pycom07g00900

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006949
Physical Location & Seq
Forward (+)
766784 .. 767212
429 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g00900.1

Sequence Viewer

Length: 429 bp
ATGCCTGTCGACGCTGACGAAATATATGATGCCGCTGGAGTCTGCATTGGTGTCATCGCCATAATCATACTCATATACTGGATCTCTTACTGCAAGCGGAGACAATCTCCGGTTGACCCGTCGGACCATGACGAGAGCTCCGCAACCATAGAACAAGGGCTTGATGAAGCTACAATATGCAGCTACCCGAAACTACCCTATTCCGAGGCCAAGCTTCAAATGGTTGGTTCAGTTTCATCACCTTCTTGCTGTTCTATATGCTTGGCAGATTTTGATGACACAGATACATTGAGAAGTGTGCCTAACTGTGGCCATTTTTTTCACCAAATTTGTGTGGATAAATGGTTGCAGCTCCACCCCACTTGCCCGATCTGCAGAACGTCGCCTCTGGATCCATCTGTGGGATCGATGAATACTTCTGAGGTGTAA

Protein Analysis

143

Amino Acids

15.55

Weight (kDa)

4.42

Isoelectric Point (pI)

61.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 82 - 126 4.4e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 83 - 126 1.3e-13 Ring finger domain
zf-RING_11 PF17123 84 - 112 2.1e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 84 - 125 1.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 84 - 123 9.2e-07 RING-type zinc-finger
zf-C3HC4 PF00097 84 - 125 3.3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 9
AciI CCGC 3 cut(s) 33, 97, 141
AclWI GGATC 4 cut(s) 89, 386, 399, 412
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 1 cut(s) 327
AfiI CCNNNNNNNGG 2 cut(s) 308, 401
AgsI TTSAA 1 cut(s) 218
AluBI AGCT 5 cut(s) 138, 170, 183, 214, 352
AluI AGCT 5 cut(s) 138, 170, 183, 214, 352
Alw21I GWGCWC 1 cut(s) 140
Alw26I GTCTC 1 cut(s) 94
AlwI GGATC 4 cut(s) 89, 386, 399, 412
AoxI GGCC 2 cut(s) 207, 310
ApeKI GCWGC 2 cut(s) 180, 349
ApoI RAATTY 1 cut(s) 327
AspS9I GGNCC 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 231, 314
AvaII GGWCC 1 cut(s) 124
BalI TGGCCA 1 cut(s) 312
BamHI GGATCC 1 cut(s) 391
BanII GRGCYC 1 cut(s) 140
Bbv12I GWGCWC 1 cut(s) 140
BbvI GCAGC 2 cut(s) 192, 361
BccI CCATC 1 cut(s) 403
BcoDI GTCTC 1 cut(s) 94
BfmI CTRYAG 1 cut(s) 373
BisI GCNGC 3 cut(s) 33, 181, 350
BlsI GCNGC 3 cut(s) 34, 182, 351
Bme18I GGWCC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 124
BmiI GGNNCC 1 cut(s) 393
BmsI GCATC 1 cut(s) 19
BplI GAGNNNNNCTC 2 cut(s) 91, 123
BpmI CTGGAG 1 cut(s) 57
Bsa29I ATCGAT 1 cut(s) 407
BsaJI CCNNGG 1 cut(s) 204
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 2 cut(s) 308, 401
Bse1I ACTGG 1 cut(s) 83
BseCI ATCGAT 1 cut(s) 407
BseDI CCNNGG 1 cut(s) 204
BseLI CCNNNNNNNGG 2 cut(s) 308, 401
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 83
BseXI GCAGC 2 cut(s) 192, 361
BshFI GGCC 2 cut(s) 209, 312
BshVI ATCGAT 1 cut(s) 407
BsiHKAI GWGCWC 1 cut(s) 140
BsiSI CCGG 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 308, 401
BsmAI GTCTC 1 cut(s) 94
BsnI GGCC 2 cut(s) 209, 312
Bsp1286I GDGCHC 1 cut(s) 140
Bsp143I GATC 4 cut(s) 81, 369, 391, 404
BspACI CCGC 3 cut(s) 33, 97, 141
BspANI GGCC 2 cut(s) 209, 312
BspCNI CTCAG 1 cut(s) 412
BspDI ATCGAT 1 cut(s) 407
BspLI GGNNCC 1 cut(s) 393
BspMAI CTGCAG 1 cut(s) 377
BspPI GGATC 4 cut(s) 89, 386, 399, 412
BsrI ACTGG 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 204
BssMI GATC 4 cut(s) 81, 369, 391, 404
Bst4CI ACNGT 1 cut(s) 308
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 420
BstKTI GATC 4 cut(s) 84, 372, 394, 407
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 4 cut(s) 81, 369, 391, 404
BstMWI GCNNNNNNNGC 1 cut(s) 372
BstSFI CTRYAG 1 cut(s) 373
BstV1I GCAGC 2 cut(s) 192, 361
BstX2I RGATCY 2 cut(s) 81, 391
BstYI RGATCY 2 cut(s) 81, 391
Bsu15I ATCGAT 1 cut(s) 407
BsuRI GGCC 2 cut(s) 209, 312
BsuTUI ATCGAT 1 cut(s) 407
BtgZI GCGATG 1 cut(s) 40
Cac8I GCNNGC 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 124
ClaI ATCGAT 1 cut(s) 407
CseI GACGC 1 cut(s) 20
CspCI CAANNNNNGTGG 2 cut(s) 344, 379
CviAII CATG 1 cut(s) 128
CviJI RGCY 8 cut(s) 138, 160, 170, 183, 209, 214, 312, 352
CviKI_1 RGCY 8 cut(s) 138, 160, 170, 183, 209, 214, 312, 352
DdeI CTNAG 1 cut(s) 420
DpnI GATC 4 cut(s) 83, 371, 393, 406
DpnII GATC 4 cut(s) 81, 369, 391, 404
EaeI YGGCCR 1 cut(s) 310
Ecl136II GAGCTC 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 140
Eco47I GGWCC 1 cut(s) 124
Eco53kI GAGCTC 1 cut(s) 138
EcoICRI GAGCTC 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 140
FaeI CATG 1 cut(s) 131
FatI CATG 1 cut(s) 127
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 3 cut(s) 33, 181, 350
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 3 cut(s) 33, 181, 350
GluI GCNGC 3 cut(s) 33, 181, 350
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 2 cut(s) 209, 312
HapII CCGG 1 cut(s) 110
HgaI GACGC 1 cut(s) 20
Hin1II CATG 1 cut(s) 131
HincII GTYRAC 2 cut(s) 10, 115
HindII GTYRAC 2 cut(s) 10, 115
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 1 cut(s) 39
HpaII CCGG 1 cut(s) 110
HphI GGTGA 2 cut(s) 231, 314
Hpy166II GTNNAC 2 cut(s) 10, 115
Hpy188I TCNGA 3 cut(s) 124, 205, 421
Hpy188III TCNNGA 1 cut(s) 389
Hpy8I GTNNAC 2 cut(s) 10, 115
Hpy99I CGWCG 3 cut(s) 14, 124, 385
HpyAV CCTTC 1 cut(s) 252
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 5 cut(s) 45, 93, 180, 349, 375
HpyF10VI GCNNNNNNNGC 1 cut(s) 372
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 1 cut(s) 380
Hsp92II CATG 1 cut(s) 131
Kzo9I GATC 4 cut(s) 81, 369, 391, 404
LmnI GCTCC 2 cut(s) 143, 357
LpnPI CCDG 5 cut(s) 18, 21, 64, 123, 374
Lsp1109I GCAGC 2 cut(s) 192, 361
LweI GCATC 1 cut(s) 19
MaeII ACGT 1 cut(s) 380
MalI GATC 4 cut(s) 83, 371, 393, 406
MboI GATC 4 cut(s) 81, 369, 391, 404
MflI RGATCY 2 cut(s) 81, 391
MhlI GDGCHC 1 cut(s) 140
MlsI TGGCCA 1 cut(s) 312
MluCI AATT 1 cut(s) 327
MluNI TGGCCA 1 cut(s) 312
MlyI GAGTC 1 cut(s) 48
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 3 cut(s) 199, 396, 415
Mox20I TGGCCA 1 cut(s) 312
MscI TGGCCA 1 cut(s) 312
Msp20I TGGCCA 1 cut(s) 312
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 1 cut(s) 110
MwoI GCNNNNNNNGC 1 cut(s) 372
NdeII GATC 4 cut(s) 81, 369, 391, 404
NlaIII CATG 1 cut(s) 131
NlaIV GGNNCC 1 cut(s) 393
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PkrI GCNGC 3 cut(s) 34, 182, 351
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
Psp124BI GAGCTC 1 cut(s) 140
PspN4I GGNNCC 1 cut(s) 393
PspPI GGNCC 1 cut(s) 124
PstI CTGCAG 1 cut(s) 377
PsuI RGATCY 2 cut(s) 81, 391
SacI GAGCTC 1 cut(s) 140
SalI GTCGAC 1 cut(s) 8
SatI GCNGC 3 cut(s) 33, 181, 350
Sau3AI GATC 4 cut(s) 81, 369, 391, 404
Sau96I GGNCC 1 cut(s) 124
SchI GAGTC 1 cut(s) 48
SduI GDGCHC 1 cut(s) 140
SetI ASST 8 cut(s) 140, 172, 185, 216, 244, 354, 383, 426
SfaNI GCATC 1 cut(s) 19
SfcI CTRYAG 1 cut(s) 373
SinI GGWCC 1 cut(s) 124
Sse9I AATT 1 cut(s) 327
SsiI CCGC 3 cut(s) 33, 97, 141
SstI GAGCTC 1 cut(s) 140
TaaI ACNGT 1 cut(s) 308
TaiI ACGT 1 cut(s) 383
TaqI TCGA 2 cut(s) 9, 407
TasI AATT 1 cut(s) 327
TauI GCSGC 1 cut(s) 35
TseI GCWGC 2 cut(s) 180, 349
TspDTI ATGAA 3 cut(s) 180, 225, 425
VpaK11BI GGWCC 1 cut(s) 124
XapI RAATTY 1 cut(s) 327
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XmiI GTMKAC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.