pycom06g18010

glycerophosphoryl diester phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0007756
Physical Location & Seq
Reverse (-)
21901946 .. 21906025
4080 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1452 bp
ATGAACATCAATAATATTTATCTCACTACTAAAAACTTGTCAAAACTCATTATACATCCACCCATTACTAATTTCCGTCAAAAGCAACCGATATCGATCCATAAATTTGCAACTTGGTTCACAGCTAGATCCACGGCGCCAAATTCTCTGGTCCCTCCTTGCTATAAAACTCTCGGTCCCACAGGATTTCACCACCTTAGGAATAAAAGGCTCTGCAGCTTAAGAAACACTGTAGGAACCAGGCAGAGAGAGAGAAGCTCTGGGAAAACGCCATGGCTTCCTCACACTTTGACAGGCTTTGCACCTCTCGTGTTCCTACTGCTCCTTGTTGGGTGTTCCGGAAGGCCAATCTGTCCGCTTCCTAGCAAAACAGCCGATGCCACCAATAGACAGCCTCTACAAACTTCTCGTCCATACAACATTGCTCATCGAGGTTCAAGTGGAGAGATTCCTGAAGAAACTGCTGCTGCATACATGAGAGCTATTGAAGAGGGTGCAGACTTCATTGAAACGGATATCTTATCTTCTAAAGACGGTGTTCTTATATGCTTTCATGATGTGACCCTTGATGATACAACTGATGTTGGCGAACACAAGGAATTTGCAGATCGTAAAAGAACCTATGAAGTTCAAGGGATCAACATTACCGGTTTTTTTACTGTTGATTTTACACTAAAAGAACTGAAGTCATTACGAGTGAAGCAGAGATACCCATTTCGGGATCGACAATATAATGGAAAATATCCGATTATTACTTTTGAAGAGTACATTTCAATCGCACTGGACGCACCCAGAGTTGTTGGAATATATCCGGAGATTAAAAATCCAGTATTGATCAACCAACATGTGAAGTGGGCAGATGGTAAGAGGTTTGAGGACAAATTTGTGGAGACACTTAAGAAGTACGGATACAAGGGTTCATACCTATCAAAAGATTGGTTGAAACAACCTGCATTTATCCAGTCATTTGCTCCAACTTCACTTGTATACATATCAAATCTGACAGACTTGCCCAAGATCTTGTTAATTGCCGAAGTTACTCTTCCAACGCAAGACACTAATCAGTCCTATTGGGAAATTACTTCGGATCCTTACCTTGACTACATTAAAGATTATGTGGTGGGCATTGGACCGTGGAAAGATACTATAGTTCCTGTAGTAAACAATTATTTGCAAACACCTACCGATTTGGTCTCCAGAGCCCATGCACATGACCTACAGGTGCACCCCTACACGTACAGAAACGAGAACTCATTCCTGCACTTGAACTTTCATCAAGATCGATACGCAGAGTACAATTACTGGATTAACGAGATAGGTGTCGATGGACTTTTTACAGACTTCACAGGAAGCCTACATAACTTTCAAGAGTGGACCTCCTTCAATGACAAGAATGCATCAAGTTTATTGCACAAAATAGCATCAATGGTCTCTTCCTACAAGAAGGCGTGA

Protein Analysis

484

Amino Acids

55.33

Weight (kDa)

8.78

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDPD PF03009 143 - 450 2.1e-53 Glycerophosphoryl diester phosphodiesterase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 958
AccB1I GGYRCC 1 cut(s) 136
AccI GTMKAC 1 cut(s) 987
AccIII TCCGGA 2 cut(s) 338, 811
AciI CCGC 1 cut(s) 356
AclWI GGATC 6 cut(s) 91, 123, 644, 729, 1082, 1095
AcsI RAATTY 4 cut(s) 104, 142, 599, 881
AcuI CTGAAG 2 cut(s) 474, 704
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 4 cut(s) 767, 905, 1238, 1295
AfiI CCNNNNNNNGG 1 cut(s) 718
AflII CTTAAG 2 cut(s) 220, 896
AflIII ACRYGT 2 cut(s) 844, 1233
AgeI ACCGGT 1 cut(s) 647
AjnI CCWGG 1 cut(s) 239
AluBI AGCT 4 cut(s) 125, 219, 258, 482
AluI AGCT 4 cut(s) 125, 219, 258, 482
Alw21I GWGCWC 1 cut(s) 1227
Alw26I GTCTC 3 cut(s) 884, 1198, 1435
Alw44I GTGCAC 1 cut(s) 1223
AlwI GGATC 6 cut(s) 91, 123, 644, 729, 1082, 1095
Aor13HI TCCGGA 2 cut(s) 338, 811
AoxI GGCC 1 cut(s) 344
ApaLI GTGCAC 1 cut(s) 1223
ApeKI GCWGC 3 cut(s) 216, 464, 467
ApoI RAATTY 4 cut(s) 104, 142, 599, 881
ArsI GACNNNNNNTTYG 2 cut(s) 394, 426
AsiGI ACCGGT 1 cut(s) 647
Asp700I GAANNNNTTC 1 cut(s) 1253
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 4 cut(s) 151, 176, 1130, 1374
AsuHPI GGTGA 1 cut(s) 182
AvaII GGWCC 4 cut(s) 151, 176, 1130, 1374
AxyI CCTNAGG 1 cut(s) 197
BaeGI GKGCMC 1 cut(s) 1227
BaeI ACNNNNGTAYC 4 cut(s) 901, 934, 1134, 1167
BamHI GGATCC 1 cut(s) 1087
BanI GGYRCC 1 cut(s) 136
BanII GRGCYC 1 cut(s) 1204
BarI GAAGNNNNNNTAC 4 cut(s) 692, 724, 893, 925
BauI CACGAG 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 1227
BbvI GCAGC 3 cut(s) 228, 451, 454
BccI CCATC 2 cut(s) 854, 1319
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 1 cut(s) 241
BciVI GTATCC 1 cut(s) 902
BclI TGATCA 1 cut(s) 834
BcoDI GTCTC 3 cut(s) 884, 1198, 1435
BfaI CTAG 2 cut(s) 126, 363
BfmI CTRYAG 5 cut(s) 214, 231, 1146, 1155, 1217
BfoI RGCGCY 1 cut(s) 140
BfrI CTTAAG 2 cut(s) 220, 896
BfuAI ACCTGC 1 cut(s) 958
BfuI GTATCC 1 cut(s) 902
BglII AGATCT 1 cut(s) 1017
BisI GCNGC 3 cut(s) 217, 465, 468
BlsI GCNGC 3 cut(s) 218, 466, 469
Bme1390I CCNGG 1 cut(s) 241
Bme18I GGWCC 4 cut(s) 151, 176, 1130, 1374
BmgT120I GGNCC 4 cut(s) 151, 176, 1130, 1374
BmiI GGNNCC 5 cut(s) 138, 153, 178, 238, 1089
BmrFI CCNGG 1 cut(s) 241
BmsI GCATC 3 cut(s) 367, 1406, 1430
BplI GAGNNNNNCTC 4 cut(s) 242, 274, 1361, 1393
BpmI CTGGAG 1 cut(s) 1180
Bsa29I ATCGAT 2 cut(s) 95, 1282
BsaAI YACGTR 1 cut(s) 1236
BsaBI GATNNNNATC 1 cut(s) 95
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 2 cut(s) 1198, 1435
BsaJI CCNNGG 3 cut(s) 132, 272, 1133
BsaWI WCCGGW 3 cut(s) 338, 647, 811
Bsc4I CCNNNNNNNGG 1 cut(s) 718
Bse118I RCCGGY 1 cut(s) 647
Bse1I ACTGG 4 cut(s) 786, 827, 961, 1307
Bse21I CCTNAGG 1 cut(s) 197
Bse3DI GCAATG 1 cut(s) 420
Bse8I GATNNNNATC 1 cut(s) 95
BseAI TCCGGA 2 cut(s) 338, 811
BseBI CCWGG 1 cut(s) 241
BseCI ATCGAT 2 cut(s) 95, 1282
BseDI CCNNGG 3 cut(s) 132, 272, 1133
BseGI GGATG 1 cut(s) 55
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 718
BseMI GCAATG 1 cut(s) 420
BseNI ACTGG 4 cut(s) 786, 827, 961, 1307
BseSI GKGCMC 1 cut(s) 1227
BseXI GCAGC 3 cut(s) 228, 451, 454
BsgI GTGCAG 2 cut(s) 516, 1244
BshFI GGCC 1 cut(s) 346
BshNI GGYRCC 1 cut(s) 136
BshTI ACCGGT 1 cut(s) 647
BshVI ATCGAT 2 cut(s) 95, 1282
BsiHKAI GWGCWC 1 cut(s) 1227
BsiSI CCGG 3 cut(s) 339, 648, 812
BslFI GGGAC 2 cut(s) 137, 162
BslI CCNNNNNNNGG 1 cut(s) 718
BsmAI GTCTC 3 cut(s) 884, 1198, 1435
BsmFI GGGAC 2 cut(s) 137, 162
BsmI GAATGC 1 cut(s) 1399
BsnI GGCC 1 cut(s) 346
Bso31I GGTCTC 2 cut(s) 1198, 1435
Bsp1286I GDGCHC 2 cut(s) 1204, 1227
Bsp13I TCCGGA 2 cut(s) 338, 811
Bsp143I GATC 9 cut(s) 96, 128, 607, 636, 721, 834, 1017, 1087, 1279
Bsp19I CCATGG 1 cut(s) 272
BspACI CCGC 1 cut(s) 356
BspANI GGCC 1 cut(s) 346
BspDI ATCGAT 2 cut(s) 95, 1282
BspEI TCCGGA 2 cut(s) 338, 811
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 5 cut(s) 138, 153, 178, 238, 1089
BspMAI CTGCAG 1 cut(s) 218
BspMI ACCTGC 1 cut(s) 958
BspPI GGATC 6 cut(s) 91, 123, 644, 729, 1082, 1095
BspT107I GGYRCC 1 cut(s) 136
BspTI CTTAAG 2 cut(s) 220, 896
BspTNI GGTCTC 2 cut(s) 1198, 1435
BsrDI GCAATG 1 cut(s) 420
BsrFI RCCGGY 1 cut(s) 647
BsrI ACTGG 4 cut(s) 786, 827, 961, 1307
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 3 cut(s) 132, 272, 1133
BssMI GATC 9 cut(s) 96, 128, 607, 636, 721, 834, 1017, 1087, 1279
BssNAI GTATAC 1 cut(s) 988
BssNI GRCGYC 1 cut(s) 137
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 272
Bst1107I GTATAC 1 cut(s) 988
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 4 cut(s) 232, 536, 661, 1134
Bst6I CTCTTC 4 cut(s) 483, 756, 1047, 1438
BstACI GRCGYC 1 cut(s) 137
BstAFI CTTAAG 2 cut(s) 220, 896
BstBAI YACGTR 1 cut(s) 1236
BstDEI CTNAG 1 cut(s) 197
BstDSI CCRYGG 3 cut(s) 132, 272, 1133
BstF5I GGATG 1 cut(s) 55
BstH2I RGCGCY 1 cut(s) 140
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 9 cut(s) 99, 131, 610, 639, 724, 837, 1020, 1090, 1282
BstMAI GTCTC 3 cut(s) 884, 1198, 1435
BstMBI GATC 9 cut(s) 96, 128, 607, 636, 721, 834, 1017, 1087, 1279
BstMWI GCNNNNNNNGC 1 cut(s) 785
BstNI CCWGG 1 cut(s) 241
BstNSI RCATGY 1 cut(s) 848
BstSCI CCNGG 1 cut(s) 239
BstSFI CTRYAG 5 cut(s) 214, 231, 1146, 1155, 1217
BstSLI GKGCMC 1 cut(s) 1227
BstV1I GCAGC 3 cut(s) 228, 451, 454
BstX2I RGATCY 3 cut(s) 128, 1017, 1087
BstYI RGATCY 3 cut(s) 128, 1017, 1087
BstZ17I GTATAC 1 cut(s) 988
Bsu15I ATCGAT 2 cut(s) 95, 1282
Bsu36I CCTNAGG 1 cut(s) 197
BsuI GTATCC 1 cut(s) 902
BsuRI GGCC 1 cut(s) 346
BsuTUI ATCGAT 2 cut(s) 95, 1282
BtgI CCRYGG 3 cut(s) 132, 272, 1133
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 2 cut(s) 228, 779
BveI ACCTGC 1 cut(s) 958
CciI TCATGA 1 cut(s) 553
CfoI GCGC 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 647
Cfr13I GGNCC 4 cut(s) 151, 176, 1130, 1374
ClaI ATCGAT 2 cut(s) 95, 1282
CseI GACGC 1 cut(s) 794
Csp6I GTAC 4 cut(s) 766, 904, 1237, 1294
CspAI ACCGGT 1 cut(s) 647
CviAII CATG 6 cut(s) 273, 475, 554, 845, 1205, 1211
CviQI GTAC 4 cut(s) 766, 904, 1237, 1294
DdeI CTNAG 1 cut(s) 197
DinI GGCGCC 1 cut(s) 138
DpnI GATC 9 cut(s) 98, 130, 609, 638, 723, 836, 1019, 1089, 1281
DpnII GATC 9 cut(s) 96, 128, 607, 636, 721, 834, 1017, 1087, 1279
Eam1104I CTCTTC 4 cut(s) 483, 756, 1047, 1438
EarI CTCTTC 4 cut(s) 483, 756, 1047, 1438
Eco130I CCWWGG 1 cut(s) 272
Eco24I GRGCYC 1 cut(s) 1204
Eco31I GGTCTC 2 cut(s) 1198, 1435
Eco32I GATATC 2 cut(s) 93, 517
Eco47I GGWCC 4 cut(s) 151, 176, 1130, 1374
Eco57I CTGAAG 2 cut(s) 474, 704
Eco81I CCTNAGG 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 239
EcoRV GATATC 2 cut(s) 93, 517
EcoT14I CCWWGG 1 cut(s) 272
EcoT22I ATGCAT 1 cut(s) 1399
EcoT38I GRGCYC 1 cut(s) 1204
EgeI GGCGCC 1 cut(s) 138
EheI GGCGCC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 272
FaeI CATG 6 cut(s) 276, 478, 557, 848, 1208, 1214
FalI AAGNNNNNCTT 2 cut(s) 1026, 1058
FaqI GGGAC 2 cut(s) 137, 162
FatI CATG 6 cut(s) 272, 474, 553, 844, 1204, 1210
FbaI TGATCA 1 cut(s) 834
FblI GTMKAC 1 cut(s) 987
Fnu4HI GCNGC 3 cut(s) 217, 465, 468
FokI GGATG 1 cut(s) 42
FriOI GRGCYC 1 cut(s) 1204
Fsp4HI GCNGC 3 cut(s) 217, 465, 468
FspBI CTAG 2 cut(s) 126, 363
GlaI GCGC 1 cut(s) 138
GluI GCNGC 3 cut(s) 217, 465, 468
GsuI CTGGAG 1 cut(s) 1180
HaeII RGCGCY 1 cut(s) 140
HaeIII GGCC 1 cut(s) 346
HapII CCGG 3 cut(s) 339, 648, 812
HgaI GACGC 1 cut(s) 794
HhaI GCGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 6 cut(s) 276, 478, 557, 848, 1208, 1214
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HinfI GANTC 1 cut(s) 448
HpaII CCGG 3 cut(s) 339, 648, 812
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 5 cut(s) 120, 988, 1162, 1225, 1374
Hpy188I TCNGA 3 cut(s) 747, 1002, 1087
Hpy188III TCNNGA 8 cut(s) 339, 452, 554, 719, 812, 1197, 1277, 1367
Hpy8I GTNNAC 5 cut(s) 120, 988, 1162, 1225, 1374
HpyAV CCTTC 3 cut(s) 336, 1390, 1438
HpyCH4III ACNGT 4 cut(s) 232, 536, 661, 1134
HpyCH4IV ACGT 1 cut(s) 1235
HpyF10VI GCNNNNNNNGC 1 cut(s) 785
HpyF3I CTNAG 1 cut(s) 197
HpySE526I ACGT 1 cut(s) 1235
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 6 cut(s) 276, 478, 557, 848, 1208, 1214
HspAI GCGC 1 cut(s) 137
KasI GGCGCC 1 cut(s) 136
Kpn2I TCCGGA 2 cut(s) 338, 811
Ksp22I TGATCA 1 cut(s) 834
Kzo9I GATC 9 cut(s) 96, 128, 607, 636, 721, 834, 1017, 1087, 1279
LmnI GCTCC 2 cut(s) 327, 976
Lsp1109I GCAGC 3 cut(s) 228, 451, 454
LweI GCATC 3 cut(s) 367, 1406, 1430
MaeI CTAG 2 cut(s) 126, 363
MaeII ACGT 1 cut(s) 1235
MaeIII GTNAC 2 cut(s) 559, 1036
MalI GATC 9 cut(s) 98, 130, 609, 638, 723, 836, 1019, 1089, 1281
MboI GATC 9 cut(s) 96, 128, 607, 636, 721, 834, 1017, 1087, 1279
MboII GAAGA 6 cut(s) 467, 500, 516, 773, 1034, 1425
MflI RGATCY 3 cut(s) 128, 1017, 1087
MhlI GDGCHC 2 cut(s) 1204, 1227
MluCI AATT 9 cut(s) 70, 104, 142, 599, 881, 1026, 1077, 1165, 1297
Mly113I GGCGCC 1 cut(s) 137
MmeI TCCRAC 3 cut(s) 781, 998, 1070
MnlI CCTC 9 cut(s) 165, 291, 315, 405, 425, 484, 861, 868, 1387
Mph1103I ATGCAT 1 cut(s) 1399
MroI TCCGGA 2 cut(s) 338, 811
MroXI GAANNNNTTC 1 cut(s) 1253
MseI TTAA 6 cut(s) 221, 819, 897, 1025, 1107, 1308
MslI CAYNNNNRTG 1 cut(s) 1209
MspCI CTTAAG 2 cut(s) 220, 896
MspI CCGG 3 cut(s) 339, 648, 812
MspR9I CCNGG 1 cut(s) 241
Mva1269I GAATGC 1 cut(s) 1399
MvaI CCWGG 1 cut(s) 241
MwoI GCNNNNNNNGC 1 cut(s) 785
NarI GGCGCC 1 cut(s) 137
NcoI CCATGG 1 cut(s) 272
NdeII GATC 9 cut(s) 96, 128, 607, 636, 721, 834, 1017, 1087, 1279
NlaIII CATG 6 cut(s) 276, 478, 557, 848, 1208, 1214
NlaIV GGNNCC 5 cut(s) 138, 153, 178, 238, 1089
NmuCI GTSAC 1 cut(s) 559
NsiI ATGCAT 1 cut(s) 1399
NspI RCATGY 1 cut(s) 848
PagI TCATGA 1 cut(s) 553
PciI ACATGT 1 cut(s) 844
PctI GAATGC 1 cut(s) 1399
PdmI GAANNNNTTC 1 cut(s) 1253
PfeI GAWTC 1 cut(s) 448
PinAI ACCGGT 1 cut(s) 647
PkrI GCNGC 3 cut(s) 218, 466, 469
PluTI GGCGCC 1 cut(s) 140
Ppu21I YACGTR 1 cut(s) 1236
PscI ACATGT 1 cut(s) 844
Psp6I CCWGG 1 cut(s) 239
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 5 cut(s) 138, 153, 178, 238, 1089
PspPI GGNCC 4 cut(s) 151, 176, 1130, 1374
PstI CTGCAG 1 cut(s) 218
PsuI RGATCY 3 cut(s) 128, 1017, 1087
RsaI GTAC 4 cut(s) 767, 905, 1238, 1295
RsaNI GTAC 4 cut(s) 766, 904, 1237, 1294
RseI CAYNNNNRTG 1 cut(s) 1209
SaqAI TTAA 6 cut(s) 221, 819, 897, 1025, 1107, 1308
SatI GCNGC 3 cut(s) 217, 465, 468
Sau3AI GATC 9 cut(s) 96, 128, 607, 636, 721, 834, 1017, 1087, 1279
Sau96I GGNCC 4 cut(s) 151, 176, 1130, 1374
ScrFI CCNGG 1 cut(s) 241
SduI GDGCHC 2 cut(s) 1204, 1227
SfaNI GCATC 3 cut(s) 367, 1406, 1430
SfcI CTRYAG 5 cut(s) 214, 231, 1146, 1155, 1217
SfoI GGCGCC 1 cut(s) 138
SinI GGWCC 4 cut(s) 151, 176, 1130, 1374
SmiMI CAYNNNNRTG 1 cut(s) 1209
SmlI CTYRAG 2 cut(s) 220, 896
SmoI CTYRAG 2 cut(s) 220, 896
Sse9I AATT 9 cut(s) 70, 104, 142, 599, 881, 1026, 1077, 1165, 1297
SsiI CCGC 1 cut(s) 356
SspDI GGCGCC 1 cut(s) 136
SspI AATATT 1 cut(s) 16
SspMI CTAG 2 cut(s) 126, 363
StyD4I CCNGG 1 cut(s) 239
StyI CCWWGG 1 cut(s) 272
TaaI ACNGT 4 cut(s) 232, 536, 661, 1134
TaiI ACGT 1 cut(s) 1238
TaqI TCGA 5 cut(s) 95, 430, 724, 1282, 1323
TaqII GACCGA 1 cut(s) 164
TasI AATT 9 cut(s) 70, 104, 142, 599, 881, 1026, 1077, 1165, 1297
TatI WGTACW 2 cut(s) 765, 1293
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 6 cut(s) 221, 819, 897, 1025, 1107, 1308
Tru9I TTAA 6 cut(s) 221, 819, 897, 1025, 1107, 1308
TscAI CASTG 2 cut(s) 235, 786
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 3 cut(s) 216, 464, 467
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 6 cut(s) 17, 493, 542, 639, 909, 1262
TspGWI ACGGA 3 cut(s) 65, 527, 921
TspRI CASTG 2 cut(s) 235, 786
Vha464I CTTAAG 2 cut(s) 220, 896
VneI GTGCAC 1 cut(s) 1223
VpaK11BI GGWCC 4 cut(s) 151, 176, 1130, 1374
XapI RAATTY 4 cut(s) 104, 142, 599, 881
XceI RCATGY 1 cut(s) 848
XmiI GTMKAC 1 cut(s) 987
XmnI GAANNNNTTC 1 cut(s) 1253
XspI CTAG 2 cut(s) 126, 363
Zsp2I ATGCAT 1 cut(s) 1399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.