The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
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| Species | Orthologous Gene IDs |
|---|---|
| pyrus_communis | pycom07g07040 |
| rosa_multiflora | Rmu_sc0001136.1_g000002 Rmu_sc0001437.1_g000003 Rmu_sc0005385.1_g000021 Rmu_sc0010640.1_g000008 |
| rosa_roxburghii | Rroxscaffold_4G00292690 Rroxscaffold_5G00356490 |
| rosa_samantha | Rh5DG227500 Rh6AG009200 Rh6AG111800 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 95 |
| AciI | CCGC | 1 cut(s) | 67 |
| AclWI | GGATC | 1 cut(s) | 166 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 85 |
| AluBI | AGCT | 2 cut(s) | 49, 106 |
| AluI | AGCT | 2 cut(s) | 49, 106 |
| AlwI | GGATC | 1 cut(s) | 166 |
| Ama87I | CYCGRG | 2 cut(s) | 122, 147 |
| AspS9I | GGNCC | 1 cut(s) | 88 |
| AsuC2I | CCSGG | 1 cut(s) | 164 |
| AvaI | CYCGRG | 2 cut(s) | 122, 147 |
| AvaII | GGWCC | 1 cut(s) | 88 |
| BceAI | ACGGC | 1 cut(s) | 46 |
| BcnI | CCSGG | 1 cut(s) | 164 |
| BfaI | CTAG | 1 cut(s) | 184 |
| Bme1390I | CCNGG | 1 cut(s) | 164 |
| Bme18I | GGWCC | 1 cut(s) | 88 |
| BmeT110I | CYCGRG | 2 cut(s) | 122, 147 |
| BmgT120I | GGNCC | 1 cut(s) | 88 |
| BmrFI | CCNGG | 1 cut(s) | 164 |
| BpuMI | CCSGG | 1 cut(s) | 164 |
| BsaJI | CCNNGG | 1 cut(s) | 148 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 85 |
| BseDI | CCNNGG | 1 cut(s) | 148 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 85 |
| BseRI | GAGGAG | 2 cut(s) | 101, 104 |
| BseYI | CCCAGC | 1 cut(s) | 45 |
| BsiHKCI | CYCGRG | 2 cut(s) | 122, 147 |
| BsiSI | CCGG | 1 cut(s) | 164 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 85 |
| BsoBI | CYCGRG | 2 cut(s) | 122, 147 |
| Bsp143I | GATC | 3 cut(s) | 18, 126, 171 |
| BspACI | CCGC | 1 cut(s) | 67 |
| BspHI | TCATGA | 1 cut(s) | 15 |
| BspPI | GGATC | 1 cut(s) | 166 |
| BspQI | GCTCTTC | 1 cut(s) | 56 |
| BssECI | CCNNGG | 1 cut(s) | 148 |
| BssMI | GATC | 3 cut(s) | 18, 126, 171 |
| Bst4CI | ACNGT | 1 cut(s) | 97 |
| Bst6I | CTCTTC | 1 cut(s) | 56 |
| BstKTI | GATC | 3 cut(s) | 21, 129, 174 |
| BstMBI | GATC | 3 cut(s) | 18, 126, 171 |
| BstSCI | CCNGG | 1 cut(s) | 162 |
| CciI | TCATGA | 1 cut(s) | 15 |
| Cfr13I | GGNCC | 1 cut(s) | 88 |
| CviAII | CATG | 1 cut(s) | 16 |
| CviJI | RGCY | 2 cut(s) | 49, 106 |
| CviKI_1 | RGCY | 2 cut(s) | 49, 106 |
| DpnI | GATC | 3 cut(s) | 20, 128, 173 |
| DpnII | GATC | 3 cut(s) | 18, 126, 171 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 95 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 95 |
| Eam1104I | CTCTTC | 1 cut(s) | 56 |
| EarI | CTCTTC | 1 cut(s) | 56 |
| Eco47I | GGWCC | 1 cut(s) | 88 |
| Eco88I | CYCGRG | 2 cut(s) | 122, 147 |
| FaeI | CATG | 1 cut(s) | 19 |
| FaiI | YATR | 2 cut(s) | 17, 38 |
| FatI | CATG | 1 cut(s) | 15 |
| FspBI | CTAG | 1 cut(s) | 184 |
| GsaI | CCCAGC | 1 cut(s) | 49 |
| HapII | CCGG | 1 cut(s) | 164 |
| Hin1II | CATG | 1 cut(s) | 19 |
| HindIII | AAGCTT | 1 cut(s) | 104 |
| HinfI | GANTC | 1 cut(s) | 143 |
| HpaII | CCGG | 1 cut(s) | 164 |
| Hpy188III | TCNNGA | 4 cut(s) | 16, 100, 124, 147 |
| HpyAV | CCTTC | 1 cut(s) | 91 |
| HpyCH4III | ACNGT | 1 cut(s) | 97 |
| HpyCH4IV | ACGT | 1 cut(s) | 72 |
| HpySE526I | ACGT | 1 cut(s) | 72 |
| Hsp92II | CATG | 1 cut(s) | 19 |
| Kzo9I | GATC | 3 cut(s) | 18, 126, 171 |
| LguI | GCTCTTC | 1 cut(s) | 56 |
| LpnPI | CCDG | 3 cut(s) | 59, 113, 177 |
| MaeI | CTAG | 1 cut(s) | 184 |
| MaeII | ACGT | 1 cut(s) | 72 |
| MalI | GATC | 3 cut(s) | 20, 128, 173 |
| MboI | GATC | 3 cut(s) | 18, 126, 171 |
| MboII | GAAGA | 2 cut(s) | 16, 43 |
| MluCI | AATT | 2 cut(s) | 39, 60 |
| MnlI | CCTC | 7 cut(s) | 79, 86, 122, 125, 131, 143, 185 |
| MseI | TTAA | 1 cut(s) | 132 |
| MspI | CCGG | 1 cut(s) | 164 |
| MspR9I | CCNGG | 1 cut(s) | 164 |
| NciI | CCSGG | 1 cut(s) | 164 |
| NdeII | GATC | 3 cut(s) | 18, 126, 171 |
| NlaIII | CATG | 1 cut(s) | 19 |
| PaeR7I | CTCGAG | 1 cut(s) | 122 |
| PagI | TCATGA | 1 cut(s) | 15 |
| PciSI | GCTCTTC | 1 cut(s) | 56 |
| PfeI | GAWTC | 1 cut(s) | 143 |
| PfoI | TCCNGGA | 1 cut(s) | 162 |
| PspFI | CCCAGC | 1 cut(s) | 45 |
| PspPI | GGNCC | 1 cut(s) | 88 |
| SapI | GCTCTTC | 1 cut(s) | 56 |
| SaqAI | TTAA | 1 cut(s) | 132 |
| Sau3AI | GATC | 3 cut(s) | 18, 126, 171 |
| Sau96I | GGNCC | 1 cut(s) | 88 |
| ScrFI | CCNGG | 1 cut(s) | 164 |
| SetI | ASST | 4 cut(s) | 51, 75, 108, 123 |
| Sfr274I | CTCGAG | 1 cut(s) | 122 |
| SinI | GGWCC | 1 cut(s) | 88 |
| SlaI | CTCGAG | 1 cut(s) | 122 |
| SmlI | CTYRAG | 1 cut(s) | 122 |
| SmoI | CTYRAG | 1 cut(s) | 122 |
| Sse9I | AATT | 2 cut(s) | 39, 60 |
| SsiI | CCGC | 1 cut(s) | 67 |
| SspMI | CTAG | 1 cut(s) | 184 |
| StyD4I | CCNGG | 1 cut(s) | 162 |
| TaaI | ACNGT | 1 cut(s) | 97 |
| TaiI | ACGT | 1 cut(s) | 75 |
| TaqI | TCGA | 2 cut(s) | 84, 123 |
| TasI | AATT | 2 cut(s) | 39, 60 |
| TfiI | GAWTC | 1 cut(s) | 143 |
| Tru1I | TTAA | 1 cut(s) | 132 |
| Tru9I | TTAA | 1 cut(s) | 132 |
| TspDTI | ATGAA | 2 cut(s) | 17, 43 |
| VpaK11BI | GGWCC | 1 cut(s) | 88 |
| XhoI | CTCGAG | 1 cut(s) | 122 |
| XspI | CTAG | 1 cut(s) | 184 |