Rh6AG111800

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0018535
Physical Location & Seq
Reverse (-)
16639731 .. 16640048
318 bp
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UTR
Exon/CDS
Intron
Rh6AG111800.1

Sequence Viewer

Length: 318 bp
ATGATCTTGCTCAATGGCGACACAATTCCCCAGCTCTTCATCACCATTATCCGCTACGTCCTCCCCTCCGAGGACCACACCGTCCAGAAGCTTCTCCTCCTATACCTTGAGATCATTGACAAGACATATTCCCGAGGCAAAGTTCTCTCGGAGATGATCCTCAACTGCCAGAACCTCCGCAACAATCTCCAGAGCCCTAACGAGTATATTCGCGGCATCACGCTTCGATTCCTCTGCCGATTGAACGAGGCCGAGATCGTCGAGCCGTTGATTCCGTCGATTCTGGCGAATCTGGAGCATCACCACCCGTTCATTTGA

Protein Analysis

105

Amino Acids

12.22

Weight (kDa)

6.04

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 2 - 105 9.1e-19 Adaptin N terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
AccII CGCG 1 cut(s) 213
AciI CCGC 3 cut(s) 52, 178, 213
AclWI GGATC 1 cut(s) 151
AfiI CCNNNNNNNGG 1 cut(s) 70
AgsI TTSAA 1 cut(s) 244
AluBI AGCT 2 cut(s) 34, 91
AluI AGCT 2 cut(s) 34, 91
AlwI GGATC 1 cut(s) 151
Ama87I CYCGRG 1 cut(s) 132
AoxI GGCC 1 cut(s) 249
AspS9I GGNCC 1 cut(s) 73
AsuHPI GGTGA 2 cut(s) 34, 293
AvaI CYCGRG 1 cut(s) 132
AvaII GGWCC 1 cut(s) 73
BanII GRGCYC 1 cut(s) 197
BceAI ACGGC 1 cut(s) 250
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BisI GCNGC 1 cut(s) 214
BlsI GCNGC 1 cut(s) 215
Bme18I GGWCC 1 cut(s) 73
BmeT110I CYCGRG 1 cut(s) 132
BmgT120I GGNCC 1 cut(s) 73
BmsI GCATC 2 cut(s) 225, 307
BpmI CTGGAG 2 cut(s) 173, 314
BpuEI CTTGAG 1 cut(s) 128
BsaJI CCNNGG 2 cut(s) 69, 133
Bsc4I CCNNNNNNNGG 1 cut(s) 70
BseDI CCNNGG 2 cut(s) 69, 133
BseLI CCNNNNNNNGG 1 cut(s) 70
BseRI GAGGAG 1 cut(s) 86
BseYI CCCAGC 1 cut(s) 30
Bsh1236I CGCG 1 cut(s) 213
BshFI GGCC 1 cut(s) 251
BsiHKCI CYCGRG 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 70
BsnI GGCC 1 cut(s) 251
BsoBI CYCGRG 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 197
Bsp143I GATC 4 cut(s) 3, 111, 156, 255
BspACI CCGC 3 cut(s) 52, 178, 213
BspANI GGCC 1 cut(s) 251
BspFNI CGCG 1 cut(s) 213
BspPI GGATC 1 cut(s) 151
BspQI GCTCTTC 1 cut(s) 41
BssECI CCNNGG 2 cut(s) 69, 133
BssMI GATC 4 cut(s) 3, 111, 156, 255
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 1 cut(s) 41
BstFNI CGCG 1 cut(s) 213
BstKTI GATC 4 cut(s) 6, 114, 159, 258
BstMBI GATC 4 cut(s) 3, 111, 156, 255
BstUI CGCG 1 cut(s) 213
BsuRI GGCC 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 73
CviJI RGCY 5 cut(s) 34, 91, 195, 251, 265
CviKI_1 RGCY 5 cut(s) 34, 91, 195, 251, 265
DpnI GATC 4 cut(s) 5, 113, 158, 257
DpnII GATC 4 cut(s) 3, 111, 156, 255
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco24I GRGCYC 1 cut(s) 197
Eco47I GGWCC 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 197
FaiI YATR 3 cut(s) 103, 127, 207
Fnu4HI GCNGC 1 cut(s) 214
FriOI GRGCYC 1 cut(s) 197
Fsp4HI GCNGC 1 cut(s) 214
GluI GCNGC 1 cut(s) 214
GsaI CCCAGC 1 cut(s) 34
GsuI CTGGAG 2 cut(s) 173, 314
HaeIII GGCC 1 cut(s) 251
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 4 cut(s) 228, 271, 280, 289
HphI GGTGA 2 cut(s) 34, 293
Hpy188I TCNGA 2 cut(s) 70, 151
Hpy188III TCNNGA 4 cut(s) 85, 132, 190, 293
Hpy99I CGWCG 2 cut(s) 263, 280
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 57
HpySE526I ACGT 1 cut(s) 57
Kzo9I GATC 4 cut(s) 3, 111, 156, 255
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 1 cut(s) 295
LpnPI CCDG 6 cut(s) 44, 98, 182, 203, 269, 278
LweI GCATC 2 cut(s) 225, 307
MaeII ACGT 1 cut(s) 57
MalI GATC 4 cut(s) 5, 113, 158, 257
MboI GATC 4 cut(s) 3, 111, 156, 255
MboII GAAGA 1 cut(s) 28
MhlI GDGCHC 1 cut(s) 197
MluCI AATT 1 cut(s) 24
MnlI CCTC 9 cut(s) 64, 71, 76, 107, 128, 170, 185, 241, 242
MvnI CGCG 1 cut(s) 213
NdeII GATC 4 cut(s) 3, 111, 156, 255
NmeAIII GCCGAG 1 cut(s) 277
PciSI GCTCTTC 1 cut(s) 41
PcsI WCGNNNNNNNCGW 1 cut(s) 284
PfeI GAWTC 4 cut(s) 228, 271, 280, 289
PkrI GCNGC 1 cut(s) 215
PspFI CCCAGC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 73
SapI GCTCTTC 1 cut(s) 41
SatI GCNGC 1 cut(s) 214
Sau3AI GATC 4 cut(s) 3, 111, 156, 255
Sau96I GGNCC 1 cut(s) 73
SduI GDGCHC 1 cut(s) 197
SetI ASST 5 cut(s) 36, 60, 93, 108, 177
SfaNI GCATC 2 cut(s) 225, 307
SinI GGWCC 1 cut(s) 73
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
Sse9I AATT 1 cut(s) 24
SsiI CCGC 3 cut(s) 52, 178, 213
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 60
TaqI TCGA 3 cut(s) 226, 261, 278
TasI AATT 1 cut(s) 24
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 4 cut(s) 228, 271, 280, 289
TspDTI ATGAA 2 cut(s) 28, 301
TspGWI ACGGA 1 cut(s) 264
VpaK11BI GGWCC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.