pycom06g20600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0007754
Physical Location & Seq
Forward (+)
23558276 .. 23558768
493 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g20600.1

Sequence Viewer

Length: 375 bp
ATGAAATACACAGGACCTAAACCCTCTATATACCCGCGTACTCCTCATCTGCCCCTTAGAATTTTACATACATACAAAACATATAGATATACATCAAACAAAATTGGACAAGTATATACTTCTGGGAGGAAGAGGAAGAAGAAGATGGAGAACAGAAACAGCAACAACAGCAGAAAGATGGAGGAGGGATTTGAGGAAGTGGGCTTTCCAGTTCACAGCCAAGTGAGGAAGATCAAGCAAGAATGCGACAAAACCATCGACTGGTTGCCGGGAAATCCGGAGATGAGAACACCCGTTTTTAAAGAGATCACCAGGCAGGTCTCTCGATCTCCTCTCGGAAATTCTTGCAGGCCGATCTCCGTCGGCGACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

14.44

Weight (kDa)

10.14

Isoelectric Point (pI)

54.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 307
AccB7I CCANNNNNTGG 1 cut(s) 261
AccII CGCG 1 cut(s) 37
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 1 cut(s) 35
AcsI RAATTY 2 cut(s) 60, 340
AfaI GTAC 1 cut(s) 40
AfiI CCNNNNNNNGG 1 cut(s) 261
AjnI CCWGG 1 cut(s) 311
Alw26I GTCTC 1 cut(s) 325
Aor13HI TCCGGA 1 cut(s) 277
AoxI GGCC 1 cut(s) 350
ApoI RAATTY 2 cut(s) 60, 340
AspS9I GGNCC 1 cut(s) 14
AsuC2I CCSGG 1 cut(s) 270
AsuHPI GGTGA 1 cut(s) 301
AvaII GGWCC 1 cut(s) 14
BccI CCATC 3 cut(s) 139, 172, 263
BcgI CGANNNNNNTGC 2 cut(s) 305, 339
BciT130I CCWGG 1 cut(s) 313
BcnI CCSGG 1 cut(s) 270
BcoDI GTCTC 1 cut(s) 325
BfuAI ACCTGC 1 cut(s) 307
Bme1390I CCNGG 2 cut(s) 270, 313
Bme18I GGWCC 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 14
BmrFI CCNGG 2 cut(s) 270, 313
BpuMI CCSGG 1 cut(s) 270
BsaBI GATNNNNATC 1 cut(s) 91
BsaI GGTCTC 1 cut(s) 325
BsaWI WCCGGW 1 cut(s) 277
Bsc4I CCNNNNNNNGG 1 cut(s) 261
Bse1I ACTGG 2 cut(s) 209, 266
Bse8I GATNNNNATC 1 cut(s) 91
BseAI TCCGGA 1 cut(s) 277
BseBI CCWGG 1 cut(s) 313
BseJI GATNNNNATC 1 cut(s) 91
BseLI CCNNNNNNNGG 1 cut(s) 261
BseNI ACTGG 2 cut(s) 209, 266
BseRI GAGGAG 3 cut(s) 33, 197, 321
Bsh1236I CGCG 1 cut(s) 37
BshFI GGCC 1 cut(s) 352
BsiSI CCGG 2 cut(s) 269, 278
BslI CCNNNNNNNGG 1 cut(s) 261
BsmAI GTCTC 1 cut(s) 325
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 1 cut(s) 352
Bso31I GGTCTC 1 cut(s) 325
Bsp13I TCCGGA 1 cut(s) 277
Bsp143I GATC 4 cut(s) 231, 306, 326, 354
BspACI CCGC 1 cut(s) 35
BspANI GGCC 1 cut(s) 352
BspEI TCCGGA 1 cut(s) 277
BspFNI CGCG 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 307
BspTNI GGTCTC 1 cut(s) 325
BsrI ACTGG 2 cut(s) 209, 266
BssMI GATC 4 cut(s) 231, 306, 326, 354
Bst2UI CCWGG 1 cut(s) 313
Bst6I CTCTTC 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 350
BstDEI CTNAG 1 cut(s) 56
BstFNI CGCG 1 cut(s) 37
BstKTI GATC 4 cut(s) 234, 309, 329, 357
BstMAI GTCTC 1 cut(s) 325
BstMBI GATC 4 cut(s) 231, 306, 326, 354
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstNI CCWGG 1 cut(s) 313
BstSCI CCNGG 2 cut(s) 268, 311
BstUI CGCG 1 cut(s) 37
BsuRI GGCC 1 cut(s) 352
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 1 cut(s) 350
Cfr13I GGNCC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 39
CviJI RGCY 3 cut(s) 204, 219, 352
CviKI_1 RGCY 3 cut(s) 204, 219, 352
CviQI GTAC 1 cut(s) 39
DdeI CTNAG 1 cut(s) 56
DpnI GATC 4 cut(s) 233, 308, 328, 356
DpnII GATC 4 cut(s) 231, 306, 326, 354
DraI TTTAAA 1 cut(s) 301
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Eco31I GGTCTC 1 cut(s) 325
Eco47I GGWCC 1 cut(s) 14
EcoO109I RGGNCCY 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 311
FaiI YATR 9 cut(s) 29, 31, 69, 73, 82, 84, 90, 115, 117
FauI CCCGC 1 cut(s) 42
HaeIII GGCC 1 cut(s) 352
HapII CCGG 2 cut(s) 269, 278
HinfI GANTC 1 cut(s) 368
HpaII CCGG 2 cut(s) 269, 278
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 1 cut(s) 338
Hpy188III TCNNGA 2 cut(s) 278, 324
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 1 cut(s) 365
HpyCH4V TGCA 1 cut(s) 348
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpyF3I CTNAG 1 cut(s) 56
Kpn2I TCCGGA 1 cut(s) 277
Kzo9I GATC 4 cut(s) 231, 306, 326, 354
LpnPI CCDG 9 cut(s) 108, 222, 247, 282, 291, 298, 302, 325, 334
MalI GATC 4 cut(s) 233, 308, 328, 356
MboI GATC 4 cut(s) 231, 306, 326, 354
MboII GAAGA 5 cut(s) 142, 148, 151, 154, 241
MluCI AATT 3 cut(s) 60, 102, 340
MlyI GAGTC 1 cut(s) 362
MnlI CCTC 9 cut(s) 34, 54, 120, 126, 175, 178, 187, 219, 342
MroI TCCGGA 1 cut(s) 277
MseI TTAA 2 cut(s) 300, 373
MspI CCGG 2 cut(s) 269, 278
MspR9I CCNGG 2 cut(s) 270, 313
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 1 cut(s) 313
MvnI CGCG 1 cut(s) 37
MwoI GCNNNNNNNGC 1 cut(s) 168
NciI CCSGG 1 cut(s) 270
NdeII GATC 4 cut(s) 231, 306, 326, 354
PctI GAATGC 1 cut(s) 248
PflMI CCANNNNNTGG 1 cut(s) 261
PleI GAGTC 1 cut(s) 362
PpsI GAGTC 1 cut(s) 362
PpuMI RGGWCCY 1 cut(s) 14
Psp5II RGGWCCY 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 311
PspGI CCWGG 1 cut(s) 311
PspPI GGNCC 1 cut(s) 14
PspPPI RGGWCCY 1 cut(s) 14
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
SaqAI TTAA 2 cut(s) 300, 373
Sau3AI GATC 4 cut(s) 231, 306, 326, 354
Sau96I GGNCC 1 cut(s) 14
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 2 cut(s) 270, 313
SetI ASST 2 cut(s) 19, 321
SinI GGWCC 1 cut(s) 14
Sse9I AATT 3 cut(s) 60, 102, 340
SsiI CCGC 1 cut(s) 35
StyD4I CCNGG 2 cut(s) 268, 311
TaqI TCGA 2 cut(s) 258, 325
TasI AATT 3 cut(s) 60, 102, 340
Tru1I TTAA 2 cut(s) 300, 373
Tru9I TTAA 2 cut(s) 300, 373
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 349
Van91I CCANNNNNTGG 1 cut(s) 261
VpaK11BI GGWCC 1 cut(s) 14
XapI RAATTY 2 cut(s) 60, 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.