Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0008640

Physical Location & Seq

Genomic Coordinates Forward (+)

26352899 .. 26357263

Gene Structure 4365 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3189 bp

ATGGAGCCTCTTCCAGATGGTTCAAGGAATCCAAAGAGGAGTGCAATTAAGCAGGTTGCCAGTGGGAGATTTGGAGTTTCAAGTTACTATCTGACGAATGCTGATGAACTGCAGATAAAAATGGCTCAGGGTGCCAAGCCTGGTGAAGGAGGTGAGCTTCCTGGACACAAGGTTATTGGAGACATTGCTGTCACCAGGAATTCTACTGCAGGTGTGGGTCTTATCAGTCCACCTCCCCATCATGATATCTACTCGATTGAAGACCTTGCCCAATTAATTCATGATCTTAAGAACGCTAACCCAGCAGCTCGAATTAGTGTCAAGTTGGTATCGGAAGTAGGTGTGGGAGTAGTTGCTAGTGGAGTTGTGAAGGGGCATGCTGATCATGTATTGATCTCGGGACACGATGGAGGGACAGGGGCCTCTAGATGGACAGGAATTAAGAATGCAGGGCTTCCATGGGAACTTGGTTTGGCTGAGACTCATCAAACCTTGGTTTCTAATGATCTTCGTGGCCGCACAACTCTCCAGACTGATGGCCAACTAAAAACTGGAAGAGATGTTGCCATAGCTACACTTCTTGGTGCGGAAGAGTTTGGCTTCAGCACTGCACCTCTCATACCCCTTGGCTGCATTATGATGCGGAAGTGCCATAAGAACACATGTCCTGTTGGTATTGCAACTCAAGATCCAGTACTTCGTGAGAAGTTTGCTGGAGAACCTGAACATGTTATTAATTTTTTCTTTATGATAGCTGAGGAACTCAGAGAGATAATGGCACAGCTTGGTTTCCGTACTATAAATGAGATGGTTGGTCGTTCAGATATGCTTGAAGTTGATAGAGAAGTGACTAAAAACAATGAGAAGCTTGACAATATTGATCTATCTTTGTTACTTCGACCTGCAGCAGACCTTCGGCCTGGAGCTGCACAATATTGTGTTGAGAAACAAGACCATGGCTTGGATATGGCTCTTGATCACAAGCTAATTTCTATGTCTAAAGCTGCTTTGGAAAAAGCTCTTCCTGTATATTTTGAGACACCAATTTGCAATGTGAACCGTGCTGTTGGTACAATGCTTAGCCACGAGGTGACAAAACGTTACAACAGGGCTGGGCTTCCTGCTGACACCATTCATATCAAATTTAGTGGAAGTGGAGGACAGAGTCTTGGAGCATTTTTGTGCTCTGGAATCATGCTTGAGCTTGAGGGTGACAGTAATGATTATGTTGGTAAAGGTCTGTCTGGTGGAAAGATTGTAGTTTATCCTCCCAAGGGAAGCAGGTTTGACCCAAAAGAAAATATTGTCATCGGTAATGTAGCTTTATATGGTGCCACTAGTGGTGAGGCATACTTCAATGGAATGGCAGCAGAGAGATTTTGTGTTCGCAATTCAGGGGCTAAGGCAGTTGTCGAGGGTGTTGGTGATCATGGCTGTGAATACATGACAGGTGGCACTGTTGTTGTGCTCGGGAAAACTGGCAGGAACTTTGCTGCTGGTATGAGTGGTGGTATTGCCTATGTTTATGATGTGGATGGACAATTTCGGTCTCGATGTAATCCTGAGCTTGTCGATCTTGATACACTTGAAGAAGAAGATATTCTGACTCTTCAAATGATGATACAACAACATCAACGTCACACAAAAAGCCTGCTGGCCATTCAGGTGCTGGCTGATTTTGAAAATATTCTGCCTAAGTTTATCAAGGTTATCCCAAGAGAGTACAAGCGAGTTCTTGCAGATATGAAAGAGGAAACCAAACAGGTTATAGAGCACAAGGAAGATGAGCCAGAGCTTGAAGAGAAAGATGCTTTTGAAGAACTTAAGAAGTTGGCGGCTGCATCTTTAAATGGGAAATCCAATCAGGTAGAAGATGCTGAAGCATTGAAAAGGCCTTCTCAGGTCACTGATGCTATTAAACATCGTGGTTTTATTTATTATGAGCGCGAGGGCGTTCAATATAGGGATCGTAATGTTCGGATGAATGACTGGAAGGAAGTTATGGAGGAAACTAAACCTGGACCACTTGTGAAGACTCAGTCAGCCCGTTGCATGGACTGCGGGACTCCTTTCTGCCATCAGGAGAACTCTGGATGCCCTCTTGGTAACAAAATACCAGAGTTCAATGAGTTGGTGTACCAGAATAGGTGGCGTGAAGCATTAGAGAGACTTCTCGAGACAAATAACTTCCCTGAGTTTACTGGTCGTGTTTGTCCGGCACCATGTGAAGGTTCTTGTGTTCTGGGTATTATTGAGAATCCTGTATCTATCAAAAGCATAGAATGTGCCATCATAGACAAGGCTTTTGAGGAAGGTTGGATGGTGCCACGACCTCCCGTAAAGAGAACTGGGAAAAGGGTTGCCATTGTAGGAAGCGGGCCAGCTGGTCTAGCTGCTGCTGATCAGCTAAACAGAATAGGCCACACAGTGACTGTGTATGAGCGTGCTGATAGAATTGGAGGGCTTATGATGTATGGAGTGCCTAATATGAAGACTGATAAAGTGGAGATAGTTCAACGGCGGGTTAACCTTATGGCTGAGGAAGGTGTCAACTTTGTGGTTAATACTAATATTGGAAATGATCCTTTGTACTCACTTGATCGCCTTCGAGAGGAGAACAATGCCATTGTTTTAGCAGTAGGAGCAACAAAACCAAGGGACCTTCCTGTACCAGGGAGAGAGCTGTCAGGAGTACATTTTGCTATGGAATTTCTTCATGCAAACACCAAAAGCTTACTAGATAGCAATCTTGAGGATGGTAACTACATCTCTGCCAAGGGCAAGAAAGTTGTGGTAATTGGTGGAGGTGACACTGGCACTGATTGCATTGGAACATCCGTTCGCCATGGCTGCACTAACATCATAAATTTGGAGCTTCTTCCTGAGCCACCACGAAAAAGGGCTCCAGGAAACCCTTGGCCACAGTGGCCTCGTGTATTCCGTGTGGATTATGGGCACCAAGAAGTTGCAGCTAAGTTTGGCAAAGATCCAAGAACTTATGAGGTGCTGACAAAGAGATTTGTGGGAGATGAGAATGGGGCCGTGAAAGGACTCGAGGTGGTGCGTGTCAAATGGGAGAAGGATGAAACTGGGCGGTTCCAATTCAAGGAAATCGAGGGATCTGAGAGATCCTCGAGGCTGACCTCGTCCTACTCGCAATGGGGTTCCTTGGACCAGAGGCGACGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015930 GO:0016040 GO:0016053 GO:0016491 GO:0016638 GO:0016639 GO:0019676 GO:0019740 GO:0019752 GO:0032502 GO:0040007 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045181 GO:0046394 GO:0046686 GO:0048589 GO:0050896 GO:0055114 GO:0060359 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko00250 ko00910 ko01100 ko01110 ko01120 ko01130 ko01230 map00250 map00910 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

Fer4_20 GATase_2 GXGXG Glu_syn_central Glu_synthase Pyr_redox_2

Protein Analysis

1063

Amino Acids

116.71

Weight (kDa)

6.15

Isoelectric Point (pI)

35.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glu_synthase	PF01645	12 - 264	7.1e-117	Conserved region in glutamate synthase
GXGXG	PF01493	348 - 533	5.3e-88	GXGXG motif
Fer4_20	PF14691	661 - 772	2e-22	Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
Pyr_redox_2	PF07992	786 - 958	8.5e-16	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	789 - 824	8e-09	NAD(P)-binding Rossmann-like domain
Amino_oxidase	PF01593	795 - 823	4.1e-06	Flavin containing amine oxidoreductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53460 AT5G53460 AT5G53460
fragaria_vesca	FvH4_7g31770 FvH4_7g31770 FvH4_7g31770
malus_domestica	MD01G1219500.v1.1 MD07G1290500.v1.1
prunus_persica	Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1
pyrus_communis	pycom01g22930 pycom07g26320
rosa_chinensis	RchiOBHm_Chr1g0381001
rosa_laevigata	RLG00000026253
rosa_multiflora	Rmu_ssc0000032.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278870
rosa_rugosa	Rorug01G0426400
rosa_samantha	Rh1AG450100 Rh1BG406100 Rh1CG420100 Rh1DG436600
rosa_wichuraiana	Rw1G039220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	200
AasI	GACNNNNNNGTC	1 cut(s)	188
AbsI	CCTCGAGG	1 cut(s)	3133
Acc36I	ACCTGC	4 cut(s)	43, 200, 910, 1272
AccB1I	GGYRCC	5 cut(s)	131, 1331, 2218, 2323, 2955
AccB7I	CCANNNNNTGG	1 cut(s)	961
AccII	CGCG	1 cut(s)	1947
AciI	CCGC	8 cut(s)	517, 587, 643, 1835, 2061, 2376, 2521, 3094
AclI	AACGTT	1 cut(s)	1099
AclWI	GGATC	6 cut(s)	683, 1974, 2576, 2981, 3123, 3127
AcoI	YGGCCR	4 cut(s)	514, 538, 1656, 2918
AcsI	RAATTY	4 cut(s)	199, 1142, 2708, 2866
AcuI	CTGAAG	2 cut(s)	586, 1899
AdeI	CACNNNGTG	3 cut(s)	1090, 2225, 2428
AfaI	GTAC	8 cut(s)	696, 796, 1072, 1724, 2138, 2591, 2670, 2694
AfiI	CCNNNNNNNGG	9 cut(s)	146, 429, 961, 1274, 2053, 2228, 2611, 2672, 3106
AflII	CTTAAG	2 cut(s)	287, 1823
AflIII	ACRYGT	2 cut(s)	662, 727
AhlI	ACTAGT	1 cut(s)	1337
AjnI	CCWGG	7 cut(s)	139, 160, 194, 919, 2017, 2671, 2905
AloI	GAACNNNNNNTCC	2 cut(s)	2823, 2855
Alw21I	GWGCWC	3 cut(s)	1187, 1470, 1776
Alw26I	GTCTC	6 cut(s)	174, 473, 1031, 1554, 2161, 2171
AlwI	GGATC	6 cut(s)	683, 1974, 2576, 2981, 3123, 3127
AlwNI	CAGNNNCTG	2 cut(s)	1669, 2432
Ama87I	CYCGRG	5 cut(s)	397, 1469, 2174, 3053, 3133
ApoI	RAATTY	4 cut(s)	199, 1142, 2708, 2866
ArsI	GACNNNNNNTTYG	2 cut(s)	2288, 2320
AseI	ATTAAT	2 cut(s)	275, 735
Asp700I	GAANNNNTTC	3 cut(s)	1599, 1686, 2712
AspLEI	GCGC	1 cut(s)	1947
AspS9I	GGNCC	6 cut(s)	420, 2021, 2378, 2659, 3039, 3172
AsuHPI	GGTGA	8 cut(s)	155, 164, 184, 1102, 1223, 1355, 1436, 2819
AvaI	CYCGRG	5 cut(s)	397, 1469, 2174, 3053, 3133
AvaII	GGWCC	3 cut(s)	2021, 2659, 3172
BaeGI	GKGCMC	1 cut(s)	2958
BalI	TGGCCA	3 cut(s)	540, 1658, 2920
BanI	GGYRCC	5 cut(s)	131, 1331, 2218, 2323, 2955
BanII	GRGCYC	1 cut(s)	2905
BauI	CACGAG	2 cut(s)	1085, 2931
BbsI	GAAGAC	3 cut(s)	267, 2039, 2498
Bbv12I	GWGCWC	3 cut(s)	1187, 1470, 1776
BbvCI	CCTCAGC	2 cut(s)	756, 2538
BceAI	ACGGC	2 cut(s)	2534, 3026
BciT130I	CCWGG	7 cut(s)	141, 162, 196, 921, 2019, 2673, 2907
BclI	TGATCA	4 cut(s)	382, 976, 1426, 2401
BcoDI	GTCTC	6 cut(s)	174, 473, 1031, 1554, 2161, 2171
BcuI	ACTAGT	1 cut(s)	1337
BfaI	CTAG	5 cut(s)	357, 426, 1338, 2390, 2738
BfmI	CTRYAG	3 cut(s)	110, 207, 903
BfrI	CTTAAG	2 cut(s)	287, 1823
BfuAI	ACCTGC	4 cut(s)	43, 200, 910, 1272
BglI	GCCNNNNNGGC	1 cut(s)	2926
BlpI	GCTNAGC	1 cut(s)	1079
BmcAI	AGTACT	1 cut(s)	696
Bme1390I	CCNGG	7 cut(s)	141, 162, 196, 921, 2019, 2673, 2907
Bme18I	GGWCC	3 cut(s)	2021, 2659, 3172
BmeT110I	CYCGRG	5 cut(s)	397, 1469, 2174, 3053, 3133
BmgT120I	GGNCC	6 cut(s)	420, 2021, 2378, 2659, 3039, 3172
BmrFI	CCNGG	7 cut(s)	141, 162, 196, 921, 2019, 2673, 2907
BmrI	ACTGGG	2 cut(s)	2358, 3099
BmsI	GCATC	6 cut(s)	630, 1798, 1850, 1864, 1900, 2084
BmuI	ACTGGG	2 cut(s)	2358, 3099
BpiI	GAAGAC	3 cut(s)	267, 2039, 2498
BplI	GAGNNNNNCTC	2 cut(s)	3116, 3148
BpmI	CTGGAG	4 cut(s)	512, 735, 942, 2889
Bpu10I	CCTNAGC	6 cut(s)	126, 756, 1401, 1563, 2538, 2883
Bpu1102I	GCTNAGC	1 cut(s)	1079
BpuEI	CTTGAG	4 cut(s)	669, 1220, 1226, 2771
BsaBI	GATNNNNATC	1 cut(s)	2745
BsaI	GGTCTC	1 cut(s)	1554
Bsc4I	CCNNNNNNNGG	9 cut(s)	146, 429, 961, 1274, 2053, 2228, 2611, 2672, 3106
Bse1I	ACTGG	9 cut(s)	60, 556, 692, 1483, 1994, 2206, 2353, 2818, 3094
Bse3DI	GCAATG	3 cut(s)	183, 1057, 3164
Bse8I	GATNNNNATC	1 cut(s)	2745
BseBI	CCWGG	7 cut(s)	141, 162, 196, 921, 2019, 2673, 2907
BseGI	GGATG	7 cut(s)	1540, 1986, 2099, 2325, 2761, 2834, 3088
BseJI	GATNNNNATC	1 cut(s)	2745
BseLI	CCNNNNNNNGG	9 cut(s)	146, 429, 961, 1274, 2053, 2228, 2611, 2672, 3106
BseMI	GCAATG	3 cut(s)	183, 1057, 3164
BseNI	ACTGG	9 cut(s)	60, 556, 692, 1483, 1994, 2206, 2353, 2818, 3094
BseRI	GAGGAG	2 cut(s)	52, 2627
BseSI	GKGCMC	1 cut(s)	2958
BseYI	CCCAGC	2 cut(s)	301, 1112
BsgI	GTGCAG	3 cut(s)	594, 912, 2836
Bsh1236I	CGCG	1 cut(s)	1947
BshNI	GGYRCC	5 cut(s)	131, 1331, 2218, 2323, 2955
BsiHKAI	GWGCWC	3 cut(s)	1187, 1470, 1776
BsiHKCI	CYCGRG	5 cut(s)	397, 1469, 2174, 3053, 3133
BsiSI	CCGG	1 cut(s)	2216
BslFI	GGGAC	4 cut(s)	414, 427, 2077, 2672
BslI	CCNNNNNNNGG	9 cut(s)	146, 429, 961, 1274, 2053, 2228, 2611, 2672, 3106
BsmAI	GTCTC	6 cut(s)	174, 473, 1031, 1554, 2161, 2171
BsmFI	GGGAC	4 cut(s)	414, 427, 2077, 2672
BsmI	GAATGC	2 cut(s)	103, 451
Bso31I	GGTCTC	1 cut(s)	1554
BsoBI	CYCGRG	5 cut(s)	397, 1469, 2174, 3053, 3133
Bsp1286I	GDGCHC	5 cut(s)	1187, 1470, 1776, 2905, 2958
Bsp1720I	GCTNAGC	1 cut(s)	1079
Bsp19I	CCATGG	3 cut(s)	458, 955, 2845
BspACI	CCGC	8 cut(s)	517, 587, 643, 1835, 2061, 2376, 2521, 3094
BspFNI	CGCG	1 cut(s)	1947
BspHI	TCATGA	2 cut(s)	241, 280
BspMAI	CTGCAG	3 cut(s)	114, 211, 907
BspMI	ACCTGC	4 cut(s)	43, 200, 910, 1272
BspPI	GGATC	6 cut(s)	683, 1974, 2576, 2981, 3123, 3127
BspQI	GCTCTTC	1 cut(s)	1026
BspT107I	GGYRCC	5 cut(s)	131, 1331, 2218, 2323, 2955
BspTI	CTTAAG	2 cut(s)	287, 1823
BspTNI	GGTCTC	1 cut(s)	1554
BsrDI	GCAATG	3 cut(s)	183, 1057, 3164
BsrI	ACTGG	9 cut(s)	60, 556, 692, 1483, 1994, 2206, 2353, 2818, 3094
BssSI	CACGAG	2 cut(s)	1085, 2931
Bst2BI	CACGAG	2 cut(s)	1085, 2931
Bst2UI	CCWGG	7 cut(s)	141, 162, 196, 921, 2019, 2673, 2907
Bst4CI	ACNGT	7 cut(s)	1061, 1217, 1459, 2428, 2434, 2925, 3186
Bst6I	CTCTTC	6 cut(s)	15, 550, 585, 1026, 1614, 1794
BstAFI	CTTAAG	2 cut(s)	287, 1823
BstAPI	GCANNNNNTGC	2 cut(s)	2058, 2823
BstC8I	GCNNGC	7 cut(s)	378, 1652, 1656, 1671, 2378, 2382, 2445
BstDSI	CCRYGG	3 cut(s)	458, 955, 2845
BstENI	CCTNNNNNAGG	3 cut(s)	144, 2609, 2670
BstF5I	GGATG	7 cut(s)	1540, 1986, 2099, 2325, 2761, 2834, 3088
BstFNI	CGCG	1 cut(s)	1947
BstHHI	GCGC	1 cut(s)	1947
BstMAI	GTCTC	6 cut(s)	174, 473, 1031, 1554, 2161, 2171
BstMWI	GCNNNNNNNGC	8 cut(s)	131, 302, 2058, 2390, 2642, 2823, 2850, 2926
BstNI	CCWGG	7 cut(s)	141, 162, 196, 921, 2019, 2673, 2907
BstNSI	RCATGY	3 cut(s)	380, 666, 731
BstSCI	CCNGG	7 cut(s)	139, 160, 194, 919, 2017, 2671, 2905
BstSFI	CTRYAG	3 cut(s)	110, 207, 903
BstSLI	GKGCMC	1 cut(s)	2958
BstUI	CGCG	1 cut(s)	1947
BstV2I	GAAGAC	3 cut(s)	267, 2039, 2498
BstX2I	RGATCY	4 cut(s)	688, 2986, 3119, 3128
BstXI	CCANNNNNNTGG	1 cut(s)	536
BstYI	RGATCY	4 cut(s)	688, 2986, 3119, 3128
BtgI	CCRYGG	3 cut(s)	458, 955, 2845
BtsCI	GGATG	7 cut(s)	1540, 1986, 2099, 2325, 2761, 2834, 3088
BtsI	GCAGTG	1 cut(s)	606
BtsIMutI	CAGTG	8 cut(s)	67, 606, 1455, 1905, 2433, 2811, 2817, 2930
BveI	ACCTGC	4 cut(s)	43, 200, 910, 1272
Cac8I	GCNNGC	7 cut(s)	378, 1652, 1656, 1671, 2378, 2382, 2445
CaiI	CAGNNNCTG	2 cut(s)	1669, 2432
CciI	TCATGA	2 cut(s)	241, 280
CfoI	GCGC	1 cut(s)	1947
Cfr13I	GGNCC	6 cut(s)	420, 2021, 2378, 2659, 3039, 3172
Csp6I	GTAC	8 cut(s)	695, 795, 1071, 1723, 2137, 2590, 2669, 2693
CspCI	CAANNNNNGTGG	2 cut(s)	1129, 1164
CviQI	GTAC	8 cut(s)	695, 795, 1071, 1723, 2137, 2590, 2669, 2693
DraI	TTTAAA	1 cut(s)	1848
DraIII	CACNNNGTG	3 cut(s)	1090, 2225, 2428
DrdI	GACNNNNNNGTC	1 cut(s)	188
DseDI	GACNNNNNNGTC	1 cut(s)	188
EaeI	YGGCCR	4 cut(s)	514, 538, 1656, 2918
Eam1104I	CTCTTC	6 cut(s)	15, 550, 585, 1026, 1614, 1794
EarI	CTCTTC	6 cut(s)	15, 550, 585, 1026, 1614, 1794
Eco147I	AGGCCT	1 cut(s)	1894
Eco24I	GRGCYC	1 cut(s)	2905
Eco31I	GGTCTC	1 cut(s)	1554
Eco32I	GATATC	1 cut(s)	247
Eco47I	GGWCC	3 cut(s)	2021, 2659, 3172
Eco57I	CTGAAG	2 cut(s)	586, 1899
Eco88I	CYCGRG	5 cut(s)	397, 1469, 2174, 3053, 3133
EcoNI	CCTNNNNNAGG	3 cut(s)	144, 2609, 2670
EcoO109I	RGGNCCY	2 cut(s)	420, 2659
EcoRI	GAATTC	1 cut(s)	199
EcoRII	CCWGG	7 cut(s)	139, 160, 194, 919, 2017, 2671, 2905
EcoRV	GATATC	1 cut(s)	247
EcoT38I	GRGCYC	1 cut(s)	2905
FaqI	GGGAC	4 cut(s)	414, 427, 2077, 2672
FauI	CCCGC	3 cut(s)	2054, 2369, 2514
FbaI	TGATCA	4 cut(s)	382, 976, 1426, 2401
FokI	GGATG	7 cut(s)	1547, 1993, 2106, 2332, 2768, 2821, 3095
FriOI	GRGCYC	1 cut(s)	2905
FspBI	CTAG	5 cut(s)	357, 426, 1338, 2390, 2738
GlaI	GCGC	1 cut(s)	1946
GsaI	CCCAGC	2 cut(s)	305, 1116
GsuI	CTGGAG	4 cut(s)	512, 735, 942, 2889
HapII	CCGG	1 cut(s)	2216
HhaI	GCGC	1 cut(s)	1947
Hin6I	GCGC	1 cut(s)	1945
HinP1I	GCGC	1 cut(s)	1945
HincII	GTYRAC	2 cut(s)	2527, 2551
HindII	GTYRAC	2 cut(s)	2527, 2551
HindIII	AAGCTT	2 cut(s)	866, 2731
HinfI	GANTC	9 cut(s)	28, 481, 1165, 1191, 1606, 2035, 2065, 2257, 3051
HpaI	GTTAAC	1 cut(s)	2527
HpaII	CCGG	1 cut(s)	2216
HphI	GGTGA	8 cut(s)	155, 164, 184, 1102, 1223, 1355, 1436, 2819
Hpy166II	GTNNAC	6 cut(s)	230, 1057, 2137, 2199, 2527, 2551
Hpy188I	TCNGA	7 cut(s)	93, 334, 767, 823, 1605, 1980, 3124
Hpy8I	GTNNAC	6 cut(s)	230, 1057, 2137, 2199, 2527, 2551
Hpy99I	CGWCG	1 cut(s)	3186
HpyCH4III	ACNGT	7 cut(s)	1061, 1217, 1459, 2428, 2434, 2925, 3186
HpyCH4IV	ACGT	2 cut(s)	1099, 1636
HpyF10VI	GCNNNNNNNGC	8 cut(s)	131, 302, 2058, 2390, 2642, 2823, 2850, 2926
HpySE526I	ACGT	2 cut(s)	1099, 1636
HspAI	GCGC	1 cut(s)	1945
Ksp22I	TGATCA	4 cut(s)	382, 976, 1426, 2401
KspAI	GTTAAC	1 cut(s)	2527
LguI	GCTCTTC	1 cut(s)	1026
LmnI	GCTCC	6 cut(s)	4, 923, 1172, 2642, 2872, 2908
LweI	GCATC	6 cut(s)	630, 1798, 1850, 1864, 1900, 2084
MaeI	CTAG	5 cut(s)	357, 426, 1338, 2390, 2738
MaeII	ACGT	2 cut(s)	1099, 1636
MflI	RGATCY	4 cut(s)	688, 2986, 3119, 3128
MhlI	GDGCHC	5 cut(s)	1187, 1470, 1776, 2905, 2958
MlsI	TGGCCA	3 cut(s)	540, 1658, 2920
MluNI	TGGCCA	3 cut(s)	540, 1658, 2920
MlyI	GAGTC	6 cut(s)	475, 1174, 1600, 2029, 2059, 3045
MmeI	TCCRAC	1 cut(s)	2297
Mox20I	TGGCCA	3 cut(s)	540, 1658, 2920
MroXI	GAANNNNTTC	3 cut(s)	1599, 1686, 2712
MscI	TGGCCA	3 cut(s)	540, 1658, 2920
MslI	CAYNNNNRTG	4 cut(s)	638, 1180, 1434, 1664
Msp20I	TGGCCA	3 cut(s)	540, 1658, 2920
MspA1I	CMGCKG	1 cut(s)	2384
MspCI	CTTAAG	2 cut(s)	287, 1823
MspI	CCGG	1 cut(s)	2216
MspR9I	CCNGG	7 cut(s)	141, 162, 196, 921, 2019, 2673, 2907
Mva1269I	GAATGC	2 cut(s)	103, 451
MvaI	CCWGG	7 cut(s)	141, 162, 196, 921, 2019, 2673, 2907
MvnI	CGCG	1 cut(s)	1947
MwoI	GCNNNNNNNGC	8 cut(s)	131, 302, 2058, 2390, 2642, 2823, 2850, 2926
NcoI	CCATGG	3 cut(s)	458, 955, 2845
NmuCI	GTSAC	8 cut(s)	190, 847, 1090, 1211, 1637, 1903, 2428, 2807
NspI	RCATGY	3 cut(s)	380, 666, 731
PaeI	GCATGC	1 cut(s)	380
PaeR7I	CTCGAG	3 cut(s)	2174, 3053, 3133
PagI	TCATGA	2 cut(s)	241, 280
PaqCI	CACCTGC	1 cut(s)	200
PceI	AGGCCT	1 cut(s)	1894
PciI	ACATGT	2 cut(s)	662, 727
PciSI	GCTCTTC	1 cut(s)	1026
PcsI	WCGNNNNNNNCGW	1 cut(s)	2938
PctI	GAATGC	2 cut(s)	103, 451
PdmI	GAANNNNTTC	3 cut(s)	1599, 1686, 2712
PfeI	GAWTC	3 cut(s)	28, 1191, 2257
PflFI	GACNNNGTC	3 cut(s)	1164, 2038, 3145
PflMI	CCANNNNNTGG	1 cut(s)	961
PfoI	TCCNGGA	2 cut(s)	160, 2905
PleI	GAGTC	6 cut(s)	475, 1173, 1600, 2029, 2059, 3045
PpsI	GAGTC	6 cut(s)	475, 1173, 1600, 2029, 2059, 3045
PpuMI	RGGWCCY	1 cut(s)	2659
PscI	ACATGT	2 cut(s)	662, 727
PshBI	ATTAAT	2 cut(s)	275, 735
Psp1406I	AACGTT	1 cut(s)	1099
Psp5II	RGGWCCY	1 cut(s)	2659
Psp6I	CCWGG	7 cut(s)	139, 160, 194, 919, 2017, 2671, 2905
PspFI	CCCAGC	2 cut(s)	301, 1112
PspGI	CCWGG	7 cut(s)	139, 160, 194, 919, 2017, 2671, 2905
PspPI	GGNCC	6 cut(s)	420, 2021, 2378, 2659, 3039, 3172
PspPPI	RGGWCCY	1 cut(s)	2659
PspXI	VCTCGAGB	2 cut(s)	3053, 3133
PstI	CTGCAG	3 cut(s)	114, 211, 907
PstNI	CAGNNNCTG	2 cut(s)	1669, 2432
PsuI	RGATCY	4 cut(s)	688, 2986, 3119, 3128
PsyI	GACNNNGTC	3 cut(s)	1164, 2038, 3145
PvuII	CAGCTG	1 cut(s)	2384
RsaI	GTAC	8 cut(s)	696, 796, 1072, 1724, 2138, 2591, 2670, 2694
RsaNI	GTAC	8 cut(s)	695, 795, 1071, 1723, 2137, 2590, 2669, 2693
RseI	CAYNNNNRTG	4 cut(s)	638, 1180, 1434, 1664
SapI	GCTCTTC	1 cut(s)	1026
Sau96I	GGNCC	6 cut(s)	420, 2021, 2378, 2659, 3039, 3172
ScaI	AGTACT	1 cut(s)	696
SchI	GAGTC	6 cut(s)	475, 1174, 1600, 2029, 2059, 3045
ScrFI	CCNGG	7 cut(s)	141, 162, 196, 921, 2019, 2673, 2907
SduI	GDGCHC	5 cut(s)	1187, 1470, 1776, 2905, 2958
SfaNI	GCATC	6 cut(s)	630, 1798, 1850, 1864, 1900, 2084
SfcI	CTRYAG	3 cut(s)	110, 207, 903
SfiI	GGCCNNNNNGGCC	1 cut(s)	2926
Sfr274I	CTCGAG	3 cut(s)	2174, 3053, 3133
SinI	GGWCC	3 cut(s)	2021, 2659, 3172
SlaI	CTCGAG	3 cut(s)	2174, 3053, 3133
SmiMI	CAYNNNNRTG	4 cut(s)	638, 1180, 1434, 1664
SmlI	CTYRAG	9 cut(s)	287, 684, 1199, 1205, 1823, 2174, 2750, 3053, 3133
SmoI	CTYRAG	9 cut(s)	287, 684, 1199, 1205, 1823, 2174, 2750, 3053, 3133
SpeI	ACTAGT	1 cut(s)	1337
SphI	GCATGC	1 cut(s)	380
SseBI	AGGCCT	1 cut(s)	1894
SsiI	CCGC	8 cut(s)	517, 587, 643, 1835, 2061, 2376, 2521, 3094
SspI	AATATT	5 cut(s)	877, 935, 1303, 1687, 2572
SspMI	CTAG	5 cut(s)	357, 426, 1338, 2390, 2738
StuI	AGGCCT	1 cut(s)	1894
StyD4I	CCNGG	7 cut(s)	139, 160, 194, 919, 2017, 2671, 2905
TaaI	ACNGT	7 cut(s)	1061, 1217, 1459, 2428, 2434, 2925, 3186
TaiI	ACGT	2 cut(s)	1102, 1639
TaqII	GACCGA	1 cut(s)	1536
TatI	WGTACW	4 cut(s)	694, 1722, 2589, 2692
TauI	GCSGC	2 cut(s)	519, 1838
TfiI	GAWTC	3 cut(s)	28, 1191, 2257
TscAI	CASTG	8 cut(s)	67, 613, 1462, 1912, 2433, 2818, 2824, 2930
TseFI	GTSAC	8 cut(s)	190, 847, 1090, 1211, 1637, 1903, 2428, 2807
Tsp45I	GTSAC	8 cut(s)	190, 847, 1090, 1211, 1637, 1903, 2428, 2807
TspDTI	ATGAA	8 cut(s)	120, 269, 1124, 1760, 1997, 2504, 2705, 3099
TspGWI	ACGGA	3 cut(s)	782, 2827, 2930
TspRI	CASTG	8 cut(s)	67, 613, 1462, 1912, 2433, 2818, 2824, 2930
Tth111I	GACNNNGTC	3 cut(s)	1164, 2038, 3145
Van91I	CCANNNNNTGG	1 cut(s)	961
Vha464I	CTTAAG	2 cut(s)	287, 1823
VpaK11BI	GGWCC	3 cut(s)	2021, 2659, 3172
VspI	ATTAAT	2 cut(s)	275, 735
XagI	CCTNNNNNAGG	3 cut(s)	144, 2609, 2670
XapI	RAATTY	4 cut(s)	199, 1142, 2708, 2866
XbaI	TCTAGA	1 cut(s)	425
XceI	RCATGY	3 cut(s)	380, 666, 731
XcmI	CCANNNNNNNNNTGG	3 cut(s)	548, 1666, 2913
XhoI	CTCGAG	3 cut(s)	2174, 3053, 3133
XmnI	GAANNNNTTC	3 cut(s)	1599, 1686, 2712
XspI	CTAG	5 cut(s)	357, 426, 1338, 2390, 2738
ZrmI	AGTACT	1 cut(s)	696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.