pycom06g06450

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012060
Physical Location & Seq
Reverse (-)
10160413 .. 10162786
2374 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1278 bp
ATGGAGTGGACTGCTTGCCTCGGCGAATATGAGAAGCTCGTCTTCCGGATGAACACCCCCAGGGTCGTGATCGACAATGCCGTCTGCTCCACTGCGACTTTAGTCAAGGTCCTCACGGATCTCAACCTCTCCATTAAAAAAGCTTACATTTCTTCTGATGGTCGCTGGTTCATGGCTGTTTTTCATGTAACTGATCAGAACGGGAATAAATTAACAGACGAGAGCGTTATTAACTATATTGAACAGTCACTTGAGACAATTCATTTTGGTACATCCAACTGTAGTAACGGTCTTACGGCCCTTGAGCTCACCGGGACAGACCGAGTAGGCCTTCTTTCCGAGGTGTTTGCGGTGCTAGCTGACCTGCAATGCATCGTGGTTGAGTCCAAGGTGTGGACGCACAATGGCCGAATTGCTTCATTGATTTATGTTAAGGATTGTGATTCCGGTTGCCCGATTGAGGACTCACAGAAAATTGATAGGATTGAAGCGCGGTTAAGGAATGTTCTTAAAGGGGACAATGATATTCGAAGCGCGAAAACCTCAGTTTCAATGGCAGTTACACACACCGAAAGAAGACTGCATCAAATGATGTTTGCAGATCGCGATTATGACAGAAAGCCCGTTTTACAGCATTGCACCAATTCTCCGGTCATAACGGTGCAGAATTGGGCTGAAAGGGGTTATTCCATAGTGAATATTCAGTGCAAGGATAGGGCAAAGCTTTTGTTCGACGTTGTTTGCACGTTGACAGACATGGACTACGAGGTCTTTCATGCCACTATTAATACATCTGGGGACAGAGGGTACATGGAATTTTACATTAGGCACACTGATGGAACCCCAATTAGCTCCGAACCCGAACAACAGCGTGTAATCCAATGCTTACAGGCCGCGGTTCAAAGAAGAGCATCTGAGGGTGTGAGGCTAGAACTATGCACCGAGGATAGACAAGGTCTTCTAGCTTATGTTACAAGGACATTCCGCGAGAACGGTCTAAACGTGACGAGGGCCGAGATATCCACGACAACGAACAAGGCGCTAAACGTATTCTACGTGACGGATGCAATGGGAAACCTTGCGGATGCGAAAACCATAGAATCAGTGAGACAGAAAATCGGGTTGAGTAACTTGAAAGTGAAGGAACTTCCATTGATTTACCATCAAAGGAGGGAGAGGGAGGAGCAGCAAGGAGTTGGTGGGGCAGTTTTGTTATCACTTGGGAGCCTAGTGAGAAGGAATCTGTACAATTTGGGGCTCATCAGATCTCATTATTAA

Protein Analysis

426

Amino Acids

47.97

Weight (kDa)

6.57

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT_ACR9_3rd PF24931 219 - 301 5.4e-09 ACR9-like, ACT-like domain
ACT PF01842 231 - 281 3.6e-07 ACT domain
ACT PF01842 309 - 369 1.4e-07 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12420 AT1G12420
fragaria_vesca FvH4_5g00870 FvH4_5g00870
malus_domestica MD00G1083000.v1.1 MD00G1168800.v1.1
prunus_persica Prupe.5G081600_v2.0.a1
pyrus_communis pycom04g06870 pycom06g06450
rosa_chinensis RchiOBHm_Chr7g0201021
rosa_laevigata RLG00000003697
rosa_multiflora Rmu_sc0006553.1_g000010
rosa_roxburghii Rroxscaffold_3G00255590
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187200 Rh7BG190700 Rh7CG198200 Rh7DG192800
rosa_wichuraiana Rw7G016440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 80, 767
Acc36I ACCTGC 1 cut(s) 372
AccB7I CCANNNNNTGG 1 cut(s) 393
AccII CGCG 5 cut(s) 493, 536, 606, 896, 987
AccIII TCCGGA 1 cut(s) 45
AciI CCGC 6 cut(s) 350, 493, 894, 896, 985, 1082
AclWI GGATC 1 cut(s) 126
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 1 cut(s) 815
AfaI GTAC 3 cut(s) 271, 809, 1247
AfiI CCNNNNNNNGG 2 cut(s) 393, 460
AgsI TTSAA 5 cut(s) 242, 488, 552, 902, 1135
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 7 cut(s) 37, 143, 307, 359, 724, 852, 965
AluI AGCT 7 cut(s) 37, 143, 307, 359, 724, 852, 965
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 2 cut(s) 248, 1102
AlwI GGATC 1 cut(s) 126
Aor13HI TCCGGA 1 cut(s) 45
AoxI GGCC 5 cut(s) 297, 328, 406, 891, 1011
ApeKI GCWGC 1 cut(s) 1186
ApoI RAATTY 1 cut(s) 815
AseI ATTAAT 1 cut(s) 786
Asp700I GAANNNNTTC 1 cut(s) 415
AspLEI GCGC 3 cut(s) 493, 536, 1042
AspS9I GGNCC 3 cut(s) 109, 298, 1011
AsuC2I CCSGG 1 cut(s) 313
AsuHPI GGTGA 1 cut(s) 301
AsuII TTCGAA 1 cut(s) 529
AsuNHI GCTAGC 1 cut(s) 355
AvaII GGWCC 1 cut(s) 109
BanII GRGCYC 2 cut(s) 309, 1260
BbsI GAAGAC 3 cut(s) 34, 583, 950
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 1 cut(s) 1198
BccI CCATC 3 cut(s) 152, 830, 1170
BceAI ACGGC 2 cut(s) 65, 312
BcgI CGANNNNNNTGC 2 cut(s) 329, 363
BciT130I CCWGG 1 cut(s) 61
BclI TGATCA 1 cut(s) 193
BcnI CCSGG 1 cut(s) 313
BcoDI GTCTC 2 cut(s) 248, 1102
BfaI CTAG 4 cut(s) 356, 929, 962, 1229
BfmI CTRYAG 1 cut(s) 280
BfoI RGCGCY 1 cut(s) 1043
BfuAI ACCTGC 1 cut(s) 372
BglII AGATCT 1 cut(s) 1265
BisI GCNGC 2 cut(s) 894, 1187
BlsI GCNGC 2 cut(s) 895, 1188
Bme1390I CCNGG 2 cut(s) 61, 313
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 3 cut(s) 109, 298, 1011
BmiI GGNNCC 2 cut(s) 841, 1226
BmrFI CCNGG 2 cut(s) 61, 313
BmsI GCATC 5 cut(s) 381, 592, 920, 1054, 1075
BmtI GCTAGC 1 cut(s) 359
BoxI GACNNNNGTC 1 cut(s) 101
BpiI GAAGAC 3 cut(s) 34, 583, 950
Bpu14I TTCGAA 1 cut(s) 529
BpuEI CTTGAG 2 cut(s) 272, 323
BpuMI CCSGG 1 cut(s) 313
BsaAI YACGTR 1 cut(s) 1057
BsaJI CCNNGG 7 cut(s) 19, 59, 60, 339, 387, 894, 942
BsaWI WCCGGW 3 cut(s) 45, 446, 649
BsaXI ACNNNNNCTCC 2 cut(s) 631, 661
Bsc4I CCNNNNNNNGG 2 cut(s) 393, 460
Bse3DI GCAATG 3 cut(s) 374, 634, 1074
BseAI TCCGGA 1 cut(s) 45
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 7 cut(s) 19, 59, 60, 339, 387, 894, 942
BseGI GGATG 4 cut(s) 54, 272, 1069, 1090
BseLI CCNNNNNNNGG 2 cut(s) 393, 460
BseMI GCAATG 3 cut(s) 374, 634, 1074
BseMII CTCAG 2 cut(s) 558, 906
BseRI GAGGAG 1 cut(s) 1196
BseXI GCAGC 1 cut(s) 1198
BsgI GTGCAG 1 cut(s) 683
Bsh1236I CGCG 5 cut(s) 493, 536, 606, 896, 987
BshFI GGCC 5 cut(s) 299, 330, 408, 893, 1013
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 4 cut(s) 46, 312, 447, 650
BslFI GGGAC 3 cut(s) 328, 530, 812
BslI CCNNNNNNNGG 2 cut(s) 393, 460
BsmAI GTCTC 2 cut(s) 248, 1102
BsmFI GGGAC 3 cut(s) 328, 530, 812
BsnI GGCC 5 cut(s) 299, 330, 408, 893, 1013
Bsp119I TTCGAA 1 cut(s) 529
Bsp1286I GDGCHC 2 cut(s) 309, 1260
Bsp13I TCCGGA 1 cut(s) 45
Bsp1407I TGTACA 1 cut(s) 1245
Bsp143I GATC 5 cut(s) 69, 118, 193, 601, 1265
Bsp68I TCGCGA 1 cut(s) 606
BspACI CCGC 6 cut(s) 350, 493, 894, 896, 985, 1082
BspANI GGCC 5 cut(s) 299, 330, 408, 893, 1013
BspCNI CTCAG 2 cut(s) 557, 907
BspEI TCCGGA 1 cut(s) 45
BspFNI CGCG 5 cut(s) 493, 536, 606, 896, 987
BspLI GGNNCC 2 cut(s) 841, 1226
BspMI ACCTGC 1 cut(s) 372
BspOI GCTAGC 1 cut(s) 359
BspPI GGATC 1 cut(s) 126
BspQI GCTCTTC 1 cut(s) 901
BspT104I TTCGAA 1 cut(s) 529
BsrDI GCAATG 3 cut(s) 374, 634, 1074
BsrGI TGTACA 1 cut(s) 1245
BssECI CCNNGG 7 cut(s) 19, 59, 60, 339, 387, 894, 942
BssMI GATC 5 cut(s) 69, 118, 193, 601, 1265
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 5 cut(s) 246, 281, 290, 661, 995
Bst6I CTCTTC 1 cut(s) 901
BstAUI TGTACA 1 cut(s) 1245
BstBAI YACGTR 1 cut(s) 1057
BstBI TTCGAA 1 cut(s) 529
BstC8I GCNNGC 2 cut(s) 16, 357
BstDEI CTNAG 2 cut(s) 544, 915
BstDSI CCRYGG 1 cut(s) 894
BstF5I GGATG 4 cut(s) 54, 272, 1069, 1090
BstFNI CGCG 5 cut(s) 493, 536, 606, 896, 987
BstH2I RGCGCY 1 cut(s) 1043
BstHHI GCGC 3 cut(s) 493, 536, 1042
BstKTI GATC 5 cut(s) 72, 121, 196, 604, 1268
BstMAI GTCTC 2 cut(s) 248, 1102
BstMBI GATC 5 cut(s) 69, 118, 193, 601, 1265
BstMWI GCNNNNNNNGC 1 cut(s) 356
BstNI CCWGG 1 cut(s) 61
BstPAI GACNNNNGTC 1 cut(s) 101
BstSCI CCNGG 2 cut(s) 59, 311
BstSFI CTRYAG 1 cut(s) 280
BstUI CGCG 5 cut(s) 493, 536, 606, 896, 987
BstV1I GCAGC 1 cut(s) 1198
BstV2I GAAGAC 3 cut(s) 34, 583, 950
BstX2I RGATCY 2 cut(s) 118, 1265
BstYI RGATCY 2 cut(s) 118, 1265
BsuRI GGCC 5 cut(s) 299, 330, 408, 893, 1013
BtgI CCRYGG 1 cut(s) 894
BtsCI GGATG 4 cut(s) 54, 272, 1069, 1090
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 4 cut(s) 90, 710, 831, 1110
BtuMI TCGCGA 1 cut(s) 606
BveI ACCTGC 1 cut(s) 372
Cac8I GCNNGC 2 cut(s) 16, 357
CfoI GCGC 3 cut(s) 493, 536, 1042
Cfr13I GGNCC 3 cut(s) 109, 298, 1011
Cfr42I CCGCGG 1 cut(s) 897
CseI GACGC 1 cut(s) 406
Csp6I GTAC 3 cut(s) 270, 808, 1246
CviAII CATG 5 cut(s) 172, 185, 757, 776, 811
CviQI GTAC 3 cut(s) 270, 808, 1246
DdeI CTNAG 2 cut(s) 544, 915
DpnI GATC 5 cut(s) 71, 120, 195, 603, 1267
DpnII GATC 5 cut(s) 69, 118, 193, 601, 1265
DrdI GACNNNNNNGTC 2 cut(s) 80, 767
DseDI GACNNNNNNGTC 2 cut(s) 80, 767
EaeI YGGCCR 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 901
EarI CTCTTC 1 cut(s) 901
Ecl136II GAGCTC 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 387
Eco147I AGGCCT 1 cut(s) 330
Eco24I GRGCYC 2 cut(s) 309, 1260
Eco32I GATATC 1 cut(s) 1020
Eco47I GGWCC 1 cut(s) 109
Eco53kI GAGCTC 1 cut(s) 307
EcoICRI GAGCTC 1 cut(s) 307
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 59
EcoRV GATATC 1 cut(s) 1020
EcoT14I CCWWGG 1 cut(s) 387
EcoT22I ATGCAT 1 cut(s) 374
EcoT38I GRGCYC 2 cut(s) 309, 1260
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 5 cut(s) 175, 188, 760, 779, 814
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 3 cut(s) 328, 530, 812
FatI CATG 5 cut(s) 171, 184, 756, 775, 810
FbaI TGATCA 1 cut(s) 193
Fnu4HI GCNGC 2 cut(s) 894, 1187
FokI GGATG 4 cut(s) 61, 259, 1076, 1097
FriOI GRGCYC 2 cut(s) 309, 1260
Fsp4HI GCNGC 2 cut(s) 894, 1187
FspBI CTAG 4 cut(s) 356, 929, 962, 1229
GlaI GCGC 3 cut(s) 492, 535, 1041
GluI GCNGC 2 cut(s) 894, 1187
HaeII RGCGCY 1 cut(s) 1043
HaeIII GGCC 5 cut(s) 299, 330, 408, 893, 1013
HapII CCGG 4 cut(s) 46, 312, 447, 650
HgaI GACGC 1 cut(s) 406
HhaI GCGC 3 cut(s) 493, 536, 1042
Hin1II CATG 5 cut(s) 175, 188, 760, 779, 814
Hin6I GCGC 3 cut(s) 491, 534, 1040
HinP1I GCGC 3 cut(s) 491, 534, 1040
HincII GTYRAC 1 cut(s) 750
HindII GTYRAC 1 cut(s) 750
HindIII AAGCTT 2 cut(s) 141, 722
HinfI GANTC 5 cut(s) 383, 443, 464, 1100, 1240
HpaII CCGG 4 cut(s) 46, 312, 447, 650
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 3 cut(s) 9, 396, 750
Hpy188I TCNGA 6 cut(s) 157, 198, 340, 856, 916, 1265
Hpy188III TCNNGA 3 cut(s) 46, 67, 605
Hpy8I GTNNAC 3 cut(s) 9, 396, 750
Hpy99I CGWCG 1 cut(s) 737
HpyAV CCTTC 3 cut(s) 341, 1135, 1230
HpyCH4III ACNGT 5 cut(s) 246, 281, 290, 661, 995
HpyCH4IV ACGT 5 cut(s) 735, 746, 1002, 1047, 1056
HpyF10VI GCNNNNNNNGC 1 cut(s) 356
HpyF3I CTNAG 2 cut(s) 544, 915
HpySE526I ACGT 5 cut(s) 735, 746, 1002, 1047, 1056
Hsp92II CATG 5 cut(s) 175, 188, 760, 779, 814
HspAI GCGC 3 cut(s) 491, 534, 1040
Kpn2I TCCGGA 1 cut(s) 45
Ksp22I TGATCA 1 cut(s) 193
KspI CCGCGG 1 cut(s) 897
Kzo9I GATC 5 cut(s) 69, 118, 193, 601, 1265
LguI GCTCTTC 1 cut(s) 901
LmnI GCTCC 4 cut(s) 92, 857, 1183, 1224
Lsp1109I GCAGC 1 cut(s) 1198
LweI GCATC 5 cut(s) 381, 592, 920, 1054, 1075
MaeI CTAG 4 cut(s) 356, 929, 962, 1229
MaeII ACGT 5 cut(s) 735, 746, 1002, 1047, 1056
MaeIII GTNAC 8 cut(s) 187, 246, 284, 559, 970, 1003, 1057, 1127
MalI GATC 5 cut(s) 71, 120, 195, 603, 1267
MboI GATC 5 cut(s) 69, 118, 193, 601, 1265
MboII GAAGA 5 cut(s) 34, 144, 588, 918, 950
MflI RGATCY 2 cut(s) 118, 1265
MhlI GDGCHC 2 cut(s) 309, 1260
MluCI AATT 9 cut(s) 209, 258, 411, 474, 643, 667, 815, 846, 1249
MlyI GAGTC 2 cut(s) 392, 458
MmeI TCCRAC 1 cut(s) 300
Mph1103I ATGCAT 1 cut(s) 374
MroI TCCGGA 1 cut(s) 45
MroXI GAANNNNTTC 1 cut(s) 415
MseI TTAA 8 cut(s) 135, 212, 231, 432, 497, 510, 786, 1276
MslI CAYNNNNRTG 2 cut(s) 659, 834
MspA1I CMGCKG 1 cut(s) 896
MspI CCGG 4 cut(s) 46, 312, 447, 650
MspR9I CCNGG 2 cut(s) 61, 313
MvaI CCWGG 1 cut(s) 61
MvnI CGCG 5 cut(s) 493, 536, 606, 896, 987
MwoI GCNNNNNNNGC 1 cut(s) 356
NciI CCSGG 1 cut(s) 313
NdeII GATC 5 cut(s) 69, 118, 193, 601, 1265
NheI GCTAGC 1 cut(s) 355
NlaIII CATG 5 cut(s) 175, 188, 760, 779, 814
NlaIV GGNNCC 2 cut(s) 841, 1226
NmeAIII GCCGAG 1 cut(s) 1039
NmuCI GTSAC 3 cut(s) 246, 1003, 1057
NruI TCGCGA 1 cut(s) 606
NsiI ATGCAT 1 cut(s) 374
NspV TTCGAA 1 cut(s) 529
PasI CCCWGGG 1 cut(s) 60
PceI AGGCCT 1 cut(s) 330
PciSI GCTCTTC 1 cut(s) 901
PcsI WCGNNNNNNNCGW 3 cut(s) 78, 999, 1053
PdmI GAANNNNTTC 1 cut(s) 415
PfeI GAWTC 3 cut(s) 443, 1100, 1240
PflFI GACNNNGTC 1 cut(s) 954
PflMI CCANNNNNTGG 1 cut(s) 393
PkrI GCNGC 2 cut(s) 895, 1188
PleI GAGTC 2 cut(s) 391, 458
PpsI GAGTC 2 cut(s) 391, 458
Ppu21I YACGTR 1 cut(s) 1057
PpuMI RGGWCCY 1 cut(s) 109
PshAI GACNNNNGTC 1 cut(s) 101
PshBI ATTAAT 1 cut(s) 786
Psp124BI GAGCTC 1 cut(s) 309
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 2 cut(s) 841, 1226
PspPI GGNCC 3 cut(s) 109, 298, 1011
PspPPI RGGWCCY 1 cut(s) 109
PsuI RGATCY 2 cut(s) 118, 1265
PsyI GACNNNGTC 1 cut(s) 954
RruI TCGCGA 1 cut(s) 606
RsaI GTAC 3 cut(s) 271, 809, 1247
RsaNI GTAC 3 cut(s) 270, 808, 1246
RseI CAYNNNNRTG 2 cut(s) 659, 834
SacI GAGCTC 1 cut(s) 309
SacII CCGCGG 1 cut(s) 897
SapI GCTCTTC 1 cut(s) 901
SaqAI TTAA 8 cut(s) 135, 212, 231, 432, 497, 510, 786, 1276
SatI GCNGC 2 cut(s) 894, 1187
Sau3AI GATC 5 cut(s) 69, 118, 193, 601, 1265
Sau96I GGNCC 3 cut(s) 109, 298, 1011
SchI GAGTC 2 cut(s) 392, 458
ScrFI CCNGG 2 cut(s) 61, 313
SduI GDGCHC 2 cut(s) 309, 1260
SfaNI GCATC 5 cut(s) 381, 592, 920, 1054, 1075
SfcI CTRYAG 1 cut(s) 280
Sfr303I CCGCGG 1 cut(s) 897
SfuI TTCGAA 1 cut(s) 529
SgrBI CCGCGG 1 cut(s) 897
SinI GGWCC 1 cut(s) 109
SmiMI CAYNNNNRTG 2 cut(s) 659, 834
SmlI CTYRAG 2 cut(s) 251, 302
SmoI CTYRAG 2 cut(s) 251, 302
Sse9I AATT 9 cut(s) 209, 258, 411, 474, 643, 667, 815, 846, 1249
SseBI AGGCCT 1 cut(s) 330
SsiI CCGC 6 cut(s) 350, 493, 894, 896, 985, 1082
SspI AATATT 1 cut(s) 700
SspMI CTAG 4 cut(s) 356, 929, 962, 1229
SstI GAGCTC 1 cut(s) 309
StuI AGGCCT 1 cut(s) 330
StyD4I CCNGG 2 cut(s) 59, 311
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 5 cut(s) 246, 281, 290, 661, 995
TaiI ACGT 5 cut(s) 738, 749, 1005, 1050, 1059
TaqI TCGA 3 cut(s) 72, 529, 732
TaqII GACCGA 1 cut(s) 336
TasI AATT 9 cut(s) 209, 258, 411, 474, 643, 667, 815, 846, 1249
TatI WGTACW 1 cut(s) 1245
TauI GCSGC 1 cut(s) 896
TfiI GAWTC 3 cut(s) 443, 1100, 1240
Tru1I TTAA 8 cut(s) 135, 212, 231, 432, 497, 510, 786, 1276
Tru9I TTAA 8 cut(s) 135, 212, 231, 432, 497, 510, 786, 1276
TscAI CASTG 4 cut(s) 97, 710, 838, 1110
TseFI GTSAC 3 cut(s) 246, 1003, 1057
TseI GCWGC 1 cut(s) 1186
Tsp45I GTSAC 3 cut(s) 246, 1003, 1057
TspDTI ATGAA 6 cut(s) 65, 160, 173, 251, 408, 764
TspGWI ACGGA 2 cut(s) 131, 1076
TspRI CASTG 4 cut(s) 97, 710, 838, 1110
Tth111I GACNNNGTC 1 cut(s) 954
Van91I CCANNNNNTGG 1 cut(s) 393
VpaK11BI GGWCC 1 cut(s) 109
VspI ATTAAT 1 cut(s) 786
XapI RAATTY 1 cut(s) 815
XmnI GAANNNNTTC 1 cut(s) 415
XspI CTAG 4 cut(s) 356, 929, 962, 1229
Zsp2I ATGCAT 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.