Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0006949

Physical Location & Seq

Genomic Coordinates Forward (+)

1600759 .. 1604317

Gene Structure 3559 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0007700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3138 bp

ATGGCTGCTGCTTCTTCTTGCTCTGCTATCATCCCTCGGAAGAAATATGATGTATTTCTTAGTTTCAGAGGCGAGGACACCCGGTATGCTTTTACCAGCCATCTTTATGAGGCTTTACTTCGGGCCAAAATCCAAACTTACATAGATAACAAATTGGAGAGAGGAGATGAAATCGCACCTGCCCTTCTCAAAGAAATCAGTGAATCGAAGCTTTCGGTTATCATTTTCTCCAAAAACTATGCTTCTTCTGCATGGTGCTTGGATGAACTTGTGCACATACTCGAATGTAGAGATCTCTATAAACAGTTTGTTATACCCATCTTCTACGGCGTAGATCCATCAGATGTACGCAAACAATGTGGGAGTTATGCAGATGCATTTGTTGAACATGAGAAACGTTTCAAGGACCCAATGAACAAGGTGCTCAGGTGGAGAAATGCTTTGACAACAGCAGCCAATATATCTGGGTTTGATTCCCAAACAATTAGGCCCGAGTCTGAATTAGTTAAGAGGGTGGTCAATGACATTTTGGCGAAATTGGATGGCAAATCGCAAAACGCAGGTGGTTTGGAGCGCTGGGTTGGAATGGACAAACACATAATTCAACTTGTTGAGTTATTATGCCTCAACTCACGGGATGTCCACATTGTAGGCATTTGGGGTATGGGTGGTATCGGTAAGACCACCATTGCTGATGCTGTATATCACCGTCTCTCTTTTGAATTCGAAGCTTGCTGCTTTATTAAAGATGTTAGGGAAAAAGCATCAGAGACGAAACATGGACTACCATATGATTTGCGAAATGATCTTTTTCGTACTCTACTAAAGGATGAAAAAATACGCGCAGACACCCCCTCTATCGGATCAACTTTTATGAGAGACAGGCTTGGCAGTACAAAGGCTCTCATTGTTCTTGATGATGTGAGTGAGTTTTCCCAATTAGAATTTTTTGCTGGAGGCGATTTCAATCTGTTTGGCCCTGGAAGTAGAATCCTTGTAACAACCAGAAATAAACGCATATTTCGGAACGGTGATGAGATATATGAGGTTGAGGAATTAGACGTCGATGAAGCTCTTGAGCTCTTTTATTCGAGAGCTTTTAGAAATAAGTCTCCCCCGGGTGATTATACAACTTTTGCAAAAGAGGTGGTAGATTATGCTGGAGGCAATCCATTGGCTCTTACAATCTTGGGCTCCGTATTCTGTCATTGCAAGAGCAAAGAAGACTGGGAAGGTGAATTAGATAAATTGAAAAAATTTCCCGACAAAGGAATCCATAAGGTATTGAGGTTAAGTTATGATGGCTTAGGAGAAAATGAGAAGGAGATATTTCTCGATATCGCTTGTTGTCACAAAGGAAAGCATATTGATGAGGCAAAAAGAATTTTAAATGCCTGTGGTTTTTCTGCCAATGCGGGAGTGAGACTTCTCATTGATATGTCTCTCATATCCGTGGATCTTCGTTCAAGACTTGTTGAGATGCATGATTTGATACAAGAAATGGGTCGGGAAATTGTTCGTGATCAATGTGTTAAAAATCCTGAAAAACGCAGTAGATTGTGGCATGCAAATGATGTTTGTCATGTACTGACAAATAACACGGGTACTGAAGACATTGAGTGCATGGCCATGAATATGGATCTCATTAAAGATTTTTATGTGAATGTTGCATCCTTCGAAAAGATGCATAAGCTAAGATTACTAGATCTATGCTCGTACACCAGGGGTGTCAACAAATTGCACCTTCCTCCAGGTTTCGAGTTTCTCCCTGAGGCCCTTATATATCTGAGGTGGCATTTCTATCCCTTGACATCTCTGCCATCGAGGTTTTCTCCAGAAAACCTTGTCGAGCTTCATATGCCCAACAGCCGACTTGAGCAACTTTGGAATAAAGGCGTGCAGATCAATCTTGGGAGCATAAAACGTATCGATCTTAGTCGCTCCGAGCACCTGGCTGATGTTTCAGCTCTGATTGAGAGTCCAAATCTCGAGAGCATTGACCTTTGGGGTTGTAAAAGTTTGGTTCAACTTCCTTCATCGTTTCAGAATCTTGCCAAGCTTACTTCTCTTAGTCTGGATGGTTGCTCCAATCTCAAAATTCTCCCAGAGATGCCAGGCAATCTGGAAGTCTTATGGTTGAATGGGACTGCAATAGAAGAATTGCCTTCATCAATTTGGTCTCTCGAGAAGCTTCATCTTTTGGATCTTCATATATGTAAAGAGCTTAAGAATCTTCCAAGCAGCACTTGTAGGTTGAATTCGCTCCCAAATCTTCATCTAGCTGGCTGCCGCTCTCTTGAGTCTTTGCCTGTCGAGCTACCTTCTGGGCTGAAGAGACTGCAGTTGACAAATTGCAAGAGTCTTGGGTCTTTGCCTGTCGAGCTACCTTCTGGGCTGGAGTACCTGGAGTTGAGCGATTGTGAGAGTCTTGGGTCTTTGCCTGCCCAGCTACCTTCTGGGCTGAAGGAATTGGACTTGAGAAATTGCAAGAGTCTTGGGTCTTTGCCTGTCGAGCTACCTTCTGGGCTGGAGTACCTGGACTTGAGCGATTGTGAGAGTCTTGGGTCTTTGCCTGTCGAGCTACCTTCTGGGCTGAAGACACTGGAGTTGGTCTCTTGCAAGAGTCTTGGGTCTTTGCCTGTCGAGCTACCTTCTGGGCTGGAGTACCTGGACTTGAGCGATTGTGAGAGTCTTGGGTCTTTGCCTGTCGAGCTACCTTCTGGGCTGGAGTACCTGGACTTGAGCGATTGTGAGAGTCTTGGGTCTTTGCCTGTCGAGCTACCTTCTGGGCTGAAGGAACTGAAGTTGAGAAATTGCAAGAGTCTTGGGTCTTTGCCTGTCGAGCTACCTTCTGGGCTGAAGTACCTGGACTTGAGCTATTGCAAGAGTCTTGGGTCTTTGCCTGTCGAGCTACCTTCTGGGCTGAAGGAACTGAAGTTGAGAAATTGCAAGAGTCTTGGGTCTTTACCGAAGCTGCCCTGGCTTCTAGAACTGGTTAATGCACATGGCTGCACGTCACTAGAGACAGTCTCAAGTCCGATGAGGACTGCACCCATACAAGGTAGGGATCAATATATCAACCCATACAGTCTCCATCAACTATTATTTATGAATTGCGTGAAATTGGATCATGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

1045

Amino Acids

116.83

Weight (kDa)

5.62

Isoelectric Point (pI)

52.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	15 - 186	5.5e-57	TIR domain
TIR_2	PF13676	18 - 114	3.9e-13	TIR domain
NB-ARC	PF00931	199 - 370	1.2e-16	NB-ARC domain
WHD_ROQ1	PF23282	438 - 509	3.6e-22	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	709 - 881	2.7e-06	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006949)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g01050
malus_domestica	MD02G1311700.v1.1 MD07G1010800.v1.1
prunus_persica	Prupe.2G013300_v2.0.a1
pyrus_communis	pycom02g26170 pycom07g00900
rosa_chinensis	RchiOBHm_Chr1g0316001 RchiOBHm_Chr1g0316011
rosa_laevigata	RLG00000030709 RLG00000030712 RLG00000030717
rosa_multiflora	Rmu_co8345883.1_g000001 Rmu_sc0002014.1_g000042 Rmu_sc0005954.1_g000006 Rmu_sc0005954.1_g000016
rosa_roxburghii	Rroxscaffold_4G00331200
rosa_rugosa	Rorug01G0007700 Rorug01G0008200
rosa_samantha	Rh1AG018400 Rh1BG011600 Rh1BG012200 Rh1CG015600 Rh1CG015700 Rh1DG011800
rosa_wichuraiana	Rw1G001140 Rw1G001150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	187, 551
AatII	GACGTC	1 cut(s)	1065
Acc36I	ACCTGC	2 cut(s)	187, 551
AccBSI	CCGCTC	1 cut(s)	2292
AccII	CGCG	1 cut(s)	843
AciI	CCGC	2 cut(s)	1415, 2290
AclI	AACGTT	1 cut(s)	397
AclWI	GGATC	7 cut(s)	329, 871, 1464, 1647, 2211, 3075, 3135
AcoI	YGGCCR	1 cut(s)	1626
AcsI	RAATTY	6 cut(s)	722, 944, 1256, 1383, 2097, 2257
AcuI	CTGAAG	9 cut(s)	1629, 2351, 2483, 2615, 2813, 2822, 2879, 2945, 2954
AcyI	GRCGYC	1 cut(s)	1062
AfeI	AGCGCT	1 cut(s)	575
AfiI	CCNNNNNNNGG	3 cut(s)	860, 1268, 3059
AflII	CTTAAG	1 cut(s)	2225
AjiI	CACGTC	1 cut(s)	3015
Alw21I	GWGCWC	4 cut(s)	276, 426, 1083, 1950
Alw44I	GTGCAC	1 cut(s)	272
AlwI	GGATC	7 cut(s)	329, 871, 1464, 1647, 2211, 3075, 3135
Ama87I	CYCGRG	4 cut(s)	491, 1117, 1988, 2183
Aor51HI	AGCGCT	1 cut(s)	575
AoxI	GGCC	5 cut(s)	123, 488, 976, 1626, 1773
ApaLI	GTGCAC	1 cut(s)	272
ApeKI	GCWGC	8 cut(s)	5, 8, 452, 735, 2241, 2286, 2974, 3009
ApoI	RAATTY	6 cut(s)	722, 944, 1256, 1383, 2097, 2257
Asp700I	GAANNNNTTC	2 cut(s)	398, 1515
AspLEI	GCGC	2 cut(s)	576, 845
AspS9I	GGNCC	5 cut(s)	123, 406, 489, 977, 1774
AsuC2I	CCSGG	3 cut(s)	82, 1118, 1119
AsuHPI	GGTGA	4 cut(s)	698, 1043, 1133, 1247
AsuII	TTCGAA	2 cut(s)	726, 1677
AvaI	CYCGRG	4 cut(s)	491, 1117, 1988, 2183
AvaII	GGWCC	1 cut(s)	406
AxyI	CCTNAGG	1 cut(s)	1770
BaeGI	GKGCMC	1 cut(s)	276
BalI	TGGCCA	1 cut(s)	1628
BanII	GRGCYC	2 cut(s)	1083, 1196
BbsI	GAAGAC	3 cut(s)	1230, 1617, 2603
Bbv12I	GWGCWC	4 cut(s)	276, 426, 1083, 1950
BbvI	GCAGC	6 cut(s)	464, 722, 2253, 2273, 2961, 2996
BccI	CCATC	8 cut(s)	108, 326, 346, 536, 1295, 1828, 2072, 3102
BceAI	ACGGC	1 cut(s)	343
BclI	TGATCA	1 cut(s)	1522
BcnI	CCSGG	3 cut(s)	82, 1118, 1119
BfaI	CTAG	4 cut(s)	1703, 2279, 2987, 3020
BfmI	CTRYAG	1 cut(s)	2339
BfoI	RGCGCY	1 cut(s)	577
BfrI	CTTAAG	1 cut(s)	2225
BfuAI	ACCTGC	2 cut(s)	187, 551
BglII	AGATCT	2 cut(s)	292, 1705
BisI	GCNGC	9 cut(s)	6, 9, 453, 736, 2242, 2287, 2290, 2975, 3010
BlsI	GCNGC	9 cut(s)	7, 10, 454, 737, 2243, 2288, 2291, 2976, 3011
Bme18I	GGWCC	1 cut(s)	406
BmeT110I	CYCGRG	4 cut(s)	491, 1117, 1988, 2183
BmgBI	CACGTC	1 cut(s)	3015
BmgT120I	GGNCC	5 cut(s)	123, 406, 489, 977, 1774
BmiI	GGNNCC	2 cut(s)	408, 1195
BmrI	ACTGGG	1 cut(s)	1237
BmsI	GCATC	7 cut(s)	364, 685, 773, 1470, 1674, 1679, 2100
BmuI	ACTGGG	1 cut(s)	1237
BpiI	GAAGAC	3 cut(s)	1230, 1617, 2603
BplI	GAGNNNNNCTC	6 cut(s)	1816, 1848, 2597, 2629, 3014, 3046
Bpu10I	CCTNAGC	2 cut(s)	425, 1306
Bpu14I	TTCGAA	2 cut(s)	726, 1677
BpuEI	CTTGAG	9 cut(s)	1097, 1895, 2318, 2497, 2563, 2695, 2761, 2893, 3016
BpuMI	CCSGG	3 cut(s)	82, 1118, 1119
Bsa29I	ATCGAT	1 cut(s)	1929
BsaBI	GATNNNNATC	1 cut(s)	966
BsaHI	GRCGYC	1 cut(s)	1062
BsaI	GGTCTC	2 cut(s)	2184, 2617
BsaJI	CCNNGG	7 cut(s)	35, 979, 1116, 1117, 1452, 1722, 2978
Bsc4I	CCNNNNNNNGG	3 cut(s)	860, 1268, 3059
Bse1I	ACTGG	3 cut(s)	1232, 2607, 2997
Bse21I	CCTNAGG	1 cut(s)	1770
Bse3DI	GCAATG	2 cut(s)	687, 1207
Bse8I	GATNNNNATC	1 cut(s)	966
BseCI	ATCGAT	1 cut(s)	1929
BseDI	CCNNGG	7 cut(s)	35, 979, 1116, 1117, 1452, 1722, 2978
BseGI	GGATG	7 cut(s)	30, 268, 547, 643, 835, 1670, 2083
BseJI	GATNNNNATC	1 cut(s)	966
BseLI	CCNNNNNNNGG	3 cut(s)	860, 1268, 3059
BseMI	GCAATG	2 cut(s)	687, 1207
BseMII	CTCAG	3 cut(s)	439, 1761, 1778
BseNI	ACTGG	3 cut(s)	1232, 2607, 2997
BseRI	GAGGAG	1 cut(s)	177
BseSI	GKGCMC	1 cut(s)	276
BseXI	GCAGC	6 cut(s)	464, 722, 2253, 2273, 2961, 2996
BseYI	CCCAGC	2 cut(s)	576, 2445
BsgI	GTGCAG	3 cut(s)	1919, 2995, 3033
Bsh1236I	CGCG	1 cut(s)	843
BshFI	GGCC	5 cut(s)	125, 490, 978, 1628, 1775
BshVI	ATCGAT	1 cut(s)	1929
BsiHKAI	GWGCWC	4 cut(s)	276, 426, 1083, 1950
BsiHKCI	CYCGRG	4 cut(s)	491, 1117, 1988, 2183
BsiSI	CCGG	2 cut(s)	82, 1118
BslFI	GGGAC	1 cut(s)	2158
BslI	CCNNNNNNNGG	3 cut(s)	860, 1268, 3059
BsmBI	CGTCTC	2 cut(s)	716, 764
BsmFI	GGGAC	1 cut(s)	2158
BsnI	GGCC	5 cut(s)	125, 490, 978, 1628, 1775
Bso31I	GGTCTC	2 cut(s)	2184, 2617
BsoBI	CYCGRG	4 cut(s)	491, 1117, 1988, 2183
Bsp119I	TTCGAA	2 cut(s)	726, 1677
Bsp1286I	GDGCHC	5 cut(s)	276, 426, 1083, 1196, 1950
BspACI	CCGC	2 cut(s)	1415, 2290
BspANI	GGCC	5 cut(s)	125, 490, 978, 1628, 1775
BspCNI	CTCAG	3 cut(s)	438, 1762, 1779
BspDI	ATCGAT	1 cut(s)	1929
BspFNI	CGCG	1 cut(s)	843
BspLI	GGNNCC	2 cut(s)	408, 1195
BspMAI	CTGCAG	1 cut(s)	2343
BspMI	ACCTGC	2 cut(s)	187, 551
BspPI	GGATC	7 cut(s)	329, 871, 1464, 1647, 2211, 3075, 3135
BspT104I	TTCGAA	2 cut(s)	726, 1677
BspTI	CTTAAG	1 cut(s)	2225
BspTNI	GGTCTC	2 cut(s)	2184, 2617
BsrBI	CCGCTC	1 cut(s)	2292
BsrDI	GCAATG	2 cut(s)	687, 1207
BsrI	ACTGG	3 cut(s)	1232, 2607, 2997
BssECI	CCNNGG	7 cut(s)	35, 979, 1116, 1117, 1452, 1722, 2978
BssNI	GRCGYC	1 cut(s)	1062
Bst4CI	ACNGT	5 cut(s)	306, 710, 1031, 3028, 3089
Bst6I	CTCTTC	1 cut(s)	2327
BstACI	GRCGYC	1 cut(s)	1062
BstAFI	CTTAAG	1 cut(s)	2225
BstBI	TTCGAA	2 cut(s)	726, 1677
BstC8I	GCNNGC	5 cut(s)	733, 1566, 1898, 2284, 2442
BstDEI	CTNAG	8 cut(s)	59, 425, 1306, 1694, 1770, 1787, 1934, 2069
BstDSI	CCRYGG	1 cut(s)	1452
BstF5I	GGATG	7 cut(s)	30, 268, 547, 643, 835, 1670, 2083
BstFNI	CGCG	1 cut(s)	843
BstH2I	RGCGCY	1 cut(s)	577
BstHHI	GCGC	2 cut(s)	576, 845
BstNSI	RCATGY	1 cut(s)	1568
BstSFI	CTRYAG	1 cut(s)	2339
BstSLI	GKGCMC	1 cut(s)	276
BstUI	CGCG	1 cut(s)	843
BstV1I	GCAGC	6 cut(s)	464, 722, 2253, 2273, 2961, 2996
BstV2I	GAAGAC	3 cut(s)	1230, 1617, 2603
BstX2I	RGATCY	6 cut(s)	292, 334, 1456, 1639, 1705, 2203
BstXI	CCANNNNNNTGG	1 cut(s)	1636
BstYI	RGATCY	6 cut(s)	292, 334, 1456, 1639, 1705, 2203
Bsu15I	ATCGAT	1 cut(s)	1929
Bsu36I	CCTNAGG	1 cut(s)	1770
BsuRI	GGCC	5 cut(s)	125, 490, 978, 1628, 1775
BsuTUI	ATCGAT	1 cut(s)	1929
BtgI	CCRYGG	1 cut(s)	1452
BtrI	CACGTC	1 cut(s)	3015
BtsCI	GGATG	7 cut(s)	30, 268, 547, 643, 835, 1670, 2083
BtsIMutI	CAGTG	2 cut(s)	205, 2600
BveI	ACCTGC	2 cut(s)	187, 551
Cac8I	GCNNGC	5 cut(s)	733, 1566, 1898, 2284, 2442
CfoI	GCGC	2 cut(s)	576, 845
Cfr13I	GGNCC	5 cut(s)	123, 406, 489, 977, 1774
Cfr9I	CCCGGG	1 cut(s)	1117
ClaI	ATCGAT	1 cut(s)	1929
CspCI	CAANNNNNGTGG	4 cut(s)	340, 375, 1128, 1163
DdeI	CTNAG	8 cut(s)	59, 425, 1306, 1694, 1770, 1787, 1934, 2069
DraI	TTTAAA	1 cut(s)	1389
EaeI	YGGCCR	1 cut(s)	1626
Eam1104I	CTCTTC	1 cut(s)	2327
EarI	CTCTTC	1 cut(s)	2327
Ecl136II	GAGCTC	1 cut(s)	1081
Eco24I	GRGCYC	2 cut(s)	1083, 1196
Eco31I	GGTCTC	2 cut(s)	2184, 2617
Eco32I	GATATC	1 cut(s)	1339
Eco47I	GGWCC	1 cut(s)	406
Eco47III	AGCGCT	1 cut(s)	575
Eco53kI	GAGCTC	1 cut(s)	1081
Eco57I	CTGAAG	9 cut(s)	1629, 2351, 2483, 2615, 2813, 2822, 2879, 2945, 2954
Eco81I	CCTNAGG	1 cut(s)	1770
Eco88I	CYCGRG	4 cut(s)	491, 1117, 1988, 2183
EcoICRI	GAGCTC	1 cut(s)	1081
EcoO109I	RGGNCCY	2 cut(s)	406, 1774
EcoRI	GAATTC	2 cut(s)	722, 2257
EcoRV	GATATC	1 cut(s)	1339
EcoT22I	ATGCAT	3 cut(s)	379, 1485, 1689
EcoT38I	GRGCYC	2 cut(s)	1083, 1196
Esp3I	CGTCTC	2 cut(s)	716, 764
FalI	AAGNNNNNCTT	2 cut(s)	2230, 2262
FaqI	GGGAC	1 cut(s)	2158
FauI	CCCGC	1 cut(s)	1408
FauNDI	CATATG	2 cut(s)	790, 1857
FbaI	TGATCA	1 cut(s)	1522
Fnu4HI	GCNGC	9 cut(s)	6, 9, 453, 736, 2242, 2287, 2290, 2975, 3010
FokI	GGATG	7 cut(s)	17, 275, 554, 650, 842, 1657, 2090
FriOI	GRGCYC	2 cut(s)	1083, 1196
Fsp4HI	GCNGC	9 cut(s)	6, 9, 453, 736, 2242, 2287, 2290, 2975, 3010
FspBI	CTAG	4 cut(s)	1703, 2279, 2987, 3020
GlaI	GCGC	2 cut(s)	575, 844
GluI	GCNGC	9 cut(s)	6, 9, 453, 736, 2242, 2287, 2290, 2975, 3010
GsaI	CCCAGC	2 cut(s)	580, 2449
HaeII	RGCGCY	1 cut(s)	577
HaeIII	GGCC	5 cut(s)	125, 490, 978, 1628, 1775
HapII	CCGG	2 cut(s)	82, 1118
HhaI	GCGC	2 cut(s)	576, 845
Hin1I	GRCGYC	1 cut(s)	1062
Hin6I	GCGC	2 cut(s)	574, 843
HinP1I	GCGC	2 cut(s)	574, 843
HincII	GTYRAC	2 cut(s)	1732, 2346
HindII	GTYRAC	2 cut(s)	1732, 2346
HindIII	AAGCTT	4 cut(s)	209, 729, 2057, 2189
HpaII	CCGG	2 cut(s)	82, 1118
HphI	GGTGA	4 cut(s)	698, 1043, 1133, 1247
Hpy166II	GTNNAC	5 cut(s)	274, 643, 1719, 1732, 2346
Hpy8I	GTNNAC	5 cut(s)	274, 643, 1719, 1732, 2346
Hpy99I	CGWCG	1 cut(s)	1067
HpyCH4III	ACNGT	5 cut(s)	306, 710, 1031, 3028, 3089
HpyCH4IV	ACGT	4 cut(s)	397, 1062, 1924, 3014
HpyF3I	CTNAG	8 cut(s)	59, 425, 1306, 1694, 1770, 1787, 1934, 2069
HpySE526I	ACGT	4 cut(s)	397, 1062, 1924, 3014
Hsp92I	GRCGYC	1 cut(s)	1062
HspAI	GCGC	2 cut(s)	574, 843
Ksp22I	TGATCA	1 cut(s)	1522
LmnI	GCTCC	6 cut(s)	571, 1199, 1914, 1946, 2090, 2268
Lsp1109I	GCAGC	6 cut(s)	464, 722, 2253, 2273, 2961, 2996
LweI	GCATC	7 cut(s)	364, 685, 773, 1470, 1674, 1679, 2100
MaeI	CTAG	4 cut(s)	1703, 2279, 2987, 3020
MaeII	ACGT	4 cut(s)	397, 1062, 1924, 3014
MaeIII	GTNAC	3 cut(s)	997, 1349, 3015
MbiI	CCGCTC	1 cut(s)	2292
MflI	RGATCY	6 cut(s)	292, 334, 1456, 1639, 1705, 2203
MhlI	GDGCHC	5 cut(s)	276, 426, 1083, 1196, 1950
MlsI	TGGCCA	1 cut(s)	1628
MluNI	TGGCCA	1 cut(s)	1628
MmeI	TCCRAC	1 cut(s)	562
Mox20I	TGGCCA	1 cut(s)	1628
Mph1103I	ATGCAT	3 cut(s)	379, 1485, 1689
MroXI	GAANNNNTTC	2 cut(s)	398, 1515
MscI	TGGCCA	1 cut(s)	1628
MseI	TTAA	9 cut(s)	507, 744, 1292, 1388, 1533, 1647, 2226, 2997, 3136
MslI	CAYNNNNRTG	7 cut(s)	105, 1368, 1436, 1451, 1569, 1628, 1634
Msp20I	TGGCCA	1 cut(s)	1628
MspCI	CTTAAG	1 cut(s)	2225
MspI	CCGG	2 cut(s)	82, 1118
MvnI	CGCG	1 cut(s)	843
NciI	CCSGG	3 cut(s)	82, 1118, 1119
NdeI	CATATG	2 cut(s)	790, 1857
NlaIV	GGNNCC	2 cut(s)	408, 1195
NmuCI	GTSAC	2 cut(s)	1349, 3015
NsiI	ATGCAT	3 cut(s)	379, 1485, 1689
NspI	RCATGY	1 cut(s)	1568
NspV	TTCGAA	2 cut(s)	726, 1677
PaeI	GCATGC	1 cut(s)	1568
PaeR7I	CTCGAG	2 cut(s)	1988, 2183
PaqCI	CACCTGC	2 cut(s)	187, 551
PdmI	GAANNNNTTC	2 cut(s)	398, 1515
PfeI	GAWTC	6 cut(s)	203, 473, 990, 1272, 2047, 2230
PkrI	GCNGC	9 cut(s)	7, 10, 454, 737, 2243, 2288, 2291, 2976, 3011
PpuMI	RGGWCCY	1 cut(s)	406
Psp124BI	GAGCTC	1 cut(s)	1083
Psp1406I	AACGTT	1 cut(s)	397
Psp5II	RGGWCCY	1 cut(s)	406
PspFI	CCCAGC	2 cut(s)	576, 2445
PspN4I	GGNNCC	2 cut(s)	408, 1195
PspPI	GGNCC	5 cut(s)	123, 406, 489, 977, 1774
PspPPI	RGGWCCY	1 cut(s)	406
PstI	CTGCAG	1 cut(s)	2343
PsuI	RGATCY	6 cut(s)	292, 334, 1456, 1639, 1705, 2203
RseI	CAYNNNNRTG	7 cut(s)	105, 1368, 1436, 1451, 1569, 1628, 1634
SacI	GAGCTC	1 cut(s)	1083
SaqAI	TTAA	9 cut(s)	507, 744, 1292, 1388, 1533, 1647, 2226, 2997, 3136
SatI	GCNGC	9 cut(s)	6, 9, 453, 736, 2242, 2287, 2290, 2975, 3010
Sau96I	GGNCC	5 cut(s)	123, 406, 489, 977, 1774
SduI	GDGCHC	5 cut(s)	276, 426, 1083, 1196, 1950
SfaNI	GCATC	7 cut(s)	364, 685, 773, 1470, 1674, 1679, 2100
SfcI	CTRYAG	1 cut(s)	2339
Sfr274I	CTCGAG	2 cut(s)	1988, 2183
SfuI	TTCGAA	2 cut(s)	726, 1677
SinI	GGWCC	1 cut(s)	406
SlaI	CTCGAG	2 cut(s)	1988, 2183
SmaI	CCCGGG	1 cut(s)	1119
SmiMI	CAYNNNNRTG	7 cut(s)	105, 1368, 1436, 1451, 1569, 1628, 1634
SphI	GCATGC	1 cut(s)	1568
SsiI	CCGC	2 cut(s)	1415, 2290
SspMI	CTAG	4 cut(s)	1703, 2279, 2987, 3020
SstI	GAGCTC	1 cut(s)	1083
TaaI	ACNGT	5 cut(s)	306, 710, 1031, 3028, 3089
TaiI	ACGT	4 cut(s)	400, 1065, 1927, 3017
TatI	WGTACW	2 cut(s)	893, 1585
TauI	GCSGC	1 cut(s)	2292
TfiI	GAWTC	6 cut(s)	203, 473, 990, 1272, 2047, 2230
Tru1I	TTAA	9 cut(s)	507, 744, 1292, 1388, 1533, 1647, 2226, 2997, 3136
Tru9I	TTAA	9 cut(s)	507, 744, 1292, 1388, 1533, 1647, 2226, 2997, 3136
TscAI	CASTG	2 cut(s)	205, 2607
TseFI	GTSAC	2 cut(s)	1349, 3015
TseI	GCWGC	8 cut(s)	5, 8, 452, 735, 2241, 2286, 2974, 3009
Tsp45I	GTSAC	2 cut(s)	1349, 3015
TspGWI	ACGGA	2 cut(s)	1186, 1441
TspMI	CCCGGG	1 cut(s)	1117
TspRI	CASTG	2 cut(s)	205, 2607
Vha464I	CTTAAG	1 cut(s)	2225
VneI	GTGCAC	1 cut(s)	272
VpaK11BI	GGWCC	1 cut(s)	406
XapI	RAATTY	6 cut(s)	722, 944, 1256, 1383, 2097, 2257
XbaI	TCTAGA	1 cut(s)	2986
XceI	RCATGY	1 cut(s)	1568
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2453
XhoI	CTCGAG	2 cut(s)	1988, 2183
XmaI	CCCGGG	1 cut(s)	1117
XmnI	GAANNNNTTC	2 cut(s)	398, 1515
XspI	CTAG	4 cut(s)	1703, 2279, 2987, 3020
ZraI	GACGTC	1 cut(s)	1063
Zsp2I	ATGCAT	3 cut(s)	379, 1485, 1689

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.