pycom06g20450

Protein of unknown function (DUF3007)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0007172
Physical Location & Seq
Forward (+)
23475060 .. 23477226
2167 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 555 bp
ATGCAATTGAAGGTCAGAGCTTTTCATGGTTTAGTGAGAGGAAAAGTCATGATTCACAGCTTATATAGAAATGAAACATCCCTTTTATGTGGAGAAGGATCCAATAGCATCAACCACATAATTCCAAAAAAGGCGATCTTAACCATTCCCAGATGTAGCAGTTCCTCGGACTCTAATGCAAGCGAAGATGACTCCTCTGATCAGGCCAAGAAGAAACCATTTGGATACTCAAGAAAGGATGTTTTATTGATTGGAGTAGGAGTAACTGCTATCGGGATCGGCTTGAAAAGCGGATTAGAGTTTGCTGGAGTTGATCCTTTACAAGCAGGAAACGTTGTTCAGCTGGTGCTGGTGTTGGGCCTGACTATTGGATGGATTTCCACTTATATTTTTCGTGTTTCAAATAAGGAAATGACGTACGCACAACAAATACGCGATTATGAAAGCAAAGTCATGGAGAAAAGGTTAGAAGGCCTAACAGAAGCAGAGTTAGAAGCATTGCTTGAACAAGTTGAGGAAGAGAAGAGGCGCCTCGGCGAGCAGCAGGTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3007

Protein Analysis

185

Amino Acids

20.35

Weight (kDa)

7.75

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3007 PF11460 77 - 172 9.5e-34 Protein of unknown function (DUF3007)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 535
AccB1I GGYRCC 1 cut(s) 528
AccII CGCG 1 cut(s) 435
AciI CCGC 1 cut(s) 291
AclI AACGTT 1 cut(s) 333
AclWI GGATC 4 cut(s) 93, 106, 284, 308
AcyI GRCGYC 1 cut(s) 529
AfaI GTAC 1 cut(s) 419
AgsI TTSAA 4 cut(s) 10, 286, 402, 506
AloI GAACNNNNNNTCC 2 cut(s) 321, 353
AluBI AGCT 3 cut(s) 20, 60, 343
AluI AGCT 3 cut(s) 20, 60, 343
AlwI GGATC 4 cut(s) 93, 106, 284, 308
AoxI GGCC 3 cut(s) 204, 358, 472
ApeKI GCWGC 1 cut(s) 541
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 1 cut(s) 358
BamHI GGATCC 1 cut(s) 98
BanI GGYRCC 1 cut(s) 528
BccI CCATC 1 cut(s) 366
BciVI GTATCC 1 cut(s) 218
BclI TGATCA 1 cut(s) 199
BfoI RGCGCY 1 cut(s) 532
BfuAI ACCTGC 1 cut(s) 535
BfuI GTATCC 1 cut(s) 218
BisI GCNGC 1 cut(s) 542
BlsI GCNGC 1 cut(s) 543
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 2 cut(s) 100, 530
BmsI GCATC 1 cut(s) 117
BpmI CTGGAG 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 214
BsaHI GRCGYC 1 cut(s) 529
BsaJI CCNNGG 2 cut(s) 165, 532
BsaXI ACNNNNNCTCC 4 cut(s) 246, 276, 449, 479
Bse3DI GCAATG 1 cut(s) 497
BseDI CCNNGG 2 cut(s) 165, 532
BseGI GGATG 3 cut(s) 77, 244, 377
BseMI GCAATG 1 cut(s) 497
BseRI GAGGAG 1 cut(s) 184
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 3 cut(s) 206, 360, 474
BshNI GGYRCC 1 cut(s) 528
BsiWI CGTACG 1 cut(s) 417
BsnI GGCC 3 cut(s) 206, 360, 474
Bsp143I GATC 5 cut(s) 98, 135, 199, 276, 313
BspACI CCGC 1 cut(s) 291
BspANI GGCC 3 cut(s) 206, 360, 474
BspFNI CGCG 1 cut(s) 435
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 2 cut(s) 100, 530
BspMI ACCTGC 1 cut(s) 535
BspPI GGATC 4 cut(s) 93, 106, 284, 308
BspT107I GGYRCC 1 cut(s) 528
BsrDI GCAATG 1 cut(s) 497
BssECI CCNNGG 2 cut(s) 165, 532
BssMI GATC 5 cut(s) 98, 135, 199, 276, 313
BssNI GRCGYC 1 cut(s) 529
Bst6I CTCTTC 2 cut(s) 513, 518
BstACI GRCGYC 1 cut(s) 529
BstC8I GCNNGC 2 cut(s) 181, 539
BstF5I GGATG 3 cut(s) 77, 244, 377
BstFNI CGCG 1 cut(s) 435
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 5 cut(s) 101, 138, 202, 279, 316
BstMBI GATC 5 cut(s) 98, 135, 199, 276, 313
BstMWI GCNNNNNNNGC 1 cut(s) 288
BstUI CGCG 1 cut(s) 435
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
BsuI GTATCC 1 cut(s) 218
BsuRI GGCC 3 cut(s) 206, 360, 474
BtsCI GGATG 3 cut(s) 77, 244, 377
BveI ACCTGC 1 cut(s) 535
Cac8I GCNNGC 2 cut(s) 181, 539
CciI TCATGA 1 cut(s) 48
CfoI GCGC 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 1 cut(s) 418
CviAII CATG 3 cut(s) 26, 49, 454
CviJI RGCY 7 cut(s) 20, 60, 206, 282, 343, 360, 474
CviKI_1 RGCY 7 cut(s) 20, 60, 206, 282, 343, 360, 474
CviQI GTAC 1 cut(s) 418
DinI GGCGCC 1 cut(s) 530
DpnI GATC 5 cut(s) 100, 137, 201, 278, 315
DpnII GATC 5 cut(s) 98, 135, 199, 276, 313
Eam1104I CTCTTC 2 cut(s) 513, 518
EarI CTCTTC 2 cut(s) 513, 518
Eco147I AGGCCT 1 cut(s) 474
EgeI GGCGCC 1 cut(s) 530
EheI GGCGCC 1 cut(s) 530
FaeI CATG 3 cut(s) 29, 52, 457
FaiI YATR 9 cut(s) 27, 50, 64, 66, 88, 119, 387, 441, 455
FalI AAGNNNNNCTT 4 cut(s) 122, 154, 486, 518
FatI CATG 3 cut(s) 25, 48, 453
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 1 cut(s) 542
FokI GGATG 3 cut(s) 64, 251, 384
Fsp4HI GCNGC 1 cut(s) 542
GlaI GCGC 1 cut(s) 530
GluI GCNGC 1 cut(s) 542
GsuI CTGGAG 1 cut(s) 327
HaeII RGCGCY 1 cut(s) 532
HaeIII GGCC 3 cut(s) 206, 360, 474
HhaI GCGC 1 cut(s) 531
Hin1I GRCGYC 1 cut(s) 529
Hin1II CATG 3 cut(s) 29, 52, 457
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HinfI GANTC 3 cut(s) 52, 170, 191
Hpy188I TCNGA 3 cut(s) 17, 169, 199
Hpy188III TCNNGA 3 cut(s) 49, 231, 274
HpyAV CCTTC 3 cut(s) 4, 89, 464
HpyCH4IV ACGT 2 cut(s) 333, 416
HpyCH4V TGCA 2 cut(s) 4, 179
HpyF10VI GCNNNNNNNGC 1 cut(s) 288
HpySE526I ACGT 2 cut(s) 333, 416
Hsp92I GRCGYC 1 cut(s) 529
Hsp92II CATG 3 cut(s) 29, 52, 457
HspAI GCGC 1 cut(s) 529
KasI GGCGCC 1 cut(s) 528
Ksp22I TGATCA 1 cut(s) 199
Kzo9I GATC 5 cut(s) 98, 135, 199, 276, 313
LpnPI CCDG 8 cut(s) 163, 188, 291, 312, 329, 335, 374, 530
LweI GCATC 1 cut(s) 117
MaeII ACGT 2 cut(s) 333, 416
MaeIII GTNAC 1 cut(s) 262
MalI GATC 5 cut(s) 100, 137, 201, 278, 315
MboI GATC 5 cut(s) 98, 135, 199, 276, 313
MboII GAAGA 4 cut(s) 197, 223, 530, 535
MfeI CAATTG 1 cut(s) 5
MflI RGATCY 1 cut(s) 98
MluCI AATT 2 cut(s) 5, 120
Mly113I GGCGCC 1 cut(s) 529
MlyI GAGTC 2 cut(s) 164, 185
MnlI CCTC 6 cut(s) 32, 175, 205, 508, 519, 542
MseI TTAA 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 343
MunI CAATTG 1 cut(s) 5
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 1 cut(s) 288
NarI GGCGCC 1 cut(s) 529
NdeII GATC 5 cut(s) 98, 135, 199, 276, 313
NlaIII CATG 3 cut(s) 29, 52, 457
NlaIV GGNNCC 2 cut(s) 100, 530
NmeAIII GCCGAG 1 cut(s) 513
PagI TCATGA 1 cut(s) 48
PceI AGGCCT 1 cut(s) 474
PfeI GAWTC 1 cut(s) 52
Pfl23II CGTACG 1 cut(s) 417
PkrI GCNGC 1 cut(s) 543
PleI GAGTC 2 cut(s) 164, 185
PluTI GGCGCC 1 cut(s) 532
PpsI GAGTC 2 cut(s) 164, 185
Psp1406I AACGTT 1 cut(s) 333
PspLI CGTACG 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 100, 530
PspPI GGNCC 1 cut(s) 358
PsuI RGATCY 1 cut(s) 98
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
SaqAI TTAA 1 cut(s) 140
SatI GCNGC 1 cut(s) 542
Sau3AI GATC 5 cut(s) 98, 135, 199, 276, 313
Sau96I GGNCC 1 cut(s) 358
SchI GAGTC 2 cut(s) 164, 185
SetI ASST 8 cut(s) 15, 22, 62, 336, 345, 419, 467, 549
SfaNI GCATC 1 cut(s) 117
SfoI GGCGCC 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 229
SmoI CTYRAG 1 cut(s) 229
Sse9I AATT 2 cut(s) 5, 120
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 1 cut(s) 291
SspDI GGCGCC 1 cut(s) 528
StuI AGGCCT 1 cut(s) 474
TaiI ACGT 2 cut(s) 336, 419
TasI AATT 2 cut(s) 5, 120
TfiI GAWTC 1 cut(s) 52
Tru1I TTAA 1 cut(s) 140
Tru9I TTAA 1 cut(s) 140
TseI GCWGC 1 cut(s) 541
TspDTI ATGAA 3 cut(s) 14, 87, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.