Rroxscaffold_4G00283980

Kinase-like protein TMKL1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013548
Physical Location & Seq
Reverse (-)
5742904 .. 5746102
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283980.1

Sequence Viewer

Length: 1092 bp
ATGAAGAACATCCACAAGCTCACTTTGATTCTGGTACTGACCTCAGCCACAGTTTTGGTATTTCTTGCATTTTTAATCTACTTTTGCCGCAAAAGTAGTCCAAAAACTGAGCTAAATGATGTAGAAAGCACAGAGCTGAAGCATGAAGATGATGGTGTTGTTCTTCAAGTGGAGGACTTGATAACTTTTCAGGATGGTGAGGACCTCACAATTTCTGACATACTGGAAGCTCCAGGAGAAGTGATTGGAAAATCCAACTATGGCACAGTTTACAAGGCTTTGTTGCAGACTAGTGACTCGGTGAAGTTGCTGAGGTTTTTGAGGCCTGTGTGTACTGCAAGAGTTGAAGATTTTGGTGAAGTGATTCAGCTTTTGGGCTGCATCAGGCACCCCAATTTGGTGCCTCTTCTGGGTTTCTATGCAGGGCCAAGAGGTGAGAAGCTTCTGATTCATCCGTATTATTGGCGTGGGAGTCTGGCTTCATTTGTGAGAGAGGGCAATCCCGAGTCTCACAAATGGGCGATAATTTATAGGATCTCAGTTGGTATAGCCAAAGGATTACATCATCTTCACACTGGCTTGGAGAAGCCAGTAATTCATGGTAATCTCAAGTTGAAAAATGTGCTCTTCGACCGGCATTACCAACCTTTCATCTCAGATTTCAGCCTACACCTTCTCTTGAATCCCACTGCCGCCCAAGAAATGCTTGAATCTTCGGCATTCCAAGGCTACAAGGCGCCCGAGTTGATCAAAATGAGGGATGCAAATGAAGAGACTGATATATACAGTTTCGGGATCATCTTACTTGAGTTACTCACAGGGAAGGAACCCATCAACCAAAACCCTACAACACCTGATGAGGACTTCAGCTTGCCGAACCTAATGAGAAATGCAGTCCTTGGACATAGAATTCATGAACTGTTCCATCCAGATATACTTGTCAGTAGCTGCACTACTGATGATGAAATCCCAGTTACTAAGGAAAGAATACTCAAATTGTTTCAGCTTGCCATGACATGTTGTTCTCCTTCACCATCACTGAGACCATACACCAAGCAAGTGCTATGGAAGCTCGAAGAGATTGGAAGCTAG

Protein Analysis

363

Amino Acids

40.99

Weight (kDa)

5.88

Isoelectric Point (pI)

40.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 80 - 355 2.4e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 80 - 355 3e-26 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07620
fragaria_vesca FvH4_7g27020
malus_domestica MD01G1177800.v1.1 MD07G1249600.v1.1
prunus_persica Prupe.2G273300_v2.0.a1
pyrus_communis pycom01g18860 pycom07g22290 pycom07g22300
rosa_chinensis RchiOBHm_Chr1g0373821
rosa_laevigata RLG00000026803
rosa_multiflora Rmu_sc0013735.1_g000009
rosa_roxburghii Rroxscaffold_4G00283980
rosa_rugosa Rorug01G0379500
rosa_samantha Rh1AG389000 Rh1BG353200 Rh1CG366400 Rh1DG384100
rosa_wichuraiana Rw1G034620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 387, 400, 736
AciI CCGC 2 cut(s) 88, 693
AclWI GGATC 2 cut(s) 542, 803
AcsI RAATTY 1 cut(s) 909
AcuI CTGAAG 2 cut(s) 158, 850
AcyI GRCGYC 1 cut(s) 737
AfaI GTAC 2 cut(s) 36, 334
AfiI CCNNNNNNNGG 2 cut(s) 397, 410
AflIII ACRYGT 1 cut(s) 1016
AgsI TTSAA 5 cut(s) 167, 347, 616, 682, 710
AhlI ACTAGT 1 cut(s) 290
AjnI CCWGG 1 cut(s) 232
Alw21I GWGCWC 1 cut(s) 627
Alw26I GTCTC 3 cut(s) 513, 767, 1036
AlwI GGATC 2 cut(s) 542, 803
AlwNI CAGNNNCTG 1 cut(s) 948
Ama87I CYCGRG 2 cut(s) 503, 740
AoxI GGCC 2 cut(s) 323, 425
ApeKI GCWGC 2 cut(s) 378, 948
ApoI RAATTY 1 cut(s) 909
Asp700I GAANNNNTTC 1 cut(s) 363
AspLEI GCGC 1 cut(s) 739
AspS9I GGNCC 2 cut(s) 202, 425
AsuHPI GGTGA 5 cut(s) 209, 313, 368, 446, 1023
AvaI CYCGRG 2 cut(s) 503, 740
AvaII GGWCC 1 cut(s) 202
BanI GGYRCC 3 cut(s) 387, 400, 736
Bbv12I GWGCWC 1 cut(s) 627
BbvCI CCTCAGC 2 cut(s) 43, 311
BbvI GCAGC 2 cut(s) 365, 935
BccI CCATC 5 cut(s) 146, 188, 839, 933, 1042
BciT130I CCWGG 1 cut(s) 234
BclI TGATCA 1 cut(s) 747
BcoDI GTCTC 3 cut(s) 513, 767, 1036
BcuI ACTAGT 1 cut(s) 290
BfaI CTAG 2 cut(s) 291, 1090
BfoI RGCGCY 1 cut(s) 740
BisI GCNGC 4 cut(s) 88, 379, 693, 949
BlsI GCNGC 4 cut(s) 89, 380, 694, 950
Bme1390I CCNGG 1 cut(s) 234
Bme18I GGWCC 1 cut(s) 202
BmeT110I CYCGRG 2 cut(s) 503, 740
BmgT120I GGNCC 2 cut(s) 202, 425
BmiI GGNNCC 4 cut(s) 389, 402, 738, 828
BmrFI CCNGG 1 cut(s) 234
BmrI ACTGGG 1 cut(s) 965
BmsI GCATC 2 cut(s) 390, 751
BmuI ACTGGG 1 cut(s) 965
BpmI CTGGAG 1 cut(s) 216
Bpu10I CCTNAGC 2 cut(s) 43, 311
BpuEI CTTGAG 2 cut(s) 593, 827
BsaHI GRCGYC 1 cut(s) 737
BsaI GGTCTC 1 cut(s) 1036
BsaJI CCNNGG 2 cut(s) 724, 898
Bsc4I CCNNNNNNNGG 2 cut(s) 397, 410
Bse118I RCCGGY 1 cut(s) 633
Bse1I ACTGG 4 cut(s) 228, 580, 590, 971
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 2 cut(s) 724, 898
BseGI GGATG 5 cut(s) 9, 199, 451, 766, 925
BseLI CCNNNNNNNGG 2 cut(s) 397, 410
BseMII CTCAG 6 cut(s) 57, 99, 302, 552, 669, 1031
BseNI ACTGG 4 cut(s) 228, 580, 590, 971
BseXI GCAGC 2 cut(s) 365, 935
BsgI GTGCAG 1 cut(s) 934
Bsh1285I CGRYCG 1 cut(s) 634
BshFI GGCC 2 cut(s) 325, 427
BshNI GGYRCC 3 cut(s) 387, 400, 736
BsiEI CGRYCG 1 cut(s) 634
BsiHKAI GWGCWC 1 cut(s) 627
BsiHKCI CYCGRG 2 cut(s) 503, 740
BsiSI CCGG 1 cut(s) 634
BslI CCNNNNNNNGG 2 cut(s) 397, 410
BsmAI GTCTC 3 cut(s) 513, 767, 1036
BsmI GAATGC 1 cut(s) 719
BsnI GGCC 2 cut(s) 325, 427
Bso31I GGTCTC 1 cut(s) 1036
BsoBI CYCGRG 2 cut(s) 503, 740
Bsp1286I GDGCHC 1 cut(s) 627
Bsp143I GATC 3 cut(s) 534, 747, 795
BspACI CCGC 2 cut(s) 88, 693
BspANI GGCC 2 cut(s) 325, 427
BspCNI CTCAG 6 cut(s) 56, 100, 303, 551, 668, 1032
BspHI TCATGA 1 cut(s) 913
BspLI GGNNCC 4 cut(s) 389, 402, 738, 828
BspPI GGATC 2 cut(s) 542, 803
BspQI GCTCTTC 1 cut(s) 632
BspT107I GGYRCC 3 cut(s) 387, 400, 736
BspTNI GGTCTC 1 cut(s) 1036
BsrFI RCCGGY 1 cut(s) 633
BsrI ACTGG 4 cut(s) 228, 580, 590, 971
BssAI RCCGGY 1 cut(s) 633
BssECI CCNNGG 2 cut(s) 724, 898
BssMI GATC 3 cut(s) 534, 747, 795
BssNI GRCGYC 1 cut(s) 737
BssT1I CCWWGG 2 cut(s) 724, 898
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 4 cut(s) 52, 268, 788, 921
Bst6I CTCTTC 4 cut(s) 411, 632, 765, 1071
BstACI GRCGYC 1 cut(s) 737
BstC8I GCNNGC 2 cut(s) 872, 1008
BstDEI CTNAG 7 cut(s) 43, 108, 311, 538, 655, 978, 1040
BstF5I GGATG 5 cut(s) 9, 199, 451, 766, 925
BstH2I RGCGCY 1 cut(s) 740
BstHHI GCGC 1 cut(s) 739
BstKTI GATC 3 cut(s) 537, 750, 798
BstMAI GTCTC 3 cut(s) 513, 767, 1036
BstMBI GATC 3 cut(s) 534, 747, 795
BstMCI CGRYCG 1 cut(s) 634
BstMWI GCNNNNNNNGC 1 cut(s) 1069
BstNI CCWGG 1 cut(s) 234
BstNSI RCATGY 1 cut(s) 1020
BstSCI CCNGG 1 cut(s) 232
BstV1I GCAGC 2 cut(s) 365, 935
BstX2I RGATCY 1 cut(s) 534
BstXI CCANNNNNNTGG 1 cut(s) 55
BstYI RGATCY 1 cut(s) 534
BsuRI GGCC 2 cut(s) 325, 427
BtsCI GGATG 5 cut(s) 9, 199, 451, 766, 925
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 3 cut(s) 573, 687, 1037
Cac8I GCNNGC 2 cut(s) 872, 1008
CaiI CAGNNNCTG 1 cut(s) 948
CciI TCATGA 1 cut(s) 913
CfoI GCGC 1 cut(s) 739
Cfr10I RCCGGY 1 cut(s) 633
Cfr13I GGNCC 2 cut(s) 202, 425
Csp6I GTAC 2 cut(s) 35, 333
CviAII CATG 5 cut(s) 143, 599, 914, 1012, 1017
CviQI GTAC 2 cut(s) 35, 333
DdeI CTNAG 7 cut(s) 43, 108, 311, 538, 655, 978, 1040
DinI GGCGCC 1 cut(s) 738
DpnI GATC 3 cut(s) 536, 749, 797
DpnII GATC 3 cut(s) 534, 747, 795
Eam1104I CTCTTC 4 cut(s) 411, 632, 765, 1071
EarI CTCTTC 4 cut(s) 411, 632, 765, 1071
Eco130I CCWWGG 2 cut(s) 724, 898
Eco147I AGGCCT 1 cut(s) 325
Eco31I GGTCTC 1 cut(s) 1036
Eco47I GGWCC 1 cut(s) 202
Eco57I CTGAAG 2 cut(s) 158, 850
Eco88I CYCGRG 2 cut(s) 503, 740
EcoO109I RGGNCCY 1 cut(s) 202
EcoRI GAATTC 1 cut(s) 909
EcoRII CCWGG 1 cut(s) 232
EcoT14I CCWWGG 2 cut(s) 724, 898
EgeI GGCGCC 1 cut(s) 738
EheI GGCGCC 1 cut(s) 738
ErhI CCWWGG 2 cut(s) 724, 898
FaeI CATG 5 cut(s) 146, 602, 917, 1015, 1020
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FatI CATG 5 cut(s) 142, 598, 913, 1011, 1016
FbaI TGATCA 1 cut(s) 747
Fnu4HI GCNGC 4 cut(s) 88, 379, 693, 949
FokI GGATG 4 cut(s) 206, 438, 773, 912
Fsp4HI GCNGC 4 cut(s) 88, 379, 693, 949
FspBI CTAG 2 cut(s) 291, 1090
GlaI GCGC 1 cut(s) 738
GluI GCNGC 4 cut(s) 88, 379, 693, 949
GsuI CTGGAG 1 cut(s) 216
HaeII RGCGCY 1 cut(s) 740
HaeIII GGCC 2 cut(s) 325, 427
HapII CCGG 1 cut(s) 634
HhaI GCGC 1 cut(s) 739
Hin1I GRCGYC 1 cut(s) 737
Hin1II CATG 5 cut(s) 146, 602, 917, 1015, 1020
Hin6I GCGC 1 cut(s) 737
HinP1I GCGC 1 cut(s) 737
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 8 cut(s) 28, 296, 364, 448, 472, 506, 682, 710
HpaII CCGG 1 cut(s) 634
HphI GGTGA 5 cut(s) 209, 313, 368, 446, 1023
Hpy166II GTNNAC 2 cut(s) 271, 333
Hpy188I TCNGA 3 cut(s) 217, 447, 658
Hpy188III TCNNGA 6 cut(s) 191, 503, 679, 793, 914, 929
Hpy8I GTNNAC 2 cut(s) 271, 333
HpyAV CCTTC 3 cut(s) 683, 817, 1038
HpyCH4III ACNGT 4 cut(s) 52, 268, 788, 921
HpyCH4V TGCA 8 cut(s) 68, 286, 338, 381, 422, 764, 893, 951
HpyF10VI GCNNNNNNNGC 1 cut(s) 1069
HpyF3I CTNAG 7 cut(s) 43, 108, 311, 538, 655, 978, 1040
Hsp92I GRCGYC 1 cut(s) 737
Hsp92II CATG 5 cut(s) 146, 602, 917, 1015, 1020
HspAI GCGC 1 cut(s) 737
KasI GGCGCC 1 cut(s) 736
Ksp22I TGATCA 1 cut(s) 747
Kzo9I GATC 3 cut(s) 534, 747, 795
LguI GCTCTTC 1 cut(s) 632
LmnI GCTCC 1 cut(s) 235
Lsp1109I GCAGC 2 cut(s) 365, 935
LweI GCATC 2 cut(s) 390, 751
MaeI CTAG 2 cut(s) 291, 1090
MaeIII GTNAC 3 cut(s) 293, 810, 973
MalI GATC 3 cut(s) 536, 749, 797
MboI GATC 3 cut(s) 534, 747, 795
MflI RGATCY 1 cut(s) 534
MhlI GDGCHC 1 cut(s) 627
MluCI AATT 6 cut(s) 210, 394, 525, 594, 909, 995
Mly113I GGCGCC 1 cut(s) 737
MlyI GAGTC 3 cut(s) 290, 481, 515
MmeI TCCRAC 1 cut(s) 279
MroXI GAANNNNTTC 1 cut(s) 363
MseI TTAA 1 cut(s) 74
MslI CAYNNNNRTG 1 cut(s) 147
MspI CCGG 1 cut(s) 634
MspR9I CCNGG 1 cut(s) 234
Mva1269I GAATGC 1 cut(s) 719
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 1 cut(s) 1069
NarI GGCGCC 1 cut(s) 737
NdeII GATC 3 cut(s) 534, 747, 795
NlaIII CATG 5 cut(s) 146, 602, 917, 1015, 1020
NlaIV GGNNCC 4 cut(s) 389, 402, 738, 828
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 1 cut(s) 1020
PagI TCATGA 1 cut(s) 913
PceI AGGCCT 1 cut(s) 325
PciI ACATGT 1 cut(s) 1016
PciSI GCTCTTC 1 cut(s) 632
PctI GAATGC 1 cut(s) 719
PdmI GAANNNNTTC 1 cut(s) 363
PfeI GAWTC 5 cut(s) 28, 364, 448, 682, 710
PfoI TCCNGGA 1 cut(s) 232
PkrI GCNGC 4 cut(s) 89, 380, 694, 950
PleI GAGTC 3 cut(s) 290, 480, 514
PluTI GGCGCC 1 cut(s) 740
PpsI GAGTC 3 cut(s) 290, 480, 514
PpuMI RGGWCCY 1 cut(s) 202
PscI ACATGT 1 cut(s) 1016
Psp5II RGGWCCY 1 cut(s) 202
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 4 cut(s) 389, 402, 738, 828
PspPI GGNCC 2 cut(s) 202, 425
PspPPI RGGWCCY 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 948
PsuI RGATCY 1 cut(s) 534
RsaI GTAC 2 cut(s) 36, 334
RsaNI GTAC 2 cut(s) 35, 333
RseI CAYNNNNRTG 1 cut(s) 147
SapI GCTCTTC 1 cut(s) 632
SaqAI TTAA 1 cut(s) 74
SatI GCNGC 4 cut(s) 88, 379, 693, 949
Sau3AI GATC 3 cut(s) 534, 747, 795
Sau96I GGNCC 2 cut(s) 202, 425
SchI GAGTC 3 cut(s) 290, 481, 515
ScrFI CCNGG 1 cut(s) 234
SduI GDGCHC 1 cut(s) 627
SfaNI GCATC 2 cut(s) 390, 751
SfoI GGCGCC 1 cut(s) 738
SinI GGWCC 1 cut(s) 202
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 2 cut(s) 608, 806
SmoI CTYRAG 2 cut(s) 608, 806
SpeI ACTAGT 1 cut(s) 290
Sse9I AATT 6 cut(s) 210, 394, 525, 594, 909, 995
SseBI AGGCCT 1 cut(s) 325
SsiI CCGC 2 cut(s) 88, 693
SspDI GGCGCC 1 cut(s) 736
SspMI CTAG 2 cut(s) 291, 1090
StuI AGGCCT 1 cut(s) 325
StyD4I CCNGG 1 cut(s) 232
StyI CCWWGG 2 cut(s) 724, 898
TaaI ACNGT 4 cut(s) 52, 268, 788, 921
TaqI TCGA 2 cut(s) 630, 1074
TasI AATT 6 cut(s) 210, 394, 525, 594, 909, 995
TatI WGTACW 1 cut(s) 332
TauI GCSGC 2 cut(s) 90, 695
TfiI GAWTC 5 cut(s) 28, 364, 448, 682, 710
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 3 cut(s) 580, 694, 1044
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 2 cut(s) 378, 948
Tsp45I GTSAC 1 cut(s) 293
TspGWI ACGGA 1 cut(s) 444
TspRI CASTG 3 cut(s) 580, 694, 1044
VpaK11BI GGWCC 1 cut(s) 202
XapI RAATTY 1 cut(s) 909
XceI RCATGY 1 cut(s) 1020
XmnI GAANNNNTTC 1 cut(s) 363
XspI CTAG 2 cut(s) 291, 1090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.