The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
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| Species | Orthologous Gene IDs |
|---|---|
| pyrus_communis | pycom07g07040 |
| rosa_multiflora | Rmu_sc0001136.1_g000002 Rmu_sc0001437.1_g000003 Rmu_sc0005385.1_g000021 Rmu_sc0010640.1_g000008 |
| rosa_roxburghii | Rroxscaffold_4G00292690 Rroxscaffold_5G00356490 |
| rosa_samantha | Rh5DG227500 Rh6AG009200 Rh6AG111800 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 733 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 329 |
| AciI | CCGC | 5 cut(s) | 59, 212, 517, 533, 619 |
| AclWI | GGATC | 2 cut(s) | 203, 481 |
| AfaI | GTAC | 1 cut(s) | 553 |
| AgeI | ACCGGT | 1 cut(s) | 554 |
| AgsI | TTSAA | 3 cut(s) | 192, 460, 658 |
| AluBI | AGCT | 2 cut(s) | 340, 481 |
| AluI | AGCT | 2 cut(s) | 340, 481 |
| Alw21I | GWGCWC | 1 cut(s) | 516 |
| AlwI | GGATC | 2 cut(s) | 203, 481 |
| AsiGI | ACCGGT | 1 cut(s) | 554 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 464 |
| AspS9I | GGNCC | 1 cut(s) | 322 |
| AsuHPI | GGTGA | 3 cut(s) | 61, 95, 283 |
| AvaII | GGWCC | 1 cut(s) | 322 |
| BaeGI | GKGCMC | 1 cut(s) | 8 |
| BauI | CACGAG | 1 cut(s) | 356 |
| Bbv12I | GWGCWC | 1 cut(s) | 516 |
| BccI | CCATC | 8 cut(s) | 44, 123, 145, 254, 281, 302, 627, 635 |
| BciVI | GTATCC | 1 cut(s) | 564 |
| BclI | TGATCA | 2 cut(s) | 360, 541 |
| BfuI | GTATCC | 1 cut(s) | 564 |
| BglII | AGATCT | 1 cut(s) | 155 |
| BisI | GCNGC | 1 cut(s) | 534 |
| BlsI | GCNGC | 1 cut(s) | 535 |
| Bme18I | GGWCC | 1 cut(s) | 322 |
| BmgT120I | GGNCC | 1 cut(s) | 322 |
| Bsa29I | ATCGAT | 1 cut(s) | 408 |
| BsaJI | CCNNGG | 1 cut(s) | 318 |
| BsaWI | WCCGGW | 2 cut(s) | 441, 554 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 95, 125, 605, 635 |
| Bse118I | RCCGGY | 1 cut(s) | 554 |
| Bse3DI | GCAATG | 1 cut(s) | 574 |
| BseCI | ATCGAT | 1 cut(s) | 408 |
| BseDI | CCNNGG | 1 cut(s) | 318 |
| BseGI | GGATG | 9 cut(s) | 36, 115, 137, 221, 236, 273, 342, 638, 646 |
| BseMI | GCAATG | 1 cut(s) | 574 |
| BseRI | GAGGAG | 6 cut(s) | 80, 90, 245, 248, 338, 620 |
| BseSI | GKGCMC | 1 cut(s) | 8 |
| BseYI | CCCAGC | 1 cut(s) | 241 |
| BshTI | ACCGGT | 1 cut(s) | 554 |
| BshVI | ATCGAT | 1 cut(s) | 408 |
| BsiHKAI | GWGCWC | 1 cut(s) | 516 |
| BsiSI | CCGG | 4 cut(s) | 398, 442, 555, 592 |
| Bsp1286I | GDGCHC | 2 cut(s) | 8, 516 |
| Bsp143I | GATC | 9 cut(s) | 155, 208, 218, 360, 405, 409, 471, 486, 541 |
| BspACI | CCGC | 5 cut(s) | 59, 212, 517, 533, 619 |
| BspDI | ATCGAT | 1 cut(s) | 408 |
| BspPI | GGATC | 2 cut(s) | 203, 481 |
| BsrDI | GCAATG | 1 cut(s) | 574 |
| BsrFI | RCCGGY | 1 cut(s) | 554 |
| BssAI | RCCGGY | 1 cut(s) | 554 |
| BssECI | CCNNGG | 1 cut(s) | 318 |
| BssMI | GATC | 9 cut(s) | 155, 208, 218, 360, 405, 409, 471, 486, 541 |
| BssSI | CACGAG | 1 cut(s) | 356 |
| Bst2BI | CACGAG | 1 cut(s) | 356 |
| Bst4CI | ACNGT | 1 cut(s) | 331 |
| Bst6I | CTCTTC | 2 cut(s) | 238, 317 |
| BstDEI | CTNAG | 1 cut(s) | 381 |
| BstF5I | GGATG | 9 cut(s) | 36, 115, 137, 221, 236, 273, 342, 638, 646 |
| BstKTI | GATC | 9 cut(s) | 158, 211, 221, 363, 408, 412, 474, 489, 544 |
| BstMBI | GATC | 9 cut(s) | 155, 208, 218, 360, 405, 409, 471, 486, 541 |
| BstSLI | GKGCMC | 1 cut(s) | 8 |
| BstX2I | RGATCY | 2 cut(s) | 155, 208 |
| BstYI | RGATCY | 2 cut(s) | 155, 208 |
| Bsu15I | ATCGAT | 1 cut(s) | 408 |
| BsuI | GTATCC | 1 cut(s) | 564 |
| BsuTUI | ATCGAT | 1 cut(s) | 408 |
| BtsCI | GGATG | 9 cut(s) | 36, 115, 137, 221, 236, 273, 342, 638, 646 |
| Cfr10I | RCCGGY | 1 cut(s) | 554 |
| Cfr13I | GGNCC | 1 cut(s) | 322 |
| ClaI | ATCGAT | 1 cut(s) | 408 |
| Csp6I | GTAC | 1 cut(s) | 552 |
| CspAI | ACCGGT | 1 cut(s) | 554 |
| CviAII | CATG | 3 cut(s) | 250, 394, 545 |
| CviJI | RGCY | 4 cut(s) | 245, 340, 481, 674 |
| CviKI_1 | RGCY | 4 cut(s) | 245, 340, 481, 674 |
| CviQI | GTAC | 1 cut(s) | 552 |
| DdeI | CTNAG | 1 cut(s) | 381 |
| DpnI | GATC | 9 cut(s) | 157, 210, 220, 362, 407, 411, 473, 488, 543 |
| DpnII | GATC | 9 cut(s) | 155, 208, 218, 360, 405, 409, 471, 486, 541 |
| DraI | TTTAAA | 1 cut(s) | 723 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 329 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 329 |
| Eam1104I | CTCTTC | 2 cut(s) | 238, 317 |
| EarI | CTCTTC | 2 cut(s) | 238, 317 |
| EciI | GGCGGA | 1 cut(s) | 201 |
| Eco47I | GGWCC | 1 cut(s) | 322 |
| FaeI | CATG | 3 cut(s) | 253, 397, 548 |
| FaiI | YATR | 5 cut(s) | 132, 251, 395, 546, 733 |
| FatI | CATG | 3 cut(s) | 249, 393, 544 |
| FauI | CCCGC | 1 cut(s) | 66 |
| FbaI | TGATCA | 2 cut(s) | 360, 541 |
| Fnu4HI | GCNGC | 1 cut(s) | 534 |
| FokI | GGATG | 9 cut(s) | 23, 102, 124, 208, 223, 260, 329, 645, 653 |
| Fsp4HI | GCNGC | 1 cut(s) | 534 |
| GluI | GCNGC | 1 cut(s) | 534 |
| GsaI | CCCAGC | 1 cut(s) | 245 |
| HapII | CCGG | 4 cut(s) | 398, 442, 555, 592 |
| Hin1II | CATG | 3 cut(s) | 253, 397, 548 |
| HinfI | GANTC | 2 cut(s) | 182, 377 |
| HpaII | CCGG | 4 cut(s) | 398, 442, 555, 592 |
| HphI | GGTGA | 3 cut(s) | 61, 95, 283 |
| Hpy188I | TCNGA | 3 cut(s) | 181, 208, 319 |
| Hpy188III | TCNNGA | 3 cut(s) | 334, 358, 469 |
| HpyAV | CCTTC | 7 cut(s) | 24, 95, 171, 437, 657, 664, 678 |
| HpyCH4III | ACNGT | 1 cut(s) | 331 |
| HpyCH4IV | ACGT | 2 cut(s) | 306, 587 |
| HpyCH4V | TGCA | 1 cut(s) | 22 |
| HpyF3I | CTNAG | 1 cut(s) | 381 |
| HpySE526I | ACGT | 2 cut(s) | 306, 587 |
| Hsp92II | CATG | 3 cut(s) | 253, 397, 548 |
| Ksp22I | TGATCA | 2 cut(s) | 360, 541 |
| Kzo9I | GATC | 9 cut(s) | 155, 208, 218, 360, 405, 409, 471, 486, 541 |
| LmnI | GCTCC | 2 cut(s) | 258, 511 |
| MaeII | ACGT | 2 cut(s) | 306, 587 |
| MalI | GATC | 9 cut(s) | 157, 210, 220, 362, 407, 411, 473, 488, 543 |
| MboI | GATC | 9 cut(s) | 155, 208, 218, 360, 405, 409, 471, 486, 541 |
| MboII | GAAGA | 4 cut(s) | 225, 304, 607, 693 |
| MflI | RGATCY | 2 cut(s) | 155, 208 |
| MhlI | GDGCHC | 2 cut(s) | 8, 516 |
| MluCI | AATT | 3 cut(s) | 495, 505, 725 |
| MlyI | GAGTC | 1 cut(s) | 371 |
| MroXI | GAANNNNTTC | 1 cut(s) | 464 |
| MseI | TTAA | 1 cut(s) | 722 |
| MspI | CCGG | 4 cut(s) | 398, 442, 555, 592 |
| NdeII | GATC | 9 cut(s) | 155, 208, 218, 360, 405, 409, 471, 486, 541 |
| NlaIII | CATG | 3 cut(s) | 253, 397, 548 |
| NmeAIII | GCCGAG | 1 cut(s) | 240 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 363 |
| PdmI | GAANNNNTTC | 1 cut(s) | 464 |
| PfeI | GAWTC | 1 cut(s) | 182 |
| PinAI | ACCGGT | 1 cut(s) | 554 |
| PkrI | GCNGC | 1 cut(s) | 535 |
| PleI | GAGTC | 1 cut(s) | 371 |
| PpsI | GAGTC | 1 cut(s) | 371 |
| PsiI | TTATAA | 1 cut(s) | 733 |
| PspFI | CCCAGC | 1 cut(s) | 241 |
| PspPI | GGNCC | 1 cut(s) | 322 |
| PsuI | RGATCY | 2 cut(s) | 155, 208 |
| RsaI | GTAC | 1 cut(s) | 553 |
| RsaNI | GTAC | 1 cut(s) | 552 |
| SaqAI | TTAA | 1 cut(s) | 722 |
| SatI | GCNGC | 1 cut(s) | 534 |
| Sau3AI | GATC | 9 cut(s) | 155, 208, 218, 360, 405, 409, 471, 486, 541 |
| Sau96I | GGNCC | 1 cut(s) | 322 |
| SchI | GAGTC | 1 cut(s) | 371 |
| SduI | GDGCHC | 2 cut(s) | 8, 516 |
| SinI | GGWCC | 1 cut(s) | 322 |
| Sse9I | AATT | 3 cut(s) | 495, 505, 725 |
| SsiI | CCGC | 5 cut(s) | 59, 212, 517, 533, 619 |
| TaaI | ACNGT | 1 cut(s) | 331 |
| TaiI | ACGT | 2 cut(s) | 309, 590 |
| TaqI | TCGA | 3 cut(s) | 366, 408, 468 |
| TasI | AATT | 3 cut(s) | 495, 505, 725 |
| TauI | GCSGC | 1 cut(s) | 536 |
| TfiI | GAWTC | 1 cut(s) | 182 |
| Tru1I | TTAA | 1 cut(s) | 722 |
| Tru9I | TTAA | 1 cut(s) | 722 |
| TspDTI | ATGAA | 1 cut(s) | 225 |
| VpaK11BI | GGWCC | 1 cut(s) | 322 |
| XmnI | GAANNNNTTC | 1 cut(s) | 464 |