pycom07g05850

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008468
Physical Location & Seq
Forward (+)
5121252 .. 5125769
4518 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 873 bp
ATGGTGATGCTTTTGGGTCAAAATTATCATTCCTATTATCCTCCACCCAACCCAACCGCTGCTTCTTCTTCTTCCTCTTCCTCTGCCTTGTATACAACTATTTTCCCTACTTTTTGGGGAAAAGAAAAATTAAAAAATATTTGTTCATATATAAAAACAGAGAAGATGGCACTGAGCTCATTCTCTATAAAATTCGGTTCGATTTCGTGTCCCTACGAAGGCCACAGGACCGGTACGTCTACGAGACTCCTCAAGCTCAACCATGTCCTTTCGGTTCGCGTTTCGGCCGATTCGACCCCAAAAGCCCGATTCGTCGCCCGCCGAACCGAGTCCGTCTCGGTTCCGCAGCTCCAGCGTCCTCTAAGTGAGTATATGAGCTTGCCGGCGAGTCAGTACTCGGTTTTGGACGCGGAGAGGATCGAGAGAGTGGATGCGAATACATTCAGGTGCTATGTGTATAGGTTCAAGTTCTTTGCGTTTGAGGTTTGCCCTGTTCTTCTTGTTAAAGTCGAAGAGCAGCCCAATGGCTGTTGCATTAAGCTCTTGTCCTGCAAGCTGGATGGTTCGCCGATTGCAGTTGCGCAGAATGACAAGTTTGACGCTTTTATGGTGAACCAAATATCGTGTGCTAGCAACCAAACCAACTCCTCACGACAAGAACTCAAATCAGATACTGTCATTGAGGTTAGCATTGAGATTCCTTTTGCATTTCGTGCAATCCCAGTGCAAGCTATTGAATCATCTGGGACCCAAGTCCTTGAACAAGTACTAAGAATTATGCTTCCCAGGTTTATGTCACAGCTTGTGAGGGATTATCGAGCCTGGGCTTCTGGTGATAGATCAAGGCAGCCTCTTGGAACTGGCGAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

291

Amino Acids

32.49

Weight (kDa)

9.07

Isoelectric Point (pI)

51.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 120 - 276 3.3e-43 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 235
Acc16I TGCGCA 1 cut(s) 582
AccI GTMKAC 2 cut(s) 92, 239
AccII CGCG 2 cut(s) 279, 410
AciI CCGC 4 cut(s) 57, 319, 344, 410
AclWI GGATC 1 cut(s) 425
AcoI YGGCCR 1 cut(s) 285
AcsI RAATTY 1 cut(s) 191
AfaI GTAC 3 cut(s) 235, 395, 768
AfiI CCNNNNNNNGG 1 cut(s) 218
AgeI ACCGGT 1 cut(s) 230
AgsI TTSAA 3 cut(s) 466, 737, 761
AjnI CCWGG 2 cut(s) 785, 821
AjuI GAANNNNNNNTTGG 2 cut(s) 46, 78
AluBI AGCT 8 cut(s) 177, 256, 349, 378, 541, 556, 731, 802
AluI AGCT 8 cut(s) 177, 256, 349, 378, 541, 556, 731, 802
Alw21I GWGCWC 1 cut(s) 179
Alw26I GTCTC 2 cut(s) 238, 340
AlwI GGATC 1 cut(s) 425
AlwNI CAGNNNCTG 1 cut(s) 674
AoxI GGCC 2 cut(s) 220, 285
ApeKI GCWGC 4 cut(s) 59, 346, 517, 847
ApoI RAATTY 1 cut(s) 191
AsiGI ACCGGT 1 cut(s) 230
Asp700I GAANNNNTTC 1 cut(s) 440
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 2 cut(s) 228, 747
AsuHPI GGTGA 3 cut(s) 16, 622, 845
AsuNHI GCTAGC 1 cut(s) 629
AvaII GGWCC 2 cut(s) 228, 747
BanII GRGCYC 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 179
BbvI GCAGC 4 cut(s) 46, 358, 529, 859
BccI CCATC 2 cut(s) 160, 554
BciT130I CCWGG 2 cut(s) 787, 823
BcoDI GTCTC 2 cut(s) 238, 340
BfaI CTAG 1 cut(s) 630
BisI GCNGC 4 cut(s) 60, 347, 518, 848
BlsI GCNGC 4 cut(s) 61, 348, 519, 849
BmcAI AGTACT 2 cut(s) 395, 768
Bme1390I CCNGG 2 cut(s) 787, 823
Bme18I GGWCC 2 cut(s) 228, 747
BmgT120I GGNCC 2 cut(s) 228, 747
BmiI GGNNCC 3 cut(s) 342, 748, 749
BmrFI CCNGG 2 cut(s) 787, 823
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 1 cut(s) 421
BmtI GCTAGC 1 cut(s) 633
BmuI ACTGGG 1 cut(s) 716
BoxI GACNNNNGTC 1 cut(s) 752
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpmI CTGGAG 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 236
BsaJI CCNNGG 2 cut(s) 785, 822
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse118I RCCGGY 2 cut(s) 230, 382
Bse1I ACTGG 2 cut(s) 722, 865
BseBI CCWGG 2 cut(s) 787, 823
BseDI CCNNGG 2 cut(s) 785, 822
BseGI GGATG 2 cut(s) 436, 565
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 2 cut(s) 722, 865
BseRI GAGGAG 2 cut(s) 239, 637
BseX3I CGGCCG 1 cut(s) 285
BseXI GCAGC 4 cut(s) 46, 358, 529, 859
Bsh1236I CGCG 2 cut(s) 279, 410
Bsh1285I CGRYCG 1 cut(s) 288
BshFI GGCC 2 cut(s) 222, 287
BshTI ACCGGT 1 cut(s) 230
BsiEI CGRYCG 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 179
BsiSI CCGG 2 cut(s) 231, 383
BslFI GGGAC 2 cut(s) 195, 760
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 2 cut(s) 238, 340
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 2 cut(s) 195, 760
BsnI GGCC 2 cut(s) 222, 287
Bsp1286I GDGCHC 1 cut(s) 179
Bsp143I GATC 2 cut(s) 417, 839
BspACI CCGC 4 cut(s) 57, 319, 344, 410
BspANI GGCC 2 cut(s) 222, 287
BspCNI CTCAG 1 cut(s) 165
BspFNI CGCG 2 cut(s) 279, 410
BspLI GGNNCC 3 cut(s) 342, 748, 749
BspOI GCTAGC 1 cut(s) 633
BspPI GGATC 1 cut(s) 425
BspQI GCTCTTC 1 cut(s) 507
BsrFI RCCGGY 2 cut(s) 230, 382
BsrI ACTGG 2 cut(s) 722, 865
BssAI RCCGGY 2 cut(s) 230, 382
BssECI CCNNGG 2 cut(s) 785, 822
BssMI GATC 2 cut(s) 417, 839
BssNAI GTATAC 1 cut(s) 93
Bst1107I GTATAC 1 cut(s) 93
Bst2UI CCWGG 2 cut(s) 787, 823
Bst4CI ACNGT 1 cut(s) 676
Bst6I CTCTTC 2 cut(s) 82, 507
BstAPI GCANNNNNTGC 1 cut(s) 713
BstC8I GCNNGC 6 cut(s) 319, 380, 384, 554, 631, 729
BstDEI CTNAG 3 cut(s) 173, 362, 770
BstF5I GGATG 2 cut(s) 436, 565
BstFNI CGCG 2 cut(s) 279, 410
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 2 cut(s) 420, 842
BstMAI GTCTC 2 cut(s) 238, 340
BstMBI GATC 2 cut(s) 417, 839
BstMCI CGRYCG 1 cut(s) 288
BstMWI GCNNNNNNNGC 2 cut(s) 352, 713
BstNI CCWGG 2 cut(s) 787, 823
BstPAI GACNNNNGTC 1 cut(s) 752
BstSCI CCNGG 2 cut(s) 785, 821
BstUI CGCG 2 cut(s) 279, 410
BstV1I GCAGC 4 cut(s) 46, 358, 529, 859
BstZ17I GTATAC 1 cut(s) 93
BstZI CGGCCG 1 cut(s) 285
BsuRI GGCC 2 cut(s) 222, 287
BtsCI GGATG 2 cut(s) 436, 565
BtsIMutI CAGTG 2 cut(s) 170, 729
Cac8I GCNNGC 6 cut(s) 319, 380, 384, 554, 631, 729
CaiI CAGNNNCTG 1 cut(s) 674
CfoI GCGC 1 cut(s) 583
Cfr10I RCCGGY 2 cut(s) 230, 382
Cfr13I GGNCC 2 cut(s) 228, 747
CseI GACGC 3 cut(s) 344, 416, 608
Csp6I GTAC 3 cut(s) 234, 394, 767
CspAI ACCGGT 1 cut(s) 230
CviAII CATG 1 cut(s) 263
CviQI GTAC 3 cut(s) 234, 394, 767
DdeI CTNAG 3 cut(s) 173, 362, 770
DpnI GATC 2 cut(s) 419, 841
DpnII GATC 2 cut(s) 417, 839
DrdI GACNNNNNNGTC 1 cut(s) 235
DseDI GACNNNNNNGTC 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 285
EagI CGGCCG 1 cut(s) 285
Eam1104I CTCTTC 2 cut(s) 82, 507
EarI CTCTTC 2 cut(s) 82, 507
Ecl136II GAGCTC 1 cut(s) 177
EclXI CGGCCG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 179
Eco47I GGWCC 2 cut(s) 228, 747
Eco52I CGGCCG 1 cut(s) 285
Eco53kI GAGCTC 1 cut(s) 177
EcoICRI GAGCTC 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 747
EcoRII CCWGG 2 cut(s) 785, 821
EcoT38I GRGCYC 1 cut(s) 179
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 1 cut(s) 266
FaqI GGGAC 2 cut(s) 195, 760
FatI CATG 1 cut(s) 262
FauI CCCGC 1 cut(s) 326
FblI GTMKAC 2 cut(s) 92, 239
Fnu4HI GCNGC 4 cut(s) 60, 347, 518, 848
FokI GGATG 2 cut(s) 443, 572
FriOI GRGCYC 1 cut(s) 179
Fsp4HI GCNGC 4 cut(s) 60, 347, 518, 848
FspBI CTAG 1 cut(s) 630
FspI TGCGCA 1 cut(s) 582
GlaI GCGC 1 cut(s) 582
GluI GCNGC 4 cut(s) 60, 347, 518, 848
GsuI CTGGAG 1 cut(s) 335
HaeIII GGCC 2 cut(s) 222, 287
HapII CCGG 2 cut(s) 231, 383
HgaI GACGC 3 cut(s) 344, 416, 608
HhaI GCGC 1 cut(s) 583
Hin1II CATG 1 cut(s) 266
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HinfI GANTC 7 cut(s) 246, 290, 309, 329, 388, 697, 737
HpaII CCGG 2 cut(s) 231, 383
HphI GGTGA 3 cut(s) 16, 622, 845
Hpy166II GTNNAC 3 cut(s) 93, 240, 613
Hpy188I TCNGA 1 cut(s) 670
Hpy188III TCNNGA 2 cut(s) 421, 651
Hpy8I GTNNAC 3 cut(s) 93, 240, 613
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 1 cut(s) 212
HpyCH4III ACNGT 1 cut(s) 676
HpyCH4IV ACGT 1 cut(s) 236
HpyCH4V TGCA 6 cut(s) 534, 552, 575, 707, 716, 727
HpyF10VI GCNNNNNNNGC 2 cut(s) 352, 713
HpyF3I CTNAG 3 cut(s) 173, 362, 770
HpySE526I ACGT 1 cut(s) 236
Hsp92II CATG 1 cut(s) 266
HspAI GCGC 1 cut(s) 581
KflI GGGWCCC 1 cut(s) 747
KroI GCCGGC 1 cut(s) 382
KroNI GCCGGC 1 cut(s) 384
Kzo9I GATC 2 cut(s) 417, 839
LguI GCTCTTC 1 cut(s) 507
LmnI GCTCC 1 cut(s) 354
Lsp1109I GCAGC 4 cut(s) 46, 358, 529, 859
LweI GCATC 1 cut(s) 421
MaeI CTAG 1 cut(s) 630
MaeII ACGT 1 cut(s) 236
MaeIII GTNAC 1 cut(s) 795
MalI GATC 2 cut(s) 419, 841
MboI GATC 2 cut(s) 417, 839
MboII GAAGA 7 cut(s) 57, 60, 63, 69, 175, 488, 524
MhlI GDGCHC 1 cut(s) 179
MluCI AATT 4 cut(s) 22, 128, 191, 774
MlyI GAGTC 3 cut(s) 240, 338, 397
MroNI GCCGGC 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 440
MseI TTAA 3 cut(s) 131, 504, 537
MslI CAYNNNNRTG 1 cut(s) 445
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 2 cut(s) 231, 383
MspR9I CCNGG 2 cut(s) 787, 823
MvaI CCWGG 2 cut(s) 787, 823
MvnI CGCG 2 cut(s) 279, 410
MwoI GCNNNNNNNGC 2 cut(s) 352, 713
NaeI GCCGGC 1 cut(s) 384
NdeII GATC 2 cut(s) 417, 839
NgoMIV GCCGGC 1 cut(s) 382
NheI GCTAGC 1 cut(s) 629
NlaIII CATG 1 cut(s) 266
NlaIV GGNNCC 3 cut(s) 342, 748, 749
NmuCI GTSAC 1 cut(s) 795
NsbI TGCGCA 1 cut(s) 582
PciSI GCTCTTC 1 cut(s) 507
PcsI WCGNNNNNNNCGW 1 cut(s) 290
PdiI GCCGGC 1 cut(s) 384
PdmI GAANNNNTTC 1 cut(s) 440
PfeI GAWTC 4 cut(s) 290, 309, 697, 737
PinAI ACCGGT 1 cut(s) 230
PkrI GCNGC 4 cut(s) 61, 348, 519, 849
PleI GAGTC 3 cut(s) 240, 337, 396
PpsI GAGTC 3 cut(s) 240, 337, 396
PpuMI RGGWCCY 1 cut(s) 747
PshAI GACNNNNGTC 1 cut(s) 752
Psp124BI GAGCTC 1 cut(s) 179
Psp5II RGGWCCY 1 cut(s) 747
Psp6I CCWGG 2 cut(s) 785, 821
PspGI CCWGG 2 cut(s) 785, 821
PspN4I GGNNCC 3 cut(s) 342, 748, 749
PspPI GGNCC 2 cut(s) 228, 747
PspPPI RGGWCCY 1 cut(s) 747
PstNI CAGNNNCTG 1 cut(s) 674
RsaI GTAC 3 cut(s) 235, 395, 768
RsaNI GTAC 3 cut(s) 234, 394, 767
RseI CAYNNNNRTG 1 cut(s) 445
SacI GAGCTC 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 507
SaqAI TTAA 3 cut(s) 131, 504, 537
SatI GCNGC 4 cut(s) 60, 347, 518, 848
Sau3AI GATC 2 cut(s) 417, 839
Sau96I GGNCC 2 cut(s) 228, 747
ScaI AGTACT 2 cut(s) 395, 768
SchI GAGTC 3 cut(s) 240, 338, 397
ScrFI CCNGG 2 cut(s) 787, 823
SduI GDGCHC 1 cut(s) 179
SfaNI GCATC 1 cut(s) 421
SinI GGWCC 2 cut(s) 228, 747
SmiMI CAYNNNNRTG 1 cut(s) 445
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 4 cut(s) 22, 128, 191, 774
SsiI CCGC 4 cut(s) 57, 319, 344, 410
SspI AATATT 1 cut(s) 139
SspMI CTAG 1 cut(s) 630
SstI GAGCTC 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 785, 821
TaaI ACNGT 1 cut(s) 676
TaiI ACGT 1 cut(s) 239
TaqI TCGA 5 cut(s) 200, 293, 420, 510, 817
TasI AATT 4 cut(s) 22, 128, 191, 774
TatI WGTACW 2 cut(s) 393, 766
TfiI GAWTC 4 cut(s) 290, 309, 697, 737
Tru1I TTAA 3 cut(s) 131, 504, 537
Tru9I TTAA 3 cut(s) 131, 504, 537
TscAI CASTG 2 cut(s) 177, 729
TseFI GTSAC 1 cut(s) 795
TseI GCWGC 4 cut(s) 59, 346, 517, 847
Tsp45I GTSAC 1 cut(s) 795
TspDTI ATGAA 1 cut(s) 135
TspGWI ACGGA 1 cut(s) 322
TspRI CASTG 2 cut(s) 177, 729
VpaK11BI GGWCC 2 cut(s) 228, 747
XapI RAATTY 1 cut(s) 191
XmiI GTMKAC 2 cut(s) 92, 239
XmnI GAANNNNTTC 1 cut(s) 440
XspI CTAG 1 cut(s) 630
ZrmI AGTACT 2 cut(s) 395, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.