Rroxscaffold_4G00292690

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018535
Physical Location & Seq
Reverse (-)
12918572 .. 12921450
2879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00292690.1

Sequence Viewer

Length: 408 bp
ATGATCCTCAACCGCCGTAACCTCCGCAACAATCTCCGAGCCCTAACGAGTACATCCGCGGCGTCACGCTCCGATTCCTCTCGCCTATTGAACAAGGCCGAGATTGTCGAGCCGTTGATTCGGTCGATTCGGCGAATCCGGAGCACCGCCGCTCGTTCGTTTGACGGAACGCCGTCGCATAGTCATGTCCGTATTAGAGTAACCGGCAAGTGGGAGTGGCTTGAGGATGGGAAGCGCCTAAAGTTTTCTGAAGGCTTAGTCGATGTCCCAGGCACTGATGGGAAAGTTCTAAAAGAGAATGGCCGACCACGCGGTCGCCGGAATAAGCCCAAGTCTACACCTACTTTAGTGGTGGATGATGGGGGGGCGTCTTCGGAGGAGCTGAACGCCCTACCTCAAGAGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.98

Weight (kDa)

11.02

Isoelectric Point (pI)

62.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 312
AccBSI CCGCTC 1 cut(s) 152
AccI GTMKAC 1 cut(s) 335
AccII CGCG 2 cut(s) 59, 312
AccIII TCCGGA 1 cut(s) 138
AciI CCGC 7 cut(s) 13, 25, 57, 59, 147, 150, 312
AcoI YGGCCR 1 cut(s) 301
AcuI CTGAAG 1 cut(s) 270
AcyI GRCGYC 2 cut(s) 62, 368
AfaI GTAC 1 cut(s) 52
AfiI CCNNNNNNNGG 2 cut(s) 210, 401
AgsI TTSAA 1 cut(s) 91
AjnI CCWGG 1 cut(s) 268
AluBI AGCT 1 cut(s) 382
AluI AGCT 1 cut(s) 382
Alw21I GWGCWC 1 cut(s) 146
AlwNI CAGNNNCTG 1 cut(s) 275
Aor13HI TCCGGA 1 cut(s) 138
AoxI GGCC 2 cut(s) 96, 301
AspLEI GCGC 1 cut(s) 237
BanII GRGCYC 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 146
BccI CCATC 3 cut(s) 221, 272, 353
BceAI ACGGC 2 cut(s) 97, 157
BciT130I CCWGG 1 cut(s) 270
BfaI CTAG 1 cut(s) 406
BfoI RGCGCY 1 cut(s) 238
BisI GCNGC 2 cut(s) 60, 150
BlsI GCNGC 2 cut(s) 61, 151
Bme1390I CCNGG 1 cut(s) 270
BmrFI CCNGG 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 363
BpuEI CTTGAG 2 cut(s) 242, 381
BsaHI GRCGYC 2 cut(s) 62, 368
BsaJI CCNNGG 2 cut(s) 57, 268
BsaWI WCCGGW 1 cut(s) 138
Bsc4I CCNNNNNNNGG 2 cut(s) 210, 401
Bse118I RCCGGY 1 cut(s) 203
BseAI TCCGGA 1 cut(s) 138
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 2 cut(s) 57, 268
BseGI GGATG 3 cut(s) 53, 232, 361
BseLI CCNNNNNNNGG 2 cut(s) 210, 401
BseRI GAGGAG 1 cut(s) 392
Bsh1236I CGCG 2 cut(s) 59, 312
Bsh1285I CGRYCG 2 cut(s) 125, 316
BshFI GGCC 2 cut(s) 98, 303
BsiEI CGRYCG 2 cut(s) 125, 316
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 3 cut(s) 139, 204, 319
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 2 cut(s) 210, 401
BsmFI GGGAC 1 cut(s) 251
BsnI GGCC 2 cut(s) 98, 303
Bsp1286I GDGCHC 2 cut(s) 43, 146
Bsp13I TCCGGA 1 cut(s) 138
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 7 cut(s) 13, 25, 57, 59, 147, 150, 312
BspANI GGCC 2 cut(s) 98, 303
BspEI TCCGGA 1 cut(s) 138
BspFNI CGCG 2 cut(s) 59, 312
BsrBI CCGCTC 1 cut(s) 152
BsrFI RCCGGY 1 cut(s) 203
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 57, 268
BssMI GATC 1 cut(s) 3
BssNI GRCGYC 2 cut(s) 62, 368
Bst2UI CCWGG 1 cut(s) 270
BstACI GRCGYC 2 cut(s) 62, 368
BstDEI CTNAG 1 cut(s) 256
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 3 cut(s) 53, 232, 361
BstFNI CGCG 2 cut(s) 59, 312
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 1 cut(s) 6
BstMBI GATC 1 cut(s) 3
BstMCI CGRYCG 2 cut(s) 125, 316
BstMWI GCNNNNNNNGC 1 cut(s) 309
BstNI CCWGG 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 268
BstUI CGCG 2 cut(s) 59, 312
BstV2I GAAGAC 1 cut(s) 363
BsuRI GGCC 2 cut(s) 98, 303
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 3 cut(s) 53, 232, 361
BtsIMutI CAGTG 1 cut(s) 273
CaiI CAGNNNCTG 1 cut(s) 275
CfoI GCGC 1 cut(s) 237
Cfr10I RCCGGY 1 cut(s) 203
Cfr42I CCGCGG 1 cut(s) 60
CseI GACGC 2 cut(s) 51, 357
Csp6I GTAC 1 cut(s) 51
CviAII CATG 1 cut(s) 185
CviJI RGCY 9 cut(s) 41, 98, 112, 220, 255, 303, 328, 382, 405
CviKI_1 RGCY 9 cut(s) 41, 98, 112, 220, 255, 303, 328, 382, 405
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 1 cut(s) 256
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
DrdI GACNNNNNNGTC 1 cut(s) 312
DseDI GACNNNNNNGTC 1 cut(s) 312
EaeI YGGCCR 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 43
FaeI CATG 1 cut(s) 188
FaiI YATR 2 cut(s) 180, 186
FaqI GGGAC 1 cut(s) 251
FatI CATG 1 cut(s) 184
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 2 cut(s) 60, 150
FokI GGATG 3 cut(s) 40, 239, 368
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 2 cut(s) 60, 150
FspBI CTAG 1 cut(s) 406
GlaI GCGC 1 cut(s) 236
GluI GCNGC 2 cut(s) 60, 150
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 2 cut(s) 98, 303
HapII CCGG 3 cut(s) 139, 204, 319
HgaI GACGC 2 cut(s) 51, 357
HhaI GCGC 1 cut(s) 237
Hin1I GRCGYC 2 cut(s) 62, 368
Hin1II CATG 1 cut(s) 188
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HinfI GANTC 4 cut(s) 74, 118, 127, 135
HpaII CCGG 3 cut(s) 139, 204, 319
Hpy166II GTNNAC 1 cut(s) 336
Hpy188I TCNGA 4 cut(s) 38, 73, 250, 376
Hpy188III TCNNGA 2 cut(s) 139, 398
Hpy8I GTNNAC 1 cut(s) 336
Hpy99I CGWCG 1 cut(s) 178
HpyAV CCTTC 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 309
HpyF3I CTNAG 1 cut(s) 256
Hsp92I GRCGYC 2 cut(s) 62, 368
Hsp92II CATG 1 cut(s) 188
HspAI GCGC 1 cut(s) 235
Kpn2I TCCGGA 1 cut(s) 138
KspI CCGCGG 1 cut(s) 60
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 3 cut(s) 74, 141, 379
LpnPI CCDG 5 cut(s) 152, 217, 255, 282, 332
MaeI CTAG 1 cut(s) 406
MaeIII GTNAC 3 cut(s) 17, 63, 199
MalI GATC 1 cut(s) 5
MbiI CCGCTC 1 cut(s) 152
MboI GATC 1 cut(s) 3
MboII GAAGA 1 cut(s) 363
MhlI GDGCHC 2 cut(s) 43, 146
MnlI CCTC 7 cut(s) 17, 32, 88, 217, 370, 394, 405
MroI TCCGGA 1 cut(s) 138
MslI CAYNNNNRTG 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 3 cut(s) 139, 204, 319
MspR9I CCNGG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 270
MvnI CGCG 2 cut(s) 59, 312
MwoI GCNNNNNNNGC 1 cut(s) 309
NdeII GATC 1 cut(s) 3
NlaIII CATG 1 cut(s) 188
NmeAIII GCCGAG 1 cut(s) 124
NmuCI GTSAC 1 cut(s) 63
PfeI GAWTC 4 cut(s) 74, 118, 127, 135
PkrI GCNGC 2 cut(s) 61, 151
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 275
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
RseI CAYNNNNRTG 1 cut(s) 183
SacII CCGCGG 1 cut(s) 60
SatI GCNGC 2 cut(s) 60, 150
Sau3AI GATC 1 cut(s) 3
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 2 cut(s) 43, 146
SetI ASST 4 cut(s) 24, 343, 384, 397
Sfr303I CCGCGG 1 cut(s) 60
SgrBI CCGCGG 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 2 cut(s) 221, 396
SmoI CTYRAG 2 cut(s) 221, 396
SsiI CCGC 7 cut(s) 13, 25, 57, 59, 147, 150, 312
SspMI CTAG 1 cut(s) 406
StyD4I CCNGG 1 cut(s) 268
TaqI TCGA 3 cut(s) 108, 125, 261
TaqII GACCGA 1 cut(s) 111
TatI WGTACW 1 cut(s) 50
TauI GCSGC 2 cut(s) 62, 152
TfiI GAWTC 4 cut(s) 74, 118, 127, 135
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 1 cut(s) 63
Tsp45I GTSAC 1 cut(s) 63
TspGWI ACGGA 2 cut(s) 179, 180
TspRI CASTG 1 cut(s) 280
XmiI GTMKAC 1 cut(s) 335
XspI CTAG 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.