pycom06g21460

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0016850
Physical Location & Seq
Reverse (-)
24091085 .. 24091870
786 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGGAAAACAAGGTCGTAGCTGAATCCCAACGCACTTACGAAGACTTTGAACCCTTCTGCAAATGGCATGGAAGCGACGAGATTGAGCTCCATCTTCGCGGTTTTAAAAAGGAACACCTGAAGGTTCAAATTCATAAGGGAATGGTTACAATCCATGGAGAACGTCCTTTGAAGGAAAGTAATGGAAACACATGGAGCCGCTTCCACAAAGAAATCAAACTTTCAGACAAGTGCGATCCAAATGACGTTCACGCTAAGCTTCACAGTGCAGGAGTCCTTACAGTGACAATGCCTGTAGTAGTCAATGCTGGTGATTATGATGCTAATAAACCAACAAGCTGCGAATTTAAGGGCCAAAGGATGTTAGTTTCTAAACGATTAAAAGTGGGAAAAGATATTACAGTGAAGTTTCTGGCGGCAGTTGCTGTGATTGTGGCTTTCGGATTTGGCGCATATGTTGTTAAATATTATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

159

Amino Acids

17.88

Weight (kDa)

9.07

Isoelectric Point (pI)

27.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 27 - 103 9.8e-14 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016850)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18340
malus_domestica MD06G1239100.v1.1
prunus_persica Prupe.5G242900_v2.0.a1
pyrus_communis pycom06g21460
rosa_chinensis RchiOBHm_Chr7g0177251
rosa_laevigata RLG00000005551
rosa_multiflora Rmu_sc0005310.1_g000013
rosa_roxburghii Rroxscaffold_3G00276010
rosa_rugosa Rorug06G0404200
rosa_samantha Rh7AG004200 Rh7BG004000 Rh7CG003900 Rh7DG003900
rosa_wichuraiana Rw7G000340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 99
AciI CCGC 3 cut(s) 99, 199, 416
AclWI GGATC 1 cut(s) 230
AcsI RAATTY 2 cut(s) 129, 344
AcuI CTGAAG 1 cut(s) 140
AgsI TTSAA 3 cut(s) 50, 128, 172
AluBI AGCT 4 cut(s) 20, 88, 259, 339
AluI AGCT 4 cut(s) 20, 88, 259, 339
Alw21I GWGCWC 1 cut(s) 90
AlwI GGATC 1 cut(s) 230
AlwNI CAGNNNCTG 1 cut(s) 425
AoxI GGCC 1 cut(s) 352
ApeKI GCWGC 1 cut(s) 339
ApoI RAATTY 2 cut(s) 129, 344
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 1 cut(s) 352
AsuHPI GGTGA 1 cut(s) 323
BanII GRGCYC 1 cut(s) 90
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 326
BccI CCATC 1 cut(s) 99
BfmI CTRYAG 1 cut(s) 294
BisI GCNGC 3 cut(s) 199, 340, 417
BlpI GCTNAGC 1 cut(s) 255
BlsI GCNGC 3 cut(s) 200, 341, 418
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 1 cut(s) 197
BmsI GCATC 1 cut(s) 310
BpiI GAAGAC 1 cut(s) 48
Bpu1102I GCTNAGC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 154
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 1 cut(s) 366
BseXI GCAGC 1 cut(s) 326
BsgI GTGCAG 1 cut(s) 288
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 354
BsiHKAI GWGCWC 1 cut(s) 90
BsnI GGCC 1 cut(s) 354
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 1 cut(s) 235
Bsp1720I GCTNAGC 1 cut(s) 255
Bsp19I CCATGG 1 cut(s) 154
BspACI CCGC 3 cut(s) 99, 199, 416
BspANI GGCC 1 cut(s) 354
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 197
BspPI GGATC 1 cut(s) 230
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 1 cut(s) 235
BssT1I CCWWGG 1 cut(s) 154
Bst4CI ACNGT 3 cut(s) 266, 283, 403
BstDEI CTNAG 1 cut(s) 255
BstDSI CCRYGG 1 cut(s) 154
BstF5I GGATG 1 cut(s) 366
BstFNI CGCG 1 cut(s) 99
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 1 cut(s) 238
BstMBI GATC 1 cut(s) 235
BstMWI GCNNNNNNNGC 1 cut(s) 422
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 1 cut(s) 326
BstV2I GAAGAC 1 cut(s) 48
BsuRI GGCC 1 cut(s) 354
BtgI CCRYGG 1 cut(s) 154
BtsCI GGATG 1 cut(s) 366
BtsIMutI CAGTG 3 cut(s) 271, 288, 408
CaiI CAGNNNCTG 1 cut(s) 425
CfoI GCGC 1 cut(s) 452
Cfr13I GGNCC 1 cut(s) 352
CviAII CATG 3 cut(s) 68, 155, 192
CviJI RGCY 7 cut(s) 20, 88, 198, 259, 339, 354, 437
CviKI_1 RGCY 7 cut(s) 20, 88, 198, 259, 339, 354, 437
DdeI CTNAG 1 cut(s) 255
DpnI GATC 1 cut(s) 237
DpnII GATC 1 cut(s) 235
DraI TTTAAA 1 cut(s) 106
Ecl136II GAGCTC 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 90
Eco53kI GAGCTC 1 cut(s) 88
Eco57I CTGAAG 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 3 cut(s) 71, 158, 195
FaiI YATR 8 cut(s) 69, 135, 156, 193, 318, 454, 456, 471
FatI CATG 3 cut(s) 67, 154, 191
FauNDI CATATG 1 cut(s) 454
Fnu4HI GCNGC 3 cut(s) 199, 340, 417
FokI GGATG 1 cut(s) 373
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 3 cut(s) 199, 340, 417
GlaI GCGC 1 cut(s) 451
GluI GCNGC 3 cut(s) 199, 340, 417
HaeIII GGCC 1 cut(s) 354
HhaI GCGC 1 cut(s) 452
Hin1II CATG 3 cut(s) 71, 158, 195
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 2 cut(s) 23, 273
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 226, 443
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 3 cut(s) 64, 115, 166
HpyCH4III ACNGT 3 cut(s) 266, 283, 403
HpyCH4IV ACGT 2 cut(s) 163, 246
HpyCH4V TGCA 2 cut(s) 60, 269
HpyF10VI GCNNNNNNNGC 1 cut(s) 422
HpyF3I CTNAG 1 cut(s) 255
HpySE526I ACGT 2 cut(s) 163, 246
Hsp92II CATG 3 cut(s) 71, 158, 195
HspAI GCGC 1 cut(s) 450
Kzo9I GATC 1 cut(s) 235
LmnI GCTCC 2 cut(s) 93, 195
LpnPI CCDG 5 cut(s) 131, 255, 294, 306, 398
Lsp1109I GCAGC 1 cut(s) 326
LweI GCATC 1 cut(s) 310
MaeII ACGT 2 cut(s) 163, 246
MaeIII GTNAC 2 cut(s) 145, 283
MalI GATC 1 cut(s) 237
MboI GATC 1 cut(s) 235
MboII GAAGA 2 cut(s) 53, 86
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 2 cut(s) 129, 344
MlyI GAGTC 1 cut(s) 282
MseI TTAA 5 cut(s) 105, 348, 380, 462, 475
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 1 cut(s) 422
NcoI CCATGG 1 cut(s) 154
NdeI CATATG 1 cut(s) 454
NdeII GATC 1 cut(s) 235
NlaIII CATG 3 cut(s) 71, 158, 195
NlaIV GGNNCC 1 cut(s) 197
NmuCI GTSAC 1 cut(s) 283
PfeI GAWTC 1 cut(s) 23
PkrI GCNGC 3 cut(s) 200, 341, 418
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
Psp124BI GAGCTC 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 197
PspPI GGNCC 1 cut(s) 352
PstNI CAGNNNCTG 1 cut(s) 425
SacI GAGCTC 1 cut(s) 90
SaqAI TTAA 5 cut(s) 105, 348, 380, 462, 475
SatI GCNGC 3 cut(s) 199, 340, 417
Sau3AI GATC 1 cut(s) 235
Sau96I GGNCC 1 cut(s) 352
SchI GAGTC 1 cut(s) 282
SduI GDGCHC 1 cut(s) 90
SetI ASST 9 cut(s) 15, 22, 90, 120, 126, 166, 249, 261, 341
SfaNI GCATC 1 cut(s) 310
SfcI CTRYAG 1 cut(s) 294
Sse9I AATT 2 cut(s) 129, 344
SsiI CCGC 3 cut(s) 99, 199, 416
SspI AATATT 1 cut(s) 467
SstI GAGCTC 1 cut(s) 90
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 3 cut(s) 266, 283, 403
TaiI ACGT 2 cut(s) 166, 249
TasI AATT 2 cut(s) 129, 344
TauI GCSGC 2 cut(s) 201, 419
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 5 cut(s) 105, 348, 380, 462, 475
Tru9I TTAA 5 cut(s) 105, 348, 380, 462, 475
TscAI CASTG 3 cut(s) 271, 288, 408
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 1 cut(s) 339
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 122
TspRI CASTG 3 cut(s) 271, 288, 408
XapI RAATTY 2 cut(s) 129, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.