pycom08g01670

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0005427
Physical Location & Seq
Reverse (-)
1242391 .. 1246964
4574 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2505 bp
ATGGGTGCTCTCGCTCCGTTCTCTAAATGGATTCCGGCGGATGATATCCTGCTTAAGAAAGCTGTTGAGGATGGTGCTTCGTTGGAGTCGCTTGCTAAAGGTGCAGTGCATTTTTCTCGAAGATTTACTGTTCGTGAGCTGCAAGATCGGTGGTATTCTCTCCTGTATGATCCAGTAGTTTCTGAAGATGCTTCTGCTCGCATGATTGAGTTTGAGAGTTCCACCACAACTGTTCCATTTGACAGGTCGGGAAATTCAAAAGAAAAAAAATGCGAATCCGGAAAGAGAAAGGCTGAAAGTGTTCGTAGTAGTTACTATGCGATGCGTAAAAGGATTTGCAATGAGCCATTTAATTCCATGGGCCTTAATTTTCTGGTTGCACCCAGTAATAACAACTATCATGGAAATGGAGATGAGCCTCTTTATGGAAATTGCATGAATGGAGATCCGACCTCAGCCCCTTTTGGCCTTGAGACGTCAGATGTGGATGCTATGCAAAATCTGATGAATGTCGGCATTGATGATACCTTTCATACGCTCCAGAACCCAGATGGGAATGATTTTCATATGGAACAAGGTAATATACATGAAGATATTATGACATGGAATGAATCTGAGGTGGATGAATTCAATCATCCAGAGGGATTGCCAGATTGCAGCCTGTTCAATGCTGATGACTTGGGAATGAAATTTACACTTGATCAAATCAATGGCAACGAGGGAAATATGTGCACAGAATTTGAAGGGAATAAGGCCTTTAATTCACCTGTTTCAGACAGTGGTGCATTGTTTAACAACTTGGAGTATTCATGTCCACTTCCAGCTATGCCAGTCGATGTTGATCTTAGAGAAAATAATATGTGCACCAGCGATGCTTTTGAACTTCATGAGAATATCGATGCTGACAGTGCAAGAACATCAGAGACTGAAGTTCATATTAGGATTGAGGTGGACGCTGAAATGCCATGTGGTGATTTCCAGAGGTCTGCAGCTCCTGTTAGTACTGAAGGTTATTTGGCAGAACTGTCCAATTCACTTTTAAACTTTACGAACGAGGAGCTCATGCTTATGACTGCTGATGGAAAAGATGTGATTGATAAGTCTTACTATGATGGCTTGAGCTCCCTTTTGTTGAGGTCACCGAAAGATGGTGTTCATCAAGAACAGACGATCGTCAAATCTGAGCCAGAAACATCTGTAGCTCCAGTTATGGACCCCATGAATCCCCCTAGTACGTATCCTGGAGTGGTAGATGATAATAGAGGATCCCCAAATGCTGATGAAGATATGGTTTGCCATTCAGAGACTCCGATGCTATCATCTTCAACTGCTTCGAACTATCAAAATCCTGAATTGAAAGATGGAGTTATCTGCTGCCCATTAAACACCGAGGACCCAGAAATTCCATGCAACGATGATATTTTTCTACCAAATCGTCCATCAACTTCTGAAGTGAATAAGCCATTATCTTTGTCCACGAGAGGTTTCCCTGTTAATAAGAGAAATAATGACACAGGGTCATGCTTTATGCACAGAGAGCGAAAAAATCTTGGAGAACCACATAGTACCTCTCAGATTAAAGGATCACACTTCTTACAAGAAAAGGGTCTAAAACCTCCACTAGGTAATTTTGGGGTGAAATTTGAGCTGTCTAAGACTGACACCAATGAAGTGGCATCTAAGAATGCAGGTCATGTTAGTGAAGTTCTGGGTCAAATTTATTCAGCCAATCCAAGTACAAGTGTTCAACCTGGAATCCTGAAGAAAGACACTAGAGAAAAAAGTATATCGGCAAAGCTTCTCAATTATAACTCAACTGAGTTTCATGTGGAGAAACCAGATTCCGGTGTCATTAAACAGGAGCTTGATCCATCAGCTACAATTAGAGACAATAAATCATTACATACAGAAGGGATTGCTGAAGCACAATTAAACCCTGAGACATCAGATCAACTGGGGCTTTTTGAGAGCGATGATGATGTACCATGTTATTCCGACATAGAGGCAATGGTACTTGATATGGATTTGGACCCATATGACCAGGAGTTGTACTCTAGCGAGGAAGTCTCAAAATATCAGAATGAGGATGCTAAGAGGAGAATCATAAGGCTGGAGCAGTGTGCTTATTCATATTTGCAAAGAGCCACTGCATCTCACGGAGCATTTGCCATTTTGTATGGACGTAATTCTAAGCATTACATTAAGAAGCCTGAGGTTTTATTGGGTAGAGCAACAGACGACACTGTTGTTGATATTGACTTGGGAAGTGAAGGGCGTGGTAATAAGATATCTCGAAAACAGGCAATGATAAATATGGACAAAGATGGATCCTTCCATCTGAAAAATCTTGGGAAGTGTTCAATCTCGGTAAATAGCAAGGAGGTAGCCCCCGGACAAAGTCTAAGCCTTAGTTCAAGTTGCTTGATCGAGATACGGGGAATGCCATTCATATTCGAGATGAACCAAACACGCGTGAAGCAGTATTTGGATAGCATCCCGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005783 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0012505 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

835

Amino Acids

92.4

Weight (kDa)

4.69

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCRS_N PF13325 9 - 74 5.5e-15 N-terminal region of micro-spherule protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1809
AatII GACGTC 1 cut(s) 479
Acc36I ACCTGC 1 cut(s) 1679
AccII CGCG 1 cut(s) 2472
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 2 cut(s) 38, 2498
AclWI GGATC 8 cut(s) 164, 440, 1260, 1273, 1591, 1862, 2322, 2335
AcsI RAATTY 7 cut(s) 253, 626, 689, 737, 1401, 1640, 1716
AcuI CTGAAG 6 cut(s) 204, 948, 1026, 1470, 1781, 1941
AcyI GRCGYC 1 cut(s) 476
AfaI GTAC 7 cut(s) 1003, 1234, 1567, 1738, 1983, 2013, 2051
AfiI CCNNNNNNNGG 4 cut(s) 425, 1148, 1622, 1844
AflII CTTAAG 1 cut(s) 53
AflIII ACRYGT 1 cut(s) 2470
AhdI GACNNNNNGTC 1 cut(s) 1516
AjnI CCWGG 3 cut(s) 1240, 1750, 2040
AjuI GAANNNNNNNTTGG 2 cut(s) 2468, 2500
Alw21I GWGCWC 5 cut(s) 10, 734, 866, 1062, 1124
Alw26I GTCTC 6 cut(s) 467, 917, 1298, 1881, 1934, 2071
Alw44I GTGCAC 2 cut(s) 730, 862
AlwI GGATC 8 cut(s) 164, 440, 1260, 1273, 1591, 1862, 2322, 2335
AlwNI CAGNNNCTG 2 cut(s) 926, 995
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 3 cut(s) 361, 466, 753
ApaLI GTGCAC 2 cut(s) 730, 862
ApeKI GCWGC 4 cut(s) 139, 657, 989, 1374
ApoI RAATTY 7 cut(s) 253, 626, 689, 737, 1401, 1640, 1716
Asp700I GAANNNNTTC 1 cut(s) 300
AspS9I GGNCC 4 cut(s) 361, 1213, 1393, 2029
AsuC2I CCSGG 1 cut(s) 2391
AsuHPI GGTGA 4 cut(s) 756, 983, 1131, 1648
AsuII TTCGAA 1 cut(s) 1334
AvaII GGWCC 3 cut(s) 1213, 1393, 2029
AxyI CCTNAGG 1 cut(s) 2211
BaeGI GKGCMC 2 cut(s) 734, 866
BamHI GGATCC 2 cut(s) 1265, 2327
BanII GRGCYC 2 cut(s) 1062, 1124
BauI CACGAG 1 cut(s) 1477
Bbv12I GWGCWC 5 cut(s) 10, 734, 866, 1062, 1124
BbvCI CCTCAGC 1 cut(s) 454
BbvI GCAGC 4 cut(s) 126, 669, 1001, 1361
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 3 cut(s) 1242, 1752, 2042
BciVI GTATCC 1 cut(s) 1248
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 1 cut(s) 2391
BcoDI GTCTC 6 cut(s) 467, 917, 1298, 1881, 1934, 2071
BfaI CTAG 4 cut(s) 1230, 1622, 1773, 2055
BfmI CTRYAG 2 cut(s) 987, 1197
BfrI CTTAAG 1 cut(s) 53
BfuAI ACCTGC 1 cut(s) 1679
BfuI GTATCC 1 cut(s) 1248
BisI GCNGC 4 cut(s) 140, 658, 990, 1375
BlsI GCNGC 4 cut(s) 141, 659, 991, 1376
BmcAI AGTACT 1 cut(s) 1003
Bme1390I CCNGG 4 cut(s) 1242, 1752, 2042, 2391
Bme18I GGWCC 3 cut(s) 1213, 1393, 2029
BmeRI GACNNNNNGTC 1 cut(s) 1516
BmgT120I GGNCC 4 cut(s) 361, 1213, 1393, 2029
BmiI GGNNCC 5 cut(s) 1215, 1267, 1395, 2031, 2329
BmrFI CCNGG 4 cut(s) 1242, 1752, 2042, 2391
BmrI ACTGGG 2 cut(s) 378, 1964
BmsI GCATC 9 cut(s) 178, 312, 478, 862, 889, 1302, 1685, 2077, 2159
BmuI ACTGGG 2 cut(s) 378, 1964
BoxI GACNNNNGTC 1 cut(s) 1172
BplI GAGNNNNNCTC 4 cut(s) 2036, 2068, 2051, 2083
BpmI CTGGAG 4 cut(s) 524, 1188, 1263, 2132
Bpu10I CCTNAGC 1 cut(s) 454
Bpu14I TTCGAA 1 cut(s) 1334
BpuEI CTTGAG 2 cut(s) 491, 1138
BpuMI CCSGG 1 cut(s) 2391
Bsa29I ATCGAT 1 cut(s) 897
BsaAI YACGTR 1 cut(s) 1236
BsaBI GATNNNNATC 1 cut(s) 840
BsaHI GRCGYC 1 cut(s) 476
BsaJI CCNNGG 3 cut(s) 357, 1389, 2389
BsaWI WCCGGW 2 cut(s) 278, 1844
Bsc4I CCNNNNNNNGG 4 cut(s) 425, 1148, 1622, 1844
Bse1I ACTGG 5 cut(s) 173, 384, 830, 1205, 1959
Bse21I CCTNAGG 1 cut(s) 2211
Bse3DI GCAATG 3 cut(s) 346, 2013, 2310
Bse8I GATNNNNATC 1 cut(s) 840
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 3 cut(s) 1242, 1752, 2042
BseCI ATCGAT 1 cut(s) 897
BseDI CCNNGG 3 cut(s) 357, 1389, 2389
BseGI GGATG 7 cut(s) 46, 76, 493, 628, 634, 2092, 2493
BseJI GATNNNNATC 1 cut(s) 840
BseLI CCNNNNNNNGG 4 cut(s) 425, 1148, 1622, 1844
BseMI GCAATG 3 cut(s) 346, 2013, 2310
BseMII CTCAG 7 cut(s) 468, 606, 1173, 1586, 1809, 1929, 2202
BseNI ACTGG 5 cut(s) 173, 384, 830, 1205, 1959
BseRI GAGGAG 2 cut(s) 1070, 2110
BseSI GKGCMC 2 cut(s) 734, 866
BseXI GCAGC 4 cut(s) 126, 669, 1001, 1361
BsgI GTGCAG 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 2472
Bsh1285I CGRYCG 1 cut(s) 1173
BshFI GGCC 3 cut(s) 363, 468, 755
BshVI ATCGAT 1 cut(s) 897
BsiEI CGRYCG 1 cut(s) 1173
BsiHKAI GWGCWC 5 cut(s) 10, 734, 866, 1062, 1124
BsiSI CCGG 4 cut(s) 35, 279, 1845, 2391
BslI CCNNNNNNNGG 4 cut(s) 425, 1148, 1622, 1844
BsmAI GTCTC 6 cut(s) 467, 917, 1298, 1881, 1934, 2071
BsmBI CGTCTC 1 cut(s) 467
BsmI GAATGC 2 cut(s) 1690, 2445
BsnI GGCC 3 cut(s) 363, 468, 755
Bsp119I TTCGAA 1 cut(s) 1334
Bsp1286I GDGCHC 5 cut(s) 10, 734, 866, 1062, 1124
Bsp13I TCCGGA 1 cut(s) 278
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 2 cut(s) 38, 2498
BspANI GGCC 3 cut(s) 363, 468, 755
BspCNI CTCAG 7 cut(s) 467, 607, 1174, 1585, 1810, 1930, 2203
BspDI ATCGAT 1 cut(s) 897
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 1 cut(s) 2472
BspHI TCATGA 1 cut(s) 886
BspLI GGNNCC 5 cut(s) 1215, 1267, 1395, 2031, 2329
BspMAI CTGCAG 1 cut(s) 991
BspMI ACCTGC 1 cut(s) 1679
BspPI GGATC 8 cut(s) 164, 440, 1260, 1273, 1591, 1862, 2322, 2335
BspT104I TTCGAA 1 cut(s) 1334
BspTI CTTAAG 1 cut(s) 53
BsrDI GCAATG 3 cut(s) 346, 2013, 2310
BsrI ACTGG 5 cut(s) 173, 384, 830, 1205, 1959
BssECI CCNNGG 3 cut(s) 357, 1389, 2389
BssNI GRCGYC 1 cut(s) 476
BssSI CACGAG 1 cut(s) 1477
BssT1I CCWWGG 1 cut(s) 357
Bst2BI CACGAG 1 cut(s) 1477
Bst2UI CCWGG 3 cut(s) 1242, 1752, 2042
Bst4CI ACNGT 6 cut(s) 130, 232, 779, 908, 1026, 2245
BstACI GRCGYC 1 cut(s) 476
BstAFI CTTAAG 1 cut(s) 53
BstBAI YACGTR 1 cut(s) 1236
BstBI TTCGAA 1 cut(s) 1334
BstC8I GCNNGC 2 cut(s) 93, 199
BstDSI CCRYGG 1 cut(s) 357
BstEII GGTNACC 1 cut(s) 1137
BstENI CCTNNNNNAGG 1 cut(s) 1620
BstF5I GGATG 7 cut(s) 46, 76, 493, 628, 634, 2092, 2493
BstFNI CGCG 1 cut(s) 2472
BstMAI GTCTC 6 cut(s) 467, 917, 1298, 1881, 1934, 2071
BstMCI CGRYCG 1 cut(s) 1173
BstMWI GCNNNNNNNGC 3 cut(s) 101, 908, 1537
BstNI CCWGG 3 cut(s) 1242, 1752, 2042
BstPAI GACNNNNGTC 1 cut(s) 1172
BstPI GGTNACC 1 cut(s) 1137
BstSCI CCNGG 4 cut(s) 1240, 1750, 2040, 2389
BstSFI CTRYAG 2 cut(s) 987, 1197
BstSLI GKGCMC 2 cut(s) 734, 866
BstSNI TACGTA 1 cut(s) 1236
BstUI CGCG 1 cut(s) 2472
BstV1I GCAGC 4 cut(s) 126, 669, 1001, 1361
BstX2I RGATCY 3 cut(s) 445, 1265, 2327
BstXI CCANNNNNNTGG 1 cut(s) 1672
BstYI RGATCY 3 cut(s) 445, 1265, 2327
Bsu15I ATCGAT 1 cut(s) 897
Bsu36I CCTNAGG 1 cut(s) 2211
BsuI GTATCC 1 cut(s) 1248
BsuRI GGCC 3 cut(s) 363, 468, 755
BsuTUI ATCGAT 1 cut(s) 897
BtgI CCRYGG 1 cut(s) 357
BtgZI GCGATG 3 cut(s) 335, 885, 1986
BtsCI GGATG 7 cut(s) 46, 76, 493, 628, 634, 2092, 2493
BtsI GCAGTG 3 cut(s) 111, 2123, 2145
BtsIMutI CAGTG 6 cut(s) 111, 784, 913, 2123, 2145, 2241
BveI ACCTGC 1 cut(s) 1679
Cac8I GCNNGC 2 cut(s) 93, 199
CaiI CAGNNNCTG 2 cut(s) 926, 995
CciI TCATGA 1 cut(s) 886
Cfr13I GGNCC 4 cut(s) 361, 1213, 1393, 2029
ClaI ATCGAT 1 cut(s) 897
CseI GACGC 1 cut(s) 962
Csp6I GTAC 7 cut(s) 1002, 1233, 1566, 1737, 1982, 2012, 2050
CspCI CAANNNNNGTGG 2 cut(s) 131, 166
CviQI GTAC 7 cut(s) 1002, 1233, 1566, 1737, 1982, 2012, 2050
DraI TTTAAA 1 cut(s) 1041
DriI GACNNNNNGTC 1 cut(s) 1516
Eam1105I GACNNNNNGTC 1 cut(s) 1516
EciI GGCGGA 1 cut(s) 53
Ecl136II GAGCTC 2 cut(s) 1060, 1122
Eco105I TACGTA 1 cut(s) 1236
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 1 cut(s) 755
Eco24I GRGCYC 2 cut(s) 1062, 1124
Eco32I GATATC 2 cut(s) 46, 2289
Eco47I GGWCC 3 cut(s) 1213, 1393, 2029
Eco53kI GAGCTC 2 cut(s) 1060, 1122
Eco57I CTGAAG 6 cut(s) 204, 948, 1026, 1470, 1781, 1941
Eco81I CCTNAGG 1 cut(s) 2211
Eco91I GGTNACC 1 cut(s) 1137
EcoICRI GAGCTC 2 cut(s) 1060, 1122
EcoNI CCTNNNNNAGG 1 cut(s) 1620
EcoO109I RGGNCCY 1 cut(s) 1393
EcoO65I GGTNACC 1 cut(s) 1137
EcoRI GAATTC 1 cut(s) 626
EcoRII CCWGG 3 cut(s) 1240, 1750, 2040
EcoRV GATATC 2 cut(s) 46, 2289
EcoT14I CCWWGG 1 cut(s) 357
EcoT38I GRGCYC 2 cut(s) 1062, 1124
ErhI CCWWGG 1 cut(s) 357
Esp3I CGTCTC 1 cut(s) 467
FauNDI CATATG 2 cut(s) 567, 2035
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 4 cut(s) 140, 658, 990, 1375
FokI GGATG 7 cut(s) 53, 83, 500, 621, 635, 2099, 2480
FriOI GRGCYC 2 cut(s) 1062, 1124
Fsp4HI GCNGC 4 cut(s) 140, 658, 990, 1375
FspBI CTAG 4 cut(s) 1230, 1622, 1773, 2055
GluI GCNGC 4 cut(s) 140, 658, 990, 1375
GsuI CTGGAG 4 cut(s) 524, 1188, 1263, 2132
HaeIII GGCC 3 cut(s) 363, 468, 755
HapII CCGG 4 cut(s) 35, 279, 1845, 2391
HgaI GACGC 1 cut(s) 962
Hin1I GRCGYC 1 cut(s) 476
HindIII AAGCTT 1 cut(s) 1796
HinfI GANTC 9 cut(s) 31, 86, 275, 611, 1222, 1306, 1755, 1841, 2100
HpaII CCGG 4 cut(s) 35, 279, 1845, 2391
HphI GGTGA 4 cut(s) 756, 983, 1131, 1648
Hpy166II GTNNAC 5 cut(s) 732, 815, 864, 952, 1476
Hpy8I GTNNAC 5 cut(s) 732, 815, 864, 952, 1476
HpyAV CCTTC 5 cut(s) 737, 1001, 1904, 2264, 2341
HpyCH4III ACNGT 6 cut(s) 130, 232, 779, 908, 1026, 2245
HpyCH4IV ACGT 3 cut(s) 476, 1235, 2182
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 908, 1537
HpySE526I ACGT 3 cut(s) 476, 1235, 2182
Hsp92I GRCGYC 1 cut(s) 476
Kpn2I TCCGGA 1 cut(s) 278
Ksp22I TGATCA 1 cut(s) 700
LmnI GCTCC 9 cut(s) 19, 543, 997, 1057, 1127, 1207, 1861, 2113, 2159
Lsp1109I GCAGC 4 cut(s) 126, 669, 1001, 1361
LweI GCATC 9 cut(s) 178, 312, 478, 862, 889, 1302, 1685, 2077, 2159
MaeI CTAG 4 cut(s) 1230, 1622, 1773, 2055
MaeII ACGT 3 cut(s) 476, 1235, 2182
MaeIII GTNAC 2 cut(s) 311, 1137
MboII GAAGA 6 cut(s) 132, 197, 602, 1295, 1314, 1774
MflI RGATCY 3 cut(s) 445, 1265, 2327
MhlI GDGCHC 5 cut(s) 10, 734, 866, 1062, 1124
MluI ACGCGT 1 cut(s) 2470
MlyI GAGTC 2 cut(s) 95, 1300
MmeI TCCRAC 3 cut(s) 63, 473, 2019
MroI TCCGGA 1 cut(s) 278
MroXI GAANNNNTTC 1 cut(s) 300
MslI CAYNNNNRTG 3 cut(s) 405, 1067, 1698
MspCI CTTAAG 1 cut(s) 53
MspI CCGG 4 cut(s) 35, 279, 1845, 2391
MspR9I CCNGG 4 cut(s) 1242, 1752, 2042, 2391
Mva1269I GAATGC 2 cut(s) 1690, 2445
MvaI CCWGG 3 cut(s) 1242, 1752, 2042
MvnI CGCG 1 cut(s) 2472
MwoI GCNNNNNNNGC 3 cut(s) 101, 908, 1537
NciI CCSGG 1 cut(s) 2391
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 2 cut(s) 567, 2035
NlaIV GGNNCC 5 cut(s) 1215, 1267, 1395, 2031, 2329
NmuCI GTSAC 1 cut(s) 1137
NspV TTCGAA 1 cut(s) 1334
PagI TCATGA 1 cut(s) 886
PceI AGGCCT 1 cut(s) 755
PctI GAATGC 2 cut(s) 1690, 2445
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 7 cut(s) 31, 275, 611, 1222, 1755, 1841, 2100
PflFI GACNNNGTC 1 cut(s) 2397
PfoI TCCNGGA 1 cut(s) 1240
PkrI GCNGC 4 cut(s) 141, 659, 991, 1376
Ple19I CGATCG 1 cut(s) 1173
PleI GAGTC 2 cut(s) 94, 1300
PpsI GAGTC 2 cut(s) 94, 1300
Ppu21I YACGTR 1 cut(s) 1236
PpuMI RGGWCCY 1 cut(s) 1393
PshAI GACNNNNGTC 1 cut(s) 1172
PsiI TTATAA 1 cut(s) 1809
Psp124BI GAGCTC 2 cut(s) 1062, 1124
Psp5II RGGWCCY 1 cut(s) 1393
Psp6I CCWGG 3 cut(s) 1240, 1750, 2040
PspEI GGTNACC 1 cut(s) 1137
PspGI CCWGG 3 cut(s) 1240, 1750, 2040
PspN4I GGNNCC 5 cut(s) 1215, 1267, 1395, 2031, 2329
PspPI GGNCC 4 cut(s) 361, 1213, 1393, 2029
PspPPI RGGWCCY 1 cut(s) 1393
PstI CTGCAG 1 cut(s) 991
PstNI CAGNNNCTG 2 cut(s) 926, 995
PsuI RGATCY 3 cut(s) 445, 1265, 2327
PsyI GACNNNGTC 1 cut(s) 2397
PvuI CGATCG 1 cut(s) 1173
RsaI GTAC 7 cut(s) 1003, 1234, 1567, 1738, 1983, 2013, 2051
RsaNI GTAC 7 cut(s) 1002, 1233, 1566, 1737, 1982, 2012, 2050
RseI CAYNNNNRTG 3 cut(s) 405, 1067, 1698
SacI GAGCTC 2 cut(s) 1062, 1124
SatI GCNGC 4 cut(s) 140, 658, 990, 1375
Sau96I GGNCC 4 cut(s) 361, 1213, 1393, 2029
ScaI AGTACT 1 cut(s) 1003
SchI GAGTC 2 cut(s) 95, 1300
ScrFI CCNGG 4 cut(s) 1242, 1752, 2042, 2391
SduI GDGCHC 5 cut(s) 10, 734, 866, 1062, 1124
SfaNI GCATC 9 cut(s) 178, 312, 478, 862, 889, 1302, 1685, 2077, 2159
SfcI CTRYAG 2 cut(s) 987, 1197
SfuI TTCGAA 1 cut(s) 1334
SinI GGWCC 3 cut(s) 1213, 1393, 2029
SmiMI CAYNNNNRTG 3 cut(s) 405, 1067, 1698
SmlI CTYRAG 3 cut(s) 53, 470, 1117
SmoI CTYRAG 3 cut(s) 53, 470, 1117
SnaBI TACGTA 1 cut(s) 1236
SseBI AGGCCT 1 cut(s) 755
SsiI CCGC 2 cut(s) 38, 2498
SspMI CTAG 4 cut(s) 1230, 1622, 1773, 2055
SstI GAGCTC 2 cut(s) 1062, 1124
StuI AGGCCT 1 cut(s) 755
StyD4I CCNGG 4 cut(s) 1240, 1750, 2040, 2389
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 6 cut(s) 130, 232, 779, 908, 1026, 2245
TaiI ACGT 3 cut(s) 479, 1238, 2185
TaqI TCGA 7 cut(s) 118, 834, 897, 1334, 2293, 2427, 2454
TatI WGTACW 3 cut(s) 1001, 1736, 2049
TfiI GAWTC 7 cut(s) 31, 275, 611, 1222, 1755, 1841, 2100
TscAI CASTG 6 cut(s) 111, 784, 913, 2123, 2152, 2248
TseFI GTSAC 1 cut(s) 1137
TseI GCWGC 4 cut(s) 139, 657, 989, 1374
Tsp45I GTSAC 1 cut(s) 1137
TspGWI ACGGA 2 cut(s) 6, 2172
TspRI CASTG 6 cut(s) 111, 784, 913, 2123, 2152, 2248
Tth111I GACNNNGTC 1 cut(s) 2397
Vha464I CTTAAG 1 cut(s) 53
VneI GTGCAC 2 cut(s) 730, 862
VpaK11BI GGWCC 3 cut(s) 1213, 1393, 2029
XagI CCTNNNNNAGG 1 cut(s) 1620
XapI RAATTY 7 cut(s) 253, 626, 689, 737, 1401, 1640, 1716
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 4 cut(s) 1230, 1622, 1773, 2055
ZraI GACGTC 1 cut(s) 477
ZrmI AGTACT 1 cut(s) 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.