pycom07g27510

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006698
Physical Location & Seq
Reverse (-)
27215531 .. 27218055
2525 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1344 bp
ATGACGAAGACGAAGGGAAGGATTTCGAGAATTGGAAGCTATGCAATTGCGTCTTCCAAACTAGACGGCCACCACCAACAGCCATGCATTACCTGCACCACCTTCAACATCCTCGCTCCAATTTACAAGCGCCTCAACCATGACGATCCTGATAGTCGAGAAAGCGATCACAGAGCGTATTGGCTGGCCAGGAACCAAAGGATACTGGATTGTTTGTTGGCTGAGAGATCTTCCATCATTTGTCTTCAGGAGTTCTGGGTCGGAAACGAAGAGCTTGTGAACATTTACGAGAAGAGACTTGGAGATGCTGGTTATATCAATCTCAAACTAGGACGAACCAACAACCGTGGCGATGGTTTGCTGACCGCGGTGCATAAGGACTACTTTAGAGTTGTAAACTATGAAGAGTTGCACTTCAATGATTATGGTGATCGTGTTGCTCAGTTGATACATGTGGAATTAGCTACCCCAATTTTGCAGTTCCGAAACAATGACATTCGGCAAGAAATTCTCATTGTGAACACCCACTTGTTATTTCCTCATGACTCGAGTTTGTGTATAGTTCGATTGCATCAGGTCTACAAAATCTTACAACATTTAGAATCTTATCAGAAAGAGAACAAGCTTAATCCCATGCCGATTATACTCTGCGGTGACTGGAATGGAAGCAAACGTGGACATGTTTACGAGTTTCTTCGGTCCCAGGGGTTTGTATCTTCTTATGATACTGCTCATCATTATACCGATGCAGATGCACACAAGTGGGTTAGCCACAGAAACCATAGGGGAAACATATGCGGCGTTGATTTTATATGGCTTCTCAATCCTAATAGATATCGTAAGCTACTAAAAACTAGTTGGACTGAAGCAGTATTTGGAATGTTCAAGTATCTACTACGAAGAGCTTCGCTGACAGCAGATGATGCCTTTGCTTTTCTAAAGGCTGATAATCCTGTTGATTACATTACCTATTCAGGTTTCTGTGAAGCACTTCGACAGCTTAACATGACAGGTCATTGCCATGGACTTAGCCACGACGAGGCAAAGGATTTGTGGGTTCAAGCAGACATAGATGGAAATGGTTTTCTTGATTACATAGAATTTCAGCAGCGAATTTGGAATCCTACGGGGTCAGAGCAGAAAGATGAAAGCGGTAGTGACTGTGATAGTGATTCCAAGGGTATTCAGGAACAAACAATTGGTTTCAGTGTAAAAAACGCGGTTCTCTTCCCTCCTGAAGTGGAGAAAGGAACGTGGCCCGAAGACTACTCTCTTTCCGATCATGCACGACTCACTGTGGTTTTTTCACCAATAAGAATGCCTTGCTCTCAGATGATACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

448

Amino Acids

51.52

Weight (kDa)

6.31

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 34 - 320 1.9e-09 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 101
AccI GTMKAC 1 cut(s) 579
AccII CGCG 2 cut(s) 368, 1220
AciI CCGC 6 cut(s) 366, 368, 651, 798, 1152, 1220
AclWI GGATC 1 cut(s) 140
AcoI YGGCCR 2 cut(s) 67, 186
AcsI RAATTY 3 cut(s) 507, 1100, 1113
AcuI CTGAAG 3 cut(s) 230, 885, 1257
AfiI CCNNNNNNNGG 1 cut(s) 1039
AflIII ACRYGT 2 cut(s) 451, 679
AgsI TTSAA 4 cut(s) 106, 418, 886, 1061
AhlI ACTAGT 1 cut(s) 854
AjnI CCWGG 2 cut(s) 188, 702
AjuI GAANNNNNNNTTGG 4 cut(s) 858, 890, 1182, 1214
AleI CACNNNNGTG 1 cut(s) 760
AluBI AGCT 7 cut(s) 39, 274, 464, 625, 844, 905, 1000
AluI AGCT 7 cut(s) 39, 274, 464, 625, 844, 905, 1000
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 547
AoxI GGCC 3 cut(s) 67, 186, 1256
ApeKI GCWGC 1 cut(s) 1108
ApoI RAATTY 3 cut(s) 507, 1100, 1113
Asp700I GAANNNNTTC 3 cut(s) 22, 904, 990
AspLEI GCGC 1 cut(s) 132
AspS9I GGNCC 2 cut(s) 699, 1257
AsuHPI GGTGA 3 cut(s) 440, 665, 1299
AvaI CYCGRG 1 cut(s) 547
AvaII GGWCC 1 cut(s) 699
BalI TGGCCA 1 cut(s) 188
BbsI GAAGAC 4 cut(s) 14, 45, 236, 1269
BbvI GCAGC 1 cut(s) 1120
BccI CCATC 3 cut(s) 242, 347, 1067
BceAI ACGGC 1 cut(s) 82
BcgI CGANNNNNNTGC 2 cut(s) 734, 768
BciT130I CCWGG 2 cut(s) 190, 704
BciVI GTATCC 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 289
BcuI ACTAGT 1 cut(s) 854
BfaI CTAG 3 cut(s) 62, 329, 855
BfoI RGCGCY 1 cut(s) 133
BfuAI ACCTGC 1 cut(s) 101
BfuI GTATCC 1 cut(s) 195
BglII AGATCT 1 cut(s) 227
BisI GCNGC 2 cut(s) 799, 1109
BlsI GCNGC 2 cut(s) 800, 1110
Bme1390I CCNGG 2 cut(s) 190, 704
Bme18I GGWCC 1 cut(s) 699
BmeT110I CYCGRG 1 cut(s) 547
BmgT120I GGNCC 2 cut(s) 699, 1257
BmiI GGNNCC 2 cut(s) 194, 701
BmrFI CCNGG 2 cut(s) 190, 704
BmsI GCATC 5 cut(s) 295, 580, 736, 742, 913
BpiI GAAGAC 4 cut(s) 14, 45, 236, 1269
BsaJI CCNNGG 6 cut(s) 346, 366, 702, 703, 1021, 1176
Bsc4I CCNNNNNNNGG 1 cut(s) 1039
Bse1I ACTGG 2 cut(s) 210, 662
Bse3DI GCAATG 1 cut(s) 1015
BseBI CCWGG 2 cut(s) 190, 704
BseDI CCNNGG 6 cut(s) 346, 366, 702, 703, 1021, 1176
BseGI GGATG 1 cut(s) 108
BseLI CCNNNNNNNGG 1 cut(s) 1039
BseMI GCAATG 1 cut(s) 1015
BseMII CTCAG 3 cut(s) 213, 455, 1343
BseNI ACTGG 2 cut(s) 210, 662
BseXI GCAGC 1 cut(s) 1120
BsgI GTGCAG 1 cut(s) 79
Bsh1236I CGCG 2 cut(s) 368, 1220
BshFI GGCC 3 cut(s) 69, 188, 1258
BsiHKCI CYCGRG 1 cut(s) 547
BslFI GGGAC 1 cut(s) 685
BslI CCNNNNNNNGG 1 cut(s) 1039
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 685
BsmI GAATGC 1 cut(s) 1323
BsnI GGCC 3 cut(s) 69, 188, 1258
BsoBI CYCGRG 1 cut(s) 547
Bsp143I GATC 5 cut(s) 145, 166, 227, 430, 1279
Bsp19I CCATGG 1 cut(s) 1021
BspACI CCGC 6 cut(s) 366, 368, 651, 798, 1152, 1220
BspANI GGCC 3 cut(s) 69, 188, 1258
BspCNI CTCAG 3 cut(s) 214, 454, 1342
BspFNI CGCG 2 cut(s) 368, 1220
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 2 cut(s) 194, 701
BspMI ACCTGC 1 cut(s) 101
BspPI GGATC 1 cut(s) 140
BspQI GCTCTTC 2 cut(s) 264, 895
BsrDI GCAATG 1 cut(s) 1015
BsrI ACTGG 2 cut(s) 210, 662
BssECI CCNNGG 6 cut(s) 346, 366, 702, 703, 1021, 1176
BssMI GATC 5 cut(s) 145, 166, 227, 430, 1279
BssT1I CCWWGG 2 cut(s) 1021, 1176
Bst2UI CCWGG 2 cut(s) 190, 704
Bst4CI ACNGT 3 cut(s) 347, 1163, 1297
Bst6I CTCTTC 5 cut(s) 264, 287, 399, 895, 1232
BstAPI GCANNNNNTGC 2 cut(s) 93, 923
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 4 cut(s) 222, 441, 1028, 1329
BstDSI CCRYGG 3 cut(s) 346, 366, 1021
BstF5I GGATG 1 cut(s) 108
BstFNI CGCG 2 cut(s) 368, 1220
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 1 cut(s) 132
BstKTI GATC 5 cut(s) 148, 169, 230, 433, 1282
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 5 cut(s) 145, 166, 227, 430, 1279
BstMWI GCNNNNNNNGC 2 cut(s) 93, 923
BstNI CCWGG 2 cut(s) 190, 704
BstNSI RCATGY 2 cut(s) 455, 683
BstSCI CCNGG 2 cut(s) 188, 702
BstUI CGCG 2 cut(s) 368, 1220
BstV1I GCAGC 1 cut(s) 1120
BstV2I GAAGAC 4 cut(s) 14, 45, 236, 1269
BstX2I RGATCY 1 cut(s) 227
BstYI RGATCY 1 cut(s) 227
BsuI GTATCC 1 cut(s) 195
BsuRI GGCC 3 cut(s) 69, 188, 1258
BtgI CCRYGG 3 cut(s) 346, 366, 1021
BtgZI GCGATG 1 cut(s) 366
BtsCI GGATG 1 cut(s) 108
BtsIMutI CAGTG 2 cut(s) 1213, 1293
BveI ACCTGC 1 cut(s) 101
Cac8I GCNNGC 1 cut(s) 186
CciI TCATGA 1 cut(s) 541
CfoI GCGC 1 cut(s) 132
Cfr13I GGNCC 2 cut(s) 699, 1257
Cfr42I CCGCGG 1 cut(s) 369
CseI GACGC 1 cut(s) 39
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 9 cut(s) 84, 140, 452, 542, 634, 680, 1006, 1022, 1283
DdeI CTNAG 4 cut(s) 222, 441, 1028, 1329
DpnI GATC 5 cut(s) 147, 168, 229, 432, 1281
DpnII GATC 5 cut(s) 145, 166, 227, 430, 1279
EaeI YGGCCR 2 cut(s) 67, 186
Eam1104I CTCTTC 5 cut(s) 264, 287, 399, 895, 1232
EarI CTCTTC 5 cut(s) 264, 287, 399, 895, 1232
Eco130I CCWWGG 2 cut(s) 1021, 1176
Eco32I GATATC 1 cut(s) 836
Eco47I GGWCC 1 cut(s) 699
Eco57I CTGAAG 3 cut(s) 230, 885, 1257
Eco88I CYCGRG 1 cut(s) 547
EcoRII CCWGG 2 cut(s) 188, 702
EcoRV GATATC 1 cut(s) 836
EcoT14I CCWWGG 2 cut(s) 1021, 1176
EcoT22I ATGCAT 1 cut(s) 89
ErhI CCWWGG 2 cut(s) 1021, 1176
FaeI CATG 9 cut(s) 87, 143, 455, 545, 637, 683, 1009, 1025, 1286
FalI AAGNNNNNCTT 4 cut(s) 368, 400, 1306, 1338
FaqI GGGAC 1 cut(s) 685
FatI CATG 9 cut(s) 83, 139, 451, 541, 633, 679, 1005, 1021, 1282
FauNDI CATATG 1 cut(s) 794
FblI GTMKAC 1 cut(s) 579
Fnu4HI GCNGC 2 cut(s) 799, 1109
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 2 cut(s) 799, 1109
FspBI CTAG 3 cut(s) 62, 329, 855
GlaI GCGC 1 cut(s) 131
GluI GCNGC 2 cut(s) 799, 1109
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 3 cut(s) 69, 188, 1258
HgaI GACGC 1 cut(s) 39
HhaI GCGC 1 cut(s) 132
Hin1II CATG 9 cut(s) 87, 143, 455, 545, 637, 683, 1009, 1025, 1286
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 5 cut(s) 545, 602, 1120, 1172, 1290
HphI GGTGA 3 cut(s) 440, 665, 1299
Hpy166II GTNNAC 6 cut(s) 280, 397, 520, 580, 677, 685
Hpy188I TCNGA 7 cut(s) 263, 485, 612, 1135, 1279, 1332, 1343
Hpy188III TCNNGA 8 cut(s) 27, 149, 158, 248, 542, 1088, 1187, 1235
Hpy8I GTNNAC 6 cut(s) 280, 397, 520, 580, 677, 685
Hpy99I CGWCG 1 cut(s) 1040
HpyAV CCTTC 3 cut(s) 7, 12, 112
HpyCH4III ACNGT 3 cut(s) 347, 1163, 1297
HpyCH4IV ACGT 2 cut(s) 673, 1253
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 923
HpyF3I CTNAG 4 cut(s) 222, 441, 1028, 1329
HpySE526I ACGT 2 cut(s) 673, 1253
Hsp92II CATG 9 cut(s) 87, 143, 455, 545, 637, 683, 1009, 1025, 1286
HspAI GCGC 1 cut(s) 130
KspI CCGCGG 1 cut(s) 369
Kzo9I GATC 5 cut(s) 145, 166, 227, 430, 1279
LguI GCTCTTC 2 cut(s) 264, 895
LmnI GCTCC 1 cut(s) 121
Lsp1109I GCAGC 1 cut(s) 1120
LweI GCATC 5 cut(s) 295, 580, 736, 742, 913
MaeI CTAG 3 cut(s) 62, 329, 855
MaeII ACGT 2 cut(s) 673, 1253
MaeIII GTNAC 2 cut(s) 653, 1157
MalI GATC 5 cut(s) 147, 168, 229, 432, 1281
MboI GATC 5 cut(s) 145, 166, 227, 430, 1279
MfeI CAATTG 2 cut(s) 45, 1197
MflI RGATCY 1 cut(s) 227
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 9 cut(s) 30, 45, 120, 458, 471, 507, 1100, 1113, 1197
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 2 cut(s) 539, 1284
MmeI TCCRAC 2 cut(s) 241, 839
MnlI CCTC 5 cut(s) 122, 143, 549, 1033, 1242
Mox20I TGGCCA 1 cut(s) 188
Mph1103I ATGCAT 1 cut(s) 89
MroXI GAANNNNTTC 3 cut(s) 22, 904, 990
MscI TGGCCA 1 cut(s) 188
MseI TTAA 2 cut(s) 627, 1002
MslI CAYNNNNRTG 3 cut(s) 417, 760, 1020
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 368
MspR9I CCNGG 2 cut(s) 190, 704
MunI CAATTG 2 cut(s) 45, 1197
Mva1269I GAATGC 1 cut(s) 1323
MvaI CCWGG 2 cut(s) 190, 704
MvnI CGCG 2 cut(s) 368, 1220
MwoI GCNNNNNNNGC 2 cut(s) 93, 923
NcoI CCATGG 1 cut(s) 1021
NdeI CATATG 1 cut(s) 794
NdeII GATC 5 cut(s) 145, 166, 227, 430, 1279
NlaIII CATG 9 cut(s) 87, 143, 455, 545, 637, 683, 1009, 1025, 1286
NlaIV GGNNCC 2 cut(s) 194, 701
NmuCI GTSAC 2 cut(s) 653, 1157
NsiI ATGCAT 1 cut(s) 89
NspI RCATGY 2 cut(s) 455, 683
OliI CACNNNNGTG 1 cut(s) 760
PaeR7I CTCGAG 1 cut(s) 547
PagI TCATGA 1 cut(s) 541
PasI CCCWGGG 1 cut(s) 703
PciI ACATGT 2 cut(s) 451, 679
PciSI GCTCTTC 2 cut(s) 264, 895
PctI GAATGC 1 cut(s) 1323
PdmI GAANNNNTTC 3 cut(s) 22, 904, 990
PfeI GAWTC 3 cut(s) 602, 1120, 1172
PkrI GCNGC 2 cut(s) 800, 1110
PleI GAGTC 2 cut(s) 539, 1284
PpsI GAGTC 2 cut(s) 539, 1284
PscI ACATGT 2 cut(s) 451, 679
Psp6I CCWGG 2 cut(s) 188, 702
PspGI CCWGG 2 cut(s) 188, 702
PspN4I GGNNCC 2 cut(s) 194, 701
PspPI GGNCC 2 cut(s) 699, 1257
PspXI VCTCGAGB 1 cut(s) 547
PsuI RGATCY 1 cut(s) 227
RseI CAYNNNNRTG 3 cut(s) 417, 760, 1020
SacII CCGCGG 1 cut(s) 369
SapI GCTCTTC 2 cut(s) 264, 895
SaqAI TTAA 2 cut(s) 627, 1002
SatI GCNGC 2 cut(s) 799, 1109
Sau3AI GATC 5 cut(s) 145, 166, 227, 430, 1279
Sau96I GGNCC 2 cut(s) 699, 1257
SchI GAGTC 2 cut(s) 539, 1284
ScrFI CCNGG 2 cut(s) 190, 704
SfaNI GCATC 5 cut(s) 295, 580, 736, 742, 913
Sfr274I CTCGAG 1 cut(s) 547
Sfr303I CCGCGG 1 cut(s) 369
SgrBI CCGCGG 1 cut(s) 369
SinI GGWCC 1 cut(s) 699
SlaI CTCGAG 1 cut(s) 547
SmiMI CAYNNNNRTG 3 cut(s) 417, 760, 1020
SmlI CTYRAG 1 cut(s) 547
SmoI CTYRAG 1 cut(s) 547
SpeI ACTAGT 1 cut(s) 854
Sse9I AATT 9 cut(s) 30, 45, 120, 458, 471, 507, 1100, 1113, 1197
SsiI CCGC 6 cut(s) 366, 368, 651, 798, 1152, 1220
SspMI CTAG 3 cut(s) 62, 329, 855
StyD4I CCNGG 2 cut(s) 188, 702
StyI CCWWGG 2 cut(s) 1021, 1176
TaaI ACNGT 3 cut(s) 347, 1163, 1297
TaiI ACGT 2 cut(s) 676, 1256
TaqI TCGA 5 cut(s) 26, 157, 548, 565, 994
TaqII GACCGA 1 cut(s) 687
TasI AATT 9 cut(s) 30, 45, 120, 458, 471, 507, 1100, 1113, 1197
TauI GCSGC 1 cut(s) 801
TfiI GAWTC 3 cut(s) 602, 1120, 1172
Tru1I TTAA 2 cut(s) 627, 1002
Tru9I TTAA 2 cut(s) 627, 1002
TscAI CASTG 2 cut(s) 1213, 1300
TseFI GTSAC 2 cut(s) 653, 1157
TseI GCWGC 1 cut(s) 1108
Tsp45I GTSAC 2 cut(s) 653, 1157
TspDTI ATGAA 2 cut(s) 417, 1161
TspRI CASTG 2 cut(s) 1213, 1300
VpaK11BI GGWCC 1 cut(s) 699
XapI RAATTY 3 cut(s) 507, 1100, 1113
XceI RCATGY 2 cut(s) 455, 683
XhoI CTCGAG 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 579
XmnI GAANNNNTTC 3 cut(s) 22, 904, 990
XspI CTAG 3 cut(s) 62, 329, 855
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.