Rroxscaffold_7G00164600

Nucleoredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009764
Physical Location & Seq
Reverse (-)
6551487 .. 6555651
4165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00164600.1

Sequence Viewer

Length: 609 bp
ATGTCGGAAACCAGTTGGGTGAAAGTTGCTGATCTTGATGGGAAGATAATAGGGCTCTACTTCTCGGCCAATTGGTACCCGCCCTGCTGGAACTTCAACCAAGTCCTAGTTGGTATTTATAATCAGCTTAAGAACAGTAGCAGTACGGGGTCTAGCTTTGAGATTGTGTACATATCATCTGATGAAGACTCTGACGCCTTCAACACCTACCATGCTTGCATGCCGTGGCTGGCGATTCCGTTTTCGGATTTAGAGACAAAGAAAGCTCTGAACCGCAAGTTTGAGATAGAGGGAATTCCCAGCTTGGTTATTCTGCAACCTAGTGATGGTGATGGGGCTACGTTGCATGATGGAGTTGAGCTCATTTATCGATATGGTGTCCAAGCCTTCCCTTTTACTAGACAGAGGCTGGAACAGTTGGAGGAGGAGGAGAAACAGAAGCATGAGAACCAGACCTTGACCAATCTACTAACGAACCACGACAGACACTATCTTCTGGGCCATCAGACGCCTAATCAGGTGCCTGTGGCCTCATTGGTAGGCAAGACAATTGGACTCTACTTCTCAGCTCAATGGTGCATTCCATGCTTTAATTTCACTCGAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 Thioredoxin_8

Protein Analysis

202

Amino Acids

22.97

Weight (kDa)

5.17

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin_8 PF13905 14 - 109 8.4e-22 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 120
Acc65I GGTACC 1 cut(s) 75
AccB1I GGYRCC 2 cut(s) 75, 520
AciI CCGC 2 cut(s) 80, 274
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 1 cut(s) 294
AcyI GRCGYC 2 cut(s) 195, 509
AfaI GTAC 3 cut(s) 77, 145, 170
AfiI CCNNNNNNNGG 1 cut(s) 326
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 2 cut(s) 97, 202
AluBI AGCT 6 cut(s) 127, 156, 266, 303, 361, 569
AluI AGCT 6 cut(s) 127, 156, 266, 303, 361, 569
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 1 cut(s) 248
AlwNI CAGNNNCTG 1 cut(s) 409
Ama87I CYCGRG 1 cut(s) 600
AoxI GGCC 3 cut(s) 66, 499, 528
ApoI RAATTY 1 cut(s) 294
Asp718I GGTACC 1 cut(s) 75
AspS9I GGNCC 1 cut(s) 499
AsuHPI GGTGA 2 cut(s) 31, 341
AvaI CYCGRG 1 cut(s) 600
BanI GGYRCC 2 cut(s) 75, 520
BanII GRGCYC 2 cut(s) 57, 363
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 363
BccI CCATC 5 cut(s) 32, 320, 326, 344, 510
BceAI ACGGC 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 248
BfaI CTAG 4 cut(s) 107, 153, 321, 399
BfrI CTTAAG 1 cut(s) 128
BmeT110I CYCGRG 1 cut(s) 600
BmgT120I GGNCC 1 cut(s) 499
BmiI GGNNCC 2 cut(s) 77, 522
BpiI GAAGAC 1 cut(s) 192
BplI GAGNNNNNCTC 2 cut(s) 345, 377
Bsa29I ATCGAT 1 cut(s) 370
BsaHI GRCGYC 2 cut(s) 195, 509
BsaJI CCNNGG 1 cut(s) 224
Bsc4I CCNNNNNNNGG 1 cut(s) 326
Bse1I ACTGG 1 cut(s) 12
BseCI ATCGAT 1 cut(s) 370
BseDI CCNNGG 1 cut(s) 224
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMII CTCAG 1 cut(s) 579
BseNI ACTGG 1 cut(s) 12
BseRI GAGGAG 3 cut(s) 437, 440, 443
BseYI CCCAGC 1 cut(s) 299
BshFI GGCC 3 cut(s) 68, 501, 530
BshNI GGYRCC 2 cut(s) 75, 520
BshVI ATCGAT 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 363
BsiHKCI CYCGRG 1 cut(s) 600
BslI CCNNNNNNNGG 1 cut(s) 326
BsmAI GTCTC 1 cut(s) 248
BsmI GAATGC 1 cut(s) 579
BsnI GGCC 3 cut(s) 68, 501, 530
BsoBI CYCGRG 1 cut(s) 600
Bsp1286I GDGCHC 2 cut(s) 57, 363
Bsp1407I TGTACA 1 cut(s) 168
Bsp143I GATC 1 cut(s) 31
BspACI CCGC 2 cut(s) 80, 274
BspANI GGCC 3 cut(s) 68, 501, 530
BspCNI CTCAG 1 cut(s) 578
BspDI ATCGAT 1 cut(s) 370
BspLI GGNNCC 2 cut(s) 77, 522
BspT107I GGYRCC 2 cut(s) 75, 520
BspTI CTTAAG 1 cut(s) 128
BsrGI TGTACA 1 cut(s) 168
BsrI ACTGG 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 224
BssMI GATC 1 cut(s) 31
BssNI GRCGYC 2 cut(s) 195, 509
Bst4CI ACNGT 2 cut(s) 137, 417
BstACI GRCGYC 2 cut(s) 195, 509
BstAFI CTTAAG 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 585
BstAUI TGTACA 1 cut(s) 168
BstC8I GCNNGC 3 cut(s) 217, 221, 231
BstDEI CTNAG 1 cut(s) 565
BstDSI CCRYGG 1 cut(s) 224
BstKTI GATC 1 cut(s) 34
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 1 cut(s) 31
BstMWI GCNNNNNNNGC 1 cut(s) 585
BstNSI RCATGY 1 cut(s) 223
BstV2I GAAGAC 1 cut(s) 192
Bsu15I ATCGAT 1 cut(s) 370
BsuRI GGCC 3 cut(s) 68, 501, 530
BsuTUI ATCGAT 1 cut(s) 370
BtgI CCRYGG 1 cut(s) 224
Cac8I GCNNGC 3 cut(s) 217, 221, 231
CaiI CAGNNNCTG 1 cut(s) 409
Cfr13I GGNCC 1 cut(s) 499
ClaI ATCGAT 1 cut(s) 370
CseI GACGC 2 cut(s) 203, 517
Csp6I GTAC 3 cut(s) 76, 144, 169
CviAII CATG 5 cut(s) 212, 220, 347, 443, 585
CviQI GTAC 3 cut(s) 76, 144, 169
DdeI CTNAG 1 cut(s) 565
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 66
Ecl136II GAGCTC 1 cut(s) 361
Eco24I GRGCYC 2 cut(s) 57, 363
Eco53kI GAGCTC 1 cut(s) 361
Eco88I CYCGRG 1 cut(s) 600
EcoICRI GAGCTC 1 cut(s) 361
EcoRI GAATTC 1 cut(s) 294
EcoT38I GRGCYC 2 cut(s) 57, 363
FaeI CATG 5 cut(s) 215, 223, 350, 446, 588
FaiI YATR 8 cut(s) 120, 173, 213, 221, 348, 375, 444, 586
FatI CATG 5 cut(s) 211, 219, 346, 442, 584
FauI CCCGC 1 cut(s) 87
FriOI GRGCYC 2 cut(s) 57, 363
FspBI CTAG 4 cut(s) 107, 153, 321, 399
GsaI CCCAGC 1 cut(s) 303
HaeIII GGCC 3 cut(s) 68, 501, 530
HgaI GACGC 2 cut(s) 203, 517
Hin1I GRCGYC 2 cut(s) 195, 509
Hin1II CATG 5 cut(s) 215, 223, 350, 446, 588
HinfI GANTC 3 cut(s) 188, 235, 555
HphI GGTGA 2 cut(s) 31, 341
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 6 cut(s) 7, 181, 193, 247, 270, 507
Hpy188III TCNNGA 1 cut(s) 35
Hpy8I GTNNAC 1 cut(s) 169
HpyAV CCTTC 2 cut(s) 208, 397
HpyCH4III ACNGT 2 cut(s) 137, 417
HpyCH4IV ACGT 1 cut(s) 341
HpyCH4V TGCA 4 cut(s) 219, 316, 346, 579
HpyF10VI GCNNNNNNNGC 1 cut(s) 585
HpyF3I CTNAG 1 cut(s) 565
HpySE526I ACGT 1 cut(s) 341
Hsp92I GRCGYC 2 cut(s) 195, 509
Hsp92II CATG 5 cut(s) 215, 223, 350, 446, 588
KpnI GGTACC 1 cut(s) 79
Kzo9I GATC 1 cut(s) 31
MaeI CTAG 4 cut(s) 107, 153, 321, 399
MaeII ACGT 1 cut(s) 341
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MboII GAAGA 3 cut(s) 55, 197, 485
MfeI CAATTG 2 cut(s) 70, 549
MhlI GDGCHC 2 cut(s) 57, 363
MluCI AATT 4 cut(s) 70, 294, 549, 592
MlyI GAGTC 2 cut(s) 182, 549
MmeI TCCRAC 1 cut(s) 399
MnlI CCTC 7 cut(s) 283, 399, 415, 418, 421, 541, 596
MseI TTAA 2 cut(s) 129, 591
MspCI CTTAAG 1 cut(s) 128
MunI CAATTG 2 cut(s) 70, 549
Mva1269I GAATGC 1 cut(s) 579
MwoI GCNNNNNNNGC 1 cut(s) 585
NdeII GATC 1 cut(s) 31
NlaIII CATG 5 cut(s) 215, 223, 350, 446, 588
NlaIV GGNNCC 2 cut(s) 77, 522
NmeAIII GCCGAG 1 cut(s) 44
NspI RCATGY 1 cut(s) 223
PaeI GCATGC 1 cut(s) 223
PaeR7I CTCGAG 1 cut(s) 600
PctI GAATGC 1 cut(s) 579
PfeI GAWTC 1 cut(s) 235
PleI GAGTC 2 cut(s) 182, 549
PpsI GAGTC 2 cut(s) 182, 549
PsiI TTATAA 1 cut(s) 120
Psp124BI GAGCTC 1 cut(s) 363
PspFI CCCAGC 1 cut(s) 299
PspN4I GGNNCC 2 cut(s) 77, 522
PspPI GGNCC 1 cut(s) 499
PspXI VCTCGAGB 1 cut(s) 600
PstNI CAGNNNCTG 1 cut(s) 409
RsaI GTAC 3 cut(s) 77, 145, 170
RsaNI GTAC 3 cut(s) 76, 144, 169
SacI GAGCTC 1 cut(s) 363
SaqAI TTAA 2 cut(s) 129, 591
Sau3AI GATC 1 cut(s) 31
Sau96I GGNCC 1 cut(s) 499
SchI GAGTC 2 cut(s) 182, 549
SduI GDGCHC 2 cut(s) 57, 363
Sfr274I CTCGAG 1 cut(s) 600
SlaI CTCGAG 1 cut(s) 600
SmlI CTYRAG 2 cut(s) 128, 600
SmoI CTYRAG 2 cut(s) 128, 600
SphI GCATGC 1 cut(s) 223
Sse9I AATT 4 cut(s) 70, 294, 549, 592
SsiI CCGC 2 cut(s) 80, 274
SspMI CTAG 4 cut(s) 107, 153, 321, 399
SstI GAGCTC 1 cut(s) 363
TaaI ACNGT 2 cut(s) 137, 417
TaiI ACGT 1 cut(s) 344
TaqI TCGA 2 cut(s) 370, 601
TasI AATT 4 cut(s) 70, 294, 549, 592
TatI WGTACW 1 cut(s) 168
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 2 cut(s) 129, 591
Tru9I TTAA 2 cut(s) 129, 591
TspDTI ATGAA 1 cut(s) 198
TspGWI ACGGA 1 cut(s) 228
Vha464I CTTAAG 1 cut(s) 128
XapI RAATTY 1 cut(s) 294
XceI RCATGY 1 cut(s) 223
XcmI CCANNNNNNNNNTGG 1 cut(s) 107
XhoI CTCGAG 1 cut(s) 600
XspI CTAG 4 cut(s) 107, 153, 321, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.