pycom06g20460

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001818
Physical Location & Seq
Forward (+)
23479564 .. 23484406
4843 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3201 bp
ATGGCGGTTGTCACCGGAGATCGATACCTGGAGAAGCTAGTCCACTTCGTGGAGCAGCAGGCCGGTTCGTTGATCGACGGGAGCCTAGTGCTGAAGCTGAACCCGGCTGGATTCCACTACGTCAACTCCCGGCTCGAGGCCTTGCACGAGCTCGAGAGCCTCCTCGCCGGCGCTCCCGTCGATTACCTCCGCGCCTACGTCTCCGACCTTGGAGACCACCGCGCGCTCGAGCAGCTGAGGAGGATTCTCAGGCTCTTGACATCCCTCAAGGTCGTTTCGGTGCTGCCGCCTTCCGCCAGGGACCCTACGCCGCTGTCGTTTTGGCCGTTTGGGAGGCTTAAGGTTTTGGAGCTTCGAGGATGCGATTTATCCACCTCCGCCGCGAAAGGTTTGCTCGAGCTGCGGCACACCTTGGAGAAGATCATTTGCCATAATTCTACAGATGCTCTGCGGCATGTGTTTGCGAGTAGAATTGCAGAGATTAAGGACTCGCCGCAGTGGAACCGCCTGTCATTCGTTTCGTGTGCTTGTAATGGCTTGGTTCTCATGGATGAGTCTTTGCAACTTCTTCCTGCTGTCGAAACTCTCGACCTTAGCCGGAACAAGTTTGCAAAGGTTGATAATCTTCGCAAGTGTGTCAAATTGAAGCACCTAGACCTCGGTTTCAATCAGCTCAGAACGATTTCGTCATTTGGTCAGGTCACGTGCCGTTTACTTAAACTTGTTTTGAGGAACAATGCTATAACTACATTGCGTGGGATTGAGAATTTGAAGTCACTGGAAGGGCTTGATGCTTCCTACAATATTCTTTCCAATTTTTCGGAGTTAGAATACCTTTCTGGACTTCGATCTCTCGAAAACTTGTGGTTGGAAGGGAATCCATTATGTTGTGCCAGTTGGTATCGGTCACAAGCATTCAGCTATGTCACTAATCCAGAAAAATTGAAGTTAGATGATAAGGAGATCAGCACCAGAGAATTTTGGAAGAGGCAACTTATTATTGCTAGCAGGCACAAGCGACCTGCCAGCTTTGGGTTTTATTCTCCTGCAAAGTGCGATGATAAAGGAGATTCAATTATTAACAAAAAAAGGAAAAAGGTATCTCGTCTTGCATCAATTGTTAATGAAGAGGAGAACACATGTTCTGACCAGGACTCTGTGTCTTGTGACAATGAGATTCAAAGCAGAGAGGATGGTGTCATATCTGACGATGAAGCTGAAATTGTTGATTTGATGACTAGAGTTGAGCTAATGAAGAAAGAGCGCTCCGTTCTTTGGTTGCGGGAGTTCAAGGAATGGTTAGATCGTGCTTCTGAGAATGATGCAGACATCAGTAGATATAGCAGGGCTAGATTGCATCTTGAGAAGGAAAACTATATAAAAAACAAGGCAAGTTGGAGGCAGCTAGGTGAAAAATCAACATATGTTCAAGCATCAGGAGACGAGAGTAGTACAAATGTTTTAGAATCAATGGGGAATGCTGGTAGGGTCGGCCCAGTTGGCATGGATAGTAGAGATATCGGGGAGAACTTAAAAGCATATTCGTATGAAGGGACAAGTACTTTCAGTTCAGAGGCTAAAAGTTCCCATACTCAGACATTCACCACTGATGGAGGTCATAGGATGGTCCAAAATTTGAGTATGTCTGCATTGTCTGTCATTGATGATATTTCAGAGTCTTATTCATCATCTGCTAACCCAGGATCACCGCCCCATTATCAAAAGGACATTCTCCATCGTCGACATAACTTAGAGGAGGAAATTTTACAGTTATCCGCAGAGTCTTATTCAGTGGCATCATCTGATAGTAATACCAGCTCAAGTGAAGATGAAAATTGTGAATCTAGGCAGTCGGTTCCTGAGAACTTGTTGAATGAGAGTGCCGAAGAATACCCATCTAAAAATTGTTTCAAGTATTATGACATAAAACATGAGGTTGACAGATGTTCGGTCGGCGTACGTGCCAGTGACATTCCTGCTGATGCTCATGATGTTGAAATTACTAATTGCATTAATGAAGAGGGTGATTTGCCGGAGAGGAGGAAAGGAAGACAGAGGACTAAAAGAAGAGTTATCGCTCTAGTAGAAGATGATAACATGATTAGGCAAGCAGAACTGTCACCAAAATCAAATGGCAATCTAGATAATCATGTAGGGCAAATCGAAAATAAGCAAGAGAATAAATATTTCTATGGGAGTGATTTTCAAGAAGTTGTTGATAAGAAACAGATGTTGGCAAATAGAAGCAGTGCCTCAATAACCGAAATCTTATCTTCAGGAAGTGATGAATTTATTGAGAATTATTTCAATACAAATATCGCAGATTCACGAAATCACGAGATATGTAGGCAGTATTTGTGTTGCTGTTGTGTACTTGAGCGGGATTTTCTTTCCCGAGAAAGAGAAGTTGCTGTTGTACTTAGTAGTGAAGACAAGCTTTATGTACTGGCCATTGGTATTGCAGGAGATGGGTCAGGCACCATCTTAAAGTTACAGGGTCGTCACAGGGTTGAGGATATCAGAGAAGTTGTAGTTGGTATAGGGCTTCAGGTTGTGAGGGTGTATGTAGGAGGCAGAGCGACTTACCTGTTTAAAACTAGAAGCATTGAAAAATCAAGACAGTTACTTTCTACTTTAAAAGTTATCGATTCTTTTGCCCCAAATGGAGAACTTTGCTTAAGAAGTTTGGAGCAGGTTCAGGTGGAGTTATTTGAGAAACAAATATGTGGAGGTTCAAAAGTGAGCATATTCCAGTATTCCATGGTACAGTTTTGGTGTAGTTATAATGAAGGTGAATCATGGTTTTCAAGATCATTATTTGTGGCTGGAGGGCACGTATTTTTGTGCTTTGAAGACCTTATGCAGTTCAGCTCTTCATGCATGGATGCGTCTTTGCCCCCATATTTTTCACTTGACTTGTGTTGCTCTATTGCCGACATATCTGAATTGGTTGTCGATGTCAGAGAAAGTCGGTGTGTAACCATAGCTGTTGCGTGTGCAATGTCGGAGTTCTGCCCTCCAGGCTCAGCCGGAGCGGGCAATTCAGACTCAAGTGTAAATGATAAAAAGATTGCTCCAGGCTCCATGACTTGGAAGCTCCAGTGGTTTTCCGACGAGAGTCCGTTCAAGTTTGTGGCGTTGTTGAAGGCAATCCATGAAGGGATGACCGCTTCCCCCTTGCTTGTAAGATGTATATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4

Protein Analysis

1067

Amino Acids

119.65

Weight (kDa)

5.6

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 192 - 231 4.7e-07 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2784
Acc36I ACCTGC 2 cut(s) 1030, 2683
AccB1I GGYRCC 1 cut(s) 2477
AccB7I CCANNNNNTGG 2 cut(s) 49, 3090
AccBSI CCGCTC 2 cut(s) 2380, 3035
AccI GTMKAC 1 cut(s) 1741
AccII CGCG 4 cut(s) 192, 222, 224, 383
AclWI GGATC 1 cut(s) 1711
AcoI YGGCCR 2 cut(s) 323, 2448
AcsI RAATTY 5 cut(s) 766, 977, 1633, 1761, 2288
AcuI CTGAAG 3 cut(s) 113, 2259, 2531
AcvI CACGTG 1 cut(s) 705
AdeI CACNNNGTG 1 cut(s) 49
AfaI GTAC 7 cut(s) 1453, 1561, 1959, 2373, 2418, 2445, 2766
AfeI AGCGCT 1 cut(s) 1265
AfiI CCNNNNNNNGG 5 cut(s) 49, 136, 1032, 1275, 3090
AflII CTTAAG 2 cut(s) 338, 2677
AflIII ACRYGT 1 cut(s) 1139
AhdI GACNNNNNGTC 1 cut(s) 1159
AjnI CCWGG 6 cut(s) 27, 296, 1149, 1699, 3019, 3076
AjuI GAANNNNNNNTTGG 4 cut(s) 2216, 2248, 2517, 2549
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 3 cut(s) 205, 207, 1434
AlwI GGATC 1 cut(s) 1711
Ama87I CYCGRG 5 cut(s) 134, 152, 227, 395, 2394
Aor51HI AGCGCT 1 cut(s) 1265
AoxI GGCC 5 cut(s) 60, 138, 323, 1492, 2448
ApeKI GCWGC 5 cut(s) 55, 232, 283, 400, 1402
ApoI RAATTY 5 cut(s) 766, 977, 1633, 1761, 2288
AseI ATTAAT 1 cut(s) 2013
Asp700I GAANNNNTTC 2 cut(s) 682, 2303
AspLEI GCGC 5 cut(s) 173, 194, 224, 226, 1266
AspS9I GGNCC 3 cut(s) 301, 1493, 1627
AsuC2I CCSGG 2 cut(s) 104, 130
AsuHPI GGTGA 7 cut(s) 4, 1421, 1594, 1698, 2036, 2112, 2804
AsuNHI GCTAGC 1 cut(s) 1004
AvaI CYCGRG 5 cut(s) 134, 152, 227, 395, 2394
AvaII GGWCC 2 cut(s) 301, 1627
BaeGI GKGCMC 1 cut(s) 2835
BalI TGGCCA 1 cut(s) 2450
BanI GGYRCC 1 cut(s) 2477
BanII GRGCYC 1 cut(s) 153
BauI CACGAG 2 cut(s) 146, 2336
BbrPI CACGTG 1 cut(s) 705
BbsI GAAGAC 3 cut(s) 2056, 2436, 2859
Bbv12I GWGCWC 1 cut(s) 153
BbvCI CCTCAGC 1 cut(s) 236
BbvI GCAGC 5 cut(s) 67, 244, 270, 387, 1414
BccI CCATC 7 cut(s) 1187, 1604, 1618, 1743, 1903, 2462, 2489
BceAI ACGGC 2 cut(s) 310, 693
BcgI CGANNNNNNTGC 4 cut(s) 373, 407, 2636, 2670
BciT130I CCWGG 6 cut(s) 29, 298, 1151, 1701, 3021, 3078
BcnI CCSGG 2 cut(s) 104, 130
BcoDI GTCTC 3 cut(s) 205, 207, 1434
BfmI CTRYAG 1 cut(s) 438
BfoI RGCGCY 2 cut(s) 174, 1267
BfrI CTTAAG 2 cut(s) 338, 2677
BfuAI ACCTGC 2 cut(s) 1030, 2683
BglI GCCNNNNNGGC 2 cut(s) 1500, 3021
BlpI GCTNAGC 1 cut(s) 3025
BmcAI AGTACT 1 cut(s) 1561
Bme1390I CCNGG 8 cut(s) 29, 104, 130, 298, 1151, 1701, 3021, 3078
Bme18I GGWCC 2 cut(s) 301, 1627
BmeRI GACNNNNNGTC 1 cut(s) 1159
BmeT110I CYCGRG 5 cut(s) 134, 152, 227, 395, 2394
BmgT120I GGNCC 3 cut(s) 301, 1493, 1627
BmiI GGNNCC 7 cut(s) 83, 302, 303, 503, 1857, 2479, 3082
BmrFI CCNGG 8 cut(s) 29, 104, 130, 298, 1151, 1701, 3021, 3078
BmrI ACTGGG 1 cut(s) 1490
BmtI GCTAGC 1 cut(s) 1008
BmuI ACTGGG 1 cut(s) 1490
BoxI GACNNNNGTC 1 cut(s) 3117
BpiI GAAGAC 3 cut(s) 2056, 2436, 2859
BpmI CTGGAG 5 cut(s) 50, 2847, 3003, 3060, 3083
Bpu10I CCTNAGC 2 cut(s) 236, 593
Bpu1102I GCTNAGC 1 cut(s) 3025
BpuEI CTTGAG 5 cut(s) 251, 1382, 1804, 2396, 3034
BpuMI CCSGG 2 cut(s) 104, 130
Bsa29I ATCGAT 2 cut(s) 22, 2646
BsaAI YACGTR 3 cut(s) 705, 1961, 2836
BsaBI GATNNNNATC 1 cut(s) 3193
BsaI GGTCTC 1 cut(s) 207
BsaJI CCNNGG 6 cut(s) 208, 297, 411, 658, 1699, 2760
BsaWI WCCGGW 1 cut(s) 14
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 2820, 2850
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 136, 1032, 1275, 3090
Bse118I RCCGGY 2 cut(s) 62, 167
Bse1I ACTGG 7 cut(s) 783, 894, 1496, 1965, 2451, 2752, 3100
Bse3DI GCAATG 2 cut(s) 749, 3006
Bse8I GATNNNNATC 1 cut(s) 3193
BseBI CCWGG 6 cut(s) 29, 298, 1151, 1701, 3021, 3078
BseCI ATCGAT 2 cut(s) 22, 2646
BseDI CCNNGG 6 cut(s) 208, 297, 411, 658, 1699, 2760
BseGI GGATG 7 cut(s) 260, 365, 556, 1198, 1629, 2890, 3168
BseJI GATNNNNATC 1 cut(s) 3193
BseLI CCNNNNNNNGG 5 cut(s) 49, 136, 1032, 1275, 3090
BseMI GCAATG 2 cut(s) 749, 3006
BseMII CTCAG 7 cut(s) 227, 262, 688, 1305, 1607, 1851, 3039
BseNI ACTGG 7 cut(s) 783, 894, 1496, 1965, 2451, 2752, 3100
BsePI GCGCGC 1 cut(s) 222
BseRI GAGGAG 5 cut(s) 152, 253, 1145, 1769, 2053
BseSI GKGCMC 1 cut(s) 2835
BseXI GCAGC 5 cut(s) 67, 244, 270, 387, 1414
Bsh1236I CGCG 4 cut(s) 192, 222, 224, 383
Bsh1285I CGRYCG 1 cut(s) 1953
BshFI GGCC 5 cut(s) 62, 140, 325, 1494, 2450
BshNI GGYRCC 1 cut(s) 2477
BshVI ATCGAT 2 cut(s) 22, 2646
BsiEI CGRYCG 1 cut(s) 1953
BsiHKAI GWGCWC 1 cut(s) 153
BsiHKCI CYCGRG 5 cut(s) 134, 152, 227, 395, 2394
BsiSI CCGG 8 cut(s) 15, 63, 104, 130, 168, 598, 2033, 3030
BsiWI CGTACG 1 cut(s) 1957
BslFI GGGAC 2 cut(s) 314, 1567
BslI CCNNNNNNNGG 5 cut(s) 49, 136, 1032, 1275, 3090
BsmAI GTCTC 3 cut(s) 205, 207, 1434
BsmBI CGTCTC 2 cut(s) 205, 1434
BsmFI GGGAC 2 cut(s) 314, 1567
BsmI GAATGC 2 cut(s) 914, 1483
BsnI GGCC 5 cut(s) 62, 140, 325, 1494, 2450
Bso31I GGTCTC 1 cut(s) 207
BsoBI CYCGRG 5 cut(s) 134, 152, 227, 395, 2394
Bsp1286I GDGCHC 2 cut(s) 153, 2835
Bsp143I GATC 8 cut(s) 19, 72, 420, 848, 963, 1303, 1703, 2810
Bsp1720I GCTNAGC 1 cut(s) 3025
Bsp19I CCATGG 1 cut(s) 2760
BspANI GGCC 5 cut(s) 62, 140, 325, 1494, 2450
BspCNI CTCAG 7 cut(s) 228, 261, 687, 1306, 1606, 1852, 3038
BspDI ATCGAT 2 cut(s) 22, 2646
BspFNI CGCG 4 cut(s) 192, 222, 224, 383
BspHI TCATGA 1 cut(s) 1987
BspLI GGNNCC 7 cut(s) 83, 302, 303, 503, 1857, 2479, 3082
BspMI ACCTGC 2 cut(s) 1030, 2683
BspOI GCTAGC 1 cut(s) 1008
BspPI GGATC 1 cut(s) 1711
BspQI GCTCTTC 1 cut(s) 2878
BspT107I GGYRCC 1 cut(s) 2477
BspTI CTTAAG 2 cut(s) 338, 2677
BspTNI GGTCTC 1 cut(s) 207
BsrBI CCGCTC 2 cut(s) 2380, 3035
BsrDI GCAATG 2 cut(s) 749, 3006
BsrFI RCCGGY 2 cut(s) 62, 167
BsrI ACTGG 7 cut(s) 783, 894, 1496, 1965, 2451, 2752, 3100
BssAI RCCGGY 2 cut(s) 62, 167
BssECI CCNNGG 6 cut(s) 208, 297, 411, 658, 1699, 2760
BssHII GCGCGC 1 cut(s) 222
BssMI GATC 8 cut(s) 19, 72, 420, 848, 963, 1303, 1703, 2810
BssSI CACGAG 2 cut(s) 146, 2336
BssT1I CCWWGG 3 cut(s) 208, 411, 2760
Bst2BI CACGAG 2 cut(s) 146, 2336
Bst2UI CCWGG 6 cut(s) 29, 298, 1151, 1701, 3021, 3078
Bst4CI ACNGT 4 cut(s) 1770, 2118, 2622, 2769
Bst6I CTCTTC 5 cut(s) 980, 1122, 2013, 2062, 2878
BstAFI CTTAAG 2 cut(s) 338, 2677
BstBAI YACGTR 3 cut(s) 705, 1961, 2836
BstC8I GCNNGC 8 cut(s) 60, 169, 224, 1006, 1010, 1027, 2109, 3037
BstDSI CCRYGG 1 cut(s) 2760
BstF5I GGATG 7 cut(s) 260, 365, 556, 1198, 1629, 2890, 3168
BstFNI CGCG 4 cut(s) 192, 222, 224, 383
BstH2I RGCGCY 2 cut(s) 174, 1267
BstHHI GCGC 5 cut(s) 173, 194, 224, 226, 1266
BstKTI GATC 8 cut(s) 22, 75, 423, 851, 966, 1306, 1706, 2813
BstMAI GTCTC 3 cut(s) 205, 207, 1434
BstMBI GATC 8 cut(s) 19, 72, 420, 848, 963, 1303, 1703, 2810
BstMCI CGRYCG 1 cut(s) 1953
BstMWI GCNNNNNNNGC 5 cut(s) 232, 400, 1500, 2877, 3021
BstNI CCWGG 6 cut(s) 29, 298, 1151, 1701, 3021, 3078
BstNSI RCATGY 2 cut(s) 458, 1143
BstPAI GACNNNNGTC 1 cut(s) 3117
BstSCI CCNGG 8 cut(s) 27, 102, 128, 296, 1149, 1699, 3019, 3076
BstSFI CTRYAG 1 cut(s) 438
BstSLI GKGCMC 1 cut(s) 2835
BstUI CGCG 4 cut(s) 192, 222, 224, 383
BstV1I GCAGC 5 cut(s) 67, 244, 270, 387, 1414
BstV2I GAAGAC 3 cut(s) 2056, 2436, 2859
Bsu15I ATCGAT 2 cut(s) 22, 2646
BsuRI GGCC 5 cut(s) 62, 140, 325, 1494, 2450
BsuTUI ATCGAT 2 cut(s) 22, 2646
BtgI CCRYGG 1 cut(s) 2760
BtgZI GCGATG 1 cut(s) 1071
BtsCI GGATG 7 cut(s) 260, 365, 556, 1198, 1629, 2890, 3168
BtsI GCAGTG 2 cut(s) 503, 2254
BtsIMutI CAGTG 7 cut(s) 503, 776, 1605, 1797, 1972, 2254, 3107
BveI ACCTGC 2 cut(s) 1030, 2683
Cac8I GCNNGC 8 cut(s) 60, 169, 224, 1006, 1010, 1027, 2109, 3037
CciI TCATGA 1 cut(s) 1987
CfoI GCGC 5 cut(s) 173, 194, 224, 226, 1266
Cfr10I RCCGGY 2 cut(s) 62, 167
Cfr13I GGNCC 3 cut(s) 301, 1493, 1627
ClaI ATCGAT 2 cut(s) 22, 2646
CseI GACGC 1 cut(s) 2877
Csp6I GTAC 7 cut(s) 1452, 1560, 1958, 2372, 2417, 2444, 2765
CviQI GTAC 7 cut(s) 1452, 1560, 1958, 2372, 2417, 2444, 2765
DpnI GATC 8 cut(s) 21, 74, 422, 850, 965, 1305, 1705, 2812
DpnII GATC 8 cut(s) 19, 72, 420, 848, 963, 1303, 1703, 2810
DraI TTTAAA 2 cut(s) 2593, 2637
DraIII CACNNNGTG 1 cut(s) 49
DriI GACNNNNNGTC 1 cut(s) 1159
EaeI YGGCCR 2 cut(s) 323, 2448
Eam1104I CTCTTC 5 cut(s) 980, 1122, 2013, 2062, 2878
Eam1105I GACNNNNNGTC 1 cut(s) 1159
EarI CTCTTC 5 cut(s) 980, 1122, 2013, 2062, 2878
EciI GGCGGA 2 cut(s) 283, 367
Ecl136II GAGCTC 1 cut(s) 151
Eco130I CCWWGG 3 cut(s) 208, 411, 2760
Eco147I AGGCCT 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 153
Eco31I GGTCTC 1 cut(s) 207
Eco32I GATATC 2 cut(s) 1519, 2518
Eco47I GGWCC 2 cut(s) 301, 1627
Eco47III AGCGCT 1 cut(s) 1265
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 3 cut(s) 113, 2259, 2531
Eco72I CACGTG 1 cut(s) 705
Eco88I CYCGRG 5 cut(s) 134, 152, 227, 395, 2394
EcoICRI GAGCTC 1 cut(s) 151
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 6 cut(s) 27, 296, 1149, 1699, 3019, 3076
EcoRV GATATC 2 cut(s) 1519, 2518
EcoT14I CCWWGG 3 cut(s) 208, 411, 2760
EcoT22I ATGCAT 1 cut(s) 2882
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 3 cut(s) 208, 411, 2760
Esp3I CGTCTC 2 cut(s) 205, 1434
FalI AAGNNNNNCTT 2 cut(s) 2421, 2453
FaqI GGGAC 2 cut(s) 314, 1567
FauI CCCGC 3 cut(s) 1275, 2373, 3028
FauNDI CATATG 1 cut(s) 1423
FblI GTMKAC 1 cut(s) 1741
FokI GGATG 7 cut(s) 247, 372, 563, 1205, 1636, 2897, 3175
FriOI GRGCYC 1 cut(s) 153
GlaI GCGC 5 cut(s) 172, 193, 223, 225, 1265
GsuI CTGGAG 5 cut(s) 50, 2847, 3003, 3060, 3083
HaeII RGCGCY 2 cut(s) 174, 1267
HaeIII GGCC 5 cut(s) 62, 140, 325, 1494, 2450
HapII CCGG 8 cut(s) 15, 63, 104, 130, 168, 598, 2033, 3030
HgaI GACGC 1 cut(s) 2877
HhaI GCGC 5 cut(s) 173, 194, 224, 226, 1266
Hin6I GCGC 5 cut(s) 171, 192, 222, 224, 1264
HinP1I GCGC 5 cut(s) 171, 192, 222, 224, 1264
HincII GTYRAC 3 cut(s) 124, 1742, 1939
HindII GTYRAC 3 cut(s) 124, 1742, 1939
HindIII AAGCTT 1 cut(s) 2435
HpaII CCGG 8 cut(s) 15, 63, 104, 130, 168, 598, 2033, 3030
HphI GGTGA 7 cut(s) 4, 1421, 1594, 1698, 2036, 2112, 2804
Hpy166II GTNNAC 6 cut(s) 43, 124, 713, 1742, 1939, 2372
Hpy8I GTNNAC 6 cut(s) 43, 124, 713, 1742, 1939, 2372
Hpy99I CGWCG 4 cut(s) 80, 182, 1743, 3116
HpyAV CCTTC 8 cut(s) 300, 776, 866, 1360, 1544, 2783, 3139, 3152
HpyCH4III ACNGT 4 cut(s) 1770, 2118, 2622, 2769
HpyCH4IV ACGT 5 cut(s) 120, 198, 704, 1960, 2835
HpyF10VI GCNNNNNNNGC 5 cut(s) 232, 400, 1500, 2877, 3021
HpySE526I ACGT 5 cut(s) 120, 198, 704, 1960, 2835
HspAI GCGC 5 cut(s) 171, 192, 222, 224, 1264
KflI GGGWCCC 1 cut(s) 301
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 8 cut(s) 19, 72, 420, 848, 963, 1303, 1703, 2810
LguI GCTCTTC 1 cut(s) 2878
Lsp1109I GCAGC 5 cut(s) 67, 244, 270, 387, 1414
MaeII ACGT 5 cut(s) 120, 198, 704, 1960, 2835
MalI GATC 8 cut(s) 21, 74, 422, 850, 965, 1305, 1705, 2812
MbiI CCGCTC 2 cut(s) 2380, 3035
MboI GATC 8 cut(s) 19, 72, 420, 848, 963, 1303, 1703, 2810
MfeI CAATTG 1 cut(s) 1116
MhlI GDGCHC 2 cut(s) 153, 2835
MlsI TGGCCA 1 cut(s) 2450
MluNI TGGCCA 1 cut(s) 2450
MlyI GAGTC 7 cut(s) 482, 563, 1148, 1685, 1790, 3041, 3127
MmeI TCCRAC 5 cut(s) 228, 849, 1376, 2985, 3135
Mox20I TGGCCA 1 cut(s) 2450
Mph1103I ATGCAT 1 cut(s) 2882
MreI CGCCGGCG 1 cut(s) 167
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 2 cut(s) 682, 2303
MscI TGGCCA 1 cut(s) 2450
Msp20I TGGCCA 1 cut(s) 2450
MspA1I CMGCKG 2 cut(s) 235, 313
MspCI CTTAAG 2 cut(s) 338, 2677
MspI CCGG 8 cut(s) 15, 63, 104, 130, 168, 598, 2033, 3030
MspR9I CCNGG 8 cut(s) 29, 104, 130, 298, 1151, 1701, 3021, 3078
MunI CAATTG 1 cut(s) 1116
Mva1269I GAATGC 2 cut(s) 914, 1483
MvaI CCWGG 6 cut(s) 29, 298, 1151, 1701, 3021, 3078
MvnI CGCG 4 cut(s) 192, 222, 224, 383
MwoI GCNNNNNNNGC 5 cut(s) 232, 400, 1500, 2877, 3021
NaeI GCCGGC 1 cut(s) 169
NciI CCSGG 2 cut(s) 104, 130
NcoI CCATGG 1 cut(s) 2760
NdeI CATATG 1 cut(s) 1423
NdeII GATC 8 cut(s) 19, 72, 420, 848, 963, 1303, 1703, 2810
NgoMIV GCCGGC 1 cut(s) 167
NheI GCTAGC 1 cut(s) 1004
NlaIV GGNNCC 7 cut(s) 83, 302, 303, 503, 1857, 2479, 3082
NmuCI GTSAC 9 cut(s) 10, 700, 774, 906, 925, 1166, 1967, 2118, 2501
NsiI ATGCAT 1 cut(s) 2882
NspI RCATGY 2 cut(s) 458, 1143
PaeR7I CTCGAG 4 cut(s) 134, 152, 227, 395
PagI TCATGA 1 cut(s) 1987
PauI GCGCGC 1 cut(s) 222
PceI AGGCCT 1 cut(s) 140
PciI ACATGT 1 cut(s) 1139
PciSI GCTCTTC 1 cut(s) 2878
PcsI WCGNNNNNNNCGW 3 cut(s) 314, 323, 585
PctI GAATGC 2 cut(s) 914, 1483
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 2 cut(s) 682, 2303
Pfl23II CGTACG 1 cut(s) 1957
PflMI CCANNNNNTGG 2 cut(s) 49, 3090
PleI GAGTC 7 cut(s) 482, 562, 1148, 1684, 1789, 3041, 3126
PmaCI CACGTG 1 cut(s) 705
PmlI CACGTG 1 cut(s) 705
PpsI GAGTC 7 cut(s) 482, 562, 1148, 1684, 1789, 3041, 3126
Ppu21I YACGTR 3 cut(s) 705, 1961, 2836
PpuMI RGGWCCY 1 cut(s) 301
PscI ACATGT 1 cut(s) 1139
PshAI GACNNNNGTC 1 cut(s) 3117
PshBI ATTAAT 1 cut(s) 2013
PsiI TTATAA 1 cut(s) 2784
Psp124BI GAGCTC 1 cut(s) 153
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 6 cut(s) 27, 296, 1149, 1699, 3019, 3076
PspCI CACGTG 1 cut(s) 705
PspGI CCWGG 6 cut(s) 27, 296, 1149, 1699, 3019, 3076
PspLI CGTACG 1 cut(s) 1957
PspN4I GGNNCC 7 cut(s) 83, 302, 303, 503, 1857, 2479, 3082
PspPI GGNCC 3 cut(s) 301, 1493, 1627
PspPPI RGGWCCY 1 cut(s) 301
PspXI VCTCGAGB 3 cut(s) 134, 227, 395
PsrI GAACNNNNNNTAC 2 cut(s) 1552, 1584
PteI GCGCGC 1 cut(s) 222
PvuII CAGCTG 1 cut(s) 235
RsaI GTAC 7 cut(s) 1453, 1561, 1959, 2373, 2418, 2445, 2766
RsaNI GTAC 7 cut(s) 1452, 1560, 1958, 2372, 2417, 2444, 2765
SacI GAGCTC 1 cut(s) 153
SalI GTCGAC 1 cut(s) 1740
SapI GCTCTTC 1 cut(s) 2878
Sau3AI GATC 8 cut(s) 19, 72, 420, 848, 963, 1303, 1703, 2810
Sau96I GGNCC 3 cut(s) 301, 1493, 1627
ScaI AGTACT 1 cut(s) 1561
SchI GAGTC 7 cut(s) 482, 563, 1148, 1685, 1790, 3041, 3127
ScrFI CCNGG 8 cut(s) 29, 104, 130, 298, 1151, 1701, 3021, 3078
SduI GDGCHC 2 cut(s) 153, 2835
SfcI CTRYAG 1 cut(s) 438
Sfr274I CTCGAG 4 cut(s) 134, 152, 227, 395
SgrAI CRCCGGYG 1 cut(s) 167
SinI GGWCC 2 cut(s) 301, 1627
SlaI CTCGAG 4 cut(s) 134, 152, 227, 395
SseBI AGGCCT 1 cut(s) 140
SspI AATATT 2 cut(s) 805, 2186
SstI GAGCTC 1 cut(s) 153
StuI AGGCCT 1 cut(s) 140
StyD4I CCNGG 8 cut(s) 27, 102, 128, 296, 1149, 1699, 3019, 3076
StyI CCWWGG 3 cut(s) 208, 411, 2760
TaaI ACNGT 4 cut(s) 1770, 2118, 2622, 2769
TaiI ACGT 5 cut(s) 123, 201, 707, 1963, 2838
TaqII GACCGA 2 cut(s) 894, 1939
TatI WGTACW 5 cut(s) 1451, 1559, 2371, 2416, 2443
TauI GCSGC 6 cut(s) 289, 313, 383, 406, 454, 496
TscAI CASTG 7 cut(s) 503, 783, 1612, 1797, 1972, 2254, 3107
TseFI GTSAC 9 cut(s) 10, 700, 774, 906, 925, 1166, 1967, 2118, 2501
TseI GCWGC 5 cut(s) 55, 232, 283, 400, 1402
Tsp45I GTSAC 9 cut(s) 10, 700, 774, 906, 925, 1166, 1967, 2118, 2501
TspGWI ACGGA 2 cut(s) 1258, 3111
TspRI CASTG 7 cut(s) 503, 783, 1612, 1797, 1972, 2254, 3107
Van91I CCANNNNNTGG 2 cut(s) 49, 3090
Vha464I CTTAAG 2 cut(s) 338, 2677
VpaK11BI GGWCC 2 cut(s) 301, 1627
VspI ATTAAT 1 cut(s) 2013
XapI RAATTY 5 cut(s) 766, 977, 1633, 1761, 2288
XbaI TCTAGA 1 cut(s) 2140
XceI RCATGY 2 cut(s) 458, 1143
XhoI CTCGAG 4 cut(s) 134, 152, 227, 395
XmiI GTMKAC 1 cut(s) 1741
XmnI GAANNNNTTC 2 cut(s) 682, 2303
ZrmI AGTACT 1 cut(s) 1561
Zsp2I ATGCAT 1 cut(s) 2882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.