pycom06g21440

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0010387
Physical Location & Seq
Reverse (-)
24087030 .. 24090018
2989 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1092 bp
ATGAGGCTGGCATGTTTTGAGGAATGCCAACCGTTTTTCCTTGGAATTAGCCTCTACCTTCATTCCATACTCTCTCTCTCTCTCCGTGGCCTTGGGCCTTGCCTTCCTTCGCTCTCAAGTCTTAACGCCTTGCCTGCCTCCGTTGTTGCTGCTGCTGCTGCGTATGTGTTATTGTGTTGTTGTTTCCGGTCCCGTATCATCCAAACCAACAAACTAGCGAAGGTAGGCGCACAAGCATACACATTCACACTAGCAACAATGAACAACAAGGCCTCCGTTTCCAAAGACCTCAACGCCAAACACCTCAAGATTTTAGAAGGCCTTCTTAAGCTGCCACAAAACAGGGAATGTGCTGATTGCCGGAGCAAAGCCCCGCGATGGGCTAGTGTCAACCTGGGAATATTTATTTGCATGCAGTGCTCAGGAATTCATCGGAGTCTTGGAGTGCATATATCAAAGGTACGGTCTACAACTTTGGATACATGGCTGCCTGAACAGGTTGCTTTTATGGAATCCATGGGTAATGAAAAGGCAAACAGTTATTGGGAAAAAGAATTGCCACCAAATTTTGACAGAAGTGGGACTGAGAATTTTATTCGTGCCAAGTATGAGGGGAAAATGTGGGGTTCGAAGAATGCAAAACAACCAGCTCCTAAACCAGTAGATATGAGCATCAGTTCTAACAGTTTGGTAAAGGGCAGAGCCACATGTGGAGTTCCTAAGAAAACAAGGAGACATTCTCTTGAGGAGGCAATGCTTTTTACAAACCTGGCAGAAGCTGCTCCTACAGTGGCAAGATCCCGTGGGGGTTCATTAGATTTGAATCATAAGTTTACTACGTCACCTCCAGCAAAGGGGCCACCACCAACGATGGAATGTGATACAGTCACAAAAAAGATCAACGGTACAACAGACCTATTCAGCTTGCTTTATGTTCATGATGCAAAGCAGAAGTACGCCACTCCTCCGCTCTGGCAAACTTTTGATTGTAAGAAAACTATAGTAACATCTTTCTTTTTGTTACTTGTTTACTATTTTGCAGTTGGGAAGGAAGTAGTAAAATTGATGAAGGGAAGATGGAAAATAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

364

Amino Acids

40.18

Weight (kDa)

9.56

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 104 - 211 1.6e-41 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 970
AccI GTMKAC 1 cut(s) 467
AccII CGCG 1 cut(s) 376
AciI CCGC 2 cut(s) 374, 968
AclWI GGATC 1 cut(s) 792
AcsI RAATTY 3 cut(s) 426, 565, 589
AfaI GTAC 3 cut(s) 462, 907, 956
AfiI CCNNNNNNNGG 3 cut(s) 378, 379, 854
AflII CTTAAG 1 cut(s) 326
AflIII ACRYGT 1 cut(s) 707
AgsI TTSAA 1 cut(s) 823
AjnI CCWGG 2 cut(s) 393, 768
AluBI AGCT 4 cut(s) 331, 650, 779, 924
AluI AGCT 4 cut(s) 331, 650, 779, 924
Alw21I GWGCWC 1 cut(s) 422
Alw26I GTCTC 1 cut(s) 727
AlwI GGATC 1 cut(s) 792
AlwNI CAGNNNCTG 1 cut(s) 779
AoxI GGCC 5 cut(s) 88, 95, 270, 319, 857
ApeKI GCWGC 7 cut(s) 149, 152, 155, 158, 331, 487, 779
ApoI RAATTY 3 cut(s) 426, 565, 589
ArsI GACNNNNNNTTYG 2 cut(s) 449, 481
Asp700I GAANNNNTTC 1 cut(s) 321
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 3 cut(s) 95, 189, 857
AsuHPI GGTGA 1 cut(s) 834
AsuII TTCGAA 1 cut(s) 629
AvaII GGWCC 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 422
BbvI GCAGC 7 cut(s) 136, 139, 142, 145, 318, 474, 766
BccI CCATC 3 cut(s) 372, 865, 1071
BciT130I CCWGG 2 cut(s) 395, 770
BciVI GTATCC 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 727
BfaI CTAG 3 cut(s) 215, 251, 384
BfmI CTRYAG 2 cut(s) 786, 999
BfrI CTTAAG 1 cut(s) 326
BfuI GTATCC 1 cut(s) 472
BisI GCNGC 7 cut(s) 150, 153, 156, 159, 332, 488, 780
BlsI GCNGC 7 cut(s) 151, 154, 157, 160, 333, 489, 781
Bme1390I CCNGG 2 cut(s) 395, 770
Bme18I GGWCC 1 cut(s) 189
BmgT120I GGNCC 3 cut(s) 95, 189, 857
BmiI GGNNCC 2 cut(s) 191, 858
BmrFI CCNGG 2 cut(s) 395, 770
BmsI GCATC 2 cut(s) 681, 931
BplI GAGNNNNNCTC 2 cut(s) 724, 756
BpmI CTGGAG 1 cut(s) 831
Bpu10I CCTNAGC 1 cut(s) 421
Bpu14I TTCGAA 1 cut(s) 629
BpuEI CTTGAG 3 cut(s) 100, 290, 764
BsaBI GATNNNNATC 1 cut(s) 822
BsaJI CCNNGG 6 cut(s) 40, 85, 91, 394, 516, 802
BsaWI WCCGGW 1 cut(s) 186
BsaXI ACNNNNNCTCC 4 cut(s) 257, 287, 829, 859
Bsc4I CCNNNNNNNGG 3 cut(s) 378, 379, 854
Bse1I ACTGG 1 cut(s) 659
Bse3DI GCAATG 1 cut(s) 759
Bse8I GATNNNNATC 1 cut(s) 822
BseBI CCWGG 2 cut(s) 395, 770
BseDI CCNNGG 6 cut(s) 40, 85, 91, 394, 516, 802
BseGI GGATG 1 cut(s) 198
BseJI GATNNNNATC 1 cut(s) 822
BseLI CCNNNNNNNGG 3 cut(s) 378, 379, 854
BseMI GCAATG 1 cut(s) 759
BseMII CTCAG 2 cut(s) 435, 576
BseNI ACTGG 1 cut(s) 659
BseRI GAGGAG 2 cut(s) 761, 954
BseXI GCAGC 7 cut(s) 136, 139, 142, 145, 318, 474, 766
Bsh1236I CGCG 1 cut(s) 376
BshFI GGCC 5 cut(s) 90, 97, 272, 321, 859
BsiHKAI GWGCWC 1 cut(s) 422
BsiSI CCGG 2 cut(s) 187, 361
BslFI GGGAC 2 cut(s) 175, 595
BslI CCNNNNNNNGG 3 cut(s) 378, 379, 854
BsmAI GTCTC 1 cut(s) 727
BsmFI GGGAC 2 cut(s) 175, 595
BsmI GAATGC 2 cut(s) 29, 640
BsnI GGCC 5 cut(s) 90, 97, 272, 321, 859
Bsp119I TTCGAA 1 cut(s) 629
Bsp1286I GDGCHC 1 cut(s) 422
Bsp143I GATC 2 cut(s) 797, 897
Bsp19I CCATGG 1 cut(s) 516
BspACI CCGC 2 cut(s) 374, 968
BspANI GGCC 5 cut(s) 90, 97, 272, 321, 859
BspCNI CTCAG 2 cut(s) 434, 577
BspFNI CGCG 1 cut(s) 376
BspHI TCATGA 1 cut(s) 937
BspLI GGNNCC 2 cut(s) 191, 858
BspPI GGATC 1 cut(s) 792
BspT104I TTCGAA 1 cut(s) 629
BspTI CTTAAG 1 cut(s) 326
BsrBI CCGCTC 1 cut(s) 970
BsrDI GCAATG 1 cut(s) 759
BsrI ACTGG 1 cut(s) 659
BssECI CCNNGG 6 cut(s) 40, 85, 91, 394, 516, 802
BssMI GATC 2 cut(s) 797, 897
BssT1I CCWWGG 3 cut(s) 40, 91, 516
Bst2UI CCWGG 2 cut(s) 395, 770
Bst4CI ACNGT 7 cut(s) 33, 465, 539, 686, 790, 886, 905
BstAFI CTTAAG 1 cut(s) 326
BstAPI GCANNNNNTGC 2 cut(s) 417, 779
BstBI TTCGAA 1 cut(s) 629
BstC8I GCNNGC 4 cut(s) 9, 135, 413, 926
BstDEI CTNAG 3 cut(s) 421, 585, 720
BstDSI CCRYGG 3 cut(s) 85, 516, 802
BstF5I GGATG 1 cut(s) 198
BstFNI CGCG 1 cut(s) 376
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 2 cut(s) 800, 900
BstMAI GTCTC 1 cut(s) 727
BstMBI GATC 2 cut(s) 797, 897
BstMWI GCNNNNNNNGC 5 cut(s) 134, 155, 158, 417, 779
BstNI CCWGG 2 cut(s) 395, 770
BstNSI RCATGY 3 cut(s) 15, 415, 711
BstSCI CCNGG 2 cut(s) 393, 768
BstSFI CTRYAG 2 cut(s) 786, 999
BstUI CGCG 1 cut(s) 376
BstV1I GCAGC 7 cut(s) 136, 139, 142, 145, 318, 474, 766
BstX2I RGATCY 1 cut(s) 797
BstYI RGATCY 1 cut(s) 797
BsuI GTATCC 1 cut(s) 472
BsuRI GGCC 5 cut(s) 90, 97, 272, 321, 859
BtgI CCRYGG 3 cut(s) 85, 516, 802
BtgZI GCGATG 1 cut(s) 391
BtsCI GGATG 1 cut(s) 198
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 2 cut(s) 422, 795
Cac8I GCNNGC 4 cut(s) 9, 135, 413, 926
CaiI CAGNNNCTG 1 cut(s) 779
CciI TCATGA 1 cut(s) 937
CfoI GCGC 1 cut(s) 230
Cfr13I GGNCC 3 cut(s) 95, 189, 857
Csp6I GTAC 3 cut(s) 461, 906, 955
CviAII CATG 6 cut(s) 12, 412, 483, 517, 708, 938
CviQI GTAC 3 cut(s) 461, 906, 955
DdeI CTNAG 3 cut(s) 421, 585, 720
DpnI GATC 2 cut(s) 799, 899
DpnII GATC 2 cut(s) 797, 897
Eco130I CCWWGG 3 cut(s) 40, 91, 516
Eco147I AGGCCT 2 cut(s) 272, 321
Eco47I GGWCC 1 cut(s) 189
EcoRI GAATTC 1 cut(s) 426
EcoRII CCWGG 2 cut(s) 393, 768
EcoT14I CCWWGG 3 cut(s) 40, 91, 516
ErhI CCWWGG 3 cut(s) 40, 91, 516
FaeI CATG 6 cut(s) 15, 415, 486, 520, 711, 941
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 2 cut(s) 175, 595
FatI CATG 6 cut(s) 11, 411, 482, 516, 707, 937
FauI CCCGC 1 cut(s) 381
FblI GTMKAC 1 cut(s) 467
Fnu4HI GCNGC 7 cut(s) 150, 153, 156, 159, 332, 488, 780
FokI GGATG 1 cut(s) 185
Fsp4HI GCNGC 7 cut(s) 150, 153, 156, 159, 332, 488, 780
FspBI CTAG 3 cut(s) 215, 251, 384
GlaI GCGC 1 cut(s) 229
GluI GCNGC 7 cut(s) 150, 153, 156, 159, 332, 488, 780
GsuI CTGGAG 1 cut(s) 831
HaeIII GGCC 5 cut(s) 90, 97, 272, 321, 859
HapII CCGG 2 cut(s) 187, 361
HhaI GCGC 1 cut(s) 230
Hin1II CATG 6 cut(s) 15, 415, 486, 520, 711, 941
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 3 cut(s) 436, 512, 823
HpaII CCGG 2 cut(s) 187, 361
HphI GGTGA 1 cut(s) 834
Hpy166II GTNNAC 4 cut(s) 391, 468, 834, 1030
Hpy188I TCNGA 1 cut(s) 435
Hpy188III TCNNGA 4 cut(s) 307, 423, 743, 938
Hpy8I GTNNAC 4 cut(s) 391, 468, 834, 1030
HpyAV CCTTC 8 cut(s) 68, 113, 117, 214, 311, 332, 1042, 1063
HpyCH4III ACNGT 7 cut(s) 33, 465, 539, 686, 790, 886, 905
HpyCH4IV ACGT 1 cut(s) 839
HpyCH4V TGCA 6 cut(s) 411, 415, 448, 638, 944, 1040
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 155, 158, 417, 779
HpyF3I CTNAG 3 cut(s) 421, 585, 720
HpySE526I ACGT 1 cut(s) 839
Hsp92II CATG 6 cut(s) 15, 415, 486, 520, 711, 941
HspAI GCGC 1 cut(s) 228
Kzo9I GATC 2 cut(s) 797, 897
LmnI GCTCC 3 cut(s) 363, 655, 787
Lsp1109I GCAGC 7 cut(s) 136, 139, 142, 145, 318, 474, 766
LweI GCATC 2 cut(s) 681, 931
MaeI CTAG 3 cut(s) 215, 251, 384
MaeII ACGT 1 cut(s) 839
MaeIII GTNAC 4 cut(s) 840, 886, 1003, 1020
MalI GATC 2 cut(s) 799, 899
MbiI CCGCTC 1 cut(s) 970
MboI GATC 2 cut(s) 797, 897
MboII GAAGA 2 cut(s) 643, 1086
MflI RGATCY 1 cut(s) 797
MhlI GDGCHC 1 cut(s) 422
MluCI AATT 6 cut(s) 45, 426, 554, 565, 589, 1061
MlyI GAGTC 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 321
MseI TTAA 2 cut(s) 123, 327
MspCI CTTAAG 1 cut(s) 326
MspI CCGG 2 cut(s) 187, 361
MspR9I CCNGG 2 cut(s) 395, 770
Mva1269I GAATGC 2 cut(s) 29, 640
MvaI CCWGG 2 cut(s) 395, 770
MvnI CGCG 1 cut(s) 376
MwoI GCNNNNNNNGC 5 cut(s) 134, 155, 158, 417, 779
NcoI CCATGG 1 cut(s) 516
NdeII GATC 2 cut(s) 797, 897
NlaIII CATG 6 cut(s) 15, 415, 486, 520, 711, 941
NlaIV GGNNCC 2 cut(s) 191, 858
NmuCI GTSAC 2 cut(s) 840, 886
NspI RCATGY 3 cut(s) 15, 415, 711
NspV TTCGAA 1 cut(s) 629
PaeI GCATGC 1 cut(s) 415
PagI TCATGA 1 cut(s) 937
PceI AGGCCT 2 cut(s) 272, 321
PciI ACATGT 1 cut(s) 707
PctI GAATGC 2 cut(s) 29, 640
PdmI GAANNNNTTC 1 cut(s) 321
PfeI GAWTC 2 cut(s) 512, 823
PkrI GCNGC 7 cut(s) 151, 154, 157, 160, 333, 489, 781
PleI GAGTC 1 cut(s) 444
PpsI GAGTC 1 cut(s) 444
PscI ACATGT 1 cut(s) 707
Psp6I CCWGG 2 cut(s) 393, 768
PspGI CCWGG 2 cut(s) 393, 768
PspN4I GGNNCC 2 cut(s) 191, 858
PspPI GGNCC 3 cut(s) 95, 189, 857
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 1 cut(s) 797
RsaI GTAC 3 cut(s) 462, 907, 956
RsaNI GTAC 3 cut(s) 461, 906, 955
SaqAI TTAA 2 cut(s) 123, 327
SatI GCNGC 7 cut(s) 150, 153, 156, 159, 332, 488, 780
Sau3AI GATC 2 cut(s) 797, 897
Sau96I GGNCC 3 cut(s) 95, 189, 857
SchI GAGTC 1 cut(s) 445
ScrFI CCNGG 2 cut(s) 395, 770
SduI GDGCHC 1 cut(s) 422
SfaNI GCATC 2 cut(s) 681, 931
SfcI CTRYAG 2 cut(s) 786, 999
SfuI TTCGAA 1 cut(s) 629
SinI GGWCC 1 cut(s) 189
SmlI CTYRAG 4 cut(s) 115, 305, 326, 743
SmoI CTYRAG 4 cut(s) 115, 305, 326, 743
SphI GCATGC 1 cut(s) 415
Sse9I AATT 6 cut(s) 45, 426, 554, 565, 589, 1061
SseBI AGGCCT 2 cut(s) 272, 321
SsiI CCGC 2 cut(s) 374, 968
SspI AATATT 1 cut(s) 402
SspMI CTAG 3 cut(s) 215, 251, 384
StuI AGGCCT 2 cut(s) 272, 321
StyD4I CCNGG 2 cut(s) 393, 768
StyI CCWWGG 3 cut(s) 40, 91, 516
TaaI ACNGT 7 cut(s) 33, 465, 539, 686, 790, 886, 905
TaiI ACGT 1 cut(s) 842
TaqI TCGA 1 cut(s) 629
TasI AATT 6 cut(s) 45, 426, 554, 565, 589, 1061
TfiI GAWTC 2 cut(s) 512, 823
Tru1I TTAA 2 cut(s) 123, 327
Tru9I TTAA 2 cut(s) 123, 327
TscAI CASTG 2 cut(s) 422, 795
TseFI GTSAC 2 cut(s) 840, 886
TseI GCWGC 7 cut(s) 149, 152, 155, 158, 331, 487, 779
Tsp45I GTSAC 2 cut(s) 840, 886
TspDTI ATGAA 7 cut(s) 50, 275, 419, 540, 801, 926, 1082
TspGWI ACGGA 3 cut(s) 74, 130, 265
TspRI CASTG 2 cut(s) 422, 795
Vha464I CTTAAG 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 189
XapI RAATTY 3 cut(s) 426, 565, 589
XceI RCATGY 3 cut(s) 15, 415, 711
XmiI GTMKAC 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 3 cut(s) 215, 251, 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.