pycom07g00510

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006948
Physical Location & Seq
Reverse (-)
480391 .. 482526
2136 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1302 bp
ATGAGCAGTCTTTTATACAAATACAATCCTAATTTTAAGGCCTCAGAGGGAGAGCTGAGGAAGAACCACACTGCGGAGTTCATGGATTCGAATATTTTCCAGCAACAGCATCGTCAGCAGCAACAGCAGAGCTCCAGCTTAACGCGGTATCGGTCAGCTCCAAGCTCATTTCTGATGGAGCATATGGATAACAATGGCGGCGGCGGAGGAGTTGAGGATTTGCGGTATCTTCAACCGTCGAGCCCTGAAGTCGAAACGGTGCTAGCCAGATTCATTTCTTCATGTAATGAACCAGATCACCATGACAATGGCGTGCAGCATCATCAATTTGAAATCGAAGAGAGGCCGGTGAATGTGAAGGAAGAGGCAGGGGACTCTGTTTCTAATCACAATAATGGTTACCCGAATTCTACTCATATGATGTACCAAGCTCCTCAAGCTCAACAAGTTCATGGTTTAGATAGCAGCTCCTTTGCTGCAGTCAACTCTACCGTAATGGAGAATTCAATGATGCAATCGAAAATTGGGGTTGGAAATCGGTCTAATCTTGTTAGACAAAGTAGCTCTCCGGCTGGATTCTTCCCTAACTTGACCGTTGACAATGGCTTCAATGTGATGAAAGACTCGGCGGGATATCGCGCAGGCAATGGTGTAAGTGGAGAAGCCAGTCCATCAACTTCTAGGTTGGGTAATCAACTGAATTTCTCATCTAGGCCATCTTCATACTCACATCGTATGCCTCGGATTGCTGAAAATGAGAATGGGAACTCGGGAGAAGGTAGCCAACCGCAACCGAATCATGGTTTAGGAGATGCTAATGGTAGCAATTCACATTACCACTCTAGCTTTCCAAATGATTCATGGGAGAATTCTTCATTTAATGACCTCAAAAGAGCCAGGGACAATGACGGGAACAAGTTTTCTACTTCGATTGCATTCAAATCACAGAACAATGATTTGGGAAACCACAATCACGGTTTGACTCAACATTTGAGCTTGCCTAAACAATTTGAGATGCCTGCTATGGAGAAGTATTTGCAATTTGAAGATTCTATTCCTTGTAAAATTCGTGCCAAAAGAGGCTTCGCCACTCACCCGCGAAGCATCGCAGAGAGGATGAGAAGGATGCGGATTAGTGAAAGAATGAAGAAATTGCAGGATCTTTTCCCGGACATGGACAAGCAAATAAACACGGCAGAAATGTTGGACTTGGCGGTTGAGTTCATAAAAGACCTTCAGAAACAGGTTAAGACCCTCAAAGATACGAAGGCGAAGTGCAGTTGTTCGAGCAAACAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

434

Amino Acids

48.48

Weight (kDa)

7.32

Isoelectric Point (pI)

54.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 368 - 413 1.3e-07 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 145, 639, 1099
AclWI GGATC 1 cut(s) 1167
AcsI RAATTY 5 cut(s) 406, 502, 700, 868, 1065
AcuI CTGAAG 2 cut(s) 267, 1220
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 3 cut(s) 73, 800, 1174
AgsI TTSAA 6 cut(s) 233, 332, 507, 610, 940, 1046
AjnI CCWGG 1 cut(s) 896
AjuI GAANNNNNNNTTGG 4 cut(s) 941, 973, 1067, 1099
Alw21I GWGCWC 1 cut(s) 134
AlwI GGATC 1 cut(s) 1167
Ama87I CYCGRG 1 cut(s) 769
AoxI GGCC 3 cut(s) 39, 344, 713
ApeKI GCWGC 4 cut(s) 118, 316, 465, 476
ApoI RAATTY 5 cut(s) 406, 502, 700, 868, 1065
ArsI GACNNNNNNTTYG 2 cut(s) 973, 1005
Asp700I GAANNNNTTC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 641
AsuC2I CCSGG 1 cut(s) 1169
AsuHPI GGTGA 3 cut(s) 290, 361, 1085
AsuII TTCGAA 1 cut(s) 89
AsuNHI GCTAGC 1 cut(s) 262
AvaI CYCGRG 1 cut(s) 769
BanII GRGCYC 2 cut(s) 134, 245
Bbv12I GWGCWC 1 cut(s) 134
BbvCI CCTCAGC 1 cut(s) 56
BbvI GCAGC 4 cut(s) 130, 328, 463, 477
BccI CCATC 3 cut(s) 169, 679, 724
BceAI ACGGC 1 cut(s) 1209
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 1 cut(s) 898
BcnI CCSGG 1 cut(s) 1169
BfaI CTAG 4 cut(s) 263, 681, 711, 843
BfmI CTRYAG 1 cut(s) 477
BisI GCNGC 6 cut(s) 119, 199, 202, 317, 466, 477
BlsI GCNGC 6 cut(s) 120, 200, 203, 318, 467, 478
Bme1390I CCNGG 2 cut(s) 898, 1169
BmeT110I CYCGRG 1 cut(s) 769
BmrFI CCNGG 2 cut(s) 898, 1169
BmsI GCATC 7 cut(s) 118, 328, 501, 802, 1005, 1113, 1116
BmtI GCTAGC 1 cut(s) 266
BpmI CTGGAG 1 cut(s) 118
Bpu10I CCTNAGC 1 cut(s) 56
Bpu14I TTCGAA 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 420
BpuMI CCSGG 1 cut(s) 1169
BsaJI CCNNGG 2 cut(s) 740, 897
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 800, 1174
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 1 cut(s) 666
Bse3DI GCAATG 1 cut(s) 652
BseBI CCWGG 1 cut(s) 898
BseDI CCNNGG 2 cut(s) 740, 897
BseGI GGATG 2 cut(s) 1122, 1131
BseLI CCNNNNNNNGG 3 cut(s) 73, 800, 1174
BseMI GCAATG 1 cut(s) 652
BseMII CTCAG 2 cut(s) 47, 57
BseNI ACTGG 1 cut(s) 666
BseRI GAGGAG 2 cut(s) 222, 423
BseXI GCAGC 4 cut(s) 130, 328, 463, 477
BsgI GTGCAG 2 cut(s) 335, 1297
Bsh1236I CGCG 3 cut(s) 145, 639, 1099
BshFI GGCC 3 cut(s) 41, 346, 715
BsiHKAI GWGCWC 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 769
BsiSI CCGG 3 cut(s) 347, 569, 1169
BslFI GGGAC 2 cut(s) 386, 914
BslI CCNNNNNNNGG 3 cut(s) 73, 800, 1174
BsmFI GGGAC 2 cut(s) 386, 914
BsmI GAATGC 1 cut(s) 935
BsnI GGCC 3 cut(s) 41, 346, 715
BsoBI CYCGRG 1 cut(s) 769
Bsp119I TTCGAA 1 cut(s) 89
Bsp1286I GDGCHC 2 cut(s) 134, 245
Bsp143I GATC 2 cut(s) 295, 1159
BspANI GGCC 3 cut(s) 41, 346, 715
BspCNI CTCAG 2 cut(s) 48, 56
BspFNI CGCG 3 cut(s) 145, 639, 1099
BspMAI CTGCAG 1 cut(s) 481
BspOI GCTAGC 1 cut(s) 266
BspPI GGATC 1 cut(s) 1167
BspT104I TTCGAA 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 652
BsrFI RCCGGY 1 cut(s) 346
BsrI ACTGG 1 cut(s) 666
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 2 cut(s) 740, 897
BssMI GATC 2 cut(s) 295, 1159
Bst2UI CCWGG 1 cut(s) 898
Bst4CI ACNGT 5 cut(s) 237, 259, 493, 595, 977
Bst6I CTCTTC 2 cut(s) 333, 357
BstBI TTCGAA 1 cut(s) 89
BstC8I GCNNGC 5 cut(s) 264, 314, 643, 998, 1020
BstDEI CTNAG 2 cut(s) 43, 56
BstEII GGTNACC 1 cut(s) 398
BstF5I GGATG 2 cut(s) 1122, 1131
BstFNI CGCG 3 cut(s) 145, 639, 1099
BstHHI GCGC 1 cut(s) 641
BstKTI GATC 2 cut(s) 298, 1162
BstMBI GATC 2 cut(s) 295, 1159
BstMWI GCNNNNNNNGC 3 cut(s) 115, 124, 437
BstNI CCWGG 1 cut(s) 898
BstPI GGTNACC 1 cut(s) 398
BstSCI CCNGG 2 cut(s) 896, 1167
BstSFI CTRYAG 1 cut(s) 477
BstUI CGCG 3 cut(s) 145, 639, 1099
BstV1I GCAGC 4 cut(s) 130, 328, 463, 477
BstX2I RGATCY 1 cut(s) 1159
BstXI CCANNNNNNTGG 1 cut(s) 308
BstYI RGATCY 1 cut(s) 1159
BsuRI GGCC 3 cut(s) 41, 346, 715
BtgZI GCGATG 1 cut(s) 1090
BtsCI GGATG 2 cut(s) 1122, 1131
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 5 cut(s) 264, 314, 643, 998, 1020
CfoI GCGC 1 cut(s) 641
Cfr10I RCCGGY 1 cut(s) 346
Csp6I GTAC 1 cut(s) 424
CviAII CATG 7 cut(s) 82, 282, 302, 452, 800, 861, 1174
CviQI GTAC 1 cut(s) 424
DdeI CTNAG 2 cut(s) 43, 56
DpnI GATC 2 cut(s) 297, 1161
DpnII GATC 2 cut(s) 295, 1159
Eam1104I CTCTTC 2 cut(s) 333, 357
EarI CTCTTC 2 cut(s) 333, 357
EciI GGCGGA 1 cut(s) 219
Ecl136II GAGCTC 1 cut(s) 132
Eco147I AGGCCT 1 cut(s) 41
Eco24I GRGCYC 2 cut(s) 134, 245
Eco32I GATATC 1 cut(s) 635
Eco53kI GAGCTC 1 cut(s) 132
Eco57I CTGAAG 2 cut(s) 267, 1220
Eco88I CYCGRG 1 cut(s) 769
Eco91I GGTNACC 1 cut(s) 398
EcoICRI GAGCTC 1 cut(s) 132
EcoO65I GGTNACC 1 cut(s) 398
EcoRI GAATTC 3 cut(s) 406, 502, 868
EcoRII CCWGG 1 cut(s) 896
EcoRV GATATC 1 cut(s) 635
EcoT38I GRGCYC 2 cut(s) 134, 245
FaeI CATG 7 cut(s) 85, 285, 305, 455, 803, 864, 1177
FaqI GGGAC 2 cut(s) 386, 914
FatI CATG 7 cut(s) 81, 281, 301, 451, 799, 860, 1173
FauI CCCGC 2 cut(s) 622, 1104
FauNDI CATATG 2 cut(s) 183, 417
Fnu4HI GCNGC 6 cut(s) 119, 199, 202, 317, 466, 477
FokI GGATG 2 cut(s) 1129, 1138
FriOI GRGCYC 2 cut(s) 134, 245
Fsp4HI GCNGC 6 cut(s) 119, 199, 202, 317, 466, 477
FspBI CTAG 4 cut(s) 263, 681, 711, 843
GlaI GCGC 1 cut(s) 640
GluI GCNGC 6 cut(s) 119, 199, 202, 317, 466, 477
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 3 cut(s) 41, 346, 715
HapII CCGG 3 cut(s) 347, 569, 1169
HhaI GCGC 1 cut(s) 641
Hin1II CATG 7 cut(s) 85, 285, 305, 455, 803, 864, 1177
Hin6I GCGC 1 cut(s) 639
HinP1I GCGC 1 cut(s) 639
HincII GTYRAC 2 cut(s) 484, 598
HindII GTYRAC 2 cut(s) 484, 598
HinfI GANTC 9 cut(s) 86, 270, 374, 576, 623, 796, 857, 982, 1049
HpaII CCGG 3 cut(s) 347, 569, 1169
HphI GGTGA 3 cut(s) 290, 361, 1085
Hpy166II GTNNAC 2 cut(s) 484, 598
Hpy188I TCNGA 4 cut(s) 46, 174, 744, 1239
Hpy188III TCNNGA 1 cut(s) 771
Hpy8I GTNNAC 2 cut(s) 484, 598
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 5 cut(s) 352, 770, 1116, 1244, 1261
HpyCH4III ACNGT 5 cut(s) 237, 259, 493, 595, 977
HpyCH4V TGCA 7 cut(s) 316, 479, 514, 935, 1039, 1156, 1278
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 124, 437
HpyF3I CTNAG 2 cut(s) 43, 56
Hsp92II CATG 7 cut(s) 85, 285, 305, 455, 803, 864, 1177
HspAI GCGC 1 cut(s) 639
Kzo9I GATC 2 cut(s) 295, 1159
LmnI GCTCC 5 cut(s) 137, 163, 178, 436, 473
Lsp1109I GCAGC 4 cut(s) 130, 328, 463, 477
LweI GCATC 7 cut(s) 118, 328, 501, 802, 1005, 1113, 1116
MaeI CTAG 4 cut(s) 263, 681, 711, 843
MaeIII GTNAC 1 cut(s) 398
MalI GATC 2 cut(s) 297, 1161
MboI GATC 2 cut(s) 295, 1159
MflI RGATCY 1 cut(s) 1159
MhlI GDGCHC 2 cut(s) 134, 245
MlyI GAGTC 3 cut(s) 368, 617, 976
MmeI TCCRAC 2 cut(s) 511, 1185
MroXI GAANNNNTTC 1 cut(s) 95
MseI TTAA 4 cut(s) 36, 140, 879, 1248
MslI CAYNNNNRTG 2 cut(s) 306, 393
MspI CCGG 3 cut(s) 347, 569, 1169
MspR9I CCNGG 2 cut(s) 898, 1169
Mva1269I GAATGC 1 cut(s) 935
MvaI CCWGG 1 cut(s) 898
MvnI CGCG 3 cut(s) 145, 639, 1099
MwoI GCNNNNNNNGC 3 cut(s) 115, 124, 437
NciI CCSGG 1 cut(s) 1169
NdeI CATATG 2 cut(s) 183, 417
NdeII GATC 2 cut(s) 295, 1159
NheI GCTAGC 1 cut(s) 262
NlaIII CATG 7 cut(s) 85, 285, 305, 455, 803, 864, 1177
NmeAIII GCCGAG 1 cut(s) 605
NspV TTCGAA 1 cut(s) 89
PceI AGGCCT 1 cut(s) 41
PctI GAATGC 1 cut(s) 935
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 6 cut(s) 86, 270, 576, 796, 857, 1049
PfoI TCCNGGA 1 cut(s) 1167
PkrI GCNGC 6 cut(s) 120, 200, 203, 318, 467, 478
PleI GAGTC 3 cut(s) 368, 617, 976
PpsI GAGTC 3 cut(s) 368, 617, 976
Psp124BI GAGCTC 1 cut(s) 134
Psp6I CCWGG 1 cut(s) 896
PspEI GGTNACC 1 cut(s) 398
PspGI CCWGG 1 cut(s) 896
PstI CTGCAG 1 cut(s) 481
PsuI RGATCY 1 cut(s) 1159
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
RseI CAYNNNNRTG 2 cut(s) 306, 393
SacI GAGCTC 1 cut(s) 134
SaqAI TTAA 4 cut(s) 36, 140, 879, 1248
SatI GCNGC 6 cut(s) 119, 199, 202, 317, 466, 477
Sau3AI GATC 2 cut(s) 295, 1159
SchI GAGTC 3 cut(s) 368, 617, 976
ScrFI CCNGG 2 cut(s) 898, 1169
SduI GDGCHC 2 cut(s) 134, 245
SfaNI GCATC 7 cut(s) 118, 328, 501, 802, 1005, 1113, 1116
SfcI CTRYAG 1 cut(s) 477
SfuI TTCGAA 1 cut(s) 89
SmiMI CAYNNNNRTG 2 cut(s) 306, 393
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
SseBI AGGCCT 1 cut(s) 41
SspI AATATT 1 cut(s) 94
SspMI CTAG 4 cut(s) 263, 681, 711, 843
SstI GAGCTC 1 cut(s) 134
StuI AGGCCT 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 896, 1167
TaaI ACNGT 5 cut(s) 237, 259, 493, 595, 977
TaqI TCGA 7 cut(s) 89, 239, 252, 336, 518, 929, 1286
TaqII GACCGA 2 cut(s) 141, 528
TauI GCSGC 2 cut(s) 201, 204
TfiI GAWTC 6 cut(s) 86, 270, 576, 796, 857, 1049
Tru1I TTAA 4 cut(s) 36, 140, 879, 1248
Tru9I TTAA 4 cut(s) 36, 140, 879, 1248
TscAI CASTG 1 cut(s) 76
TseI GCWGC 4 cut(s) 118, 316, 465, 476
TspRI CASTG 1 cut(s) 76
XapI RAATTY 5 cut(s) 406, 502, 700, 868, 1065
XcmI CCANNNNNNNNNTGG 1 cut(s) 858
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 4 cut(s) 263, 681, 711, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.