pycom06g15880

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012810
Physical Location & Seq
Forward (+)
20357993 .. 20359531
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 591 bp
ATGGGAAATTGTCAGGCAATTGATGCAGCAACACTGGTAGTACAACACCCAAGTGGGAAGGAAGAGAAGTTCTATGGGGAAGTGAGTGCAAGTGAGATCATGAAGATGAACCCTGGCCACTATGTTGCTCTTCTCATCTCCACCACCTTGTGTCCCTCCAATCCTACCACTGACACTGGTGGTGACCAGAAAGACAAGAATTCGAATCCAAACGACAACAAGAATATCGCTTCTTCGGTTCGAGTGACGCGCATAAAGCTCCTCAGGCCAACCGAATCTCTTGTTCTTGGCAAAGTTTACAGACTCATCGCCACTCAAGAAGTGATGAAGGGATTGTGGGCAAAGAAACAAGGGAAGGTGAAGAGAAATAACAATCATCAGGTGGAAGGAGAGCAGCAGCCACAAGTAGAGAGGGTGATGAGAGAGAAGCAATTTCTGACATCAGGCAGAAGATCTGAGACTTCTGAGCTGCTGGAGAAGGACATCAAGGTGAATAACAAACGTGGGAGAGGAGGATACCGACAAAGAACAGCAACGACATCAAGAACGTGGCAGCCCTCATTACACAGCATCTCAGAGGCTGCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

197

Amino Acids

21.86

Weight (kDa)

9.81

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 193 5.4e-23 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012810)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50090 AT5G50090 AT5G50090
fragaria_vesca FvH4_5g16570
malus_domestica MD06G1178700.v1.1 MD14G1185100.v1.1
prunus_persica Prupe.5G177800_v2.0.a1
pyrus_communis pycom06g15880 pycom14g15400
rosa_chinensis RchiOBHm_Chr7g0179551
rosa_multiflora Rmu_sc0005329.1_g000004
rosa_roxburghii Rroxscaffold_3G00273720
rosa_rugosa Rorug06G0425500
rosa_samantha Rh7AG025300 Rh7BG024800 Rh7CG026100 Rh7DG026100
rosa_wichuraiana Rw7G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 250
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 1 cut(s) 199
AdeI CACNNNGTG 1 cut(s) 150
AfaI GTAC 1 cut(s) 42
AjnI CCWGG 1 cut(s) 112
AleI CACNNNNGTG 1 cut(s) 51
AloI GAACNNNNNNTCC 2 cut(s) 53, 85
AluBI AGCT 2 cut(s) 259, 469
AluI AGCT 2 cut(s) 259, 469
Alw26I GTCTC 1 cut(s) 452
AlwNI CAGNNNCTG 1 cut(s) 581
AoxI GGCC 2 cut(s) 115, 266
ApeKI GCWGC 6 cut(s) 26, 394, 397, 469, 553, 581
ApoI RAATTY 1 cut(s) 199
AspLEI GCGC 1 cut(s) 252
AsuHPI GGTGA 4 cut(s) 194, 370, 427, 502
AsuII TTCGAA 1 cut(s) 203
AxyI CCTNAGG 1 cut(s) 263
BalI TGGCCA 1 cut(s) 117
BbvI GCAGC 6 cut(s) 38, 406, 409, 456, 565, 568
BciT130I CCWGG 1 cut(s) 114
BciVI GTATCC 1 cut(s) 509
BcoDI GTCTC 1 cut(s) 452
BfuI GTATCC 1 cut(s) 509
BglII AGATCT 1 cut(s) 452
BisI GCNGC 6 cut(s) 27, 395, 398, 470, 554, 582
BlsI GCNGC 6 cut(s) 28, 396, 399, 471, 555, 583
Bme1390I CCNGG 1 cut(s) 114
BmrFI CCNGG 1 cut(s) 114
BmsI GCATC 2 cut(s) 13, 579
BpmI CTGGAG 1 cut(s) 494
Bpu14I TTCGAA 1 cut(s) 203
BpuEI CTTGAG 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 112
Bse1I ACTGG 2 cut(s) 39, 181
Bse21I CCTNAGG 1 cut(s) 263
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 112
BseMII CTCAG 4 cut(s) 277, 447, 456, 588
BseNI ACTGG 2 cut(s) 39, 181
BseRI GAGGAG 2 cut(s) 251, 525
BseXI GCAGC 6 cut(s) 38, 406, 409, 456, 565, 568
Bsh1236I CGCG 1 cut(s) 250
BshFI GGCC 2 cut(s) 117, 268
BslFI GGGAC 1 cut(s) 138
BsmAI GTCTC 1 cut(s) 452
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 2 cut(s) 117, 268
Bsp119I TTCGAA 1 cut(s) 203
Bsp143I GATC 2 cut(s) 96, 452
BspANI GGCC 2 cut(s) 117, 268
BspCNI CTCAG 4 cut(s) 276, 448, 457, 587
BspFNI CGCG 1 cut(s) 250
BspHI TCATGA 1 cut(s) 99
BspQI GCTCTTC 1 cut(s) 135
BspT104I TTCGAA 1 cut(s) 203
BsrI ACTGG 2 cut(s) 39, 181
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 2 cut(s) 96, 452
Bst2UI CCWGG 1 cut(s) 114
Bst6I CTCTTC 3 cut(s) 57, 135, 356
BstAPI GCANNNNNTGC 1 cut(s) 23
BstBI TTCGAA 1 cut(s) 203
BstDEI CTNAG 4 cut(s) 263, 456, 465, 574
BstEII GGTNACC 1 cut(s) 182
BstFNI CGCG 1 cut(s) 250
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 2 cut(s) 99, 455
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 2 cut(s) 96, 452
BstMWI GCNNNNNNNGC 3 cut(s) 23, 256, 265
BstNI CCWGG 1 cut(s) 114
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 1 cut(s) 112
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 6 cut(s) 38, 406, 409, 456, 565, 568
BstX2I RGATCY 1 cut(s) 452
BstYI RGATCY 1 cut(s) 452
Bsu36I CCTNAGG 1 cut(s) 263
BsuI GTATCC 1 cut(s) 509
BsuRI GGCC 2 cut(s) 117, 268
BtgZI GCGATG 1 cut(s) 292
BtsIMutI CAGTG 3 cut(s) 32, 168, 174
CaiI CAGNNNCTG 1 cut(s) 581
CciI TCATGA 1 cut(s) 99
CfoI GCGC 1 cut(s) 252
CseI GACGC 1 cut(s) 256
Csp6I GTAC 1 cut(s) 41
CviAII CATG 1 cut(s) 100
CviJI RGCY 7 cut(s) 117, 259, 268, 400, 469, 556, 581
CviKI_1 RGCY 7 cut(s) 117, 259, 268, 400, 469, 556, 581
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 4 cut(s) 263, 456, 465, 574
DpnI GATC 2 cut(s) 98, 454
DpnII GATC 2 cut(s) 96, 452
DraIII CACNNNGTG 1 cut(s) 150
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 3 cut(s) 57, 135, 356
EarI CTCTTC 3 cut(s) 57, 135, 356
Eco81I CCTNAGG 1 cut(s) 263
Eco91I GGTNACC 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 112
FaeI CATG 1 cut(s) 103
FaiI YATR 4 cut(s) 75, 101, 123, 254
FaqI GGGAC 1 cut(s) 138
FatI CATG 1 cut(s) 99
Fnu4HI GCNGC 6 cut(s) 27, 395, 398, 470, 554, 582
Fsp4HI GCNGC 6 cut(s) 27, 395, 398, 470, 554, 582
GlaI GCGC 1 cut(s) 251
GluI GCNGC 6 cut(s) 27, 395, 398, 470, 554, 582
GsuI CTGGAG 1 cut(s) 494
HaeIII GGCC 2 cut(s) 117, 268
HgaI GACGC 1 cut(s) 256
HhaI GCGC 1 cut(s) 252
Hin1II CATG 1 cut(s) 103
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HinfI GANTC 3 cut(s) 205, 275, 303
HphI GGTGA 4 cut(s) 194, 370, 427, 502
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 4 cut(s) 438, 457, 466, 577
Hpy188III TCNNGA 3 cut(s) 100, 317, 543
Hpy8I GTNNAC 1 cut(s) 298
HpyAV CCTTC 5 cut(s) 52, 322, 349, 380, 472
HpyCH4IV ACGT 2 cut(s) 502, 548
HpyCH4V TGCA 3 cut(s) 26, 89, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 256, 265
HpyF3I CTNAG 4 cut(s) 263, 456, 465, 574
HpySE526I ACGT 2 cut(s) 502, 548
Hsp92II CATG 1 cut(s) 103
HspAI GCGC 1 cut(s) 250
Kzo9I GATC 2 cut(s) 96, 452
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 1 cut(s) 264
LpnPI CCDG 9 cut(s) 20, 99, 126, 162, 200, 250, 365, 429, 458
Lsp1109I GCAGC 6 cut(s) 38, 406, 409, 456, 565, 568
LweI GCATC 2 cut(s) 13, 579
MaeII ACGT 2 cut(s) 502, 548
MaeIII GTNAC 2 cut(s) 182, 244
MalI GATC 2 cut(s) 98, 454
MboI GATC 2 cut(s) 96, 452
MboII GAAGA 6 cut(s) 74, 115, 122, 225, 373, 462
MfeI CAATTG 1 cut(s) 18
MflI RGATCY 1 cut(s) 452
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 4 cut(s) 7, 18, 199, 431
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 297
MnlI CCTC 7 cut(s) 166, 272, 405, 503, 506, 568, 571
Mox20I TGGCCA 1 cut(s) 117
MscI TGGCCA 1 cut(s) 117
MslI CAYNNNNRTG 3 cut(s) 51, 104, 488
Msp20I TGGCCA 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 114
MunI CAATTG 1 cut(s) 18
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 3 cut(s) 23, 256, 265
NdeII GATC 2 cut(s) 96, 452
NlaIII CATG 1 cut(s) 103
NmuCI GTSAC 2 cut(s) 182, 244
NspV TTCGAA 1 cut(s) 203
OliI CACNNNNGTG 1 cut(s) 51
PagI TCATGA 1 cut(s) 99
PciSI GCTCTTC 1 cut(s) 135
PfeI GAWTC 2 cut(s) 205, 275
PkrI GCNGC 6 cut(s) 28, 396, 399, 471, 555, 583
PleI GAGTC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 112
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 581
PsuI RGATCY 1 cut(s) 452
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
RseI CAYNNNNRTG 3 cut(s) 51, 104, 488
SapI GCTCTTC 1 cut(s) 135
SatI GCNGC 6 cut(s) 27, 395, 398, 470, 554, 582
Sau3AI GATC 2 cut(s) 96, 452
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 1 cut(s) 114
SetI ASST 8 cut(s) 149, 261, 360, 384, 471, 492, 505, 551
SfaNI GCATC 2 cut(s) 13, 579
SfuI TTCGAA 1 cut(s) 203
SmiMI CAYNNNNRTG 3 cut(s) 51, 104, 488
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
Sse9I AATT 4 cut(s) 7, 18, 199, 431
StyD4I CCNGG 1 cut(s) 112
TaiI ACGT 2 cut(s) 505, 551
TaqI TCGA 2 cut(s) 203, 241
TasI AATT 4 cut(s) 7, 18, 199, 431
TatI WGTACW 1 cut(s) 40
TfiI GAWTC 2 cut(s) 205, 275
TscAI CASTG 3 cut(s) 39, 175, 181
TseFI GTSAC 2 cut(s) 182, 244
TseI GCWGC 6 cut(s) 26, 394, 397, 469, 553, 581
Tsp45I GTSAC 2 cut(s) 182, 244
TspDTI ATGAA 3 cut(s) 116, 122, 341
TspRI CASTG 3 cut(s) 39, 175, 181
XapI RAATTY 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.