pycom07g00960

Carotenoid 9,10(9',10')-cleavage dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008044
Physical Location & Seq
Reverse (-)
810533 .. 816524
5992 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1647 bp
ATGGTGGATGTCGAGGTTAGCGACGAGAAGCAGCAGCTCAAGGGCAGCGGCACAGTGATCCCCAACCCAAAACCCAACAAGGGTTATGCTTCAAAAGTTGTGGACTTGATAGAGAAGCTGATTGTGAAGTGGATGTACGACTCTTCTCAGCCTCACCACTACCTCGCCGGCAACTTTGCTCCGGTGGTTGATGAAACTCCTCCCACCACCAACCTCCCTGTCATCGGCCATCTTCCTGAGTGCTTGAATGGAGAGTTTGTCAGGGTGGGGCCCAACCCAAAGTTTGCACCAGTTGCTGGATATCACTGGTTTGATGGAGATGGTATGGTTCATGGTATGCGCATCAAAGATGGCAAAGCAACTTATGTCTCCCGCTACATTAGGACGTCACGTCTTAAGCAAGAAGAATATTTTAGAGGTGCTAAATTTATGAAGATTGGAGACCTTAAGGGGTTTCTCGGCTTACTCATGGTGAACTTACAAATGCTAAGAGCAAAACTGAAAATATTAGATATGTCTTATGGAAATGGGACAGGTAACACGGCTCTCGTATATCACCATGGGAAGCTTCTAGCTCTTTCAGAGGCAGATAAACCTTATGTCCTCAAAGTTTTGGAAGATGGAGATCTGCAAACAGTTGGCTTGCTGGATTATGACAAGAGACTGACACATTCCTTCACTGCTCATCCAAAGGTTGACCCAGTCACTGGTGAGATGTTTACCTTTGGTTACTCACATACACCGCCATATGTCACTTATAGAGTTATTTCGAAGGATGGTTTCATGCATGACCCTATACCGATAACAATAGCAGACCCCGTCATGATGCATGATTTTGCAATTACTGAGAACTATGCAATTTTCATGGATCTCCCCCTGATCTTCAAACCAAAGGAAATGGTGAAGGAAAAAAAGTTTATATTCACATTTGATCAAACAAAAATAGCTCGCTTTGGTTTACTTCCACGGTATGCAAAGAATGAGTCGTTAATCAGATGGTTCGAGCTTCCAAATTGCTTCATTTTCCATAATGCTAATGCTTGGGAGGAGGGGGACGAAGTTGTTTTAATCACATGCCGGCTTGATAATCTTGATTTGGACATGGTCAATGGATCTGTCAAAGAAAAGCTTGAAAACTTCAAAAATGAGCTGTACGAGATGAGATTCAACTTGAAAACTGGTCTAGCATCCCAAAAGAAGCTATCAGAATCGGCTGTAGATTTTCCCAGGGTGAATGAGAGCTACACCGGCAGGAAGCAGCGTTATGTGTACGGAACCTTACTGGACAGCATTGCCAAGGTTACAGGGGTAGCCAAGTTTGATCTGCATGCTGAACCAGAGGTCGGAAAAACACAAATTGAGGTTGGAGGAAATGTCCAAGGCCTCTATGACCTGGGACCTGGCAGATTTGGTTCTGAGGCTATTTTTGTCCCTCGTATTCCTGGCGTTACTTCTGAAGAAGATGATGGCTACTTAATACTCTTTGTTCATGACGAGAACACTGGAAAATCAGCAATTCAAGTAGTTGATGCAAAGACAATGTCATCAGAGCCTGTTGCAGTTGTGGAATTGCCACACAGAGTTCCTTACGGTTTCCATGCCTTCTTCGTGACAGAGGAGCAATTGCAAGAACAAGCAAAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

549

Amino Acids

61.77

Weight (kDa)

6.05

Isoelectric Point (pI)

30.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 59 - 537 2.4e-140 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 389
Acc16I TGCGCA 1 cut(s) 341
AccB7I CCANNNNNTGG 2 cut(s) 296, 707
AciI CCGC 3 cut(s) 48, 373, 743
AclWI GGATC 3 cut(s) 52, 876, 1120
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 1 cut(s) 425
AcuI CTGAAG 1 cut(s) 1476
AcyI GRCGYC 1 cut(s) 386
AfaI GTAC 3 cut(s) 137, 1154, 1271
AfiI CCNNNNNNNGG 5 cut(s) 80, 224, 296, 707, 1343
AflII CTTAAG 2 cut(s) 395, 446
AgsI TTSAA 8 cut(s) 93, 247, 886, 1133, 1141, 1168, 1174, 1520
AhdI GACNNNNNGTC 1 cut(s) 390
AjiI CACGTC 1 cut(s) 392
AjnI CCWGG 4 cut(s) 1226, 1392, 1399, 1441
Alw26I GTCTC 3 cut(s) 373, 435, 655
AlwI GGATC 3 cut(s) 52, 876, 1120
AlwNI CAGNNNCTG 3 cut(s) 296, 707, 1553
AoxI GGCC 3 cut(s) 226, 269, 1381
ApaI GGGCCC 1 cut(s) 273
ApeKI GCWGC 4 cut(s) 31, 34, 45, 1258
ApoI RAATTY 1 cut(s) 425
ArsI GACNNNNNNTTYG 4 cut(s) 968, 1000, 1526, 1558
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 3 cut(s) 269, 270, 1397
AsuHPI GGTGA 6 cut(s) 146, 484, 548, 722, 913, 1243
AsuII TTCGAA 1 cut(s) 770
AvaII GGWCC 1 cut(s) 1397
BaeGI GKGCMC 1 cut(s) 273
BanII GRGCYC 1 cut(s) 273
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
BbvI GCAGC 4 cut(s) 43, 46, 57, 1270
BccI CCATC 8 cut(s) 237, 308, 314, 344, 614, 770, 990, 1460
BceAI ACGGC 1 cut(s) 558
BciT130I CCWGG 4 cut(s) 1228, 1394, 1401, 1443
BclI TGATCA 1 cut(s) 931
BcoDI GTCTC 3 cut(s) 373, 435, 655
BfaI CTAG 2 cut(s) 572, 1184
BfmI CTRYAG 1 cut(s) 1215
BfrI CTTAAG 2 cut(s) 395, 446
BglII AGATCT 1 cut(s) 625
BisI GCNGC 5 cut(s) 32, 35, 46, 49, 1259
BlsI GCNGC 5 cut(s) 33, 36, 47, 50, 1260
Bme1390I CCNGG 4 cut(s) 1228, 1394, 1401, 1443
Bme18I GGWCC 1 cut(s) 1397
BmeRI GACNNNNNGTC 1 cut(s) 390
BmgBI CACGTC 1 cut(s) 392
BmgT120I GGNCC 3 cut(s) 269, 270, 1397
BmiI GGNNCC 4 cut(s) 270, 271, 1276, 1398
BmrFI CCNGG 4 cut(s) 1228, 1394, 1401, 1443
BmrI ACTGGG 1 cut(s) 695
BmsI GCATC 4 cut(s) 351, 816, 1196, 1519
BmuI ACTGGG 1 cut(s) 695
Bpu14I TTCGAA 1 cut(s) 770
BpuEI CTTGAG 1 cut(s) 23
BsaBI GATNNNNATC 1 cut(s) 624
BsaHI GRCGYC 1 cut(s) 386
BsaI GGTCTC 1 cut(s) 435
BsaJI CCNNGG 7 cut(s) 559, 965, 1226, 1227, 1296, 1378, 1393
BsaWI WCCGGW 1 cut(s) 181
BsaXI ACNNNNNCTCC 4 cut(s) 243, 273, 1359, 1389
Bsc4I CCNNNNNNNGG 5 cut(s) 80, 224, 296, 707, 1343
Bse118I RCCGGY 3 cut(s) 167, 1077, 1247
Bse1I ACTGG 7 cut(s) 290, 311, 701, 712, 1183, 1287, 1507
Bse3DI GCAATG 1 cut(s) 1290
Bse8I GATNNNNATC 1 cut(s) 624
BseBI CCWGG 4 cut(s) 1228, 1394, 1401, 1443
BseDI CCNNGG 7 cut(s) 559, 965, 1226, 1227, 1296, 1378, 1393
BseGI GGATG 5 cut(s) 13, 138, 685, 781, 1187
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 5 cut(s) 80, 224, 296, 707, 1343
BseMI GCAATG 1 cut(s) 1290
BseMII CTCAG 4 cut(s) 161, 228, 837, 1407
BseNI ACTGG 7 cut(s) 290, 311, 701, 712, 1183, 1287, 1507
BseRI GAGGAG 3 cut(s) 189, 1061, 1631
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 4 cut(s) 43, 46, 57, 1270
BshFI GGCC 3 cut(s) 228, 271, 1383
BsiSI CCGG 4 cut(s) 168, 182, 1078, 1248
BslFI GGGAC 4 cut(s) 544, 1067, 1410, 1415
BslI CCNNNNNNNGG 5 cut(s) 80, 224, 296, 707, 1343
BsmAI GTCTC 3 cut(s) 373, 435, 655
BsmFI GGGAC 4 cut(s) 544, 1067, 1410, 1415
BsnI GGCC 3 cut(s) 228, 271, 1383
Bso31I GGTCTC 1 cut(s) 435
Bsp119I TTCGAA 1 cut(s) 770
Bsp120I GGGCCC 1 cut(s) 269
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 7 cut(s) 57, 625, 868, 879, 931, 1112, 1321
Bsp19I CCATGG 1 cut(s) 559
BspACI CCGC 3 cut(s) 48, 373, 743
BspANI GGCC 3 cut(s) 228, 271, 1383
BspCNI CTCAG 4 cut(s) 160, 229, 838, 1408
BspHI TCATGA 2 cut(s) 822, 1489
BspLI GGNNCC 4 cut(s) 270, 271, 1276, 1398
BspPI GGATC 3 cut(s) 52, 876, 1120
BspT104I TTCGAA 1 cut(s) 770
BspTI CTTAAG 2 cut(s) 395, 446
BspTNI GGTCTC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 1290
BsrFI RCCGGY 3 cut(s) 167, 1077, 1247
BsrI ACTGG 7 cut(s) 290, 311, 701, 712, 1183, 1287, 1507
BssAI RCCGGY 3 cut(s) 167, 1077, 1247
BssECI CCNNGG 7 cut(s) 559, 965, 1226, 1227, 1296, 1378, 1393
BssMI GATC 7 cut(s) 57, 625, 868, 879, 931, 1112, 1321
BssNI GRCGYC 1 cut(s) 386
BssT1I CCWWGG 3 cut(s) 559, 1296, 1378
Bst2UI CCWGG 4 cut(s) 1228, 1394, 1401, 1443
Bst4CI ACNGT 4 cut(s) 55, 637, 969, 1592
Bst6I CTCTTC 1 cut(s) 148
BstACI GRCGYC 1 cut(s) 386
BstAFI CTTAAG 2 cut(s) 395, 446
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 770
BstC8I GCNNGC 5 cut(s) 169, 644, 949, 1079, 1329
BstDEI CTNAG 5 cut(s) 147, 237, 488, 846, 1416
BstDSI CCRYGG 2 cut(s) 559, 965
BstF5I GGATG 5 cut(s) 13, 138, 685, 781, 1187
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 7 cut(s) 60, 628, 871, 882, 934, 1115, 1324
BstMAI GTCTC 3 cut(s) 373, 435, 655
BstMBI GATC 7 cut(s) 57, 625, 868, 879, 931, 1112, 1321
BstMWI GCNNNNNNNGC 2 cut(s) 293, 1248
BstNI CCWGG 4 cut(s) 1228, 1394, 1401, 1443
BstNSI RCATGY 2 cut(s) 1077, 1331
BstSCI CCNGG 4 cut(s) 1226, 1392, 1399, 1441
BstSFI CTRYAG 1 cut(s) 1215
BstSLI GKGCMC 1 cut(s) 273
BstV1I GCAGC 4 cut(s) 43, 46, 57, 1270
BstX2I RGATCY 3 cut(s) 625, 868, 1112
BstYI RGATCY 3 cut(s) 625, 868, 1112
BsuRI GGCC 3 cut(s) 228, 271, 1383
BtgI CCRYGG 2 cut(s) 559, 965
BtrI CACGTC 1 cut(s) 392
BtsCI GGATG 5 cut(s) 13, 138, 685, 781, 1187
BtsI GCAGTG 1 cut(s) 678
BtsIMutI CAGTG 5 cut(s) 60, 304, 678, 705, 1500
Cac8I GCNNGC 5 cut(s) 169, 644, 949, 1079, 1329
CaiI CAGNNNCTG 3 cut(s) 296, 707, 1553
CciI TCATGA 2 cut(s) 822, 1489
CfoI GCGC 1 cut(s) 342
Cfr10I RCCGGY 3 cut(s) 167, 1077, 1247
Cfr13I GGNCC 3 cut(s) 269, 270, 1397
Csp6I GTAC 3 cut(s) 136, 1153, 1270
CspCI CAANNNNNGTGG 2 cut(s) 81, 116
CviQI GTAC 3 cut(s) 136, 1153, 1270
DdeI CTNAG 5 cut(s) 147, 237, 488, 846, 1416
DpnI GATC 7 cut(s) 59, 627, 870, 881, 933, 1114, 1323
DpnII GATC 7 cut(s) 57, 625, 868, 879, 931, 1112, 1321
DriI GACNNNNNGTC 1 cut(s) 390
EaeI YGGCCR 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 148
Eam1105I GACNNNNNGTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 148
Eco130I CCWWGG 3 cut(s) 559, 1296, 1378
Eco147I AGGCCT 1 cut(s) 1383
Eco24I GRGCYC 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 435
Eco32I GATATC 1 cut(s) 302
Eco47I GGWCC 1 cut(s) 1397
Eco57I CTGAAG 1 cut(s) 1476
EcoO109I RGGNCCY 2 cut(s) 269, 1397
EcoRII CCWGG 4 cut(s) 1226, 1392, 1399, 1441
EcoRV GATATC 1 cut(s) 302
EcoT14I CCWWGG 3 cut(s) 559, 1296, 1378
EcoT22I ATGCAT 2 cut(s) 789, 831
EcoT38I GRGCYC 1 cut(s) 273
ErhI CCWWGG 3 cut(s) 559, 1296, 1378
FalI AAGNNNNNCTT 2 cut(s) 1113, 1145
FaqI GGGAC 4 cut(s) 544, 1067, 1410, 1415
FauI CCCGC 1 cut(s) 380
FauNDI CATATG 1 cut(s) 748
FbaI TGATCA 1 cut(s) 931
Fnu4HI GCNGC 5 cut(s) 32, 35, 46, 49, 1259
FokI GGATG 5 cut(s) 20, 145, 672, 788, 1174
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 5 cut(s) 32, 35, 46, 49, 1259
FspAI RTGCGCAY 1 cut(s) 341
FspBI CTAG 2 cut(s) 572, 1184
FspI TGCGCA 1 cut(s) 341
GlaI GCGC 1 cut(s) 341
GluI GCNGC 5 cut(s) 32, 35, 46, 49, 1259
HaeIII GGCC 3 cut(s) 228, 271, 1383
HapII CCGG 4 cut(s) 168, 182, 1078, 1248
HhaI GCGC 1 cut(s) 342
Hin1I GRCGYC 1 cut(s) 386
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HincII GTYRAC 1 cut(s) 697
HindII GTYRAC 1 cut(s) 697
HindIII AAGCTT 2 cut(s) 566, 1127
HinfI GANTC 4 cut(s) 140, 983, 1164, 1208
HpaII CCGG 4 cut(s) 168, 182, 1078, 1248
HphI GGTGA 6 cut(s) 146, 484, 548, 722, 913, 1243
Hpy166II GTNNAC 6 cut(s) 103, 475, 697, 720, 959, 1270
Hpy188I TCNGA 7 cut(s) 583, 995, 1207, 1346, 1417, 1456, 1549
Hpy188III TCNNGA 5 cut(s) 236, 823, 1091, 1490, 1609
Hpy8I GTNNAC 6 cut(s) 103, 475, 697, 720, 959, 1270
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 4 cut(s) 685, 766, 898, 1612
HpyCH4III ACNGT 4 cut(s) 55, 637, 969, 1592
HpyCH4IV ACGT 2 cut(s) 386, 391
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 1248
HpyF3I CTNAG 5 cut(s) 147, 237, 488, 846, 1416
HpySE526I ACGT 2 cut(s) 386, 391
Hsp92I GRCGYC 1 cut(s) 386
HspAI GCGC 1 cut(s) 340
KroI GCCGGC 2 cut(s) 167, 1077
KroNI GCCGGC 2 cut(s) 169, 1079
Ksp22I TGATCA 1 cut(s) 931
Kzo9I GATC 7 cut(s) 57, 625, 868, 879, 931, 1112, 1321
LmnI GCTCC 2 cut(s) 184, 1618
Lsp1109I GCAGC 4 cut(s) 43, 46, 57, 1270
LweI GCATC 4 cut(s) 351, 816, 1196, 1519
MaeI CTAG 2 cut(s) 572, 1184
MaeII ACGT 2 cut(s) 386, 391
MaeIII GTNAC 8 cut(s) 387, 536, 703, 728, 751, 1300, 1447, 1609
MalI GATC 7 cut(s) 59, 627, 870, 881, 933, 1114, 1323
MboI GATC 7 cut(s) 57, 625, 868, 879, 931, 1112, 1321
MboII GAAGA 9 cut(s) 135, 224, 416, 445, 629, 874, 1469, 1472, 1597
MfeI CAATTG 1 cut(s) 1622
MflI RGATCY 3 cut(s) 625, 868, 1112
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 8 cut(s) 425, 840, 858, 1012, 1356, 1515, 1568, 1622
MlyI GAGTC 2 cut(s) 134, 992
MmeI TCCRAC 2 cut(s) 1324, 1345
Mph1103I ATGCAT 2 cut(s) 789, 831
MroNI GCCGGC 2 cut(s) 167, 1077
MseI TTAA 5 cut(s) 396, 447, 989, 1067, 1475
MspA1I CMGCKG 1 cut(s) 48
MspCI CTTAAG 2 cut(s) 395, 446
MspI CCGG 4 cut(s) 168, 182, 1078, 1248
MspR9I CCNGG 4 cut(s) 1228, 1394, 1401, 1443
MunI CAATTG 1 cut(s) 1622
MvaI CCWGG 4 cut(s) 1228, 1394, 1401, 1443
MwoI GCNNNNNNNGC 2 cut(s) 293, 1248
NaeI GCCGGC 2 cut(s) 169, 1079
NcoI CCATGG 1 cut(s) 559
NdeI CATATG 1 cut(s) 748
NdeII GATC 7 cut(s) 57, 625, 868, 879, 931, 1112, 1321
NgoMIV GCCGGC 2 cut(s) 167, 1077
NlaIV GGNNCC 4 cut(s) 270, 271, 1276, 1398
NmeAIII GCCGAG 1 cut(s) 438
NmuCI GTSAC 4 cut(s) 387, 703, 751, 1609
NsbI TGCGCA 1 cut(s) 341
NsiI ATGCAT 2 cut(s) 789, 831
NspI RCATGY 2 cut(s) 1077, 1331
NspV TTCGAA 1 cut(s) 770
PaeI GCATGC 1 cut(s) 1331
PagI TCATGA 2 cut(s) 822, 1489
PasI CCCWGGG 1 cut(s) 1227
PceI AGGCCT 1 cut(s) 1383
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PdiI GCCGGC 2 cut(s) 169, 1079
PfeI GAWTC 2 cut(s) 1164, 1208
PflFI GACNNNGTC 4 cut(s) 701, 818, 1103, 1540
PflMI CCANNNNNTGG 2 cut(s) 296, 707
PkrI GCNGC 5 cut(s) 33, 36, 47, 50, 1260
PleI GAGTC 2 cut(s) 134, 991
PpsI GAGTC 2 cut(s) 134, 991
PpuMI RGGWCCY 1 cut(s) 1397
Psp5II RGGWCCY 1 cut(s) 1397
Psp6I CCWGG 4 cut(s) 1226, 1392, 1399, 1441
PspGI CCWGG 4 cut(s) 1226, 1392, 1399, 1441
PspN4I GGNNCC 4 cut(s) 270, 271, 1276, 1398
PspOMI GGGCCC 1 cut(s) 269
PspPI GGNCC 3 cut(s) 269, 270, 1397
PspPPI RGGWCCY 1 cut(s) 1397
PstNI CAGNNNCTG 3 cut(s) 296, 707, 1553
PsuI RGATCY 3 cut(s) 625, 868, 1112
PsyI GACNNNGTC 4 cut(s) 701, 818, 1103, 1540
RsaI GTAC 3 cut(s) 137, 1154, 1271
RsaNI GTAC 3 cut(s) 136, 1153, 1270
SaqAI TTAA 5 cut(s) 396, 447, 989, 1067, 1475
SatI GCNGC 5 cut(s) 32, 35, 46, 49, 1259
Sau3AI GATC 7 cut(s) 57, 625, 868, 879, 931, 1112, 1321
Sau96I GGNCC 3 cut(s) 269, 270, 1397
SchI GAGTC 2 cut(s) 134, 992
ScrFI CCNGG 4 cut(s) 1228, 1394, 1401, 1443
SduI GDGCHC 1 cut(s) 273
SfaNI GCATC 4 cut(s) 351, 816, 1196, 1519
SfcI CTRYAG 1 cut(s) 1215
SfuI TTCGAA 1 cut(s) 770
SinI GGWCC 1 cut(s) 1397
SmlI CTYRAG 3 cut(s) 38, 395, 446
SmoI CTYRAG 3 cut(s) 38, 395, 446
SphI GCATGC 1 cut(s) 1331
Sse9I AATT 8 cut(s) 425, 840, 858, 1012, 1356, 1515, 1568, 1622
SseBI AGGCCT 1 cut(s) 1383
SsiI CCGC 3 cut(s) 48, 373, 743
SspI AATATT 2 cut(s) 410, 507
SspMI CTAG 2 cut(s) 572, 1184
StuI AGGCCT 1 cut(s) 1383
StyD4I CCNGG 4 cut(s) 1226, 1392, 1399, 1441
StyI CCWWGG 3 cut(s) 559, 1296, 1378
TaaI ACNGT 4 cut(s) 55, 637, 969, 1592
TaiI ACGT 2 cut(s) 389, 394
TaqI TCGA 3 cut(s) 12, 770, 1002
TasI AATT 8 cut(s) 425, 840, 858, 1012, 1356, 1515, 1568, 1622
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 2 cut(s) 1164, 1208
Tru1I TTAA 5 cut(s) 396, 447, 989, 1067, 1475
Tru9I TTAA 5 cut(s) 396, 447, 989, 1067, 1475
TscAI CASTG 5 cut(s) 60, 311, 685, 712, 1507
TseFI GTSAC 4 cut(s) 387, 703, 751, 1609
TseI GCWGC 4 cut(s) 31, 34, 45, 1258
Tsp45I GTSAC 4 cut(s) 387, 703, 751, 1609
TspDTI ATGAA 7 cut(s) 207, 320, 446, 772, 853, 1009, 1478
TspGWI ACGGA 1 cut(s) 1287
TspRI CASTG 5 cut(s) 60, 311, 685, 712, 1507
Tth111I GACNNNGTC 4 cut(s) 701, 818, 1103, 1540
Van91I CCANNNNNTGG 2 cut(s) 296, 707
Vha464I CTTAAG 2 cut(s) 395, 446
VpaK11BI GGWCC 1 cut(s) 1397
XapI RAATTY 1 cut(s) 425
XceI RCATGY 2 cut(s) 1077, 1331
XspI CTAG 2 cut(s) 572, 1184
ZraI GACGTC 1 cut(s) 387
Zsp2I ATGCAT 2 cut(s) 789, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.