Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0012974

Physical Location & Seq

Genomic Coordinates Forward (+)

25149578 .. 25154223

Gene Structure 4646 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4422 bp

ATGTCACATGACATGCAAAAAAACAGAACCCGTATTTTCCGAAGCACCTGCACGTTTGAAAATACAAAAGATATGGCACTGGTGGATCTATATGTTAAATCTAGGAAACATATTGAGGTCAGACCCAAATTCAAGAAATATGTGGGTCACAAAAGGGGCTCATTTGCAAATTTAACAGAATCGCCGTTACGAGAGTACAAGTCACGTGAGCGGTGCATTTCTTCTCCTGACCGAATTAATAACACTCCGTCGTCCTCGTCTTCGATCTTTTATGGCCTCCTTGGCAAACACACAACTCGCAGAGAAAAGCTGTGCCCCTCTCTCCACCGCTATTTCTCTCTGCAAAACACAGGTTCCGACCCCTCTTTCTTCTTCTCAAATTGCCCTTTCATGGCGTCAATAAAGCCTCTGTTTTTAGTTATTTTCATAGTTTTTGAGTCCCTCTGTCTACTCGCCAATGGCGTTTCCCACCAAAACCCAGAACAGGAAGCCCTTCTTTCCTTCAAAACCGCCCTCGGAAATCCCGCAGTGCTCTCCTCGTGGCGGTCCTCCGCCTCGCGCTGCGACTGGGTTGGCGTTTCCTGCCATTTCGGCCGAGTCACCGAGCTCGCCCTCCCGTCCCTCTCCCTCAAAGGCCCGCTCCCTCCCTCCCTCTTCTCCCTCTCCGACCTCGCCGTGCTGGACCTCTCCTCCAACCTACTTCACGGCAAAATTCCACCTCAAATTCTCAACCTCAAACACCTAAACCACCTCTGCCTCACTGGTAACCAGCTCTCCGGCGAAATACCGACCCAAATCGTAGAGTTGACTGAGCTGGAGACCCTGAAGCTCGGAACCAACCTTTTCACCGGGAAAATCCCGCCGGAGCTGGGATATTTATCTCTGCTTCAAACCCTGGAGCTCTCCAGTAATGACTTCTATGGAGATATTCCGACCCAGATTGGAAATTTGACCCGGCTGAAGTTCCTGGACCTGGGCAACAACGCCCTCTCAGGTTCTCTTCCCTCTGACCTGTTTCCAAAGCTTCAATCTTTGGCTTCCATGGACGTATCACGCAATTCACTCTCTGGGGCAATTCCACCGTCAATTGGTAGCCTGAAGAATCTCACGGACCTCTACATTGGCAGCAATCATATCTCCGGCCCGTTGCCCCGCGAAATCGGAGAGCTTTCGCGGCTCGAAATTTTGGACGCTGCTTCCTGTTCCATAACAGGGCCGTTGCCGGAAGAGATTTCTAAGCTGGAATCGTTGACCAAAATTGACCTCTCGTACAACCCATTGAGGTGTTCGTTCCCAAAAGGCGTAGGGAAGTTGCAGAACCTGAGTCTTTTGGCTTTGGTTTTCACAGAGCTTAATGGCTCTGTTCCTGCAGAGCTAGGGAACTGTAAAAGTTTGACGACTTTGATGCTTTCGTTCAATTCGCTTTCCGGGCCGTTGCCTCAAGAGCTCTCTGAGCTTCCACTGATGACTTTTTCCGTTGAAAAGAACCAGCTTTCGGGCCTCTTGCCTTCCTGGCTCGGAAAATTGAAGCAAGTCGAATCGCTTCTGCTTTCGAGCAATCTGTTTTCCGGGAAAATTCCACCGGAGATTGGCAACTGCTCTATGCTGAGGTTACTTGGCTTGAGCAATAACTTGTTGACGGGTCCGATTCCGGAAGAGATTTGCAATGCGGGTTCGCTTTTGGAGATTGATCTCGATAGCAATTACCTCTCGGGAACAATTGACAGCACGTTTGTCAAGTGCAAGAACTTGACGCAGCTCAACTTGGTGAACAATCGGATTGCCGGCGCAATCCCTCATTACCTTTCGGAGCTTCCACTCATGGTGATCGACCTTGATTCGAACAACTTCACGGGCACAATACCGAAAAGTCTTTGGAATTCGGAGAATTTGATGGAGTTCACAGCTTCGAACAATCAGCTGAGCGGTTCAATTTCGAATGAGATTGGTCGGGCAGCTGTCTTGGAAAGGCTCGTCCTTAGTAACAATCGGCTGACAGGAAAACTTCCTAAAGAGATCGGAAATGTCAGCAGCCTTTCTGTTCTAATATTGAATTCGAATCTTCTGGAAGGGAACATTCCGGCGGAGCTTAGCAAGTGCACCGGTCTTACTACATTGGATCTGGGAAGCAATCAGCTTAGCGGCTCTATCCCAACAGAGCTATCAGAGCTAAGCCAATTGCAATGTCTGGTACTTTCTCACAACAATCTATCTGGTTCAATTCCTTTGAAAAAATCATTGTATTTTCGACAAGCAAATATCCCGGTTTTGAGCTTTGTGCAACATGTTGGAGTGTTTGATCTGTCCTACAATAGGTTGTCGGGTGCAATACCTGAAGATTTGGGTGACTCTGTTATTCTGGTGGATGTTTTGATCAGCAACAACCTGCTCTCTGGTGAAATTCCAAGGTCCCTCGCTCGTTTAACTAACCTTACAACTTTGGATTTGTCTGGGAACATGCTCAGTGGTTCCATTCCTCCTGAGTTTGGTGATTCGCCGAAGCTTCAAGGCTTGAATCTGGGAAATAACCAGCTTACGAGTACCATCCCTGGAAGCTTTGGTCGTTTAGGAAGCTTGGTGAAGCTTAATTTGACTGGTAATAAGTTATCTGGTTCTGTACCTGTGAGCTTTGGGGAATTGAAAGAGCTAACTCACTTGGATTTAAGCTCGAACAAGCTAGATGGCGAGCTTCCTTCATCGCTGTCAAGCATGCAGAATCTCGTGGGGCTGTATGTTCAGGAGAACCGGCTTAAAGGTGGTTTTGATAAGCTGTTCTCGAATACGATAGCTTGGAGGATCGAAAAGATGAACTTGAGCAATAATTTCTTTGATGGGGTGCTGCCCCATTCTCTTGGTAATCTGTCATACTTGACGGATTTCGATCTTCATTCAAATTCGTTCCAAGGAGAGATTCCTCCAGACCTTGGGAATTTGATGCAACTTGAGTATTTTGATGTTTCAAGTAACAAGCTCTCTGGACAGATTCCAGAGAAAATTTGCAGCCTCATCAATCTGTTTTACGTGAACTTGGCAGAAAATAGGTTGGAAGGGCCGATTCCTAATAGTGGTATTTGCCGTAACCTGTCAGAAAAATCGCTTGCCGGTAACAAGAAACTGTGTGGGAGAAGTAAAGATTTGGATTGCCATGCCAAGAGTTTTGATGAATCAGCATTGTTTAATGCCCGGGGAGTTGCTGCTATTGTAGTTGGAAGTGCTCTAATCTTTATTGTTGCAGCTTATGCTGTAGTAAGATGGATCGACCCGGAGAAGTTGACTAGTTTCATGGAACATTATTTTCTCAGTAGCAGCAGATCACAGGAGCTTCTGAGCATCAACGTTGCCATGTCTCAGCAGCCGCTTCTCAAGCTAACTTTAGTTGACATTCTTGAAGCCACCAACAACTTCTGCAAGACAAACATAATAGGAGATGGAGGCTTTGGAACCGTGTACAAGGCCACGTTGTCGGATGGGAAAACTGTTGCGGTTAAGAAGCTAAATGAGTATAAAACTCAGGGACATCGAGAATTCCTTGCCGAAATGGAAACCCTGGGGAACGTAAACCACCAAAATCTCGTTCCTTTACTCGGGTACTGTTCTCTTGGCGAGGAGAAACTCCTTGTTTACGAGTATATGGTAAATGGGAGCCTCGACATTTGGCTGAGAAATCGGACTGGAGATCCTGAAGTCCTAGACTGGGACAGACGTTTCAAAATTGCAATGGGTGCAGCCCGCGGCGTGGCTTTTCTTCACCATGGATTCGGCCCCCATATCATCCACAGGGATATCAAAGCCAGCAACATCTTGCTCAACGAAGACTTCGAGCCCAAAGTTGCAGACTTTGGACTTGCAAGATCGATCAATGCCTGTGAGACTCACATTAGTACTGATATAGCCGGAACATTTGGCTACATTCCACCTGAGTATGGACAGAGCGCGCGGTCTACCACAAAGGGAGATGTCTATAGCTTTGGTGTGATACTGCTCGAGTTGGTGACAGGGAAAGAGCCAACCGGACCCGATTTCAAAGATTTGGAAGGCGGGAATTTAGTCGGGTGGGTTTTTCAGATGCTGAAGAAGGGGAAGGCTGCTGATGTTCTTGACCCGATGGTGCTTGACGCGGATTCGAAGCGCACGATGGTTCAAGTGTTGAAGATTGCCTCTGTGTGCGTCTCTGATAACCCAGCTCAGAGGCCTACAATGCTTCAAGTGCTGAAGTTTCTCAAAGGGATTAATGATGATAGGAAGGGACGTAGGGCTTATAAACTAATGTTAATTAAGCACCATTTAGTTTTCATGTGTTTGATGCTTGAGTTTCTTCTATCCATTGATTTTTTCGTAGCAATATCGGAAAATTTAGAGAAATATAAGAAACTTTTAAATTTCGCCCAAAAACGACATATTTCTTTAATATTTCCGACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009556 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010234 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019953 GO:0022402 GO:0022414 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0034293 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0043934 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044703 GO:0045165 GO:0046777 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1903046 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1474

Amino Acids

161.54

Weight (kDa)

6.67

Isoelectric Point (pI)

34.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	159 - 195	2e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	284 - 374	1.3e-07	Leucine-rich repeat region
LRR_14	PF23598	313 - 398	6e-09	Leucine-rich repeat region
LRR_8	PF13855	319 - 379	1.5e-06	Leucine rich repeat
LRR_14	PF23598	364 - 491	6.3e-10	Leucine-rich repeat region
LRR_8	PF13855	829 - 869	2.4e-07	Leucine rich repeat
Pkinase	PF00069	1138 - 1405	2.1e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	1139 - 1406	1.6e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012974)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07280
fragaria_vesca	FvH4_7g29180
malus_domestica	MD01G1198500.v1.1 MD07G1266400.v1.1
prunus_persica	Prupe.2G292000_v2.0.a1
pyrus_communis	pycom01g20870 pycom07g24420
rosa_chinensis	RchiOBHm_Chr1g0376951
rosa_laevigata	RLG00000026557
rosa_multiflora	Rmu_sc0026264.1_g000001 Rmu_ssc0000155.1_g000035
rosa_roxburghii	Rroxscaffold_4G00281610
rosa_rugosa	Rorug01G0399800
rosa_samantha	Rh1AG417000 Rh1BG376400 Rh1CG390200 Rh1DG407100
rosa_wichuraiana	Rw1G036600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4260
AarI	CACCTGC	1 cut(s)	56
Acc36I	ACCTGC	2 cut(s)	56, 2394
AccB7I	CCANNNNNTGG	1 cut(s)	2924
AccBSI	CCGCTC	3 cut(s)	211, 640, 1926
AccI	GTMKAC	2 cut(s)	448, 3941
AccII	CGCG	7 cut(s)	559, 1155, 1174, 3732, 3935, 3937, 4118
AccIII	TCCGGA	1 cut(s)	1651
AclI	AACGTT	1 cut(s)	3336
AclWI	GGATC	5 cut(s)	93, 2127, 2804, 3263, 3671
AcoI	YGGCCR	1 cut(s)	592
AcuI	CTGAAG	7 cut(s)	845, 980, 1118, 2355, 3702, 4091, 4232
AcvI	CACGTG	1 cut(s)	206
AcyI	GRCGYC	1 cut(s)	395
AfaI	GTAC	8 cut(s)	197, 1271, 2193, 2542, 2619, 3449, 3590, 3883
AflIII	ACRYGT	1 cut(s)	2284
AgeI	ACCGGT	1 cut(s)	2102
AhlI	ACTAGT	1 cut(s)	3275
AjnI	CCWGG	6 cut(s)	894, 966, 972, 1511, 2548, 3546
AjuI	GAANNNNNNNTTGG	4 cut(s)	2895, 2927, 3558, 3590
Alw21I	GWGCWC	6 cut(s)	534, 609, 903, 1449, 2102, 3217
Alw26I	GTCTC	4 cut(s)	812, 3351, 3863, 4174
Alw44I	GTGCAC	1 cut(s)	2098
AlwI	GGATC	5 cut(s)	93, 2127, 2804, 3263, 3671
AlwNI	CAGNNNCTG	2 cut(s)	1321, 4069
Ama87I	CYCGRG	4 cut(s)	1711, 3183, 3584, 3983
Aor13HI	TCCGGA	1 cut(s)	1651
ApaLI	GTGCAC	1 cut(s)	2098
ArsI	GACNNNNNNTTYG	2 cut(s)	1715, 1747
AseI	ATTAAT	2 cut(s)	237, 4230
AsiGI	ACCGGT	1 cut(s)	2102
Asp700I	GAANNNNTTC	3 cut(s)	492, 1542, 1847
AspLEI	GCGC	5 cut(s)	561, 1790, 3935, 3937, 4131
AsuC2I	CCSGG	8 cut(s)	850, 955, 1429, 1570, 2264, 3184, 3185, 3263
AsuII	TTCGAA	5 cut(s)	1841, 1910, 1937, 2057, 4124
AvaI	CYCGRG	4 cut(s)	1711, 3183, 3584, 3983
AvaII	GGWCC	7 cut(s)	546, 682, 970, 1111, 1643, 2409, 4013
BaeGI	GKGCMC	3 cut(s)	317, 1859, 2102
BanII	GRGCYC	5 cut(s)	161, 609, 903, 1449, 3825
BauI	CACGAG	2 cut(s)	538, 2720
BbrPI	CACGTG	1 cut(s)	206
BbsI	GAAGAC	2 cut(s)	252, 3819
Bbv12I	GWGCWC	6 cut(s)	534, 609, 903, 1449, 2102, 3217
BbvCI	CCTCAGC	1 cut(s)	1607
BccI	CCATC	9 cut(s)	1888, 2552, 2675, 2825, 3246, 3422, 3461, 4099, 4129
BceAI	ACGGC	6 cut(s)	169, 659, 721, 1201, 1417, 3060
BcgI	CGANNNNNNTGC	4 cut(s)	30, 64, 1387, 1421
BciT130I	CCWGG	6 cut(s)	896, 968, 974, 1513, 2550, 3548
BclI	TGATCA	1 cut(s)	2373
BcnI	CCSGG	8 cut(s)	850, 955, 1429, 1570, 2264, 3184, 3185, 3263
BcoDI	GTCTC	4 cut(s)	812, 3351, 3863, 4174
BcuI	ACTAGT	1 cut(s)	3275
BfaI	CTAG	5 cut(s)	102, 1376, 2678, 3276, 3689
BfmI	CTRYAG	3 cut(s)	1368, 3243, 3961
BfuAI	ACCTGC	2 cut(s)	56, 2394
BglI	GCCNNNNNGGC	3 cut(s)	282, 591, 1513
BlpI	GCTNAGC	4 cut(s)	1922, 2090, 2138, 2171
BmcAI	AGTACT	1 cut(s)	3883
Bme18I	GGWCC	7 cut(s)	546, 682, 970, 1111, 1643, 2409, 4013
BmeT110I	CYCGRG	4 cut(s)	1711, 3183, 3584, 3983
BmiI	GGNNCC	9 cut(s)	355, 835, 1644, 2411, 2470, 3442, 3644, 3763, 4015
BmrI	ACTGGG	2 cut(s)	577, 3703
BmsI	GCATC	5 cut(s)	1395, 2925, 3339, 4056, 4293
BmuI	ACTGGG	2 cut(s)	577, 3703
BpiI	GAAGAC	2 cut(s)	252, 3819
BpmI	CTGGAG	5 cut(s)	836, 889, 917, 2901, 3693
Bpu10I	CCTNAGC	1 cut(s)	1607
Bpu1102I	GCTNAGC	4 cut(s)	1922, 2090, 2138, 2171
Bpu14I	TTCGAA	5 cut(s)	1841, 1910, 1937, 2057, 4124
BpuEI	CTTGAG	6 cut(s)	1425, 1642, 2833, 2963, 3347, 4328
BpuMI	CCSGG	8 cut(s)	850, 955, 1429, 1570, 2264, 3184, 3185, 3263
Bsa29I	ATCGAT	1 cut(s)	3854
BsaAI	YACGTR	2 cut(s)	206, 3022
BsaBI	GATNNNNATC	2 cut(s)	877, 2880
BsaHI	GRCGYC	1 cut(s)	395
BsaI	GGTCTC	1 cut(s)	812
BsaWI	WCCGGW	4 cut(s)	1582, 1651, 2102, 4010
BsaXI	ACNNNNNCTCC	4 cut(s)	674, 704, 758, 788
Bse118I	RCCGGY	4 cut(s)	1784, 2102, 2745, 3101
Bse1I	ACTGG	7 cut(s)	84, 572, 766, 906, 2599, 3676, 3698
Bse3DI	GCAATG	3 cut(s)	1672, 2189, 3723
Bse8I	GATNNNNATC	2 cut(s)	877, 2880
BseAI	TCCGGA	1 cut(s)	1651
BseBI	CCWGG	6 cut(s)	896, 968, 974, 1513, 2550, 3548
BseCI	ATCGAT	1 cut(s)	3854
BseGI	GGATG	4 cut(s)	2371, 2544, 3472, 3771
BseJI	GATNNNNATC	2 cut(s)	877, 2880
BseMI	GCAATG	3 cut(s)	1672, 2189, 3723
BseNI	ACTGG	7 cut(s)	84, 572, 766, 906, 2599, 3676, 3698
BsePI	GCGCGC	1 cut(s)	3933
BseRI	GAGGAG	3 cut(s)	526, 679, 3620
BseSI	GKGCMC	3 cut(s)	317, 1859, 2102
BseX3I	CGGCCG	1 cut(s)	592
BseYI	CCCAGC	2 cut(s)	868, 4180
BsgI	GTGCAG	2 cut(s)	34, 3744
Bsh1236I	CGCG	7 cut(s)	559, 1155, 1174, 3732, 3935, 3937, 4118
Bsh1285I	CGRYCG	1 cut(s)	595
BshTI	ACCGGT	1 cut(s)	2102
BshVI	ATCGAT	1 cut(s)	3854
BsiEI	CGRYCG	1 cut(s)	595
BsiHKAI	GWGCWC	6 cut(s)	534, 609, 903, 1449, 2102, 3217
BsiHKCI	CYCGRG	4 cut(s)	1711, 3183, 3584, 3983
BslFI	GGGAC	6 cut(s)	424, 604, 2395, 3528, 3710, 4260
BsmAI	GTCTC	4 cut(s)	812, 3351, 3863, 4174
BsmBI	CGTCTC	1 cut(s)	4174
BsmFI	GGGAC	6 cut(s)	424, 604, 2395, 3528, 3710, 4260
Bso31I	GGTCTC	1 cut(s)	812
BsoBI	CYCGRG	4 cut(s)	1711, 3183, 3584, 3983
Bsp119I	TTCGAA	5 cut(s)	1841, 1910, 1937, 2057, 4124
Bsp13I	TCCGGA	1 cut(s)	1651
Bsp1407I	TGTACA	1 cut(s)	3447
Bsp1720I	GCTNAGC	4 cut(s)	1922, 2090, 2138, 2171
Bsp19I	CCATGG	2 cut(s)	1041, 3751
BspDI	ATCGAT	1 cut(s)	3854
BspEI	TCCGGA	1 cut(s)	1651
BspFNI	CGCG	7 cut(s)	559, 1155, 1174, 3732, 3935, 3937, 4118
BspLI	GGNNCC	9 cut(s)	355, 835, 1644, 2411, 2470, 3442, 3644, 3763, 4015
BspMAI	CTGCAG	1 cut(s)	1372
BspMI	ACCTGC	2 cut(s)	56, 2394
BspPI	GGATC	5 cut(s)	93, 2127, 2804, 3263, 3671
BspT104I	TTCGAA	5 cut(s)	1841, 1910, 1937, 2057, 4124
BspTNI	GGTCTC	1 cut(s)	812
BsrBI	CCGCTC	3 cut(s)	211, 640, 1926
BsrDI	GCAATG	3 cut(s)	1672, 2189, 3723
BsrFI	RCCGGY	4 cut(s)	1784, 2102, 2745, 3101
BsrGI	TGTACA	1 cut(s)	3447
BsrI	ACTGG	7 cut(s)	84, 572, 766, 906, 2599, 3676, 3698
BssAI	RCCGGY	4 cut(s)	1784, 2102, 2745, 3101
BssHII	GCGCGC	1 cut(s)	3933
BssNI	GRCGYC	1 cut(s)	395
BssSI	CACGAG	2 cut(s)	538, 2720
BssT1I	CCWWGG	6 cut(s)	280, 1041, 2405, 2902, 2923, 3751
Bst2BI	CACGAG	2 cut(s)	538, 2720
Bst2UI	CCWGG	6 cut(s)	896, 968, 974, 1513, 2550, 3548
Bst4CI	ACNGT	6 cut(s)	1083, 1385, 3117, 3445, 3478, 3593
Bst6I	CTCTTC	4 cut(s)	659, 1005, 1221, 1650
BstACI	GRCGYC	1 cut(s)	395
BstAPI	GCANNNNNTGC	2 cut(s)	3239, 3722
BstAUI	TGTACA	1 cut(s)	3447
BstBAI	YACGTR	2 cut(s)	206, 3022
BstBI	TTCGAA	5 cut(s)	1841, 1910, 1937, 2057, 4124
BstC8I	GCNNGC	9 cut(s)	609, 638, 1786, 2687, 2711, 3099, 3730, 3793, 3935
BstDSI	CCRYGG	3 cut(s)	1041, 3730, 3751
BstEII	GGTNACC	1 cut(s)	764
BstENI	CCTNNNNNAGG	1 cut(s)	2311
BstF5I	GGATG	4 cut(s)	2371, 2544, 3472, 3771
BstFNI	CGCG	7 cut(s)	559, 1155, 1174, 3732, 3935, 3937, 4118
BstHHI	GCGC	5 cut(s)	561, 1790, 3935, 3937, 4131
BstMAI	GTCTC	4 cut(s)	812, 3351, 3863, 4174
BstMCI	CGRYCG	1 cut(s)	595
BstNI	CCWGG	6 cut(s)	896, 968, 974, 1513, 2550, 3548
BstNSI	RCATGY	4 cut(s)	16, 2288, 2461, 2713
BstPI	GGTNACC	1 cut(s)	764
BstSFI	CTRYAG	3 cut(s)	1368, 3243, 3961
BstSLI	GKGCMC	3 cut(s)	317, 1859, 2102
BstUI	CGCG	7 cut(s)	559, 1155, 1174, 3732, 3935, 3937, 4118
BstV2I	GAAGAC	2 cut(s)	252, 3819
BstX2I	RGATCY	3 cut(s)	85, 2119, 3676
BstXI	CCANNNNNNTGG	1 cut(s)	2852
BstYI	RGATCY	3 cut(s)	85, 2119, 3676
BstZI	CGGCCG	1 cut(s)	592
Bsu15I	ATCGAT	1 cut(s)	3854
BsuTUI	ATCGAT	1 cut(s)	3854
BtgI	CCRYGG	3 cut(s)	1041, 3730, 3751
BtgZI	GCGATG	1 cut(s)	2682
BtsCI	GGATG	4 cut(s)	2371, 2544, 3472, 3771
BtsI	GCAGTG	1 cut(s)	534
BtsIMutI	CAGTG	5 cut(s)	77, 534, 759, 1460, 2470
BveI	ACCTGC	2 cut(s)	56, 2394
Cac8I	GCNNGC	9 cut(s)	609, 638, 1786, 2687, 2711, 3099, 3730, 3793, 3935
CaiI	CAGNNNCTG	2 cut(s)	1321, 4069
CfoI	GCGC	5 cut(s)	561, 1790, 3935, 3937, 4131
Cfr10I	RCCGGY	4 cut(s)	1784, 2102, 2745, 3101
Cfr42I	CCGCGG	1 cut(s)	3733
Cfr9I	CCCGGG	1 cut(s)	3183
ClaI	ATCGAT	1 cut(s)	3854
CseI	GACGC	5 cut(s)	384, 1199, 1762, 4124, 4156
Csp6I	GTAC	8 cut(s)	196, 1270, 2192, 2541, 2618, 3448, 3589, 3882
CspAI	ACCGGT	1 cut(s)	2102
CviQI	GTAC	8 cut(s)	196, 1270, 2192, 2541, 2618, 3448, 3589, 3882
DraI	TTTAAA	1 cut(s)	4377
EaeI	YGGCCR	1 cut(s)	592
EagI	CGGCCG	1 cut(s)	592
Eam1104I	CTCTTC	4 cut(s)	659, 1005, 1221, 1650
EarI	CTCTTC	4 cut(s)	659, 1005, 1221, 1650
EciI	GGCGGA	2 cut(s)	541, 2099
Ecl136II	GAGCTC	3 cut(s)	607, 901, 1447
EclXI	CGGCCG	1 cut(s)	592
Eco130I	CCWWGG	6 cut(s)	280, 1041, 2405, 2902, 2923, 3751
Eco147I	AGGCCT	1 cut(s)	4192
Eco24I	GRGCYC	5 cut(s)	161, 609, 903, 1449, 3825
Eco31I	GGTCTC	1 cut(s)	812
Eco32I	GATATC	1 cut(s)	3784
Eco47I	GGWCC	7 cut(s)	546, 682, 970, 1111, 1643, 2409, 4013
Eco52I	CGGCCG	1 cut(s)	592
Eco53kI	GAGCTC	3 cut(s)	607, 901, 1447
Eco57I	CTGAAG	7 cut(s)	845, 980, 1118, 2355, 3702, 4091, 4232
Eco72I	CACGTG	1 cut(s)	206
Eco88I	CYCGRG	4 cut(s)	1711, 3183, 3584, 3983
Eco91I	GGTNACC	1 cut(s)	764
EcoICRI	GAGCTC	3 cut(s)	607, 901, 1447
EcoNI	CCTNNNNNAGG	1 cut(s)	2311
EcoO109I	RGGNCCY	1 cut(s)	2409
EcoO65I	GGTNACC	1 cut(s)	764
EcoRI	GAATTC	3 cut(s)	1879, 2053, 3524
EcoRII	CCWGG	6 cut(s)	894, 966, 972, 1511, 2548, 3546
EcoRV	GATATC	1 cut(s)	3784
EcoT14I	CCWWGG	6 cut(s)	280, 1041, 2405, 2902, 2923, 3751
EcoT38I	GRGCYC	5 cut(s)	161, 609, 903, 1449, 3825
ErhI	CCWWGG	6 cut(s)	280, 1041, 2405, 2902, 2923, 3751
Esp3I	CGTCTC	1 cut(s)	4174
FalI	AAGNNNNNCTT	4 cut(s)	480, 512, 2795, 2827
FaqI	GGGAC	6 cut(s)	424, 604, 2395, 3528, 3710, 4260
FauI	CCCGC	7 cut(s)	532, 645, 867, 1160, 1663, 3737, 4031
FbaI	TGATCA	1 cut(s)	2373
FblI	GTMKAC	2 cut(s)	448, 3941
FokI	GGATG	4 cut(s)	2378, 2531, 3479, 3758
FriOI	GRGCYC	5 cut(s)	161, 609, 903, 1449, 3825
FspBI	CTAG	5 cut(s)	102, 1376, 2678, 3276, 3689
GlaI	GCGC	5 cut(s)	560, 1789, 3934, 3936, 4130
GsaI	CCCAGC	2 cut(s)	872, 4184
GsuI	CTGGAG	5 cut(s)	836, 889, 917, 2901, 3693
HgaI	GACGC	5 cut(s)	384, 1199, 1762, 4124, 4156
HhaI	GCGC	5 cut(s)	561, 1790, 3935, 3937, 4131
Hin1I	GRCGYC	1 cut(s)	395
Hin6I	GCGC	5 cut(s)	559, 1788, 3933, 3935, 4129
HinP1I	GCGC	5 cut(s)	559, 1788, 3933, 3935, 4129
HincII	GTYRAC	5 cut(s)	807, 1251, 1638, 3273, 3379
HindII	GTYRAC	5 cut(s)	807, 1251, 1638, 3273, 3379
HindIII	AAGCTT	5 cut(s)	1022, 2501, 2554, 2572, 2582
Hpy99I	CGWCG	1 cut(s)	253
HpyCH4III	ACNGT	6 cut(s)	1083, 1385, 3117, 3445, 3478, 3593
Hsp92I	GRCGYC	1 cut(s)	395
HspAI	GCGC	5 cut(s)	559, 1788, 3933, 3935, 4129
Kpn2I	TCCGGA	1 cut(s)	1651
KroI	GCCGGC	1 cut(s)	1784
KroNI	GCCGGC	1 cut(s)	1786
Ksp22I	TGATCA	1 cut(s)	2373
KspI	CCGCGG	1 cut(s)	3733
LmnI	GCTCC	7 cut(s)	645, 865, 898, 1810, 2086, 3319, 3642
LweI	GCATC	5 cut(s)	1395, 2925, 3339, 4056, 4293
MaeI	CTAG	5 cut(s)	102, 1376, 2678, 3276, 3689
MbiI	CCGCTC	3 cut(s)	211, 640, 1926
MfeI	CAATTG	3 cut(s)	1086, 1719, 2177
MflI	RGATCY	3 cut(s)	85, 2119, 3676
MlyI	GAGTC	5 cut(s)	446, 606, 1333, 2342, 3865
MmeI	TCCRAC	8 cut(s)	381, 690, 717, 956, 2269, 3024, 3187, 3444
MroI	TCCGGA	1 cut(s)	1651
MroNI	GCCGGC	1 cut(s)	1784
MroXI	GAANNNNTTC	3 cut(s)	492, 1542, 1847
MslI	CAYNNNNRTG	1 cut(s)	1282
MspA1I	CMGCKG	3 cut(s)	1921, 1958, 3732
MunI	CAATTG	3 cut(s)	1086, 1719, 2177
MvaI	CCWGG	6 cut(s)	896, 968, 974, 1513, 2550, 3548
MvnI	CGCG	7 cut(s)	559, 1155, 1174, 3732, 3935, 3937, 4118
NaeI	GCCGGC	1 cut(s)	1786
NciI	CCSGG	8 cut(s)	850, 955, 1429, 1570, 2264, 3184, 3185, 3263
NcoI	CCATGG	2 cut(s)	1041, 3751
NgoMIV	GCCGGC	1 cut(s)	1784
NlaIV	GGNNCC	9 cut(s)	355, 835, 1644, 2411, 2470, 3442, 3644, 3763, 4015
NmeAIII	GCCGAG	1 cut(s)	620
NmuCI	GTSAC	6 cut(s)	3, 146, 201, 598, 2345, 3991
NspI	RCATGY	4 cut(s)	16, 2288, 2461, 2713
NspV	TTCGAA	5 cut(s)	1841, 1910, 1937, 2057, 4124
PacI	TTAATTAA	1 cut(s)	4275
PaeI	GCATGC	1 cut(s)	2713
PaeR7I	CTCGAG	1 cut(s)	3983
PaqCI	CACCTGC	1 cut(s)	56
PasI	CCCWGGG	1 cut(s)	3547
PauI	GCGCGC	1 cut(s)	3933
PceI	AGGCCT	1 cut(s)	4192
PciI	ACATGT	1 cut(s)	2284
PcsI	WCGNNNNNNNCGW	3 cut(s)	459, 3816, 4130
PdiI	GCCGGC	1 cut(s)	1786
PdmI	GAANNNNTTC	3 cut(s)	492, 1542, 1847
PflMI	CCANNNNNTGG	1 cut(s)	2924
PfoI	TCCNGGA	2 cut(s)	966, 1568
PinAI	ACCGGT	1 cut(s)	2102
PleI	GAGTC	5 cut(s)	445, 605, 1332, 2342, 3865
PmaCI	CACGTG	1 cut(s)	206
PmlI	CACGTG	1 cut(s)	206
PpsI	GAGTC	5 cut(s)	445, 605, 1332, 2342, 3865
Ppu21I	YACGTR	2 cut(s)	206, 3022
PpuMI	RGGWCCY	1 cut(s)	2409
PscI	ACATGT	1 cut(s)	2284
PshBI	ATTAAT	2 cut(s)	237, 4230
PsiI	TTATAA	1 cut(s)	4260
Psp124BI	GAGCTC	3 cut(s)	609, 903, 1449
Psp1406I	AACGTT	1 cut(s)	3336
Psp5II	RGGWCCY	1 cut(s)	2409
Psp6I	CCWGG	6 cut(s)	894, 966, 972, 1511, 2548, 3546
PspCI	CACGTG	1 cut(s)	206
PspEI	GGTNACC	1 cut(s)	764
PspFI	CCCAGC	2 cut(s)	868, 4180
PspGI	CCWGG	6 cut(s)	894, 966, 972, 1511, 2548, 3546
PspN4I	GGNNCC	9 cut(s)	355, 835, 1644, 2411, 2470, 3442, 3644, 3763, 4015
PspPPI	RGGWCCY	1 cut(s)	2409
PspXI	VCTCGAGB	1 cut(s)	3983
PstI	CTGCAG	1 cut(s)	1372
PstNI	CAGNNNCTG	2 cut(s)	1321, 4069
PsuI	RGATCY	3 cut(s)	85, 2119, 3676
PteI	GCGCGC	1 cut(s)	3933
PvuII	CAGCTG	2 cut(s)	1921, 1958
RsaI	GTAC	8 cut(s)	197, 1271, 2193, 2542, 2619, 3449, 3590, 3883
RsaNI	GTAC	8 cut(s)	196, 1270, 2192, 2541, 2618, 3448, 3589, 3882
RseI	CAYNNNNRTG	1 cut(s)	1282
SacI	GAGCTC	3 cut(s)	609, 903, 1449
SacII	CCGCGG	1 cut(s)	3733
ScaI	AGTACT	1 cut(s)	3883
SchI	GAGTC	5 cut(s)	446, 606, 1333, 2342, 3865
SfaNI	GCATC	5 cut(s)	1395, 2925, 3339, 4056, 4293
SfcI	CTRYAG	3 cut(s)	1368, 3243, 3961
Sfr274I	CTCGAG	1 cut(s)	3983
Sfr303I	CCGCGG	1 cut(s)	3733
SfuI	TTCGAA	5 cut(s)	1841, 1910, 1937, 2057, 4124
SgrBI	CCGCGG	1 cut(s)	3733
SinI	GGWCC	7 cut(s)	546, 682, 970, 1111, 1643, 2409, 4013
SlaI	CTCGAG	1 cut(s)	3983
SmaI	CCCGGG	1 cut(s)	3185
SmiMI	CAYNNNNRTG	1 cut(s)	1282
SmlI	CTYRAG	7 cut(s)	1440, 1621, 2812, 2942, 3362, 3983, 4307
SmoI	CTYRAG	7 cut(s)	1440, 1621, 2812, 2942, 3362, 3983, 4307
SpeI	ACTAGT	1 cut(s)	3275
SphI	GCATGC	1 cut(s)	2713
SseBI	AGGCCT	1 cut(s)	4192
SspI	AATATT	2 cut(s)	2049, 4410
SspMI	CTAG	5 cut(s)	102, 1376, 2678, 3276, 3689
SstI	GAGCTC	3 cut(s)	609, 903, 1449
StuI	AGGCCT	1 cut(s)	4192
StyI	CCWWGG	6 cut(s)	280, 1041, 2405, 2902, 2923, 3751
TaaI	ACNGT	6 cut(s)	1083, 1385, 3117, 3445, 3478, 3593
TaqII	GACCGA	1 cut(s)	246
TatI	WGTACW	3 cut(s)	195, 3447, 3881
TauI	GCSGC	4 cut(s)	1177, 2145, 3358, 3735
TscAI	CASTG	5 cut(s)	84, 534, 766, 1467, 2470
TseFI	GTSAC	6 cut(s)	3, 146, 201, 598, 2345, 3991
Tsp45I	GTSAC	6 cut(s)	3, 146, 201, 598, 2345, 3991
TspDTI	ATGAA	8 cut(s)	379, 415, 2685, 2822, 2876, 3177, 3271, 4282
TspGWI	ACGGA	4 cut(s)	237, 1124, 1465, 2888
TspMI	CCCGGG	1 cut(s)	3183
TspRI	CASTG	5 cut(s)	84, 534, 766, 1467, 2470
Van91I	CCANNNNNTGG	1 cut(s)	2924
VneI	GTGCAC	1 cut(s)	2098
VpaK11BI	GGWCC	7 cut(s)	546, 682, 970, 1111, 1643, 2409, 4013
VspI	ATTAAT	2 cut(s)	237, 4230
XagI	CCTNNNNNAGG	1 cut(s)	2311
XceI	RCATGY	4 cut(s)	16, 2288, 2461, 2713
XhoI	CTCGAG	1 cut(s)	3983
XmaI	CCCGGG	1 cut(s)	3183
XmiI	GTMKAC	2 cut(s)	448, 3941
XmnI	GAANNNNTTC	3 cut(s)	492, 1542, 1847
XspI	CTAG	5 cut(s)	102, 1376, 2678, 3276, 3689
ZrmI	AGTACT	1 cut(s)	3883

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.