pycom07g01920

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010028
Physical Location & Seq
Forward (+)
1510060 .. 1520137
10078 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3345 bp
ATGGCGTGCGTAAAGGGGGTGAATCGATCGGCCTGCGTGGCGCTTGCTCCGGACGCGCCGTACATGGCGGCAGGGACGATGGCCGGAGCAGTGGATCTGTCGTTCAGCTCCTCCGCCAACATTGAGATCTTCAAGCTTGATTTACAGTCAGAAGATCGTGACCTACCGGTAGTGGGCGAGTCGACGAGCTCTGAGAGATTCAACAGGTTGTCGTGGGCCAAGCCCACTGGATCGGGGTCTCAAGAATTCGGCCTTGGGCTCATCGCTGGTGGACTCGTTGATGGCACTATTGATATCTGGAACCCACAGACTCTGATCCGTCCCGAGGCAGGTGTAAGTGCTTCAGTTGGACATCTCACAAGACATAAAGGCCCTGTTCGTGGTCTTGAATTTAATGCAATTGCACCCAATTTGCTTGCATCTGGGGCTGATGATGGTGAAATTTGTATATGGGACTTGACCAATCCTGCAGAACCATCTCACTTTCCACCTCTCAAGGGTAGTGGTTCTGCTGCCCAGGGTGAGGTTTCATTTTTATCTTGGAATAGCAAGGTCCAACATATATTAGCATCCACTTCATTAAATGGGACAACTGTGATATGGGACCTGAAAAAGCAAAAGCCAGTGATAAGTTTTACAGATTCAGTTAGAAGACGGTGCTCTGTTTTGCAGTGGAATCCTGATATTGCCACTCAGCTAGTTGTTGCTTCAGATGAAGATGGTTCACCGTCTCTGAGGCTGTGGGATATGCGTAATATAATGTCACCAGTTAAAGAGTTTGTCGGGCACACAAAAGGTGTAATTGCAATGTCTTGGTGTCCGAATGACAGCTCTTATCTGCTTACATGTGCCAAGGATAATCGAACTATTTGCTGGGACACAGTTTCTGCAGAGATTGTATGTGAATTGCCGGCAGGCACCAATTGGAATTTTGATGTGCATTGGTATCCAAAAGTACCTGGAGTGATTTCGGCATCTTCATTTGATGGAAAAATTGGAATTTACAATATTGAGGGTTGCAGCAGATATGGAGTTGGGGAAAGTGCTTTTGGTGCAGGACCTCTGAGAGCTCCGAAATGGTATAAGCGCCCAGTTGGAGCATCTTTTGGCTTTGGAGGCAAGATTGTGTCCTTTCAGCATGGTTCTGCTGGTTCAGAGGTTTATATACACAGCTTGGTGACTGAACACAGTTTGGTGAATCGCTCCTCTGAATTTGAAGCTGCTATACAAAATGGGGAAAGGCCTTTACTTCGAGCCTTATGTGAGAAGAAATCGCAGGAGGCTGAATCTGAGGATGACCAGGAAACATGGGGCTTATTAAGGGTTATGTTTGAAGATGATGGGACTGCAAGGACGAAGTTGATCACTCATCTTGGTTTCAGTATGCCCGAGGAAACAAAAGAAGATGTTCCAGAAGATCCATCACAGGAAGTCAATGCACTTGGGCTTGAGGATACAATAACTGATGAAGTGGGTCTTGGGAATGATAAGGAAGCCACCATCTTTCCATCTGATAATGGAGAGGATTTCTTTAATAATCTTCCTAGTCCAAAAGCTGAAACTCCTGTGTCAACCTCTGGTGGAAAATTGTCTGAGGGGGATGCAGTTCCTGTTACAGAAAAAATGGAACAAGAGCCTGATGGACAGGAGGAGAGTGCTGATCGATCGTTAGATGAATCTGTTCAACATGCTTTAGTTGTAGGAGATTATAAGGGTGCTGTTGCAAAATGCATTTCAGTAAATAAAATGGCTGATGCTTTAGTTATTGCTCATGCTGGTGGTGCTTCGTTGTGGGAGAGCACGCTCGATCAGTATCTTAAGATGAGCCATTCGCCTTACCTTAAGATTGTTTCTGCAATGGTGAGCAATGATCTCTTGAGTCTTGTCAACAACAGACCCCTAAAATTCTGGAAAGAAACACTTGCTCTTCTGTGTAGTTTTGCATCCAGAGATGAGTGGACCGTGCTTTGTGATACACTTGCTTCCAAACTTGTAGCTGCTGGTAATACTCTGGCGGCAACTATTTGTTATATATGTGCGGGGAATATTGATAAAACCGTAGAGATTTGGTCAAGAAGTCTGACAACTGAGCATGAGGGGAGATCCTATGTTGACCTTCTCCAGGAATTAATGGAAAAGACAATTGTCCTTGCCTTGGCATCTGGGCAGAAGCGGTTTAGTGCATCCTTGTGCAAACTTGTTGAGAAATATGCTGAAATTTTAGCTAGTCAAGGCCTTCTAACAACAGCATTGGAGTACCTAAAACTCTTAGGTTCTGATGAGTTGTCACCTGAGCTTGTTATCTTAAGAGATCGTATTTCTCTTTCAACCGAACCTGAGAAAGTTACCAAGAATGAAACATTTGGAAACCAACCTGCAACAAGTGGGCCTGTATACACGGCTGACCAATCTAGTTTTGTCGGGTCCTCTTCTCCATATTATCAGGAAACAGTGCCATCACATTTACAATCAGGTGTTCCTGTAAGTCCTTATGGTGAGAGCTACCAAGAACCTGTGAATCCTTCGTATGGAAGAGGAGGATATGTCCCTCCTGCCTCGTATCAGCCTGCATCCCAGCCCCATATGTTTCTTCCTAACCAACCACCTCAGGTTCCACAGGAGAAATTTTCTGCACCTCCTGTTTCCTCTCAGCCTGCAGTAAGGCCTTTCATTCCTTCAACTCCTCCTGTGCTGAAAAATGTGGATCAATATCAGCAGCCAACATTGGGTTCTCAGTTGTATCCTACTTTCCAACCTATGCAACCAGGGCCTGGTTCAACTGCTCCTCTTCAATCACATGTCCCTCCAGTTCCTGTAAATCAACCACATGTTGTAGCTGCTTCTGTTCCTCCAAGAGGATTTATGCCCGTTACTAATCCAGGAGTTGTTCAGGGGCCTCATGTGGGTTCACTGCAGCCACCTAGTCCTACTCACCAAGCTCCAGCCCGAACCCCTGTGGCAGCAGCAGCTCCACCACCCACCATACAGACAGTTGATACATCCAATGTTCCTGCCCATCAAAAATTGGTAATCGCAACATTAACAAGACTTTTCAACGAGACATCAGAGGCGTTGGGAGGTGCACGTGCAAATCCCGGTAAGAAGCGTGAAATAGAAGACAATTCAAGGAAGATAGGTGCGTTGTTTGCCAAACTCAACAGTGGGGACATATCTAGAAATGCTGCTGATAAGCTTGTTCAGCTTTGCCAGGCATTGGATAATGGTGATTTTGGTACTGCCCTGCAAATCCAGGTGCTTCTTACCACTAGTGAATGGGATGAGTGCAACTTTTGGCTTGCAACTCTAAAGAGGATGATCAAGACAAGGCAAAATGTGAGACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
SRA1 Sec16_C WD40

Protein Analysis

1115

Amino Acids

120.19

Weight (kDa)

5.18

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 88 - 156 7.3e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 88 - 237 4.1e-09 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 88 - 232 2.9e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 88 - 251 4.3e-10 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 94 - 263 9e-12 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 137 - 300 9.2e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 172 - 319 8.5e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 197 - 337 3e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 219 - 332 9e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 245 - 338 8.5e-10 WDR5 beta-propeller domain
TPR_GEMI5 PF23774 542 - 694 1.8e-06 GEMI5 TPR domain
TPR_Sec16 PF12931 561 - 756 2.7e-09 Sec16 Sec23-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63460 AT3G63460 AT3G63460
fragaria_vesca FvH4_7g01850 FvH4_7g01850 FvH4_7g01850
malus_domestica MD02G1300000.v1.1 MD07G1024900.v1.1
prunus_persica Prupe.2G024600_v2.0.a1
pyrus_communis pycom02g25200 pycom07g01920
rosa_chinensis RchiOBHm_Chr1g0318481
rosa_laevigata RLG00000030534
rosa_multiflora Rmu_ssc0000021.1_g000001
rosa_roxburghii Rroxscaffold_4G00329170
rosa_rugosa Rorug01G0021600.1
rosa_samantha Rh1AG034000 Rh1BG030000 Rh1CG033500 Rh1DG037100
rosa_wichuraiana Rw1G002700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1710
AarI CACCTGC 1 cut(s) 320
Acc36I ACCTGC 2 cut(s) 320, 2383
AccB1I GGYRCC 1 cut(s) 917
AccB7I CCANNNNNTGG 2 cut(s) 2771, 3214
AccI GTMKAC 2 cut(s) 182, 2394
AccII CGCG 1 cut(s) 56
AccIII TCCGGA 1 cut(s) 49
AciI CCGC 5 cut(s) 68, 114, 2015, 2039, 2173
AclWI GGATC 6 cut(s) 102, 238, 310, 1412, 2097, 2712
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 9 cut(s) 245, 389, 441, 928, 999, 1211, 1904, 2217, 2624
AcuI CTGAAG 2 cut(s) 327, 693
AcvI CACGTG 1 cut(s) 3086
AfaI GTAC 4 cut(s) 62, 957, 2258, 3235
AflII CTTAAG 3 cut(s) 1817, 1841, 2305
AflIII ACRYGT 3 cut(s) 845, 2797, 2827
AgeI ACCGGT 1 cut(s) 166
AhlI ACTAGT 1 cut(s) 3266
AjnI CCWGG 9 cut(s) 516, 958, 1299, 2121, 2764, 2770, 2878, 3207, 3249
AjuI GAANNNNNNNTTGG 8 cut(s) 237, 269, 1032, 1064, 1461, 1493, 2713, 2745
AloI GAACNNNNNNTCC 2 cut(s) 86, 118
Alw21I GWGCWC 5 cut(s) 191, 662, 1072, 1802, 3085
Alw26I GTCTC 4 cut(s) 243, 735, 3053, 3331
Alw44I GTGCAC 1 cut(s) 3081
AlwI GGATC 6 cut(s) 102, 238, 310, 1412, 2097, 2712
AlwNI CAGNNNCTG 5 cut(s) 313, 887, 1610, 2771, 2813
Ama87I CYCGRG 2 cut(s) 323, 1388
Aor13HI TCCGGA 1 cut(s) 49
ApaLI GTGCAC 1 cut(s) 3081
ApoI RAATTY 9 cut(s) 245, 389, 441, 928, 999, 1211, 1904, 2217, 2624
AseI ATTAAT 1 cut(s) 2129
AsiGI ACCGGT 1 cut(s) 166
Asp700I GAANNNNTTC 2 cut(s) 1407, 1680
AspLEI GCGC 3 cut(s) 43, 58, 1089
AsuC2I CCSGG 1 cut(s) 3096
AvaI CYCGRG 2 cut(s) 323, 1388
AvaII GGWCC 5 cut(s) 553, 604, 1058, 1959, 2424
AxyI CCTNAGG 1 cut(s) 2607
BaeGI GKGCMC 2 cut(s) 789, 3085
BanI GGYRCC 1 cut(s) 917
BanII GRGCYC 3 cut(s) 191, 261, 1072
BarI GAAGNNNNNNTAC 2 cut(s) 1209, 1241
BbrPI CACGTG 1 cut(s) 3086
BbsI GAAGAC 2 cut(s) 658, 3123
Bbv12I GWGCWC 5 cut(s) 191, 662, 1072, 1802, 3085
BceAI ACGGC 2 cut(s) 43, 2415
BcgI CGANNNNNNTGC 4 cut(s) 15, 49, 852, 886
BciT130I CCWGG 9 cut(s) 518, 960, 1301, 2123, 2766, 2772, 2880, 3209, 3251
BciVI GTATCC 3 cut(s) 957, 1447, 2751
BclI TGATCA 2 cut(s) 1362, 3315
BcnI CCSGG 1 cut(s) 3096
BcoDI GTCTC 4 cut(s) 243, 735, 3053, 3331
BcuI ACTAGT 1 cut(s) 3266
BfaI CTAG 7 cut(s) 698, 1545, 2226, 2412, 2922, 3174, 3267
BfmI CTRYAG 4 cut(s) 468, 888, 2655, 2912
BfoI RGCGCY 2 cut(s) 44, 1090
BfrI CTTAAG 3 cut(s) 1817, 1841, 2305
BfuAI ACCTGC 2 cut(s) 320, 2383
BfuI GTATCC 3 cut(s) 957, 1447, 2751
BglI GCCNNNNNGGC 1 cut(s) 38
BglII AGATCT 1 cut(s) 126
Bme18I GGWCC 5 cut(s) 553, 604, 1058, 1959, 2424
BmeT110I CYCGRG 2 cut(s) 323, 1388
BmiI GGNNCC 6 cut(s) 302, 605, 919, 2425, 2613, 2895
BmrI ACTGGG 1 cut(s) 1085
BmuI ACTGGG 1 cut(s) 1085
BoxI GACNNNNGTC 1 cut(s) 2144
BpiI GAAGAC 2 cut(s) 658, 3123
BpmI CTGGAG 4 cut(s) 981, 2105, 2790, 2925
Bpu10I CCTNAGC 1 cut(s) 2292
BpuEI CTTGAG 4 cut(s) 225, 479, 1469, 1897
BpuMI CCSGG 1 cut(s) 3096
Bsa29I ATCGAT 2 cut(s) 25, 1663
BsaAI YACGTR 1 cut(s) 3086
BsaBI GATNNNNATC 2 cut(s) 1812, 2709
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 8 cut(s) 253, 324, 516, 517, 852, 1389, 2154, 2765
BsaWI WCCGGW 2 cut(s) 49, 166
BsaXI ACNNNNNCTCC 2 cut(s) 2529, 2559
Bse118I RCCGGY 2 cut(s) 166, 910
Bse1I ACTGG 5 cut(s) 232, 623, 767, 1091, 2807
Bse21I CCTNAGG 1 cut(s) 2607
Bse3DI GCAATG 3 cut(s) 813, 1863, 1873
Bse8I GATNNNNATC 2 cut(s) 1812, 2709
BseAI TCCGGA 1 cut(s) 49
BseBI CCWGG 9 cut(s) 518, 960, 1301, 2123, 2766, 2772, 2880, 3209, 3251
BseCI ATCGAT 2 cut(s) 25, 1663
BseDI CCNNGG 8 cut(s) 253, 324, 516, 517, 852, 1389, 2154, 2765
BseGI GGATG 9 cut(s) 569, 1300, 1606, 1943, 2183, 2570, 2999, 3283, 3318
BseJI GATNNNNATC 2 cut(s) 1812, 2709
BseMI GCAATG 3 cut(s) 813, 1863, 1873
BseNI ACTGG 5 cut(s) 232, 623, 767, 1091, 2807
BseRI GAGGAG 6 cut(s) 100, 1195, 1664, 2550, 2673, 2775
BseSI GKGCMC 2 cut(s) 789, 3085
BseYI CCCAGC 2 cut(s) 873, 2574
BsgI GTGCAG 2 cut(s) 1074, 2616
Bsh1236I CGCG 1 cut(s) 56
Bsh1285I CGRYCG 2 cut(s) 29, 1667
BshNI GGYRCC 1 cut(s) 917
BshTI ACCGGT 1 cut(s) 166
BshVI ATCGAT 2 cut(s) 25, 1663
BsiEI CGRYCG 2 cut(s) 29, 1667
BsiHKAI GWGCWC 5 cut(s) 191, 662, 1072, 1802, 3085
BsiHKCI CYCGRG 2 cut(s) 323, 1388
BsiSI CCGG 5 cut(s) 50, 84, 167, 911, 3096
BsmAI GTCTC 4 cut(s) 243, 735, 3053, 3331
BsmBI CGTCTC 1 cut(s) 735
Bso31I GGTCTC 1 cut(s) 243
BsoBI CYCGRG 2 cut(s) 323, 1388
Bsp1286I GDGCHC 7 cut(s) 191, 261, 662, 789, 1072, 1802, 3085
Bsp13I TCCGGA 1 cut(s) 49
BspACI CCGC 5 cut(s) 68, 114, 2015, 2039, 2173
BspDI ATCGAT 2 cut(s) 25, 1663
BspEI TCCGGA 1 cut(s) 49
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 6 cut(s) 302, 605, 919, 2425, 2613, 2895
BspMAI CTGCAG 4 cut(s) 472, 892, 2659, 2916
BspMI ACCTGC 2 cut(s) 320, 2383
BspPI GGATC 6 cut(s) 102, 238, 310, 1412, 2097, 2712
BspQI GCTCTTC 1 cut(s) 1932
BspT107I GGYRCC 1 cut(s) 917
BspTI CTTAAG 3 cut(s) 1817, 1841, 2305
BspTNI GGTCTC 1 cut(s) 243
BsrDI GCAATG 3 cut(s) 813, 1863, 1873
BsrFI RCCGGY 2 cut(s) 166, 910
BsrI ACTGG 5 cut(s) 232, 623, 767, 1091, 2807
BssAI RCCGGY 2 cut(s) 166, 910
BssECI CCNNGG 8 cut(s) 253, 324, 516, 517, 852, 1389, 2154, 2765
BssNAI GTATAC 1 cut(s) 2395
BssT1I CCWWGG 3 cut(s) 253, 852, 2154
Bst1107I GTATAC 1 cut(s) 2395
Bst2UI CCWGG 9 cut(s) 518, 960, 1301, 2123, 2766, 2772, 2880, 3209, 3251
Bst6I CTCTTC 4 cut(s) 1932, 2434, 2527, 2793
BstAFI CTTAAG 3 cut(s) 1817, 1841, 2305
BstBAI YACGTR 1 cut(s) 3086
BstENI CCTNNNNNAGG 2 cut(s) 2120, 2853
BstF5I GGATG 9 cut(s) 569, 1300, 1606, 1943, 2183, 2570, 2999, 3283, 3318
BstFNI CGCG 1 cut(s) 56
BstH2I RGCGCY 2 cut(s) 44, 1090
BstHHI GCGC 3 cut(s) 43, 58, 1089
BstMAI GTCTC 4 cut(s) 243, 735, 3053, 3331
BstMCI CGRYCG 2 cut(s) 29, 1667
BstNI CCWGG 9 cut(s) 518, 960, 1301, 2123, 2766, 2772, 2880, 3209, 3251
BstNSI RCATGY 4 cut(s) 849, 1691, 2801, 2831
BstPAI GACNNNNGTC 1 cut(s) 2144
BstSFI CTRYAG 4 cut(s) 468, 888, 2655, 2912
BstSLI GKGCMC 2 cut(s) 789, 3085
BstUI CGCG 1 cut(s) 56
BstV2I GAAGAC 2 cut(s) 658, 3123
BstX2I RGATCY 4 cut(s) 94, 126, 1417, 2102
BstYI RGATCY 4 cut(s) 94, 126, 1417, 2102
BstZ17I GTATAC 1 cut(s) 2395
Bsu15I ATCGAT 2 cut(s) 25, 1663
Bsu36I CCTNAGG 1 cut(s) 2607
BsuI GTATCC 3 cut(s) 957, 1447, 2751
BsuTUI ATCGAT 2 cut(s) 25, 1663
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 9 cut(s) 569, 1300, 1606, 1943, 2183, 2570, 2999, 3283, 3318
BtsI GCAGTG 3 cut(s) 96, 677, 2909
BtsIMutI CAGTG 7 cut(s) 96, 225, 630, 677, 2457, 2909, 3166
BveI ACCTGC 2 cut(s) 320, 2383
CaiI CAGNNNCTG 5 cut(s) 313, 887, 1610, 2771, 2813
CfoI GCGC 3 cut(s) 43, 58, 1089
Cfr10I RCCGGY 2 cut(s) 166, 910
ClaI ATCGAT 2 cut(s) 25, 1663
CseI GACGC 1 cut(s) 62
Csp6I GTAC 4 cut(s) 61, 956, 2257, 3234
CspAI ACCGGT 1 cut(s) 166
CspCI CAANNNNNGTGG 2 cut(s) 484, 519
CviQI GTAC 4 cut(s) 61, 956, 2257, 3234
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 4 cut(s) 1932, 2434, 2527, 2793
EarI CTCTTC 4 cut(s) 1932, 2434, 2527, 2793
EciI GGCGGA 1 cut(s) 103
Ecl136II GAGCTC 2 cut(s) 189, 1070
Eco130I CCWWGG 3 cut(s) 253, 852, 2154
Eco147I AGGCCT 3 cut(s) 1243, 2235, 2665
Eco24I GRGCYC 3 cut(s) 191, 261, 1072
Eco31I GGTCTC 1 cut(s) 243
Eco32I GATATC 1 cut(s) 295
Eco47I GGWCC 5 cut(s) 553, 604, 1058, 1959, 2424
Eco53kI GAGCTC 2 cut(s) 189, 1070
Eco57I CTGAAG 2 cut(s) 327, 693
Eco72I CACGTG 1 cut(s) 3086
Eco81I CCTNAGG 1 cut(s) 2607
Eco88I CYCGRG 2 cut(s) 323, 1388
EcoICRI GAGCTC 2 cut(s) 189, 1070
EcoNI CCTNNNNNAGG 2 cut(s) 2120, 2853
EcoO109I RGGNCCY 6 cut(s) 371, 604, 1058, 2424, 2768, 2894
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 9 cut(s) 516, 958, 1299, 2121, 2764, 2770, 2878, 3207, 3249
EcoRV GATATC 1 cut(s) 295
EcoT14I CCWWGG 3 cut(s) 253, 852, 2154
EcoT22I ATGCAT 1 cut(s) 1733
EcoT38I GRGCYC 3 cut(s) 191, 261, 1072
ErhI CCWWGG 3 cut(s) 253, 852, 2154
Esp3I CGTCTC 1 cut(s) 735
FalI AAGNNNNNCTT 4 cut(s) 1461, 1493, 1905, 1937
FauI CCCGC 1 cut(s) 2032
FauNDI CATATG 1 cut(s) 2583
FbaI TGATCA 2 cut(s) 1362, 3315
FblI GTMKAC 2 cut(s) 182, 2394
FokI GGATG 9 cut(s) 556, 1307, 1613, 1930, 2170, 2557, 2986, 3290, 3325
FriOI GRGCYC 3 cut(s) 191, 261, 1072
FspBI CTAG 7 cut(s) 698, 1545, 2226, 2412, 2922, 3174, 3267
GlaI GCGC 3 cut(s) 42, 57, 1088
GsaI CCCAGC 2 cut(s) 877, 2578
GsuI CTGGAG 4 cut(s) 981, 2105, 2790, 2925
HaeII RGCGCY 2 cut(s) 44, 1090
HapII CCGG 5 cut(s) 50, 84, 167, 911, 3096
HgaI GACGC 1 cut(s) 62
HhaI GCGC 3 cut(s) 43, 58, 1089
Hin6I GCGC 3 cut(s) 41, 56, 1087
HinP1I GCGC 3 cut(s) 41, 56, 1087
HincII GTYRAC 4 cut(s) 183, 1572, 1888, 2113
HindII GTYRAC 4 cut(s) 183, 1572, 1888, 2113
HindIII AAGCTT 2 cut(s) 134, 3191
HpaII CCGG 5 cut(s) 50, 84, 167, 911, 3096
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 4 cut(s) 2126, 2246, 2532, 2685
HpyCH4IV ACGT 1 cut(s) 3085
HpySE526I ACGT 1 cut(s) 3085
HspAI GCGC 3 cut(s) 41, 56, 1087
Kpn2I TCCGGA 1 cut(s) 49
KroI GCCGGC 1 cut(s) 910
KroNI GCCGGC 1 cut(s) 912
Ksp22I TGATCA 2 cut(s) 1362, 3315
LguI GCTCTTC 1 cut(s) 1932
LmnI GCTCC 9 cut(s) 52, 86, 113, 1075, 1097, 1208, 2788, 2944, 2974
MaeI CTAG 7 cut(s) 698, 1545, 2226, 2412, 2922, 3174, 3267
MaeII ACGT 1 cut(s) 3085
MaeIII GTNAC 7 cut(s) 158, 762, 1177, 1612, 2286, 2344, 2869
MfeI CAATTG 3 cut(s) 399, 922, 2142
MflI RGATCY 4 cut(s) 94, 126, 1417, 2102
MhlI GDGCHC 7 cut(s) 191, 261, 662, 789, 1072, 1802, 3085
MlyI GAGTC 4 cut(s) 188, 267, 304, 1888
MmeI TCCRAC 4 cut(s) 328, 580, 1075, 2776
Mph1103I ATGCAT 1 cut(s) 1733
MroI TCCGGA 1 cut(s) 49
MroNI GCCGGC 1 cut(s) 910
MroXI GAANNNNTTC 2 cut(s) 1407, 1680
MslI CAYNNNNRTG 2 cut(s) 1776, 2188
MspCI CTTAAG 3 cut(s) 1817, 1841, 2305
MspI CCGG 5 cut(s) 50, 84, 167, 911, 3096
MunI CAATTG 3 cut(s) 399, 922, 2142
MvaI CCWGG 9 cut(s) 518, 960, 1301, 2123, 2766, 2772, 2880, 3209, 3251
MvnI CGCG 1 cut(s) 56
NaeI GCCGGC 1 cut(s) 912
NciI CCSGG 1 cut(s) 3096
NdeI CATATG 1 cut(s) 2583
NgoMIV GCCGGC 1 cut(s) 910
NlaIV GGNNCC 6 cut(s) 302, 605, 919, 2425, 2613, 2895
NmuCI GTSAC 4 cut(s) 158, 762, 1177, 2286
NsiI ATGCAT 1 cut(s) 1733
NspI RCATGY 4 cut(s) 849, 1691, 2801, 2831
PaqCI CACCTGC 1 cut(s) 320
PasI CCCWGGG 1 cut(s) 517
PceI AGGCCT 3 cut(s) 1243, 2235, 2665
PciI ACATGT 3 cut(s) 845, 2797, 2827
PciSI GCTCTTC 1 cut(s) 1932
PdiI GCCGGC 1 cut(s) 912
PdmI GAANNNNTTC 2 cut(s) 1407, 1680
PfeI GAWTC 8 cut(s) 22, 198, 641, 676, 1198, 1286, 1676, 2518
PflMI CCANNNNNTGG 2 cut(s) 2771, 3214
PfoI TCCNGGA 2 cut(s) 2121, 2878
PinAI ACCGGT 1 cut(s) 166
Ple19I CGATCG 2 cut(s) 29, 1667
PleI GAGTC 4 cut(s) 187, 267, 304, 1887
PmaCI CACGTG 1 cut(s) 3086
PmlI CACGTG 1 cut(s) 3086
PpsI GAGTC 4 cut(s) 187, 267, 304, 1887
Ppu21I YACGTR 1 cut(s) 3086
PpuMI RGGWCCY 3 cut(s) 604, 1058, 2424
PscI ACATGT 3 cut(s) 845, 2797, 2827
PshAI GACNNNNGTC 1 cut(s) 2144
PshBI ATTAAT 1 cut(s) 2129
PsiI TTATAA 1 cut(s) 1710
Psp124BI GAGCTC 2 cut(s) 191, 1072
Psp5II RGGWCCY 3 cut(s) 604, 1058, 2424
Psp6I CCWGG 9 cut(s) 516, 958, 1299, 2121, 2764, 2770, 2878, 3207, 3249
PspCI CACGTG 1 cut(s) 3086
PspFI CCCAGC 2 cut(s) 873, 2574
PspGI CCWGG 9 cut(s) 516, 958, 1299, 2121, 2764, 2770, 2878, 3207, 3249
PspN4I GGNNCC 6 cut(s) 302, 605, 919, 2425, 2613, 2895
PspPPI RGGWCCY 3 cut(s) 604, 1058, 2424
PstI CTGCAG 4 cut(s) 472, 892, 2659, 2916
PstNI CAGNNNCTG 5 cut(s) 313, 887, 1610, 2771, 2813
PsuI RGATCY 4 cut(s) 94, 126, 1417, 2102
PvuI CGATCG 2 cut(s) 29, 1667
RsaI GTAC 4 cut(s) 62, 957, 2258, 3235
RsaNI GTAC 4 cut(s) 61, 956, 2257, 3234
RseI CAYNNNNRTG 2 cut(s) 1776, 2188
SacI GAGCTC 2 cut(s) 191, 1072
SalI GTCGAC 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 1932
SchI GAGTC 4 cut(s) 188, 267, 304, 1888
SduI GDGCHC 7 cut(s) 191, 261, 662, 789, 1072, 1802, 3085
SfcI CTRYAG 4 cut(s) 468, 888, 2655, 2912
SinI GGWCC 5 cut(s) 553, 604, 1058, 1959, 2424
SmiMI CAYNNNNRTG 2 cut(s) 1776, 2188
SmlI CTYRAG 7 cut(s) 240, 494, 1448, 1817, 1841, 1876, 2305
SmoI CTYRAG 7 cut(s) 240, 494, 1448, 1817, 1841, 1876, 2305
SpeI ACTAGT 1 cut(s) 3266
SseBI AGGCCT 3 cut(s) 1243, 2235, 2665
SsiI CCGC 5 cut(s) 68, 114, 2015, 2039, 2173
SspI AATATT 2 cut(s) 1009, 2047
SspMI CTAG 7 cut(s) 698, 1545, 2226, 2412, 2922, 3174, 3267
SstI GAGCTC 2 cut(s) 191, 1072
StuI AGGCCT 3 cut(s) 1243, 2235, 2665
StyI CCWWGG 3 cut(s) 253, 852, 2154
TaiI ACGT 1 cut(s) 3088
TaqI TCGA 6 cut(s) 25, 182, 862, 1252, 1663, 1806
TauI GCSGC 2 cut(s) 71, 2018
TfiI GAWTC 8 cut(s) 22, 198, 641, 676, 1198, 1286, 1676, 2518
TscAI CASTG 7 cut(s) 96, 232, 630, 677, 2457, 2916, 3166
TseFI GTSAC 4 cut(s) 158, 762, 1177, 2286
Tsp45I GTSAC 4 cut(s) 158, 762, 1177, 2286
TspDTI ATGAA 8 cut(s) 519, 567, 729, 969, 1482, 1689, 2370, 2659
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 7 cut(s) 96, 232, 630, 677, 2457, 2916, 3166
Van91I CCANNNNNTGG 2 cut(s) 2771, 3214
Vha464I CTTAAG 3 cut(s) 1817, 1841, 2305
VneI GTGCAC 1 cut(s) 3081
VpaK11BI GGWCC 5 cut(s) 553, 604, 1058, 1959, 2424
VspI ATTAAT 1 cut(s) 2129
XagI CCTNNNNNAGG 2 cut(s) 2120, 2853
XapI RAATTY 9 cut(s) 245, 389, 441, 928, 999, 1211, 1904, 2217, 2624
XbaI TCTAGA 1 cut(s) 3173
XceI RCATGY 4 cut(s) 849, 1691, 2801, 2831
XmiI GTMKAC 2 cut(s) 182, 2394
XmnI GAANNNNTTC 2 cut(s) 1407, 1680
XspI CTAG 7 cut(s) 698, 1545, 2226, 2412, 2922, 3174, 3267
Zsp2I ATGCAT 1 cut(s) 1733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.