pycom07g16940

Tryptophan/tyrosine permease family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013714
Physical Location & Seq
Reverse (-)
19443285 .. 19445576
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1548 bp
ATGGAATCAAAACTCTTGTTTTCATCGACATCAACTACCTCCTTCCATCTCAAAAGAAGAGTTCACCAAAACAGCCCAAGTATTTGCATAAAACTCAACACCACTGTTCCTCTCAGAAGTAGCAGCTCCAGCTACAATGCTTGTTCCTGCCACCAGCTGCTGCTCCAGCAGCAGCAGAAAAAGCACTCATCATTTACTCCGAAGGCTTCTGCAAAGAATGCACAGACAGGTCCTACACAGTTGGCACTAAACCCTACCAAGGAGCCCAAGAAGAAAGGAACCATCGCTGGCGCAGTTGCTTTGATCATCGGTACTAGTATAGGCTCGGGATCGATCCTTGCACTCCCGGAGAAAGCTTCTCCAGCAGGATTGCTTCCAAGTTCAATATCCATGGTGGTATGTTGGGGGTTTCTCCTGATCGAGGCGATTTTGCTTGTCGAGATCAATGTAAGTATGAGGAGGAAGAAGCGAATCGAAGACAATGCTGAAAATGAACTGGAGGTGATTTCTATTAGGACTATGGCCCAAGAGACTCTAGGAGACTTTGGTGGAACTCTAGTCACTCTGATTTATATTTTCTTAGGCTACACTTCAGTCATTGCTTATAGTTCCAAGTCTGGAGATATTCTCTTCCATTTGATCAATCTTCCGGCTCCAGTTTCCGGCTTCTTTTTTACCATGTTTTTTACCCTGCTCATCGCCATAGGTGGGACTCGAGCCACTGATCAAGTTAACCAACTCCTTACTGCTTCCATGATAGGTTTATTACTAGCAATAGAGGTGCTAGCAGTTGTGTTTGGAGGGTGGTCAGGAGTAGAGGGAAGTGGAGATTGGGGGAAGGTCCCCGCTACGGTACCTGTGATTATCTTTGCTCTGGTGTATCATGACCTAGCACCCGTTCTTTGTGCTTATTTGGGAAGTGATCTCGCACGCCTAAGGATTTCGGTATTTCTTGGTAGTCTTGTTCCATTGCTAGCATTGCTCGTTTGGGATGCAATTGCACTTGGCCTCTCTGCGCAGGCCGATCAAGTTGTTGACCCTGTTGAGTTGCTTATGAGGGTGAAATGGAGTGGGATTTCATATATGGTAGAAGCTTTCTCACTCCTTGCAATTGGAACATCATTAATTGGTACTCTTCTTGGCTTCTCTGAGTTTTTCAAAGAACAACTTAACAACCTCTCGTCGATATCTTCTCCCACGCAAACACTCGAGAAACCGGACATCACATTCGAGCTGAGAAAATGGTGGGGAAGAAATCAAATTAGCCTTGCAGCAGTGGCAATGGTTGTTGGTCCATCTCTTATTGTGTCGACTAGTATTCCAGATGCATTCTCTACGGCCACAGACATTGCTGGAGGGTATGGTATGACAATGCTATATGGAGTTCTACCGCCGGCAATGGCGTGGGCGATGCACAACAAAGAATGTGAAAATGAGGAGCAAAAAACTTTATCAAGAGCAAGGCCTGTACTAGTTGGGGCAGGACTCTTTGCTTGTGCAATAGTGGGAGAGCAAATCGTACAGGATATCCTCGCATTTCATTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trp_Tyr_perm

Protein Analysis

516

Amino Acids

55.3

Weight (kDa)

7.08

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trp_Tyr_perm PF03222 94 - 476 5.7e-42 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013714)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g19650 FvH4_7g19650
malus_domestica MD07G1175000.v1.1
prunus_persica Prupe.2G214200_v2.0.a1 Prupe.2G214200_v2.0.a1 Prupe.2G214200_v2.0.a1
pyrus_communis pycom07g16940
rosa_chinensis RchiOBHm_Chr1g0363461
rosa_laevigata RLG00000027564
rosa_multiflora Rmu_sc0004458.1_g000002
rosa_roxburghii Rroxscaffold_4G00293160
rosa_rugosa Rorug01G0306900 Rorug01G0307000
rosa_samantha Rh1AG316100 Rh1BG279400 Rh1CG295700 Rh1DG310500
rosa_wichuraiana Rw1G027990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1017
Acc65I GGTACC 1 cut(s) 853
AccB1I GGYRCC 1 cut(s) 853
AccI GTMKAC 1 cut(s) 1310
AciI CCGC 2 cut(s) 846, 1391
AclWI GGATC 2 cut(s) 328, 337
AcoI YGGCCR 1 cut(s) 1338
AcuI CTGAAG 1 cut(s) 576
AfaI GTAC 5 cut(s) 313, 855, 1132, 1470, 1521
AfiI CCNNNNNNNGG 5 cut(s) 259, 421, 662, 708, 850
AgsI TTSAA 2 cut(s) 384, 1159
AhlI ACTAGT 3 cut(s) 314, 1313, 1471
AjuI GAANNNNNNNTTGG 2 cut(s) 814, 846
AluBI AGCT 6 cut(s) 126, 132, 157, 356, 1094, 1234
AluI AGCT 6 cut(s) 126, 132, 157, 356, 1094, 1234
Alw26I GTCTC 2 cut(s) 524, 534
AlwI GGATC 2 cut(s) 328, 337
AlwNI CAGNNNCTG 1 cut(s) 160
Ama87I CYCGRG 3 cut(s) 325, 714, 1208
AoxI GGCC 5 cut(s) 522, 1006, 1020, 1338, 1463
ApeKI GCWGC 6 cut(s) 123, 157, 160, 169, 172, 1271
ArsI GACNNNNNNTTYG 2 cut(s) 1211, 1243
AseI ATTAAT 1 cut(s) 1124
Asp718I GGTACC 1 cut(s) 853
AspLEI GCGC 2 cut(s) 293, 1018
AspS9I GGNCC 4 cut(s) 230, 523, 841, 1292
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 3 cut(s) 56, 514, 1072
AsuNHI GCTAGC 2 cut(s) 784, 973
AvaI CYCGRG 3 cut(s) 325, 714, 1208
AvaII GGWCC 3 cut(s) 230, 841, 1292
AxyI CCTNAGG 1 cut(s) 935
BanI GGYRCC 1 cut(s) 853
BanII GRGCYC 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 483
BbvI GCAGC 6 cut(s) 135, 144, 147, 181, 184, 1283
BccI CCATC 3 cut(s) 54, 290, 1303
BceAI ACGGC 1 cut(s) 1353
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BclI TGATCA 3 cut(s) 303, 639, 724
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 2 cut(s) 524, 534
BcuI ACTAGT 3 cut(s) 314, 1313, 1471
BisI GCNGC 6 cut(s) 124, 158, 161, 170, 173, 1272
BlsI GCNGC 6 cut(s) 125, 159, 162, 171, 174, 1273
Bme1390I CCNGG 1 cut(s) 347
Bme18I GGWCC 3 cut(s) 230, 841, 1292
BmeT110I CYCGRG 3 cut(s) 325, 714, 1208
BmgT120I GGNCC 4 cut(s) 230, 523, 841, 1292
BmiI GGNNCC 5 cut(s) 264, 280, 654, 843, 855
BmrFI CCNGG 1 cut(s) 347
BmsI GCATC 3 cut(s) 982, 1315, 1401
BmtI GCTAGC 2 cut(s) 788, 977
BpiI GAAGAC 1 cut(s) 483
BplI GAGNNNNNCTC 2 cut(s) 612, 644
BpmI CTGGAG 7 cut(s) 112, 149, 345, 518, 639, 639, 1374
BpuMI CCSGG 1 cut(s) 347
Bsa29I ATCGAT 1 cut(s) 332
BsaJI CCNNGG 2 cut(s) 258, 390
BsaWI WCCGGW 1 cut(s) 1216
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 421, 662, 708, 850
Bse118I RCCGGY 1 cut(s) 1393
Bse1I ACTGG 2 cut(s) 501, 656
Bse21I CCTNAGG 1 cut(s) 935
Bse3DI GCAATG 6 cut(s) 597, 968, 977, 1287, 1347, 1404
BseCI ATCGAT 1 cut(s) 332
BseDI CCNNGG 2 cut(s) 258, 390
BseGI GGATG 1 cut(s) 997
BseLI CCNNNNNNNGG 5 cut(s) 259, 421, 662, 708, 850
BseMI GCAATG 6 cut(s) 597, 968, 977, 1287, 1347, 1404
BseMII CTCAG 3 cut(s) 127, 1140, 1226
BseNI ACTGG 2 cut(s) 501, 656
BseRI GAGGAG 2 cut(s) 472, 1451
BseXI GCAGC 6 cut(s) 135, 144, 147, 181, 184, 1283
BshFI GGCC 5 cut(s) 524, 1008, 1022, 1340, 1465
BshNI GGYRCC 1 cut(s) 853
BshVI ATCGAT 1 cut(s) 332
BsiHKCI CYCGRG 3 cut(s) 325, 714, 1208
BsiSI CCGG 5 cut(s) 347, 650, 663, 1217, 1394
BslFI GGGAC 2 cut(s) 724, 827
BslI CCNNNNNNNGG 5 cut(s) 259, 421, 662, 708, 850
BsmAI GTCTC 2 cut(s) 524, 534
BsmFI GGGAC 2 cut(s) 724, 827
BsmI GAATGC 2 cut(s) 223, 1328
BsnI GGCC 5 cut(s) 524, 1008, 1022, 1340, 1465
BsoBI CYCGRG 3 cut(s) 325, 714, 1208
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 9 cut(s) 303, 329, 333, 417, 441, 639, 724, 922, 1024
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 2 cut(s) 846, 1391
BspANI GGCC 5 cut(s) 524, 1008, 1022, 1340, 1465
BspCNI CTCAG 3 cut(s) 126, 1141, 1227
BspDI ATCGAT 1 cut(s) 332
BspHI TCATGA 1 cut(s) 883
BspLI GGNNCC 5 cut(s) 264, 280, 654, 843, 855
BspOI GCTAGC 2 cut(s) 788, 977
BspPI GGATC 2 cut(s) 328, 337
BspT107I GGYRCC 1 cut(s) 853
BsrDI GCAATG 6 cut(s) 597, 968, 977, 1287, 1347, 1404
BsrFI RCCGGY 1 cut(s) 1393
BsrI ACTGG 2 cut(s) 501, 656
BssAI RCCGGY 1 cut(s) 1393
BssECI CCNNGG 2 cut(s) 258, 390
BssMI GATC 9 cut(s) 303, 329, 333, 417, 441, 639, 724, 922, 1024
BssT1I CCWWGG 2 cut(s) 258, 390
Bst4CI ACNGT 3 cut(s) 106, 240, 853
Bst6I CTCTTC 3 cut(s) 52, 635, 1140
BstAPI GCANNNNNTGC 1 cut(s) 218
BstC8I GCNNGC 6 cut(s) 289, 786, 931, 975, 1020, 1395
BstDEI CTNAG 5 cut(s) 113, 580, 935, 1149, 1235
BstDSI CCRYGG 1 cut(s) 390
BstENI CCTNNNNNAGG 1 cut(s) 419
BstF5I GGATG 1 cut(s) 997
BstHHI GCGC 2 cut(s) 293, 1018
BstKTI GATC 9 cut(s) 306, 332, 336, 420, 444, 642, 727, 925, 1027
BstMAI GTCTC 2 cut(s) 524, 534
BstMBI GATC 9 cut(s) 303, 329, 333, 417, 441, 639, 724, 922, 1024
BstMWI GCNNNNNNNGC 8 cut(s) 129, 166, 169, 181, 218, 362, 979, 1277
BstSCI CCNGG 1 cut(s) 345
BstV1I GCAGC 6 cut(s) 135, 144, 147, 181, 184, 1283
BstV2I GAAGAC 1 cut(s) 483
Bsu15I ATCGAT 1 cut(s) 332
Bsu36I CCTNAGG 1 cut(s) 935
BsuRI GGCC 5 cut(s) 524, 1008, 1022, 1340, 1465
BsuTUI ATCGAT 1 cut(s) 332
BtgI CCRYGG 1 cut(s) 390
BtgZI GCGATG 3 cut(s) 268, 682, 1424
BtsCI GGATG 1 cut(s) 997
BtsI GCAGTG 1 cut(s) 1281
BtsIMutI CAGTG 3 cut(s) 102, 720, 1281
Cac8I GCNNGC 6 cut(s) 289, 786, 931, 975, 1020, 1395
CaiI CAGNNNCTG 1 cut(s) 160
CciI TCATGA 1 cut(s) 883
CfoI GCGC 2 cut(s) 293, 1018
Cfr10I RCCGGY 1 cut(s) 1393
Cfr13I GGNCC 4 cut(s) 230, 523, 841, 1292
ClaI ATCGAT 1 cut(s) 332
Csp6I GTAC 5 cut(s) 312, 854, 1131, 1469, 1520
CspCI CAANNNNNGTGG 2 cut(s) 1330, 1365
CviAII CATG 4 cut(s) 391, 679, 754, 884
CviQI GTAC 5 cut(s) 312, 854, 1131, 1469, 1520
DdeI CTNAG 5 cut(s) 113, 580, 935, 1149, 1235
DpnI GATC 9 cut(s) 305, 331, 335, 419, 443, 641, 726, 924, 1026
DpnII GATC 9 cut(s) 303, 329, 333, 417, 441, 639, 724, 922, 1024
EaeI YGGCCR 1 cut(s) 1338
Eam1104I CTCTTC 3 cut(s) 52, 635, 1140
EarI CTCTTC 3 cut(s) 52, 635, 1140
Eco130I CCWWGG 2 cut(s) 258, 390
Eco147I AGGCCT 1 cut(s) 1465
Eco24I GRGCYC 1 cut(s) 267
Eco32I GATATC 2 cut(s) 1188, 1528
Eco47I GGWCC 3 cut(s) 230, 841, 1292
Eco57I CTGAAG 1 cut(s) 576
Eco81I CCTNAGG 1 cut(s) 935
Eco88I CYCGRG 3 cut(s) 325, 714, 1208
EcoNI CCTNNNNNAGG 1 cut(s) 419
EcoO109I RGGNCCY 2 cut(s) 230, 841
EcoRV GATATC 2 cut(s) 1188, 1528
EcoT14I CCWWGG 2 cut(s) 258, 390
EcoT22I ATGCAT 1 cut(s) 1330
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 258, 390
FaeI CATG 4 cut(s) 394, 682, 757, 887
FalI AAGNNNNNCTT 2 cut(s) 1152, 1184
FaqI GGGAC 2 cut(s) 724, 827
FatI CATG 4 cut(s) 390, 678, 753, 883
FauI CCCGC 1 cut(s) 853
FbaI TGATCA 3 cut(s) 303, 639, 724
FblI GTMKAC 1 cut(s) 1310
Fnu4HI GCNGC 6 cut(s) 124, 158, 161, 170, 173, 1272
FokI GGATG 1 cut(s) 1004
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 6 cut(s) 124, 158, 161, 170, 173, 1272
FspI TGCGCA 1 cut(s) 1017
GlaI GCGC 2 cut(s) 292, 1017
GluI GCNGC 6 cut(s) 124, 158, 161, 170, 173, 1272
GsuI CTGGAG 7 cut(s) 112, 149, 345, 518, 639, 639, 1374
HaeIII GGCC 5 cut(s) 524, 1008, 1022, 1340, 1465
HapII CCGG 5 cut(s) 347, 650, 663, 1217, 1394
HhaI GCGC 2 cut(s) 293, 1018
Hin1II CATG 4 cut(s) 394, 682, 757, 887
Hin6I GCGC 2 cut(s) 291, 1016
HinP1I GCGC 2 cut(s) 291, 1016
HincII GTYRAC 3 cut(s) 733, 1036, 1311
HindII GTYRAC 3 cut(s) 733, 1036, 1311
HindIII AAGCTT 2 cut(s) 354, 1092
HinfI GANTC 5 cut(s) 5, 471, 532, 712, 1485
HpaI GTTAAC 1 cut(s) 733
HpaII CCGG 5 cut(s) 347, 650, 663, 1217, 1394
HphI GGTGA 3 cut(s) 56, 514, 1072
Hpy166II GTNNAC 4 cut(s) 64, 733, 1036, 1311
Hpy188I TCNGA 4 cut(s) 116, 201, 567, 1150
Hpy188III TCNNGA 9 cut(s) 327, 415, 439, 618, 810, 884, 1210, 1322, 1455
Hpy8I GTNNAC 4 cut(s) 64, 733, 1036, 1311
Hpy99I CGWCG 1 cut(s) 1186
HpyAV CCTTC 3 cut(s) 52, 196, 832
HpyCH4III ACNGT 3 cut(s) 106, 240, 853
HpyF10VI GCNNNNNNNGC 8 cut(s) 129, 166, 169, 181, 218, 362, 979, 1277
HpyF3I CTNAG 5 cut(s) 113, 580, 935, 1149, 1235
Hsp92II CATG 4 cut(s) 394, 682, 757, 887
HspAI GCGC 2 cut(s) 291, 1016
KpnI GGTACC 1 cut(s) 857
KroI GCCGGC 1 cut(s) 1393
KroNI GCCGGC 1 cut(s) 1395
Ksp22I TGATCA 3 cut(s) 303, 639, 724
KspAI GTTAAC 1 cut(s) 733
Kzo9I GATC 9 cut(s) 303, 329, 333, 417, 441, 639, 724, 922, 1024
LmnI GCTCC 5 cut(s) 131, 168, 262, 658, 1438
Lsp1109I GCAGC 6 cut(s) 135, 144, 147, 181, 184, 1283
LweI GCATC 3 cut(s) 982, 1315, 1401
MaeIII GTNAC 1 cut(s) 559
MalI GATC 9 cut(s) 305, 331, 335, 419, 443, 641, 726, 924, 1026
MboI GATC 9 cut(s) 303, 329, 333, 417, 441, 639, 724, 922, 1024
MboII GAAGA 9 cut(s) 69, 283, 475, 488, 622, 638, 1127, 1182, 1263
MfeI CAATTG 2 cut(s) 996, 1110
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 4 cut(s) 996, 1110, 1125, 1260
MlyI GAGTC 3 cut(s) 526, 706, 1479
Mph1103I ATGCAT 1 cut(s) 1330
MroNI GCCGGC 1 cut(s) 1393
MseI TTAA 3 cut(s) 732, 1124, 1170
MspA1I CMGCKG 1 cut(s) 157
MspI CCGG 5 cut(s) 347, 650, 663, 1217, 1394
MspR9I CCNGG 1 cut(s) 347
MunI CAATTG 2 cut(s) 996, 1110
Mva1269I GAATGC 2 cut(s) 223, 1328
MwoI GCNNNNNNNGC 8 cut(s) 129, 166, 169, 181, 218, 362, 979, 1277
NaeI GCCGGC 1 cut(s) 1395
NciI CCSGG 1 cut(s) 347
NcoI CCATGG 1 cut(s) 390
NdeII GATC 9 cut(s) 303, 329, 333, 417, 441, 639, 724, 922, 1024
NgoMIV GCCGGC 1 cut(s) 1393
NheI GCTAGC 2 cut(s) 784, 973
NlaIII CATG 4 cut(s) 394, 682, 757, 887
NlaIV GGNNCC 5 cut(s) 264, 280, 654, 843, 855
NmuCI GTSAC 1 cut(s) 559
NsbI TGCGCA 1 cut(s) 1017
NsiI ATGCAT 1 cut(s) 1330
PaeR7I CTCGAG 2 cut(s) 714, 1208
PagI TCATGA 1 cut(s) 883
PceI AGGCCT 1 cut(s) 1465
PctI GAATGC 2 cut(s) 223, 1328
PdiI GCCGGC 1 cut(s) 1395
PfeI GAWTC 2 cut(s) 5, 471
PfoI TCCNGGA 1 cut(s) 345
PkrI GCNGC 6 cut(s) 125, 159, 162, 171, 174, 1273
PleI GAGTC 3 cut(s) 526, 706, 1479
PpsI GAGTC 3 cut(s) 526, 706, 1479
PpuMI RGGWCCY 2 cut(s) 230, 841
PshBI ATTAAT 1 cut(s) 1124
Psp5II RGGWCCY 2 cut(s) 230, 841
PspN4I GGNNCC 5 cut(s) 264, 280, 654, 843, 855
PspPI GGNCC 4 cut(s) 230, 523, 841, 1292
PspPPI RGGWCCY 2 cut(s) 230, 841
PspXI VCTCGAGB 1 cut(s) 714
PstNI CAGNNNCTG 1 cut(s) 160
PvuII CAGCTG 1 cut(s) 157
RsaI GTAC 5 cut(s) 313, 855, 1132, 1470, 1521
RsaNI GTAC 5 cut(s) 312, 854, 1131, 1469, 1520
SalI GTCGAC 1 cut(s) 1309
SaqAI TTAA 3 cut(s) 732, 1124, 1170
SatI GCNGC 6 cut(s) 124, 158, 161, 170, 173, 1272
Sau3AI GATC 9 cut(s) 303, 329, 333, 417, 441, 639, 724, 922, 1024
Sau96I GGNCC 4 cut(s) 230, 523, 841, 1292
SchI GAGTC 3 cut(s) 526, 706, 1479
ScrFI CCNGG 1 cut(s) 347
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 3 cut(s) 982, 1315, 1401
Sfr274I CTCGAG 2 cut(s) 714, 1208
SinI GGWCC 3 cut(s) 230, 841, 1292
SlaI CTCGAG 2 cut(s) 714, 1208
SmlI CTYRAG 2 cut(s) 714, 1208
SmoI CTYRAG 2 cut(s) 714, 1208
SpeI ACTAGT 3 cut(s) 314, 1313, 1471
Sse9I AATT 4 cut(s) 996, 1110, 1125, 1260
SseBI AGGCCT 1 cut(s) 1465
SsiI CCGC 2 cut(s) 846, 1391
StuI AGGCCT 1 cut(s) 1465
StyD4I CCNGG 1 cut(s) 345
StyI CCWWGG 2 cut(s) 258, 390
TaaI ACNGT 3 cut(s) 106, 240, 853
TasI AATT 4 cut(s) 996, 1110, 1125, 1260
TatI WGTACW 1 cut(s) 1468
TfiI GAWTC 2 cut(s) 5, 471
Tru1I TTAA 3 cut(s) 732, 1124, 1170
Tru9I TTAA 3 cut(s) 732, 1124, 1170
TscAI CASTG 3 cut(s) 109, 727, 1281
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 6 cut(s) 123, 157, 160, 169, 172, 1271
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 4 cut(s) 12, 507, 1068, 1529
TspRI CASTG 3 cut(s) 109, 727, 1281
VpaK11BI GGWCC 3 cut(s) 230, 841, 1292
VspI ATTAAT 1 cut(s) 1124
XagI CCTNNNNNAGG 1 cut(s) 419
XhoI CTCGAG 2 cut(s) 714, 1208
XmiI GTMKAC 1 cut(s) 1310
Zsp2I ATGCAT 1 cut(s) 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.