pycom06g14860

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0011354
Physical Location & Seq
Reverse (-)
19484967 .. 19492471
7505 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3072 bp
ATGAGGCTTTACGTCTACGTGATGGAAGCTCAGGACTTGCCCGTGAGGGAGTCGTACGTGAAGCTCCAGGTGCGGAGGCACAAGTCCAAGACCCGGGTTTTGAGAAGTACCACCAACCCGGTTTGGAATGAGGAGTTTGTTTTCGGGGTGCATGATGTGAATGAGGAGCTTGTGGTGTCTGTGTATCACCAGGATGAGGAGCAGTCTGGCTTGTTCTATGGCGGGTCTGAGTTGGTGGGTCGGGTTTCGATTCCGGTTTCGTATGTTGCTGCTGAGAGTACTTGCACCTTGCCTCCGGCTTGGTATGCTCTTGAAAGTCCCAGGACTGGGAAGTTCATTAGCAAAGATTGTGGGAAAATTCTTCTCACCCTTTCTCTGCATGAAAAAGGCCATGATGCTTCCATTGATCACGTTCTTAGTGATCATTCAAATATAAGCCTTGAGGAACCTAATGAAGTGGACAGTCCATTTATGTCAACTCACGGTGTCTTTTCTTCCAAATCTTTATGTTCCAAGATGCCAGGTGGTATACCGTTGTTGAAGGCTATTGTGAGATGTATGGACAAGCTTATGCATAAGAACGATGAAGCCTCAAAAACAGATGATTCTTCGGAGTTGTCTACTTCAGTATCTGATTATGAAGATTGTGGGGGGGAGCATCAAAGTAGTTGTAGCTTTGAAGAGTCTCTTGAGATGATGCGATCAAGAGGAAGCGAACAAAAACTGCCGGAAAAACTTCTGGGAGGGATTCTTATTGATCAGGAATATGCTGTTTCACGGCATGATCTTAATGCATCTCTTTTTACCCCAAATTCACAGTTTTGGAGAGATCTTGCAGAACTTCAGGGGACAACAGATCTACAGGAGGGGCCTTGGATGTGGAAATCCGGAGAGATGCCATGTTTGACACGAGTTGTTTCCTACACAAGAGCTGCCTCAAAGTTAGTTAAGGCTGTTAAAGCCACAGAGGAGCAAACGTACATCACAGCAGATGGAAAGGCATTTGCTGTATTAATCAGTGTGAGTACACCTGATGTTCCATATGGAAATTCATTCAAGATTCAGTTGCTTTACAAGATAATGTCTGGACCAGAGCTACCTTCATTGGAACAATCCTCCCGTCTGGTGGTATCCTGGGACATTAACTTTCTCCAGAACACAATGATGAAAGGCATGATTGAGCGAGGAGTTCGACAGGGACTTGAGGAGAGTTTTGGTCAGTTTTCCAGCTTGCTGGCTCGGAATTTCAAAATGCTGGATTCAACAGACTTGTCTGACAAGGATCATATCCTGGCAAGTATGCAGGCAGGGCAACAATCAGATTTGAAGTTGGCAACTGAATACTTCTGGAATTTCACTCTATTTACCACTATCTTTATGGTTCTGTATGTCCTGGTTCATATTCTACTTTGTGAGCCCAGTACACCCCAGGGCTTGGAATTTATCGGACTTGATTTGCCAGACAGTTTTGGAGAGCTCATCACATGTGGAATTCTCATCCTTCAGTTGGAGCGCGTTTATAACATGGTTTTACACTTTGTACAAGCTAGGTTGCAGAAAGGAAGTGATAATGGAGTCAAAGCACAAGGTGATGGATGGGTTCTTACTGTTGCTTTGATTGAAGGGAACAGCTTAGCATCCTTGGATTCATCAGGGTTCTCTGATCCCTATGTGGTTTTCACCTGCAATGGTAAAACAAGAGCAAGCTCTGTCAAGCTTCAAACAAGTGATCCTCAATGGAACGATATACTTGAGTTTGGTGCTATGGAAGAACCGCCATCAGTTCTGGATGTTGAAGTTTTTGATTTTGATGGCCCATTTGACCGAGCTACATCACTTGGACATGCTGAGATCAATTTTCTTAAACACTCAGCTACCGAACTTGCAGATTTGTGGGTCTCCCTTGACGGAAAGCTTGCTCAGTCTTCTCAATCAAAGTTGCACGTCAGAATCTTTTTGGACAATAACAAGGGAGCTGAAACAATTAGGGATTATATGACCAAAATGGAAAAGGAAGTTGGAAAGAAGTTGAATCTGCGATCACCTCAGAGGAATTCAACATTTCAGAAATTGTTTGGGTTGCCACCAGAAGAATTTCTTATCAGTGATTTTACATGCTCCCTGAGGAGGAAAATGCCTTTACAGGGCCGACTCTTTCTATCTGCAAGGATAGTTGGGTTTTACTCGAACTTGTTTGGACACAAAACAAAATTTTTCTTTCTTTGGGAGGATATTGAAGATATCCAAGTTCTTCCCCCATCCTTGTCATCTGTGGGTAGCCCCTTGCTGGCCTTTGTTCTAAAAAAGGATCGAGGAATTGATGCAAGGCATGGTGCGAAGTGTCAAGACGATGAAGGCAGGCTGAAATTTCATTTCCAATCATTTGTATCATCTAATTCAGCCAGCAGGACAATAATGGGCTTGTGGAGAATGAGAACACTTAGTGCTGATCAGAAAACACAAATTGCTGAAGGACATTTTGATCAGAAGGACATCCCCATAATGCTTGAAGACACTGAATCCATACTCAATCTTGAGTATGAAAAGATGTCCAAAGTTTATACTGCAGAACTACCTATTAACACAAAATCTCTGATGGAAATTTTCGAGGGAGGAAAGTTGGAGCATAAAATCATGGGGAAATCATGCTGTCTTAACTATGTAACTACCGCTTGGGAACCTACAAAAAAGCCGGATGTCTTTGAACGACAACTATCTTACAGGTTCAACCGCCAGGTCTCGATCTTTGGAGGAGAGGTTACATGCAGACAAGAAAAGTCCCCCTTGGCAAACGGTGAAGGATGGATCGTGGATGAAGTTATGGCTCTGCACGGCATTCCATTTGAGGATCACTTTCGTGTTCAGCTCAGGTACCGCATCGAGAAATCTGCCATGGCTCATAATGCATGTAAATGCAATGTTCATGTCAGGGTCTTCTGGCTTAAAAGGACAAAGTTTCAGGACAGGGTCACACGCAACATCATAGGGAAATTCGCCCATGGATTCAAGGAAATATTCGAATTTGTCGAGCGGGAGATTCTCTTGGCATCGGGACAAGACATTTCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1024

Amino Acids

115.56

Weight (kDa)

5.74

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 90 3.1e-18 C2 domain
VASt PF16016 251 - 400 9.8e-33 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 534 - 635 2.4e-14 C2 domain
GRAM PF02893 687 - 788 6.3e-18 GRAM domain
VASt PF16016 853 - 995 7.2e-22 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50170
fragaria_vesca FvH4_5g06260 FvH4_5g06260 FvH4_5g06260
malus_domestica MD06G1166100.v1.1
prunus_persica Prupe.5G166000_v2.0.a1
pyrus_communis pycom06g14860
rosa_chinensis RchiOBHm_Chr7g0192601
rosa_laevigata RLG00000004348
rosa_multiflora Rmu_sc0004161.1_g000002
rosa_roxburghii Rroxscaffold_3G00262850
rosa_rugosa Rorug07G0002600 Rorug07G0002700 Rorug07G0002800
rosa_samantha Rh7AG127000 Rh7BG129600 Rh7CG131100 Rh7CG132000 Rh7DG130600
rosa_wichuraiana Rw7G010940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1521
AarI CACCTGC 1 cut(s) 1691
Acc36I ACCTGC 1 cut(s) 1691
Acc65I GGTACC 1 cut(s) 2871
AccB1I GGYRCC 1 cut(s) 2871
AccB7I CCANNNNNTGG 1 cut(s) 1435
AccBSI CCGCTC 1 cut(s) 3031
AccI GTMKAC 3 cut(s) 15, 529, 620
AccII CGCG 1 cut(s) 1515
AccIII TCCGGA 1 cut(s) 887
AciI CCGC 7 cut(s) 73, 222, 1775, 2670, 2731, 2875, 3031
AclWI GGATC 6 cut(s) 1290, 1658, 1724, 2316, 2813, 2856
AcuI CTGAAG 4 cut(s) 609, 827, 1487, 2490
AdeI CACNNNGTG 2 cut(s) 1589, 2444
AfaI GTAC 8 cut(s) 56, 109, 280, 980, 1027, 1423, 1542, 2873
AflIII ACRYGT 1 cut(s) 1484
AjiI CACGTC 1 cut(s) 1945
AjnI CCWGG 9 cut(s) 66, 189, 320, 520, 1133, 1290, 1392, 1428, 2733
AjuI GAANNNNNNNTTGG 2 cut(s) 2001, 2033
AleI CACNNNNGTG 1 cut(s) 2856
Alw21I GWGCWC 1 cut(s) 1479
Alw26I GTCTC 3 cut(s) 690, 1903, 2743
AlwI GGATC 6 cut(s) 1290, 1658, 1724, 2316, 2813, 2856
AlwNI CAGNNNCTG 1 cut(s) 632
Ama87I CYCGRG 1 cut(s) 93
Aor13HI TCCGGA 1 cut(s) 887
AoxI GGCC 5 cut(s) 388, 869, 1813, 2146, 2289
ApeKI GCWGC 2 cut(s) 269, 932
ArsI GACNNNNNNTTYG 2 cut(s) 82, 114
AseI ATTAAT 1 cut(s) 1013
Asp700I GAANNNNTTC 2 cut(s) 735, 2094
Asp718I GGTACC 1 cut(s) 2871
AspLEI GCGC 1 cut(s) 1515
AspS9I GGNCC 4 cut(s) 869, 1088, 1814, 2146
AsuC2I CCSGG 3 cut(s) 94, 95, 119
AsuHPI GGTGA 6 cut(s) 179, 358, 1601, 1672, 2034, 2807
AsuII TTCGAA 1 cut(s) 3018
AvaI CYCGRG 1 cut(s) 93
AvaII GGWCC 1 cut(s) 1088
AxyI CCTNAGG 1 cut(s) 2123
BaeI ACNNNNGTAYC 2 cut(s) 612, 645
BanI GGYRCC 1 cut(s) 2871
BanII GRGCYC 2 cut(s) 1419, 1479
BauI CACGAG 1 cut(s) 909
BbsI GAAGAC 3 cut(s) 1917, 2517, 2926
Bbv12I GWGCWC 1 cut(s) 1479
BbvI GCAGC 2 cut(s) 256, 919
BccI CCATC 9 cut(s) 16, 986, 1586, 1590, 1786, 1805, 2266, 2590, 2796
BceAI ACGGC 2 cut(s) 794, 2848
BcgI CGANNNNNNTGC 2 cut(s) 2870, 2904
BciT130I CCWGG 9 cut(s) 68, 191, 322, 522, 1135, 1292, 1394, 1430, 2735
BciVI GTATCC 1 cut(s) 1141
BclI TGATCA 5 cut(s) 406, 421, 757, 2449, 2482
BcnI CCSGG 3 cut(s) 94, 95, 119
BcoDI GTCTC 3 cut(s) 690, 1903, 2743
BfaI CTAG 1 cut(s) 1548
BfmI CTRYAG 2 cut(s) 860, 2565
BfuAI ACCTGC 1 cut(s) 1691
BfuI GTATCC 1 cut(s) 1141
BglII AGATCT 2 cut(s) 829, 856
BisI GCNGC 2 cut(s) 270, 933
BlpI GCTNAGC 1 cut(s) 1633
BlsI GCNGC 2 cut(s) 271, 934
BmcAI AGTACT 1 cut(s) 280
Bme18I GGWCC 1 cut(s) 1088
BmeT110I CYCGRG 1 cut(s) 93
BmgBI CACGTC 1 cut(s) 1945
BmgT120I GGNCC 4 cut(s) 869, 1088, 1814, 2146
BmiI GGNNCC 4 cut(s) 447, 870, 2679, 2873
BmrI ACTGGG 2 cut(s) 336, 1413
BmuI ACTGGG 2 cut(s) 336, 1413
BpiI GAAGAC 3 cut(s) 1917, 2517, 2926
BpmI CTGGAG 2 cut(s) 50, 1136
Bpu10I CCTNAGC 2 cut(s) 30, 2867
Bpu1102I GCTNAGC 1 cut(s) 1633
Bpu14I TTCGAA 1 cut(s) 3018
BpuEI CTTGAG 5 cut(s) 461, 710, 1223, 1772, 2555
BpuMI CCSGG 3 cut(s) 94, 95, 119
BsaAI YACGTR 2 cut(s) 19, 58
BsaI GGTCTC 2 cut(s) 1903, 2743
BsaWI WCCGGW 2 cut(s) 253, 887
BsaXI ACNNNNNCTCC 6 cut(s) 277, 307, 962, 992, 2742, 2772
Bse1I ACTGG 2 cut(s) 331, 1419
Bse21I CCTNAGG 1 cut(s) 2123
Bse3DI GCAATG 2 cut(s) 1693, 2923
BseAI TCCGGA 1 cut(s) 887
BseBI CCWGG 9 cut(s) 68, 191, 322, 522, 1135, 1292, 1394, 1430, 2735
BseMI GCAATG 2 cut(s) 1693, 2923
BseMII CTCAG 9 cut(s) 44, 219, 264, 1839, 1884, 1934, 2060, 2114, 2881
BseNI ACTGG 2 cut(s) 331, 1419
BseRI GAGGAG 8 cut(s) 146, 179, 212, 983, 1200, 1220, 2140, 2766
BseXI GCAGC 2 cut(s) 256, 919
BsgI GTGCAG 1 cut(s) 2813
Bsh1236I CGCG 1 cut(s) 1515
BshFI GGCC 5 cut(s) 390, 871, 1815, 2148, 2291
BshNI GGYRCC 1 cut(s) 2871
BsiHKAI GWGCWC 1 cut(s) 1479
BsiHKCI CYCGRG 1 cut(s) 93
BsiSI CCGG 7 cut(s) 94, 119, 254, 296, 728, 888, 2693
BsiWI CGTACG 1 cut(s) 54
BslFI GGGAC 6 cut(s) 303, 862, 1151, 1212, 2764, 3066
BsmAI GTCTC 3 cut(s) 690, 1903, 2743
BsmFI GGGAC 6 cut(s) 303, 862, 1151, 1212, 2764, 3066
BsmI GAATGC 1 cut(s) 2835
BsnI GGCC 5 cut(s) 390, 871, 1815, 2148, 2291
Bso31I GGTCTC 2 cut(s) 1903, 2743
BsoBI CYCGRG 1 cut(s) 93
Bsp119I TTCGAA 1 cut(s) 3018
Bsp1286I GDGCHC 2 cut(s) 1419, 1479
Bsp13I TCCGGA 1 cut(s) 887
Bsp1407I TGTACA 1 cut(s) 1540
Bsp1720I GCTNAGC 1 cut(s) 1633
Bsp19I CCATGG 2 cut(s) 2892, 2998
BspACI CCGC 7 cut(s) 73, 222, 1775, 2670, 2731, 2875, 3031
BspANI GGCC 5 cut(s) 390, 871, 1815, 2148, 2291
BspCNI CTCAG 9 cut(s) 43, 220, 265, 1840, 1883, 1933, 2059, 2115, 2880
BspEI TCCGGA 1 cut(s) 887
BspFNI CGCG 1 cut(s) 1515
BspLI GGNNCC 4 cut(s) 447, 870, 2679, 2873
BspMAI CTGCAG 1 cut(s) 2569
BspMI ACCTGC 1 cut(s) 1691
BspPI GGATC 6 cut(s) 1290, 1658, 1724, 2316, 2813, 2856
BspT104I TTCGAA 1 cut(s) 3018
BspT107I GGYRCC 1 cut(s) 2871
BspTNI GGTCTC 2 cut(s) 1903, 2743
BsrBI CCGCTC 1 cut(s) 3031
BsrDI GCAATG 2 cut(s) 1693, 2923
BsrGI TGTACA 1 cut(s) 1540
BsrI ACTGG 2 cut(s) 331, 1419
BssNAI GTATAC 1 cut(s) 530
BssSI CACGAG 1 cut(s) 909
BssT1I CCWWGG 5 cut(s) 872, 1641, 2784, 2892, 2998
Bst1107I GTATAC 1 cut(s) 530
Bst2BI CACGAG 1 cut(s) 909
Bst2UI CCWGG 9 cut(s) 68, 191, 322, 522, 1135, 1292, 1394, 1430, 2735
Bst4CI ACNGT 7 cut(s) 464, 485, 534, 819, 1466, 1609, 2795
Bst6I CTCTTC 1 cut(s) 675
BstAUI TGTACA 1 cut(s) 1540
BstBAI YACGTR 2 cut(s) 19, 58
BstBI TTCGAA 1 cut(s) 3018
BstC8I GCNNGC 8 cut(s) 1232, 1236, 1305, 1705, 1917, 2289, 2360, 2404
BstDSI CCRYGG 2 cut(s) 2892, 2998
BstFNI CGCG 1 cut(s) 1515
BstHHI GCGC 1 cut(s) 1515
BstMAI GTCTC 3 cut(s) 690, 1903, 2743
BstMWI GCNNNNNNNGC 5 cut(s) 70, 305, 959, 1309, 2903
BstNI CCWGG 9 cut(s) 68, 191, 322, 522, 1135, 1292, 1394, 1430, 2735
BstNSI RCATGY 5 cut(s) 1488, 1847, 2118, 2766, 2910
BstSFI CTRYAG 2 cut(s) 860, 2565
BstUI CGCG 1 cut(s) 1515
BstV1I GCAGC 2 cut(s) 256, 919
BstV2I GAAGAC 3 cut(s) 1917, 2517, 2926
BstX2I RGATCY 2 cut(s) 829, 856
BstXI CCANNNNNNTGG 1 cut(s) 1234
BstYI RGATCY 2 cut(s) 829, 856
BstZ17I GTATAC 1 cut(s) 530
Bsu36I CCTNAGG 1 cut(s) 2123
BsuI GTATCC 1 cut(s) 1141
BsuRI GGCC 5 cut(s) 390, 871, 1815, 2148, 2291
BtgI CCRYGG 2 cut(s) 2892, 2998
BtrI CACGTC 1 cut(s) 1945
BtsIMutI CAGTG 3 cut(s) 1024, 2110, 2514
BveI ACCTGC 1 cut(s) 1691
Cac8I GCNNGC 8 cut(s) 1232, 1236, 1305, 1705, 1917, 2289, 2360, 2404
CaiI CAGNNNCTG 1 cut(s) 632
CfoI GCGC 1 cut(s) 1515
Cfr13I GGNCC 4 cut(s) 869, 1088, 1814, 2146
Cfr9I CCCGGG 1 cut(s) 93
Csp6I GTAC 8 cut(s) 55, 108, 279, 979, 1026, 1422, 1541, 2872
CspCI CAANNNNNGTGG 2 cut(s) 331, 366
CviQI GTAC 8 cut(s) 55, 108, 279, 979, 1026, 1422, 1541, 2872
DraIII CACNNNGTG 2 cut(s) 1589, 2444
Eam1104I CTCTTC 1 cut(s) 675
EarI CTCTTC 1 cut(s) 675
Ecl136II GAGCTC 1 cut(s) 1477
Eco130I CCWWGG 5 cut(s) 872, 1641, 2784, 2892, 2998
Eco24I GRGCYC 2 cut(s) 1419, 1479
Eco31I GGTCTC 2 cut(s) 1903, 2743
Eco32I GATATC 1 cut(s) 2242
Eco47I GGWCC 1 cut(s) 1088
Eco53kI GAGCTC 1 cut(s) 1477
Eco57I CTGAAG 4 cut(s) 609, 827, 1487, 2490
Eco81I CCTNAGG 1 cut(s) 2123
Eco88I CYCGRG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 1477
EcoO109I RGGNCCY 1 cut(s) 869
EcoRI GAATTC 2 cut(s) 1491, 2053
EcoRII CCWGG 9 cut(s) 66, 189, 320, 520, 1133, 1290, 1392, 1428, 2733
EcoRV GATATC 1 cut(s) 2242
EcoT14I CCWWGG 5 cut(s) 872, 1641, 2784, 2892, 2998
EcoT22I ATGCAT 3 cut(s) 576, 796, 2908
EcoT38I GRGCYC 2 cut(s) 1419, 1479
ErhI CCWWGG 5 cut(s) 872, 1641, 2784, 2892, 2998
FalI AAGNNNNNCTT 6 cut(s) 672, 704, 2082, 2114, 2768, 2800
FaqI GGGAC 6 cut(s) 303, 862, 1151, 1212, 2764, 3066
FauI CCCGC 2 cut(s) 215, 3024
FauNDI CATATG 1 cut(s) 1042
FbaI TGATCA 5 cut(s) 406, 421, 757, 2449, 2482
FblI GTMKAC 3 cut(s) 15, 529, 620
Fnu4HI GCNGC 2 cut(s) 270, 933
FriOI GRGCYC 2 cut(s) 1419, 1479
Fsp4HI GCNGC 2 cut(s) 270, 933
FspBI CTAG 1 cut(s) 1548
GlaI GCGC 1 cut(s) 1514
GluI GCNGC 2 cut(s) 270, 933
GsuI CTGGAG 2 cut(s) 50, 1136
HaeIII GGCC 5 cut(s) 390, 871, 1815, 2148, 2291
HapII CCGG 7 cut(s) 94, 119, 254, 296, 728, 888, 2693
HhaI GCGC 1 cut(s) 1515
Hin6I GCGC 1 cut(s) 1513
HinP1I GCGC 1 cut(s) 1513
HincII GTYRAC 1 cut(s) 477
HindII GTYRAC 1 cut(s) 477
HindIII AAGCTT 3 cut(s) 566, 1715, 1913
HpaII CCGG 7 cut(s) 94, 119, 254, 296, 728, 888, 2693
HphI GGTGA 6 cut(s) 179, 358, 1601, 1672, 2034, 2807
Hpy166II GTNNAC 7 cut(s) 16, 460, 477, 530, 621, 1028, 1424
Hpy8I GTNNAC 7 cut(s) 16, 460, 477, 530, 621, 1028, 1424
HpyAV CCTTC 8 cut(s) 535, 1110, 1511, 1616, 2348, 2465, 2482, 2792
HpyCH4III ACNGT 7 cut(s) 464, 485, 534, 819, 1466, 1609, 2795
HpyCH4IV ACGT 6 cut(s) 12, 18, 57, 411, 977, 1944
HpyF10VI GCNNNNNNNGC 5 cut(s) 70, 305, 959, 1309, 2903
HpySE526I ACGT 6 cut(s) 12, 18, 57, 411, 977, 1944
HspAI GCGC 1 cut(s) 1513
Kpn2I TCCGGA 1 cut(s) 887
KpnI GGTACC 1 cut(s) 2875
Ksp22I TGATCA 5 cut(s) 406, 421, 757, 2449, 2482
LmnI GCTCC 9 cut(s) 69, 166, 199, 655, 970, 1510, 1973, 2123, 2623
Lsp1109I GCAGC 2 cut(s) 256, 919
MaeI CTAG 1 cut(s) 1548
MaeII ACGT 6 cut(s) 12, 18, 57, 411, 977, 1944
MaeIII GTNAC 3 cut(s) 2662, 2758, 2968
MbiI CCGCTC 1 cut(s) 3031
MflI RGATCY 2 cut(s) 829, 856
MhlI GDGCHC 2 cut(s) 1419, 1479
MlyI GAGTC 4 cut(s) 59, 692, 1584, 2145
MmeI TCCRAC 3 cut(s) 1488, 1999, 2601
Mph1103I ATGCAT 3 cut(s) 576, 796, 2908
MroI TCCGGA 1 cut(s) 887
MroXI GAANNNNTTC 2 cut(s) 735, 2094
MseI TTAA 9 cut(s) 789, 948, 957, 1013, 1143, 1863, 2580, 2655, 2943
MslI CAYNNNNRTG 4 cut(s) 192, 1163, 2856, 2911
MspI CCGG 7 cut(s) 94, 119, 254, 296, 728, 888, 2693
Mva1269I GAATGC 1 cut(s) 2835
MvaI CCWGG 9 cut(s) 68, 191, 322, 522, 1135, 1292, 1394, 1430, 2735
MvnI CGCG 1 cut(s) 1515
MwoI GCNNNNNNNGC 5 cut(s) 70, 305, 959, 1309, 2903
NciI CCSGG 3 cut(s) 94, 95, 119
NcoI CCATGG 2 cut(s) 2892, 2998
NdeI CATATG 1 cut(s) 1042
NlaIV GGNNCC 4 cut(s) 447, 870, 2679, 2873
NmuCI GTSAC 1 cut(s) 2968
NsiI ATGCAT 3 cut(s) 576, 796, 2908
NspI RCATGY 5 cut(s) 1488, 1847, 2118, 2766, 2910
NspV TTCGAA 1 cut(s) 3018
OliI CACNNNNGTG 1 cut(s) 2856
PaqCI CACCTGC 1 cut(s) 1691
PasI CCCWGGG 1 cut(s) 1429
PciI ACATGT 1 cut(s) 1484
PcsI WCGNNNNNNNCGW 1 cut(s) 3024
PctI GAATGC 1 cut(s) 2835
PdmI GAANNNNTTC 2 cut(s) 735, 2094
Pfl23II CGTACG 1 cut(s) 54
PflFI GACNNNGTC 1 cut(s) 2966
PflMI CCANNNNNTGG 1 cut(s) 1435
PkrI GCNGC 2 cut(s) 271, 934
PleI GAGTC 4 cut(s) 58, 691, 1583, 2145
PpsI GAGTC 4 cut(s) 58, 691, 1583, 2145
Ppu21I YACGTR 2 cut(s) 19, 58
PscI ACATGT 1 cut(s) 1484
PshBI ATTAAT 1 cut(s) 1013
PsiI TTATAA 1 cut(s) 1521
Psp124BI GAGCTC 1 cut(s) 1479
Psp6I CCWGG 9 cut(s) 66, 189, 320, 520, 1133, 1290, 1392, 1428, 2733
PspGI CCWGG 9 cut(s) 66, 189, 320, 520, 1133, 1290, 1392, 1428, 2733
PspLI CGTACG 1 cut(s) 54
PspN4I GGNNCC 4 cut(s) 447, 870, 2679, 2873
PspPI GGNCC 4 cut(s) 869, 1088, 1814, 2146
PstI CTGCAG 1 cut(s) 2569
PstNI CAGNNNCTG 1 cut(s) 632
PsuI RGATCY 2 cut(s) 829, 856
PsyI GACNNNGTC 1 cut(s) 2966
RsaI GTAC 8 cut(s) 56, 109, 280, 980, 1027, 1423, 1542, 2873
RsaNI GTAC 8 cut(s) 55, 108, 279, 979, 1026, 1422, 1541, 2872
RseI CAYNNNNRTG 4 cut(s) 192, 1163, 2856, 2911
SacI GAGCTC 1 cut(s) 1479
SaqAI TTAA 9 cut(s) 789, 948, 957, 1013, 1143, 1863, 2580, 2655, 2943
SatI GCNGC 2 cut(s) 270, 933
Sau96I GGNCC 4 cut(s) 869, 1088, 1814, 2146
ScaI AGTACT 1 cut(s) 280
SchI GAGTC 4 cut(s) 59, 692, 1584, 2145
SduI GDGCHC 2 cut(s) 1419, 1479
SfcI CTRYAG 2 cut(s) 860, 2565
SfuI TTCGAA 1 cut(s) 3018
SinI GGWCC 1 cut(s) 1088
SmaI CCCGGG 1 cut(s) 95
SmiMI CAYNNNNRTG 4 cut(s) 192, 1163, 2856, 2911
SmlI CTYRAG 5 cut(s) 440, 689, 1202, 1751, 2534
SmoI CTYRAG 5 cut(s) 440, 689, 1202, 1751, 2534
SsiI CCGC 7 cut(s) 73, 222, 1775, 2670, 2731, 2875, 3031
SspI AATATT 1 cut(s) 3015
SspMI CTAG 1 cut(s) 1548
SstI GAGCTC 1 cut(s) 1479
StyI CCWWGG 5 cut(s) 872, 1641, 2784, 2892, 2998
TaaI ACNGT 7 cut(s) 464, 485, 534, 819, 1466, 1609, 2795
TaiI ACGT 6 cut(s) 15, 21, 60, 414, 980, 1947
TaqI TCGA 1 cut(s) 248
TaqII GACCGA 1 cut(s) 1839
TatI WGTACW 4 cut(s) 278, 1025, 1421, 1540
Tru1I TTAA 9 cut(s) 789, 948, 957, 1013, 1143, 1863, 2580, 2655, 2943
Tru9I TTAA 9 cut(s) 789, 948, 957, 1013, 1143, 1863, 2580, 2655, 2943
TscAI CASTG 3 cut(s) 1024, 2110, 2521
TseFI GTSAC 1 cut(s) 2968
TseI GCWGC 2 cut(s) 269, 932
Tsp45I GTSAC 1 cut(s) 2968
TspGWI ACGGA 1 cut(s) 1923
TspMI CCCGGG 1 cut(s) 93
TspRI CASTG 3 cut(s) 1024, 2110, 2521
Tth111I GACNNNGTC 1 cut(s) 2966
Van91I CCANNNNNTGG 1 cut(s) 1435
VpaK11BI GGWCC 1 cut(s) 1088
VspI ATTAAT 1 cut(s) 1013
XceI RCATGY 5 cut(s) 1488, 1847, 2118, 2766, 2910
XcmI CCANNNNNNNNNTGG 1 cut(s) 1375
XmaI CCCGGG 1 cut(s) 93
XmiI GTMKAC 3 cut(s) 15, 529, 620
XmnI GAANNNNTTC 2 cut(s) 735, 2094
XspI CTAG 1 cut(s) 1548
ZrmI AGTACT 1 cut(s) 280
Zsp2I ATGCAT 3 cut(s) 576, 796, 2908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.