1 cut(s) 633
BsaI GGTCTC 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 591
BsaWI WCCGGW 2 cut(s) 35, 550
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 178
BseDI CCNNGG 1 cut(s) 591
BseGI GGATG 5 cut(s) 187, 470, 496, 527, 601
BseLI CCNNNNNNNGG 2 cut(s) 148, 178
BseMII CTCAG 2 cut(s) 374, 624
BseXI GCAGC 6 cut(s) 13, 35, 178, 181, 214, 550
BseYI CCCAGC 1 cut(s) 401
BsgI GTGCAG 1 cut(s) 554
BshFI GGCC 3 cut(s) 141, 590, 662
BshNI GGYRCC 1 cut(s) 374
BsiSI CCGG 2 cut(s) 36, 551
BslFI GGGAC 1 cut(s) 252
BslI CCNNNNNNNGG 2 cut(s) 148, 178
BsmAI GTCTC 3 cut(s) 90, 342, 612
BsmFI GGGAC 1 cut(s) 252
BsnI GGCC 3 cut(s) 141, 590, 662
Bso31I GGTCTC 1 cut(s) 342
Bsp143I GATC 2 cut(s) 418, 517
BspACI CCGC 6 cut(s) 128, 179, 314, 442, 610, 642
BspANI GGCC 3 cut(s) 141, 590, 662
BspCNI CTCAG 2 cut(s) 375, 625
BspLI GGNNCC 1 cut(s) 376
BspMAI CTGCAG 1 cut(s) 57
BspPI GGATC 1 cut(s) 512
BspT107I GGYRCC 1 cut(s) 374
BspTNI GGTCTC 1 cut(s) 342
BsrBI CCGCTC 2 cut(s) 316, 444
BssECI CCNNGG 1 cut(s) 591
BssMI GATC 2 cut(s) 418, 517
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 2 cut(s) 383, 633
BstF5I GGATG 5 cut(s) 187, 470, 496, 527, 601
BstKTI GATC 2 cut(s) 421, 520
BstMAI GTCTC 3 cut(s) 90, 342, 612
BstMBI GATC 2 cut(s) 418, 517
BstMWI GCNNNNNNNGC 2 cut(s) 638, 668
BstSFI CTRYAG 2 cut(s) 53, 166
BstV1I GCAGC 6 cut(s) 13, 35, 178, 181, 214, 550
BstV2I GAAGAC 2 cut(s) 398, 413
BsuRI GGCC 3 cut(s) 141, 590, 662
BtgZI GCGATG 1 cut(s) 447
BtsCI GGATG 5 cut(s) 187, 470, 496, 527, 601
BtsI GCAGTG 1 cut(s) 50
BtsIMutI CAGTG 1 cut(s) 50
Cac8I GCNNGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 33
CseI GACGC 1 cut(s) 638
CviAII CATG 2 cut(s) 132, 341
DdeI CTNAG 2 cut(s) 383, 633
DpnI GATC 2 cut(s) 420, 519
DpnII GATC 2 cut(s) 418, 517
EaeI YGGCCR 1 cut(s) 139
EciI GGCGGA 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 342
Eco47I GGWCC 1 cut(s) 33
Eco57I CTGAAG 1 cut(s) 626
FaeI CATG 2 cut(s) 135, 344
FaiI YATR 9 cut(s) 133, 200, 209, 218, 284, 324, 342, 488, 701
FaqI GGGAC 1 cut(s) 252
FatI CATG 2 cut(s) 131, 340
Fnu4HI GCNGC 8 cut(s) 24, 27, 129, 192, 195, 228, 564, 642
FokI GGATG 5 cut(s) 194, 457, 503, 514, 608
Fsp4HI GCNGC 8 cut(s) 24, 27, 129, 192, 195, 228, 564, 642
GluI GCNGC 8 cut(s) 24, 27, 129, 192, 195, 228, 564, 642
GsaI CCCAGC 1 cut(s) 405
GsuI CTGGAG 1 cut(s) 91
HaeIII GGCC 3 cut(s) 141, 590, 662
HapII CCGG 2 cut(s) 36, 551
HgaI GACGC 1 cut(s) 638
Hin1II CATG 2 cut(s) 135, 344
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 2 cut(s) 512, 676
HpaII CCGG 2 cut(s) 36, 551
HphI GGTGA 2 cut(s) 166, 569
Hpy166II GTNNAC 2 cut(s) 19, 497
Hpy188I TCNGA 3 cut(s) 384, 517, 606
Hpy188III TCNNGA 3 cut(s) 93, 108, 416
Hpy8I GTNNAC 2 cut(s) 19, 497
HpyAV CCTTC 4 cut(s) 57, 449, 548, 607
HpyCH4IV ACGT 1 cut(s) 652
HpyCH4V TGCA 6 cut(s) 29, 55, 535, 566, 671, 703
HpyF10VI GCNNNNNNNGC 2 cut(s) 638, 668
HpyF3I CTNAG 2 cut(s) 383, 633
HpySE526I ACGT 1 cut(s) 652
Hsp92II CATG 2 cut(s) 135, 344
Kzo9I GATC 2 cut(s) 418, 517
LmnI GCTCC 1 cut(s) 321
Lsp1109I GCAGC 6 cut(s) 13, 35, 178, 181, 214, 550
MaeII ACGT 1 cut(s) 652
MalI GATC 2 cut(s) 420, 519
MbiI CCGCTC 2 cut(s) 316, 444
MboI GATC 2 cut(s) 418, 517
MboII GAAGA 6 cut(s) 35, 403, 418, 494, 551, 674
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 3 cut(s) 60, 88, 157
MluNI TGGCCA 1 cut(s) 141
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 9 cut(s) 79, 87, 142, 241, 423, 586, 628, 703, 706
Mox20I TGGCCA 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 15
MscI TGGCCA 1 cut(s) 141
Msp20I TGGCCA 1 cut(s) 141
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 2 cut(s) 36, 551
MwoI GCNNNNNNNGC 2 cut(s) 638, 668
NdeII GATC 2 cut(s) 418, 517
NlaIII CATG 2 cut(s) 135, 344
NlaIV GGNNCC 1 cut(s) 376
NmeAIII GCCGAG 1 cut(s) 616
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 2 cut(s) 512, 676
PkrI GCNGC 8 cut(s) 25, 28, 130, 193, 196, 229, 565, 643
PspFI CCCAGC 1 cut(s) 401
PspN4I GGNNCC 1 cut(s) 376
PspPI GGNCC 1 cut(s) 33
PstI CTGCAG 1 cut(s) 57
PvuII CAGCTG 1 cut(s) 26
SatI GCNGC 8 cut(s) 24, 27, 129, 192, 195, 228, 564, 642
Sau3AI GATC 2 cut(s) 418, 517
Sau96I GGNCC 1 cut(s) 33
SfcI CTRYAG 2 cut(s) 53, 166
SinI GGWCC 1 cut(s) 33
Sse9I AATT 3 cut(s) 60, 88, 157
SsiI CCGC 6 cut(s) 128, 179, 314, 442, 610, 642
TaiI ACGT 1 cut(s) 655
TaqI TCGA 1 cut(s) 345
TasI AATT 3 cut(s) 60, 88, 157
TauI GCSGC 2 cut(s) 131, 644
TfiI GAWTC 2 cut(s) 512, 676
TscAI CASTG 1 cut(s) 57
TseI GCWGC 6 cut(s) 23, 26, 191, 194, 227, 563
TspDTI ATGAA 2 cut(s) 258, 612
TspGWI ACGGA 2 cut(s) 161, 520
TspRI CASTG 1 cut(s) 57
VpaK11BI GGWCC 1 cut(s) 33
XapI RAATTY 1 cut(s) 157
XmnI GAANNNNTTC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.