Rh5DG227500

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
Physical Location & Seq
Forward (+)
25650015 .. 25650200
186 bp
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UTR
Exon/CDS
Intron
Rh5DG227500.1

Sequence Viewer

Length: 186 bp
ATGAAGAAGGCAATCATGATCTTGCTCAACGGTGACACAATTCCCCAACTCTTCATCACCATTATCCGCTACGTCCTCCCCTCCGAGGACCACACCGTCCAGAAGCTTCTCCTCCTCTACCTCGAGATCATTAACAAGACAGATTCCCGAGGCAAAGTTCTCCCGGAGATGATCCTCAACTGCTAG

Protein Analysis

61

Amino Acids

7.03

Weight (kDa)

7.89

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 1 - 49 6.2e-06 Adaptin N terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
AciI CCGC 1 cut(s) 67
AclWI GGATC 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 85
AluBI AGCT 1 cut(s) 106
AluI AGCT 1 cut(s) 106
AlwI GGATC 1 cut(s) 166
Ama87I CYCGRG 2 cut(s) 122, 147
AspS9I GGNCC 1 cut(s) 88
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 2 cut(s) 44, 49
AvaI CYCGRG 2 cut(s) 122, 147
AvaII GGWCC 1 cut(s) 88
BcnI CCSGG 1 cut(s) 164
BfaI CTAG 1 cut(s) 184
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 1 cut(s) 88
BmeT110I CYCGRG 2 cut(s) 122, 147
BmgT120I GGNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 84, 148
Bsc4I CCNNNNNNNGG 1 cut(s) 85
BseDI CCNNGG 2 cut(s) 84, 148
BseLI CCNNNNNNNGG 1 cut(s) 85
BseRI GAGGAG 2 cut(s) 101, 104
BsiHKCI CYCGRG 2 cut(s) 122, 147
BsiSI CCGG 1 cut(s) 164
BslI CCNNNNNNNGG 1 cut(s) 85
BsoBI CYCGRG 2 cut(s) 122, 147
Bsp143I GATC 3 cut(s) 18, 126, 171
BspACI CCGC 1 cut(s) 67
BspHI TCATGA 1 cut(s) 15
BspPI GGATC 1 cut(s) 166
BssECI CCNNGG 2 cut(s) 84, 148
BssMI GATC 3 cut(s) 18, 126, 171
Bst4CI ACNGT 2 cut(s) 32, 97
Bst6I CTCTTC 1 cut(s) 56
BstKTI GATC 3 cut(s) 21, 129, 174
BstMBI GATC 3 cut(s) 18, 126, 171
BstSCI CCNGG 1 cut(s) 162
CciI TCATGA 1 cut(s) 15
Cfr13I GGNCC 1 cut(s) 88
CviAII CATG 1 cut(s) 16
CviJI RGCY 1 cut(s) 106
CviKI_1 RGCY 1 cut(s) 106
DpnI GATC 3 cut(s) 20, 128, 173
DpnII GATC 3 cut(s) 18, 126, 171
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco47I GGWCC 1 cut(s) 88
Eco88I CYCGRG 2 cut(s) 122, 147
FaeI CATG 1 cut(s) 19
FaiI YATR 1 cut(s) 17
FatI CATG 1 cut(s) 15
FspBI CTAG 1 cut(s) 184
HapII CCGG 1 cut(s) 164
Hin1II CATG 1 cut(s) 19
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 1 cut(s) 143
HpaII CCGG 1 cut(s) 164
HphI GGTGA 2 cut(s) 44, 49
Hpy188I TCNGA 1 cut(s) 85
Hpy188III TCNNGA 4 cut(s) 16, 100, 124, 147
HpyCH4III ACNGT 2 cut(s) 32, 97
HpyCH4IV ACGT 1 cut(s) 72
HpySE526I ACGT 1 cut(s) 72
Hsp92II CATG 1 cut(s) 19
Kzo9I GATC 3 cut(s) 18, 126, 171
LpnPI CCDG 2 cut(s) 113, 177
MaeI CTAG 1 cut(s) 184
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 32
MalI GATC 3 cut(s) 20, 128, 173
MboI GATC 3 cut(s) 18, 126, 171
MboII GAAGA 2 cut(s) 16, 43
MluCI AATT 1 cut(s) 39
MnlI CCTC 8 cut(s) 79, 86, 91, 122, 125, 131, 143, 185
MseI TTAA 1 cut(s) 132
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 164
NciI CCSGG 1 cut(s) 164
NdeII GATC 3 cut(s) 18, 126, 171
NlaIII CATG 1 cut(s) 19
NmuCI GTSAC 1 cut(s) 32
PaeR7I CTCGAG 1 cut(s) 122
PagI TCATGA 1 cut(s) 15
PfeI GAWTC 1 cut(s) 143
PfoI TCCNGGA 1 cut(s) 162
PspPI GGNCC 1 cut(s) 88
SaqAI TTAA 1 cut(s) 132
Sau3AI GATC 3 cut(s) 18, 126, 171
Sau96I GGNCC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 164
SetI ASST 3 cut(s) 75, 108, 123
Sfr274I CTCGAG 1 cut(s) 122
SinI GGWCC 1 cut(s) 88
SlaI CTCGAG 1 cut(s) 122
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 1 cut(s) 39
SsiI CCGC 1 cut(s) 67
SspMI CTAG 1 cut(s) 184
StyD4I CCNGG 1 cut(s) 162
TaaI ACNGT 2 cut(s) 32, 97
TaiI ACGT 1 cut(s) 75
TaqI TCGA 1 cut(s) 123
TasI AATT 1 cut(s) 39
TfiI GAWTC 1 cut(s) 143
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TseFI GTSAC 1 cut(s) 32
Tsp45I GTSAC 1 cut(s) 32
TspDTI ATGAA 2 cut(s) 17, 43
VpaK11BI GGWCC 1 cut(s) 88
XhoI CTCGAG 1 cut(s) 122
XspI CTAG 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.