Rorug06G0322600

Nucleoredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009764
Physical Location & Seq
Forward (+)
47423428 .. 47428726
5299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0322600.1

Sequence Viewer

Length: 2208 bp
ATGGCGTCAGTGGCCACCGTCCTCCGCCGGAAGCTCTCCGGCAAGCCATTCCATTTCCGATTACTACCAGTCCGATCATCCTCCGACTCGCCGCAGAGAATCGAGAAGATTCTGATAGCCAACCGGGGCGAAATTGCGTGCAGGATCATGAGGACCGCAAAGAGACTCGGAATTCAAACCGTCGCCGTTTTCAGCGACCCCGATAGGCACTCGCTTCACGTCAAATCCGCCGACGAGGCCGTTCGTATCGGTCCGGCTCCGGCTCGGCTCAGCTACCTCGACGCCTCGTCGATTCTTGATGCCGCCGTTCGTACTGGTGCGCAGGCTATCCATCCTGGCTATGGTTTTCTGTCAGAGAGTTCTGACTTTGCTCAACTTTGTGAGGATAAGGGTCTTACCTTTATTGGGCCTCCAGCATCTGCCATCAGGGACATGGGTGACAAAAGTGCGTCAAAGAGGATAATGGGTGCAGCAGGGGTACCTCTTGTGCCTGGATATCATGGGAAAGACCAAGATATTGATCTAATGAAGATAGAAGCCGACAAGATTGGATATCCAATCTTAATAAAGCCAACTCATGGAGGTGGGGGAAAGGGTATGAGGATTGTGCAAAGTCCGGATGAGTTTGTTGAAGCCTTCCTTGGAGCACAACGTGAGGCTGCTGCTTCTTTTGGCGTAAACACAATTTTGCTGGAGAAATATATTACACAGCCAAGACACATAGAAGTCCAGATATTTGGGGATAAACACGGGAATGTTCTACATTTATATGAGAGAGATTGCAGCGTTCAAAGAAGGCACCAGAAAATAATCGAAGAAGCCCCAGCTCCAAATGTTTCTGATGACTTCCGGTCTCACTTGGGTCAAGCGGCTGTATCTGCTGCCAAGGCAGTTAGCTATCACAGTGCCGGCACAGTGGAGTTTATAGTCGATACAGTCTCAGGCCAATTTTATTTCATGGAGATGAATACCCGTCTTCAGGTTGAGCATCCTGTGACTGAGATGATTGTTGGCCAAGATCTTGTAGAGTGGCAAATTCGTGTAGCAAATGGAGAACACCTTCCCATTAGTCAGTCACAGGTCCCTTTGTCAGGTCATGCTTTTGAAGCCCGTATATATGCTGAAAATGTACCAAAAGGTTTCCTTCCTGCAACTGGAGTTCTTCATCACTATCAGCATGCTCCAGTTTCACCATCAGTTCGAGTTGAGACTGGAGTTGAACAAGGAGACAGTGTTAGCATGCATTATGATCCTATGATTGCCAAGCTTGTAGTATGGGGAGAAAATCGTGCAGTAGCACTGGTAAAACTGAAGGATTGCTTGACAAAGTTTCAGGTTGCAGGTGTACCAACCAACATCAGTTTTCTGTCAAAACTTGCCAACCATCGGGAATTTGAAAATGGGAATGTGGAAACTCATTTTATTGAACATTTTAAAGATGACCTGTTTGTCAACCCTAGTAATTTGGAAGTAATGAACACAGTGCTTGGTGCTGCTAGATTTGGTGCCAAACTGGCGGCTGCATGTCTCATTGAAAAGGAAAATTCTGTATTCAGAGAAAATCTTCCTGGAAGCAACAACACGATCTCCATATGGTATTCTTCTCCGCCTTTCAGAGTCAATCATTGTGCTAGGCATACAAAGGAACTCGAGTGGGAGAATGAATATGATAGCAGTGGCTCAAAGTTATTGACCTATTCCATCACTTACAAATCAGATGGGAGCTATCTAATTGAGACACAAGAAGACATTTCCCCACCTTTGGAGGTTAAAGCAACTTGTGTAGGTGACCATGATTTCAAAGTTGAAGCTGCTGGTGTAATCATGGATGTTAGCTTAGCTGTTTACTCGAAGGATCAGACCAAGCACATTCATATGTGGCATGGGTCACATCATCATCATTTCAGACAAAAATTGGGTCTTGAATTGTCCAATGAAGATGAAACCGAACATAGGCCCAGTTTTGATAGATCATCTCATCCTCCGGGGACAGTTGTTGCACCCATGGCTGGTTTGGTGGTCAAGGTTCTTATTAAGGATGCAACAAAAGTGGAGGAAGGACAACCTATTTTAGTATTAGAGGCAATGAAGATGGAGCATGTTGTAAAAGCACCATCAGCAGGCTATGTCCACGGGCTTCATCTAGCAGCTGGCCAGCAGGTTTCTGATGGCAGTGTTCTCTTCAGTATTAAGGAGTATTCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 Thioredoxin_8

Protein Analysis

735

Amino Acids

80.87

Weight (kDa)

6.33

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 34 - 142 7.9e-45 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 147 - 355 1.9e-74 Carbamoyl-phosphate synthase L chain, ATP binding domain
Dala_Dala_lig_C PF07478 174 - 324 4.2e-10 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 369 - 476 5.9e-36 Biotin carboxylase C-terminal domain
MCCA_BT PF19331 489 - 652 4.3e-78 Methylcrotonoyl-CoA carboxylase subunit alpha BT domain
Biotin_lipoyl PF00364 664 - 730 6.5e-16 Biotin-requiring enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1331
AasI GACNNNNNNGTC 1 cut(s) 1448
Acc16I TGCGCA 1 cut(s) 321
Acc36I ACCTGC 2 cut(s) 1331, 2149
Acc65I GGTACC 1 cut(s) 478
AccB1I GGYRCC 3 cut(s) 478, 798, 1505
AccB7I CCANNNNNTGG 1 cut(s) 578
AccIII TCCGGA 1 cut(s) 616
AciI CCGC 8 cut(s) 25, 92, 156, 228, 303, 869, 1517, 1607
AclWI GGATC 3 cut(s) 152, 1244, 1863
AcoI YGGCCR 3 cut(s) 12, 1012, 2152
AcsI RAATTY 4 cut(s) 171, 1035, 1391, 1543
AcuI CTGAAG 3 cut(s) 962, 1331, 2167
AcyI GRCGYC 2 cut(s) 5, 282
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 4 cut(s) 313, 480, 1131, 1347
AhdI GACNNNNNGTC 2 cut(s) 286, 850
AjiI CACGTC 1 cut(s) 220
AjnI CCWGG 3 cut(s) 334, 490, 1567
AjuI GAANNNNNNNTTGG 2 cut(s) 624, 656
Alw21I GWGCWC 1 cut(s) 649
Alw26I GTCTC 7 cut(s) 157, 858, 943, 1202, 1221, 1532, 1730
AlwI GGATC 3 cut(s) 152, 1244, 1863
AlwNI CAGNNNCTG 1 cut(s) 419
Ama87I CYCGRG 1 cut(s) 1649
Aor13HI TCCGGA 1 cut(s) 616
AoxI GGCC 7 cut(s) 12, 237, 407, 943, 1012, 1955, 2152
ApeKI GCWGC 9 cut(s) 470, 659, 662, 783, 881, 1493, 1520, 1811, 2147
ApoI RAATTY 4 cut(s) 171, 1035, 1391, 1543
ArsI GACNNNNNNTTYG 2 cut(s) 1903, 1935
Asp700I GAANNNNTTC 2 cut(s) 1059, 1563
Asp718I GGTACC 1 cut(s) 478
AspLEI GCGC 1 cut(s) 322
AspS9I GGNCC 5 cut(s) 153, 251, 407, 1081, 1956
AsuC2I CCSGG 2 cut(s) 125, 1986
AsuHPI GGTGA 3 cut(s) 449, 1182, 1799
AvaI CYCGRG 1 cut(s) 1649
AvaII GGWCC 3 cut(s) 153, 251, 1081
BalI TGGCCA 3 cut(s) 14, 1014, 2154
BanI GGYRCC 3 cut(s) 478, 798, 1505
BbsI GAAGAC 2 cut(s) 968, 1752
Bbv12I GWGCWC 1 cut(s) 649
BbvI GCAGC 9 cut(s) 482, 646, 649, 795, 868, 1480, 1507, 1798, 2159
BccI CCATC 9 cut(s) 339, 431, 1201, 1392, 1709, 1712, 2086, 2122, 2162
BceAI ACGGC 3 cut(s) 170, 224, 290
BciT130I CCWGG 3 cut(s) 336, 492, 1569
BcnI CCSGG 2 cut(s) 125, 1986
BcoDI GTCTC 7 cut(s) 157, 858, 943, 1202, 1221, 1532, 1730
BfaI CTAG 4 cut(s) 1458, 1497, 1632, 2144
BfuAI ACCTGC 2 cut(s) 1331, 2149
BglI GCCNNNNNGGC 2 cut(s) 236, 1514
BglII AGATCT 1 cut(s) 1018
BlpI GCTNAGC 2 cut(s) 269, 1837
Bme1390I CCNGG 5 cut(s) 125, 336, 492, 1569, 1986
Bme18I GGWCC 3 cut(s) 153, 251, 1081
BmeRI GACNNNNNGTC 2 cut(s) 286, 850
BmeT110I CYCGRG 1 cut(s) 1649
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 5 cut(s) 153, 251, 407, 1081, 1956
BmiI GGNNCC 5 cut(s) 258, 480, 800, 1083, 1507
BmrFI CCNGG 5 cut(s) 125, 336, 492, 1569, 1986
BmrI ACTGGG 1 cut(s) 1953
BmsI GCATC 4 cut(s) 289, 425, 997, 2029
BmuI ACTGGG 1 cut(s) 1953
BpiI GAAGAC 2 cut(s) 968, 1752
BpmI CTGGAG 5 cut(s) 396, 713, 1167, 1176, 1233
Bpu1102I GCTNAGC 2 cut(s) 269, 1837
BpuMI CCSGG 2 cut(s) 125, 1986
BsaBI GATNNNNATC 1 cut(s) 519
BsaHI GRCGYC 2 cut(s) 5, 282
BsaI GGTCTC 1 cut(s) 858
BsaJI CCNNGG 6 cut(s) 124, 640, 885, 1985, 2004, 2131
BsaWI WCCGGW 2 cut(s) 616, 849
BsaXI ACNNNNNCTCC 4 cut(s) 1218, 1248, 1571, 1601
Bse118I RCCGGY 1 cut(s) 908
Bse1I ACTGG 8 cut(s) 68, 319, 1159, 1184, 1216, 1305, 1518, 1959
Bse3DI GCAATG 1 cut(s) 2091
Bse8I GATNNNNATC 1 cut(s) 519
BseAI TCCGGA 1 cut(s) 616
BseBI CCWGG 3 cut(s) 336, 492, 1569
BseDI CCNNGG 6 cut(s) 124, 640, 885, 1985, 2004, 2131
BseGI GGATG 7 cut(s) 77, 331, 625, 988, 1834, 1978, 2044
BseJI GATNNNNATC 1 cut(s) 519
BseMI GCAATG 1 cut(s) 2091
BseMII CTCAG 3 cut(s) 283, 954, 990
BseNI ACTGG 8 cut(s) 68, 319, 1159, 1184, 1216, 1305, 1518, 1959
BseXI GCAGC 9 cut(s) 482, 646, 649, 795, 868, 1480, 1507, 1798, 2159
BseYI CCCAGC 1 cut(s) 823
BsgI GTGCAG 3 cut(s) 160, 489, 1311
BshFI GGCC 7 cut(s) 14, 239, 409, 945, 1014, 1957, 2154
BshNI GGYRCC 3 cut(s) 478, 798, 1505
BsiHKAI GWGCWC 1 cut(s) 649
BsiHKCI CYCGRG 1 cut(s) 1649
BsiSI CCGG 9 cut(s) 28, 39, 124, 254, 260, 617, 850, 909, 1985
BslFI GGGAC 3 cut(s) 443, 1067, 2002
BsmAI GTCTC 7 cut(s) 157, 858, 943, 1202, 1221, 1532, 1730
BsmFI GGGAC 3 cut(s) 443, 1067, 2002
BsnI GGCC 7 cut(s) 14, 239, 409, 945, 1014, 1957, 2154
Bso31I GGTCTC 1 cut(s) 858
BsoBI CYCGRG 1 cut(s) 1649
Bsp1286I GDGCHC 1 cut(s) 649
Bsp13I TCCGGA 1 cut(s) 616
Bsp143I GATC 8 cut(s) 74, 144, 520, 1018, 1249, 1584, 1855, 1970
Bsp1720I GCTNAGC 2 cut(s) 269, 1837
Bsp19I CCATGG 1 cut(s) 2004
BspACI CCGC 8 cut(s) 25, 92, 156, 228, 303, 869, 1517, 1607
BspANI GGCC 7 cut(s) 14, 239, 409, 945, 1014, 1957, 2154
BspCNI CTCAG 3 cut(s) 282, 953, 991
BspEI TCCGGA 1 cut(s) 616
BspHI TCATGA 1 cut(s) 147
BspLI GGNNCC 5 cut(s) 258, 480, 800, 1083, 1507
BspMI ACCTGC 2 cut(s) 1331, 2149
BspPI GGATC 3 cut(s) 152, 1244, 1863
BspT107I GGYRCC 3 cut(s) 478, 798, 1505
BspTNI GGTCTC 1 cut(s) 858
BsrDI GCAATG 1 cut(s) 2091
BsrFI RCCGGY 1 cut(s) 908
BsrI ACTGG 8 cut(s) 68, 319, 1159, 1184, 1216, 1305, 1518, 1959
BssAI RCCGGY 1 cut(s) 908
BssECI CCNNGG 6 cut(s) 124, 640, 885, 1985, 2004, 2131
BssMI GATC 8 cut(s) 74, 144, 520, 1018, 1249, 1584, 1855, 1970
BssNI GRCGYC 2 cut(s) 5, 282
BssT1I CCWWGG 3 cut(s) 640, 885, 2004
Bst2UI CCWGG 3 cut(s) 336, 492, 1569
Bst4CI ACNGT 9 cut(s) 19, 181, 905, 916, 937, 1232, 1483, 1993, 2205
Bst6I CTCTTC 1 cut(s) 2186
BstACI GRCGYC 2 cut(s) 5, 282
BstC8I GCNNGC 9 cut(s) 44, 139, 324, 910, 1179, 1241, 2122, 2152, 2156
BstDEI CTNAG 4 cut(s) 269, 940, 999, 1837
BstDSI CCRYGG 2 cut(s) 2004, 2131
BstEII GGTNACC 1 cut(s) 1787
BstENI CCTNNNNNAGG 1 cut(s) 1089
BstF5I GGATG 7 cut(s) 77, 331, 625, 988, 1834, 1978, 2044
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 8 cut(s) 77, 147, 523, 1021, 1252, 1587, 1858, 1973
BstMAI GTCTC 7 cut(s) 157, 858, 943, 1202, 1221, 1532, 1730
BstMBI GATC 8 cut(s) 74, 144, 520, 1018, 1249, 1584, 1855, 1970
BstMWI GCNNNNNNNGC 8 cut(s) 11, 236, 878, 887, 1106, 1514, 2006, 2117
BstNI CCWGG 3 cut(s) 336, 492, 1569
BstNSI RCATGY 4 cut(s) 1181, 1243, 1527, 2102
BstPI GGTNACC 1 cut(s) 1787
BstSCI CCNGG 5 cut(s) 123, 334, 490, 1567, 1984
BstV1I GCAGC 9 cut(s) 482, 646, 649, 795, 868, 1480, 1507, 1798, 2159
BstV2I GAAGAC 2 cut(s) 968, 1752
BstX2I RGATCY 1 cut(s) 1018
BstXI CCANNNNNNTGG 1 cut(s) 737
BstYI RGATCY 1 cut(s) 1018
BsuRI GGCC 7 cut(s) 14, 239, 409, 945, 1014, 1957, 2154
BtgI CCRYGG 2 cut(s) 2004, 2131
BtrI CACGTC 1 cut(s) 220
BtsCI GGATG 7 cut(s) 77, 331, 625, 988, 1834, 1978, 2044
BtsI GCAGTG 2 cut(s) 1681, 2179
BtsIMutI CAGTG 8 cut(s) 15, 910, 921, 1237, 1298, 1488, 1681, 2179
BveI ACCTGC 2 cut(s) 1331, 2149
Cac8I GCNNGC 9 cut(s) 44, 139, 324, 910, 1179, 1241, 2122, 2152, 2156
CaiI CAGNNNCTG 1 cut(s) 419
CciI TCATGA 1 cut(s) 147
CfoI GCGC 1 cut(s) 322
Cfr10I RCCGGY 1 cut(s) 908
Cfr13I GGNCC 5 cut(s) 153, 251, 407, 1081, 1956
CpoI CGGWCCG 1 cut(s) 251
CseI GACGC 2 cut(s) 290, 438
Csp6I GTAC 4 cut(s) 312, 479, 1130, 1346
CspCI CAANNNNNGTGG 2 cut(s) 2031, 2066
CspI CGGWCCG 1 cut(s) 251
CviQI GTAC 4 cut(s) 312, 479, 1130, 1346
DdeI CTNAG 4 cut(s) 269, 940, 999, 1837
DpnI GATC 8 cut(s) 76, 146, 522, 1020, 1251, 1586, 1857, 1972
DpnII GATC 8 cut(s) 74, 144, 520, 1018, 1249, 1584, 1855, 1970
DraI TTTAAA 1 cut(s) 1435
DraIII CACNNNGTG 1 cut(s) 653
DrdI GACNNNNNNGTC 1 cut(s) 1448
DriI GACNNNNNGTC 2 cut(s) 286, 850
DseDI GACNNNNNNGTC 1 cut(s) 1448
EaeI YGGCCR 3 cut(s) 12, 1012, 2152
Eam1104I CTCTTC 1 cut(s) 2186
Eam1105I GACNNNNNGTC 2 cut(s) 286, 850
EarI CTCTTC 1 cut(s) 2186
EciI GGCGGA 3 cut(s) 14, 217, 1596
Eco130I CCWWGG 3 cut(s) 640, 885, 2004
Eco31I GGTCTC 1 cut(s) 858
Eco32I GATATC 2 cut(s) 497, 554
Eco47I GGWCC 3 cut(s) 153, 251, 1081
Eco57I CTGAAG 3 cut(s) 962, 1331, 2167
Eco88I CYCGRG 1 cut(s) 1649
Eco91I GGTNACC 1 cut(s) 1787
EcoNI CCTNNNNNAGG 1 cut(s) 1089
EcoO109I RGGNCCY 1 cut(s) 1081
EcoO65I GGTNACC 1 cut(s) 1787
EcoRI GAATTC 1 cut(s) 171
EcoRII CCWGG 3 cut(s) 334, 490, 1567
EcoRV GATATC 2 cut(s) 497, 554
EcoT14I CCWWGG 3 cut(s) 640, 885, 2004
EcoT22I ATGCAT 1 cut(s) 1245
ErhI CCWWGG 3 cut(s) 640, 885, 2004
FalI AAGNNNNNCTT 6 cut(s) 624, 656, 1128, 1160, 1304, 1336
FaqI GGGAC 3 cut(s) 443, 1067, 2002
FauNDI CATATG 2 cut(s) 1592, 1875
FokI GGATG 7 cut(s) 64, 318, 632, 975, 1841, 1965, 2051
FspBI CTAG 4 cut(s) 1458, 1497, 1632, 2144
FspI TGCGCA 1 cut(s) 321
GlaI GCGC 1 cut(s) 321
GsaI CCCAGC 1 cut(s) 827
GsuI CTGGAG 5 cut(s) 396, 713, 1167, 1176, 1233
HaeIII GGCC 7 cut(s) 14, 239, 409, 945, 1014, 1957, 2154
HapII CCGG 9 cut(s) 28, 39, 124, 254, 260, 617, 850, 909, 1985
HgaI GACGC 2 cut(s) 290, 438
HhaI GCGC 1 cut(s) 322
Hin1I GRCGYC 2 cut(s) 5, 282
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HincII GTYRAC 1 cut(s) 1453
HindII GTYRAC 1 cut(s) 1453
HindIII AAGCTT 1 cut(s) 1265
HinfI GANTC 6 cut(s) 86, 99, 109, 165, 292, 1617
HpaII CCGG 9 cut(s) 28, 39, 124, 254, 260, 617, 850, 909, 1985
HphI GGTGA 3 cut(s) 449, 1182, 1799
Hpy166II GTNNAC 5 cut(s) 679, 1346, 1453, 1846, 2131
Hpy188III TCNNGA 7 cut(s) 103, 148, 296, 617, 730, 1388, 1922
Hpy8I GTNNAC 5 cut(s) 679, 1346, 1453, 1846, 2131
Hpy99I CGWCG 4 cut(s) 185, 236, 284, 292
HpyAV CCTTC 7 cut(s) 646, 789, 1070, 1154, 1306, 1846, 2051
HpyCH4III ACNGT 9 cut(s) 19, 181, 905, 916, 937, 1232, 1483, 1993, 2205
HpyCH4IV ACGT 2 cut(s) 219, 652
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 236, 878, 887, 1106, 1514, 2006, 2117
HpyF3I CTNAG 4 cut(s) 269, 940, 999, 1837
HpySE526I ACGT 2 cut(s) 219, 652
Hsp92I GRCGYC 2 cut(s) 5, 282
HspAI GCGC 1 cut(s) 320
Kpn2I TCCGGA 1 cut(s) 616
KpnI GGTACC 1 cut(s) 482
KroI GCCGGC 1 cut(s) 908
KroNI GCCGGC 1 cut(s) 910
Kzo9I GATC 8 cut(s) 74, 144, 520, 1018, 1249, 1584, 1855, 1970
LmnI GCTCC 6 cut(s) 262, 644, 832, 1186, 1722, 2095
Lsp1109I GCAGC 9 cut(s) 482, 646, 649, 795, 868, 1480, 1507, 1798, 2159
LweI GCATC 4 cut(s) 289, 425, 997, 2029
MaeI CTAG 4 cut(s) 1458, 1497, 1632, 2144
MaeII ACGT 2 cut(s) 219, 652
MaeIII GTNAC 5 cut(s) 437, 994, 1074, 1787, 1887
MalI GATC 8 cut(s) 76, 146, 522, 1020, 1251, 1586, 1857, 1972
MboI GATC 8 cut(s) 74, 144, 520, 1018, 1249, 1584, 1855, 1970
MflI RGATCY 1 cut(s) 1018
MhlI GDGCHC 1 cut(s) 649
MlsI TGGCCA 3 cut(s) 14, 1014, 2154
MluNI TGGCCA 3 cut(s) 14, 1014, 2154
MlyI GAGTC 3 cut(s) 80, 159, 1626
MmeI TCCRAC 1 cut(s) 108
Mox20I TGGCCA 3 cut(s) 14, 1014, 2154
Mph1103I ATGCAT 1 cut(s) 1245
MroI TCCGGA 1 cut(s) 616
MroNI GCCGGC 1 cut(s) 908
MroXI GAANNNNTTC 2 cut(s) 1059, 1563
MscI TGGCCA 3 cut(s) 14, 1014, 2154
MseI TTAA 5 cut(s) 563, 1434, 1770, 2034, 2190
MslI CAYNNNNRTG 5 cut(s) 582, 753, 768, 962, 1874
Msp20I TGGCCA 3 cut(s) 14, 1014, 2154
MspA1I CMGCKG 1 cut(s) 2150
MspI CCGG 9 cut(s) 28, 39, 124, 254, 260, 617, 850, 909, 1985
MspR9I CCNGG 5 cut(s) 125, 336, 492, 1569, 1986
MvaI CCWGG 3 cut(s) 336, 492, 1569
MwoI GCNNNNNNNGC 8 cut(s) 11, 236, 878, 887, 1106, 1514, 2006, 2117
NaeI GCCGGC 1 cut(s) 910
NciI CCSGG 2 cut(s) 125, 1986
NcoI CCATGG 1 cut(s) 2004
NdeI CATATG 2 cut(s) 1592, 1875
NdeII GATC 8 cut(s) 74, 144, 520, 1018, 1249, 1584, 1855, 1970
NgoMIV GCCGGC 1 cut(s) 908
NlaIV GGNNCC 5 cut(s) 258, 480, 800, 1083, 1507
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 5 cut(s) 437, 994, 1074, 1787, 1887
NsbI TGCGCA 1 cut(s) 321
NsiI ATGCAT 1 cut(s) 1245
NspI RCATGY 4 cut(s) 1181, 1243, 1527, 2102
PaeI GCATGC 2 cut(s) 1181, 1243
PaeR7I CTCGAG 1 cut(s) 1649
PagI TCATGA 1 cut(s) 147
PaqCI CACCTGC 1 cut(s) 1331
PdiI GCCGGC 1 cut(s) 910
PdmI GAANNNNTTC 2 cut(s) 1059, 1563
PfeI GAWTC 3 cut(s) 99, 109, 292
PflMI CCANNNNNTGG 1 cut(s) 578
PfoI TCCNGGA 1 cut(s) 1567
PleI GAGTC 3 cut(s) 80, 159, 1625
PpsI GAGTC 3 cut(s) 80, 159, 1625
PpuMI RGGWCCY 1 cut(s) 1081
Psp5II RGGWCCY 1 cut(s) 1081
Psp6I CCWGG 3 cut(s) 334, 490, 1567
PspEI GGTNACC 1 cut(s) 1787
PspFI CCCAGC 1 cut(s) 823
PspGI CCWGG 3 cut(s) 334, 490, 1567
PspN4I GGNNCC 5 cut(s) 258, 480, 800, 1083, 1507
PspPI GGNCC 5 cut(s) 153, 251, 407, 1081, 1956
PspPPI RGGWCCY 1 cut(s) 1081
PspXI VCTCGAGB 1 cut(s) 1649
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 1 cut(s) 1018
PvuII CAGCTG 1 cut(s) 2150
RsaI GTAC 4 cut(s) 313, 480, 1131, 1347
RsaNI GTAC 4 cut(s) 312, 479, 1130, 1346
RseI CAYNNNNRTG 5 cut(s) 582, 753, 768, 962, 1874
Rsr2I CGGWCCG 1 cut(s) 251
RsrII CGGWCCG 1 cut(s) 251
SaqAI TTAA 5 cut(s) 563, 1434, 1770, 2034, 2190
Sau3AI GATC 8 cut(s) 74, 144, 520, 1018, 1249, 1584, 1855, 1970
Sau96I GGNCC 5 cut(s) 153, 251, 407, 1081, 1956
SchI GAGTC 3 cut(s) 80, 159, 1626
ScrFI CCNGG 5 cut(s) 125, 336, 492, 1569, 1986
SduI GDGCHC 1 cut(s) 649
SfaNI GCATC 4 cut(s) 289, 425, 997, 2029
Sfr274I CTCGAG 1 cut(s) 1649
SinI GGWCC 3 cut(s) 153, 251, 1081
SlaI CTCGAG 1 cut(s) 1649
SmiMI CAYNNNNRTG 5 cut(s) 582, 753, 768, 962, 1874
SmlI CTYRAG 1 cut(s) 1649
SmoI CTYRAG 1 cut(s) 1649
SphI GCATGC 2 cut(s) 1181, 1243
SsiI CCGC 8 cut(s) 25, 92, 156, 228, 303, 869, 1517, 1607
SspMI CTAG 4 cut(s) 1458, 1497, 1632, 2144
StyD4I CCNGG 5 cut(s) 123, 334, 490, 1567, 1984
StyI CCWWGG 3 cut(s) 640, 885, 2004
TaaI ACNGT 9 cut(s) 19, 181, 905, 916, 937, 1232, 1483, 1993, 2205
TaiI ACGT 2 cut(s) 222, 655
TaqI TCGA 8 cut(s) 102, 279, 290, 813, 930, 1201, 1650, 1850
TaqII GACCGA 1 cut(s) 239
TauI GCSGC 4 cut(s) 94, 305, 872, 1520
TfiI GAWTC 3 cut(s) 99, 109, 292
Tru1I TTAA 5 cut(s) 563, 1434, 1770, 2034, 2190
Tru9I TTAA 5 cut(s) 563, 1434, 1770, 2034, 2190
TscAI CASTG 8 cut(s) 15, 910, 921, 1237, 1305, 1488, 1681, 2179
TseFI GTSAC 5 cut(s) 437, 994, 1074, 1787, 1887
TseI GCWGC 9 cut(s) 470, 659, 662, 783, 881, 1493, 1520, 1811, 2147
Tsp45I GTSAC 5 cut(s) 437, 994, 1074, 1787, 1887
TspRI CASTG 8 cut(s) 15, 910, 921, 1237, 1305, 1488, 1681, 2179
Van91I CCANNNNNTGG 1 cut(s) 578
VpaK11BI GGWCC 3 cut(s) 153, 251, 1081
XagI CCTNNNNNAGG 1 cut(s) 1089
XapI RAATTY 4 cut(s) 171, 1035, 1391, 1543
XceI RCATGY 4 cut(s) 1181, 1243, 1527, 2102
XcmI CCANNNNNNNNNTGG 3 cut(s) 338, 430, 2011
XhoI CTCGAG 1 cut(s) 1649
XmnI GAANNNNTTC 2 cut(s) 1059, 1563
XspI CTAG 4 cut(s) 1458, 1497, 1632, 2144
Zsp2I ATGCAT 1 cut(s) 1245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.