pycom07g09300

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009548
Physical Location & Seq
Forward (+)
8552671 .. 8558419
5749 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1035 bp
ATGGATTCGCGCAGCAACAAAGAAGCAGTTCAAGCAACAAACGACGACGCTTCGGCCAGCAAACTGTCGTGCGTGAAGAAAGGGTACATGAAAGACGATTATGTCCATTTGTTTGTGAGAAGACCTGTGAGAAGATCTCCGATTATTAACCGCGGTTACTTTGCTCGTTGGGCTGCTCTGCGGAAGCTTTTGAATCAGTTTCTTGATACCGAAGGAAATGCGGGTGAAAAGGGTCATTTGAAGAAGCAGATATTATCACTTGGAGCTGGCTTTGATACAACATATTTTCAGCTGCAGGATGAGGGGAAAGCACCATATTTGTATGTTGAGCTGGATTTTAAGGAGGTAACTAGTAAGAAGGCCACGCTTATTGAAACGTGCAGTCATTTGAGGGACAAAATTAGTGCGACAGCATCAATCTCTCGAGAGAGTGGAGAAGTGCTCAGTGACCACTACAAGCTACTTCCTGTTGATTTGCGTGACATACCAAAATTAGCCGATGTTATAACTTTAGCTGGTATGGATCCGAGCCTTCCAACTTTTATAATTGCAGAATGCGTTTTGATATACTTGGATCCAGATTCAAGTCGTTCCATAGTTAGATGGACATCAAAAACATTTTCAACGGCGATATTTTTCTTATATGAGCAGATTCATCCAGATGATGCTTTTGGGCAGCAGATGATCGATCATCAAGAAACTTGGAGAGTCGAGGCTGTGCACTCTTGGGCATACATGATACACCAACATTACAGGCAAAGGAAAACCTTTTTGTTGAACAAGGATGGCAGGGCCGTTGCCTGGGACATGCTTAAAGTATACAGTGATTTTGTTGAAGCTCAAGAAAGACGCAGGATTGAACGATTGGAATTGTTTGATGAATTTGAAGAATGGCATATGATGCAGGAGCACTACTGCGTGGCTTATGCAATCAATGATGCACTGGGTCTGTTTGGTGACTTCGGTTTTCTTAACCACCGCCGCCAGCAGCCGCAGCCACACGTGCTGAACCCCTCCTCATCAGCATCACCATGA

Protein Analysis

345

Amino Acids

39.72

Weight (kDa)

6.36

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 39 - 202 2.9e-20 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 506, 545
AasI GACNNNNNNGTC 1 cut(s) 101
AccI GTMKAC 1 cut(s) 819
AccII CGCG 2 cut(s) 10, 153
AciI CCGC 7 cut(s) 151, 153, 181, 221, 979, 982, 992
AclWI GGATC 4 cut(s) 518, 531, 569, 582
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 881
AcvI CACGTG 1 cut(s) 1003
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 801
AflIII ACRYGT 1 cut(s) 1000
AhlI ACTAGT 1 cut(s) 350
AjnI CCWGG 1 cut(s) 800
AluBI AGCT 7 cut(s) 187, 266, 292, 331, 460, 515, 839
AluI AGCT 7 cut(s) 187, 266, 292, 331, 460, 515, 839
Alw21I GWGCWC 3 cut(s) 444, 723, 912
Alw44I GTGCAC 1 cut(s) 719
AlwI GGATC 4 cut(s) 518, 531, 569, 582
Ama87I CYCGRG 1 cut(s) 423
AoxI GGCC 3 cut(s) 54, 360, 792
ApaLI GTGCAC 1 cut(s) 719
ApeKI GCWGC 6 cut(s) 12, 173, 292, 676, 988, 994
ApoI RAATTY 1 cut(s) 881
Asp700I GAANNNNTTC 1 cut(s) 27
AspLEI GCGC 1 cut(s) 12
AspS9I GGNCC 1 cut(s) 792
AsuHPI GGTGA 3 cut(s) 236, 968, 1020
AvaI CYCGRG 1 cut(s) 423
BaeGI GKGCMC 1 cut(s) 723
BamHI GGATCC 2 cut(s) 523, 574
BarI GAAGNNNNNNTAC 2 cut(s) 68, 100
BbrPI CACGTG 1 cut(s) 1003
BbsI GAAGAC 1 cut(s) 127
Bbv12I GWGCWC 3 cut(s) 444, 723, 912
BbvI GCAGC 6 cut(s) 24, 160, 279, 688, 1000, 1006
BccI CCATC 2 cut(s) 597, 779
BceAI ACGGC 2 cut(s) 642, 779
BcgI CGANNNNNNTGC 2 cut(s) 200, 234
BciT130I CCWGG 1 cut(s) 802
BcuI ACTAGT 1 cut(s) 350
BfaI CTAG 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 293
BglII AGATCT 1 cut(s) 134
BisI GCNGC 8 cut(s) 13, 174, 293, 677, 982, 989, 992, 995
BlsI GCNGC 8 cut(s) 14, 175, 294, 678, 983, 990, 993, 996
Bme1390I CCNGG 1 cut(s) 802
BmeT110I CYCGRG 1 cut(s) 423
BmgT120I GGNCC 1 cut(s) 792
BmiI GGNNCC 2 cut(s) 525, 576
BmrFI CCNGG 1 cut(s) 802
BmrI ACTGGG 1 cut(s) 953
BmsI GCATC 4 cut(s) 422, 655, 891, 928
BmuI ACTGGG 1 cut(s) 953
BpiI GAAGAC 1 cut(s) 127
BplI GAGNNNNNCTC 4 cut(s) 121, 153, 426, 458
BpuEI CTTGAG 1 cut(s) 825
Bsa29I ATCGAT 1 cut(s) 687
BsaAI YACGTR 1 cut(s) 1003
BsaBI GATNNNNATC 1 cut(s) 607
BsaJI CCNNGG 2 cut(s) 151, 801
Bsc4I CCNNNNNNNGG 1 cut(s) 801
Bse1I ACTGG 1 cut(s) 948
Bse8I GATNNNNATC 1 cut(s) 607
BseBI CCWGG 1 cut(s) 802
BseCI ATCGAT 1 cut(s) 687
BseDI CCNNGG 2 cut(s) 151, 801
BseGI GGATG 3 cut(s) 304, 655, 790
BseJI GATNNNNATC 1 cut(s) 607
BseLI CCNNNNNNNGG 1 cut(s) 801
BseMII CTCAG 1 cut(s) 457
BseNI ACTGG 1 cut(s) 948
BseRI GAGGAG 1 cut(s) 1006
BseSI GKGCMC 1 cut(s) 723
BseXI GCAGC 6 cut(s) 24, 160, 279, 688, 1000, 1006
BsgI GTGCAG 1 cut(s) 400
Bsh1236I CGCG 2 cut(s) 10, 153
BshFI GGCC 3 cut(s) 56, 362, 794
BshVI ATCGAT 1 cut(s) 687
BsiHKAI GWGCWC 3 cut(s) 444, 723, 912
BsiHKCI CYCGRG 1 cut(s) 423
BslFI GGGAC 2 cut(s) 407, 818
BslI CCNNNNNNNGG 1 cut(s) 801
BsmFI GGGAC 2 cut(s) 407, 818
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 3 cut(s) 56, 362, 794
BsoBI CYCGRG 1 cut(s) 423
Bsp1286I GDGCHC 3 cut(s) 444, 723, 912
Bsp143I GATC 5 cut(s) 134, 523, 574, 684, 688
BspACI CCGC 7 cut(s) 151, 153, 181, 221, 979, 982, 992
BspANI GGCC 3 cut(s) 56, 362, 794
BspCNI CTCAG 1 cut(s) 456
BspDI ATCGAT 1 cut(s) 687
BspFNI CGCG 2 cut(s) 10, 153
BspLI GGNNCC 2 cut(s) 525, 576
BspMAI CTGCAG 1 cut(s) 297
BspPI GGATC 4 cut(s) 518, 531, 569, 582
BsrI ACTGG 1 cut(s) 948
BssECI CCNNGG 2 cut(s) 151, 801
BssMI GATC 5 cut(s) 134, 523, 574, 684, 688
BssNAI GTATAC 1 cut(s) 820
Bst1107I GTATAC 1 cut(s) 820
Bst2UI CCWGG 1 cut(s) 802
Bst4CI ACNGT 2 cut(s) 66, 824
BstAPI GCANNNNNTGC 1 cut(s) 901
BstBAI YACGTR 1 cut(s) 1003
BstC8I GCNNGC 3 cut(s) 58, 268, 986
BstDEI CTNAG 1 cut(s) 443
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 3 cut(s) 304, 655, 790
BstFNI CGCG 2 cut(s) 10, 153
BstHHI GCGC 1 cut(s) 12
BstKTI GATC 5 cut(s) 137, 526, 577, 687, 691
BstMBI GATC 5 cut(s) 134, 523, 574, 684, 688
BstMWI GCNNNNNNNGC 5 cut(s) 32, 170, 901, 994, 1003
BstNI CCWGG 1 cut(s) 802
BstNSI RCATGY 1 cut(s) 811
BstSCI CCNGG 1 cut(s) 800
BstSFI CTRYAG 1 cut(s) 293
BstSLI GKGCMC 1 cut(s) 723
BstUI CGCG 2 cut(s) 10, 153
BstV1I GCAGC 6 cut(s) 24, 160, 279, 688, 1000, 1006
BstV2I GAAGAC 1 cut(s) 127
BstX2I RGATCY 3 cut(s) 134, 523, 574
BstYI RGATCY 3 cut(s) 134, 523, 574
BstZ17I GTATAC 1 cut(s) 820
Bsu15I ATCGAT 1 cut(s) 687
BsuRI GGCC 3 cut(s) 56, 362, 794
BsuTUI ATCGAT 1 cut(s) 687
BtgI CCRYGG 1 cut(s) 151
BtsCI GGATG 3 cut(s) 304, 655, 790
BtsIMutI CAGTG 3 cut(s) 451, 829, 941
Cac8I GCNNGC 3 cut(s) 58, 268, 986
CfoI GCGC 1 cut(s) 12
Cfr13I GGNCC 1 cut(s) 792
Cfr42I CCGCGG 1 cut(s) 154
ClaI ATCGAT 1 cut(s) 687
CseI GACGC 2 cut(s) 56, 858
Csp6I GTAC 1 cut(s) 85
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 4 cut(s) 88, 736, 808, 1032
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 443
DpnI GATC 5 cut(s) 136, 525, 576, 686, 690
DpnII GATC 5 cut(s) 134, 523, 574, 684, 688
DrdI GACNNNNNNGTC 1 cut(s) 101
DseDI GACNNNNNNGTC 1 cut(s) 101
EaeI YGGCCR 1 cut(s) 54
Eco72I CACGTG 1 cut(s) 1003
Eco88I CYCGRG 1 cut(s) 423
EcoRII CCWGG 1 cut(s) 800
FaeI CATG 4 cut(s) 91, 739, 811, 1035
FaqI GGGAC 2 cut(s) 407, 818
FatI CATG 4 cut(s) 87, 735, 807, 1031
FauI CCCGC 1 cut(s) 214
FauNDI CATATG 1 cut(s) 897
FblI GTMKAC 1 cut(s) 819
Fnu4HI GCNGC 8 cut(s) 13, 174, 293, 677, 982, 989, 992, 995
FokI GGATG 3 cut(s) 311, 642, 797
Fsp4HI GCNGC 8 cut(s) 13, 174, 293, 677, 982, 989, 992, 995
FspBI CTAG 1 cut(s) 351
GlaI GCGC 1 cut(s) 11
GluI GCNGC 8 cut(s) 13, 174, 293, 677, 982, 989, 992, 995
HaeIII GGCC 3 cut(s) 56, 362, 794
HgaI GACGC 2 cut(s) 56, 858
HhaI GCGC 1 cut(s) 12
Hin1II CATG 4 cut(s) 91, 739, 811, 1035
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 5 cut(s) 5, 193, 581, 652, 708
HphI GGTGA 3 cut(s) 236, 968, 1020
Hpy166II GTNNAC 2 cut(s) 721, 820
Hpy188I TCNGA 2 cut(s) 141, 528
Hpy188III TCNNGA 7 cut(s) 203, 423, 425, 578, 659, 695, 842
Hpy8I GTNNAC 2 cut(s) 721, 820
Hpy99I CGWCG 2 cut(s) 47, 50
HpyAV CCTTC 3 cut(s) 206, 352, 542
HpyCH4III ACNGT 2 cut(s) 66, 824
HpyCH4IV ACGT 2 cut(s) 377, 1002
HpyCH4V TGCA 7 cut(s) 295, 381, 551, 721, 904, 929, 941
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 170, 901, 994, 1003
HpyF3I CTNAG 1 cut(s) 443
HpySE526I ACGT 2 cut(s) 377, 1002
Hsp92II CATG 4 cut(s) 91, 739, 811, 1035
HspAI GCGC 1 cut(s) 10
KspI CCGCGG 1 cut(s) 154
Kzo9I GATC 5 cut(s) 134, 523, 574, 684, 688
LmnI GCTCC 2 cut(s) 263, 907
Lsp1109I GCAGC 6 cut(s) 24, 160, 279, 688, 1000, 1006
LweI GCATC 4 cut(s) 422, 655, 891, 928
MaeI CTAG 1 cut(s) 351
MaeII ACGT 2 cut(s) 377, 1002
MaeIII GTNAC 5 cut(s) 155, 346, 446, 479, 956
MalI GATC 5 cut(s) 136, 525, 576, 686, 690
MboI GATC 5 cut(s) 134, 523, 574, 684, 688
MboII GAAGA 5 cut(s) 88, 132, 144, 253, 899
MflI RGATCY 3 cut(s) 134, 523, 574
MhlI GDGCHC 3 cut(s) 444, 723, 912
MluCI AATT 5 cut(s) 399, 491, 546, 869, 881
MlyI GAGTC 1 cut(s) 717
MmeI TCCRAC 1 cut(s) 560
MnlI CCTC 6 cut(s) 295, 337, 384, 706, 1024, 1027
MroXI GAANNNNTTC 1 cut(s) 27
MseI TTAA 4 cut(s) 147, 339, 813, 972
MslI CAYNNNNRTG 2 cut(s) 660, 1030
MspA1I CMGCKG 2 cut(s) 153, 292
MspR9I CCNGG 1 cut(s) 802
Mva1269I GAATGC 1 cut(s) 560
MvaI CCWGG 1 cut(s) 802
MvnI CGCG 2 cut(s) 10, 153
MwoI GCNNNNNNNGC 5 cut(s) 32, 170, 901, 994, 1003
NdeI CATATG 1 cut(s) 897
NdeII GATC 5 cut(s) 134, 523, 574, 684, 688
NlaIII CATG 4 cut(s) 91, 739, 811, 1035
NlaIV GGNNCC 2 cut(s) 525, 576
NmuCI GTSAC 3 cut(s) 446, 479, 956
NspI RCATGY 1 cut(s) 811
PaeR7I CTCGAG 1 cut(s) 423
PctI GAATGC 1 cut(s) 560
PdmI GAANNNNTTC 1 cut(s) 27
PfeI GAWTC 4 cut(s) 5, 193, 581, 652
PkrI GCNGC 8 cut(s) 14, 175, 294, 678, 983, 990, 993, 996
PleI GAGTC 1 cut(s) 716
PmaCI CACGTG 1 cut(s) 1003
PmlI CACGTG 1 cut(s) 1003
PpsI GAGTC 1 cut(s) 716
Ppu21I YACGTR 1 cut(s) 1003
PsiI TTATAA 2 cut(s) 506, 545
Psp6I CCWGG 1 cut(s) 800
PspCI CACGTG 1 cut(s) 1003
PspGI CCWGG 1 cut(s) 800
PspN4I GGNNCC 2 cut(s) 525, 576
PspPI GGNCC 1 cut(s) 792
PstI CTGCAG 1 cut(s) 297
PsuI RGATCY 3 cut(s) 134, 523, 574
PvuII CAGCTG 1 cut(s) 292
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
RseI CAYNNNNRTG 2 cut(s) 660, 1030
SacII CCGCGG 1 cut(s) 154
SaqAI TTAA 4 cut(s) 147, 339, 813, 972
SatI GCNGC 8 cut(s) 13, 174, 293, 677, 982, 989, 992, 995
Sau3AI GATC 5 cut(s) 134, 523, 574, 684, 688
Sau96I GGNCC 1 cut(s) 792
SchI GAGTC 1 cut(s) 717
ScrFI CCNGG 1 cut(s) 802
SduI GDGCHC 3 cut(s) 444, 723, 912
SfaNI GCATC 4 cut(s) 422, 655, 891, 928
SfcI CTRYAG 1 cut(s) 293
Sfr274I CTCGAG 1 cut(s) 423
Sfr303I CCGCGG 1 cut(s) 154
SgrBI CCGCGG 1 cut(s) 154
SlaI CTCGAG 1 cut(s) 423
SmiMI CAYNNNNRTG 2 cut(s) 660, 1030
SmlI CTYRAG 2 cut(s) 423, 840
SmoI CTYRAG 2 cut(s) 423, 840
SpeI ACTAGT 1 cut(s) 350
Sse9I AATT 5 cut(s) 399, 491, 546, 869, 881
SsiI CCGC 7 cut(s) 151, 153, 181, 221, 979, 982, 992
SspMI CTAG 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 800
TaaI ACNGT 2 cut(s) 66, 824
TaiI ACGT 2 cut(s) 380, 1005
TaqI TCGA 3 cut(s) 424, 687, 711
TasI AATT 5 cut(s) 399, 491, 546, 869, 881
TauI GCSGC 2 cut(s) 984, 994
TfiI GAWTC 4 cut(s) 5, 193, 581, 652
Tru1I TTAA 4 cut(s) 147, 339, 813, 972
Tru9I TTAA 4 cut(s) 147, 339, 813, 972
TscAI CASTG 3 cut(s) 451, 829, 948
TseFI GTSAC 3 cut(s) 446, 479, 956
TseI GCWGC 6 cut(s) 12, 173, 292, 676, 988, 994
Tsp45I GTSAC 3 cut(s) 446, 479, 956
TspDTI ATGAA 3 cut(s) 104, 644, 894
TspRI CASTG 3 cut(s) 451, 829, 948
VneI GTGCAC 1 cut(s) 719
XapI RAATTY 1 cut(s) 881
XceI RCATGY 1 cut(s) 811
XhoI CTCGAG 1 cut(s) 423
XmiI GTMKAC 1 cut(s) 819
XmnI GAANNNNTTC 1 cut(s) 27
XspI CTAG 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.