RchiOBHm_Chr1g0373821

Kinase-like protein TMKL1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0013548
Physical Location & Seq
Forward (+)
61844536 .. 61847516
2981 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59766

Sequence Viewer

Length: 1089 bp
ATGAAGAACATCTACAAGCTCACTTTGATTCTGGTACCGACCACAGCCACAGTTTTGGTATTTCTTGCATTTTTTATCTACTTTTGCTGCAAAAGAAGTCCAAAAACTGAGCTAAAAGACGTAGAAAGCACAGAGCTGAAGCATGAAGATGATGGTGTTGTTCTTCAACTGGAGGACTTGATAACTTTTCAGAATGGTGAGGACCTCACAATTTCTGACATACTGGAAGCTCCAGGAGAAGTGATTGGAAAATCCAACTATGGCACACTTTACAAGGCTTTGTTGCAGACTAGTGACTCGGTGAAGTTGCTCAGGTTTTTGAGGCCTGTGTGTACTGCAAGAGTTGAAGATTTTGGTGAAGTGATTCAGCTTTTGGGCTGCATCAGGCACCCCAATTTGGTGCCTCTTCTGGGTTTCTATGCAGGGCCAAGAGGTGAGAAGCTTCTGATTCATCCGTATTATTGGCGTGGGAGTCTGGCTCCATTTGTGAGAGAGGGCAATCCTGAGTCTCACAAATGGGCGATAATTTATAGGATCTCAGTTGGTATAGCCAGAGGATTACATCATCTTCACACTGGCTTGGAGAAGCCAGTAATTCATGGTAATCTCAAGTTGAAAAATGTGCTATTAGACCGGCATTACCAACCTTTCATCTCAGATTTCAGCCTACACCTTCTCTTGAATCCCACTGCTGGCCAAGAAATGCTTGAATCTTCAGCATTCCAAGGCAAGGCGCCCGAGCTGATCAAAATGAGGGATGCAAATGAAGAGACTGATATATACAGTTTCGGGATCATCTTACTTGAGTTACTCACAGGGAAGGAACCCATCAACCAAAACCCTACAACACCTGATGAGGACTTCAGTTTGCCGAACCTAATGAGAAATGCAGTCCTTGGACATAGAATTCATGAAGTGTTCCATCCAGATATCCTTGTCAGTAGCTGCACTACTGATGATGAAATCCCAGTTACTAAGGAAAGAATACTCAAATTGTTTCAGCTTGCTATGACATGTTGTTCTCCTTCACCATCACTGAGACCAAACACCAAGCAAGTGCTATGGAAGCTTGAAGAGATTGGAAGCTAG

Protein Analysis

362

Amino Acids

40.86

Weight (kDa)

6.02

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 80 - 356 6.9e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 80 - 354 1.3e-23 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0013548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07620
fragaria_vesca FvH4_7g27020
malus_domestica MD01G1177800.v1.1 MD07G1249600.v1.1
prunus_persica Prupe.2G273300_v2.0.a1
pyrus_communis pycom01g18860 pycom07g22290 pycom07g22300
rosa_chinensis RchiOBHm_Chr1g0373821
rosa_laevigata RLG00000026803
rosa_multiflora Rmu_sc0013735.1_g000009
rosa_roxburghii Rroxscaffold_4G00283980
rosa_rugosa Rorug01G0379500
rosa_samantha Rh1AG389000 Rh1BG353200 Rh1CG366400 Rh1DG384100
rosa_wichuraiana Rw1G034620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 34
AccB1I GGYRCC 4 cut(s) 34, 387, 400, 733
AclWI GGATC 2 cut(s) 542, 800
AcoI YGGCCR 1 cut(s) 694
AcsI RAATTY 1 cut(s) 906
AcuI CTGAAG 3 cut(s) 158, 699, 847
AcyI GRCGYC 1 cut(s) 734
AfaI GTAC 2 cut(s) 36, 334
AfiI CCNNNNNNNGG 4 cut(s) 397, 410, 692, 730
AflIII ACRYGT 1 cut(s) 1013
AgsI TTSAA 6 cut(s) 167, 347, 616, 682, 710, 1073
AhlI ACTAGT 1 cut(s) 290
AjnI CCWGG 1 cut(s) 232
Alw26I GTCTC 3 cut(s) 513, 764, 1033
AlwI GGATC 2 cut(s) 542, 800
AlwNI CAGNNNCTG 1 cut(s) 945
Ama87I CYCGRG 1 cut(s) 737
AoxI GGCC 3 cut(s) 323, 425, 694
ApeKI GCWGC 3 cut(s) 87, 378, 945
ApoI RAATTY 1 cut(s) 906
Asp700I GAANNNNTTC 1 cut(s) 363
Asp718I GGTACC 1 cut(s) 34
AspLEI GCGC 1 cut(s) 736
AspS9I GGNCC 2 cut(s) 202, 425
AsuHPI GGTGA 5 cut(s) 209, 313, 368, 446, 1020
AvaI CYCGRG 1 cut(s) 737
AvaII GGWCC 1 cut(s) 202
BalI TGGCCA 1 cut(s) 696
BanI GGYRCC 4 cut(s) 34, 387, 400, 733
BarI GAAGNNNNNNTAC 1 cut(s) 28
BbvI GCAGC 3 cut(s) 74, 365, 932
BccI CCATC 4 cut(s) 146, 836, 930, 1039
BciT130I CCWGG 1 cut(s) 234
BclI TGATCA 1 cut(s) 744
BcoDI GTCTC 3 cut(s) 513, 764, 1033
BcuI ACTAGT 1 cut(s) 290
BfaI CTAG 2 cut(s) 291, 1087
BfoI RGCGCY 1 cut(s) 737
BisI GCNGC 3 cut(s) 88, 379, 946
BlsI GCNGC 3 cut(s) 89, 380, 947
Bme1390I CCNGG 1 cut(s) 234
Bme18I GGWCC 1 cut(s) 202
BmeT110I CYCGRG 1 cut(s) 737
BmgT120I GGNCC 2 cut(s) 202, 425
BmiI GGNNCC 6 cut(s) 36, 389, 402, 480, 735, 825
BmrFI CCNGG 1 cut(s) 234
BmrI ACTGGG 1 cut(s) 962
BmsI GCATC 2 cut(s) 390, 748
BmuI ACTGGG 1 cut(s) 962
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpmI CTGGAG 2 cut(s) 191, 216
Bpu10I CCTNAGC 1 cut(s) 311
BpuEI CTTGAG 2 cut(s) 593, 824
BsaHI GRCGYC 1 cut(s) 734
BsaI GGTCTC 1 cut(s) 1033
BsaJI CCNNGG 2 cut(s) 724, 895
Bsc4I CCNNNNNNNGG 4 cut(s) 397, 410, 692, 730
Bse118I RCCGGY 1 cut(s) 633
Bse1I ACTGG 5 cut(s) 174, 228, 580, 590, 968
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 2 cut(s) 724, 895
BseGI GGATG 3 cut(s) 451, 763, 922
BseLI CCNNNNNNNGG 4 cut(s) 397, 410, 692, 730
BseMII CTCAG 6 cut(s) 99, 325, 495, 552, 669, 1028
BseNI ACTGG 5 cut(s) 174, 228, 580, 590, 968
BseXI GCAGC 3 cut(s) 74, 365, 932
BsgI GTGCAG 1 cut(s) 931
BshFI GGCC 3 cut(s) 325, 427, 696
BshNI GGYRCC 4 cut(s) 34, 387, 400, 733
BsiHKCI CYCGRG 1 cut(s) 737
BsiSI CCGG 1 cut(s) 634
BslI CCNNNNNNNGG 4 cut(s) 397, 410, 692, 730
BsmAI GTCTC 3 cut(s) 513, 764, 1033
BsmI GAATGC 1 cut(s) 719
BsnI GGCC 3 cut(s) 325, 427, 696
Bso31I GGTCTC 1 cut(s) 1033
BsoBI CYCGRG 1 cut(s) 737
Bsp143I GATC 3 cut(s) 534, 744, 792
BspANI GGCC 3 cut(s) 325, 427, 696
BspCNI CTCAG 6 cut(s) 100, 324, 496, 551, 668, 1029
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 6 cut(s) 36, 389, 402, 480, 735, 825
BspPI GGATC 2 cut(s) 542, 800
BspT107I GGYRCC 4 cut(s) 34, 387, 400, 733
BspTNI GGTCTC 1 cut(s) 1033
BsrFI RCCGGY 1 cut(s) 633
BsrI ACTGG 5 cut(s) 174, 228, 580, 590, 968
BssAI RCCGGY 1 cut(s) 633
BssECI CCNNGG 2 cut(s) 724, 895
BssMI GATC 3 cut(s) 534, 744, 792
BssNI GRCGYC 1 cut(s) 734
BssT1I CCWWGG 2 cut(s) 724, 895
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 2 cut(s) 52, 785
Bst6I CTCTTC 3 cut(s) 411, 762, 1068
BstACI GRCGYC 1 cut(s) 734
BstC8I GCNNGC 2 cut(s) 694, 1005
BstDEI CTNAG 7 cut(s) 108, 311, 504, 538, 655, 975, 1037
BstF5I GGATG 3 cut(s) 451, 763, 922
BstH2I RGCGCY 1 cut(s) 737
BstHHI GCGC 1 cut(s) 736
BstKTI GATC 3 cut(s) 537, 747, 795
BstMAI GTCTC 3 cut(s) 513, 764, 1033
BstMBI GATC 3 cut(s) 534, 744, 792
BstMWI GCNNNNNNNGC 1 cut(s) 1066
BstNI CCWGG 1 cut(s) 234
BstNSI RCATGY 1 cut(s) 1017
BstSCI CCNGG 1 cut(s) 232
BstV1I GCAGC 3 cut(s) 74, 365, 932
BstX2I RGATCY 1 cut(s) 534
BstXI CCANNNNNNTGG 1 cut(s) 55
BstYI RGATCY 1 cut(s) 534
BsuRI GGCC 3 cut(s) 325, 427, 696
BtsCI GGATG 3 cut(s) 451, 763, 922
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 3 cut(s) 573, 687, 1034
Cac8I GCNNGC 2 cut(s) 694, 1005
CaiI CAGNNNCTG 1 cut(s) 945
CciI TCATGA 1 cut(s) 910
CfoI GCGC 1 cut(s) 736
Cfr10I RCCGGY 1 cut(s) 633
Cfr13I GGNCC 2 cut(s) 202, 425
Csp6I GTAC 2 cut(s) 35, 333
CviAII CATG 4 cut(s) 143, 599, 911, 1014
CviQI GTAC 2 cut(s) 35, 333
DdeI CTNAG 7 cut(s) 108, 311, 504, 538, 655, 975, 1037
DinI GGCGCC 1 cut(s) 735
DpnI GATC 3 cut(s) 536, 746, 794
DpnII GATC 3 cut(s) 534, 744, 792
EaeI YGGCCR 1 cut(s) 694
Eam1104I CTCTTC 3 cut(s) 411, 762, 1068
EarI CTCTTC 3 cut(s) 411, 762, 1068
Eco130I CCWWGG 2 cut(s) 724, 895
Eco147I AGGCCT 1 cut(s) 325
Eco31I GGTCTC 1 cut(s) 1033
Eco32I GATATC 1 cut(s) 931
Eco47I GGWCC 1 cut(s) 202
Eco57I CTGAAG 3 cut(s) 158, 699, 847
Eco88I CYCGRG 1 cut(s) 737
EcoO109I RGGNCCY 1 cut(s) 202
EcoRI GAATTC 1 cut(s) 906
EcoRII CCWGG 1 cut(s) 232
EcoRV GATATC 1 cut(s) 931
EcoT14I CCWWGG 2 cut(s) 724, 895
EgeI GGCGCC 1 cut(s) 735
EheI GGCGCC 1 cut(s) 735
ErhI CCWWGG 2 cut(s) 724, 895
FaeI CATG 4 cut(s) 146, 602, 914, 1017
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FatI CATG 4 cut(s) 142, 598, 910, 1013
FbaI TGATCA 1 cut(s) 744
Fnu4HI GCNGC 3 cut(s) 88, 379, 946
FokI GGATG 3 cut(s) 438, 770, 909
Fsp4HI GCNGC 3 cut(s) 88, 379, 946
FspBI CTAG 2 cut(s) 291, 1087
GlaI GCGC 1 cut(s) 735
GluI GCNGC 3 cut(s) 88, 379, 946
GsuI CTGGAG 2 cut(s) 191, 216
HaeII RGCGCY 1 cut(s) 737
HaeIII GGCC 3 cut(s) 325, 427, 696
HapII CCGG 1 cut(s) 634
HhaI GCGC 1 cut(s) 736
Hin1I GRCGYC 1 cut(s) 734
Hin1II CATG 4 cut(s) 146, 602, 914, 1017
Hin6I GCGC 1 cut(s) 734
HinP1I GCGC 1 cut(s) 734
HindIII AAGCTT 2 cut(s) 440, 1067
HinfI GANTC 8 cut(s) 28, 296, 364, 448, 472, 506, 682, 710
HpaII CCGG 1 cut(s) 634
HphI GGTGA 5 cut(s) 209, 313, 368, 446, 1020
Hpy166II GTNNAC 1 cut(s) 333
Hpy188I TCNGA 4 cut(s) 192, 217, 447, 658
Hpy188III TCNNGA 5 cut(s) 503, 679, 790, 911, 926
Hpy8I GTNNAC 1 cut(s) 333
HpyAV CCTTC 3 cut(s) 683, 814, 1035
HpyCH4III ACNGT 2 cut(s) 52, 785
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 9 cut(s) 68, 90, 286, 338, 381, 422, 761, 890, 948
HpyF10VI GCNNNNNNNGC 1 cut(s) 1066
HpyF3I CTNAG 7 cut(s) 108, 311, 504, 538, 655, 975, 1037
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 1 cut(s) 734
Hsp92II CATG 4 cut(s) 146, 602, 914, 1017
HspAI GCGC 1 cut(s) 734
KasI GGCGCC 1 cut(s) 733
KpnI GGTACC 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 744
Kzo9I GATC 3 cut(s) 534, 744, 792
LmnI GCTCC 2 cut(s) 235, 484
Lsp1109I GCAGC 3 cut(s) 74, 365, 932
LweI GCATC 2 cut(s) 390, 748
MaeI CTAG 2 cut(s) 291, 1087
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 3 cut(s) 293, 807, 970
MalI GATC 3 cut(s) 536, 746, 794
MboI GATC 3 cut(s) 534, 744, 792
MboII GAAGA 9 cut(s) 16, 155, 158, 359, 398, 560, 705, 779, 1085
MflI RGATCY 1 cut(s) 534
MlsI TGGCCA 1 cut(s) 696
MluCI AATT 6 cut(s) 210, 394, 525, 594, 906, 992
MluNI TGGCCA 1 cut(s) 696
Mly113I GGCGCC 1 cut(s) 734
MlyI GAGTC 3 cut(s) 290, 481, 515
MmeI TCCRAC 1 cut(s) 279
Mox20I TGGCCA 1 cut(s) 696
MroXI GAANNNNTTC 1 cut(s) 363
MscI TGGCCA 1 cut(s) 696
MslI CAYNNNNRTG 1 cut(s) 147
Msp20I TGGCCA 1 cut(s) 696
MspI CCGG 1 cut(s) 634
MspR9I CCNGG 1 cut(s) 234
Mva1269I GAATGC 1 cut(s) 719
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 1 cut(s) 1066
NarI GGCGCC 1 cut(s) 734
NdeII GATC 3 cut(s) 534, 744, 792
NlaIII CATG 4 cut(s) 146, 602, 914, 1017
NlaIV GGNNCC 6 cut(s) 36, 389, 402, 480, 735, 825
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 1 cut(s) 1017
PagI TCATGA 1 cut(s) 910
PceI AGGCCT 1 cut(s) 325
PciI ACATGT 1 cut(s) 1013
PctI GAATGC 1 cut(s) 719
PdmI GAANNNNTTC 1 cut(s) 363
PfeI GAWTC 5 cut(s) 28, 364, 448, 682, 710
PfoI TCCNGGA 1 cut(s) 232
PkrI GCNGC 3 cut(s) 89, 380, 947
PleI GAGTC 3 cut(s) 290, 480, 514
PluTI GGCGCC 1 cut(s) 737
PpsI GAGTC 3 cut(s) 290, 480, 514
PpuMI RGGWCCY 1 cut(s) 202
PscI ACATGT 1 cut(s) 1013
Psp5II RGGWCCY 1 cut(s) 202
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 6 cut(s) 36, 389, 402, 480, 735, 825
PspPI GGNCC 2 cut(s) 202, 425
PspPPI RGGWCCY 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 945
PsuI RGATCY 1 cut(s) 534
RsaI GTAC 2 cut(s) 36, 334
RsaNI GTAC 2 cut(s) 35, 333
RseI CAYNNNNRTG 1 cut(s) 147
SatI GCNGC 3 cut(s) 88, 379, 946
Sau3AI GATC 3 cut(s) 534, 744, 792
Sau96I GGNCC 2 cut(s) 202, 425
SchI GAGTC 3 cut(s) 290, 481, 515
ScrFI CCNGG 1 cut(s) 234
SfaNI GCATC 2 cut(s) 390, 748
SfoI GGCGCC 1 cut(s) 735
SinI GGWCC 1 cut(s) 202
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 2 cut(s) 608, 803
SmoI CTYRAG 2 cut(s) 608, 803
SpeI ACTAGT 1 cut(s) 290
Sse9I AATT 6 cut(s) 210, 394, 525, 594, 906, 992
SseBI AGGCCT 1 cut(s) 325
SspDI GGCGCC 1 cut(s) 733
SspMI CTAG 2 cut(s) 291, 1087
StuI AGGCCT 1 cut(s) 325
StyD4I CCNGG 1 cut(s) 232
StyI CCWWGG 2 cut(s) 724, 895
TaaI ACNGT 2 cut(s) 52, 785
TaiI ACGT 1 cut(s) 123
TasI AATT 6 cut(s) 210, 394, 525, 594, 906, 992
TatI WGTACW 1 cut(s) 332
TfiI GAWTC 5 cut(s) 28, 364, 448, 682, 710
TscAI CASTG 3 cut(s) 580, 694, 1041
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 3 cut(s) 87, 378, 945
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 9 cut(s) 17, 159, 440, 587, 640, 780, 899, 927, 975
TspGWI ACGGA 1 cut(s) 444
TspRI CASTG 3 cut(s) 580, 694, 1041
VpaK11BI GGWCC 1 cut(s) 202
XapI RAATTY 1 cut(s) 906
XceI RCATGY 1 cut(s) 1017
XmnI GAANNNNTTC 1 cut(s) 363
XspI CTAG 2 cut(s) 291, 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.