pycom07g22300

Kinase-like protein TMKL1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013548
Physical Location & Seq
Forward (+)
23681394 .. 23682754
1361 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g22300.2

Sequence Viewer

Length: 783 bp
ATGAGGCCTGTTTGCACTGCAAAAGTTGAAGATTTTGGTGAAGTTGTTCGGCATTTGGGGTGCGTAAGGCATCATAATTTGGTGCCTCTTTTGGGATTTTATGCAGGGCCAAGAGGTGAGAAGCTTCTGATTCATCCGTTTTATTGGCGCGGCAATCTGGCTCAATTTGTGAGAGAAAGCAACCCTGACTCTCACAGATGGCCCATAATCTATAGGATCTCAATTGGTATAGCCAAAGGCTTGGATCACCTTCACACTGGCTTCGAAAAGCCAATAATTCACGGCAATCTCAAGTCGAAAAACATACTCTTGGACCGCCATTGTCGCCCTTTCATCTCGGATTTCAGCTTGCATCTTCTGTTGAATCCAACTGCTGGCCAGGAAATGCTTGAAGTCTCAGCATCCGAAGGCTACAAAGCACCTGAACTGATAAAAATGCGGGATGCGAATGAAGAGACTGATATATACAGTCTTGGGGTTGTACTGCTTGAATTGCTCACAGGGAAGGAACCAATTAATCAAAATCCAACTACACCCGACGAGGATTTTAGTTTGCCAAATTTCATGAGAAATGCAGTGCTTGGACATAAAATCCATGACTTGTTCCATCCAGATTTGCTTCTCAATAGCGGCGTCGGCATCGTCGACGGTGAAATCCCGGTCACTAGAGAACAGATTCTCAAATTCTTTCAGCTTGCTATGGCATGTTGTGCTCCTTCTCCAACGCTTAGACCAAACACCAAGAAAGTTCTGTGGAAGCTTGAAGATATCGGAAGAAACTGA

Protein Analysis

261

Amino Acids

29.39

Weight (kDa)

6.89

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07620
fragaria_vesca FvH4_7g27020
malus_domestica MD01G1177800.v1.1 MD07G1249600.v1.1
prunus_persica Prupe.2G273300_v2.0.a1
pyrus_communis pycom01g18860 pycom07g22290 pycom07g22300
rosa_chinensis RchiOBHm_Chr1g0373821
rosa_laevigata RLG00000026803
rosa_multiflora Rmu_sc0013735.1_g000009
rosa_roxburghii Rroxscaffold_4G00283980
rosa_rugosa Rorug01G0379500
rosa_samantha Rh1AG389000 Rh1BG353200 Rh1CG366400 Rh1DG384100
rosa_wichuraiana Rw1G034620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 374
AccI GTMKAC 1 cut(s) 645
AccII CGCG 1 cut(s) 150
AciI CCGC 4 cut(s) 150, 316, 439, 630
AclWI GGATC 2 cut(s) 224, 252
AcoI YGGCCR 1 cut(s) 376
AcsI RAATTY 2 cut(s) 559, 683
AcyI GRCGYC 1 cut(s) 633
AfaI GTAC 1 cut(s) 483
AfiI CCNNNNNNNGG 2 cut(s) 92, 374
AgsI TTSAA 5 cut(s) 29, 364, 392, 491, 764
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 4 cut(s) 124, 348, 694, 760
AluI AGCT 4 cut(s) 124, 348, 694, 760
Alw21I GWGCWC 1 cut(s) 715
Alw26I GTCTC 2 cut(s) 400, 449
AlwI GGATC 2 cut(s) 224, 252
AoxI GGCC 4 cut(s) 5, 107, 200, 376
ApoI RAATTY 2 cut(s) 559, 683
AseI ATTAAT 1 cut(s) 516
Asp700I GAANNNNTTC 2 cut(s) 45, 675
AspLEI GCGC 1 cut(s) 150
AspS9I GGNCC 3 cut(s) 107, 201, 313
AsuC2I CCSGG 1 cut(s) 659
AsuHPI GGTGA 4 cut(s) 50, 128, 239, 662
AsuII TTCGAA 1 cut(s) 264
AvaII GGWCC 1 cut(s) 313
BalI TGGCCA 1 cut(s) 378
BanI GGYRCC 1 cut(s) 82
Bbv12I GWGCWC 1 cut(s) 715
BccI CCATC 2 cut(s) 192, 615
BceAI ACGGC 1 cut(s) 298
BciT130I CCWGG 1 cut(s) 380
BcnI CCSGG 1 cut(s) 659
BcoDI GTCTC 2 cut(s) 400, 449
BfaI CTAG 1 cut(s) 666
BfmI CTRYAG 1 cut(s) 211
BisI GCNGC 2 cut(s) 151, 631
BlsI GCNGC 2 cut(s) 152, 632
Bme1390I CCNGG 2 cut(s) 380, 659
Bme18I GGWCC 1 cut(s) 313
BmgT120I GGNCC 3 cut(s) 107, 201, 313
BmiI GGNNCC 2 cut(s) 84, 510
BmrFI CCNGG 2 cut(s) 380, 659
BmsI GCATC 5 cut(s) 79, 361, 410, 433, 648
Bpu14I TTCGAA 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 659
BsaHI GRCGYC 1 cut(s) 633
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 374
Bse1I ACTGG 1 cut(s) 262
BseBI CCWGG 1 cut(s) 380
BseGI GGATG 4 cut(s) 133, 401, 448, 607
BseLI CCNNNNNNNGG 2 cut(s) 92, 374
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 262
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 4 cut(s) 7, 109, 202, 378
BshNI GGYRCC 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 715
BsiSI CCGG 1 cut(s) 659
BslI CCNNNNNNNGG 2 cut(s) 92, 374
BsmAI GTCTC 2 cut(s) 400, 449
BsnI GGCC 4 cut(s) 7, 109, 202, 378
Bsp119I TTCGAA 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 715
Bsp143I GATC 2 cut(s) 216, 244
BspACI CCGC 4 cut(s) 150, 316, 439, 630
BspANI GGCC 4 cut(s) 7, 109, 202, 378
BspCNI CTCAG 1 cut(s) 410
BspFNI CGCG 1 cut(s) 150
BspHI TCATGA 1 cut(s) 564
BspLI GGNNCC 2 cut(s) 84, 510
BspPI GGATC 2 cut(s) 224, 252
BspT104I TTCGAA 1 cut(s) 264
BspT107I GGYRCC 1 cut(s) 82
BsrI ACTGG 1 cut(s) 262
BssMI GATC 2 cut(s) 216, 244
BssNI GRCGYC 1 cut(s) 633
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 2 cut(s) 470, 650
Bst6I CTCTTC 1 cut(s) 447
BstACI GRCGYC 1 cut(s) 633
BstAPI GCANNNNNTGC 1 cut(s) 710
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 3 cut(s) 350, 376, 696
BstDEI CTNAG 2 cut(s) 397, 728
BstF5I GGATG 4 cut(s) 133, 401, 448, 607
BstFNI CGCG 1 cut(s) 150
BstHHI GCGC 1 cut(s) 150
BstKTI GATC 2 cut(s) 219, 247
BstMAI GTCTC 2 cut(s) 400, 449
BstMBI GATC 2 cut(s) 216, 244
BstMWI GCNNNNNNNGC 4 cut(s) 324, 493, 636, 710
BstNI CCWGG 1 cut(s) 380
BstNSI RCATGY 1 cut(s) 708
BstSCI CCNGG 2 cut(s) 378, 657
BstSFI CTRYAG 1 cut(s) 211
BstUI CGCG 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 216
BstXI CCANNNNNNTGG 1 cut(s) 241
BstYI RGATCY 1 cut(s) 216
BsuRI GGCC 4 cut(s) 7, 109, 202, 378
BtsCI GGATG 4 cut(s) 133, 401, 448, 607
BtsI GCAGTG 2 cut(s) 15, 582
BtsIMutI CAGTG 3 cut(s) 15, 255, 582
Cac8I GCNNGC 3 cut(s) 350, 376, 696
CciI TCATGA 1 cut(s) 564
CfoI GCGC 1 cut(s) 150
Cfr13I GGNCC 3 cut(s) 107, 201, 313
CseI GACGC 1 cut(s) 622
Csp6I GTAC 1 cut(s) 482
CviAII CATG 3 cut(s) 565, 596, 705
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 2 cut(s) 397, 728
DpnI GATC 2 cut(s) 218, 246
DpnII GATC 2 cut(s) 216, 244
EaeI YGGCCR 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
Eco147I AGGCCT 1 cut(s) 7
Eco32I GATATC 1 cut(s) 769
Eco47I GGWCC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 378
EcoRV GATATC 1 cut(s) 769
FaeI CATG 3 cut(s) 568, 599, 708
FatI CATG 3 cut(s) 564, 595, 704
FauI CCCGC 1 cut(s) 432
FblI GTMKAC 1 cut(s) 645
Fnu4HI GCNGC 2 cut(s) 151, 631
FokI GGATG 4 cut(s) 120, 388, 455, 594
Fsp4HI GCNGC 2 cut(s) 151, 631
FspBI CTAG 1 cut(s) 666
GlaI GCGC 1 cut(s) 149
GluI GCNGC 2 cut(s) 151, 631
HaeIII GGCC 4 cut(s) 7, 109, 202, 378
HapII CCGG 1 cut(s) 659
HgaI GACGC 1 cut(s) 622
HhaI GCGC 1 cut(s) 150
Hin1I GRCGYC 1 cut(s) 633
Hin1II CATG 3 cut(s) 568, 599, 708
Hin6I GCGC 1 cut(s) 148
HinP1I GCGC 1 cut(s) 148
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HindIII AAGCTT 2 cut(s) 122, 758
HinfI GANTC 4 cut(s) 130, 188, 364, 676
HpaII CCGG 1 cut(s) 659
HphI GGTGA 4 cut(s) 50, 128, 239, 662
Hpy166II GTNNAC 1 cut(s) 646
Hpy188I TCNGA 4 cut(s) 129, 340, 406, 773
Hpy188III TCNNGA 2 cut(s) 565, 611
Hpy8I GTNNAC 1 cut(s) 646
Hpy99I CGWCG 4 cut(s) 542, 638, 647, 650
HpyAV CCTTC 4 cut(s) 260, 401, 499, 726
HpyCH4III ACNGT 2 cut(s) 470, 650
HpyCH4V TGCA 5 cut(s) 15, 20, 104, 352, 575
HpyF10VI GCNNNNNNNGC 4 cut(s) 324, 493, 636, 710
HpyF3I CTNAG 2 cut(s) 397, 728
Hsp92I GRCGYC 1 cut(s) 633
Hsp92II CATG 3 cut(s) 568, 599, 708
HspAI GCGC 1 cut(s) 148
Kzo9I GATC 2 cut(s) 216, 244
LmnI GCTCC 1 cut(s) 718
LweI GCATC 5 cut(s) 79, 361, 410, 433, 648
MaeI CTAG 1 cut(s) 666
MaeIII GTNAC 1 cut(s) 661
MalI GATC 2 cut(s) 218, 246
MboI GATC 2 cut(s) 216, 244
MboII GAAGA 4 cut(s) 41, 347, 464, 776
MfeI CAATTG 1 cut(s) 222
MflI RGATCY 1 cut(s) 216
MhlI GDGCHC 1 cut(s) 715
MlsI TGGCCA 1 cut(s) 378
MluCI AATT 8 cut(s) 76, 164, 222, 276, 491, 513, 559, 683
MluNI TGGCCA 1 cut(s) 378
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 3 cut(s) 392, 551, 746
MnlI CCTC 3 cut(s) 96, 107, 535
Mox20I TGGCCA 1 cut(s) 378
MroXI GAANNNNTTC 2 cut(s) 45, 675
MscI TGGCCA 1 cut(s) 378
MseI TTAA 1 cut(s) 516
Msp20I TGGCCA 1 cut(s) 378
MspI CCGG 1 cut(s) 659
MspR9I CCNGG 2 cut(s) 380, 659
MunI CAATTG 1 cut(s) 222
MvaI CCWGG 1 cut(s) 380
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 4 cut(s) 324, 493, 636, 710
NciI CCSGG 1 cut(s) 659
NdeII GATC 2 cut(s) 216, 244
NlaIII CATG 3 cut(s) 568, 599, 708
NlaIV GGNNCC 2 cut(s) 84, 510
NmuCI GTSAC 1 cut(s) 661
NspI RCATGY 1 cut(s) 708
NspV TTCGAA 1 cut(s) 264
PagI TCATGA 1 cut(s) 564
PceI AGGCCT 1 cut(s) 7
PcsI WCGNNNNNNNCGW 1 cut(s) 642
PdmI GAANNNNTTC 2 cut(s) 45, 675
PfeI GAWTC 3 cut(s) 130, 364, 676
PflMI CCANNNNNTGG 1 cut(s) 374
PkrI GCNGC 2 cut(s) 152, 632
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
PshBI ATTAAT 1 cut(s) 516
Psp6I CCWGG 1 cut(s) 378
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 2 cut(s) 84, 510
PspPI GGNCC 3 cut(s) 107, 201, 313
PsuI RGATCY 1 cut(s) 216
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
SalI GTCGAC 1 cut(s) 644
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 2 cut(s) 151, 631
Sau3AI GATC 2 cut(s) 216, 244
Sau96I GGNCC 3 cut(s) 107, 201, 313
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 380, 659
SduI GDGCHC 1 cut(s) 715
SetI ASST 7 cut(s) 118, 126, 252, 350, 424, 696, 762
SfaNI GCATC 5 cut(s) 79, 361, 410, 433, 648
SfcI CTRYAG 1 cut(s) 211
SfuI TTCGAA 1 cut(s) 264
SgrDI CGTCGACG 1 cut(s) 644
SinI GGWCC 1 cut(s) 313
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 8 cut(s) 76, 164, 222, 276, 491, 513, 559, 683
SseBI AGGCCT 1 cut(s) 7
SsiI CCGC 4 cut(s) 150, 316, 439, 630
SspMI CTAG 1 cut(s) 666
StuI AGGCCT 1 cut(s) 7
StyD4I CCNGG 2 cut(s) 378, 657
TaaI ACNGT 2 cut(s) 470, 650
TaqI TCGA 3 cut(s) 264, 296, 645
TasI AATT 8 cut(s) 76, 164, 222, 276, 491, 513, 559, 683
TatI WGTACW 1 cut(s) 481
TauI GCSGC 2 cut(s) 153, 633
TfiI GAWTC 3 cut(s) 130, 364, 676
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 3 cut(s) 22, 262, 582
TseFI GTSAC 1 cut(s) 661
Tsp45I GTSAC 1 cut(s) 661
TspDTI ATGAA 4 cut(s) 122, 322, 465, 553
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 3 cut(s) 22, 262, 582
Van91I CCANNNNNTGG 1 cut(s) 374
VpaK11BI GGWCC 1 cut(s) 313
VspI ATTAAT 1 cut(s) 516
XapI RAATTY 2 cut(s) 559, 683
XceI RCATGY 1 cut(s) 708
XmiI GTMKAC 1 cut(s) 645
XmnI GAANNNNTTC 2 cut(s) 45, 675
XspI CTAG 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.