pycom08g00670

Nucleoredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009764
Physical Location & Seq
Reverse (-)
534117 .. 538235
4119 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00670.2

Sequence Viewer

Length: 762 bp
ATGAAGGAGATGAACGTGAAGATCAGTCATGGTTCTGAAGCTGCAGTAAGCAACGGTGGTGGTGGTGGGTATGGTGGCGCTGCTGTCTCGAGCTCAAGAGTTTCATCTCTCCTGGCCTCCAAAGATCGTGACTACCTTCTTTCTCCGACTGGAAACCAGGTGACAGTTTCTGATCTTAATGAGAAGATAATAGGCATCTACTTCTCAGCCAATTGGTACCCGCCATGCTGGAACTTCAACCAAGTCCTGGTTGGCATCTACGAGCAGCTGAAGAGCAGTGGGTCCAACTTTGAGGTTGTGTACGTGTCATCTGACGAAGACTCTGAAGCCTTTAACAAATACCATGCATGCATGCCGTGGCTGGCGATTCCTTTCTCTGACTTGGACACCAAGAAAGCCTTGAACCGTAAGTTTGATGTGGAAAGTATTCCCAGCTTGGTTATTTTGCAACCTAATGATAACAAGGAAGAGGCGTTGAACGATGGAGTTGAAATTATCTACCGTTACGGGGTCGATGCCTTCCCTTTTACAAAACAAAGGTTGGAGGAATTGCAAGATGAGGAGAAGGCGAGGCATGAGAACCAAACTCTGACCAATTTACTAACAAACCATGATAGAGATTATCTTTTGGGTCACCCCACGCCTAAACAGGTGTCAGTTGCTTCATTGAGAGGCAAAACCATTGGACTCTACTTCTCAGCTCAATGGTGTCGTCCGTGCGTTAATTTCACTCCAAGGTTGATCGATCCATCAACGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 Thioredoxin_8

Protein Analysis

254

Amino Acids

28.2

Weight (kDa)

5.29

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 216
AccB1I GGYRCC 1 cut(s) 216
AccB7I CCANNNNNTGG 1 cut(s) 247
AciI CCGC 1 cut(s) 221
AclWI GGATC 1 cut(s) 740
AcuI CTGAAG 3 cut(s) 57, 290, 345
AfaI GTAC 2 cut(s) 218, 302
AfiI CCNNNNNNNGG 2 cut(s) 247, 508
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 4 cut(s) 238, 403, 478, 491
AjnI CCWGG 3 cut(s) 111, 156, 246
AluBI AGCT 5 cut(s) 41, 93, 268, 435, 701
AluI AGCT 5 cut(s) 41, 93, 268, 435, 701
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 1 cut(s) 91
AlwI GGATC 1 cut(s) 740
AlwNI CAGNNNCTG 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 88
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 3 cut(s) 41, 80, 265
Asp700I GAANNNNTTC 1 cut(s) 426
Asp718I GGTACC 1 cut(s) 216
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 282
AsuHPI GGTGA 2 cut(s) 172, 626
AvaI CYCGRG 1 cut(s) 88
AvaII GGWCC 1 cut(s) 282
BanI GGYRCC 1 cut(s) 216
BanII GRGCYC 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 3 cut(s) 28, 67, 277
BccI CCATC 2 cut(s) 476, 757
BceAI ACGGC 1 cut(s) 340
BciT130I CCWGG 3 cut(s) 113, 158, 248
BcoDI GTCTC 1 cut(s) 91
BfmI CTRYAG 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 81
BisI GCNGC 3 cut(s) 42, 81, 266
BlsI GCNGC 3 cut(s) 43, 82, 267
Bme1390I CCNGG 3 cut(s) 113, 158, 248
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 2 cut(s) 218, 283
BmrFI CCNGG 3 cut(s) 113, 158, 248
BmsI GCATC 3 cut(s) 204, 264, 505
BpiI GAAGAC 1 cut(s) 324
BpuEI CTTGAG 1 cut(s) 79
Bsa29I ATCGAT 1 cut(s) 744
BsaAI YACGTR 1 cut(s) 304
BsaJI CCNNGG 2 cut(s) 356, 734
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 508
Bse1I ACTGG 1 cut(s) 154
BseBI CCWGG 3 cut(s) 113, 158, 248
BseCI ATCGAT 1 cut(s) 744
BseDI CCNNGG 2 cut(s) 356, 734
BseLI CCNNNNNNNGG 2 cut(s) 247, 508
BseMII CTCAG 2 cut(s) 219, 711
BseNI ACTGG 1 cut(s) 154
BseRI GAGGAG 1 cut(s) 575
BseXI GCAGC 3 cut(s) 28, 67, 277
BseYI CCCAGC 1 cut(s) 431
BshFI GGCC 1 cut(s) 116
BshNI GGYRCC 1 cut(s) 216
BshVI ATCGAT 1 cut(s) 744
BsiHKAI GWGCWC 1 cut(s) 95
BsiHKCI CYCGRG 1 cut(s) 88
BslI CCNNNNNNNGG 2 cut(s) 247, 508
BsmAI GTCTC 1 cut(s) 91
BsnI GGCC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 88
Bsp1286I GDGCHC 1 cut(s) 95
Bsp143I GATC 5 cut(s) 21, 124, 172, 741, 745
BspACI CCGC 1 cut(s) 221
BspANI GGCC 1 cut(s) 116
BspCNI CTCAG 2 cut(s) 218, 710
BspDI ATCGAT 1 cut(s) 744
BspLI GGNNCC 2 cut(s) 218, 283
BspMAI CTGCAG 1 cut(s) 46
BspPI GGATC 1 cut(s) 740
BspQI GCTCTTC 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 216
BsrI ACTGG 1 cut(s) 154
BssECI CCNNGG 2 cut(s) 356, 734
BssMI GATC 5 cut(s) 21, 124, 172, 741, 745
BssT1I CCWWGG 1 cut(s) 734
Bst2UI CCWGG 3 cut(s) 113, 158, 248
Bst4CI ACNGT 4 cut(s) 56, 166, 407, 503
Bst6I CTCTTC 2 cut(s) 266, 462
BstBAI YACGTR 1 cut(s) 304
BstC8I GCNNGC 3 cut(s) 349, 353, 363
BstDEI CTNAG 2 cut(s) 205, 697
BstDSI CCRYGG 1 cut(s) 356
BstEII GGTNACC 1 cut(s) 632
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 5 cut(s) 24, 127, 175, 744, 748
BstMAI GTCTC 1 cut(s) 91
BstMBI GATC 5 cut(s) 21, 124, 172, 741, 745
BstNI CCWGG 3 cut(s) 113, 158, 248
BstNSI RCATGY 2 cut(s) 351, 355
BstPI GGTNACC 1 cut(s) 632
BstSCI CCNGG 3 cut(s) 111, 156, 246
BstSFI CTRYAG 1 cut(s) 42
BstV1I GCAGC 3 cut(s) 28, 67, 277
BstV2I GAAGAC 1 cut(s) 324
Bsu15I ATCGAT 1 cut(s) 744
BsuRI GGCC 1 cut(s) 116
BsuTUI ATCGAT 1 cut(s) 744
BtgI CCRYGG 1 cut(s) 356
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 1 cut(s) 283
Cac8I GCNNGC 3 cut(s) 349, 353, 363
CaiI CAGNNNCTG 1 cut(s) 170
CfoI GCGC 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 282
ClaI ATCGAT 1 cut(s) 744
CsiI ACCWGGT 1 cut(s) 156
Csp6I GTAC 2 cut(s) 217, 301
CspCI CAANNNNNGTGG 2 cut(s) 40, 75
CviAII CATG 8 cut(s) 29, 225, 344, 348, 352, 575, 611, 759
CviQI GTAC 2 cut(s) 217, 301
DdeI CTNAG 2 cut(s) 205, 697
DpnI GATC 5 cut(s) 23, 126, 174, 743, 747
DpnII GATC 5 cut(s) 21, 124, 172, 741, 745
Eam1104I CTCTTC 2 cut(s) 266, 462
EarI CTCTTC 2 cut(s) 266, 462
Ecl136II GAGCTC 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 734
Eco24I GRGCYC 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 93
Eco57I CTGAAG 3 cut(s) 57, 290, 345
Eco88I CYCGRG 1 cut(s) 88
Eco91I GGTNACC 1 cut(s) 632
EcoICRI GAGCTC 1 cut(s) 93
EcoO65I GGTNACC 1 cut(s) 632
EcoRII CCWGG 3 cut(s) 111, 156, 246
EcoT14I CCWWGG 1 cut(s) 734
EcoT22I ATGCAT 2 cut(s) 349, 353
EcoT38I GRGCYC 1 cut(s) 95
ErhI CCWWGG 1 cut(s) 734
FaeI CATG 8 cut(s) 32, 228, 347, 351, 355, 578, 614, 762
FaiI YATR 9 cut(s) 30, 72, 226, 345, 349, 353, 576, 612, 760
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FatI CATG 8 cut(s) 28, 224, 343, 347, 351, 574, 610, 758
FauI CCCGC 1 cut(s) 228
Fnu4HI GCNGC 3 cut(s) 42, 81, 266
FriOI GRGCYC 1 cut(s) 95
Fsp4HI GCNGC 3 cut(s) 42, 81, 266
GlaI GCGC 1 cut(s) 79
GluI GCNGC 3 cut(s) 42, 81, 266
GsaI CCCAGC 1 cut(s) 435
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 1 cut(s) 116
HhaI GCGC 1 cut(s) 80
Hin1II CATG 8 cut(s) 32, 228, 347, 351, 355, 578, 614, 762
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HinfI GANTC 3 cut(s) 320, 367, 687
HphI GGTGA 2 cut(s) 172, 626
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 7 cut(s) 37, 147, 172, 313, 325, 379, 591
Hpy188III TCNNGA 3 cut(s) 88, 96, 128
Hpy8I GTNNAC 1 cut(s) 301
HpyAV CCTTC 3 cut(s) 146, 529, 559
HpyCH4III ACNGT 4 cut(s) 56, 166, 407, 503
HpyCH4IV ACGT 2 cut(s) 15, 303
HpyCH4V TGCA 5 cut(s) 44, 347, 351, 448, 553
HpyF3I CTNAG 2 cut(s) 205, 697
HpySE526I ACGT 2 cut(s) 15, 303
Hsp92II CATG 8 cut(s) 32, 228, 347, 351, 355, 578, 614, 762
HspAI GCGC 1 cut(s) 78
KpnI GGTACC 1 cut(s) 220
Kzo9I GATC 5 cut(s) 21, 124, 172, 741, 745
LguI GCTCTTC 1 cut(s) 266
Lsp1109I GCAGC 3 cut(s) 28, 67, 277
LweI GCATC 3 cut(s) 204, 264, 505
MabI ACCWGGT 1 cut(s) 156
MaeII ACGT 2 cut(s) 15, 303
MaeIII GTNAC 4 cut(s) 128, 160, 503, 632
MalI GATC 5 cut(s) 23, 126, 174, 743, 747
MboI GATC 5 cut(s) 21, 124, 172, 741, 745
MboII GAAGA 5 cut(s) 31, 196, 283, 329, 479
MfeI CAATTG 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 95
MluCI AATT 5 cut(s) 211, 492, 548, 595, 724
MlyI GAGTC 2 cut(s) 314, 681
MmeI TCCRAC 3 cut(s) 170, 309, 522
MnlI CCTC 7 cut(s) 127, 286, 463, 538, 553, 564, 665
Mph1103I ATGCAT 2 cut(s) 349, 353
MroXI GAANNNNTTC 1 cut(s) 426
MseI TTAA 3 cut(s) 177, 333, 723
MspA1I CMGCKG 1 cut(s) 268
MspR9I CCNGG 3 cut(s) 113, 158, 248
MunI CAATTG 1 cut(s) 211
MvaI CCWGG 3 cut(s) 113, 158, 248
NdeII GATC 5 cut(s) 21, 124, 172, 741, 745
NlaIII CATG 8 cut(s) 32, 228, 347, 351, 355, 578, 614, 762
NlaIV GGNNCC 2 cut(s) 218, 283
NmuCI GTSAC 3 cut(s) 128, 160, 632
NsiI ATGCAT 2 cut(s) 349, 353
NspI RCATGY 2 cut(s) 351, 355
PaeI GCATGC 2 cut(s) 351, 355
PaeR7I CTCGAG 1 cut(s) 88
PciSI GCTCTTC 1 cut(s) 266
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 1 cut(s) 367
PflMI CCANNNNNTGG 1 cut(s) 247
PkrI GCNGC 3 cut(s) 43, 82, 267
PleI GAGTC 2 cut(s) 314, 681
PpsI GAGTC 2 cut(s) 314, 681
Ppu21I YACGTR 1 cut(s) 304
Psp124BI GAGCTC 1 cut(s) 95
Psp6I CCWGG 3 cut(s) 111, 156, 246
PspEI GGTNACC 1 cut(s) 632
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 3 cut(s) 111, 156, 246
PspN4I GGNNCC 2 cut(s) 218, 283
PspPI GGNCC 1 cut(s) 282
PstI CTGCAG 1 cut(s) 46
PstNI CAGNNNCTG 1 cut(s) 170
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 2 cut(s) 218, 302
RsaNI GTAC 2 cut(s) 217, 301
SacI GAGCTC 1 cut(s) 95
SapI GCTCTTC 1 cut(s) 266
SaqAI TTAA 3 cut(s) 177, 333, 723
SatI GCNGC 3 cut(s) 42, 81, 266
Sau3AI GATC 5 cut(s) 21, 124, 172, 741, 745
Sau96I GGNCC 1 cut(s) 282
SchI GAGTC 2 cut(s) 314, 681
ScrFI CCNGG 3 cut(s) 113, 158, 248
SduI GDGCHC 1 cut(s) 95
SexAI ACCWGGT 1 cut(s) 156
SfaNI GCATC 3 cut(s) 204, 264, 505
SfcI CTRYAG 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 88
SinI GGWCC 1 cut(s) 282
SlaI CTCGAG 1 cut(s) 88
SmlI CTYRAG 2 cut(s) 88, 94
SmoI CTYRAG 2 cut(s) 88, 94
SphI GCATGC 2 cut(s) 351, 355
Sse9I AATT 5 cut(s) 211, 492, 548, 595, 724
SsiI CCGC 1 cut(s) 221
SstI GAGCTC 1 cut(s) 95
StyD4I CCNGG 3 cut(s) 111, 156, 246
StyI CCWWGG 1 cut(s) 734
TaaI ACNGT 4 cut(s) 56, 166, 407, 503
TaiI ACGT 2 cut(s) 18, 306
TaqI TCGA 3 cut(s) 89, 513, 744
TasI AATT 5 cut(s) 211, 492, 548, 595, 724
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 3 cut(s) 177, 333, 723
Tru9I TTAA 3 cut(s) 177, 333, 723
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 3 cut(s) 128, 160, 632
TseI GCWGC 3 cut(s) 41, 80, 265
Tsp45I GTSAC 3 cut(s) 128, 160, 632
TspDTI ATGAA 4 cut(s) 17, 26, 93, 654
TspGWI ACGGA 1 cut(s) 705
TspRI CASTG 1 cut(s) 283
Van91I CCANNNNNTGG 1 cut(s) 247
VpaK11BI GGWCC 1 cut(s) 282
XceI RCATGY 2 cut(s) 351, 355
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XhoI CTCGAG 1 cut(s) 88
XmnI GAANNNNTTC 1 cut(s) 426
Zsp2I ATGCAT 2 cut(s) 349, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.