pycom08g00170

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0016192
Physical Location & Seq
Reverse (-)
156539 .. 157555
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 906 bp
ATGTCAAGGGTGAAGCCGGATCGCAAGCCTCCGCTGGCTAAATCACCAATTAGGCTTCGACCCCGTCGGGTCCTCGGATCAAACTCAAACGTTATGCAAACCCCATCCGGACGGTCTGAGATCCGGTTCCAGCAGCAAGCCGATCCAATTTTAACAGGAGGGTCTTTGAGAAGATCGGAGAAAGCAAGGGGCACAAGCGGCACATGGGAGATGGAAGAAGCGGATCTTCGACCCGAATACCAGTCGATTTCGTGGGAACTGAGGGCTTTAGCCAAGATGGTTGGTGACGAATTGGGCAAGGAAAATTGTGATGCTGCTGATATGGATAGTGATATGAAGATGAATTGTGGTGGTGGGAATTCGTTGGTTTCGAGCCCTTTGTTCGAAAGGGGTAGATTTTACGAGCAGTACTCTGCCAGAAGGAACGAGCGGCTCAATAGGAAGAACAAGATCAAAGAGGCTCAAACAAATTCTTTTTCGTCAACAACCGCGTCTGTGTCTAAATTTAAAAGCTATAATAATAAGCTGGGTGTTACGGTAGAGTCTGCCAAAAGAAGCAGTTTTACCAGTACTACCAGTAGCAGCAGCAGGAAGCTTCAAAGCAGCTTGAGGAAATCGGTGGTCTCTGCCGTGGTGGAGACTCCAATTCCAACTCCAACTCCAACTCCAACTCCAATTCAGCGGAAGAGCACGAGTAGTCAGTGCGAGAAACAGGCTCCTCCGACTCTAATTCCAACTCCAACTCCGAGGTACATGCTGAGGAGCATGACGAACAAAGAGAATATGAAGCTGCCTCCTCTGCCTCAGCAGCCTTCTCATAATAATCCTAAGGCATCTGCCATCCGTGGGGGACGCAAAAGCGGAGCTCCATGTTCAACTCCATCAAGAGTCGTTGGGGGGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

302

Amino Acids

33.18

Weight (kDa)

10.36

Isoelectric Point (pI)

59.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016192)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42330
fragaria_vesca FvH4_2g37430
prunus_persica Prupe.1G354300_v2.0.a1
pyrus_communis pycom08g00170
rosa_chinensis RchiOBHm_Chr6g0304291
rosa_laevigata RLG00000010994
rosa_multiflora Rmu_sc0001652.1_g000001 Rmu_ssc0000218.1_g000035
rosa_roxburghii Rroxscaffold_7G00163870
rosa_rugosa Rorug06G0329700
rosa_samantha Rh6AG443100 Rh6BG459300 Rh6CG455900 Rh6DG442600
rosa_wichuraiana Rw6G038450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 430
AccII CGCG 1 cut(s) 491
AccIII TCCGGA 1 cut(s) 107
AciI CCGC 7 cut(s) 32, 198, 221, 430, 489, 682, 861
AclI AACGTT 1 cut(s) 90
AclWI GGATC 5 cut(s) 27, 85, 115, 137, 231
AcsI RAATTY 3 cut(s) 358, 469, 503
AfaI GTAC 3 cut(s) 410, 571, 752
AfiI CCNNNNNNNGG 1 cut(s) 846
AgsI TTSAA 2 cut(s) 599, 876
AluBI AGCT 6 cut(s) 513, 526, 595, 606, 790, 866
AluI AGCT 6 cut(s) 513, 526, 595, 606, 790, 866
Alw21I GWGCWC 2 cut(s) 692, 868
Alw26I GTCTC 2 cut(s) 628, 632
AlwI GGATC 5 cut(s) 27, 85, 115, 137, 231
Aor13HI TCCGGA 1 cut(s) 107
ApeKI GCWGC 7 cut(s) 133, 314, 582, 585, 603, 790, 808
ApoI RAATTY 3 cut(s) 358, 469, 503
AspS9I GGNCC 1 cut(s) 70
AsuHPI GGTGA 3 cut(s) 22, 36, 296
AsuII TTCGAA 1 cut(s) 384
AvaII GGWCC 1 cut(s) 70
AxyI CCTNAGG 1 cut(s) 828
BaeGI GKGCMC 1 cut(s) 194
BanII GRGCYC 2 cut(s) 377, 868
BarI GAAGNNNNNNTAC 2 cut(s) 547, 579
BauI CACGAG 1 cut(s) 691
Bbv12I GWGCWC 2 cut(s) 692, 868
BbvCI CCTCAGC 2 cut(s) 758, 804
BbvI GCAGC 7 cut(s) 145, 301, 594, 597, 615, 777, 820
BccI CCATC 5 cut(s) 112, 205, 271, 848, 889
BceAI ACGGC 1 cut(s) 614
BcgI CGANNNNNNTGC 2 cut(s) 736, 770
BcoDI GTCTC 2 cut(s) 628, 632
BisI GCNGC 9 cut(s) 134, 199, 315, 431, 583, 586, 604, 791, 809
BlsI GCNGC 9 cut(s) 135, 200, 316, 432, 584, 587, 605, 792, 810
BmcAI AGTACT 2 cut(s) 410, 571
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 3 cut(s) 71, 128, 717
BmsI GCATC 2 cut(s) 301, 842
BplI GAGNNNNNCTC 2 cut(s) 395, 427
Bpu10I CCTNAGC 2 cut(s) 758, 804
Bpu14I TTCGAA 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 628
BsaI GGTCTC 1 cut(s) 628
BsaJI CCNNGG 4 cut(s) 73, 630, 746, 844
BsaWI WCCGGW 2 cut(s) 107, 123
BsaXI ACNNNNNCTCC 8 cut(s) 643, 649, 655, 673, 679, 685, 727, 757
Bsc4I CCNNNNNNNGG 1 cut(s) 846
Bse1I ACTGG 3 cut(s) 241, 567, 576
Bse21I CCTNAGG 1 cut(s) 828
BseAI TCCGGA 1 cut(s) 107
BseDI CCNNGG 4 cut(s) 73, 630, 746, 844
BseGI GGATG 2 cut(s) 104, 840
BseLI CCNNNNNNNGG 1 cut(s) 846
BseMII CTCAG 4 cut(s) 108, 251, 749, 818
BseNI ACTGG 3 cut(s) 241, 567, 576
BseRI GAGGAG 3 cut(s) 708, 775, 786
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 7 cut(s) 145, 301, 594, 597, 615, 777, 820
BseYI CCCAGC 1 cut(s) 526
Bsh1236I CGCG 1 cut(s) 491
BsiHKAI GWGCWC 2 cut(s) 692, 868
BsiSI CCGG 3 cut(s) 17, 108, 124
BslFI GGGAC 1 cut(s) 864
BslI CCNNNNNNNGG 1 cut(s) 846
BsmAI GTCTC 2 cut(s) 628, 632
BsmFI GGGAC 1 cut(s) 864
Bso31I GGTCTC 1 cut(s) 628
Bsp119I TTCGAA 1 cut(s) 384
Bsp1286I GDGCHC 4 cut(s) 194, 377, 692, 868
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 7 cut(s) 19, 77, 120, 142, 173, 223, 450
BspACI CCGC 7 cut(s) 32, 198, 221, 430, 489, 682, 861
BspCNI CTCAG 4 cut(s) 109, 252, 750, 817
BspEI TCCGGA 1 cut(s) 107
BspFNI CGCG 1 cut(s) 491
BspLI GGNNCC 3 cut(s) 71, 128, 717
BspPI GGATC 5 cut(s) 27, 85, 115, 137, 231
BspQI GCTCTTC 1 cut(s) 680
BspT104I TTCGAA 1 cut(s) 384
BspTNI GGTCTC 1 cut(s) 628
BsrBI CCGCTC 1 cut(s) 430
BsrI ACTGG 3 cut(s) 241, 567, 576
BssECI CCNNGG 4 cut(s) 73, 630, 746, 844
BssMI GATC 7 cut(s) 19, 77, 120, 142, 173, 223, 450
BssSI CACGAG 1 cut(s) 691
Bst2BI CACGAG 1 cut(s) 691
Bst4CI ACNGT 2 cut(s) 114, 538
Bst6I CTCTTC 1 cut(s) 680
BstBI TTCGAA 1 cut(s) 384
BstC8I GCNNGC 3 cut(s) 26, 36, 138
BstDEI CTNAG 5 cut(s) 117, 260, 758, 804, 828
BstDSI CCRYGG 2 cut(s) 630, 844
BstF5I GGATG 2 cut(s) 104, 840
BstFNI CGCG 1 cut(s) 491
BstKTI GATC 7 cut(s) 22, 80, 123, 145, 176, 226, 453
BstMAI GTCTC 2 cut(s) 628, 632
BstMBI GATC 7 cut(s) 19, 77, 120, 142, 173, 223, 450
BstMWI GCNNNNNNNGC 3 cut(s) 198, 799, 808
BstNSI RCATGY 1 cut(s) 757
BstSLI GKGCMC 1 cut(s) 194
BstUI CGCG 1 cut(s) 491
BstV1I GCAGC 7 cut(s) 145, 301, 594, 597, 615, 777, 820
BstX2I RGATCY 2 cut(s) 120, 223
BstYI RGATCY 2 cut(s) 120, 223
Bsu36I CCTNAGG 1 cut(s) 828
BtgI CCRYGG 2 cut(s) 630, 844
BtsCI GGATG 2 cut(s) 104, 840
BtsIMutI CAGTG 1 cut(s) 707
Cac8I GCNNGC 3 cut(s) 26, 36, 138
Cfr13I GGNCC 1 cut(s) 70
CseI GACGC 2 cut(s) 480, 861
Csp6I GTAC 3 cut(s) 409, 570, 751
CviAII CATG 4 cut(s) 204, 754, 766, 870
CviQI GTAC 3 cut(s) 409, 570, 751
DdeI CTNAG 5 cut(s) 117, 260, 758, 804, 828
DpnI GATC 7 cut(s) 21, 79, 122, 144, 175, 225, 452
DpnII GATC 7 cut(s) 19, 77, 120, 142, 173, 223, 450
DraI TTTAAA 1 cut(s) 508
Eam1104I CTCTTC 1 cut(s) 680
EarI CTCTTC 1 cut(s) 680
Ecl136II GAGCTC 1 cut(s) 866
Eco24I GRGCYC 2 cut(s) 377, 868
Eco31I GGTCTC 1 cut(s) 628
Eco47I GGWCC 1 cut(s) 70
Eco53kI GAGCTC 1 cut(s) 866
Eco81I CCTNAGG 1 cut(s) 828
EcoICRI GAGCTC 1 cut(s) 866
EcoO109I RGGNCCY 1 cut(s) 70
EcoRI GAATTC 1 cut(s) 358
EcoT38I GRGCYC 2 cut(s) 377, 868
FaeI CATG 4 cut(s) 207, 757, 769, 873
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 1 cut(s) 864
FatI CATG 4 cut(s) 203, 753, 765, 869
Fnu4HI GCNGC 9 cut(s) 134, 199, 315, 431, 583, 586, 604, 791, 809
FokI GGATG 2 cut(s) 91, 827
FriOI GRGCYC 2 cut(s) 377, 868
Fsp4HI GCNGC 9 cut(s) 134, 199, 315, 431, 583, 586, 604, 791, 809
GluI GCNGC 9 cut(s) 134, 199, 315, 431, 583, 586, 604, 791, 809
GsaI CCCAGC 1 cut(s) 530
HapII CCGG 3 cut(s) 17, 108, 124
HgaI GACGC 2 cut(s) 480, 861
Hin1II CATG 4 cut(s) 207, 757, 769, 873
HincII GTYRAC 1 cut(s) 483
HindII GTYRAC 1 cut(s) 483
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 4 cut(s) 542, 640, 724, 888
HpaII CCGG 3 cut(s) 17, 108, 124
HphI GGTGA 3 cut(s) 22, 36, 296
Hpy166II GTNNAC 1 cut(s) 483
Hpy188I TCNGA 5 cut(s) 77, 118, 178, 723, 747
Hpy188III TCNNGA 2 cut(s) 108, 885
Hpy8I GTNNAC 1 cut(s) 483
Hpy99I CGWCG 1 cut(s) 69
HpyAV CCTTC 2 cut(s) 414, 822
HpyCH4III ACNGT 2 cut(s) 114, 538
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 1 cut(s) 97
HpyF10VI GCNNNNNNNGC 3 cut(s) 198, 799, 808
HpyF3I CTNAG 5 cut(s) 117, 260, 758, 804, 828
HpySE526I ACGT 1 cut(s) 90
Hsp92II CATG 4 cut(s) 207, 757, 769, 873
Kpn2I TCCGGA 1 cut(s) 107
Kzo9I GATC 7 cut(s) 19, 77, 120, 142, 173, 223, 450
LguI GCTCTTC 1 cut(s) 680
LmnI GCTCC 4 cut(s) 721, 762, 863, 871
Lsp1109I GCAGC 7 cut(s) 145, 301, 594, 597, 615, 777, 820
LweI GCATC 2 cut(s) 301, 842
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 284, 532
MalI GATC 7 cut(s) 21, 79, 122, 144, 175, 225, 452
MbiI CCGCTC 1 cut(s) 430
MboI GATC 7 cut(s) 19, 77, 120, 142, 173, 223, 450
MboII GAAGA 6 cut(s) 183, 218, 227, 349, 454, 697
MflI RGATCY 2 cut(s) 120, 223
MhlI GDGCHC 4 cut(s) 194, 377, 692, 868
MlyI GAGTC 4 cut(s) 551, 634, 718, 897
MmeI TCCRAC 7 cut(s) 674, 680, 686, 692, 746, 758, 764
MroI TCCGGA 1 cut(s) 107
MseI TTAA 3 cut(s) 152, 507, 904
MspA1I CMGCKG 2 cut(s) 34, 682
MspI CCGG 3 cut(s) 17, 108, 124
MvnI CGCG 1 cut(s) 491
MwoI GCNNNNNNNGC 3 cut(s) 198, 799, 808
NdeII GATC 7 cut(s) 19, 77, 120, 142, 173, 223, 450
NlaIII CATG 4 cut(s) 207, 757, 769, 873
NlaIV GGNNCC 3 cut(s) 71, 128, 717
NmuCI GTSAC 1 cut(s) 284
NspI RCATGY 1 cut(s) 757
NspV TTCGAA 1 cut(s) 384
PciSI GCTCTTC 1 cut(s) 680
PcsI WCGNNNNNNNCGW 1 cut(s) 368
PflFI GACNNNGTC 1 cut(s) 63
PkrI GCNGC 9 cut(s) 135, 200, 316, 432, 584, 587, 605, 792, 810
PleI GAGTC 4 cut(s) 550, 634, 718, 896
PpsI GAGTC 4 cut(s) 550, 634, 718, 896
PpuMI RGGWCCY 1 cut(s) 70
Psp124BI GAGCTC 1 cut(s) 868
Psp1406I AACGTT 1 cut(s) 90
Psp5II RGGWCCY 1 cut(s) 70
PspFI CCCAGC 1 cut(s) 526
PspN4I GGNNCC 3 cut(s) 71, 128, 717
PspPI GGNCC 1 cut(s) 70
PspPPI RGGWCCY 1 cut(s) 70
PsuI RGATCY 2 cut(s) 120, 223
PsyI GACNNNGTC 1 cut(s) 63
RsaI GTAC 3 cut(s) 410, 571, 752
RsaNI GTAC 3 cut(s) 409, 570, 751
SacI GAGCTC 1 cut(s) 868
SapI GCTCTTC 1 cut(s) 680
SaqAI TTAA 3 cut(s) 152, 507, 904
SatI GCNGC 9 cut(s) 134, 199, 315, 431, 583, 586, 604, 791, 809
Sau3AI GATC 7 cut(s) 19, 77, 120, 142, 173, 223, 450
Sau96I GGNCC 1 cut(s) 70
ScaI AGTACT 2 cut(s) 410, 571
SchI GAGTC 4 cut(s) 551, 634, 718, 897
SduI GDGCHC 4 cut(s) 194, 377, 692, 868
SetI ASST 8 cut(s) 93, 515, 528, 597, 608, 752, 792, 868
SfaNI GCATC 2 cut(s) 301, 842
SfuI TTCGAA 1 cut(s) 384
SinI GGWCC 1 cut(s) 70
SmlI CTYRAG 1 cut(s) 607
SmoI CTYRAG 1 cut(s) 607
SsiI CCGC 7 cut(s) 32, 198, 221, 430, 489, 682, 861
SstI GAGCTC 1 cut(s) 868
TaaI ACNGT 2 cut(s) 114, 538
TaiI ACGT 1 cut(s) 93
TaqI TCGA 5 cut(s) 58, 229, 245, 371, 384
TatI WGTACW 2 cut(s) 408, 569
TauI GCSGC 2 cut(s) 201, 433
Tru1I TTAA 3 cut(s) 152, 507, 904
Tru9I TTAA 3 cut(s) 152, 507, 904
TscAI CASTG 1 cut(s) 707
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 7 cut(s) 133, 314, 582, 585, 603, 790, 808
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 3 cut(s) 350, 356, 800
TspGWI ACGGA 1 cut(s) 833
TspRI CASTG 1 cut(s) 707
Tth111I GACNNNGTC 1 cut(s) 63
VpaK11BI GGWCC 1 cut(s) 70
XapI RAATTY 3 cut(s) 358, 469, 503
XceI RCATGY 1 cut(s) 757
ZrmI AGTACT 2 cut(s) 410, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.